KR20230115546A - Bomb type cosmetic composition containing cinnamon extract and method for preparing same - Google Patents

Abstract

The present invention relates to a balm-type cosmetic composition containing a cinnamon extract and a method for producing the same, and more specifically to a cosmetic composition in the form of a green balm containing a cinnamon extract for the treatment, relief and prevention of athlete's foot, and a method for producing the same. The night-type cosmetic composition containing the cinnamon extract of the present invention is effective in treating, curing, and preventing athlete's foot. In addition, the present invention has the advantage that there is no side effect as it uses a natural product as a raw material, and it is made portable and easy to use.

Description

Bomb type cosmetic composition containing cinnamon extract and method for preparing same {Bomb type cosmetic composition containing cinnamon extract and method for preparing same}

The present invention relates to a balm-type cosmetic composition containing a cinnamon extract and a method for producing the same, and more specifically to a cosmetic composition in the form of a green balm containing a cinnamon extract for the treatment, relief and prevention of athlete's foot, and a method for producing the same.

Cinnamon is the dried outer skin of the cinnamon tree (Cinnamomum cassia), and cinnamon oil is an essential oil extracted from the bark or leaves of the cinnamon tree belonging to the genus Cinnamomum, which is mainly distributed in Southeast Asia, China, and India. Cinnamon essential oil is divided into bark oil extracted from the bark of a tree and leaf oil extracted from its leaves. The main component of bark oil is mostly trans-cinnamaldehyde, a monoterpene compound (-CHO) of the aldehyde family. Cinnamon essential oil and trans-cinnamaldehyde are known as natural insecticides that have little side effects on the human body and the environment because they have no resistance to the mixture of biologically active substances and have selective insecticidal and acaricidal effects only on target organisms. . In addition, the above ingredients are also used in the medical field as natural antibacterial substances against strains that cause respiratory or skin diseases.

Dermatophytes, which are fungi, invade the stratum corneum of the skin, hair, fingernails and toenails to cause skin fungal infection, and Trichophyton mentagrophytes is known to cause athlete's foot as a representative causative agent of superficial mycosis. Antifungal agents are used to treat athlete's foot, and are suitable for treatment as they consist of steroids, immunosuppressants, antibiotics and synthetic chemicals. However, it is known to be accompanied by various side effects such as strong irritation or residual toxicity, and as the occurrence of resistant bacteria is increasing due to the misuse and abuse of antibiotics recently, the development of resistant bacteria treatment is also on the rise. As an alternative to these problems, the search for natural materials derived from plants has been attempted, and the antifungal effect is being investigated in various plant groups. In addition, various natural substances and physiologically active substances contained in plants have fewer side effects or residual toxicity than synthetic compounds, and thus, studies are being actively conducted to use them as medicine. In addition, there is a problem in that commercially available ointments are used 2 to 3 times a day or more, and athlete's foot medications must be used for a relatively long period of time, from 6 weeks to 3 months.

In order to solve this problem, the inventors of the present invention completed the present invention by preparing a night-type cosmetic composition for easy and convenient management of athlete's foot using a cinnamon extract having antifungal and anti-inflammatory effects.

Republic of Korea Patent Publication No. 10-2021-0000621

In order to solve the problems described above, an object of the present invention is to provide a night-type cosmetic composition containing a cinnamon extract for the treatment, relief and prevention of athlete's foot.

In order to achieve the above object, the present invention discloses a night-type cosmetic composition comprising a cinnamon extract and a cosmetic powder.

Here, the cinnamon extract may include cinnamon essential oil and trans-cinnamaldehyde as main components.

Here, the cinnamon essential oil may have a concentration within the range of 1.0 to 10.0 μg/ml.

On the other hand, the present invention comprises the steps of mixing and stirring the cinnamon extract and cosmetic powder in a solvent; compressing the stirred mixture to produce a solid form; And drying the resulting solid shape to prepare a night-type cosmetic composition; containing, a method for producing a night-type cosmetic composition is further disclosed.

Here, the cinnamon extract may include cinnamon essential oil and trans-cinnamaldehyde as main components.

Here, the cinnamon essential oil may have a concentration within the range of 1.0 to 10.0 μg/ml.

The night-type cosmetic composition containing the cinnamon extract of the present invention according to the above is effective in treating, curing, and preventing athlete's foot. In addition, the present invention has the advantage that there is no side effect as it uses a natural product as a raw material, and it is made portable and easy to use.

1 is a graph showing the MTT assay results of cinnamon essential oil and trans-cinnamaldehyde for HaCaT.
Figure 2 is a graph showing the MTT assay results of cinnamon essential oil and trans-cinnamaldehyde for RAW264.7.
3 is a graph showing the results of anti-inflammatory effect experiments of cinnamon essential oil and trans-cinnamaldehyde.
4 is a photograph of the results of measuring the antibacterial activity of green balm to which cinnamon essential oil was added against E. coli and S. aureus .
Figure 5 is a photograph of the manufacturing state of the balm-type cosmetic composition of the present invention.
Figure 6 is a photograph of the finished product in the form of foot balm according to the manufacturing method of the balm-type cosmetic composition of the present invention.

Terms used in this application are only used to describe specific examples. Therefore, for example, expressions in the singular number include plural expressions unless the context clearly requires them to be singular. In addition, the terms "include" or "have" used in this application are used to clearly indicate that the features, steps, functions, components, or combinations thereof described in the specification exist, but other features It should be noted that it is not intended to be used to preliminarily exclude the presence of any steps, functions, components, or combinations thereof.

Meanwhile, unless otherwise defined, all terms used in this specification should be regarded as having the same meaning as commonly understood by a person of ordinary skill in the art to which the present invention belongs. Accordingly, certain terms should not be interpreted in an overly idealistic or formal sense unless clearly defined herein.

As a result of research in order to solve the above-mentioned problems, the inventors of the present invention came up with the following invention. The present specification discloses a night-type cosmetic composition containing a cinnamon extract and a cosmetic powder.

In the night-type cosmetic composition of the present invention, the cinnamon extract may include cinnamon essential oil and trans-cinnamaldehyde as main components.

In the night-type cosmetic composition of the present invention, the cinnamon essential oil preferably has a concentration within the range of 1.0 to 10.0 μg/ml, and more preferably has a concentration of 5.0 μg/ml.

In the balm-type cosmetic composition of the present invention, the cosmetic powder means a cosmetic powder that can be appropriately employed from the point of view of a person skilled in the art, and may include components commonly used in balm-type cosmetic compositions.

On the other hand, the present specification comprises the steps of mixing and stirring the cinnamon extract and cosmetic powder in a solvent; compressing the stirred mixture to produce a solid form; And drying the resulting solid shape to prepare a night-type cosmetic composition; containing, a method for producing a night-type cosmetic composition is further disclosed.

In the manufacturing method of the night-type cosmetic composition of the present invention, the cinnamon extract may contain cinnamon essential oil and trans-cinnamaldehyde as main components.

In the manufacturing method of the night-type cosmetic composition of the present invention, the cinnamon essential oil preferably has a concentration within the range of 1.0 to 10.0 μg/ml, and more preferably has a concentration of 5.0 μg/ml.

In the manufacturing method of the balm-type cosmetic composition of the present invention, the cosmetic powder means a cosmetic powder that can be appropriately employed from the point of view of a person skilled in the art, and may include components commonly used in balm-type cosmetic compositions.

In the step of stirring in the preparation method of the balm-type cosmetic composition of the present invention, it is preferable to stir for 10 to 30 minutes, but is not limited thereto.

In addition, the solvent for stirring in the preparation method of the balm-type cosmetic composition of the present invention is preferably an organic solvent such as dimethyl sulfoxide (DMSO), but is not limited thereto.

In addition, in the manufacturing method of the balm-type cosmetic composition of the present invention, it may be possible to compress and design the powder using a design mold to create a solid shape.

In addition, the time for drying the solid form in the manufacturing method of the balm-type cosmetic composition of the present invention is preferably one within 24 to 48 hours, but is not limited thereto.

Hereinafter, with reference to the accompanying drawings and embodiments will be described in more detail with respect to what the present specification claims. However, the drawings or embodiments presented in this specification can be modified in various ways by those skilled in the art and have various forms, and the description in this specification is not limited to the specific disclosure form of the present invention. It should be regarded as including all equivalents or substitutes included in the spirit and technical scope of the present invention. In addition, the accompanying drawings are presented to help those skilled in the art to more accurately understand the present invention, and may be exaggerated or reduced than actual.

{Example and evaluation}

Cell test (MTT assay, anti-inflammatory test (NO assay))

HaCaT cells and RAW264.7 (1x10 ⁵ cells/well) to be used in cell experiments were subcultured and activated every 2 to 3 days. If sufficiently active, diluted to 1x10 ⁶ cells/ml in a 96 well plate, seeded by 100 μl per well, and cultured for 24 hours in a cell incubator maintained at 37°C and 5% CO ₂ conditions. Cinnamon essential oil and trans-cinnamaldehyde used as samples were dissolved in 100% DMSO at 0.20 g/ml, respectively, and then 200, 100, 50, 25, 10, 5, 2.5, It was diluted with DMEM medium supplemented with 10% fetal bovine serum and 1% penicillin to a concentration of 1, 0.5 μg/ml. At this time, the DMSO concentration of all samples was finally set to 1%. After 24 hours of incubation, the samples were treated by concentration and re-cultured for 24 hours under the same conditions. In the case of RAW264.7, after adding LPS for NO assay, it was treated with the sample using DMEM medium. After sample treatment, the concentration of LPS was set to 500 ng/ml. After 24 hours, RAW264.7 took 100 μl of the supernatant from each well, transferred it to a 96 well plate, and added the same amount of Griess reagent [1% sulfanilamide, 0.1% N-(1-naphtyl)-ethylene diamine dihydrochloride in 2.5% phosphoric acid]. After mixing, the absorbance was measured at 540 nm with an ELISA reader. As a positive control, L-NAME (250 μM) was used as an inhibitor that inhibits the production of NO by inhibiting the activity of iNOS. Then, the solution remaining in the 96 well plate was suctioned off, treated with MTT [3-[4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide] reagent, and after about 4 hours, the absorbance was measured with an ELISA reader at 550 nm. measured. The NO assay and MTT assay of RAW264.7 and the MTT assay of HaCaT cell yielded concentrations that were non-toxic and had anti-inflammatory effects.

antibacterial experiment

Antibacterial experiments were conducted by paper disk diffusion. Representative gram-negative bacteria, E. coli , and gram-positive bacteria, S. aureus , were used in the experiment. Each germ was pre-cultured once a day, and the paper disk used in the experiment was sterilized at high temperature and high pressure in an autoclave. Bacteria were cultured in LB medium, and turbidity was measured using a UV-vis spectrophotometer before the experiment. When the wavelength value at 600 nm was 0.1, the approximate number of bacteria was calculated assuming 1 x 10 ⁸ CFU/ml. Since the paper disk diffusion method smears almost no bacterial colony, 200 μl of 1 x 10 ⁸ CFU/ml for E. coli and 1 x 10 ⁷ CFU/ml for S. aureus was spread on LB solid medium. Cinnamon essential oil is non-aqueous, and 0.140 g of a solid powder uniformly mixed with citric acid, corn starch, and sodium bicarbonate and 0.16 μl of cocobetaine, an amphoteric surfactant, were dissolved in 20 ml of distilled water to adjust the concentration to the desired concentration. As a negative control, a solvent in which the sample was dissolved was used, and as a positive control, 1 mg/ml of ampicillin, a well-known antibacterial agent, was used. After dispensing 50 μl of all samples on an 8 nm paper disk, they were placed on LB solid medium smeared with bacteria with sterilized tweezers and incubated for about 16 hours in a 35 ° C incubator. Then, the diameter of the generated clear zone was measured to compare the antibacterial activity.

Cell test results and evaluation

MTT assay is the most widely used method to evaluate cell activity, that is, changes in cell growth and apoptosis, for various food bioactive substances. It is a reduction method using diphenyltetrazolium bromide reagent. This reduction method using the MTT reagent uses the principle that when cells are treated with yellow water-soluble MTT tetrazolium, the ring structure of tetrazolium is broken by dehydrogenase in the mitochondria of living cells and reduced to blue-violet water-insoluble MTT formazan. MTT formazan is generally dissolved in an organic solvent such as dimethyl sulfoxide (DMSO) to measure absorbance. At this time, the maximum absorbance of formazan dye is around 540 nm, and living cells can be quantitatively evaluated according to the measured absorbance value.

The inflammatory response is a bioprotective reaction that restores damaged areas due to physical and chemical stimuli from the outside. When the stimulation is applied, vasoactive substances are released locally, vascular permeability increases, and an immune response is induced. Macrophages are distributed in all tissues in the body of animals and play a major role in maintaining defense and homeostasis in the human immune system. It is known that nitric oxide (NO), a kind of active free species in the body's inflammatory process, is produced by immune cells such as macrophages and plays an important role in various physiological and pathological processes. NO is a very unstable compound and is produced through the activation of iNOS, an NO synthase, by inflammatory substances such as LPS and TNF-α. Therefore, it is important to inhibit the activity of iNOS for the inhibition and treatment of inflammation. NO assay measured the amount of NO produced using Griess reagent [1% sulfanilamide, 0.1% N-(1-naphtyl)-ethylene diamine dihydrochloride in 2.5% phosphoric acid], and RAW264.7 (1x10 ⁵ cells/well) LPS was treated with samples by concentration and reacted for 24 hours. 100 μl of medium for each experimental group and griess reagent were mixed in equal amounts and absorbance was measured at a wavelength of 540 nm. Nitric oxide production was calculated by preparing a calibration curve using sodium nitrate (NaNO ₃ ) as a standard. As a positive control, L-NAME [N-Nitroarginine methyl ester] was used as an inhibitor that inhibits the production of NO by inhibiting the activity of iNOS.

1 is a graph showing the MTT assay results of cinnamon essential oil and trans-cinnamaldehyde for HaCaT. 2 is a graph showing the MTT assay results of cinnamon essential oil and trans-cinnamaldehyde for RAW264.7. In addition, Figure 3 is a graph showing the results of the anti-inflammatory effect test of cinnamon essential oil and trans-cinnamaldehyde.

1 to 3, the MTT assay results for HaCaT, which are skin keratinocytes, show that the cell viability of cinnamon essential oil and trans-cinnamaldehyde with a final concentration of 0.05 to 20 μg/ml is mostly around 100% in the entire range. It can be confirmed that there is no toxicity throughout. Furthermore, RAW264.7 was found to be nontoxic at most concentrations, but the cell viability of 20 μg/ml, a high concentration sample of trans-cinamaldehyde, was 76%, suggesting that 20 μg/ml of trans-cinamaldehyde In the case of , it can be confirmed that it is relatively toxic.

In addition, referring to FIGS. 1 to 3, as a result of the NO assay of RAW264.7, NO was not produced in the control − not treated with LPS, but iNOS was expressed by LPS stimulation in the control + treated with LPS, and about 30 μM It can be confirmed that NO of is generated. Furthermore, L-NAME, a well-known anti-inflammatory agent used as a positive control, inhibits iNOS expression, and finally, it can be seen that 16.31 μM of NO, which is about half of that of control+, was produced.

Cinnamon essential oil showed no significant change from 0.05 to 0.5 μg/ml, which is a low concentration, but from 1.0 μg/ml to 20 μg/ml, the amount of NO produced decreased in a concentration-dependent manner. can It can be seen that trans-cinamaldehyde also shows a similar pattern and decreases NO in a concentration-dependent manner from 0.5 μg/ml to 20 μg/ml. However, at 20 μg / ml, it was significantly reduced compared to the previous concentration, which is estimated to be related to the toxicity at 20 μg / ml as a result of the MTT assay of RAW264.7.

Based on this, it was added to the product in a concentration range of 0.5 to 10.0 μg/ml, which is a concentration range that does not affect the skin, and the antibacterial activity against E. coli and S. aureus was investigated by the paper disk diffusion method.

Antibacterial test results and evaluation

4 is a photograph of the results of measuring the antibacterial activity of foot balm to which cinnamon essential oil was added against E. coli and S. aureus . Here, a is solvent (control), b is ampicillin 1 mg/ml (positive control), c is cinnamon essential oil 0.5 μg/ml, d is cinnamon essential oil 1.0 μg/ml, e is cinnamon essential oil 2.5 μg/ml, f is cinnamon essential oil 5.0 μg/ml and g is cinnamon essential oil 10.0 μg/ml.

Table 1 shows the results of measuring the antibacterial activity of ampicillin and cinnamon essential oil against E. coli and S. aureus .

Referring to FIG. 4 and Table 1, the positive control for E. coli exhibited a diameter increased by 1.4 cm compared to the negative control, and a diameter similar to that of the positive control appeared from a sample concentration of 25 μg / ml. I was able to confirm. As for S. aureus , the diameter of the positive control was increased by 2.6 cm, and at a concentration of 5.0 μg/ml, it was also increased by 2.6 cm, indicating that the antibacterial activity reached to ampicillin at a concentration of 5.0 μg/ml or more. Therefore, within 1.0 to 10.0 μg / ml, it can be seen that the night-type cosmetic composition to which cinnamon essential oil is added is not toxic to the skin and has excellent anti-inflammatory and antibacterial effects.

Figure 5 is a photograph of the manufacturing state of the balm-type cosmetic composition of the present invention. In addition, Figure 6 is a photograph of the finished product in the form of foot balm according to the manufacturing method of the balm-type cosmetic composition of the present invention. As shown in FIGS. 5 and 6, the present inventors prepared a cosmetic composition in the form of a foot balm to which the concentration of cinnamon essential oil was added at 5 μg/ml as a final product.

The night-type cosmetic composition containing the cinnamon extract of the present invention according to the above is effective in treating, curing, and preventing athlete's foot. In addition, the present invention has the advantage that there is no side effect as it uses a natural product as a raw material, and it is made portable and easy to use.

The above description is merely an example of the technical idea of the present invention, and various modifications and variations can be made to those skilled in the art without departing from the essential characteristics of the present invention.

Therefore, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but to explain, and the scope of the technical idea of the present invention is not limited by these embodiments. The protection scope of the present invention should be construed according to the claims below, and all technical ideas within the equivalent range should be construed as being included in the scope of the present invention.

Claims (6)

A night-type cosmetic composition containing a cinnamon extract and cosmetic powder.
According to claim 1,
The cinnamon extract is a night-type cosmetic composition containing cinnamon essential oil and trans-cinnamaldehyde as main components.
According to claim 1,
The cinnamon essential oil is a night-type cosmetic composition, characterized in that it has a concentration within the range of 1.0 to 10.0 μg / ml.
Stirring the mixture of cinnamon extract and cosmetic powder in a solvent;
compressing the stirred mixture to produce a solid form; and
Method for producing a night-type cosmetic composition comprising a; step of preparing a night-type cosmetic composition by drying the resulting solid shape.
According to claim 4,
The method of producing a night-type cosmetic composition, wherein the cinnamon extract contains cinnamon essential oil and trans-cinnamaldehyde as main components.
According to claim 4,
The cinnamon essential oil is characterized in that it has a concentration within the range of 1.0 to 10.0 ㎍ / ml, a method for producing a night-type cosmetic composition.