KR880000348B1 - Preparation of composition for rice-plant fever - Google Patents

Abstract

An antifungal substance contg. caproic, bytric, n-valeric and iso- valeric acids was extd. from palslane (Portulacae Herba) and used for treating rice blast desease. Thus, palslane was steamboiled for 20- 30min at 100≰C > and pressed with a compressor to give palslance concentrate. The concentrate was extd. with MeOH and vacuum-concd.. The concd. ext. was dissolved in water. Water-sol. fraction was adjusted with HCl to pH 3.0 and extd. with ethylacetate and vacuum- concd.. The concd. ext. was extd. with nhexane, vacuum-concd., applied to Wacogel C-200 column, and eluted with n-hexane/ ethylacetate solvent system. The elute was pooled, concd., extd. with acetone, and concd. to give a white, oily material.

Description

Method for preparing a cure for febrile disease consisting of caproic acid, butyric acid, n-valeric acid and isovaleric acid

The present invention relates to a method for the preparation of a therapeutic agent for rice blasts of rice consisting of caproic acid, butyric acid, n-valeric acid and isovaleric acid.

Various pesticides have been used to prevent or treat rice blasts, but since these pesticides were chemical preparations, they were very toxic or weakened by farmers' mouths, skin or aspirations. As a result, not only did the units of pesticides increase year by year, but also natural disasters caused extinction and caused great changes in the ecosystem.

In addition, there was a drawback of high recurrence rate or low coalescence rate even when spraying drugs to prevent or treat febrile diseases, and pedan, which was further increased by waste or gas from chemical plants for producing pesticides.

The present invention is to correct the conventional pedan at the same time and at the same time as a raw material of wild grasses that are grown a lot in the mountain of our country as raw materials, there is no harm to the killing rate, there is no rate and no method of manufacturing an antibacterial cure of an ideal herbal medicine It is about.

Moreover, the therapeutic agent according to the present invention exhibits an excellent effect, in particular, in the treatment of rice blast, and in addition, it has an excellent effect on sesame plaque, ear bud disease, brown leaf blight, apple blight and pear tree black.

In particular, the therapeutic agent according to the present invention is a therapeutic agent having excellent industrial properties in that it has a high cure rate and no harm to plants such as rice, but also has a weakness in human beings.

Referring to the method for producing a therapeutic agent for febrile disease according to the present invention in detail.

[Step 1]

: Portulacae Herba 일명 쇠비름)을 발육이 제일 양호한 7-8월경에 채취하여 뿌리를 제거하고 잘게 절단, 분쇄하여 저장탱크에 넣어 35℃-55℃의 온도로 24시간 마다 상하좌우로 잘 교반하면서 약 20일간 자연발효시킨 후, 찌꺼기가 나오지 않을 정도의 망을 사용하여 압축기로 압착하여 농축액을 추출한다.Machi prefecture wildly in Korea's mountains and fields

: Portulacae Herba (aka Purslane) is harvested around July-August, which is best for development, and the roots are removed, finely chopped and pulverized and placed in a storage tank at a temperature of 35 ℃ -55 ℃ for 24 hours. After natural fermentation for a day, the extract is compressed by compressing with a compressor using a net that does not come out of the residue.

Instead of such a process, put the gusset into a large cauldron and steam it for 20-30 minutes with steam of 100 ℃ or more, or pour about 1/4 of water and boil it for 20-30 minutes at 100 ℃, then press it with a compressor. The extract can be extracted and fermented for 20 days at 35 ℃ -55 ℃ in a storage tank.

[Step 2]

Methanol was added to the concentrated solution obtained by the above-mentioned process to extract the dissolved component, concentrated under reduced pressure, the water was added to the concentrated solution, the dissolved portion was taken to adjust pH to 3.0 with hydrochloric acid, and extracted with ethyl acetate. Concentration under reduced pressure gives a concentrate.

[Step 3]

N-hexane is added to the concentrate to extract the dissolved components, and the residue is concentrated under reduced pressure, followed by silicate column chromatography.

At this time, the column filler is "Wacogel C-200".

[Step 4]

Combine the extracted portions using n-hexane and n-hexane: ethyl acetate (8: 2) as the extraction solution, concentrate, extract the dissolved portion by adding acetone, and concentrate the extract to obtain gas liquid chromatography. The overall process chart is shown in Table 1 and Table 2 shows the analysis results.

Table 1

Separation Process

The apparatus and conditions used in this separation process are as follows.

Device: Shimadzu model GC-4B

Detector: Hydrogen flame ionization detector

Column: Shimalite (60-80 mesh) coated 15% BEGS film 2m glass helix shaped tube, 4mm diameter)

Column Temperature: 130 ° (150 °) -170 ° (185 °)

Carrier gas: Nitrogen

Flow rate: 40ml / min

Before gas-liquid chromatography, the white oily substance was Rf 0.8 and BPB was positive in thin layer chromatography.

(Fixed phase, silica gel: developer, n-hexane: ethyl acetate (8: 2).

Infrared spectrophotometer showed the largest absorption at 1650cm ^-1 and ultraviolet spectrophotometer showed in-pole absorption at 281nm.

As a result of the above analysis, the antimicrobial substance (active ingredient) obtained by special treatment of Machi (aka purslane) was caproic acid [CH ₃ (CH ₂ ) ₄ COOH], butyric acid [CH ₃ CH ₂ CH ₂ COOH] n- valeric acid Four types of fatty acids were identified: [CH ₃ CH ₂ CH ₂ COOH] and isovaleric acid [(CH ₃ ) ₂ CH ₂ CH ₂ COOH].

The result of treatment of rice blast with rice oil obtained in this way was as follows.

(Paddy rice with blast disease was treated and treated with a microscope after wet treatment in a thermostat at 26 ℃ for 48 hours)

Lesion site spore residual amount by pharmaceutical treatment

Remark: Less than 100 micro-spores

100-500 small spores

Medium to spores 500-1000

More than 1000 multi-spores

Claims (5)

Grind machi finely into a storage tank, fermented and compressed to extract the concentrated solution, methanol is added thereto to extract the dissolved components, concentrated under reduced pressure and water is added thereto to pH 3.0 with hydrochloric acid. The mixture was extracted three times with ethyl acetate, concentrated under reduced pressure, n-hexane was added to the concentrate, the dissolved portion was extracted, concentrated under reduced pressure, silicate column chromatography was carried out, and n-hexane and n-hexane: ethyl were extracted as solvents. Using (8: 2), the extracted portions are combined, concentrated, and extracted by dissolving the added portion by adding acetone. The extract is then subjected to gas liquid chromatography, characterized in that caproic acid, butyric acid, and n-valeric. A method for producing a cure for febrile disease consisting of acid and isovaleric acid. The method of claim 1, wherein the temperature is 35 ℃-55 ℃ in the storage tank, characterized in that the natural fermentation for 20 days with stirring every 24 hours. The method according to claim 1 or 2, characterized in that steaming finely pulverized chopped strings for 20-30 minutes with water vapor of 100 ° C. or higher in a storage tank. The method according to claim 1 or 2, characterized in that, in a storage tank, pour 1/4 of water with respect to the amount of gusset string and boil at 100 ° C for 20-30 minutes. The method of claim 1, wherein the column filler is used "Wacogel C-200".