KR20230069404A - Device for extracting nucleic acids - Google Patents

Device for extracting nucleic acids Download PDF

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KR20230069404A
KR20230069404A KR1020210155407A KR20210155407A KR20230069404A KR 20230069404 A KR20230069404 A KR 20230069404A KR 1020210155407 A KR1020210155407 A KR 1020210155407A KR 20210155407 A KR20210155407 A KR 20210155407A KR 20230069404 A KR20230069404 A KR 20230069404A
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nucleic acid
magnetic material
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김종진
김철
이원주
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한국전기연구원
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    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

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Abstract

본 발명은 핵산 추출 및 증폭에 관한 것으로서, 보다 상세하게는 검체로부터 핵산을 추출하는 핵산 추출 장치에 관한 것이다.
본 발명은, 추출된 검체로부터 자성물질에 결합시켜 핵산을 추출하는 핵산추출부(100)와; 상기 핵산추출부(100)로부터 핵산이 결합된 자성물질이 포함된 혼합액을 전달받아 핵산이 결합된 자성물질을 남기고 혼합액을 배출하는 추출용 버퍼부(200)와; 일단에 상기 추출용 버퍼부(200)와 연결되어 증폭 시약이 담인 하나 이상의 증폭챔버(310)로 상기 추출용 버퍼부(200)로부터 핵산이 결합된 자성물질을 전달하는 전달유로(450)를 포함하는 것을 특징으로 하는 핵산 추출 장치를 개시한다.The present invention relates to nucleic acid extraction and amplification, and more particularly, to a nucleic acid extraction device for extracting nucleic acids from a specimen.
The present invention, a nucleic acid extraction unit (100) for extracting nucleic acids by binding to a magnetic material from the extracted specimen; an extraction buffer unit 200 for receiving the mixed liquid containing the magnetic material to which the nucleic acid is coupled from the nucleic acid extracting unit 100 and discharging the mixed liquid while leaving the magnetic material to which the nucleic acid is bound; At one end, a transfer passage 450 is connected to the extraction buffer unit 200 and transfers the magnetic material to which the nucleic acid is bound from the extraction buffer unit 200 to one or more amplification chambers 310 containing amplification reagents. Disclosed is a nucleic acid extraction device characterized in that.

Description

핵산 추출 장치 {Device for extracting nucleic acids}Nucleic acid extraction device {Device for extracting nucleic acids}

본 발명은 핵산 추출 및 증폭에 관한 것으로서, 보다 상세하게는 검체로부터 핵산을 추출하는 핵산 추출 장치에 관한 것이다.The present invention relates to nucleic acid extraction and amplification, and more particularly, to a nucleic acid extraction device for extracting nucleic acids from a specimen.

코로나 바이러스 분자진단은, 현장에서 직접 검사가 어렵기 때문에 채취된 환자의 검체를 검사기관으로 이송하는 과정이 필수적이다.For molecular diagnosis of coronavirus, it is difficult to test directly on the spot, so the process of transferring the collected patient's sample to the testing institution is essential.

검체 이송 후 핵산 추출 과정 및 핵산 증폭 검사(RT-PCR)를 통해 시료 내 바이러스 검출 여부를 확인하는데, 최소 6시간 정도 소요된다.After transferring the sample, it takes at least 6 hours to check whether the virus is detected in the sample through the nucleic acid extraction process and nucleic acid amplification test (RT-PCR).

이에 신속진단을 위해서는 현장에서 적용 가능한 핵산 추출 및 핵산 증폭 시스템 개발이 필수적이다.Therefore, for rapid diagnosis, it is essential to develop a nucleic acid extraction and nucleic acid amplification system that can be applied in the field.

그러나 기존 핵산추출 과정은 최소 7단계로 구성되어 매우 복잡하여 자동화 시스템 규모가 커서 현장에 적용하기에 용이하지 않은 문제점이 있다.However, the existing nucleic acid extraction process consists of at least 7 steps and is very complicated, so it is not easy to apply it to the field due to the large size of the automated system.

한편, 핵산 추출 및 증폭을 자동으로 수행하는 자동화 시스템(Gene X pert, Film Array 등)이 Cepheid, Biomerieux 사 등에 의하여 상용화되고 있다.Meanwhile, automated systems (Gene X pert, Film Array, etc.) that automatically perform nucleic acid extraction and amplification are being commercialized by Cepheid, Biomerieux, and the like.

위 두 회사의 자동화 시스템은, 유사한 방법에 의하여 수행되며, 구체적으로 샘플 카트리지와, 샘플 카트리지 내 시료 이송이 자동으로 가능한 시스템으로 구성된다.The automated systems of the above two companies are performed in a similar manner, and are specifically composed of a sample cartridge and a system capable of automatically transporting samples within the sample cartridge.

그런데 1회 검사에 최소 50~120$ 정도의 비용이 발생하여 현장용으로 사용하기에는 매우 고가인 문제점이 있다.However, there is a problem in that it costs at least 50 to 120 dollars for one inspection, which is very expensive to use for field use.

더 나아가 하나의 카트리지는 하나의 샘플 처리만 가능하기 때문에, 검사 결과 분석에 필요한 Positive control, Negative control을 동시에 분석하기 어렵다는 문제를 가지고 있다.Furthermore, since one cartridge can only process one sample, it is difficult to simultaneously analyze the positive control and negative control required for analysis of test results.

(특허문헌 1) KR 10-2015-0127917 A(Patent Document 1) KR 10-2015-0127917 A

본 발명의 목적은, 핵산이 결합된 자성물질을 간단한 구조에 의하여 추출하는 추출용 버퍼공간을 구비하여 핵산추출을 신속하게 수행할 수 있는 핵산 추출 장치를 제공하는데 있다.An object of the present invention is to provide a nucleic acid extracting apparatus capable of rapidly extracting nucleic acids by having an extraction buffer space for extracting a magnetic material to which nucleic acids are bound by a simple structure.

본 발명은 상기와 같은 본 발명의 목적을 달성하기 위하여 창출된 것으로서, 본 발명은, 추출된 검체로부터 자성물질에 결합시켜 핵산을 추출하는 핵산추출부(100)와; 상기 핵산추출부(100)로부터 핵산이 결합된 자성물질이 포함된 혼합액을 전달받아 핵산이 결합된 자성물질을 남기고 혼합액을 배출하는 추출용 버퍼부(200)와; 일단에 상기 추출용 버퍼부(200)와 연결되어 증폭 시약이 담인 하나 이상의 증폭챔버(310)로 상기 추출용 버퍼부(200)로부터 핵산이 결합된 자성물질을 전달하는 전달유로(450)를 포함하는 것을 특징으로 하는 핵산 추출 장치를 개시한다.The present invention was created to achieve the object of the present invention as described above, and the present invention includes a nucleic acid extraction unit 100 for extracting nucleic acids by binding to a magnetic material from an extracted specimen; an extraction buffer unit 200 for receiving the mixed liquid containing the magnetic material to which the nucleic acid is coupled from the nucleic acid extracting unit 100 and discharging the mixed liquid while leaving the magnetic material to which the nucleic acid is coupled; At one end, a transfer passage 450 is connected to the extraction buffer unit 200 and transfers the magnetic material to which the nucleic acid is bound from the extraction buffer unit 200 to one or more amplification chambers 310 containing amplification reagents. Disclosed is a nucleic acid extraction device characterized in that.

상기 전달유로(450) 내 음압을 형성하여 상기 추출용 버퍼부(200)로부터 핵산이 결합된 자성물질의 이동을 유도하는 음압형성부(430)가 타단에 형성될 수 있다.A negative pressure forming unit 430 may be formed at the other end of the transmission passage 450 to induce movement of the magnetic material to which the nucleic acid is coupled from the extraction buffer unit 200 by forming a negative pressure.

상기 추출용 버퍼부(200)는, 상기 핵산추출부(100)로부터 핵산이 결합된 자성물질이 포함된 혼합액이 임시로 담기는 버퍼용기(210)와; 상기 버퍼용기(210)에 결합되어 자력에 의하여 핵산이 결합된 자성물질을 포집하는 자력작용부(220)를 포함할 수 있다.The extraction buffer unit 200 includes a buffer container 210 in which a liquid mixture containing a magnetic material to which nucleic acids are bound from the nucleic acid extraction unit 100 is temporarily contained; It may include a magnetic force action unit 220 that is coupled to the buffer container 210 and collects the magnetic material to which the nucleic acid is bound by magnetic force.

상기 버퍼용기(210)는, 핵산이 결합된 자성물질을 제외한 나머지 물질을 배출하는 배출부(420)가 저면에 결합되고, 상기 전달유로(450)는, 상기 버퍼용기(210)의 바닥보다 낮게 연결될 수 있다.In the buffer container 210, a discharge part 420 for discharging materials other than the magnetic material to which nucleic acids are bound is coupled to a bottom surface, and the transmission passage 450 is lower than the bottom of the buffer container 210. can be connected

상기 전달유로(450)의 일단에는, 상기 전달유로(450)를 세정하기 위한 정제수의 공급을 위한 제4밸브(460)가 결합될 수 있다.A fourth valve 460 for supplying purified water for cleaning the transmission passage 450 may be coupled to one end of the transmission passage 450 .

본 발명에 따른 핵산 추출 장치는, 핵산이 결합된 자성물질을 간단한 구조에 의하여 추출하는 추출용 버퍼공간을 구비하여 핵산추출을 신속하게 수행할 수 있는 이점이 있다.The nucleic acid extracting apparatus according to the present invention has an advantage in that nucleic acid extraction can be quickly performed by providing a buffer space for extraction in which a magnetic material to which nucleic acids are bound is extracted by a simple structure.

또한, 본 발명에 따른 핵산 추출 장치는, 추출용 버퍼공간에서 핵산이 결합된 자성물질의 추출한 후 음압에 의하여 자성물질의 이동을 유도하여 증폭시약이 담긴 증폭시약이 담긴 챔버로 안정적으로 전달할 수 있다.In addition, the nucleic acid extracting apparatus according to the present invention extracts the magnetic material to which the nucleic acid is bound from the extraction buffer space, and then induces the movement of the magnetic material by negative pressure to stably transfer it to the chamber containing the amplification reagent. .

도 1은, 본 발명에 따른 핵산 추출 장치의 개념을 보여주는 개념도이다.
도 2는, 도 1의 핵산 추출 장치에서 추출용 버퍼부를 보여주는 개념도이다.1 is a conceptual diagram showing the concept of a nucleic acid extracting apparatus according to the present invention.
2 is a conceptual diagram showing a buffer for extraction in the nucleic acid extracting apparatus of FIG. 1;

이하 본 발명에 따른 핵산 추출 장치에 관하여 첨부된 도면을 참조하여 설명하다.Hereinafter, a nucleic acid extracting apparatus according to the present invention will be described with reference to the accompanying drawings.

본 발명에 따른 핵산 추출 장치는, 도 1 및 도 2에 도시된 바와 같이, 추출된 검체로부터 자성물질에 결합시켜 핵산을 추출하는 핵산추출부(100)와; 상기 핵산추출부(100)로부터 핵산이 결합된 자성물질이 포함된 혼합액을 전달받아 핵산이 결합된 자성물질을 남기고 혼합액을 배출하는 추출용 버퍼부(200)와; 일단에 상기 추출용 버퍼부(200)와 연결되어 증폭 시약이 담인 하나 이상의 증폭챔버(310)로 상기 추출용 버퍼부(200)로부터 핵산이 결합된 자성물질을 전달하는 전달유로(450)를 포함한다.A nucleic acid extracting apparatus according to the present invention, as shown in FIGS. 1 and 2, includes a nucleic acid extracting unit 100 that extracts nucleic acids by binding to a magnetic material from an extracted specimen; an extraction buffer unit 200 for receiving the mixed liquid containing the magnetic material to which the nucleic acid is coupled from the nucleic acid extracting unit 100 and discharging the mixed liquid while leaving the magnetic material to which the nucleic acid is coupled; At one end, a transfer passage 450 is connected to the extraction buffer unit 200 and transfers the magnetic material to which the nucleic acid is bound from the extraction buffer unit 200 to one or more amplification chambers 310 containing amplification reagents. do.

상기 핵산추출부(100)는, 추출된 검체로부터 자성물질에 결합시켜 핵산을 추출하는 구성으로서, 핵산추출을 위한 구성이면 어떠한 구성도 가능하다.The nucleic acid extraction unit 100 is a component that extracts nucleic acids by binding to a magnetic material from the extracted specimen, and any configuration is possible as long as it is configured for nucleic acid extraction.

예로서, 상기 핵산추출부(100)는, 검체로부터 핵산의 추출을 위하여 자성물질들이 담긴 혼합액이 담기는 용기로서 구성될 수 있다.For example, the nucleic acid extraction unit 100 may be configured as a container containing a liquid mixture containing magnetic materials for extraction of nucleic acids from a specimen.

그리고 상기 핵산추출부(100)는, 핵산이 자성물질에 결합되는 것을 촉진하기 위하여 미리 설정된 온도로 가열하기 위한 히터가 설치될 수 있다.In addition, the nucleic acid extracting unit 100 may be provided with a heater for heating to a preset temperature in order to promote binding of the nucleic acid to the magnetic material.

한편, 자성물질은, 핵산이 결합되도록 자성을 띠는 물질로서, 미세 입도를 가지는 자성비드들로 이루어질 수 있다.On the other hand, the magnetic material is a material that is magnetic so as to bind nucleic acids, and may be made of magnetic beads having a fine particle size.

그리고 상기 혼합액은, 검체로부터 핵산이 추출되어 자성물질에 결합될 수 있도록 촉진하는 애게로서, 추출된 핵산의 종류에 따라서 다양한 조성을 가질 수 있다.In addition, the mixed solution promotes nucleic acid extraction from a specimen and binding to a magnetic material, and may have various compositions depending on the type of extracted nucleic acid.

상기 추출용 버퍼부(200)는, 상기 핵산추출부(100)로부터 핵산이 결합된 자성물질이 포함된 혼합액을 전달받아 핵산이 결합된 자성물질을 남기고 혼합액을 배출하는 구성으로서, 다양한 구성이 가능하다.The extraction buffer unit 200 receives the mixed solution containing the magnetic material to which the nucleic acid is coupled from the nucleic acid extraction unit 100 and discharges the mixed solution leaving the magnetic material to which the nucleic acid is coupled, and various configurations are possible. do.

예로서, 상기 추출용 버퍼부(200)는, 상기 핵산추출부(100)로부터 핵산이 결합된 자성물질이 포함된 혼합액이 임시로 담기는 버퍼용기(210)와; 상기 버퍼용기(210)에 결합되어 자력에 의하여 핵산이 결합된 자성물질을 포집하는 자력작용부(220)를 포함할 수 있다.For example, the extraction buffer unit 200 includes a buffer container 210 in which a liquid mixture containing a magnetic material to which nucleic acids are bound from the nucleic acid extraction unit 100 is temporarily contained; It may include a magnetic force action unit 220 that is coupled to the buffer container 210 and collects the magnetic material to which the nucleic acid is bound by magnetic force.

상기 버퍼용기(210)는, 상기 핵산추출부(100)로부터 핵산이 결합된 자성물질이 포함된 혼합액이 임시로 담기는 구성으로서 다양한 구성이 가능하다.The buffer container 210 is configured to temporarily contain a liquid mixture containing a magnetic material to which nucleic acids are bound from the nucleic acid extraction unit 100, and various configurations are possible.

예로서, 상기 버퍼용기(210)는, 상측에 상기 핵산추출부(100)가 유입될 수 있도록 유입구가 형성되며, 측면에 후술하는 전달유로(450)가 결합될 수 있다.For example, the buffer container 210 has an inlet formed on the upper side so that the nucleic acid extracting unit 100 can flow in, and a delivery passage 450 to be described later may be coupled to the side.

여기서 상기 버퍼용기(210) 및 핵산추출부(100)는, 핵산이 결합된 자성물질이 포함된 혼합액의 유입을 선택적으로 수행할 수 있도록 제1밸브(410)가 설치된다.Here, the buffer container 210 and the nucleic acid extraction unit 100 are provided with a first valve 410 to selectively introduce the mixed solution containing the magnetic material to which the nucleic acid is bound.

상기 제1밸브(410)는, 상기 핵산추출부(100)에서 자성물질이 핵산을 결합되는 반응이 수행되는 동안, 예를 들면 미리 설정된 시간동안 닫힌 상태를 유지하며, 자성물질 결합 반응이 완료된 후 개방되어 핵산이 결합된 자성물질이 포함된 혼합액이 핵산추출부(100)로부터 버퍼용기(210)로 전달되도록 한다.The first valve 410 remains closed while a reaction in which a magnetic material is coupled to a nucleic acid is performed in the nucleic acid extracting unit 100, for example, for a predetermined time, and after the magnetic material binding reaction is completed. It is opened so that the mixed solution containing the magnetic material to which nucleic acids are bound is transferred from the nucleic acid extraction unit 100 to the buffer container 210.

한편, 후술하는 자력작용부(220)에 의하여 자성물질이 포집된 상태에서 나머지 혼합액 등이 제거될 필요가 있다.On the other hand, it is necessary to remove the remaining liquid mixture in a state in which the magnetic material is collected by the magnetic force applying unit 220 to be described later.

이에 상기 버퍼용기(210)는, 핵산이 결합된 자성물질을 제외한 나머지 물질을 배출하는 배출부(420)가 저면에 결합될 수 있다.Accordingly, the buffer container 210 may have a discharge portion 420 coupled to a bottom surface of the buffer container 210 to discharge the remaining materials except for the magnetic material to which the nucleic acid is bound.

이때 상기 배출부(420)는, 후술하는 자력작용부(220)에 의하여 자성물질이 충분히 포집될 수 있도록 제2밸브(421) 및 배관(422)로 구성될 수 있댜.At this time, the discharge part 420 may be composed of a second valve 421 and a pipe 422 so that the magnetic material can be sufficiently collected by the magnetic force applying part 220 to be described later.

상기 제2밸브(421)는, 후술하는 자력작용부(220)에 의하여 자성물질이 충분히 포집하는 동안, 예를 들면 미리 설정된 시간동안 닫힌 상태를 유지하며, 일정 시간 경과 후 개방되어 핵산이 결합된 자성물질을 제외한 나머지 물질을 배출한다.The second valve 421 remains closed for a predetermined time, for example, while the magnetic material is sufficiently collected by the magnetic force application unit 220 described later, and is opened after a certain period of time to allow nucleic acids to bind. Excluding the magnetic material, the rest of the material is discharged.

한편, 상기 배관(422)은, 하단에 배출물질이 담기는 배출용기(미도시)가 결합될 수 있다.On the other hand, the pipe 422 may be coupled to a discharge container (not shown) in which the discharge material is contained at the lower end.

상기 자력작용부(220)는, 상기 버퍼용기(210)에 결합되어 자력에 의하여 핵산이 결합된 자성물질을 포집하는 구성으로서, 다양한 구성이 가능하다.The magnetic force acting unit 220 is a component that is coupled to the buffer container 210 and collects magnetic materials to which nucleic acids are coupled by magnetic force, and various configurations are possible.

예로서, 상기 자력작용부(220)는, 복수의 영구자석으로 구성될 수 있다.For example, the magnetic force applying unit 220 may be composed of a plurality of permanent magnets.

이때, 상기 영구자석은, 자성물질을 포집하는 동안 상기 버퍼용기(210)의 외측에서 충분한 거리에 근접되고, 후술하는 전달유로(450)를 통하여 자성물질을 전달하는 경우 방해되지 않도록 상기 버퍼용기(210)로부터 이격되는 것이 바람직하다.At this time, the permanent magnet is close to a sufficient distance from the outside of the buffer container 210 while collecting the magnetic material, and the buffer container ( 210) is preferred.

이에 상기 영구자석은, 상기 버퍼용기(210)에 대하여 근접 또는 벌어지도록 이동가능하게 설치될 수 있다.Accordingly, the permanent magnet may be movably installed so as to approach or spread out with respect to the buffer container 210 .

다른 예로서, 상기 자력작용부(220)는, 전자석으로 구성될 수 있다.As another example, the magnetic force applying unit 220 may be composed of an electromagnet.

이때, 상기 전자석은, 자성물질을 포집하는 동안은 자력이 작용되도록 작동되며, 포집 후 전달유로(450)로 자성물질을 전달하는 경우 유동을 방해하지 않도록 자력이 제거될 수 있다.At this time, the electromagnet is operated so that magnetic force is applied while the magnetic material is collected, and when the magnetic material is transferred to the delivery passage 450 after collection, the magnetic force may be removed so as not to disturb the flow.

상기 전달유로(450)는, 일단에 상기 추출용 버퍼부(200)와 연결되어 증폭 시약이 담인 하나 이상의 증폭챔버(310)로 상기 추출용 버퍼부(200)로부터 핵산이 결합된 자성물질을 전달하는 구성으로서, 다양한 구성이 가능하다.The transmission passage 450, one end of which is connected to the extraction buffer part 200, transfers the nucleic acid-coupled magnetic material from the extraction buffer part 200 to one or more amplification chambers 310 containing amplification reagents. As a configuration, various configurations are possible.

예로서, 상기 전달유로(450)는, 일단이 상기 추출용 버퍼부(200)가 연결되고 길이방향으로 하나 이상의 증폭챔버(310)가 결합된 배관으로 구성될 수 있다.For example, the transmission passage 450 may be configured as a pipe to which one end is connected to the extraction buffer unit 200 and to which one or more amplification chambers 310 are coupled in the longitudinal direction.

한편, 상기 전달유로(450)는, 자성물질의 이동이 원활하도록 상기 버퍼용기(210)의 바닥보다 낮게 연결될 수 있다.Meanwhile, the transmission passage 450 may be connected lower than the bottom of the buffer container 210 so that the magnetic material moves smoothly.

그리고 상기 전달유로(450)는, 버퍼용기(210)와 결합되는 부분에서 제3밸브(440)가 설치될 수 있다.In addition, a third valve 440 may be installed in a portion of the transmission passage 450 coupled to the buffer container 210 .

상기 제3밸브(440)는, 상기 버퍼용기(210)가 상기 핵산추출부(100)로부터 혼합액을 전달받아 자성물질의 포집 후 나머지 혼합액 등을 배출할 때 닫힌 상태를 유지하며, 자성물질의 포집이 완료 또는 미리 설정된 시간이 경과된 후 개방되도록 작동된다.The third valve 440 maintains a closed state when the buffer container 210 receives the mixed solution from the nucleic acid extracting unit 100 and collects the magnetic material and discharges the remaining mixed liquid, etc., and collects the magnetic material. It is operated to open after this completion or a preset time has elapsed.

한편, 상기 버퍼용기(210)에 포집된 자성물질은 상기 전달유로(450)를 통하여 증폭챔버(310)로 전달을 촉진할 필요가 있다.On the other hand, the transfer of the magnetic material collected in the buffer container 210 to the amplification chamber 310 through the transfer passage 450 needs to be accelerated.

이에 상기 전달유로(450) 내 음압을 형성하여 상기 추출용 버퍼부(200)로부터 핵산이 결합된 자성물질의 이동을 유도하는 음압형성부(430)가 타단에 형성될 수 있다.Accordingly, a negative pressure forming unit 430 may be formed at the other end of the transmission passage 450 to induce movement of the magnetic material to which the nucleic acid is coupled from the extraction buffer unit 200 by forming a negative pressure.

상기 음압형성부(430)는, 상기 전달유로(450) 내 음압을 형성하여 상기 추출용 버퍼부(200)로부터 핵산이 결합된 자성물질의 이동을 유도하는 구성으로서, 진공펌프와 같은 공압제어장치와 연결되는 유로 상에 설치된 제3밸브로 구성될 수 있다.The negative pressure forming unit 430 forms a negative pressure in the transmission passage 450 to induce movement of the magnetic material to which the nucleic acid is coupled from the extraction buffer unit 200, and is a pneumatic control device such as a vacuum pump. It may be composed of a third valve installed on the flow path connected to.

상기 제3밸브는, 상기 버퍼용기(210)에 포집된 자성물질이 상기 전달유로(450)를 통하여 증폭챔버(310)로 전달될 때 개방되어 상기 전달유로(450)를 통한 충분한 유동이 발생되도록 한다.The third valve is opened when the magnetic material collected in the buffer container 210 is transferred to the amplification chamber 310 through the transmission passage 450 so that sufficient flow occurs through the transmission passage 450. do.

한편, 상기 전달유로(450)를 통한 공기 유동을 위하여, 상기 전달유로(450)의 일단에는 제4밸브(460)이 설치될 수 있다.Meanwhile, in order to flow air through the transmission passage 450, a fourth valve 460 may be installed at one end of the transmission passage 450.

상기 제4밸브(450)는, 상기 전달유로(450)를 통한 공기 유동을 위하여, 상기 전달유로(450)의 일단에 결합되어 상기 음압형성부(430) 작동시 함께 개방되어 상기 전달유로(450)를 통한 공기유동을 형성하게 된다.For air flow through the transmission passage 450, the fourth valve 450 is coupled to one end of the transmission passage 450 and is opened together when the negative pressure forming unit 430 operates. ) through which the air flow is formed.

한편, 상기 제4밸브(450)는, 상기 전달유로(450)의 일단에 설치되어 상기 전달유로(450)를 세정하기 위한 정제수의 공급을 함께 수행할 수 있다.Meanwhile, the fourth valve 450 is installed at one end of the delivery passage 450 to supply purified water for cleaning the delivery passage 450 together.

이상은 본 발명에 의해 구현될 수 있는 바람직한 실시예의 일부에 관하여 설명한 것에 불과하므로, 주지된 바와 같이 본 발명의 범위는 위의 실시예에 한정되어 해석되어서는 안 될 것이며, 위에서 설명된 본 발명의 기술적 사상과 그 근본을 함께하는 기술적 사상은 모두 본 발명의 범위에 포함된다고 할 것이다.Since the above has only been described with respect to some of the preferred embodiments that can be implemented by the present invention, as noted, the scope of the present invention should not be construed as being limited to the above embodiments, and the scope of the present invention described above It will be said that the technical idea and the technical idea together with the root are all included in the scope of the present invention.

100 : 핵산추출부 200 : 추출용 버퍼부
300 : 핵산증폭부 450 : 전달유로100: nucleic acid extraction unit 200: buffer unit for extraction
300: nucleic acid amplification unit 450: transfer channel

Claims (5)

추출된 검체로부터 자성물질에 결합시켜 핵산을 추출하는 핵산추출부(100)와;
상기 핵산추출부(100)로부터 핵산이 결합된 자성물질이 포함된 혼합액을 전달받아 핵산이 결합된 자성물질을 남기고 혼합액을 배출하는 추출용 버퍼부(200)와;
일단에 상기 추출용 버퍼부(200)와 연결되어 증폭 시약이 담인 하나 이상의 증폭챔버(310)로 상기 추출용 버퍼부(200)로부터 핵산이 결합된 자성물질을 전달하는 전달유로(450)를 포함하는 것을 특징으로 하는 핵산 추출 장치.A nucleic acid extraction unit 100 for extracting nucleic acids from the extracted specimen by binding to magnetic materials;
an extraction buffer unit 200 for receiving the mixed liquid containing the magnetic material to which the nucleic acid is coupled from the nucleic acid extracting unit 100 and discharging the mixed liquid while leaving the magnetic material to which the nucleic acid is bound;
At one end, a transfer passage 450 is connected to the extraction buffer unit 200 and transfers the magnetic material to which the nucleic acid is bound from the extraction buffer unit 200 to one or more amplification chambers 310 containing amplification reagents. Nucleic acid extraction device, characterized in that the. 청구항 1에 있어서,
상기 전달유로(450) 내 음압을 형성하여 상기 추출용 버퍼부(200)로부터 핵산이 결합된 자성물질의 이동을 유도하는 음압형성부(430)가 타단에 형성된 것을 특징으로 하는 핵산 추출 장치.The method of claim 1,
A nucleic acid extraction device, characterized in that a negative pressure forming unit 430 is formed at the other end to induce movement of the magnetic material to which the nucleic acid is bound from the extraction buffer unit 200 by forming a negative pressure in the transmission passage 450. 청구항 1에 있어서,
상기 추출용 버퍼부(200)는,
상기 핵산추출부(100)로부터 핵산이 결합된 자성물질이 포함된 혼합액이 임시로 담기는 버퍼용기(210)와;
상기 버퍼용기(210)에 결합되어 자력에 의하여 핵산이 결합된 자성물질을 포집하는 자력작용부(220)를 포함하는 것을 특징으로 하는 핵산 추출 장치.The method of claim 1,
The extraction buffer unit 200,
a buffer container 210 in which the mixed solution containing the magnetic material to which the nucleic acid is bound from the nucleic acid extraction unit 100 is temporarily contained;
Nucleic acid extracting device characterized in that it comprises a magnetic force action unit (220) coupled to the buffer container (210) to collect the magnetic material to which the nucleic acid is bound by magnetic force. 청구항 3에 있어서,
상기 버퍼용기(210)는, 핵산이 결합된 자성물질을 제외한 나머지 물질을 배출하는 배출부(420)가 저면에 결합되고,
상기 전달유로(450)는, 상기 버퍼용기(210)의 바닥보다 낮게 연결된 것을 특징으로 하는 핵산 추출 장치.The method of claim 3,
The buffer container 210 is coupled to the bottom surface of the discharge part 420 for discharging the remaining materials except for the magnetic material to which the nucleic acid is bound,
The delivery passage 450 is a nucleic acid extraction device, characterized in that connected lower than the bottom of the buffer container (210). 청구항 1 내지 청구항 4 중 어느 하나의 항에 있어서,
상기 전달유로(450)의 일단에는, 상기 전달유로(450)를 세정하기 위한 정제수의 공급을 위한 제4밸브(460)가 결합된 것을 특징으로 하는 핵산 추출 장치.According to any one of claims 1 to 4,
A nucleic acid extracting device, characterized in that a fourth valve 460 for supplying purified water for cleaning the delivery passage 450 is coupled to one end of the delivery passage 450.
KR1020210155407A 2021-11-12 2021-11-12 Device for extracting nucleic acids KR20230069404A (en)

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