KR20230124946A - 고-충실도 DNA 폴리머라아제를 이용한 rhPCR 기반 앰플리콘시퀀싱에서 프라이머 이량체 및 오프-타겟 증폭을 감소시키는 RNAse H2 돌연변이체 - Google Patents

고-충실도 DNA 폴리머라아제를 이용한 rhPCR 기반 앰플리콘시퀀싱에서 프라이머 이량체 및 오프-타겟 증폭을 감소시키는 RNAse H2 돌연변이체 Download PDF

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KR20230124946A
KR20230124946A KR1020237022952A KR20237022952A KR20230124946A KR 20230124946 A KR20230124946 A KR 20230124946A KR 1020237022952 A KR1020237022952 A KR 1020237022952A KR 20237022952 A KR20237022952 A KR 20237022952A KR 20230124946 A KR20230124946 A KR 20230124946A
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rnase
primer
glu
lys
ala
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조셉 도보시
존 프롤리그
캐서린 페르슈바허
크리스틴 벨츠
스캇 로즈
마크 아론 벨케
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인티그레이티드 디엔에이 테크놀로지스 아이엔씨.
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/26—Endoribonucleases producing 5'-phosphomonoesters (3.1.26)
    • C12Y301/26004—Ribonuclease H (3.1.26.4)

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  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
KR1020237022952A 2020-12-24 2021-12-22 고-충실도 DNA 폴리머라아제를 이용한 rhPCR 기반 앰플리콘시퀀싱에서 프라이머 이량체 및 오프-타겟 증폭을 감소시키는 RNAse H2 돌연변이체 Pending KR20230124946A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063130548P 2020-12-24 2020-12-24
US63/130,548 2020-12-24
US202163277273P 2021-11-09 2021-11-09
US63/277,273 2021-11-09
PCT/US2021/064879 WO2022140553A1 (en) 2020-12-24 2021-12-22 Rnase h2 mutants that reduce primer dimers and off-target amplification in rhpcr-based amplicon sequencing with high-fidelity dna polymerases

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KR20230124946A true KR20230124946A (ko) 2023-08-28

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KR1020237022952A Pending KR20230124946A (ko) 2020-12-24 2021-12-22 고-충실도 DNA 폴리머라아제를 이용한 rhPCR 기반 앰플리콘시퀀싱에서 프라이머 이량체 및 오프-타겟 증폭을 감소시키는 RNAse H2 돌연변이체

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US (2) US12024742B2 (https=)
EP (1) EP4267762B1 (https=)
JP (1) JP2024501832A (https=)
KR (1) KR20230124946A (https=)
AU (1) AU2021409723A1 (https=)
CA (1) CA3206360A1 (https=)
ES (1) ES3060434T3 (https=)
WO (1) WO2022140553A1 (https=)

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
ATE138106T1 (de) 1988-07-20 1996-06-15 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
DE60027040T2 (de) 1999-10-29 2006-11-23 Stratagene California, La Jolla Zusammensetzungen und methoden zur verwendung von dna polymerasen
US20090068643A1 (en) 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2644708B1 (en) * 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. RNASE-H-based assays utilizing modified RNA monomers
EP2333109B1 (en) * 2008-09-03 2013-08-07 Takara Bio, Inc. Composition for detection of rna
JP2016536995A (ja) * 2013-11-14 2016-12-01 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体
HK1247255A1 (zh) 2014-12-15 2018-09-21 Cepheid 底数大於2的指数核酸扩增
WO2018031625A2 (en) * 2016-08-09 2018-02-15 Integrated Dna Technologies, Inc. Rnase h mutants in an emulsion
WO2018044831A1 (en) * 2016-08-30 2018-03-08 Integrated Dna Technologies, Inc. Cleavable hairpin primers
CN112105745A (zh) * 2018-05-07 2020-12-18 罗氏创新中心哥本哈根有限公司 用于寡核苷酸治疗剂的大规模平行发现方法
JP2022515466A (ja) * 2018-12-28 2022-02-18 ナショナル ユニヴァーシティー オブ シンガポール 標的化された相補的ｄｎａ富化のための方法

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WO2022140553A1 (en) 2022-06-30
AU2021409723A9 (en) 2024-09-12
JP2024501832A (ja) 2024-01-16
US20220348997A1 (en) 2022-11-03
CA3206360A1 (en) 2022-06-30
EP4267762B1 (en) 2025-09-17
US20240318237A1 (en) 2024-09-26
AU2021409723A1 (en) 2023-06-22
EP4267762C0 (en) 2025-09-17
US12024742B2 (en) 2024-07-02
ES3060434T3 (en) 2026-03-26
EP4267762A1 (en) 2023-11-01

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