KR790001609B1 - Simultaneous process for the preparation of xylose and ethanol from materials containing cellulose - Google Patents

Abstract

Xylose and ethanol were prepd. simultaneously by culturing Trichoderma viride Qm 9123 which produced cellulase and hemicellulase, and Saccharomyces cerlisiae NCYC 478R producing EtOH with high yield, at agricultural wastes as rice straws contg. cellulose and hemicallulose as main components to produce xylose as pentose and hexose which was fermented to give ethanol.

Description

Simultaneous preparation method of gyrose and ethyl alcohol from cellulose-containing materials

It is a feature of the present invention to simultaneously produce microorganism culture containing cellulose or alcohol fermentation bacteria with agricultural waste resources or plants, such as cereal straws of cellulose and hemicellulose, and to produce gyrose and ethyl alcohol simultaneously. to be.

Plants containing cellulose and hemicellulose are described in detail to produce gyros and ethyl alcohol as raw materials.

To date, known methods have produced ethanol, which is a major component of glucose and hemicellulose, produced by decomposing plants by enzymes or chemical methods, or by fermenting the sugar solution with secondary fermentation to produce ethyl alcohol. When the cellulose is decomposed by the enzymatic method to produce ethyl alcohol by the conventional method, the produced glucose inhibits the activity of the cellulase, making it difficult to produce a high concentration of sugar solution in a short time. In addition, when gyros are produced by enzymatic or chemical methods using plant materials, plant constituent sugars, such as glucose and garactose, are mixed and have difficulty in separating and purifying them.

By using the ethyl alcohol producing bacteria at the same time while decomposing the plant by a microbial enzyme containing the cellulase and hemicellulase simultaneously in the method of the present invention can solve the problems occurring in the conventional method described above. That is, hexasaccharide such as glucose produced by enzyme action is fermented with alcohol to remove the inhibitory factor of enzyme activity, and gyro, which is pentose 5, can be produced in high purity because it is not fermented by ethyl alcohol fermentation bacteria.

The main constituents of agricultural wastes such as cereals and straws are cellulose rose hemicellulose and lignin. Among them, lignin is very slow in decomposition, but polysaccharides such as cellulose and hemicellulose are relatively easily broken down. Hydrolysis can produce glucose in cellulose, while hemicellulose mainly produces hexalose, a small amount of galactose, hexasaccharides such as glucose and pentose sugars such as arabinose. In general, microorganisms producing cellulase, a cellulose degrading enzyme, produce hemicellulase at the same time, so when enzymes produced by these microorganisms are used to break down plants such as agricultural waste resources, they occur in glucose and hemicellulose derived from cellulose. A mixed solution such as gyro galactose is obtained. Saccharomyces cerevisiae, an ethyl alcohol fermentation bacterium, ferments glucose and galactose, hexasaccharide, to produce alcohol, but gyro, which is 5-saccharide, cannot ferment. Trichoderma is a substrate of corn cob with high hemicellulose content and straw of textiles. Liquid immersion media or solid cultures of cellulose degrading bacteria such as Trichoderma viride, Aspergillus, Aspergillus niger, Apex, and Irpex lacteus, as well as solid cultures and Saccharomyces, It can be seen in the examples that the fermentation of ethyl alcohol fermentation bacteria, such as cerevisiae) at the same time to obtain a high-purity gyrose and ethyl alcohol. In addition, it is possible to use a microorganism that does not magnetize for the purpose of purifying gyros by removing hexose from the sugar solution containing gyros.

Example 1

Trichoderma, viridide QM9123 (Trichoderma viride QM9123) or KI 7-2 fungus isolated from soil, which has strong cellulase and hemicellulase production, is a bran solid medium (20g of bran mixed with 20ml of water Incubated for 7 days at 26 ° C. in sterilized at 121 ° C. for 24 minutes, and separately in glucose-malt medium (0.75 g of malt extract and 0.5 g of glucose in 50 ml of distilled water adjusted to pH 4.5) for 3 days. Saccharomyces cerevisiae NCYC 478 R yeast bacteria were prepared by shaking culture at ℃. 50 g of corn cob powder (40 mesh) was suspended in 300 ml of water, 0.3 g of KH ₂ PO ₄ was added, and the solution was autoclaved at 121 ° C. for 15 minutes by adjusting the pH to 4.0. The trichoderma solid culture and yeast bacteria prepared in advance were mixed well with corncob suspension and allowed to stand at 26 ° C for 5 days. The resulting ethyl alcohol and reducing sugars were prepared using the AO AC 11.006 method and the Somongi-Nelson method. Each measurement was carried out and the results of Table 1 were obtained. At this time, the control group without adding corncob meal was analyzed in the same manner.

Separation of the reducing sugars in the fermentation broth revealed no presence of hexasaccharides (0.5 g / l or less), and the conversion source was gyrose except for a small amount of arabinose (5% w / w or less of the total reducing sugars). .

[Table 1] Production of gyro and ethyl alcohol from corn cobs

Example 2

Rice straw was pulverized with a ball mill for 24 hours to make powder, and 50 g of the straw was fermented in the same manner as in Example 1 to obtain the results shown in Table 2. The reduction in fermentation broth was analyzed by thin layer chromatography and found to be 95% w / w or more of gyro.

[Table 2] Production of gyro and ethyl alcohol from rice straw

Example 3

Rice straw was made into powder in the same manner as in Example 2, and 50 g of the powder was immersed in 150 ml of a prescribed concentration of caustic soda solution at room temperature for 5 hours, and then pH was adjusted to 4.0 with 5 prescribed concentrations of hydrochloric acid. After filling the liquid amount with 300 ml of 0.3 g of KH ₂ PO ₄ dissolved in the treated straw straw turbidity and fermented in the same manner as in Example 1 to obtain the results of Table 3.

Table 3 Production of Gyrose and Ethyl Alcohol from Alkaline Treated Rice Straw

Example 4

50 g of cellulose powder, Solka Floc, was treated in the same manner as in Example 1, and 3.2% v / v of ethyl alcohol in the fermentation broth was treated with Tricoderma and Viride QM9123, and Tricoderma, genus KI 7 The spheres with -2 were able to produce 3.8% v / v of alcohol.

Claims (1)

It is characterized by fermenting hexasaccharides produced by ethyl alcohol with a culture of Trichoderma filamentous fungi containing cellulase and hemicellulase and fungi with alcohol fermentation ability to cellulose and hemicellulose containing materials. Method for producing a high-purity gyro and ethyl alcohol at the same time.