KR830005347A - Microbiologically prepared polypeptides having amino acid sequences of proinsulins of primates, DNAs and plasmids coding for microorganisms containing amino acid sequences and genetic information and methods for preparing the same - Google Patents

Microbiologically prepared polypeptides having amino acid sequences of proinsulins of primates, DNAs and plasmids coding for microorganisms containing amino acid sequences and genetic information and methods for preparing the same Download PDF

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Publication number
KR830005347A
KR830005347A KR1019810000097A KR810000097A KR830005347A KR 830005347 A KR830005347 A KR 830005347A KR 1019810000097 A KR1019810000097 A KR 1019810000097A KR 810000097 A KR810000097 A KR 810000097A KR 830005347 A KR830005347 A KR 830005347A
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South Korea
Prior art keywords
amino acid
acid sequences
primates
genetic information
proinsulins
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KR1019810000097A
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Korean (ko)
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벤겐마이어 프리드리히
그로네버그 위르겐
한스-디이터줌
빈나커 에른스트-루드비히
Original Assignee
베른하르트 벡크, 하인리히 벡커
훽스트 아크티엔 게젤샤프트
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Application filed by 베른하르트 벡크, 하인리히 벡커, 훽스트 아크티엔 게젤샤프트 filed Critical 베른하르트 벡크, 하인리히 벡커
Priority to KR1019810000097A priority Critical patent/KR830005347A/en
Publication of KR830005347A publication Critical patent/KR830005347A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
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  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음No content

Description

영장류의 프로인슈린의 아미노산 서열을 가진 미생물학적으로 제조된 폴리펩타이드, 아미노산 서열, 유전정보가 함유된 미생물에 코딩으로 작용하는 DNA 및 플라스미드와 이의 제조방법Microbiologically prepared polypeptides having amino acid sequences of proinsulins of primates, DNAs and plasmids coding for microorganisms containing amino acid sequences and genetic information and methods for preparing the same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (4)

영장류의 프로인슈린의 아미노산 서열을 가진 폴리펩타이드의 생합성에 사용되는 유전정보를 전부 또는 일부 함유한 미생물을 배양시키고 미생물에서 형성된 폴리펩타이드를 분리시킴을 특징으로 하는 폴리펩타이드의 제조방법.A method for producing a polypeptide, comprising culturing a microorganism containing all or part of genetic information used for biosynthesis of a polypeptide having an amino acid sequence of proinsulin of a primate, and separating the polypeptide formed from the microorganism. 영장류의 췌장에서 RNA를 수득하고 여기에서 mRNA를 분리시킨후 이 mRNA를 사용하여 cDNA를 제조함을 특징으로하는 영장류의 프로인슈민의 아미노산 서열에 코딩으로 작용하는 cDNA의 제조방법.A method for producing cDNA, which acts as a coding for the amino acid sequence of proinsulin in primates, characterized by obtaining RNA from the pancreas of primates and isolating mRNAs therefrom to produce cDNAs. 상기 특허청구의 범위 2의 방법에 따라 제조된 cDNA를 플라스미드, 바람직하기는 플라스미드 pBR322와 결합시킴을 특징으로하여 영장류의 프로인슈린의 아미노산 서열에 코딩으로 작용하는 플라스미드를 제조하는 방법.CDNA prepared according to the method of claim 2 to a plasmid, preferably plasmid pBR322, characterized in that to bind to the amino acid sequence of the proinsulin of primates to prepare a plasmid. 수용성의 진한 염화세슘 용액내의 층상에 염화세슘이 유리된 융해질을 가하고 원심분리시켜 RNA를 수득함을 특징으로하는 RNA의 제조방법.A process for producing RNA, characterized in that the cesium chloride-free fusion is added to a layer in an aqueous concentrated cesium chloride solution and centrifuged to obtain RNA. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019810000097A 1981-01-15 1981-01-15 Microbiologically prepared polypeptides having amino acid sequences of proinsulins of primates, DNAs and plasmids coding for microorganisms containing amino acid sequences and genetic information and methods for preparing the same KR830005347A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019810000097A KR830005347A (en) 1981-01-15 1981-01-15 Microbiologically prepared polypeptides having amino acid sequences of proinsulins of primates, DNAs and plasmids coding for microorganisms containing amino acid sequences and genetic information and methods for preparing the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019810000097A KR830005347A (en) 1981-01-15 1981-01-15 Microbiologically prepared polypeptides having amino acid sequences of proinsulins of primates, DNAs and plasmids coding for microorganisms containing amino acid sequences and genetic information and methods for preparing the same

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KR830005347A true KR830005347A (en) 1983-08-13

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KR1019810000097A KR830005347A (en) 1981-01-15 1981-01-15 Microbiologically prepared polypeptides having amino acid sequences of proinsulins of primates, DNAs and plasmids coding for microorganisms containing amino acid sequences and genetic information and methods for preparing the same

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