KR20240021893A - 데아미나제 기반 rna 센서 - Google Patents

데아미나제 기반 rna 센서 Download PDF

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Publication number
KR20240021893A
KR20240021893A KR1020247001214A KR20247001214A KR20240021893A KR 20240021893 A KR20240021893 A KR 20240021893A KR 1020247001214 A KR1020247001214 A KR 1020247001214A KR 20247001214 A KR20247001214 A KR 20247001214A KR 20240021893 A KR20240021893 A KR 20240021893A
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South Korea
Prior art keywords
rna
sensor
adar
sensor system
ssrna
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Pending
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Korean (ko)
Inventor
카이이 장
로한 네일 크라제스키
오마르 오사마 아부데이예
조나단 에스. 구텐베르크
이판 장
페이 첸
시 첸
제레미 지. 쿱
Original Assignee
메사추세츠 인스티튜트 오브 테크놀로지
더 브로드 인스티튜트, 인코퍼레이티드
프레지던트 앤드 펠로우즈 오브 하바드 칼리지
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Publication of KR20240021893A publication Critical patent/KR20240021893A/ko
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04004—Adenosine deaminase (3.5.4.4)
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/327—RNAse, e.g. RNAseH
    • C—CHEMISTRY; METALLURGY
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/20—Detection means characterised by being a gene reporter based analysis
    • C—CHEMISTRY; METALLURGY
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06N—COMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
    • G06N3/00—Computing arrangements based on biological models
    • G06N3/12—Computing arrangements based on biological models using genetic models
    • G06N3/123—DNA computing

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
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  • Biomedical Technology (AREA)
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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Bioinformatics & Computational Biology (AREA)
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  • General Physics & Mathematics (AREA)
  • Mathematical Physics (AREA)
  • Software Systems (AREA)
  • Artificial Intelligence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020247001214A 2021-06-15 2022-06-14 데아미나제 기반 rna 센서 Pending KR20240021893A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202163210829P 2021-06-15 2021-06-15
US63/210,829 2021-06-15
US202263267177P 2022-01-26 2022-01-26
US63/267,177 2022-01-26
PCT/US2022/033459 WO2022266117A2 (en) 2021-06-15 2022-06-14 Deaminase-based rna sensors

Publications (1)

Publication Number Publication Date
KR20240021893A true KR20240021893A (ko) 2024-02-19

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KR1020247001214A Pending KR20240021893A (ko) 2021-06-15 2022-06-14 데아미나제 기반 rna 센서

Country Status (9)

Country Link
US (2) US12195734B2 (https=)
EP (1) EP4355906A2 (https=)
JP (1) JP7795747B2 (https=)
KR (1) KR20240021893A (https=)
AU (1) AU2022291759A1 (https=)
CA (1) CA3222560A1 (https=)
IL (1) IL309025B1 (https=)
MX (1) MX2023014550A (https=)
WO (1) WO2022266117A2 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2024004934A (es) * 2021-10-29 2024-09-10 Duke Univeristy Composiciones y sistemas para la edicion de celulas programables por arn y metodos para elaborar y usar los mismos.
KR20240155250A (ko) * 2022-02-24 2024-10-28 더 보드 오브 트러스티스 오브 더 리랜드 스탠포드 쥬니어 유니버시티 감각 반응 응용을 위해 adar 편집을 이용하는 살아있는 세포의 rna 센서
JPWO2024071424A1 (https=) 2022-09-29 2024-04-04
JP7847274B2 (ja) * 2022-10-27 2026-04-16 アリゾナ ボード オブ リージェンツ オン ビハーフ オブ アリゾナ ステート ユニバーシティ 核酸センサーに関連する組成物および方法
WO2025054355A1 (en) * 2023-09-06 2025-03-13 Radar Therapeutics Inc. Methods, systems, and compositions for exogenous control of protein expression
WO2025151800A1 (en) 2024-01-10 2025-07-17 Autonomous Therapeutics, Inc. Programmable, rna editor-controlled nucleic acid dose amplifiers and their methods of use
CN119505015B (zh) * 2024-11-15 2025-10-17 北京大学 一种融合蛋白及其应用方法
CN121344095B (zh) * 2025-12-16 2026-04-28 北京肿瘤医院(北京大学肿瘤医院) 一种细胞内mRNA传感器开关的DNA文库筛选方法及应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015120197A1 (en) * 2014-02-05 2015-08-13 Regents Of The University Of California Rna editing biomarkers for diagnosis, pharmacological screening and prognostication in cancer
CN110612353A (zh) * 2017-03-03 2019-12-24 加利福尼亚大学董事会 经由抑制性tRNAs和脱氨酶对突变进行RNA靶向
US11359247B2 (en) * 2017-07-31 2022-06-14 Massachusetts Institute Of Technology High performance multi-input microRNA sensors and uses thereof
WO2019071048A1 (en) * 2017-10-04 2019-04-11 The Broad Institute, Inc. SYSTEMS, METHODS AND COMPOSITIONS FOR TARGETED NUCLEIC ACID EDITION
EP3864152B1 (en) * 2018-10-12 2023-08-16 Peking University Methods and compositions for editing rnas
WO2022003684A1 (en) * 2020-06-29 2022-01-06 Bar-Ilan University Composition and methods for identifying antisense guide rna for rna editing
MX2024004934A (es) * 2021-10-29 2024-09-10 Duke Univeristy Composiciones y sistemas para la edicion de celulas programables por arn y metodos para elaborar y usar los mismos.
KR20240155250A (ko) * 2022-02-24 2024-10-28 더 보드 오브 트러스티스 오브 더 리랜드 스탠포드 쥬니어 유니버시티 감각 반응 응용을 위해 adar 편집을 이용하는 살아있는 세포의 rna 센서

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Publication number Publication date
IL309025B1 (en) 2026-03-01
US20250101426A1 (en) 2025-03-27
IL309025A (en) 2024-02-01
JP7795747B2 (ja) 2026-01-08
US20230123513A1 (en) 2023-04-20
CA3222560A1 (en) 2022-12-22
MX2023014550A (es) 2024-02-28
AU2022291759A1 (en) 2023-12-21
WO2022266117A3 (en) 2023-03-02
US12195734B2 (en) 2025-01-14
WO2022266117A2 (en) 2022-12-22
JP2024523877A (ja) 2024-07-02
EP4355906A2 (en) 2024-04-24

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