KR20230161837A - Electroporation dispenser with disposable cartridge - Google Patents

Abstract

The present invention is a dispenser for electroporation that includes a disposable cartridge that receives voltage from the main body and injects electroporation and drugs into the electroporation and drug injection target, forming steps and step spaces 10 at the corners, and forming a step space 10 on the inside. A case part 100 provided with a first receiving space; and a disposable case part 100 that is detachable from the step space of the case part 100 and can be combined with the case part 100, and has a second receiving space therein. Cartridge 200; and a power generating means 300 provided inside the case portion 100 to provide power to the disposable cartridge 200; and provided inside the case portion 100 to generate voltage for the main body. A voltage unit 400 that receives voltage from the means and transmits the applied voltage to the electroporer; and a control unit 500 that integratedly controls the power generation means 300 and the voltage unit 400. It relates to a dispenser for electroporation comprising a disposable cartridge.

Description

Dispenser for electroporation comprising disposable cartridges {ELECTROPORATION DISPENSER WITH DISPOSABLE CARTRIDGE}

The present invention relates to a dispenser for electroporation including a disposable cartridge, and more specifically, to minimize the distance at which the drug is injected by receiving voltage from the main body and injecting electroporation and drug into the target for electroporation and drug injection, As a result, it is possible to minimize the amount of drug used, and furthermore, to simply dispose of highly toxic remaining drugs by replacing disposable cartridges, and to use a dispenser for electroporation that includes a disposable cartridge that ensures safety for delivering high voltage. It's about.

A tumor refers to cells in the body that have grown autonomously and excessively. Tumors develop regardless of the overall health of the organism. Tumors are divided into malignant tumors and benign tumors, and malignant tumors are called cancer. Since benign tumors are not cancerous, there is no major problem if they are resected according to the area.

To treat a tumor, surgical methods to excise the tumor, a method to destroy the tissue by irradiating the tumor, and a method to kill cancer cells, which are malignant tumors, by administering anticancer drugs are used. For early-stage cancerous tumors, surgical methods to excise the tumor can be used. However, there is a risk for malignant tumors, and although radiation therapy or chemotherapy treatment is initially effective, it is not effective for malignant tumors.

Moreover, since the patient's immune system is low and the body becomes weak, it cannot be applied to weak patients. Therefore, a method of administering anticancer drugs to kill cancer cells, which are malignant tumors, must be used. A method of administering anticancer drugs to malignant tumors is necessary. Electroporation is used.

Electroporation is a technology that forms nanometer-sized pores on the surface of cell membranes by applying electric pulses with a width of nanoseconds to milliseconds to cells through electrical stimulation at a certain range of intensity. Since its discovery in the 1970s, it has been used in various biotechnologies for decades. applied to the field. Micropores on the cell membrane surface created under appropriate electric pulse conditions have the reversible property of disappearing again after a certain period of time, which is called reversible electroporation. It is mainly used to inject substances such as hydrophilic drugs, genes, enzymes, and antibodies into cells. It was used to

On the other hand, fine pores created by stronger electric pulses do not disappear and act as a mechanism that ultimately causes the cells to lose their vitality, which is called irreversible electroporation. Irreversible electroporation technology is not a desirable phenomenon in terms of reversible electroporation, but on the contrary, technological approaches that take advantage of its advantages are also being made.

Conventional electroporators capable of such electroporation usually include a main body that outputs electrical signals or pulse-type electrical signals; a monitoring unit that monitors electrical signals output from the main body; An alarm unit that generates an alarm signal when a failure is detected in the monitoring unit and generates a treatment completion signal when treatment is completed; a storage unit that stores patient data or treatment data; A display unit that displays a patient data input screen or treatment screen; An input unit that receives patient data or treatment setting data; and a control unit that sets the electric signal according to the treatment setting data input from the input unit and controls the main body unit so that the electric signal is applied, and the output pulse is applied using a separate handpiece-type electrode applicator or a drug applicator. A common configuration is to supply it to the target area through a fusion-type applicator integrated into the injection needle.

However, when supplying drugs from the main body to the handpiece-type electrode applicator, the drug injection line is long, so expensive drugs remain inside the drug injection line, and a large amount of expensive drugs are discarded, and drugs such as anticancer drugs Because silver is very toxic, the processing process was very complicated and cumbersome.

In relation to this, according to the patent document Republic of Korea Patent Publication No. 10-0756252 (registered on August 31, 2007, title of invention: method and apparatus for using electroporation mediated delivery of drugs and genes) , " relates to a method and device for electroporation therapy in vivo, using electroporation therapy (EPT) to treat tumors by combining electroporation using the device of the present invention, and the chemical therapeutic agent is used to treat tumors in vivo. An EPT method that uses long pulses of low voltage to kill cells for the purpose of suppressing the tumor. In one embodiment, an array of needles with key elements that determine the set point of the therapeutic voltage pulses and/or the selective array switching pattern. A technology called “A system for clinical electroporation equipped with electrodes” is disclosed.

However, the above patent document discloses a pulse generator that includes an electroporation treatment device and an electrode applicator and can be programmed and controlled for pulse duration, voltage level, output, etc. for the electrode applicator, and the generated pulse is generated by the electrode applicator. It is delivered into the tissue through at least one electrode needle of the applicator. In addition, the applicator is relevant in that it can connect a drug supply unit to a tube as well as an electrode, enabling appropriate pulse supply and drug injection to the tissue area, but only the basic configuration of the reversible electroporation technology is disclosed. In addition, there was no disclosure at all about the configuration of the distributor connected between the pulse generator and the electrode applicator, the configuration for efficiently processing the drug, and the characteristic configuration of the technology that can minimize the amount of drug used.

Therefore, by receiving voltage from the main body and electroporating and injecting the drug into the target, the distance at which the drug is injected is minimized, ultimately minimizing the amount of drug used, and further, removing the highly toxic residual drug into the disposable cartridge. There is a need for a dispenser for electroporation that contains disposable cartridges that can be simply discarded for replacement.

1. Republic of Korea Patent No. 10-0756252 (registered on August 31, 2007, title of invention: method and apparatus for using electroporation mediated delivery of drugs and genes)

The technical problem to be achieved by the present invention is to solve the problems or needs described above, and is an electroporation device comprising a disposable cartridge for electroporation and drug injection into a subject for electroporation and drug injection by receiving voltage from the main body. It provides a distributor.

Another object of the present invention is to minimize the distance at which the drug is injected, thereby reducing the amount of drug remaining inside the drug injection line, thereby minimizing the amount of drug used, and further replacing the highly toxic remaining drug with a disposable cartridge. The aim is to provide a dispenser for electroporation containing a disposable cartridge that can be simply disposed of.

Meanwhile, the technical problems to be achieved in the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly apparent to those skilled in the art from the description below. It will be understandable.

The electroporation dispenser including a disposable cartridge according to an embodiment of the present invention for realizing the above-described problem is a disposable cartridge that receives voltage from the main body and injects electroporation and drug into the electroporation and drug injection target. A distributor for electroporation, including a case portion 100 that forms steps and step spaces at the corners and has a first receiving space therein.

Here, a disposable cartridge 200 that is detachable from the step space of the case part 100 and can be combined with the case part 100 and has a second receiving space therein; and the case part 100 ) Power generation means 300 provided inside to provide power to the disposable cartridge 200.

Here, a voltage unit 400 provided inside the case unit 100 to receive voltage from the voltage generation means of the main body and transmit the applied voltage to the electroporator; and the power generation means 300 and the voltage It includes a control unit 500 that comprehensively controls the unit 400.

At this time, the control unit 500 is provided with a first connection port 510 so that the case unit 100 and the disposable cartridge 200 are coupled to the step surface 110 formed at the step of the case unit 100. When the case portion 100 and the disposable cartridge 200 are coupled through the first connection port 510, the power generated by the power generation means 300 is transferred to the disposable cartridge 200. It is possible to convey it.

In addition, the disposable cartridge 200 includes a drug injection port 210 for injecting a drug into the disposable cartridge 200; A second connection port (220) for injecting a drug into a drug injector; And a drug transfer unit 230, one end of which is connected to the drug injection port 210 and the other end of which is connected to the second connection port 220, so as to inject the drug injected from the drug injection port 210 into the drug injector. provided, and the second connection port 220 may be provided with one or more so that one or more drug injectors can be connected.

Meanwhile, the drug transport unit 230 is provided in a branched structure to supply drug to each drug injector connected to the second connection port 220 when the second connection port 220 is provided in plurality. It can be.

At this time, the drug transport unit 230 is further provided with a peristaltic tubing pump to improve the flow rate precision of the injected drug when injecting the drug into the drug injector, and the peristaltic tubing pump generates the power. It operates by receiving power from the means 300, and the drug injector may be integrally coupled to the inside of the electroporator.

Here, the voltage unit 400 is provided with a plurality of connection terminals 410 that are connected to the voltage generating means and receive voltage from the voltage generating means, and the control unit 500 is connected to the voltage generating means. A voltage providing means 520 for transmitting the applied voltage to the electroporator is further provided, and the voltage providing means 520 may be provided in a number corresponding to the number of the second connection ports 220.

In addition, a coupling guider 111 is provided at both ends of the stepped surface 110 and is provided with a coupling groove 111a so that the disposable cartridge 200 can be easily coupled to the case part 100. Both sides 240 of the disposable cartridge 200 are further provided with engaging protrusions 241 that correspond to the shape of the engaging groove 111a and are coupled thereto.

Here, the control unit 500 may be further provided with an emergency stop means 530 that can immediately stop the operation of the power generation means 300 and the voltage unit 400 to respond to emergency situations that may occur during the procedure. You can.

Meanwhile, the upper surface 120 of the case portion 100 is provided with a rotating fixture 130 to improve the fixing force of the disposable cartridge 200 when the disposable cartridge 200 and the case portion 100 are combined. It is further provided, and the rotating fixture 130 is provided with a rotating shaft 131 and a rotating means 132 that rotates around the rotating shaft 131 so that the disposable cartridge 200 and the case portion 100 Before coupling, the rotation means 132 is fixed at the position of the upper surface 120, and when the disposable cartridge 200 and the case portion 100 are combined, the rotation means 132 is the stepped surface ( The disposable cartridge 200 can be easily fixed by rotating in the direction 110).

Accordingly, according to the electroporation dispenser including a disposable cartridge according to the present invention,

First, by receiving voltage from the main body and electroporating and injecting drugs into the target, the drug injection line is minimized and the voltage is supplied safely, ultimately preventing drug abuse and ensuring safety. There is an effect.

Second, the use of a peristaltic tubing pump when injecting a drug improves flow rate precision, and furthermore allows the drug to be easily injected into multiple electroporators.

Third, since the disposable cartridge is equipped with a drug injection port and a drug transfer portion, the remaining drug can be easily disposed of by simply replacing the disposable cartridge, providing high safety and convenience to the user.

Fourth, a coupling guider and a coupling protrusion are provided so that the case portion and the disposable cartridge can be easily coupled, and furthermore, the rotating fixture can prevent the disposable cartridge from bobbing up and down.

Fifth, by unifying the connection wire to the main body, it is possible to simply connect multi-channel fusion electrodes, deriving the need to ensure the safety of high-voltage electroporation voltage transmission, and having the effect of resolving complexity in clinical settings and improving usability. .

Meanwhile, the effects that can be obtained from the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below. You will be able to.

The following drawings attached to this specification illustrate a preferred embodiment of the present invention, and serve to further understand the technical idea of the present invention along with the detailed description of the invention. Therefore, the present invention is limited to the matters described in such drawings. It should not be interpreted in a limited way.
Figure 1 is a diagram for explaining the overall configuration of a dispenser for electroporation including a disposable cartridge according to a preferred embodiment of the present invention.
Figure 2 is a view for explaining a state in which a case portion of an electroporation dispenser including a disposable cartridge and a disposable cartridge are combined according to a preferred embodiment of the present invention.
Figure 3 is a perspective view for explaining the power generating means and the voltage portion of the electroporation dispenser including a disposable cartridge according to a preferred embodiment of the present invention.
Figures 4 and 5 are diagrams for explaining the coupling guider, coupling protrusion, and rotational fixture of the electroporation dispenser including a disposable cartridge according to a preferred embodiment of the present invention.

Specific structural or functional descriptions of the embodiments according to the concept of the present invention disclosed in this specification are merely illustrative for the purpose of explaining the embodiments according to the concept of the present invention, and the embodiments according to the concept of the present invention are It may be implemented in various forms and is not limited to the embodiments described herein.

Since the embodiments according to the concept of the present invention can make various changes and have various forms, the embodiments will be illustrated in the drawings and described in detail in this specification. However, this is not intended to limit the embodiments according to the concept of the present invention to specific disclosed forms, and includes all changes, equivalents, or substitutes included in the spirit and technical scope of the present invention.

Electroporation is a technology that forms nanometer-sized pores on the surface of cell membranes by applying electric pulses with a width of nanoseconds to milliseconds to cells through electrical stimulation at a certain range of intensity. Since its discovery in the 1970s, it has been used in various biotechnologies for decades. applied to the field. Micropores on the cell membrane surface created under appropriate electric pulse conditions have the reversible property of disappearing again after a certain period of time, which is called reversible electroporation. It is mainly used to inject substances such as hydrophilic drugs, genes, enzymes, and antibodies into cells. It was used to

When the electroporator for conventional electroporation supplies drugs from the main body to the handpiece-type electrode applicator, the drug injection line is long, so a large amount of expensive drugs are discarded, and drugs such as anticancer drugs are very toxic. Therefore, the processing process was very complicated and cumbersome.

Accordingly, the present invention minimizes the distance at which the drug is injected by receiving voltage from the main body and electroporating and injecting the drug into the target, thereby minimizing the amount of drug used and further eliminating highly toxic residual drugs. This is a dispenser for electroporation that includes a disposable cartridge that is simply discarded by replacing the disposable cartridge, and will be described below with reference to the drawings.

Figure 1 is a diagram for explaining the overall configuration of an electroporation dispenser including a disposable cartridge according to a preferred embodiment of the present invention, and Figure 2 is a diagram showing an electroporation including a disposable cartridge according to a preferred embodiment of the present invention. This is a drawing to explain how the case part of a common dispenser and a disposable cartridge are combined.

According to an embodiment of the present invention, an electroporation dispenser including a disposable cartridge that receives voltage from the main body and injects electroporation and drug into the electroporation and drug injection target is first, as shown in Figures 1 and 2. A step and a step space 10 are formed at the corners, and the case portion 100 is provided with a first receiving space therein and is detachable from the step space 10 of the case portion 100. It is possible to combine with and includes a disposable cartridge 200 provided with a second receiving space therein.

Figure 3 is a perspective view for explaining the power generation means and the voltage portion of the electroporation dispenser including a disposable cartridge according to a preferred embodiment of the present invention.

Here, as shown in FIG. 3, a power generating means 300 is provided inside the case portion 100 to provide power to the disposable cartridge 200, and a power generating means 300 is provided inside the case portion 100 to provide power to the main body. It includes a voltage unit 400 that receives voltage from the voltage generation means and transmits the applied voltage to the electroporator, and a control unit 500 that integratedly controls the power generation means 300 and the voltage unit 400. do.

At this time, the electroporator usually includes a main body unit that outputs an electrical signal or pulse-type electrical signal, a monitoring unit that monitors the electrical signal output from the main unit, and when a failure is detected in the monitoring unit, an alarm tone is generated, and the treatment ends. an alarm unit that generates a treatment end signal, a storage unit that stores patient data or treatment data, a display unit that displays a patient data input screen or treatment screen, an input unit that receives patient data or treatment setting data, and a storage unit that stores patient data or treatment data. It includes a control unit that sets the electric signal according to the input treatment setting data and controls the main body so that the electric signal is applied, and the output pulse is transmitted using a separate handpiece-type electrode applicator or a needle for injecting the drug. It can be configured to be supplied to the target area through a convergence-type applicator integrated into the.

Here, the control unit 500 is provided with a first connection port 510 to couple the case unit 100 and the disposable cartridge 200 to the step surface 110 formed at the step of the case unit 100. When the case portion 100 and the disposable cartridge 200 are coupled through the first connection port 510, the power generated by the power generation means 300 is transferred to the disposable cartridge 200. It is possible to convey it.

Here, the power generating means 300 is preferably provided at a position directly below the disposable cartridge when the disposable cartridge is coupled with the cage.

In addition, the disposable cartridge 200 includes a drug injection port 210 for injecting a drug into the disposable cartridge 200, a second connection port 220 for injecting a drug into the drug injector, and a drug injection port 210. A drug transfer unit 230 is further provided, one end of which is connected to the drug injection port 210 and the other end of which is connected to the second connection port 220 to inject the drug injected from the drug injector, and the second connection It is preferable that the port 220 is provided with one or more ports 220 so that one or more drug injectors can be connected.

That is, the case portion 100 is capable of receiving voltage from the main body and transmitting the applied voltage to the electroporator, and the disposable cartridge 200 receives the drug and transfers the injected drug to the drug transfer channel, which is the drug transfer channel. It is possible to deliver the drug to the injector through the drug.

In the past, many drugs were abused due to the long distance from the main body to the drug injector. However, through the dispenser of the present invention, electroporation can be performed with minimal drug use by minimizing the drug injection distance, and further, only disposable cartridges with residual drug are disposed of. This can provide users with convenience and safety at the same time.

Here, the drug transport unit 230 has a branched structure to supply drug to each drug injector connected to the second connection port 220 when the second connection port 220 is provided in plurality. It is possible to be provided.

In addition, the drug transport unit 230 is further provided with a peristaltic tubing pump to improve the flow rate precision of the injected drug when injecting the drug into the drug injector, and the peristaltic tubing pump includes the power generation means ( It is desirable to operate by receiving power from 300).

Here, in peristaltic tubing pumps, “Peristaltic” refers to the movement of the “viscera,” which are human organs, and, like the movement of these organs, it operates in a way that the liquid in the tubing is squeezed out by the roller attached to the pump header. It is called a peristaltic tubing pump.

At this time, since non-contact pumping must be performed so that the drug injection flow rate accuracy is within ±15%, a precision motor and peristeltic pumping head are used, and an instantaneous high voltage pulse of 500 to 4,500 V (500 to 1,500 V/cm) is applied to the main body. Since it must continue to operate normally, it is preferable that the drug transport unit be provided with electrical shielding.

In addition, a circuit including an MCU and a high-voltage MOSFET is configured so that two of the six channels can be selectively connected depending on the signal sent from the main body, and a connector for connecting the electroporation instantaneous high-voltage connection line and control communication line connected to the main body and 6 A connector for connecting the dog fusion electrode may be further provided.

Here, the drug injector is integrated and provided inside the electroporator. In other words, when the drug injector is provided inside the electroporator, the electroporator can be connected to the second connection port 220.

Here, the entrance of the drug injection port is preferably provided in a structure corresponding to a drug vial containing an anticancer agent. For example, the inner surface of the entrance of the drug injection port is shaped so that the width through which the drug can be injected gradually narrows as it goes downward, making it possible to form a space into which a drug vial can be easily combined. At this time, since the drug must flow out, it is desirable to narrow it while leaving a certain width.

That is, the drug vial can be easily coupled to the space, so that when delivering the drug to the drug injector, the drug vial can be prevented from being separated from the drug injection port or the drug contained in the drug vial being counted during the procedure.

Meanwhile, the drug injection port may be further provided with a sealing portion so that the entrance is open when administering the anticancer drug and the entrance is closed after administering the anticancer drug. The sealing part is provided as a connecting member, one end of which is coupled near the entrance to the drug injection port, and the other end of which is coupled with a sealing cap that can open or close the entrance of the drug injection port.

For example, when administering an anticancer drug to the drug injection port, open the sealing cap and administer the anticancer drug. At this time, loss of the sealing cap can be prevented through the connecting member. Next, after administering the anticancer drug, the drug injection port can be closed again with a sealing cap to prevent foreign substances from entering the disposable cartridge. In other words, during the electroporation procedure, the inside of the disposable cartridge can be completely sealed.

Here, the disposable cartridge may be provided with different drug injection ports to correspond to the shapes and sizes of different drug vials. For example, it is provided with a disposable cartridge A' having a drug injection port of a shape corresponding to drug A, and a disposable cartridge B' having a drug injection port of a shape corresponding to drug B. Therefore, by providing disposable cartridges with different drug injection ports, it is possible to respond to various procedures and drugs.

Meanwhile, the drug injection port can also be provided to be attached to or detached from a disposable cartridge. If the drug injection port is provided to be removable, only the drug injection port can be replaced depending on the shape and size of the drug vial.

At this time, when the drug injection port is detachable, a step is provided at the bottom of the disposable cartridge to form a step protrusion, and the upper surface of the disposable cartridge may be further provided with a step receiving groove so that the step protrusion is inserted and fixed during stacking. . In other words, as the step protrusion is placed on the step receiving groove, a plurality of disposable cartridges are easily aligned when stacked.

Here, when the drug injection port is provided integrally with the disposable cartridge, an alignment groove is provided on the bottom of the disposable cartridge in a shape corresponding to the shape and size of the drug injection port. That is, the drug injection hole can be used as a member for alignment by being inserted into the alignment groove during stacking.

If the shape of the disposable cartridges is aligned correctly when stacked, the transport of the disposable cartridges is convenient. For example, when disposable cartridges are discarded after use, waste collection companies will be able to safely dispose of large quantities of disposable cartridges at once.

Here, the disposable cartridge is preferably made of an eco-friendly resin material that allows recycling of resources by selectively shredding and recycling when discarded after use, and minimizes the generation of harmful substances when discarded.

Meanwhile, the voltage unit 400 is provided with a plurality of connection terminals 410 that are connected to the voltage generator to receive voltage from the voltage generator, and the control unit 500 receives the voltage applied to the voltage unit 400. It is further provided with a voltage providing means 520 for transmitting a voltage to the electroporator. Preferably, the voltage providing means 520 is provided in a number corresponding to the number of the second connection ports 220.

Figures 4 and 5 are diagrams for explaining the coupling guider, coupling protrusion, and rotational fixture of the electroporation dispenser including a disposable cartridge according to a preferred embodiment of the present invention.

Here, as shown in FIGS. 4 and 5, a coupling guider ( 111) is further provided, and engaging protrusions 241 are further provided on both sides 240 of the disposable cartridge 200 and are coupled to the shape of the engaging groove 111a.

At this time, the control unit 500 is further equipped with an emergency stop means 530 that immediately stops the operation of the power generation means 300 and the voltage unit 400 to respond to emergency situations that may occur during the procedure. .

Here, the upper surface 120 of the case portion 100 is provided with a rotating fixture 130 to improve the holding force of the disposable cartridge 200 when the disposable cartridge 200 and the case portion 100 are combined. It is further provided, and the rotating fixture 130 is provided with a rotating shaft 131 and a rotating means 132 that rotates around the rotating shaft 131 so that the disposable cartridge 200 and the case portion 100 Before coupling, the rotating means 132 is fixed at the position of the upper surface 120, and when the disposable cartridge 200 and the case portion 100 are combined, the rotating means 132 is positioned on the stepped surface 110. By rotating in this direction, the disposable cartridge 200 can be easily fixed.

When using an electroporation dispenser including a disposable cartridge according to an embodiment of the present invention described above, the drug injection line is minimized by receiving voltage from the main body and injecting electroporation and drug into the electroporation and drug injection target. By supplying voltage safely, it is possible to prevent abuse of drugs and ensure safety at the same time. When injecting drugs, the flow precision is improved by using a peristaltic tubing pump, and furthermore, multiple connected electric Drugs can be easily injected into each perforator, and the disposable cartridge is equipped with a drug injection port and a drug transfer section, so the remaining drug can be easily disposed of by simply replacing the disposable cartridge, providing high safety and convenience to the user. A coupling guider and a coupling protrusion are provided so that the disposable cartridge can be easily coupled, and furthermore, the rotating fixture prevents the disposable cartridge from bouncing up and down, and the connection wire to the main body is unified, making multi-channel installation simple. Fusion electrodes can be connected, the need to ensure the safety of high-voltage electroporation voltage delivery is derived, and it has the effect of resolving complexity and improving usability in clinical settings.

The present invention has been described above through a preferred embodiment, but the above-described embodiment is merely an illustrative explanation of the technical idea of the present invention, and various changes are possible within the scope of the technical idea of the present invention. Anyone with ordinary knowledge will be able to understand. Therefore, the scope of protection of the present invention should be interpreted in terms of the matters stated in the patent claims, not the specific embodiments, and all technical ideas within the scope corresponding thereto should be interpreted as being included in the scope of rights of the present invention.

100...case part
110...step surface
111...combined guider
111a...joint groove
120...top surface
130...rotating fixture
131...rotation axis
132...means of meeting
200...disposable cartridge
210...Drug injection port
220...2nd connection port
230...Drug transfer unit
240...both sides
241...combined extrusion
300...means of power generation
400...voltage part
410...connection terminal
500...control unit
510...1st connection port
520...Voltage provision means
530...Emergency stop means

Claims (10)

In the electroporation dispenser including a disposable cartridge that receives voltage from the main body and injects electroporation and drug into the electroporation and drug injection target,
A case portion (100) forming a step and a step space (10) at the corners and having a first receiving space therein;
A disposable cartridge 200 that is detachable from the stepped space of the case 100 and can be coupled to the case 100, and has a second receiving space therein;
Power generation means 300 provided inside the case portion 100 to provide power to the disposable cartridge 200;
A voltage unit 400 provided inside the case unit 100 to receive voltage from the voltage generating means of the main body and transmit the applied voltage to the electroporator; and
A control unit 500 that integratedly controls the power generation means 300 and the voltage unit 400,
Dispenser for electroporation containing disposable cartridges.
According to claim 1,
The control unit 500,
A first connection port 510 is provided on the step surface 110 formed at the step of the case portion 100 to couple the case portion 100 and the disposable cartridge 200,
When the case portion 100 and the disposable cartridge 200 are coupled through the first connection port 510, the power generated by the power generating means 300 is transmitted to the disposable cartridge 200. It is possible,
Dispenser for electroporation containing disposable cartridges.
According to claim 2,
In the disposable cartridge 200,
a drug injection port 210 for injecting drug into the disposable cartridge 200; A second connection port (220) for injecting a drug into a drug injector; And a drug transfer unit 230, one end of which is connected to the drug injection port 210 and the other end of which is connected to the second connection port 220, so as to inject the drug injected from the drug injection port 210 into the drug injector. Equipped with
The second connection port 220 is,
Characterized in that one or more drug injectors are provided so that they can be connected,
Dispenser for electroporation containing disposable cartridges.
According to claim 3,
The drug transport unit 230,
When the second connection port 220 is provided in plurality, it is characterized in that it is provided in a branched structure so that the drug can be supplied to each drug injector connected to the second connection port 220.
Dispenser for electroporation containing disposable cartridges.
According to claim 4,
In the drug transport unit 230,
A peristaltic tubing pump is further provided to improve the flow rate precision of the injected drug when injecting the drug into the drug injector,
The peristaltic tubing pump,
Characterized in that it operates by receiving power from the power generation means 300,
Dispenser for electroporation containing disposable cartridges.
According to claim 5,
The drug injector,
When the electroporator is integrated and provided inside the electroporator, the electroporator is connected to the second connection port 220.
Dispenser for electroporation containing disposable cartridges.
According to claim 4,
In the voltage unit 400,
A plurality of connection terminals 410 are connected to the voltage generation means to receive voltage from the voltage generation means,
The control unit 500,
It is further provided with a voltage providing means (520) for transmitting the voltage applied to the voltage unit (400) to the electroporator,
The voltage providing means 520 is,
Characterized in that it is provided in a number corresponding to the number of the second connection ports 220,
Dispenser for electroporation containing disposable cartridges.
According to claim 2,
At both ends of the step surface 110,
A coupling guider 111 provided with a coupling groove 111a so that the disposable cartridge 200 can be easily coupled to the case part 100 is further provided,
On both sides 240 of the disposable cartridge 200,
Characterized in that it is further provided with a coupling protrusion 241 that corresponds to the shape of the coupling groove 111a and is coupled to the shape of the coupling groove 111a.
Dispenser for electroporation containing disposable cartridges.
According to claim 8,
The control unit 500,
Characterized in that it is further provided with an emergency stop means (530) that immediately stops the operation of the power generation means (300) and the voltage unit (400) to respond to an emergency situation that may occur during the procedure.
Dispenser for electroporation containing disposable cartridges.
According to clause 9,
On the upper surface 120 of the case portion 100,
When the disposable cartridge 200 and the case portion 100 are combined, a rotational fixing member 130 is further provided to improve the holding force of the disposable cartridge 200,
The rotating fixture 130 is,
Before the disposable cartridge 200 and the case portion 100 are coupled to each other by being provided with a rotation axis 131 and a rotation means 132 that rotates around the rotation axis 131,
The rotation means 132 is fixed at the position of the upper surface 120,
When the disposable cartridge 200 and the case portion 100 are combined,
The rotating means 132 rotates in the direction of the step surface 110 so that the disposable cartridge 200 is easily fixed.
Dispenser for electroporation containing disposable cartridges.