KR870011248A - Mass production method of human β-interferon - Google Patents

Mass production method of human β-interferon Download PDF

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KR870011248A
KR870011248A KR1019860003834A KR860003834A KR870011248A KR 870011248 A KR870011248 A KR 870011248A KR 1019860003834 A KR1019860003834 A KR 1019860003834A KR 860003834 A KR860003834 A KR 860003834A KR 870011248 A KR870011248 A KR 870011248A
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interferon
human
ifn
sequence
linker
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KR900007868B1 (en
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조중명
이태호
이태규
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주식회사 럭키
허신구
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract

내용 없음No content

Description

인간 β-인터페론의 대량 생산방법Mass production method of human β-interferon

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제 1 도는 β-인터페론 유전자를 대장균 발현 운반체로 제조합시키는 과정을 나타낸 것이고,Figure 1 shows the process of preparing and synthesizing the β-interferon gene E. coli expression carrier,

제 2 도는 시간에 따른 β-인터페론 유전자를 지닌 대장균의 성장곡선 및 생물학적 활성도를 나타낸 것으로,Figure 2 shows the growth curve and biological activity of Escherichia coli carrying β-interferon gene over time,

-0-은 β-인터페론 유전자를 지닌 대장균의 성장곡선을 나타낸 것이고,-0- represents the growth curve of Escherichia coli carrying the β-interferon gene,

-0-은 IPTC로 3시간 유도시킨 후 측정한 β-인터페론의 생물학적 활성도를 나타낸 것이다.-0- represents the biological activity of β-interferon measured after 3 hours of induction by IPTC.

제 3 도는 대장균에서 대량 발현된 β-인터페론을 SDS-폴리아크릴이마이드겔 전기영동 및 웨스턴블라팅(Western Blotting)방법으로 확인한 결과를 각각 나타낸 것이다.3 shows the results of confirming the β-interferon mass expressed in E. coli by SDS-polyacrylamide gel electrophoresis and Western blotting.

Claims (5)

β-인터페론 유전자만을 클로닝시킨 대장균 발현 운반체(ptac5I-100h β-IFN)에 인공합성 링커를 삽입시켜 대장균 발현 운반체(ptac5IS3 IFN)를 제조한 다음 이를 유도 발현시킴을 특징으로 하는 인간 β-인터페론 의 제조방법.Preparation of human β-interferon characterized by preparing an E. coli expression carrier (ptac5IS3 IFN) by inserting an artificial synthetic linker into the E. coli expression carrier (ptac5I-100h β-IFN) cloned with the β-interferon gene alone. Way. 제 1 항에 있어서, 인공합성링커는 SOD(Superoxide Dismutase) 유전자의 앞부분 6개의 아미노산 DNA배열과 종료코돈 TAA로 연결된 염기배열 순서로 된 것임을 특징으로하는 인간 β-인터페론 의 제조 방법.The method of claim 1, wherein the artificial linker is a sequence consisting of a sequence consisting of a six-amino acid DNA sequence of the front part of the SOD (Superoxide Dismutase) gene and an end codon TAA. 제 1 항 또는 제 2 항에 있어서, 인공합성 링커를의 유전자의 5번째 아미노산인 알라닌을 만드는 코돈인 GCC를 GAA로 바꾸어 SD배열인 AGGA염기배열을 만드는 것임을 특징으로 하는 인간 β-인터페론 의 제조방법.The method for producing human β-interferon according to claim 1 or 2, wherein an artificial synthetic linker converts GCC, which is a codon for making alanine, the fifth amino acid of the gene, into GAA, to make AGGA base sequence of SD sequence. . 제 1 항에 있어서, 촤종 대장균 발현 운반체 β-인터페론 링커 및 tac-프로모터가 결합된 운반체(ptac5IS3 IFN)임을 특징으로하는 인간 β-인터페론 의 제조 방법.The method for producing human β-interferon according to claim 1, which is a carrier (ptac5IS3 IFN) in which an E. coli expression carrier β-interferon linker and a tac-promoter are combined. 제 1 항에 있어서, 유도 발현은 β-인터페론이 함유된 대장균을 흡광도(650nm) 1.5-2.에서 IPTG(Isopropyl thiogalactoside)의 최종 농도가 2mM이 되도록 처리한 후 3시간 동안 배양시킴을 특징으로 하는 인간 β-인터페론의 제조방법.The method of claim 1, wherein the induced expression is characterized by incubating for 3 hours after treatment with β-interferon-containing E. coli so that the final concentration of IPTG (Isopropyl thiogalactoside) at absorbance (650nm) 1.5-2. Method for preparing human β-interferon. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019860003834A 1986-05-14 1986-05-14 Process for mass production of human-beta interferon KR900007868B1 (en)

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KR1019860003834A KR900007868B1 (en) 1986-05-14 1986-05-14 Process for mass production of human-beta interferon

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Application Number Priority Date Filing Date Title
KR1019860003834A KR900007868B1 (en) 1986-05-14 1986-05-14 Process for mass production of human-beta interferon

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KR870011248A true KR870011248A (en) 1987-12-22
KR900007868B1 KR900007868B1 (en) 1990-10-22

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