KR20230058894A - Metarhizium pemphigi 111 strain for controlling bulb mite and uses thereof - Google Patents

Abstract

The present invention relates to a Metarhizium Pemphigi 111 strain (accession number: KACC 83052BP), which has mite control ability and UV-B resistance, a composition for controlling mites comprising the strain or a culture thereof as an active ingredient, and a mite control method comprising a step of treating the composition on plants, plant seeds, or plant planting soil.

Description

Metarhizium pemphigi 111 strain having root mite control ability and its use {Metarhizium pemphigi 111 strain for controlling bulb mite and uses its}

The present invention relates to a strain of Metarhizium pemphigi 111 having root mite control ability and uses thereof.

Various crop protection agents are used to control pests and plant diseases, which are pointed out as the biggest obstacles to plant growth, and research and development for the development of more effective protection agents are being actively conducted worldwide. Crop protection agents for pest control are almost indispensable for growing plants for economic purposes such as food production or for landscaping purposes, and the economic cost accordingly is also very high. Until now, almost all of these crop protection agents have been developed and used using chemicals, and thus, in recent years, crop protection agents based on these chemicals have been followed by various side effects such as harm to humans and animals, as well as destruction of the ecosystem. . In addition, in the case of insecticides using chemical substances, many pests develop resistance, making the control more difficult, and resistance of pests to these chemical pesticides is particularly severe in sucking micropests that occur many generations throughout the year. In order to solve the side effects of these crop protection agents, various eco-friendly control methods have been developed, and one of the methods that is attracting attention is the development and use of microbial insecticides using entomopathogenic microorganisms.

Microorganisms that can be used as materials for microbial insecticides include various microorganisms such as bacteria, viruses, and molds, and among them, insect pathogenic fungi have the longest history. Entomopathogenic fungi are microorganisms that cause disease and death to insects. In the process of invading and proliferating in insect hosts, entomopathogenic fungi produce various extracellular enzymes, toxins, and secondary metabolites to overcome the host insect's immune response or kill the host. It is also being used for competition with other microorganisms. Using these characteristics, research and development on antibacterial and antifungal activities by various metabolites of insect pathogenic fungi have also been actively conducted recently.

The entomopathogenic fungi most often used as microbial insecticide materials are fungi belonging to the genus Beauveria and Metarhizium . Insecticides against a wide variety of pests, such as lepidopteran pests and coleopteran pests, have been developed and marketed using these fungal strains. Since pesticides using these insect pathogenic fungi are developed using living fungi as materials, stability during storage, distribution, and treatment of the developed pesticides is evaluated as very important. Among various environmental factors that can affect the activity of fungi, heat stability and UV stability are considered to be very important factors. Therefore, in recent years, in the development of insecticides using fungi, studies to use these fungal strains with high heat stability and UV stability have been actively conducted.

Root mite ( Rhizoglyphus robini Claparede ) is a pest belonging to the Acarina family Acaridae and is widely distributed in the soil. do damage Root mite causes primary damage by physically attacking the roots in the soil, and then causes secondary damage such as decay and infection by pathogens as fungus or bacteria invade through wounds caused by the injury. In order to control root mites, synthetic pesticides such as bifenthrin, deltamethrin, promecarb, and diazinon are mainly used, but resistance to root mites is acquired and environmental pollution problems, etc. Because of this problem, it is considered a pest that is very difficult to control.

On the other hand, Korean Patent Publication No. 2016-0140560 discloses 'entomopathogenic fungus Metarhizium anisopleae SD4-2 strain and Beauveria bassiana SD15 strain having pest control and antibacterial activity', and Korean Patent Publication No. No. 2021-0012341 discloses 'Metarhizium anisopoliea JEF-214, Metarhizium anisopoliea JEF-279 or Metarhizium anisopoliea JEF-290 having a control effect on ticks, a composition for controlling ticks containing the same and a method for controlling ticks using the same', but this There is no description of the 'Metharia pempygi 111 strain having root mite control ability and its use' of the present invention.

The present invention has been derived from the above needs, and the present inventors show high insecticidal resistance to sucking pests such as Rhizoglyphus robini Claparede and control using insect pathogenic fungi, which is a biological control method with little resistance. In order to develop a method, as a result of selecting fungal strains having high killing power against root mite using 342 insect pathogenic fungal strains isolated and secured in Korea, Metarhizium pemphigi 111 strain is root mite The present invention was completed by confirming that it has high acaricidal activity against and excellent UV-B resistance.

In order to solve the above problems, the present invention provides a metarhizium pemphigi 111 strain (Accession Number: KACC 83052BP) having mite control ability and UV-B resistance.

In addition, the present invention provides a composition for controlling mites comprising the strain or its culture as an active ingredient.

In addition, the present invention provides a mite control method comprising the step of treating plants, plant seeds or plant planting soil with the composition.

Since the Metarhidium pempygi 111 strain of the present invention shows a specific insecticidal effect only against root mites, it can be used as a very safe pest control source for humans and animals and the environmental ecosystem.

1 is a photograph showing dead root mite caterpillars infected with insect pathogenic fungal strains.
Figure 2 is a graph showing the killing power for 7 days for 11 insect pathogenic fungal strains ( Rhizoglyphus robini Claparede). The control was treated only with Tween-80, and the vertical line represents the standard error. 111, Metarhizium pemphigi 111; 1101, M. pemphigi 1101; 312, M. pingshaense 312; 441, M. pingshaense 441; 322, M. anisopliae 322; 432, M. anisopliae 432; 481, M. anisopliae 481; 4141, M. anisopliae 4141; 4183, M. anisopliae 4183; 423, M. anisopliae 423; 4312, M. anisopliae 4312.

In order to achieve the object of the present invention, the present invention provides a metarhizium pemphigi 111 strain (Accession Number: KACC 83052BP) having mite control ability and UV-B resistance.

The Metarhizium fempigi strain of the present invention is an entomopathogenic fungus, and the entomopathogenic fungus causes fungal diseases in invertebrates, including insects, to kill the host, thereby controlling the density of the host in a natural state, and depending on the type of fungus Since insects that can cause disease are limited, they only damage target pests and are not toxic to the environment or other humans, and it is reported that resistance mechanisms by insects are rarely expressed, making it possible to control pests that are difficult to control. A lot of research and development is being done for this purpose.

The present inventors isolated fungi with high killing power against Rhizoglyphus robini Claparede using 342 insect pathogenic fungal strains, and as a result, 11 strains belonging to the genus Metarhizium spp. And it was confirmed that it had excellent UV-B resistance, among which, the Metarhizium pempygi 111 strain was deposited with the Agricultural Biotechnology Research Institute (KACC) on August 20, 2021, and was given the accession number KACC 83052BP.

The Metarhizium pempygi 111 strain of the present invention was identified as a Metarhizium pempygi strain through the identification of the ITS region sequence composed of the nucleotide sequence of SEQ ID NO: 1 and the EF-1α region sequence composed of the nucleotide sequence of SEQ ID NO: 2 .

The present invention also provides a composition for controlling mites comprising the strain or its culture as an active ingredient.

The mite control composition of the present invention contains, as an active ingredient, Metarhizium pemphigi 111 strain (Accession Number: KACC 83052BP) or a culture thereof having high acaricidal activity against Rhizoglyphus robini Claparede, It has an anti-mite effect.

In the composition of the present invention, the 'culture' may include a culture of Metarhizium pempygi 111 strain, a concentrate of the culture, or a dried product of the culture, but is not limited thereto.

In addition, the culture may be used in the form of the culture itself containing the strain of the present invention, or may be subjected to centrifugation or filtration to remove the culture medium in the culture medium and recover only the concentrated cells, such Steps can be performed as needed by those skilled in the art. Concentrated cells can be frozen or lyophilized according to conventional methods to preserve their activity.

The culture can be obtained by culturing in large quantities by a conventional microbial culture method, and a medium containing a carbon source, a nitrogen source, vitamins and minerals can be used as the culture medium, and the carbon source includes starch, sucrose, Citrate, maltose, glucose, mannitol, glycerol or xylose may be used, but is not limited thereto.

The method of culturing the strain of the present invention may be cultured according to a method commonly used in the art, and is not limited to a particular method.

When using the Metarhizium pempygi 111 strain or its culture obtained in the step of culturing the strain of the present invention as an additive, the strain or the culture of the strain may be added as it is or used together with other additives. It can be used appropriately depending on the method. The mixing amount of the components can be suitably determined depending on the intended use thereof.

The mite control composition according to the present invention may be prepared, for example, in the form of directly sprayable solutions, powders and suspensions or as highly concentrated aqueous, oily or other suspensions, dispersions, emulsions, oily dispersions, pastes, dusts, scattering materials or granules. It may, but is not limited thereto. In addition, the composition for controlling plant diseases may be used by spraying, spraying, spraying, scattering or pouring. The form of use depends on the intended purpose; in all cases, it should be such that the distribution of the composition according to the invention is as fine and uniform as possible.

In addition, the composition for controlling mites of the present invention can be formulated in various forms. The formulation can be prepared, for example, by adding a solvent and/or a carrier. Often, inactive additives and surface-active substances such as emulsifiers or dispersants are mixed into the formulation. Suitable surface-active substances are aromatic sulfonic acids (eg lignosulfonic acids, phenol-sulfonic acids, naphthalene- and dibutylnaphthalenesulfonic acids), fatty acids, alkyl- and alkylarylsulfonates, alkyl lauryl ethers, alkalis of fatty alcohol sulfates. Metals, alkaline earth metals, ammonium salts, sulfated hexa-, hepta- and octadecanols, salts of fatty alcohol glycol ethers, sulfonates naphthalene and its derivatives, condensates of formaldehyde, naphthalene or naphthalenesulfonic acids, phenols and formaldehyde Condensates, polyoxyethyleneoctyl phenol ethers, ethoxylated isooctyl-, octyl- or nonylphenols, alkylphenyl or tributylphenyl polyglycol ethers, alkylarylpolyether alcohols, isotridecyl alcohols, fatty alcohol/ethylene oxide condensates , ethoxylated castor oil, polyoxyethylene alkyl ether or polyoxypropylene, lauryl alcohol polyglycol ether acetate, sorbitol ester, lignin-sulfite waste liquid or methyl cellulose, but is not limited thereto.

Suitable solid carrier materials are, in principle, all porous, agriculturally acceptable carriers, for example mineral earths (such as silica, silica gel, silicates, talc, kaolin, limestone, lime, chalk, boulders, loess, clays, Dolomite, diatomaceous earth, calcium sulfate, magnesium sulfate, magnesium oxide, ground synthetics), fertilizers (e.g. ammonium sulfate, ammonium phosphate, ammonium nitrate, urea), vegetable products (e.g. grain flour, bark meal, wood meal) and nut shell powder) or cellulose powder, but is not limited thereto. In addition, one type or a mixture of two or more types may be used for the above solid carrier.

The mite control composition of the present invention may be used alone as an active ingredient, or in combination with two or more other acaricide substances.

In addition, the method for preparing the mite control composition may use any method known in the art, and is not particularly limited to a specific method.

The composition for controlling mites of the present invention can be mixed with a spreading agent, a penetrating agent, or a surfactant in order to enhance plant absorption and effectiveness.

The present invention also provides a mite control method comprising the step of treating plants, plant seeds or plant planting soil with the composition.

In the mite control method according to the present invention, the composition has a high acaricidal activity on Rhizoglyphus robini Claparede Metarhizium pemphigi 111 strain (Accession Number: KACC 83052BP) or its culture is effective contains as an ingredient

The mite control method of the present invention may be performed by immersing or drenching, ie, spraying, an effective amount of a composition containing the Metarhidium pempygi 111 strain or its culture as an active ingredient. In the case of the immersion method, an effective amount of the composition may be poured into the soil around the plant or the seed may be immersed in the effective amount of the composition.

The 'effective amount' of the present invention is an amount sufficient to produce beneficial or desired results, uniformly diluting the Metarhizium femphigi 111 strain or its culture with water to control mites, especially root mites ( R. robini Claparede) After that, it can be sprayed on plants and farmland using an appropriate spraying device such as a power sprayer.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only to illustrate the present invention, and the content of the present invention is not limited to the following examples.

Materials and Methods

spotted mite

Root mite ( R. robini ) was used in experiments while supplying onions and breeding under laboratory conditions at a temperature of 25 ° C, a relative humidity of 80-90%, and a light condition of 12L: 12D.

Entomopathogenic fungal strains

1) Cultivation of fungal strains

342 fungal strains (Shin et al., Biocontrol Science and Technology 2013, 23(3):288-304) isolated from national soils in Korea were used (Table 1).

342 strains of entomopathogenic fungi isolated in Korea mold type number of strains mold type number of strains Acremonium spp. One Metarhizium lepidiotum One Aspergillus spp. 8 Metarhizium pemphigi 16 Beauveria bassiana 110 Mucoromycotina spp. One Beauveria brongniartii 8 Myrothecium spp. 5 Beauveria pseudobassiana 8 Paecilomyces carneus 3 Bionecria spp. 7 Paecilomyces marquandii 5 Chaunopycnis spp. 23 Paraconiothyrium spp. 2 Clonostachys rosea One Phialocephala spp. One Fusarium spp. 2 Pochonia spp. 17 Isaria farinosa 12 Purpureocilium lilacinum 4 Isaria fumosorosea 6 Simplicillium spp. One Isaria spp. 22 Tolypocladium spp. 3 Lecanicillium spp. 8 Verticillium spp. 3 Metarhizium anisopliae 64

The fungal strain was cultured on a potato dextrose agar (PDA) plate at 25°C for 14 days, then the 14-day-old fungal conidia were scraped off the PDA plate and the material was placed in a 0.05% Tween-80 (Difco, USA) solution. Collected by resuspension. The conidial suspension was vigorously stirred and filtered through cotton gauze to remove mycelial debris. The concentration of filtered spores was measured using a hemocytometer.

2) Identification of fungal strains

Identification of the strains confirmed to be pathogenic to root mite was performed by molecular biological identification along with morphological identification through spore shape and color through microscopic observation. For molecular biological identification, genomic DNA extraction of fungi was carried out by using fungal DNA extraction buffer (0.2 M Tris-Cl (pH 7.5), 0.5 M Nacl, 10 nM EDTA (pH 8.0) and 1% ( w/v) SDS) and centrifuged with phenol-chloroform-isoamyl alcohol (25:24:1) to purify DNA. The purified DNA solution was precipitated using cold ethanol, centrifuged, and dissolved in sterile distilled water for use in the experiment.

PCR primers for molecular biological identification of the isolated strain are ITS1 (5'-TCCGTAGGTGAACCTGCGG-3', SEQ ID NO: 3) and ITS4 (5'-TCCTCCGCTTATTGATATGC-3) for amplification of the internal transcribed spacer (ITS1-5.8S-ITS2) part. ', SEQ ID NO: 4) primers were used, and for amplification of the elongation factor-1 aplha part, 1577F (5'-CARGAYGTBTACAAGATYGGTGG-3', SEQ ID NO: 5) and 2218R (5'-ATGACACCRACRGCRACRGTYTG-3', SEQ ID NO: 6) Primer was used. The PCR reaction was performed using AccuPower PCR PreMix (Bioneer Co., Korea) under conditions of denaturation at 94°C for 5 minutes, annealing at 55°C for 30 seconds, and extension at 72°C for 1 minute 35 times in a Thermal Cycler. (TaKaRa, Japan).

After the PCR reaction, the amplification product was analyzed by electrophoresis using a 1.0% agarose gel and purified using a Power Gel Extraction Kit (Dyne Bio Inc., Korea). The purified PCR products were sequenced by requesting direct sequencing to Macrogen (Korea). The base sequence was aligned using Multalin (http://multalin.toulouse.inra.fr/multalin/) and then compared and analyzed with other previously reported fungal sequences using the BLAST search tool.

Bioassay

1) Isolation of root mite pathogenic fungi

In order to isolate fungi pathogenic to root mite, 14-day-old fungal spores were used for bioassay. Spore suspensions with viability greater than 90% were used for bioassays. Spore viability was determined on SDAY+B medium (Saboraud dextrose agar, 1% Yeast extract, 0.05% benomyl, pH 5.6).

342 fungal strains were randomly divided into 4 groups, and spore suspensions of each group of fungal strains were combined into one and used for bioassay. In the bioassay, 50 adult root mites were inoculated with the fungal spore suspension of each group by immersion, and the inoculated root mites were observed every day for 10 days, and each larvae were collected. Root mite larvae were recognized as death caused by fungi only when the occurrence of mold on the epidermis was visually confirmed.

In order to isolate pathogenic fungi from dead root mite caterpillars, fungal spores were collected from caterpillars with a sterilized toothpick, suspended in 0.02% Tween-80 solution, and then SDA-D50 (sabouraud dextrose agar, 100 μg /ml chloramphenicol, 50 μg/ml streptomycin, 50 μg/ml dodine). Thereafter, colonies formed while culturing at 25 ° C. in the dark for 7 days were isolated, and again inoculated into PDA medium to isolate pure fungi, complete morphological and molecular biological identification, and then used in the next experiment.

2) Acaricidal activity of fungi

Fungal strains confirmed to be pathogenic for root mite were cultured in PDA (potato dextrose agar) medium for 2 weeks to induce sporulation, and then the resulting spores were tested using 0.05% Tween-80 for quantitative pathogenicity assay. A spore suspension was prepared, counted using a hemocytometer, and used in the experiment. Before the bioassay, all spores with a survival rate of 90% or more were used. The spore concentration of each fungal strain was 1 x 10 ⁷ spores/ml and 30 root mites were immersed and inoculated. Larvae were observed and collected every day for 7 days after inoculation, and death caused by fungi was recognized only when fungi were visually confirmed on the surface of the larvae. As a control, only 0.05% Tween-80 solution was treated with root mites.

Analysis of UV-B resistance of fungal spores

In order to evaluate the UV-B resistance of spores of fungal strains, 20 µl (5 x 10 ⁶ conidia/ml) of spore suspensions were plated on SDA-B medium without spreading. Spores (conidia) were exposed to irradiance of 0.1 or 0.2 J cm ⁻² at 25° C. in a UV irradiation chamber (Bio-Link-BLX-E254, France), and cultured at 25° C. The control was cultured under the same conditions in the same medium without UV-B irradiation. The germination rate was calculated by comparing the germination level of the treated group compared to the untreated control group. A minimum of 100 spores were used for each round of each experiment, performed in triplicate.

statistical analysis

The evaluation of acaricidal activity and thermal stability was repeated three times, and the result value was tested for significance at a significance level of 0.05 or less using the SPSS statistical program. Data are expressed as mean ± standard error (SE).

Example 1. Isolation of the spotted mite pathogenic fungus

1) Isolation of pathogenic fungi

342 strains of entomopathogenic fungi were grouped into 4 groups, and spore suspensions were prepared for each group to test pathogenicity against root mites. As a result, among 200 root mites, 38 mites were killed by fungi.

As a result of isolating fungi from root mite caterpillars whose death by fungi was confirmed, 57 strains were isolated. For the isolated strains, molecular biological identification of the ITS region and Elongation factor-1 aplha region was performed along with morphological identification based on spore shape and color. As a result, Beauveria bassiana ( Beauveria bassiana ) 29 strains, Metarhizium anisopliae ( Metarhizium anisopliae ) 19 strains, Metarhizium pemphigi ( M. pemphigi ) 5 strains, Metarhizium pingchaens ( M. pingshaense ) Two strains, Akanthomyces attenuatus ( Akanthomyces attenuatus ) One strain and Akanthomyces besicolor ( A. versicolor ) One strain was identified (see Tables 2 to 3).

As a result of re-examination of pathogenicity for Rhizoglyphus robini using 57 identified strains, pathogenicity was reconfirmed only in 15 strains, among which strains with 100% matching molecular biological identification results were judged as the same strain Therefore, only one strain was used in the next experiment. Among the finally isolated strains, Metatridium anisopliae ( M. anisopliae ) 7 strains, Metatridium pemphigi ( M. pemphigi ) 2 strains, Metatridium pingsharens ( M. pingshaense ) 2 strains was selected.

Example 2. Killing power against spotted mites

The viability of 11 strains whose pathogenicity was confirmed for root mite was compared and evaluated (FIG. 1). As a result, the killing power of the 11 strains against root mites varied depending on the strain, and ranged from 38% to 100% at 7 days after treatment. Among them, Metarhizium anisopleae 432 and 4312 strains, and Metarhizium pemphigi 111 strains showed 100% killing power. Among them, Metarhizium anisoplea 432 and Metarhizium pempygi 111 strains showed high killing power of 99% or more even 5 days after treatment.

Example 3. UV-B resistance of fungal spores

UV-B resistance was compared and evaluated for spores of three strains (Meterydium anisoplea 432 and 4312, Metarhizium pemphigi 111) with 100% killing power on day 7 against root mites. As a result, as shown in Table 4 below, in the condition of UV-B 0.1 J cm ^-2 treatment, the Metarhidium pempygi 111 strain showed a spore germination rate of 9.0 ± 2.0%, compared to the UV-B strains of Metarhizium anisopleae. It was found that tolerance was high.

Comparison of UV-B resistance Strain Spore germination rate (%) UV-B exposure conditions 0.1 J cm ^-2 0.2 J cm ^-2 M. pemphigi 111 9.0±2.0 1.6±0.5 M anisopliae 432 6.8±0.8 1.3±0.6 M anisopliae 4312 3.0±1.0 0.0±0

Summarizing the above results, it was confirmed that the M. pemphigi 111 strain is a useful strain for root mite control because it has high killing power and UV-B resistance to root mites. The Metarhizium Pempigi 111 strain was deposited with the Korean Agricultural Culture Collection (KACC) on August 20, 2021, and was given the accession number KACC 83052BP from the depository institution.

Agricultural Biotechnology Research Institute KACC83052BP 20210820

<110> Chungbuk National University Industry-Academic Cooperation Foundation <120> Metarhizium pemphigi 111 strain for controlling bulb mite and uses it <130> PN21383 <160> 6 <170> KoPatentIn 3.0 <210> 1 <211> 541 <212> DNA <213> unknown <220> 223 <223> <400> 1 agggatcatt accgagttta caactcccaa acccaatgtg aactatacct gtctaccgtt 60 gcctcggcgg gttcgcccgc cgagggaccg acaaataaac tcttgtattt ctatctttag 120 catgtctgag tggaatcata aacaaatgaa tcaaaacttt caacaacgga tctcttggtt 180 ctggcatcga tgaagaacgc agcgaaatgc gataagtaat gtgaattgca gaattcaggtg 240 aatcatcgaa tctttgaacg cacattgcgc ccgccagtat tctggcgggc atgcctgttc 300 gagcgtcatt acaaccctca agccccccgg cttggtgttg gggaccggcc accggtgccc 360 tgctgcttcc gcggcaggcg cacccggccg cccccgaaat gaattggcgg ccccgtcgcg 420 gcctccctct gcgtagtagc acacatctcg cagctggagc gcggcgcggc cactgccgta 480 aaacgcacca acttttttta cagttgacct cgaatcaggt aggaataccc gctgaactta 540 a 541 <210> 2 <211> 517 <212> DNA <213> unknown <220> 223 <223> <400> 2 tattggaact gtccctgtcg gccgtatcga gactggtgtc ctcaagcccg gtatggtcgt 60 taccttcgct ccctccaacg tcaccactga agtcaagtcc gtggagatgc accacgagca 120 gctttctgag ggtgtccccg gtgacaacgt tggtttcaac gtgaagaacg tttctgtcaa 180 ggaaatccgc cgtggtaacg ttgctggtga ctccaagaac gaccccccca tgggcgccgc 240 ttctttcgat gcccaggtca tcgttctcaa ccaccccggc caggtcggtg ctggttacgc 300 tcccgtcctc gattgtcaca ccgcccacat tgcctgcaag ttctccgaga tcaaggagaa 360 gattgaccga cgtaccggta aggctgttga gtctgccccc aagttcatca agtccggtga 420 ctctgccatc gtcaagatgg ttccctccaa gcccatgtgc gttgaggctt tcaccgatta 480 ccctcccctg ggccgtttcg ccgtccgtga tatgcgt 517 <210> 3 <211> 19 <212> DNA <213> artificial sequence <220> <223> primer <400> 3 tccgtaggtg aacctgcgg 19 <210> 4 <211> 20 <212> DNA <213> artificial sequence <220> <223> primer <400> 4 tcctccgctt attgatatgc 20 <210> 5 <211> 23 <212> DNA <213> artificial sequence <220> <223> primer <400> 5 cargaygtbt acaagatygg tgg 23 <210> 6 <211> 23 <212> DNA <213> artificial sequence <220> <223> primer <400> 6 atgacaccra crgcracrgt ytg 23

Claims (5)

Metarhizium pemphigi 111 strain (accession number: KACC 83052BP) having mite control and UV-B resistance. The method of claim 1, wherein the mite is a root mite ( Rhizoglyphus robini Claparede), characterized in that Metarhizium pempygi 111 strain. A composition for controlling mites comprising the strain or culture thereof of claim 1 as an active ingredient. A method for controlling mites, comprising the step of treating plants, plant seeds or plant planting soil with the composition of claim 3. The method of claim 4, wherein the plant is garlic, green onion, chives, onion, leek, potato, sweet potato, lily, tulip, gladiolus, daffodil, caladium, iris, hyacinth, crocus, dahlia or tamarisk, characterized in that, How to get rid of mites.

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