KR850003106A - Seed Modification Using Microorganisms to Promote Root Development - Google Patents

Seed Modification Using Microorganisms to Promote Root Development Download PDF

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KR850003106A
KR850003106A KR1019840006658A KR840006658A KR850003106A KR 850003106 A KR850003106 A KR 850003106A KR 1019840006658 A KR1019840006658 A KR 1019840006658A KR 840006658 A KR840006658 A KR 840006658A KR 850003106 A KR850003106 A KR 850003106A
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agrobacterium
seed
risogenes
seeds
separation tank
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KR1019840006658A
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Korean (ko)
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모리스 앤더슨 데이비드
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토드 테일러
피토 겐
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Publication of KR850003106A publication Critical patent/KR850003106A/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06—Coating or dressing seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Soil Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Agronomy & Crop Science (AREA)
  • Medicinal Chemistry (AREA)
  • Dentistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Pest Control & Pesticides (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

내용 없음No content

Description

뿌리발육증진에 있어서 미생물을 이용한 종자변형방법Seed Modification Using Microorganisms to Promote Root Development

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (16)

종자를 심기전에 뿌리를 내리도록 아그로신(agrocin)을 자극하는아그로박테리움 리조게넥스가 종자를 변형시킬 수 있는 변형작용을 할 수 있도록 충분한 시간동안 종자를 노출시키고, 변형된 종자의 부위에 남은아그로박테리움 리조게네스를 포함할 수 있는 충분한 양의아그로박테리움 래디어박터를아그로박테리움 리조게네스와 함께 사용하여 쌍자엽 식물의 종자를 유전적으로 변형시켜 발아시에 뿌리발육을 증진시키는 것을 특징으로 하는 종자변형 방법.Expose the seeds for a sufficient time so that agrobacterium lyogenene, which stimulates agrocin to take root before planting the seeds, can act to modify the seeds, leaving them at the site of the modified seeds. characterized in that Agrobacterium separation tank to a sufficient amount of the Agrobacterium below media bakteo which may include a harness Agrobacterium separation tank to use with a harness by modifying the seeds of ssangjayeop plants genetically to promote root development during germination Seed modification method. 제1항에 있어서,아그로박테리움 리조게네스는아그로박테리움 리조게네스TR 105를 포함하는 것을 특징으로 하는 방법.The method of claim 1, wherein the Agrobacterium risogenes comprises Agrobacterium risogenes TR 105. 제 1항에 있어서,아그로박테리움 리조게네스는아그로박테리움 리조게네스ATCC 39207을 포함하는 것을 특징으로 하는 방법.The method of claim 1, wherein the Agrobacterium risogenes comprises Agrobacterium risogenes ATCC 39207. 전항 중 어느한 항에 있어서,아그로박테리움 래디오박터는아그로박테리움 래디오박터K-84를 포함하는 것을 특징으로 하는 방법.The method of claim 1, wherein the Agrobacterium radiobacter comprises Agrobacterium radiobacter K-84. 전항 중 어느한 항에 있어서,아그로박테리움 리조게네스는 종자에 유착되는 것을 특징으로 하는 방법.The method of claim 1, wherein the Agrobacterium risogenes is adhered to the seed. 전항 중 어느한 항에 있어서,아그로박테리움 리조게네스는 이탄속에 포함되어 있는 것을 특징으로 하는 방법The method of claim 1, wherein the Agrobacterium risogenes is included in peat. 전항 중 어느한 항에 있어서,아그로박테리움 리조게네스와아그로박테리움 레디오 박터는 이탄에 있는 종자속에 유착 되어있는 것을 특징으로 하는 방법.The method of any one of the preceding claims, wherein Agrobacterium risogenes and Agrobacterium radiobacter are adhered to the seed in peat. 전항 중 어느한항에 있어서, 뿌리를 내리게 하는아그로박테리움 리조게네스는 뿌리발육을 증진시키기 위해 유전적으로 종자를 변형시키는데 있어서 수성의 현탁액속에 ml당 108세포수의 농도를 존재하는 것을 특징으로 하는 방법.The rooted Agrobacterium risogenes is characterized by the presence of a concentration of 10 8 cells per ml in an aqueous suspension in genetically modifying seeds to enhance root development. How to. 전항 중 어느한 항에 있어서,아그로박테리움 래디오터는아그로박테리움 리조게네스 대(對)아그로박테리움 래디오박터의 세포비가 4대 1인 농도를 제공되는 것을 특징으로하는 방법.According to any one of the preceding paragraph, the radio and Agrobacterium emitter is characterized in that the Agrobacterium separation tank to provide a harness for (對) Agrobacterium cells, the radio and bakteo ratio 4 of the first concentration. 아그로 박테리움 리조게네스가 종자를 노출시켜 변형시키는데 있어서 이 미생물은 수성의 현탁액농도와 똑 같은 물 ml당 108세포수를 가지며아그로 박테리움 리조게네스대아그로박테리움 래디오박터의세포비가 4대 1인 농도로 배지내에 존재하며, 배지내에는 뿌리를 내리게하는 상기 두미생물의 혼합물이 있어서 이것에 쌍자엽식물의 종자를 노출시켜 유전적으로 종자를 변형시키는 것을 특징으로하는 방법. Agrobacterium separation tank to Ness, this microorganism according to transform by exposing the seed has the 10 same water per ml and the suspension concentration of the aqueous 8 cells Agrobacterium separation tank to Ness for Agrobacterium cells tumefaciens the radio and bakteo ratio 4 And a mixture of the two microorganisms present in the medium at a concentration of 1, wherein the medium is rooted, thereby exposing the seed of the dicotyledonous plant to genetically modify the seed. 전항 중 어느한 항에 있어서,아그로박테리움 리조게네스와아그로박테리움 래디오박터는 세포비가 4대 1 내지 8대 1의 비로 존재하는 것을 특징으로하는 방법.The method of any one of the preceding claims, wherein the Agrobacterium risogenes and the Agrobacterium radiobacter are present in a ratio of 4 to 1 to 8 to 1. 아그로박테리움 리조게네스에 의해 종자를 변형시킬 수 있는 배지내에서아그로박테리움 마조게네스TR-105,아그로박테리움 리조게네스ATCC39207 및 그혼합물로부터 선택된아그로박테리움 리조게네스의 변형작용에 의하여 종자를 노출시켜 유전적으로 종자를 변형시키는 것을 특징으로 하는 방법. By the action of Agrobacterium risogenes selected from Agrobacterium mazogenes TR-105, Agrobacterium risogenes ATCC39207 and mixtures thereof in a medium capable of modifying the seed by Agrobacterium risogenes Exposing the seed to genetically modify the seed. 제12항에 있어서, 배지는 이탄인 것을 특징으로 하는 방법.13. The method of claim 12, wherein the medium is peat. 전항 중 어느한 항에 있어서, 종자는 콩이거나 또는 목화인 것을 특징으로 하는 방법.The method of claim 1, wherein the seed is bean or cotton. 전항 중 어느 한 항에 의해 생성되는 것을 특징으로 하는 변형된 종자.Modified seed, characterized in that produced by any one of the preceding. 제15항에 있어서, 변형된 종자로부터 성장되는 것을 특징으로 하는 식물.The plant of claim 15, wherein the plant is grown from modified seeds. ※참고사항:최초출원 내용에 의하여 공개하는 것임.※ Note: This is to be disclosed based on the first application.
KR1019840006658A 1983-10-26 1984-10-26 Seed Modification Using Microorganisms to Promote Root Development KR850003106A (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US54575683A 1983-10-26 1983-10-26
US545756 1983-10-26
US64586084A 1984-08-31 1984-08-31
US645860 1984-08-31

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KR850003106A true KR850003106A (en) 1985-06-13

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KR (1) KR850003106A (en)
ES (1) ES8602355A1 (en)
FR (1) FR2560744A1 (en)
GR (1) GR80695B (en)
IL (1) IL73242A0 (en)
IT (1) IT1179148B (en)
PL (1) PL250162A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4588693A (en) * 1983-02-28 1986-05-13 Research And Development Institute, Inc. At Montana State University Development of plant roots
TR22317A (en) * 1984-04-03 1987-02-04 Univ Montana Res Dev Inst PROCEDURE AND COMPUTER FOR THE DEVELOPMENT OF PLANT KOEKS FOR THE USE OF PLANT KOEKS IN A BENEFIT FORM.
US5015580A (en) * 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
EP0309551B1 (en) * 1987-04-03 1993-05-19 Kamterter Products, Inc. Solid matrix priming with microorganisms and chemicals
WO1989005859A1 (en) * 1987-12-21 1989-06-29 The Upjohn Company Agrobacterium mediated transformation of germinating plant seeds
EP1348325A3 (en) * 1997-11-26 2004-02-25 Kamterter II, L.L.C. Solid matrix conditioning of seeds

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4425150A (en) * 1981-05-04 1984-01-10 Research And Development Institute, Inc. At Montana State University Compositions containing and methods of use of an infectivity-cured Hr plasmid-bearing microorganism
US4588693A (en) * 1983-02-28 1986-05-13 Research And Development Institute, Inc. At Montana State University Development of plant roots

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GR80695B (en) 1985-02-13
IT1179148B (en) 1987-09-16
IT8468060A0 (en) 1984-10-25
IL73242A0 (en) 1985-01-31
PL250162A1 (en) 1985-07-16
ES537056A0 (en) 1985-12-01
IT8468060A1 (en) 1986-04-25
FR2560744A1 (en) 1985-09-13
ES8602355A1 (en) 1985-12-01

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