KR20240050749A - Method of estimating digestible energy and metabolic energy using Hermetia illucens raw feed nutrient analysis - Google Patents
Method of estimating digestible energy and metabolic energy using Hermetia illucens raw feed nutrient analysis Download PDFInfo
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- 241000709785 Hermetia illucens Species 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 29
- 230000002503 metabolic effect Effects 0.000 title abstract description 14
- 238000004458 analytical method Methods 0.000 title abstract description 7
- 235000015097 nutrients Nutrition 0.000 title abstract description 7
- 241001481656 Stratiomyidae Species 0.000 claims abstract description 30
- 235000019750 Crude protein Nutrition 0.000 claims abstract description 28
- 235000019784 crude fat Nutrition 0.000 claims abstract description 27
- 239000004615 ingredient Substances 0.000 claims description 14
- 238000004364 calculation method Methods 0.000 claims description 4
- 238000009313 farming Methods 0.000 claims description 4
- 239000000835 fiber Substances 0.000 abstract description 6
- 239000002253 acid Substances 0.000 abstract description 5
- 238000010171 animal model Methods 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 description 11
- 239000000126 substance Substances 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 210000002700 urine Anatomy 0.000 description 5
- 210000003608 fece Anatomy 0.000 description 4
- 241000282887 Suidae Species 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 235000019197 fats Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000019733 Fish meal Nutrition 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 230000002550 fecal effect Effects 0.000 description 2
- 239000004467 fishmeal Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000021049 nutrient content Nutrition 0.000 description 2
- 239000004455 soybean meal Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- OBMBUODDCOAJQP-UHFFFAOYSA-N 2-chloro-4-phenylquinoline Chemical compound C=12C=CC=CC2=NC(Cl)=CC=1C1=CC=CC=C1 OBMBUODDCOAJQP-UHFFFAOYSA-N 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108010060231 Insect Proteins Proteins 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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Abstract
본 발명은, 동애등에 원료사료 영양소 분석을 이용한 가소화에너지 및 대사에너지 추정 방법에 관한 것으로서, 본 발명은 동애등에 내 조단백질, 조지방, 조회분 및 산성세제불용성섬유소 함량을 확인하여, 동애등에 원료사료의 총에너지 함량을 추정할 수 있는 방법을 도출하였다. 또한, 동애등에를 원료사료를 동물모델에 급여한 후, 동애등에 원료사료의 가소화에너지 및 대사에너지 함량을 확인하였으며, 이를 통해 통계적으로 유의한 동애등에 원료사료의 가소화에너지 및 대사에너지 추정 방법을 도출하였다.The present invention relates to a method for estimating digestible energy and metabolic energy using nutrient analysis of raw feed for black soldier flies, etc. The present invention relates to a method for estimating crude protein, crude fat, ash, and acid detergent-insoluble fiber content in black soldier flies, etc., and providing raw feed for black soldier flies, etc. A method to estimate the total energy content of was derived. In addition, after feeding black soldier fly raw feed to an animal model, the digestible energy and metabolic energy content of the raw black soldier feed were confirmed, and through this, a statistically significant method of estimating the digestible energy and metabolic energy of the raw black soldier feed was found. was derived.
Description
본 발명은, 동애등에 원료사료 영양소 분석을 이용한 가소화에너지 및 대사에너지 추정 방법에 관한 것이다.The present invention relates to a method for estimating digestible energy and metabolic energy using nutrient analysis of raw feed for black soldier flies.
양돈사료 내 충분한 에너지 공급은 돼지의 성장에 중요하다. 에너지 공급원인 원료사료는 사료비에서 가장 큰 부분을 차지하며, 주요 원료사료는 옥수수 및 대두박과 같은 식물성 원료사료가 있고 어분 및 혈장 단백질과 같은 동물성 원료사료가 있다. 하지만 양돈사료에서 사용되는 대부분 원료사료는 수입에 의존하고 있어 원료가격이 일정하지 않으며, 옥수수와 대두박 같은 식물성 원료는 국제 곡물 가격에 영향을 받고, 어분과 같은 수입 단백질 공급원은, 기후 변화로 인한 수산자원량 감소에 의해 원료가격이 영향을 받을 수 있다. 이에 곤충 단백질 원료사료인 동애등에는 국내 생산에 기반을 두고 있으며, 조단백질 및 조지방 함량이 높아 다른 원료사료를 대체할 만한 에너지 원료사료로 각광받고 있다. 하지만, 양돈 체내에서의 동애등에 원료사료 에너지 이용성에 관한 연구는 부족한 실정이다(Crosbie et al., Translational Animal Science, Volume 4, Issue 3, July 2020, txaa104).Sufficient energy supply in pig feed is important for pig growth. Raw feed, which is a source of energy, accounts for the largest portion of feed costs. The main raw feeds include vegetable raw feed such as corn and soybean meal, and animal raw feed such as fish meal and plasma protein. However, most of the raw materials used in pig feed depend on imports, so raw material prices are not constant, vegetable raw materials such as corn and soybean meal are affected by international grain prices, and imported protein sources such as fishmeal are subject to fisheries problems due to climate change. Raw material prices may be affected by a decrease in resources. Accordingly, Dongae, an insect protein raw feed, is based on domestic production, and is attracting attention as an energy raw feed that can replace other raw feeds due to its high crude protein and crude fat content. However, there is a lack of research on the energy utilization of raw feed by black soldier flies in pig farming (Crosbie et al., Translational Animal Science, Volume 4, Issue 3, July 2020, txaa104).
동애등에는 먹이, 치사방법, 건조방법, 지방추출방법 및 기타가공과정에 의해 생체 내 화학적 조성이 다양하게 형성된다(Barragan-Fonseca et al., Journal of Insects as Food and Feed, Volume 3, Number 2, 16 June 2017, pp. 105-120(16)). 특히, 동애등에의 화학적 조성은 지방추출 정도에 따라 큰 영향을 받는데, 지방추출 정도에 따라 지방 함량은 약 5% 내지 30%, 조단백질 함량은 약 30% 내지 50%로 변이가 큰 편이다. 결국, 다양한 동애등에의 화학적 조성은 에너지 이용성에 영향을 미칠 뿐만 아니라 동애등에의 총에너지, 가소화에너지 및 대사에너지 함량에도 영향을 미칠 수 있다. The chemical composition in the body of black soldier flies is formed in various ways depending on the food, killing method, drying method, fat extraction method, and other processing processes (Barragan-Fonseca et al., Journal of Insects as Food and Feed, Volume 3, Number 2 , 16 June 2017, pp. 105-120(16). In particular, the chemical composition of black soldier flies is greatly affected by the degree of fat extraction. Depending on the degree of fat extraction, the fat content is about 5% to 30% and the crude protein content is about 30% to 50%, which varies greatly. Ultimately, the chemical composition of various black soldier flies not only affects energy availability, but can also affect the total energy, digestible energy, and metabolizable energy contents of black soldier flies.
이에 본 발명자들은 동애등에 원료사료 내 영양소 분석치를 기반으로 다양한 동애등에 원료사료 내 총에너지, 가소화에너지 및 대사에너지 함량을 예측할 수 있는 추정 방법을 이용하여, 본 발명을 완성하였다.Accordingly, the present inventors completed the present invention by using an estimation method that can predict the total energy, digestible energy, and metabolic energy content in various black soldier fly raw feed based on the nutrient analysis values in the raw black soldier feed.
본 발명의 목적은 동애등에(Hermetia illucens)의 조단백질, 조지방 또는 조회분 함량(%)을 측정하는 단계; 및The object of the present invention is to measure the crude protein, crude fat or ash content (%) of black soldier fly ( Hermetia illucens ); and
상기 측정된 조단백질, 조지방 또는 조회분 함량으로부터, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량 측정 방법을 제공하는 것이다.From the measured crude protein, crude fat, or ash content, a method of measuring the total energy, digestible energy, or metabolizable energy content of feed ingredients for Dongae, etc. is provided.
본 발명의 다른 목적은 동애등에(Hermetia illucens)의 조단백질, 조지방 또는 조회분 함량(%) 측정부; 및Another object of the present invention is to measure the crude protein, crude fat or ash content (%) of black soldier fly ( Hermetia illucens ); and
상기 측정된 조단백질, 조지방 또는 조회분 함량으로부터, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량을 연산하는 연산부를 포함하는, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량 측정 장치를 제공하는 것이다.Total energy of feed ingredients for black soldier flies, etc., including a calculation unit that calculates the total energy, digestible energy, or metabolizable energy content of the feed ingredients for black soldier flies, etc., from the measured crude protein, crude fat, or ash content. , to provide a device for measuring digestible energy or metabolizable energy content.
상기 목적을 달성하기 위하여, 본 발명은 동애등에(Hermetia illucens)의 조단백질, 조지방 또는 조회분 함량(%)을 측정하는 단계; 및In order to achieve the above object, the present invention includes the steps of measuring the crude protein, crude fat or ash content (%) of black soldier fly ( Hermetia illucens ); and
상기 측정된 조단백질, 조지방 또는 조회분 함량으로부터, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량 측정 방법을 제공한다.From the measured crude protein, crude fat or ash content, a method for measuring the total energy, digestible energy or metabolizable energy content of feed ingredients for Dongae, etc. is provided.
또한, 본 발명은 동애등에(Hermetia illucens)의 조단백질, 조지방 또는 조회분 함량(%) 측정부; 및In addition, the present invention provides a measuring unit for crude protein, crude fat or ash content (%) of black soldier fly ( Hermetia illucens ); and
상기 측정된 조단백질, 조지방 또는 조회분 함량으로부터, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량을 연산하는 연산부를 포함하는, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량 측정 장치를 제공한다.Total energy of feed ingredients for black soldier flies, etc., including a calculation unit that calculates the total energy, digestible energy, or metabolizable energy content of the feed ingredients for black soldier flies, etc., from the measured crude protein, crude fat, or ash content. , Provides a device for measuring digestible energy or metabolizable energy content.
본 발명은 동애등에 내 조단백질, 조지방, 조회분 및 산성세제불용성섬유소 함량을 확인하여, 동애등에 원료사료의 총에너지 함량을 추정하는 방법을 도출하였다. 또한, 동애등에 원료사료를 동물모델에 급여한 후, 동애등에 원료사료의 가소화에너지 및 대사에너지 함량을 확인하였으며, 이를 통해 통계적으로 유의한 동애등에 원료사료의 가소화에너지 및 대사에너지 추정 방법을 도출하여, 관련 산업에 유용하게 이용할 수 있다.The present invention identified the crude protein, crude fat, ash, and acid detergent-insoluble fiber content in black soldier flies, and derived a method for estimating the total energy content of raw feed for black soldier flies. In addition, after feeding black soldier fly raw feed to an animal model, the digestible energy and metabolic energy contents of the raw black soldier feed were confirmed, and through this, a statistically significant method of estimating the digestible energy and metabolic energy of the raw black soldier feed was established. It can be derived and usefully used in related industries.
이하 본 발명의 실시예들을 상세히 설명한다. 이하의 설명에 있어, 당업자에게 주지 저명한 기술에 대해서는 그 상세한 설명을 생략할 수 있다. 또한, 본 발명을 설명함에 있어서, 관련된 공지 기능 또는 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명을 생략할 수 있다. 또한, 본 명세서에서 사용되는 용어(terminology)들은 본 발명의 바람직한 실시예를 적절히 표현하기 위해 사용된 용어들로서, 이는 사용자, 운용자의 의도 또는 본 발명이 속하는 분야의 관례 등에 따라 달라질 수 있다.Hereinafter, embodiments of the present invention will be described in detail. In the following description, detailed descriptions of techniques well known to those skilled in the art may be omitted. Additionally, when describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description may be omitted. In addition, the terminology used in this specification is a term used to appropriately express preferred embodiments of the present invention, and may vary depending on the intention of the user or operator or the customs of the field to which the present invention belongs.
따라서 본 용어들에 대한 정의는 본 명세서 전반에 걸친 내용을 토대로 내려져야 할 것이다. 명세서 전체에서, 어떤 부분이 어떤 구성요소를 "포함"한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성요소를 제외하는 것이 아니라 다른 구성 요소를 더 포함할 수 있는 것을 의미한다.Therefore, definitions of these terms should be made based on the content throughout this specification. Throughout the specification, when a part is said to “include” a certain element, this means that it may further include other elements rather than excluding other elements, unless specifically stated to the contrary.
본 발명은 동애등에(Hermetia illucens)의 조단백질, 조지방 또는 조회분 함량(%)을 측정하는 단계; 및The present invention includes the steps of measuring the crude protein, crude fat or ash content (%) of black soldier fly ( Hermetia illucens ); and
상기 측정된 조단백질, 조지방 또는 조회분 함량으로부터, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량 측정 방법을 제공한다.From the measured crude protein, crude fat or ash content, a method for measuring the total energy, digestible energy or metabolizable energy content of feed ingredients for Dongae, etc. is provided.
본 발명의 "사료"란 동물에 의해 소비되고 동물의 규정식에 에너지 및/또는 영양소를 기여하는 재료(들)를 지칭한다. 사료의 예는, 비제한적으로, 완전 배합 사료(TMR), 여물(들), 펠렛(들), 농축물(들), 프리믹스(들), 부산물(들), 그레인(들), 디스틸러(distiller) 그레인(들), 당밀, 섬유소(들), 꼴(fodder)(들), 그라스(들), 건초, 커넬(들), 잎, 가루(meal), 가용성 물질(들), 및 보충물(들)을 포함한다.“Feed” as used herein refers to material(s) consumed by an animal and contributing energy and/or nutrients to the animal's diet. Examples of feeds include, but are not limited to, fully formulated feed (TMR), feed(s), pellet(s), concentrate(s), premix(s), by-product(s), grain(s), distiller(s) distiller) grain(s), molasses, cellulose(s), fodder(s), grass(s), hay, kernel(s), leaves, meal, soluble material(s), and supplements. Includes (s).
본 발명의 “원료사료(feed ingredient)”란, 동물 또는 가축의 영양소 공급을 위하여 혼입되는 각각의 단일 사료를 뜻한다.“Feed ingredient” of the present invention refers to each single feed mixed to supply nutrients to animals or livestock.
이에, 본 발명의 원료사료의 총에너지, 가소화에너지 또는 대사에너지 함량 분석 방법은, 동애등에 원료사료의 총에너지, 가소화에너지 또는 대사에너지 함량을 분석함으로서, 양돈 사료 성분의 사전 결정된 영양 프로파일을 결정하고, 이에 부합하도록 사료를 제조할 수 있도록 하는 것이었다.Accordingly, the method for analyzing the total energy, digestible energy or metabolizable energy content of the raw feed of the present invention is to analyze the total energy, digestible energy or metabolizable energy content of the raw feed, such as Dongae, to determine the predetermined nutritional profile of the pig feed ingredients. The goal was to make a decision and manufacture feed to comply with that decision.
본 발명의 일실시예에 따르면, 상기 측정된 조단백질, 조지방, 또는 조회분 함량은 하기의 수학식 1 내지 수학식 7로 이루어진 군에서 선택된 방정식에 대입하여, 총에너지, 가소화에너지 또는 대사에너지 함량을 추정하는 것일 수 있다.According to one embodiment of the present invention, the measured crude protein, crude fat, or ash content is substituted into an equation selected from the group consisting of Equations 1 to 7 below to obtain total energy, digestible energy, or metabolizable energy content. It may be an estimate.
본 발명의 일실시예에 따르면, 상기 원료사료는 양돈(pig farming)사료인 것일 수 있다.According to one embodiment of the present invention, the raw feed may be pig farming feed.
본 발명의 일실시예에 따르면, 상기 동애등에는, 동애등에 유충(black soldier fly larvae)인 것일 수 있고, 바람직하게는 건조된 유충인 것일 수 있다.According to one embodiment of the present invention, the black soldier fly larvae may be black soldier fly larvae, and preferably, they may be dried larvae.
또한, 본 발명은 동애등에(Hermetia illucens)의 조단백질, 조지방 또는 조회분 함량(%) 측정부; 및In addition, the present invention provides a measuring unit for crude protein, crude fat or ash content (%) of black soldier fly ( Hermetia illucens ); and
상기 측정된 조단백질, 조지방 또는 조회분 함량으로부터, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량을 연산하는 연산부를 포함하는, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량 측정 장치를 제공한다.Total energy of feed ingredients for black soldier flies, etc., including a calculation unit that calculates the total energy, digestible energy, or metabolizable energy content of the feed ingredients for black soldier flies, etc., from the measured crude protein, crude fat, or ash content. , Provides a device for measuring digestible energy or metabolizable energy content.
본 발명의 일실시예에 따르면, 상기 측정된 조단백질, 조지방 또는 조회분 함량은, 하기 수학식 1 내지 수학식 7의 방정식에 대입되어, 연산되는 것일 수 있다.According to one embodiment of the present invention, the measured crude protein, crude fat or ash content may be calculated by substituting into the equations of Equations 1 to 7 below.
이하, 본 발명을 실시예에 의하여 더욱 상세하게 설명한다. 이들 실시예는 단지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예에 국한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail through examples. These examples are merely for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited to these examples.
<실시예 1> 동애등애 원료사료 화학 분석<Example 1> Chemical analysis of black soldier fly raw material feed
본 발명의 동애등에(Hermetia illucens, black soldier fly larvae) 원료사료 내 영양소 분석을 위하여, 화학 분석을 수행하였다. 구체적으로 건조된 동애등에를 직경 1 mm 이하로 분쇄하고, 건물(AOAC, 2019; method 930.15), 조단백질(AOAC, 2019; method 990.03), 조지방(AOAC, 2019; method 920.39), 조회분(AOAC, 2019; method 942.05) 및 산성세제불용성섬유소(AOAC, 2019; method 973.18)를 파이버텍(FibertecTM) 시스템을 사용하여 분석하였다. 또한, 동애등에 내 총에너지 함량은 봄베 열량계(Parr 6200, Parr Instruments Co., Moline, IL, USA)를 이용하였다. 동애등에 원료사료 화학 분석의 결과는 하기 표 1에 나타내었다.To analyze nutrients in the raw soldier feed of the present invention ( Hermetia illucens , black soldier fly larvae), chemical analysis was performed. Specifically, dried black soldier flies were pulverized to a diameter of 1 mm or less, and dried (AOAC, 2019; method 930.15), crude protein (AOAC, 2019; method 990.03), crude fat (AOAC, 2019; method 920.39), and ash (AOAC, 2019; method 942.05) and acid detergent-insoluble fiber (AOAC, 2019; method 973.18) were analyzed using the FibertecTM system. In addition, the total energy content in black soldier flies was measured using a bomb calorimeter (Parr 6200, Parr Instruments Co., Moline, IL, USA). The results of the chemical analysis of the raw material feed for Dongae, etc. are shown in Table 1 below.
그 결과, 상기 표 1에서와 같이, 동애등에 원료사료 내 총 에너지(kcal)는 1 kg당 4402 내지 5875 kcal이며, 조단백질은 36.5 내지 62.0%, 조지방은 9.0 내지 38.5%, 조회분은 13.7 내지 25.3% 및 산성세제불용성섬유소는 7.4 내지 13.6%인 것을 확인하였다.As a result, as shown in Table 1, the total energy (kcal) in the raw material feed for black soldier flies is 4402 to 5875 kcal per kg, crude protein is 36.5 to 62.0%, crude fat is 9.0 to 38.5%, and ash is 13.7 to 25.3%. % and acid detergent insoluble fiber were confirmed to be 7.4 to 13.6%.
<실시예 2> 동애등에 원료사료의 가소화에너지 및 대사에너지 함량 측정<Example 2> Measurement of digestible energy and metabolizable energy content of raw material feed for Dongae, etc.
본 발명의 동애등에 원료사료의 가소화에너지 및 대사에너지 함량을 측정하였다. 구체적으로, 6마리의 거세 수퇘지(개시체중 = 77.0 ± 3.7 kg)를 사용하여, 가소화에너지 및 대사에너지 함량을 측정하였으며, 옥수수를 원료사료로 이용한 기초사료 1종과, 기초사료의 25%씩을 동애등에로 대체한 5종의 실험사료를 준비하였다. 비타민과 미네랄은 NRC(2012)의 요구량을 충족시키거나 초과하도록 하였다. 6마리의 거세 수퇘지와 7개의 period를 갖는 6 × 7 라틴방격법으로 돼지에게 6개의 실험사료를 급여하였다. 그 후 Potential carry over effects를 보정하기 위하여, spreadsheet program을 사용하였으며, 실험사료의 급여랑은 대사체 체중의 9%에 해당하는 양을 동일하게 반으로 나누어 오전 8시와 오후 5시에 급여하였다. 각 period의 초기 4일은 실험사료에 대한 적응기였으며, 적응기간 이후 4일간 분 및 뇨를 채취하였다. 분 채취는 marker-tomarker method를 이용하였으며, 뇨는 분 채취 기간동안 전량 채취하였다. 전량 채취한 분 및 뇨는 -20℃에서 냉동보관 하였다. 분 및 뇨 내의 총에너지 함량을 봄베 열량계로 측정하였으며, 이를 이용하여 동애등에 원료사료 내 가소화에너지 및 대사에너지 함량을 결정하였다.The digestible energy and metabolizable energy content of the raw feed for black soldier flies of the present invention were measured. Specifically, six castrated boars (starting weight = 77.0 ± 3.7 kg) were used to measure digestible energy and metabolizable energy content, and one type of basic feed using corn as the raw feed and 25% of the basic feed were used. Five types of experimental feed replaced with black soldier fly were prepared. Vitamins and minerals met or exceeded NRC (2012) requirements. Six experimental feeds were fed to pigs using a 6 × 7 Latin square system with 6 castrated boars and 7 periods. Afterwards, to correct for potential carry over effects, a spreadsheet program was used, and the amount equivalent to 9% of the metabolite body weight of the experimental feed was divided equally and fed at 8 a.m. and 5 p.m. The first 4 days of each period were an adaptation period to the experimental feed, and feces and urine were collected for 4 days after the adaptation period. The marker-tomarker method was used for fecal collection, and the entire amount of urine was collected during the fecal collection period. All collected feces and urine were stored frozen at -20°C. The total energy content in feces and urine was measured using a bomb calorimeter, and this was used to determine the digestible energy and metabolizable energy content in the raw material feed for black soldier flies.
<실시예 3> 동애등에 원료사료 가소화에너지 및 대사에너지 추정식 도출<Example 3> Derivation of equations for estimating digestibility and metabolic energy of raw material feed for Dongae, etc.
상기 실시예 1의 동애등에의 영양소 함량 및 실시예 2에서 도출한 돼지 분 및 뇨 내 가소화에너지 및 대사에너지 함량을 이용하여, 동애등에 원료사료 내 영양소 함량에 따른 가소화에너지 및 대사에너지 추정식을 도출하였다. 구체적으로 실험사료 내 조단백질, 조지방, 조회분, 산성세제불용성섬유소 농도와, 동애등에 내 총에너지, 가소화에너지 및 대사에너지 함량 간의 상관관계는 SAS(SAS Inst. Inc., Cary, NC, USA)의 Proc CORR를 이용하여 도출하였다. 또한, 다양한 동애등에 원료사료 내 총에너지, 가소화에너지 및 대사에너지 함량에 대한 추정식은 SAS의 Reg procedure를 이용하여 도출하였다. 모든 분석의 통계적 유의성은 P < 0.05일 때 통계적으로 유의하다고 판정하였다. 그 결과를 하기 표 2에 나타내었다.Using the nutrient content of black soldier fly in Example 1 and the digestible energy and metabolic energy content in pig feces and urine derived in Example 2, the digestible energy and metabolic energy estimation formula according to the nutrient content in the raw soldier feed for black soldier fly, etc. was derived. Specifically, the correlation between the concentrations of crude protein, crude fat, ash, and acid detergent-insoluble fiber in the experimental feed and the total energy, digestible energy, and metabolizable energy content in black soldier flies were analyzed by SAS (SAS Inst. Inc., Cary, NC, USA). It was derived using Proc CORR. In addition, the estimation formulas for the total energy, digestible energy, and metabolizable energy content in raw material feed for various black soldier flies were derived using the Reg procedure of SAS. The statistical significance of all analyzes was determined to be statistically significant when P < 0.05. The results are shown in Table 2 below.
상기 표 2의 결과로 동애등에 원료사료(건물기준)내 총에너지, 가소화에너지 및 대사에너지를 추정할 수 있는 하기의 수학식 1 내지 수학식 7을 도출하였다.As a result of Table 2 above, the following equations 1 to 7 were derived, which can estimate the total energy, digestible energy, and metabolic energy in the raw feed (building basis) for black soldier flies.
[수학식 1][Equation 1]
[수학식 2][Equation 2]
[수학식 3][Equation 3]
[수학식 4][Equation 4]
[수학식 5][Equation 5]
[수학식 6][Equation 6]
[수학식 7][Equation 7]
또한, 상기 수학식 1 내지 수학식 7의 통계적 유의성을 확인한 결과, 수학식 1은 R 2 = 0.98, P = 0.018, 수학식 2는 R 2 = 0.99, P = 0.005, 수학식 3은 R 2 = 0.98, P = 0.023, 수학식 4는 R 2 = 0.99, P = 0.009, 수학식 5는 R 2 = 0.92, P = 0.009, 수학식 6은 R 2 = 0.97, P = 0.028, 수학식 7은 R 2 = 0.99, P = 0.009인 것을 확인하여, 본 발명의 수학식 1 내지 수학식 7의 방정식은 P < 0.05인 것을 확인하여, 통계적으로 유의한 추정 방정식인 것을 확인하였다.In addition, as a result of confirming the statistical significance of Equations 1 to 7, Equation 1 was R 2 = 0.98, P = 0.018, Equation 2 was R 2 = 0.99, P = 0.005, and Equation 3 was R 2 = 0.005. 0.98, P = 0.023, Equation 4 is R 2 = 0.99, P = 0.009, Equation 5 is R 2 = 0.92, P = 0.009, Equation 6 is R 2 = 0.97, P = 0.028, Equation 7 is R 2 It was confirmed that 2 = 0.99, P = 0.009, and the equations of Equations 1 to 7 of the present invention were confirmed to have P < 0.05, confirming that they were statistically significant estimation equations.
따라서, 본 발명은 동애등에의 조단백질, 조지방, 조회분 및 산성세제불용성섬유소 함량을 확인하여, 동애등에 원료사료의 총에너지 함량을 추정할 수 있는 방법을 도출하였다. 또한, 동애등에를 원료사료를 동물모델에 급여한 후, 동애등에 원료사료의 가소화에너지 및 대사에너지 함량을 확인하였으며, 이를 통해 통계적으로 유의한 동애등에 원료사료의 가소화에너지 및 대사에너지 추정 방법을 도출하였다.Therefore, the present invention identified the crude protein, crude fat, ash, and acid detergent-insoluble fiber contents of black soldier flies, and derived a method for estimating the total energy content of the raw feed for black soldier flies. In addition, after feeding black soldier fly raw feed to an animal model, the digestible energy and metabolic energy content of the raw black soldier feed were confirmed, and through this, a statistically significant method of estimating the digestible energy and metabolic energy of the raw black soldier feed was found. was derived.
Claims (8)
상기 측정된 조단백질, 조지방 또는 조회분 함량으로부터, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량 측정 방법.Measuring the crude protein, crude fat or ash content (%) of black soldier fly ( Hermetia illucens ); and
A method of measuring the total energy, digestible energy, or metabolizable energy content of feed ingredients for black soldier flies, etc., from the measured crude protein, crude fat, or ash content.
상기 측정된 조단백질, 조지방, 또는 조회분 함량은 하기의 수학식 1 내지 수학식 7로 이루어진 군에서 선택된 방정식에 대입하여, 총에너지, 가소화에너지 또는 대사에너지 함량을 추정하는 것인, 방법
[수학식 1]
;
[수학식 2]
;
[수학식 3]
;
[수학식 4]
;
[수학식 5]
;
[수학식 6]
; 및
[수학식 7]
.According to clause 1,
The measured crude protein, crude fat, or ash content is substituted into an equation selected from the group consisting of Equations 1 to 7 below to estimate total energy, digestible energy, or metabolizable energy content.
[Equation 1]
;
[Equation 2]
;
[Equation 3]
;
[Equation 4]
;
[Equation 5]
;
[Equation 6]
; and
[Equation 7]
.
상기 원료사료는 양돈(pig farming)사료인 것인, 방법.According to clause 1,
The method wherein the raw feed is pig farming feed.
상기 동애등에는, 동애등에 유충(black soldier fly larvae)인 것인, 방법.According to clause 1,
The method wherein the black soldier fly larvae are black soldier fly larvae.
상기 동애등에 유충은, 건조된 것인, 방법.According to clause 4,
The method wherein the larvae of the black soldier fly are dried.
상기 측정된 조단백질, 조지방 또는 조회분 함량으로부터, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량을 연산하는 연산부를 포함하는, 동애등에 원료사료(feed ingredients)의 총에너지, 가소화에너지 또는 대사에너지 함량 측정 장치.Measuring unit for crude protein, crude fat or ash content (%) of black soldier fly ( Hermetia illucens ); and
Total energy of feed ingredients for black soldier flies, etc., including a calculation unit for calculating the total energy, digestible energy, or metabolizable energy content of the feed ingredients for black soldier flies, etc., from the measured crude protein, crude fat, or ash content. , Device for measuring digestible energy or metabolizable energy content.
상기 측정된 조단백질, 조지방 또는 조회분 함량은, 하기 수학식 1 내지 수학식 7의 방정식에 대입되어, 연산되는 것인, 장치
[수학식 1]
;
[수학식 2]
;
[수학식 3]
;
[수학식 4]
;
[수학식 5]
;
[수학식 6]
; 및
[수학식 7]
.According to clause 6,
The measured crude protein, crude fat or ash content is calculated by substituting into the equations of Equation 1 to Equation 7 below:
[Equation 1]
;
[Equation 2]
;
[Equation 3]
;
[Equation 4]
;
[Equation 5]
;
[Equation 6]
; and
[Equation 7]
.
상기 원료사료는 양돈(pig farming)사료인 것인, 장치.According to clause 6,
The device wherein the raw feed is pig farming feed.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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