KR20230023598A - A preparation method of sourdough and rice bread using rice fermentation starter with increased storage property - Google Patents
A preparation method of sourdough and rice bread using rice fermentation starter with increased storage property Download PDFInfo
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Images
Classifications
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- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D8/00—Methods for preparing or baking dough
- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D13/00—Finished or partly finished bakery products
- A21D13/04—Products made from materials other than rye or wheat flour
- A21D13/047—Products made from materials other than rye or wheat flour from cereals other than rye or wheat, e.g. rice
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D13/00—Finished or partly finished bakery products
- A21D13/06—Products with modified nutritive value, e.g. with modified starch content
- A21D13/064—Products with modified nutritive value, e.g. with modified starch content with modified protein content
- A21D13/066—Gluten-free products
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D15/00—Preserving finished, partly finished or par-baked bakery products; Improving
- A21D15/02—Preserving finished, partly finished or par-baked bakery products; Improving by cooling, e.g. refrigeration, freezing
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D8/00—Methods for preparing or baking dough
- A21D8/06—Baking processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
Abstract
Description
본 발명은 쌀 발효 스타터를 이용한 쌀빵 및 이의 제조방법에 관한 것으로, 구체적으로 a) 쌀을 포함하는 현탁액을 락토바실러스 플란타룸 (Lactobacillus plantarum) 균주로 발효하여 쌀 발효 스타터를 제조하는 단계; b) 상기 쌀 발효 스타터를 포함하는 사워도우(Sourdough)를 제조하는 단계; c) 상기 사워도우를 발효하는 단계; 및 d) 상기 발효된 사워도우를 굽는 단계를 포함하는 쌀빵의 제조방법; 상기 방법에 의해 제조된 쌀빵; a) 쌀을 포함하는 현탁액을 락토바실러스 플란타룸 (Lactobacillus plantarum) 균주로 발효하여 쌀 발효 스타터를 제조하는 단계; 및 b) 상기 쌀 발효 스타터를 포함하는 단계를 포함하는 사워도우(Sourdough)의 제조방법; 및 상기 사워도우를 포함하는 가공식품에 관한 것이다.The present invention relates to rice bread using a rice fermentation starter and a manufacturing method thereof, and specifically, a) preparing a rice fermentation starter by fermenting a suspension containing rice with a Lactobacillus plantarum strain; b) preparing sourdough containing the rice fermentation starter; c) fermenting the sourdough; and d) a method for producing rice bread comprising the step of baking the fermented sourdough; Rice bread prepared by the above method; a) preparing a rice fermentation starter by fermenting a suspension containing rice with a strain of Lactobacillus plantarum ; And b) a method for producing sourdough comprising the step of including the rice fermentation starter; And it relates to processed food containing the sourdough.
쌀의 영양적 가치를 살펴보면, 쌀의 전분은 뇌의 활동을 돕고 비만과 당뇨병을 예방하는데 효과적이며, 비록 단백질 함량과 필수 아미노산 함량은 낮지만 그 질만큼은 모든 곡류 중에서 가장 우수한 단백질 자원을 갖고 있어 콜레스테롤 저하와 혈압조절, 암예방 등에 효과가 있는 것으로 알려져 있다. Looking at the nutritional value of rice, the starch of rice is effective in helping brain activity and preventing obesity and diabetes. It is known to be effective in lowering blood pressure, controlling blood pressure, and preventing cancer.
쌀을 주식으로 하는 우리나라에서는 쌀 가공식품인 떡을 오랜 기간동안 애용했으나, 1990년대 이후 결제가 성장하면서 먹거리의 다양화, 식생활의 서구화와 함께 빵이 그 자리를 대체하면서 쌀의 소비가 감소하기 시작했다. 급격한 쌀 소비의 감소로 농업전체의 구조개선과 소득보전을 위한 대책 마련이 필요하게 되었으며, 쌀 소비량의 감소로 인하여 늘어난 재고처리 문제도 시급한 과제로 다가 왔다. 이를 위하여 새로운 쌀 수요창출이 요구되고 있으며, 이를 위하여 쌀 가공식품의 개발도 필요한 실정이며, 쌀을 이용하여 빵을 제조하는 시도들이 많이 이루어졌다.In Korea, where rice is the staple food, rice cakes have been popular for a long time, but since the 1990s, as payments have grown, bread has replaced them along with the diversification of food and westernization of dietary life, and rice consumption has begun to decrease. did. Due to the rapid decrease in rice consumption, it was necessary to prepare measures for structural improvement and income preservation of the entire agricultural sector. To this end, new demands for rice are being created, and for this purpose, the development of processed rice foods is also required, and many attempts have been made to manufacture bread using rice.
스타터(starter)란 발효유, 치즈, 발효버터 등의 발효유제품의 제조용으로 사용되는 미생물의 배양물을 의미하며, 여기에는 유산균 스타터, 효모 스타터, 유산균을 2종 이상 혼합한 혼합 스타터 등이 있다. 이와 같은 스타터를 이용한 자연발효법은 제빵법의 기법으로서 사용되고 있으며, 상기 스타터로 사용되는 유산균으로는 락토바실러스 속(Lactobacillus 속), 락토코쿠스 속(Lactococcus 속), 스트렙토코쿠스 속(Streptococcus 속), 비피도박테리움 속(Bifidobacterium 속) 균들이 있다.A starter refers to a culture of microorganisms used for the manufacture of fermented milk products such as fermented milk, cheese, and fermented butter, and includes a lactic acid bacteria starter, a yeast starter, and a mixed starter in which two or more types of lactic acid bacteria are mixed. The natural fermentation method using such a starter is used as a baking technique, and the lactic acid bacteria used as the starter include Lactobacillus genus, Lactococcus genus, Streptococcus genus, There are bacteria of the genus Bifidobacterium.
사워도우는 발효빵의 제조에 사용되어, 밀단백질인 글루텐(gluten)에서 발효를 통하여 생성된 이산화탄소가 글루텐 네트워크에 포집된 채 구워져 빵 고유의 특성을 형성한다. 그러나 쌀에는 글루텐이 없기 때문에 밀가루 빵의 구조를 갖기 어려워 빵 고유의 맛과 질감을 살리기 어려운 점이 있다. 그러나, 최근 글루텐 불내성 환자나 위장 장해를 일으키는 셀리악병(Celiac Disease) 환자가 증가하고 있어서 글루텐-프리 식품에 대한 관심과 수요가 늘고 있다. 또한, 쌀은 밀가루에 비해 영양학적 가치가 높으므로, 쌀빵 개발에 대한 필요성이 높아지고 있으며, 쌀 소비촉진의 측면에 있어서도 쌀빵 개발이 필요한 실정이다.Sourdough is used in the manufacture of fermented bread, and carbon dioxide generated through fermentation in gluten, a wheat protein, is baked while being captured in the gluten network to form unique characteristics of bread. However, since rice does not contain gluten, it is difficult to have the structure of wheat bread, so it is difficult to preserve the unique taste and texture of bread. However, interest and demand for gluten-free foods are increasing as the number of patients with gluten intolerance or celiac disease causing gastrointestinal disorders has recently increased. In addition, since rice has a higher nutritional value than wheat flour, the need for rice bread development is increasing, and rice bread development is also needed in terms of promoting rice consumption.
이러한 배경하에, 본 발명자들은 주재료로서 쌀을 포함하는 저장성이 증가된 쌀빵을 제조하고자 예의 노력한 결과, 락토바실러스 플란타룸 (Lactobacillus plantarum) 균주를 포함하는 쌀 발효 스타터가 첨가된 쌀빵을 제조하고, 상기 쌀빵의 쫄깃함 및 저장성이 현저히 우수한 것을 확인함으로써, 본 발명을 완성하였다.Under this background, the present inventors have made diligent efforts to manufacture rice bread with increased shelf life containing rice as a main material, and as a result, Lactobacillus plantarum ( Lactobacillus plantarum ) To prepare rice bread to which a rice fermentation starter containing a strain is added, the above The present invention was completed by confirming that the chewiness and storability of rice bread were remarkably excellent.
본 발명의 하나의 목적은 a) 쌀을 포함하는 현탁액을 락토바실러스 플란타룸 (Lactobacillus plantarum) 균주로 발효하여 쌀 발효 스타터를 제조하는 단계; b) 상기 쌀 발효 스타터를 포함하는 사워도우(Sourdough)를 제조하는 단계; c) 상기 사워도우를 발효하는 단계; 및 d) 상기 발효된 사워도우를 굽는 단계를 포함하는 쌀빵의 제조방법을 제공하는 것이다.One object of the present invention is a) preparing a rice fermentation starter by fermenting a suspension containing rice with a Lactobacillus plantarum strain; b) preparing sourdough containing the rice fermentation starter; c) fermenting the sourdough; And d) to provide a method for producing rice bread comprising the step of baking the fermented sourdough.
본 발명의 다른 하나의 목적은 상기 방법에 의해 제조된 쌀빵을 제공하는 것이다.Another object of the present invention is to provide rice bread prepared by the above method.
본 발명의 또 다른 하나의 목적은 a) 쌀을 포함하는 현탁액을 락토바실러스 플란타룸 (Lactobacillus plantarum) 균주로 발효하여 쌀 발효 스타터를 제조하는 단계; 및 b) 상기 쌀 발효 스타터를 포함하는 단계를 포함하는 사워도우(Sourdough)의 제조방법을 제공하는 것이다.Another object of the present invention is a) preparing a rice fermentation starter by fermenting a suspension containing rice with Lactobacillus plantarum strain; And b) to provide a method for producing sourdough comprising the step of including the rice fermentation starter.
본 발명의 또 다른 하나의 목적은 상기 사워도우를 포함하는 가공식품을 제공하는 것이다Another object of the present invention is to provide a processed food containing the sourdough.
이를 구체적으로 설명하면 다음과 같다. 한편, 본 발명에서 개시된 각각의 설명 및 실시형태는 각각의 다른 설명 및 실시 형태에도 적용될 수 있다. 즉, 본 발명에서 개시된 다양한 요소들의 모든 조합이 본 발명의 범주에 속한다. 또한, 하기 기술된 구체적인 서술에 의하여 본 발명의 범주가 제한된다고 볼 수 없다.A detailed description of this is as follows. Meanwhile, each description and embodiment disclosed in the present invention may also be applied to each other description and embodiment. That is, all combinations of the various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be seen that the scope of the present invention is limited by the specific descriptions described below.
상기 목적을 달성하기 위한 본 발명의 하나의 양태는, a) 쌀을 포함하는 현탁액을 락토바실러스 플란타룸 (Lactobacillus plantarum) 균주로 발효하여 쌀 발효 스타터를 제조하는 단계; b) 상기 쌀 발효 스타터를 포함하는 사워도우(Sourdough)를 제조하는 단계; c) 상기 사워도우를 발효하는 단계; 및 d) 상기 발효된 사워도우를 굽는 단계를 포함하는 쌀빵의 제조방법을 제공한다.One aspect of the present invention for achieving the above object is, a) preparing a rice fermentation starter by fermenting a suspension containing rice with a Lactobacillus plantarum strain; b) preparing sourdough containing the rice fermentation starter; c) fermenting the sourdough; And d) providing a method for producing rice bread comprising the step of baking the fermented sourdough.
본 발명에 있어서, 상기 락토바실러스 플란타럼의 일 예로는, 본 발명자의 선행특허인 한국 등록특허공보 제10-1634270호에 기재된 락토바실러스 플란타럼 JSA22, 즉 기탁번호 KACC91973P를 갖는 락토바실러스 플란타럼일 수 있다. 상기 균주는 전통 장류에서 분리 및 선발된 유산균으로서, 항균활성, 인공소화액 생존율 및 부착성이 우수한 것이 특징이다.In the present invention, an example of the Lactobacillus plantarum is Lactobacillus plantarum JSA22 described in Korean Patent Registration No. 10-1634270, which is a prior patent of the present inventor, that is, Lactobacillus plantarum having accession number KACC91973P It could be rum. The strain is a lactic acid bacterium isolated and selected from traditional fermented soybeans, and is characterized by excellent antibacterial activity, artificial digestive juice survival rate and adhesion.
본 발명의 용어, "스타터(starter)"는 발효유, 치즈, 발효버터 등의 발효유제품의 제조용으로 사용되는 미생물의 배양물을 의미하며, 여기에는 유산균 스타터, 효모 스타터, 유산균을 2종 이상 혼합한 혼합 스타터 등이 포함될 수 있다. 본 발명에 있어서, 상기 스타터로 사용되는 유산균으로는 락토바실러스 속(Lactobacillus 속), 락토코쿠스 속(Lactococcus 속), 스트렙토코쿠스 속(Streptococcus 속), 비피도박테리움 속(Bifidobacterium 속) 균으로 이루어진 군으로부터 선택된 1종 이상일 수 있으며, 구체적으로, 락토바실러스 플란타룸 (Lactobacillus plantarum)을 의미할 수 있으며, 보다 구체적으로, 락토바실러스 플란타룸 (Lactobacillus plantarum) JSA22를 의미할 수 있다. 또한, 상기 용어 "쌀 발효 스타터"는 쌀을 포함하는 현탁액에 유산균을 접종하여 발효시킨 스타터를 의미한 것일 수 있다. 상기 쌀은 쌀가루 및 쌀배아를 포함하는 것을 의미할 수 있다. 상기 현탁액은 당류를 추가로 포함할 수 있으며, 구체적으로 수크로스(sucrose)를 포함할 수 있으나, 이에 제한되는 것은 아니다. 상기 쌀 발효 스타터에 포함되는 쌀가루는 글루텐이 포함되어 있지 않는 것을 특징으로 한다.As used herein, the term "starter" refers to a culture of microorganisms used for the manufacture of fermented milk products such as fermented milk, cheese, and fermented butter, in which two or more lactic acid bacteria starters, yeast starters, and lactic acid bacteria are mixed Mixed starters and the like may be included. In the present invention, the lactic acid bacteria used as the starter include Lactobacillus, Lactococcus, Streptococcus, and Bifidobacterium. It may be one or more species selected from the group consisting of, specifically, Lactobacillus plantarum ( Lactobacillus plantarum ) It may mean, more specifically, Lactobacillus plantarum ( Lactobacillus plantarum ) It may mean JSA22. In addition, the term "rice fermentation starter" may mean a starter fermented by inoculating lactic acid bacteria into a suspension containing rice. The rice may mean including rice flour and rice germ. The suspension may further include sugars, and may specifically include sucrose, but is not limited thereto. The rice flour included in the rice fermentation starter is characterized in that it does not contain gluten.
본 발명에 있어서, 상기 쌀 발효 스타터는 쌀가루 10 내지 15% (w/v), 쌀배아 4 내지 7% (w/v) 및 수크로스 2 내지 5% (w/v)를 포함한 것일 수 있으며, 구체적으로, 쌀가루 12.5% (w/v), 쌀배아 5% (w/v) 및 수크로스 3% (w/v)를 포함한 것일 수 있으나, 이에 제한되지 않는다.In the present invention, the rice fermentation starter may include 10 to 15% (w / v) of rice flour, 4 to 7% (w / v) of rice germ and 2 to 5% (w / v) of sucrose, Specifically, it may include rice flour 12.5% (w/v),
또한, 본 발명에 있어서, 상기 쌀 발효 스타터는 상기 현탁액을 살균하고 락토바실러스 플란타룸 균주를 접종한 후 배양하는 단계를 포함할 수 있다. 여기서, 상기 살균은 60 내지 70℃에서 30분 내지 1시간 이루어지는 것을 의미할 수 있으며, 구체적으로, 65℃에서 40분 동안 이루어지는 것을 의미할 수 있으나, 이에 제한되는 것은 아니다. 또한, 상기 배양은 25 내지 35℃에서 12 내지 36시간 동안 발효하는 것을 의미할 수 있으며, 구체적으로, 30℃에서 24시간 동안 발효한 것을 의미할 수 있으나, 이에 제한되는 것은 아니다.In the present invention, the rice fermentation starter may include sterilizing the suspension, inoculating the Lactobacillus plantarum strain, and then culturing. Here, the sterilization may mean 30 minutes to 1 hour at 60 to 70 ° C, and specifically, may mean 40 minutes at 65 ° C, but is not limited thereto. In addition, the culture may mean fermentation at 25 to 35 ° C. for 12 to 36 hours, and specifically, may mean fermentation at 30 ° C. for 24 hours, but is not limited thereto.
또한, 본 발명에 있어서, 상기 락토바실러스 플란타룸 균주는 전체 발효원료 조성물 대비 0.5 내지 2% (w/v) 로 포함된 것일 수 있으며, 구체적으로 1% (w/v)로 포함된 것일 수 있으나, 이에 제한되지 않는다.In addition, in the present invention, the Lactobacillus plantarum strain may be included in 0.5 to 2% (w / v) relative to the total fermentation raw material composition, specifically 1% (w / v). However, it is not limited thereto.
본 발명의 일 제조예에서는, 물에 쌀가루, 쌀배아 및 수크로스를 현탁시킨 현탁액을 60℃에서 40분 동안 저온 살균하고, 상기 현탁액에 락토바실러스 플란타룸 JSA22 균주를 1% (w/v) 접종한 후, 30℃에서 24시간 동안 배양하여 쌀 발효 스타터를 제조하였다(도 1).In one production example of the present invention, a suspension in which rice flour, rice germ, and sucrose were suspended in water was pasteurized at 60° C. for 40 minutes, and Lactobacillus plantarum JSA22 strain was added to the suspension by 1% (w/v). After inoculation, a rice fermentation starter was prepared by culturing at 30 ° C. for 24 hours (Fig. 1).
본 발명에서 용어, "사워도우(sourdough)"는 산성반죽이라고도 하며, 독특한 풍미가 있어 특히 호밀빵을 만들 때 필요한 반죽을 의미한다. 본 발명의 목적상, 상기 사워도우는 락토바실러스 플란타룸 JSA22로 발효한 쌀 발효 스타터가 10 내지 12% (w/w) 포함된 반죽을 의미할 수 있으나, 이에 제한되는 것은 아니다. 본 발명에서, 상기 사워도우는 반죽과 혼용하여 사용할 수 있다.In the present invention, the term "sourdough" is also referred to as acidic dough, and has a unique flavor, so it means a dough that is particularly necessary for making rye bread. For the purpose of the present invention, the sourdough may mean a dough containing 10 to 12% (w / w) of a rice fermentation starter fermented with Lactobacillus plantarum JSA22, but is not limited thereto. In the present invention, the sourdough may be used in combination with dough.
본 발명에 있어서, 상기 사워도우는 쌀가루를 포함한 것일 수 있으며, 추가로 당류, 염, 탈지유, 버터, 효모, 물로 이루어지는 군에서 선택되는 하나 이상을 포함하는 것을 의미할 수 있으나, 이에 제한되는 것은 아니다. 상기 사워도우에 포함되는 쌀가루는 글루텐이 1 내지 20% (w/w) 포함되어 있는 것을 특징으로 한다.In the present invention, the sourdough may include rice flour, and may further include one or more selected from the group consisting of sugars, salt, skim milk, butter, yeast, and water, but is not limited thereto. . Rice flour included in the sourdough is characterized in that gluten is contained in 1 to 20% (w / w).
또한, 본 발명의 목적상 상기 사워도우는 추가로 쌀 발효 스타터를 포함하는 것을 특징으로 하며, 상기 쌀 발효 스타터는 총 사워도어 조성물 대비 5 내지 70% (w/w), 구체적으로는 5 내지 50% (w/w), 보다 구체적으로는 10 내지 30% (w/w), 더욱 구체적으로는 10 내지 15% (w/w)를 포함할 수 있으나, 이에 제한되는 것은 아니다. In addition, for the purpose of the present invention, the sourdough is characterized in that it further comprises a rice fermentation starter, the rice fermentation starter is 5 to 70% (w / w), specifically 5 to 50% of the total sourdough composition % (w/w), more specifically 10 to 30% (w/w), more specifically 10 to 15% (w/w), but is not limited thereto.
본 발명에 있어서, 구체적인 상기 사워도어의 조성물은 총 사워도어 조성물 대비, 쌀가루는 45 내지 55% (w/w), 설탕 3 내지 5% (w/w), 소금 0.5 내지 1.0% (w/w), 탈지유 1.5 내지 2.0% (w/w), 이스트 1.0 내지 1.5% (w/w), 물 20 내지 30% (w/w), 버터 3 내지 5% (w/w) 및 쌀 발효 스타터 10 내지 15% (w/w)를 포함한 것일 수 있으나, 이에 제한되는 것은 아니다.In the present invention, the specific composition of the sourdough is 45 to 55% (w/w) of rice flour, 3 to 5% (w/w) of sugar, and 0.5 to 1.0% (w/w) of salt, relative to the total sourdough composition. ), skim milk 1.5-2.0% (w/w), yeast 1.0-1.5% (w/w), water 20-30% (w/w), butter 3-5% (w/w) and
본 발명에서 용어, "발효"는 미생물의 작용에 의해 유기물이 분해되는 과정을 의미한다. 본 발명의 목적상, 상기 발효는 스타터로서 사용되는 유산균, 구체적으로 락토바실러스 플란타룸 (Lactobacillus plantarum) JSA22의 작용에 의해서 유기물, 즉, 쌀이 분해되는 것을 의미할 수 있다.As used herein, the term "fermentation" refers to a process in which organic matter is decomposed by the action of microorganisms. For the purpose of the present invention, the fermentation may mean that organic matter, that is, rice, is decomposed by the action of lactic acid bacteria used as a starter, specifically Lactobacillus plantarum JSA22.
본 발명에 있어서, 상기 발효는 1차 발효, 중간 발효 및 2차 발효를 포함할 수 있다. 상기 1차 발효는 25 내지 30℃, 상대습도 70 내지 80%에서 60분 내지 120분 동안, 구체적으로 27℃, 상대습도 75%에서 90분 동안 발효한 것을 의미할 수 있고, 상기 중간 발효는 사워도우를 분할하여 가스를 빼기 위하여 가볍게 둥글리기해서 실온에서 8 내지 15분 동안, 구체적으로 10분 동안 발효하는 것을 의미할 수 있으며, 상기 2차 발효는 반죽의 부피가 제품의 90%정도 증가될 때까지 온도 32 내지 35℃, 상대습도 80 내지 90%에서 30분 내지 90분 동안, 구체적으로, 34℃, 상대습도 85%에서 60분 동안 발효한 것을 의미할 수 있으나, 이에 제한되는 것은 아니다.In the present invention, the fermentation may include primary fermentation, intermediate fermentation, and secondary fermentation. The primary fermentation may mean fermentation at 25 to 30 ° C. and 70 to 80% relative humidity for 60 minutes to 120 minutes, specifically, at 27 ° C. and 75% relative humidity for 90 minutes, and the intermediate fermentation is sour Dividing the dough and rounding it lightly to remove gas may mean fermenting at room temperature for 8 to 15 minutes, specifically 10 minutes, and the secondary fermentation is when the volume of the dough increases by about 90% of the product It may mean fermented for 30 to 90 minutes at a temperature of 32 to 35 ° C. and 80 to 90% relative humidity, specifically, for 60 minutes at 34 ° C. and 85% relative humidity, but is not limited thereto.
상기 발효가 끝난 사워도우는 d) 단계의 170 내지 180℃에서 20 내지 30분 동안, 구체적으로 25분 동안 굽는 단계를 포함할 수 있으며, 또한, 상기 굽는 단계 이후에는, e) 냉각 및 포장하는 단계를 추가로 포함할 수 있다.The fermented sourdough may include the step of baking at 170 to 180 ° C. for 20 to 30 minutes, specifically for 25 minutes in step d), and after the baking step, e) cooling and packaging may additionally include.
본 발명의 쌀빵 제조방법은, 쌀 발효 스타터 무첨가(대조군) 대비 쫄깃함 및 저장성이 증가될 수 있으며, 상기 저장성은 구체적으로, 대장균 및 곰팡이 증식이 억제되어 나타난 효과인 것일 수 있다. In the rice bread manufacturing method of the present invention, the chewiness and storability can be increased compared to the addition of rice fermentation starter (control group), and the storability may be an effect exhibited by suppressing the growth of Escherichia coli and mold.
본 발명의 일 실시예에서는, 본 발명의 쌀 발효 스타터를 포함하는 쌀빵의 품질 특성 및 관능 특성을 확인한 결과, 대조군 대비 경도 및 쫄깃함이 현저히 높았고(표 5), 색, 조직감, 기공밀도, 향, 맛 및 전반적인 기호도가 우수함을 확인하였다(표 6).In one embodiment of the present invention, as a result of confirming the quality characteristics and sensory characteristics of rice bread containing the rice fermentation starter of the present invention, the hardness and chewiness were significantly higher than those of the control group (Table 5), color, texture, pore density, flavor, It was confirmed that the taste and overall acceptability were excellent (Table 6).
또한, 본 발명의 일 실시예에서는, 상기 쌀 발효 스타터를 포함하는 쌀빵의 기능성을 확인한 결과, 대조군 대비 저장기간 중의 수분함량 및 pH의 변화가 가장 적었으며(표 7 및 표 8), 대장균 검출은 저장 6일째 대조군 대비 약 100배 낮았고(표 9), 곰팡이의 경우 육안으로 관찰되는 포자로 확인한 결과, 대조군 뿐만 아니라, 다른 균주로 발효한 쌀 발효 스타터, 구체적으로, 락토바실러스 람노서스(Lactobacillus rhamnosus) GG 첨가구인 LGG 첨가군에 비해서도 현저히 늦은 저장 6일째에 검출됨을 확인하였다(표 10 및 도 5).In addition, in one embodiment of the present invention, as a result of confirming the functionality of rice bread containing the rice fermentation starter, the change in moisture content and pH during the storage period was the smallest compared to the control group (Tables 7 and 8), and E. coli detection was On the 6th day of storage, it was about 100 times lower than that of the control group (Table 9), and in the case of mold, as confirmed by visually observed spores, rice fermentation starter fermented with other strains as well as the control group, specifically, Lactobacillus rhamnosus It was confirmed that it was detected on the 6th day of storage, which was significantly later than that of the GG-added group, the LGG-added group (Table 10 and FIG. 5).
상기 목적을 달성하기 위한 본 발명의 다른 하나의 양태는, 상기 방법에 의해 제조된 쌀빵을 제공하는 것이다.Another aspect of the present invention for achieving the above object is to provide rice bread prepared by the above method.
상기 목적을 달성하기 위한 본 발명의 다른 하나의 양태는, a) 쌀을 포함하는 현탁액을 락토바실러스 플란타룸 (Lactobacillus plantarum) 균주로 발효하여 쌀 발효 스타터를 제조하는 단계; 및 b) 상기 쌀 발효 스타터를 포함하는 단계를 포함하는 사워도우(Sourdough)의 제조방법을 제공한다.Another aspect of the present invention for achieving the above object is, a) preparing a rice fermentation starter by fermenting a suspension containing rice with a Lactobacillus plantarum strain; And b) it provides a method for producing sourdough comprising the step of including the rice fermentation starter.
상기 용어, "현탁액", "발효", "스타터" 및 "사워도우"는 상술한 바와 같다.The terms "suspension", "fermentation", "starter" and "sourdough" are as defined above.
상기 목적을 달성하기 위한 본 발명의 다른 하나의 양태는, 상기 사워도우를 포함하는 가공식품을 제공한다.Another aspect of the present invention for achieving the above object provides a processed food containing the sourdough.
상기 용어, "사워도우"는 상술한 바와 같다.The term "sourdough" is as described above.
본 발명의 용어, "가공식품"은 식품을 여러 방법으로 처리함으로써, 맛 및/또는 저장성을 높인 식품을 의미할 수 있으며, 본 발명의 목적상 상기 가공식품은 쌀을 이용한 가공식품을 의미할 수 있다.The term of the present invention, "processed food" may refer to food with improved taste and / or shelf life by processing food in various ways, and for the purpose of the present invention, the processed food may mean processed food using rice. there is.
상기 구체적인 가공식품으로는, 예를 들어 떡, 한과 등을 들 수 있으나, 이에 제한되는 것은 아니다.Examples of the specific processed food may include, for example, rice cakes and hangwa, but are not limited thereto.
본 발명의 락토바실러스 플란타룸(Lactobacillus plantarum) JSA22 균주로 발효하여 제조한 쌀 발효 스타터를 첨가한 쌀빵은, 쌀 발효 스타터 무첨가 쌀빵에 비해 경도 및 쫄깃함뿐만 아니라, 대장균 및 곰팡이 증식이 억제되어 저장성이 현저히 우수한 효과를 갖는다.Rice bread added with a rice fermentation starter prepared by fermentation with the Lactobacillus plantarum JSA22 strain of the present invention has hardness and chewiness compared to rice bread without a rice fermentation starter, as well as E. coli and mold growth are inhibited, resulting in shelf life. It has a remarkably good effect.
도 1은 본 발명의 쌀빵 제조방법에 있어서, 쌀 발효 스타터를 제조하는 공정을 도식화한 것이다.
도 2는 본 발명의 쌀빵 제조방법에 있어서, 상기 쌀 발효 스타터를 포함한 쌀빵을 제조하는 공정을 도식화한 것이다.
도 3은 쌀 발효 스타터에 포함된 유산균 유무 및 종류에 따른 사워도우의 발효 후 부품성을 비교한 사진이다.
도 4는 쌀 발효 스타터에 포함된 유산균 유무 및 종류에 따라 제조된 쌀빵의 외관 특성을 비교한 사진이다.
도 5는 락토바실러스 플란타룸(Lactobacillus plantarum) JSA22 균주를 접종한 쌀 발효 스타터를 이용하여 제조한 쌀빵의 저장 기간에 따른 곰팡이 검출을 확인한 사진이다.1 is a schematic diagram of a process for preparing a rice fermentation starter in the rice bread manufacturing method of the present invention.
Figure 2 is a schematic diagram of the process of manufacturing rice bread including the rice fermentation starter in the rice bread manufacturing method of the present invention.
Figure 3 is a photograph comparing the component properties after fermentation of sourdough according to the presence and type of lactic acid bacteria included in the rice fermentation starter.
Figure 4 is a photograph comparing the appearance characteristics of rice bread prepared according to the presence and type of lactic acid bacteria included in the rice fermentation starter.
5 is a photograph confirming mold detection according to the storage period of rice bread prepared using a rice fermentation starter inoculated with Lactobacillus plantarum JSA22 strain.
이하, 실시예를 통하여 본 발명을 보다 상세히 설명하고자 한다. 이들 실시예는 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예에 의해 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail through examples. These examples are intended to explain the present invention in more detail, and the scope of the present invention is not limited by these examples.
제조예 1. 쌀 발효 스타터 제조 방법Preparation Example 1. Rice Fermentation Starter Manufacturing Method
다음과 같은 방법으로, 쌀 발효 스타터를 제조하였다.A rice fermentation starter was prepared in the following manner.
먼저, 정선한 쌀을 시험용 분쇄기로 분쇄한 후, 50 메쉬(mesh) 체를 사용하여 통과한, 글루텐을 포함하지 않는 쌀가루를 사용하였다. 쌀배아는 로스터(MK-300, J.ROASTER, China)로 볶음 처리(180℃, 10분) 하여 사용하였다. , First, selected rice was pulverized with a test grinder, and then gluten-free rice flour passed through a 50 mesh sieve was used. Rice germ was roasted (180 ℃, 10 minutes) in a roaster (MK-300, J.ROASTER, China) and used. ,
구체적으로, 상기 쌀가루 12.5% (w/v) 및 쌀배아 5% (w/v)를 물에 현탁시킨 현탁액에 설탕을 3% (w/v) 가한 후, 65℃에서 40분간 저온 살균하였다. 이를 상온에서 방냉하고, 균질화 과정을 거친 후, 기탁번호 KACC91973P의 균주, 락토바실러스 플란타럼 JSA22(이하, JSA22라 함)를 1% (w/v) 농도로 접종하였고, 30℃에서 24시간 동안 발효시켜 쌀 발효 스타터를 제조하였다. Specifically, 3% (w/v) of sugar was added to a suspension obtained by suspending 12.5% (w/v) of rice flour and 5% (w/v) of rice germ in water, followed by pasteurization at 65° C. for 40 minutes. After cooling at room temperature and homogenization, the strain of accession number KACC91973P, Lactobacillus plantarum JSA22 (hereinafter referred to as JSA22) was inoculated at a concentration of 1% (w / v), and at 30 ° C. for 24 hours Fermented to prepare a rice fermentation starter.
또한, 락토바실러스 람노서스(Lactobacillus rhamnosus) GG (이하, LGG라 함)로 발효하여 제조한 쌀 발효 스타터를 비교예로 하였다.In addition, a rice fermentation starter prepared by fermentation with Lactobacillus rhamnosus GG (hereinafter referred to as LGG) was used as a comparative example.
제조예 2. 쌀 발효 스타터를 포함한 사워도어 제조 방법Production Example 2. Sourdough manufacturing method including rice fermentation starter
상기 제조예 1에서 JSA22 및 LGG를 발효하여 제조한 쌀 발효 스타터를 각각 10 내지 15% (w/w)로 첨가하여 각각의 사워도어를 제조하였다. 이때, 대조군(control)은 쌀 발효 스타터를 포함하지 않는 사워도어를 대조군으로 하였다 (표 1).Each sourdough was prepared by adding 10 to 15% (w / w) of rice fermentation starter prepared by fermenting JSA22 and LGG in Preparation Example 1, respectively. At this time, the control (control) was a sourdough containing no rice fermentation starter as a control (Table 1).
JSA22, LGG (g)Sourdough:
JSA22, LGG (g)
제조예 3. 쌀빵 제조 방법Preparation Example 3. Rice bread manufacturing method
본 발명의 쌀빵 제조 방법은 하기와 같이 크게 3단계를 포함한다.The rice bread manufacturing method of the present invention includes three major steps as follows.
(1) 재료 혼합 단계(1) material mixing step
쌀가루, 설탕, 소금, 탈지유(skim milk), 이스트, 물 및 쌀 발효 스타터를 먼저 혼합한 후, 수화된 버터를 넣어 2차 혼합하였다. 이때, 상기 쌀가루는 글루텐 함량이 14 내지 17% (w/w) 포함된 것을 사용하였다.Rice flour, sugar, salt, skim milk, yeast, water, and rice fermentation starter were first mixed, and then hydrated butter was added and secondly mixed. At this time, the rice flour was used with a gluten content of 14 to 17% (w / w).
(2) 발효 단계(2) fermentation step
상기 혼합단계에서 완성된 사워도어를 27℃, 상대습도 75%에서 90분 동안 1차 발효한 후, 무게 180 g씩 분할하여 가스를 빼기 위하여 가볍게 둥글리기하여 실온에서 10분 동안 중간 발효하였다.After the first fermentation for 90 minutes at 27 ° C. and 75% relative humidity, the sourdough completed in the mixing step was divided into 180 g each, lightly rounded to remove gas, and intermediate fermentation at room temperature for 10 minutes.
중간 발효된 사워도어를 한 덩어리 형태로 정형하여 빵틀에 넣고 반죽의 부피가 제품의 90%정도 증가될 때까지 온도 34℃, 상대습도 85%에서 60분 동안 2차 발효하였다. The intermediately fermented sourdough was shaped into a loaf and placed in a bread mold, followed by secondary fermentation for 60 minutes at a temperature of 34 ° C and a relative humidity of 85% until the volume of the dough increased by about 90% of the product.
(3) 소성 단계(3) firing step
2차 발효 단계를 거친 사워도어를 170~180℃에서 25분 동안 구운 후, 실온에서 냉각 및 포장하였다.The sourdough that went through the secondary fermentation was baked at 170-180 ° C for 25 minutes, then cooled and packaged at room temperature.
상기 제조한 쌀빵은 균일한 크기로 절단하고(50 mm X 50 X 10), 저밀도 폴리에틸렌(LDPE) 포장지(180 mm X 200)에 넣어 27℃ 항온항습장치에 저장하여 시료로 사용하였다. 이때, 상기 쌀빵의 제조 공정은 도 2에 나타내었다.The prepared rice bread was cut into uniform sizes (50 mm X 50 X 10), put in low-density polyethylene (LDPE) wrapping paper (180 mm X 200), stored in a constant temperature and humidity device at 27 ° C, and used as samples. At this time, the manufacturing process of the rice bread is shown in FIG.
실시예 1. 사워도어의 품질 특성Example 1. Quality Characteristics of Sourdoors
실시예 1-1. 미생물적 특성Example 1-1. Microbiological characteristics
쌀 발효 스타터에 포함된 균주의 유무 및 종류에 따른 사워도어의 미생물적 특성을 알아보았다.The microbial characteristics of sourdough were investigated according to the presence and type of strains included in the rice fermentation starter.
먼저, 유산균 수 측정은 시료를 0.9% NaCl 용액으로 10진 희석법에 의해 희석하여 MRS agar(Difco, Sparks, MD, USA)에 도말하여 37℃에서 48시간 배양한 후, 생성된 콜로니를 계수하여 log colony forming unit(CFU)/mL로 표시하였다.First, to measure the number of lactic acid bacteria, the sample was diluted with a 0.9% NaCl solution by the decimal dilution method, spread on MRS agar (Difco, Sparks, MD, USA), incubated at 37 ° C for 48 hours, and then the resulting colonies were counted and log Expressed as colony forming unit (CFU)/mL.
또한, 효모수의 측정은 시료를 0.9% NaCl 용액으로 10진 희석법에 의해 희석하여 YM agar(Difco, Sparks, MD, USA)에 도말하여 25℃에서 72시간 배양한 후, 생성된 콜로니를 계수하여 log colony forming unit(CFU)/mL로 표시하였다.In addition, the number of yeast was measured by diluting the sample with a 0.9% NaCl solution by the decimal dilution method, spreading it on YM agar (Difco, Sparks, MD, USA), incubating it at 25 ° C for 72 hours, and then counting the resulting colonies. Expressed as log colony forming unit (CFU)/mL.
상기 측정 방법에 따른 발효 후의 유산균 및 효모수는 표 2에 나타내었다.The number of lactic acid bacteria and yeast after fermentation according to the above measurement method is shown in Table 2.
상기 표 2에 나타난 바와 같이, 쌀 발효 스타터에 포함된 균주의 유무 및 종류에 따른 발효 후 사워도우의 유산균 및 효모수는 유사한 수준이였다. As shown in Table 2, the number of lactic acid bacteria and yeast in the sourdough after fermentation according to the presence and type of strains included in the rice fermentation starter was similar.
실시예 1-2. 부품성Example 1-2. componentity
쌀 발효 스타터에 포함된 균주의 유무 및 종류에 따른 발효 후 사워도우의 부품성을 알아보았다(표 3).The component properties of sourdough after fermentation according to the presence and type of strains included in the rice fermentation starter were investigated (Table 3).
상기 표 3에 나타난 바와 같이, 쌀 발효 스타터에 포함된 균주의 유무 및 종류에 따른 발효 후 JSA22 포함 사워도우의 부품성은 대조군 대비 약간 낮은 수준이었으나, LGG 포함 사워도우의 부품성에 비해서는 높은 수준이었다(도 3).As shown in Table 3, the componentity of the sourdough containing JSA22 after fermentation according to the presence and type of strains included in the rice fermentation starter was slightly lower than that of the control group, but higher than that of sourdough containing LGG ( Fig. 3).
실시예 2. 쌀빵의 품질 특성Example 2. Quality Characteristics of Rice Bread
실시예 2-1. 외관 특성 - 비체적Example 2-1. Appearance Characteristics - Specific Volume
쌀 식빵의 외관특성인 비체적 특성 평가는 쌀빵을 구운 후 1시간 동안 식힌 다음 무게(g)를 재었고, 부피(ml)는 AACC(2000) 방법에 의한 종자치환법으로 측정하여, 이로부터 비체적(specific volume; mL/g)을 계산하였고, 하기 표 4에 나타내었다.To evaluate the specific volume characteristics, which is the external appearance of rice bread, the weight (g) was measured after the rice bread was baked and cooled for 1 hour, and the volume (ml) was measured by the seed replacement method according to the AACC (2000) method. The specific volume (mL/g) was calculated and is shown in Table 4 below.
(mL)volume
(mL)
(g)weight
(g)
(mL/g)specific volume
(mL/g)
상기 표 4에 나타난 바와 같이, 쌀 발효 스타터에 포함된 균주의 유무 및 종류에 따른 사워도우로 제조한 쌀빵에 있어서 비체적은 큰 차이를 보이지 않았으며, 즉 유사한 수준의 빵의 가벼운(부드러움) 정도를 보였다(도 4).As shown in Table 4, the specific volume did not show a significant difference in the rice bread made with sourdough according to the presence and type of strains included in the rice fermentation starter, that is, the lightness (softness) of the bread at a similar level. was seen (FIG. 4).
실시예 2-2. 텍스처 특성Example 2-2. texture properties
텍스처 측정은 쌀빵의 중심부분을 2.0 Х 2.0 Х 1.0 cm 크기로 일정하게 잘라 Texture analyzer(TA.XT plus, Stable Micro Systems Ltd., Surrey, UK)를 이용하였다. Texture Profile Analyzer(TPA)는 50 mm cylindrical probe를 장착한 다음 80% deformation으로 하였고, 측정 조건은 test speed 0.5 mm/s, probe speed 1.5mm/s, trigger force 1.0 g으로 하여 경도(hardness), 접착성(adhesiveness), 탄력성(springiness), 응집성(cohesivenss), 점성(gumminess), 씹힘성(chewiness) 및 탄성(resilieness)을 측정하였고, 하기 표 5에 나타내었다.For texture measurement, a texture analyzer (TA.XT plus, Stable Micro Systems Ltd., Surrey, UK) was used by cutting the central part of the rice bread to a size of 2.0 Х 2.0 Х 1.0 cm. The Texture Profile Analyzer (TPA) was equipped with a 50 mm cylindrical probe and subjected to 80% deformation, and the measurement conditions were test speed 0.5 mm/s, probe speed 1.5 mm/s, trigger force 1.0 g, and Adhesiveness, springiness, cohesivenss, gumminess, chewiness and resilieness were measured and are shown in Table 5 below.
nessAdhesive
ness
nessCohesive
ness
nessChewi
ness
essResilien
ess
상기 표 5에 나타난 바와 같이, 대조군 대비 쌀 발효 스타터 첨가구에서 경도 및 쫄깃함이 우수하였으며, 특히, JSA22 첨가구에서 현저히 우수함을 확인하였다.As shown in Table 5, it was confirmed that hardness and chewiness were excellent in the rice fermentation starter addition group compared to the control group, and in particular, remarkably excellent in the JSA22 addition group.
실시예 2-3. 관능 특성Example 2-3. sensory properties
관능 특성은 농촌진흥청 국립식량과학원 연구원 20명을 대상으로 실험목적 및 평가항목에 대해 충분히 설명한 다음 실시하였다. 시료는 색과 향이 없는 용기에 일정량을 담고 물과 함께 제공하였으며, 평가항목은 색, 기공밀도, 조직감, 향, 맛, 전반적 기호도에 대하여, 항목별 아주 나쁜 것은 1점, 보통은 5점, 가장 좋은 것은 9점으로 점수화하는 9점 척도법으로 평가하여, 하기 표 6에 나타내었다.Sensory characteristics were conducted after sufficiently explaining the purpose of the experiment and evaluation items to 20 researchers at the National Institute of Food Science, Rural Development Administration. Samples were put in a certain amount in a container without color and fragrance and provided with water. The evaluation items were color, pore density, texture, aroma, taste, and overall acceptability. The good ones were evaluated on a 9-point scale with 9 points, and are shown in Table 6 below.
상기 표 6에 나타난 바와 같이, 대조군 대비 쌀 발효 스타터 첨가구에서 조직감 및 향의 기호도가 우수하였으며, 특히 JSA22 첨가구는 색, 기공밀도, 조직감, 향, 맛 및 전반적 기호도가 우수함을 확인하였다.As shown in Table 6, the texture and flavor preference was excellent in the rice fermentation starter added group compared to the control group, and in particular, the JSA22 additive group was confirmed to be excellent in color, pore density, texture, aroma, taste and overall acceptability.
실시예 3. 쌀빵의 저장성Example 3. Storage of rice bread
실시예 3-1. 수분함량, pH 및 산도Example 3-1. Moisture content, pH and acidity
쌀빵의 수분함량은 껍질부분을 제외한 중간 부분에서 시료를 일정하게 1 g씩 취하여 수분측정기(MAXB45 Moisture Analyzer, Shimadzu Corpo, Kyoto, Japan)를 이용하여 120℃에서 할로겐 가열방식으로 측정하였으며, 하기 표 7에 나타내었다.The moisture content of rice bread was measured by a halogen heating method at 120 ° C. using a moisture meter (MAXB45 Moisture Analyzer, Shimadzu Corpo, Kyoto, Japan) by taking samples of 1 g at a constant rate from the middle part except for the shell part, Table 7 below shown in
(unit:%)쌀빵의 pH는 시료 2 g을 증류수로 10배 희석한 후, 균일하게 혼합하여 실온에서 pH 미터(Metrohm 691, Metrohm, Herisau, Switzerland)로 측정하였다. 산도는 시료액 10 mL에 0.1 N NaOH를 첨가하면서 pH가 8.3이 될 때까지의 소비된 NaOH의 mL를 측정하여 젖산(lactic acid) 함량으로 산출하였으며, 하기 표 8에 나타내었다.The pH of (unit:%) rice bread was measured with a pH meter (Metrohm 691, Metrohm, Herisau, Switzerland) at room temperature after diluting 2 g of the
상기 표 7 및 표 8에 나타난 바와 같이, 쌀빵의 8일 동안의 저장 기간 중 수분함량 및 pH는 JSA22 첨가구에서 시간이 지남에 따른 변화가 가장 작았다. As shown in Tables 7 and 8, the moisture content and pH of rice bread during the storage period of 8 days showed the smallest change over time in the JSA22 added group.
실시예 3-2. 대장균 및 곰팡이 검출Example 3-2. Escherichia coli and mold detection
대장균군수 측정은 쌀빵 시료를 0.9% NaCl 용액으로 10진 희석법에 의해 희석하여 Desoxycholate agar(Difco, Sparks, MD, USA)에 도말하여 37℃에서 48시간 배양한 후, 생성된 콜로니를 계수하여 log colony forming unit(CFU)/mL로 표시하였으며, 하기 표 9에 나타내었다.For the measurement of coliform count, a rice bread sample was diluted with a 0.9% NaCl solution by the decimal dilution method, spread on Desoxycholate agar (Difco, Sparks, MD, USA), incubated at 37℃ for 48 hours, and then the colonies produced were counted and log colony It was expressed as forming unit (CFU)/mL and is shown in Table 9 below.
(unit: Log CFU/g)상기 표 9에 나타난 바와 같이, 대장균의 경우, 대조구에서는 5일째 검출된 반면, 유산균 처리구에서는 6일째 검출되었으며, 특히, 저장 6일째 JSA22 첨가구의 경우, 대조구 대비 약100배, LGG 첨가구 대비 1000배 낮게 나타났음을 확인하였다.(unit: Log CFU/g) As shown in Table 9 above, in the case of Escherichia coli, it was detected on the 5th day in the control group, whereas it was detected on the 6th day in the lactic acid bacteria treatment group. It was confirmed that it appeared 1000 times lower than the pear and LGG added group.
또한, 곰팡이 수 측정은 쌀빵 시료를 0.9% NaCl 용액으로 10진 희석법에 의해 희석하여 3M Petrifilm에 접종하여 누름판으로 눌러준 후, 25℃에서 48시간 배양한 후, 생성된 콜로니를 계수하여 log colony forming unit(CFU)/mL로 표시하였으며, 하기 표 10에 나타내었다.In addition, the number of molds was measured by diluting the rice bread sample with 0.9% NaCl solution by the decimal dilution method, inoculating it on 3M Petrifilm, pressing it with a pressure plate, incubating it at 25 ° C for 48 hours, and counting the resulting colonies to log colony forming. It was expressed as unit (CFU)/mL and is shown in Table 10 below.
JSA22(A)
JSA22
LGG(B)
LGG
(unit: Log CFU/g)상기 표 10에 나타난 바와 같이, 곰팡이의 경우는 저장기간 중 육안으로 관찰되는 포자 유무를 관찰하여 확인한 결과, 대조구 및 LGG 첨가구에서는 저장 4일째부터 관찰되는 반면, JSA22 첨가구에서는 저장 6일째 검출되었다(도 5). (unit: Log CFU/g) As shown in Table 10 above, in the case of mold, the presence or absence of spores observed with the naked eye during the storage period was confirmed. In addition, it was detected on the 6th day of storage (FIG. 5).
이상의 설명으로부터, 본 발명이 속하는 기술분야의 당업자는 본 발명이 그 기술적 사상이나 필수적 특징을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다. 이와 관련하여, 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적인 것이 아닌 것으로서 이해해야만 한다. 본 발명의 범위는 상기 상세한 설명보다는 후술하는 특허 청구범위의 의미 및 범위 그리고 그 등가 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, the embodiments described above should be understood as illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.
Claims (24)
b) 상기 쌀 발효 스타터 및 쌀가루를 포함하는 사워도우(Sourdough)를 제조하는 단계로서,
상기 쌀 발효 스타터는 총 사워도우 조성물 대비 5 내지 50% (w/w) 포함하는 것인, 사워도우를 제조하는 단계;
c) 상기 사워도우를 발효하는 단계; 및
d) 상기 발효된 사워도우를 굽는 단계를 포함하는, 쌀빵의 제조방법으로서,
상기 쌀빵은 저장성이 증가된 것인, 쌀빵의 제조방법.
a) preparing a rice fermentation starter by fermenting a suspension containing rice with Lactobacillus plantarum JSA22 strain having accession number KACC91973P;
b) preparing a sourdough containing the rice fermentation starter and rice flour,
Preparing sourdough, wherein the rice fermentation starter contains 5 to 50% (w / w) of the total sourdough composition;
c) fermenting the sourdough; and
d) a method for producing rice bread comprising the step of baking the fermented sourdough,
The rice bread is a method for producing rice bread, in which the shelf life is increased.
The method of claim 1, wherein the rice in step a) includes rice flour and rice germ.
The method of claim 2, wherein the rice flour does not contain gluten.
The method of claim 1, wherein the suspension in step a) further comprises sugars.
The method of claim 1, wherein step a) sterilizes a suspension in which rice flour, rice germ, and sucrose are suspended in water, and inoculates Lactobacillus plantarum strain JSA22 having accession number KACC91973P, followed by culturing. Method for producing phosphorus and rice bread.
The method of claim 5, wherein the sterilization is performed at 60 to 70° C. for 30 minutes to 1 hour.
The method of claim 5, wherein the culture is fermented at 25 to 35 ° C for 12 to 36 hours.
The method of claim 1, wherein the Lactobacillus plantarum strain JSA22 having the accession number KACC91973P is included in an amount of 0.5 to 2% (w/v) relative to the total fermented raw material composition.
The method of claim 1, wherein the rice flour contains 1 to 20% (w/w) gluten.
The method of claim 1, wherein the rice flour is included in 45 to 55% (w / w) compared to the total sourdough composition.
The method of claim 1, wherein the sourdough in step b) further comprises at least one selected from the group consisting of sugar, salt, skim milk, butter, yeast, and water.
The method of claim 1, wherein the fermentation in step c) includes primary fermentation, intermediate fermentation, and secondary fermentation.
The method of claim 12, wherein the primary fermentation is performed at 25 to 30° C. and 70 to 80% relative humidity for 60 to 120 minutes.
The method of claim 12, wherein the intermediate fermentation is performed by dividing the sourdough, rounding it lightly to remove gas, and fermenting at room temperature for 8 to 15 minutes.
The rice bread according to claim 12, wherein the secondary fermentation is fermented for 30 to 90 minutes at a temperature of 32 to 35 ° C and a relative humidity of 80 to 90% until the volume of the sourdough increases by about 90% of the product. Manufacturing method of.
The method of claim 1, wherein the baking step of step d) is performed at 170 to 180° C. for 20 to 30 minutes.
The method of claim 1, further comprising the steps of e) cooling and packaging.
The method of claim 1, wherein the rice bread has increased chewiness.
The method for producing rice bread according to claim 1, wherein the storability is an effect obtained by inhibiting the growth of Escherichia coli and mold.
Rice bread prepared by the method of any one of claims 1 to 19, wherein the rice bread has increased shelf life.
The rice bread according to claim 20, wherein the rice bread has increased chewiness.
The rice bread according to claim 20, wherein the shelf life is an effect obtained by inhibiting the growth of Escherichia coli and mold.
b) 상기 쌀 발효 스타터 및 쌀가루를 포함하는 단계를 포함하는, 사워도우(Sourdough) 제조방법으로서,
상기 쌀 발효 스타터는 총 사워도우 조성물 대비 5 내지 50% (w/w) 포함하고,
상기 사워도우는 쌀빵 제조시 쌀빵의 저장성이 증가되는 것인, 사워도우 제조방법.
a) preparing a rice fermentation starter by fermenting a suspension containing rice with Lactobacillus plantarum strain JSA22 having accession number KACC91973P; and
b) a sourdough manufacturing method comprising the step of including the rice fermentation starter and rice flour,
The rice fermentation starter contains 5 to 50% (w / w) of the total sourdough composition,
The sourdough is a method for producing sourdough, in which the shelf life of rice bread is increased during rice bread production.
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