KR880002453B1 - Improved process for a ball of beam paste of ordinary soy - Google Patents

Improved process for a ball of beam paste of ordinary soy Download PDF

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KR880002453B1
KR880002453B1 KR1019860000874A KR860000874A KR880002453B1 KR 880002453 B1 KR880002453 B1 KR 880002453B1 KR 1019860000874 A KR1019860000874 A KR 1019860000874A KR 860000874 A KR860000874 A KR 860000874A KR 880002453 B1 KR880002453 B1 KR 880002453B1
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meju
bacillus subtilis
soy sauce
improved
fermented
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KR880000022A (en
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이한창
엄기승
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이한창
엄기승
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof

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  • Agronomy & Crop Science (AREA)
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  • Food Science & Technology (AREA)
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  • Soy Sauces And Products Related Thereto (AREA)

Abstract

A method for preparing improved soybean cake for traditional soybean sauce is described. Thus, 10 kg soybean was immersed into H2O at room temp. for 10 hr and steamed at 0.5 kg/cm2 for 30 min. The material was cooled to 30↿C, mixed with 2 g Aspergillus oryzae, fermented at 30-35↿C for 20 hr, and sprayed with 1 g Bacillus subtilis in 200 ml heated H2O to give 9.3 kg improved soybean cake after 30 hr.

Description

재래식 간장 제조용 개량메주의 제조방법Manufacturing Method of Improved Meju for Traditional Soy Sauce Production

본 발명은 한국적인 고유의 풍미를 맛볼 수 있는 재래식 간장 제조용 개량메주의 제조방법에 관한 것이다. 본 발명은 특히 황국균(Aspergillus oryzal)과 고초균(Bacillus subtilis) 또는 납두균(Bacillus natto)으로 시차발효(時差醱酵)시킴으로서 단백질 분해력이 우수하고 악취가 없으며, 또 한국적인 고유의 풍미를 맛볼 수 있는 재래식 간장 제조용 개량메주의 제조방법에 관한 것이다.The present invention relates to a method for producing improved meju for producing conventional soy sauce, which can taste Korean unique flavor. In particular, the present invention is excellent in protein degrading power, no odor, and tastes traditional Korean flavor by staggering fermentation with Aspergillus oryzal and Bacillus subtilis or Bacillus natto. The present invention relates to a method for producing soybean meju.

종래의 재랙식 메주의 제조방법에서 야생균을 이용한 관계로 제품메주에서 때로는 마이코톡신(Mycotoxin)등의 유독성 물질이 검출되거나, 증식 미생물상(相)에도 재현성이 없는등 극히 비위생적 또는 비과학적이며, 또 이에 따른 풍미저하를 감수해야만 하였다.Due to the use of wild bacteria in the conventional method of producing reclaimed meju, toxic substances such as mycotoxin are sometimes detected in the product meju, or they are extremely unhygienic or unscientific. As a result, the flavor had to be reduced.

따라서, 이와 같은 재래식 메주의 단점을 개량하는 한 방편으로 황국균을 이용해서 제조한 소위 "개량메주"가 시판되기에 이르렀다. 이같은 개량메주는 전술한 재래식 메주의 단점들을 개선하는 이외에도 재래식 메주에서 항상 느낄 수 있었던 악취를 제거할 수는 있었으나, 황국균은 일본식 간장 제조시에 사용되는 곰팡이의 일종으로서, 이 개량메주로 제조한 간장을 일본식 간장의 풍미를 갖게 되어 우리국민이 전통적으로 식용해 오고 있는 재래식 간장의 풍미를 얻을 수 없다는 단점이 있었다. 따라서 한국인의 식생활에 있어 필수적인 국을 끓이는데는 일본식 간장은 적합치 못하며, 재래식 간장만이 끓일수록 국의 맛을 돋궈주게 된다는 사실을 감안할 때 이같은 단점은 필히 해결해야만 할 과제이었다.Therefore, as a way of improving the disadvantages of such conventional meju, so-called "improved meju" manufactured using Hwang Kuk bacteria has come to market. In addition to improving the above-mentioned disadvantages of conventional meju, the modified meju was able to remove the odor that was always felt in conventional meju. However, Hwang Kuk-kyun is a type of mold used in making Japanese soy sauce. There was a drawback that it was not possible to get the flavor of the traditional soy sauce that Koreans have traditionally eaten since it has the flavor of Japanese soy sauce. Therefore, Japanese soy sauce is not suitable for cooking soup, which is essential for Koreans' diet, and this problem must be solved, considering that only traditional soy sauce boils up the taste of soup.

본 발명자 등은 전술한 재래식 메주와 개량메주의 장점만을 취해서, 그 제조기일 짧고, 단백질 분해력이 우수하며, 또 악취가 없는 이외에 재래식 메주의 장점을 살릴 수 있는 메주의 제조방법에 대해 연구를 한 결과 본 발명을 완성할 수 있었다.The present inventors took only the advantages of the conventional meju and improved meju as described above, and the result of the study on the method of producing meju, which is short in the production date, excellent in protein decomposition ability, and has no odor, and can utilize the advantages of the conventional meju. The present invention could be completed.

본 발명에 따른 재래식 간장 제조용 개량메주의 제조방법은 원료 대두를 공지의 방법으로 수세, 침지 및 증자를 행하고 약 30℃로 냉각해서 황국균을 첨가 배합한 후에 약 30 내지 35℃ 범위의 국실에서 1차로 황색포자 착생직전까지 숙성 발효시키고 ; 여기에 고초균 또는 납두균을 첨가 배합하고 포화 습도하의 약 40 내지 45℃ 범위의 국실에서 약 20 내지 30시간 동안 2차 숙성 발효시킨후 ; 수분함량 15% 내외로 건조시키는 단계로 구성된다.According to the present invention, a method of preparing improved meju for preparing conventional soy sauce is primarily washed in raw materials in a range of about 30 to 35 ° C. after the raw soybeans are washed, immersed, and cooked in a known manner, and cooled to about 30 ° C. to further add sulfuric acid bacteria. Fermentation is carried out until just before yellow spore engraftment; After adding and adding Bacillus subtilis or Bacillus subtilis to the second aged fermentation for about 20 to 30 hours in the domestic room in the range of about 40 to 45 ℃ under saturated humidity; It is composed of a step of drying to around 15% moisture content.

증자대두에 황국균을 첨가 혼합한 배합물을 국실에 장입한 후 10 내지 12시간이 경과하면 황국균포저가 발아함에 따라 발열이 시작된다. 이때 황국균증식에 가장 바람직한 온도인 30 내지 35℃로 조정해 주어야만 한다. 그러면 시간이 경과함에 따라 육안으로 식별 가능한 백색의 균사가 생성되며, 황국균 특유의 향기가 발생된다. 시간이 더 경과하면 황색포자가 착생하게 되는데 본 발명에서는 이 황색포자 착생직전에 고초균을 첨가 발효시킴을 그 요지로 하고 있다. 황색포자의 착생시점은 국실의 보온성과 습도 및 온도조건에 따라 다소차이는 있으나, 국실 장입후 15 내지 20시간이 되도록 제반조건을 조절하는 것이 바람직하다.10 to 12 hours after charging the mixture with the addition of Rheum bacteria in cooked soybeans to the soup room, fever starts as the Rheum oyster germination germinates. At this time, it should be adjusted to 30 to 35 ℃ which is the most preferable temperature for the growth of the bacterium. Then, over time, white mycelium that can be identified with the naked eye is generated, and a unique scent of yellow bacteria is generated. As time passes, yellow spores grow, but the present invention is intended to add fermentation of Bacillus subtilis immediately before the growth of yellow spores. The time of engraftment of the yellow spores is somewhat different depending on the warmth and humidity and temperature conditions of the soup room, but it is preferable to adjust the conditions so that it is 15 to 20 hours after loading the room.

본 발명에 따라 제조한 메주는 황국균 및 고초균을 각각 사용해서 제조한 메주에 비해서 프로테아제 역가가 월등히 증대하였다. 즉, 가께야마씨법(陰山氏法)으로 측정한 고초균메주와 황국균메주의 프로테아제 역가는 각각 2.83 및 3.01로서, 이들 혼합 메주의 프로테아제 역가가 5.84였음에 반해서, 본 발명 메주의 프로테아제 역가는 8.02(황국균 배양과정 2.75 ; 고초균 배양과정 4.27)이었다.The meju prepared according to the present invention significantly increased the protease titer compared to the meju prepared using the H. pylori and Bacillus subtilis, respectively. In other words, the protease titers of Bacillus subtilis and Hwang Kyun meju were measured by the Kekayama method and were 2.83 and 3.01, respectively, while the protease titers of the mixed meju were 5.84. (Hookuk bacteria culture process 2.75; Bacillus subtilis culture process 4.27).

이같이 본 발명의 고초균 배양과정에서 프로테아제 역가가 급격히 증대하게 되는 것은 전단계인 황국균 배양과정에서 생성된 비타민류 및 유리 아미노산류 등이 고초균의 영양소로 작용하여 고초균의 증식에 기여하였기 때문인 것으로 사료된다. 황국균은 증량의 목적 또는 메주제품의 특성 부여를 목적으로 볶아서 타겐밀 또는 보리가루의 적당량에 혼합한 후 사용할 수 있다.As such, the protease titer rapidly increases in the Bacillus cultivation process of the present invention because vitamins and free amino acids generated during the cultivation of the Bacillus bacterium act as nutrients of Bacillus subtilis and contribute to the growth of Bacillus subtilis. It can be used after roasting for the purpose of increasing the weight or for characterizing meju's products and mixing it with an appropriate amount of targent wheat or barley flour.

고초균을 첨가한 후 2차 발효를 약 20시간 내지 30시간 행하면, 무틴질이 생성되어 혼합물의 끈기가 발생하게 된다.When the secondary fermentation is performed for about 20 to 30 hours after the addition of Bacillus subtilis, mutin is produced and the persistence of the mixture occurs.

본 발명 메주제품은 수분조건에 앞서 혼합물을 마쇄기로 마쇄하고 메주모양으로 성형한 후 건조할 수도 있으나, 통상은 그대로 건조시키서 대두형상을 유지해서 제조한다.The meju product of the present invention may be dried after grinding the mixture with a mill and shaping the meju prior to the moisture condition, but is usually dried as it is to maintain the soybean shape.

본 발명에서 사용되는 균주는 배양균(Type Culture) 또는 야생균(Wild culture)의 구별없이 사용될 수 있다.Strains used in the present invention can be used without distinction between type culture or wild culture.

본 발명 방법에 따라 제조한 재래식 간장 제조용 개량메주는 그 제조기일이 불과 2 내지 3일 정도로 종래의 재래식 메주의 제조기일인 1 내지 3개월에 비해서 그 기간을 현저하게 단축시킬 수 있다. 이밖에 증식미생물상의 재현성이 양호하고, 유독성 물질이 검출되지 않으며, 재래식 메주에서와 같은 악취 또한 제거할 수 있다. 이밖에도 본 발명 개량메주를 사용해서 간장을 제조하는 경우에는 재래식 간장 고유의 풍미를 수득할 수 있다.The improved meju for producing soy sauce prepared according to the method of the present invention can be shortened significantly compared to 1 to 3 months, which is the manufacturing date of the conventional conventional meju, to about 2 to 3 days. In addition, the reproducibility of proliferating microorganisms is good, no toxic substances are detected, and odors as in conventional meju can be removed. In addition, when manufacturing soy sauce using the improved meju of the present invention, flavors inherent in conventional soy sauce can be obtained.

본 발명은 다음 실시예로서 보다 상세히 설명된다.The invention is explained in more detail by the following examples.

[실시예 1]Example 1

석발제거, 수세를 행한 원료대두 10㎏을 상온의 물에 10시간 침지한 후 0.5㎏/㎠의 수증기로 30분간 증자하고 약 30℃로 냉각하였다. 여기에 황국균 2g을 첨가 배합한 후에 약 30 내지 35℃에서 20시간 배양해서 백색의 균사가 증식되었을 때 고초균 1g을 열수 200㎖에 현탁시킨 현탁액을 균일하게 분무 혼합해 주고 포화습도하에서 온도를 40 내지 45℃로 승온시키고 이 온도를 30시간 유지하였다. 이어 배합물을 수분함량 15%내외로 건조시켜서 대두 형상의 본 발명 개량메주 9.3㎏(A제품)을 수득하였다.10 kg of the raw soybeans that had been removed and washed with water were immersed in water at room temperature for 10 hours, and then steamed for 30 minutes with 0.5 kg / cm 2 of steam and cooled to about 30 ° C. After adding and mixing 2 g of Rheum bacteria, the mixture was incubated at about 30 to 35 ° C. for 20 hours, and when white mycelium was grown, uniformly spray-mix the suspension of 1 g of Bacillus subtilis suspended in 200 ml of hot water, and then heated the temperature to 40 to 40 ° C under saturated humidity. The temperature was raised to 45 ° C. and maintained at this temperature for 30 hours. The blend was then dried to around 15% moisture content to obtain 9.3 kg (product A) of the inventive improved meju in soybean shape.

[실시예 2]Example 2

실시예 1에서 수분을 건조하기 직전에 배합물을 마쇄기로 약 800메쉬로 마쇄하고 성혀 통풍 건조시켜서 재래식 메주모양의 본 발명 개량메주 9.6㎏(B제품)을 수득하였다.In Example 1, the blend was ground to about 800 mesh with a milling machine immediately before drying the moisture, and then dried by air drying to obtain 9.6 kg (B product) of the present invention improved meju in the shape of a conventional meju.

[실시예 3]Example 3

고초균 발효를 생략하고 또 황국균 발효를 황색포자가 증식될 때까지 계속한 것 이외에는 실시예 1에서와 동일하게 개량메주 9.3㎏(C제품)을 수득하였다.9.3 kg (C product) of improved meju was obtained in the same manner as in Example 1, except that the superbacterial fermentation was omitted and the yellow bacterium fermentation was continued until yellow spores were grown.

[실시예 4]Example 4

황국균 발효를 생략하고 고초균 발효만을 실시한 이외에는 실시예 1에서와 동일하게 개량메주 9.3㎏(D제품)을 수득하였다.An improved meju 9.3 kg (D product) was obtained in the same manner as in Example 1 except that the bacterium fermentation was omitted and only Bacillus subtilis was fermented.

[실시예 5]Example 5

실시예 1,2 및 3에서 제조한 메주제품(A,B,C)과 별도 제조한 재래식 메주 각각 9㎏씩을 사용 공지의 방법에 따라 재래식 간장(I)(Ⅱ)(Ⅲ) 및 (Ⅳ)을 제조하고 관능시험을 실시하였다. 관능시험의 대상인원은 12명으로 하고 간장의 맛에 따라* * * *,* * *,* *,*의 등급을 부여하였다. 시험결과는 다음 표 1에 수록하였다.9 kg each of the meju products (A, B, C) prepared in Examples 1, 2, and 3 and the conventional meju prepared separately were used. It was prepared and subjected to the sensory test. The subjects of sensory evaluation were 12 people. * * * * , * * * , * * , * Test results are listed in Table 1 below.

[표 1]TABLE 1

Figure kpo00001
Figure kpo00001

상기 시험결과로부터 본 발명 개량메주는 황국균만을 사용해서 만든 개량메주는 물론 재래식 메주보다도 간장제조시에 탁월한 효과가 있음을 알 수 있다.From the above test results, it can be seen that the improved meju of the present invention has an excellent effect in making soy sauce than the conventional meju as well as the improved meju made using only the yellow germ bacteria.

[실시예 6]Example 6

실시예 3에서 제조한 황국균 발효 메주 4.5㎏ 및 실시예 4에서 제조한 고초균 발효 메주 4.5㎏을 혼합한 혼합메주와 본 발명 메주 9㎏을 각각 사용 공지의 방법에 따라 재래식 간장(Ⅴ) 및 (I)을 제조하고 제조 30일, 60일, 90일 후의 총질소량을 켈타르씨법에 따라 측정하였다. 측정결과를 다음 표 2에 수록하였다.Mixing meju mixed with 4.5 kg of H. pylori fermented meju prepared in Example 3 and 4.5 kg of Bacillus subtilis fermented meju prepared in Example 4, and 9 kg of the meju of the present invention, respectively, using conventional soy sauce (V) and (I) ), And the total nitrogen after 30 days, 60 days, and 90 days of manufacture was measured according to the Keltar Seed Method. The measurement results are listed in Table 2 below.

[표 2 : 간장의 시차별 총질소량 비교]Table 2: Comparison of Total Nitrogen Amounts by Soy Sauce]

Figure kpo00002
Figure kpo00002

Claims (1)

원료대두를 공지의 방법으로 수세 침지 및 증자를 행하고 약 30℃로 냉각해서 황국균(Aspergillus oryzal)을 첨가 배합한 후에 약 30 내지 35℃ 범위의 국실에서 1차로 황색포자 착생직전까지 숙성 발효시키고 ; 여기에 추가로 고초균(Bacillus subtilis) 또는 납두균(Bacillus natto)을 첨가 배합하고 포화 습도하 약 20 내지 45℃ 범위의 국실에서 약 20 내지 30시간 동안 2차 숙성 발효시킨후 ; 수분함량 15% 내외로 건조시킴을 특징으로 하는 재래식 간장 제조용 개량메주의 제조방법.The raw soybeans are immersed and washed with water in a known manner, cooled to about 30 ° C., and added with Aspergillus oryzal, and then fermented and fermented to the first in the domestic room in the range of about 30 to 35 ° C. until the yellow spores are grown. Bacillus subtilis or Bacillus natto is further added thereto, followed by secondary aging fermentation for about 20 to 30 hours in a domestic room in the range of about 20 to 45 ° C. under saturated humidity; A method for producing improved meju for producing conventional soy sauce, characterized by drying at about 15% moisture content.
KR1019860000874A 1986-02-07 1986-02-07 Improved process for a ball of beam paste of ordinary soy KR880002453B1 (en)

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Publication number Priority date Publication date Assignee Title
KR101023157B1 (en) * 2008-11-11 2011-03-18 경상대학교산학협력단 Fermented Soybean Product Prepared by Rapid Multi-step Fermentation and Method of Preparation Therefor

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KR100470735B1 (en) * 2001-11-08 2005-02-21 경상대학교산학협력단 A novel fermented soybeans preparated by three steps fermentation using bacteria and method of preparation thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101023157B1 (en) * 2008-11-11 2011-03-18 경상대학교산학협력단 Fermented Soybean Product Prepared by Rapid Multi-step Fermentation and Method of Preparation Therefor

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