KR20230074207A - 카고 뉴클레오타이드 서열을 전위시키기 위한 시스템 및 방법 - Google Patents

카고 뉴클레오타이드 서열을 전위시키기 위한 시스템 및 방법 Download PDF

Info

Publication number
KR20230074207A
KR20230074207A KR1020237013324A KR20237013324A KR20230074207A KR 20230074207 A KR20230074207 A KR 20230074207A KR 1020237013324 A KR1020237013324 A KR 1020237013324A KR 20237013324 A KR20237013324 A KR 20237013324A KR 20230074207 A KR20230074207 A KR 20230074207A
Authority
KR
South Korea
Prior art keywords
leu
lys
sequence
glu
arg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020237013324A
Other languages
English (en)
Korean (ko)
Inventor
브라이언 토마스
크리스토퍼 브라운
다니엘라 에스 에이 골츠만
크리스티나 버터필드
리사 알렉산더
제이슨 리우
Original Assignee
메타지노미, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 메타지노미, 인크. filed Critical 메타지노미, 인크.
Publication of KR20230074207A publication Critical patent/KR20230074207A/ko
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
KR1020237013324A 2020-09-24 2021-08-23 카고 뉴클레오타이드 서열을 전위시키기 위한 시스템 및 방법 Pending KR20230074207A (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202063082983P 2020-09-24 2020-09-24
US63/082,983 2020-09-24
US202163187290P 2021-05-11 2021-05-11
US63/187,290 2021-05-11
US202163232578P 2021-08-12 2021-08-12
US63/232,578 2021-08-12
PCT/US2021/047196 WO2022066335A1 (en) 2020-09-24 2021-08-23 Systems and methods for transposing cargo nucleotide sequences

Publications (1)

Publication Number Publication Date
KR20230074207A true KR20230074207A (ko) 2023-05-26

Family

ID=80846851

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020237013324A Pending KR20230074207A (ko) 2020-09-24 2021-08-23 카고 뉴클레오타이드 서열을 전위시키기 위한 시스템 및 방법

Country Status (8)

Country Link
US (1) US20230340481A1 (https=)
EP (1) EP4217499A4 (https=)
JP (1) JP2023542976A (https=)
KR (1) KR20230074207A (https=)
AU (1) AU2021350637A1 (https=)
CA (1) CA3192927A1 (https=)
MX (1) MX2023003436A (https=)
WO (1) WO2022066335A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024055013A1 (en) * 2022-09-08 2024-03-14 Metagenomi, Inc. Systems and methods for transposing cargo nucleotide sequences
WO2024055012A1 (en) * 2022-09-08 2024-03-14 Metagenomi, Inc. Systems and methods for transposing cargo nucleotide sequences
WO2024187119A2 (en) * 2023-03-08 2024-09-12 Metagenomi, Inc. Systems and methods for transposing cargo nucleotide sequences
WO2024233984A2 (en) * 2023-05-10 2024-11-14 Metagenomi, Inc. Systems and methods for transposing cargo nucleotide sequences
WO2025250465A1 (en) * 2024-05-28 2025-12-04 Nanjing Probio Biotech Co., Ltd. Vector composition and use thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201514510D0 (en) * 2015-08-14 2015-09-30 Nemesis Bioscience Ltd Delivery vehicle
CA3124110A1 (en) * 2018-12-17 2020-06-25 The Broad Institute, Inc. Crispr-associated transposase systems and methods of use thereof
CA3132197A1 (en) * 2019-03-07 2020-09-10 The Trustees Of Columbia University In The City Of New York Rna-guided dna integration using tn7-like transposons

Also Published As

Publication number Publication date
EP4217499A4 (en) 2025-02-12
JP2023542976A (ja) 2023-10-12
MX2023003436A (es) 2023-04-14
CA3192927A1 (en) 2022-03-31
EP4217499A1 (en) 2023-08-02
WO2022066335A1 (en) 2022-03-31
US20230340481A1 (en) 2023-10-26
AU2021350637A1 (en) 2023-06-01
AU2021350637A9 (en) 2024-09-19

Similar Documents

Publication Publication Date Title
CA3130135C (en) Enzymes with RuVC domains
US12024727B2 (en) Enzymes with RuvC domains
US20230340481A1 (en) Systems and methods for transposing cargo nucleotide sequences
US20220298494A1 (en) Enzymes with ruvc domains
WO2021178934A1 (en) Class ii, type v crispr systems
EP4165177A1 (en) Enzymes with ruvc domains
US20220220460A1 (en) Enzymes with ruvc domains
US20240301374A1 (en) Systems and methods for transposing cargo nucleotide sequences
CN116615547A (zh) 用于对货物核苷酸序列转座的系统和方法
KR20240145512A (ko) 융합 단백질
GB2617659A (en) Enzymes with RUVC domains

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20230419

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20240816

Comment text: Request for Examination of Application