KR850001803A - Method of Making Peptides - Google Patents

Method of Making Peptides Download PDF

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Publication number
KR850001803A
KR850001803A KR1019830003916A KR830003916A KR850001803A KR 850001803 A KR850001803 A KR 850001803A KR 1019830003916 A KR1019830003916 A KR 1019830003916A KR 830003916 A KR830003916 A KR 830003916A KR 850001803 A KR850001803 A KR 850001803A
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KR
South Korea
Prior art keywords
peptide
culture
iaa
microorganism
promoter
Prior art date
Application number
KR1019830003916A
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Korean (ko)
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KR900004942B1 (en
Inventor
타미오(외 3) 미주카미
Original Assignee
키노시타 수쿠오
쿄와 하꼬 고오고 가부시끼 가이샤
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Priority to KR1019830003916A priority Critical patent/KR900004942B1/en
Publication of KR850001803A publication Critical patent/KR850001803A/en
Application granted granted Critical
Publication of KR900004942B1 publication Critical patent/KR900004942B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음No content

Description

펜티드(peptide)의 제조방법Manufacturing method of pentide

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (5)

진핵세포성 펩티드를 코드화하는 유전자, 인자(factor) 및 프로모우터(promotor)로 되는 조환 DNA를 함유하고, 또한 이 펩티드 생산능력을 소유한 미생물을 배지에 배양하여, 배양물중에 이 펩티드를 축적시키기 위하여, 이 배양물에서 펩티드를 채취할 때에, 미생물의 적합한 생육온도보다 10 내지 25℃ 낮은 온도에서 배양시키는 것을 특징으로 하는 펩티드의 제조방법.A microorganism containing the DNA, which is a gene, a factor, and a promoter encoding a eukaryotic peptide, and which possesses the peptide production capacity is cultured in a medium to accumulate the peptide in culture. In order to obtain a peptide from the culture, the method for producing a peptide, characterized in that the culture is carried out at a temperature of 10 to 25 ℃ lower than the suitable growth temperature of the microorganism. 제1항에 있어서, 프로모우터가 트립토판 프로모우터인 방법.The method of claim 1, wherein the promoter is a tryptophan promoter. 제1항 또는 제2항에 있어서, 미생물의 배양중 대수증식기의 후반부터 최고증식을 나타낼때까지 배지에 3-인돌 아크릴산(3-indole acryic acid)(이하 IAA로 약기한다)을 가하는 것을 특징으로 하는 방법.The method according to claim 1 or 2, wherein 3-indole acryic acid (hereinafter abbreviated as IAA) is added to the medium from the late stage of the logarithmic growth stage to the highest growth in the culture of the microorganism. How to. 제1항, 2항 및 3항중 어느 하나에 있어서, IAA 첨가후, IAA를 연속적 또는 간헐적으로 첨가하는 것을 특징으로 하는 방법.The method according to any one of claims 1, 2 and 3, wherein after adding IAA, IAA is added continuously or intermittently. 제1항, 2항 및 3항 및 4항중 어느 하나에 있어서, 펩티드가 인체 β형 인터-페론의 펩티드로 되는 것을 특징으로 하는 방법.The method according to any one of claims 1, 2 and 3 and 4, wherein the peptide is a peptide of human β-type inter-feron. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019830003916A 1983-08-22 1983-08-22 Process for peparing peptides KR900004942B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019830003916A KR900004942B1 (en) 1983-08-22 1983-08-22 Process for peparing peptides

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019830003916A KR900004942B1 (en) 1983-08-22 1983-08-22 Process for peparing peptides

Publications (2)

Publication Number Publication Date
KR850001803A true KR850001803A (en) 1985-04-01
KR900004942B1 KR900004942B1 (en) 1990-07-12

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ID=19229757

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019830003916A KR900004942B1 (en) 1983-08-22 1983-08-22 Process for peparing peptides

Country Status (1)

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KR (1) KR900004942B1 (en)

Also Published As

Publication number Publication date
KR900004942B1 (en) 1990-07-12

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