KR20220167945A - TRAP-1 inhibitor - Google Patents

Abstract

The present specification provides compounds that inhibit TRAP1 or pharmaceutically acceptable salts thereof. In the case, the compound or a pharmaceutically acceptable salt thereof may inhibit the binding of TRAP1 to a client protein. In addition, the present specification provides a pharmaceutical composition comprising the compounds that inhibit TRAP1 or the pharmaceutically acceptable salts thereof, and uses thereof.

Description

TRAP-1 inhibitor {TRAP-1 inhibitor}

The present specification relates to compounds and uses thereof for TRAP1 inhibition.

TRAP1 (Tumor necrosis factor receptor-associated protein-1) is a paralog of heat shock protein-90 (HSP90), a chaperone protein, and is a mitochondrial protein that exists only in mitochondria. The present inventors synthesized a compound for TRAP1 inhibition, inhibited TRAP1 using the compound, and further recognized the use of the compound as a pharmaceutical composition for treating disease.

In one embodiment, the present specification provides a compound that inhibits TRAP1 or a pharmaceutically acceptable salt thereof.

In yet another embodiment, the present specification provides a compound that inhibits the binding of TRAP1 to a client protein or a pharmaceutically acceptable salt thereof.

In another embodiment, the present specification provides a pharmaceutical composition comprising a compound that inhibits TRAP1 or a pharmaceutically acceptable salt thereof.

In another embodiment, the present specification provides a method for treating a disease comprising administering a pharmaceutical composition comprising a compound that inhibits TRAP1 or a pharmaceutically acceptable salt thereof to a subject in need thereof.

In the present specification, a compound represented by Formula 1 or a pharmaceutically acceptable salt thereof is provided:

[Formula 1]

At this time, L includes (CH ₂ ) _n ,

Wherein n is an integer of 7 or more and 40 or less,

A provides a compound selected from methyl, substituted or unsubstituted aryl, substituted or unsubstituted cycloalkyl, and substituted or unsubstituted heterocyclyl, or a pharmaceutically acceptable salt thereof.

In this case, A is one or more selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy A compound selected from substituted or unsubstituted aryl, cycloalkyl and heterocyclyl, or a pharmaceutically acceptable salt thereof is provided.

In this case, A is a substituted or unsubstituted aryl,

The substituted or unsubstituted aryl is selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy A compound that is phenyl substituted or unsubstituted with one or more, or a pharmaceutically acceptable salt thereof is provided.

,

,

,

,

,

,

,

,

및

로 구성된 군으로부터 선택되는 화합물, 또는 이의 약학적으로 허용가능한 염을 제공한다.At this time, the A is

,

,

,

,

,

,

,

,

and

Provides a compound selected from the group consisting of, or a pharmaceutically acceptable salt thereof.

In this case, A is a substituted or unsubstituted aryl,

The substituted or unsubstituted aryl is selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy Provided are naphthalene substituted or unsubstituted with one or more compounds, or pharmaceutically acceptable salts thereof.

인 화합물, 또는 이의 약학적으로 허용가능한 염을 제공한다.At this time, the A is

A phosphorus compound, or a pharmaceutically acceptable salt thereof.

In this case, A is a substituted or unsubstituted aryl,

The substituted or unsubstituted aryl is selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy It provides a compound that is benzodioxole substituted or unsubstituted with one or more, or a pharmaceutically acceptable salt thereof.

또는

인 화합물, 또는 이의 약학적으로 허용가능한 염을 제공한다.At this time, the A is

or

A phosphorus compound, or a pharmaceutically acceptable salt thereof.

In this case, A is a substituted or unsubstituted cycloalkyl,

The substituted or unsubstituted cycloalkyl is selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy A cyclohexyl substituted or unsubstituted compound with at least one selected compound or a pharmaceutically acceptable salt thereof is provided.

In this case, A is an unsubstituted cyclohexyl compound, or a pharmaceutically acceptable salt thereof.

In this case, A is a substituted or unsubstituted heterocyclyl,

The substituted or unsubstituted heterocyclyl is halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy Provided is a pyrrolidine or chromane compound substituted or unsubstituted with one or more selected from, or a pharmaceutically acceptable salt thereof.

In this case, A is pyrrolidine substituted with one or more selected from oxycarbonyl, C1-5 alkyl and =O, or a pharmaceutically acceptable salt thereof.

인 화합물, 또는 이의 약학적으로 허용가능한 염을 제공한다.At this time, the A is

A phosphorus compound, or a pharmaceutically acceptable salt thereof.

In this case, A is chroman-2-yl substituted with one or more selected from C1-5 alkyl and hydroxyl, or a pharmaceutically acceptable salt thereof.

인 화합물, 또는 이의 약학적으로 허용가능한 염을 제공한다.At this time, the A is

A phosphorus compound, or a pharmaceutically acceptable salt thereof.

In this case, n is an integer of 9 or more, or a pharmaceutically acceptable salt thereof is provided.

In this case, a TRAP1 inhibitor comprising the compound represented by Formula 1 or a pharmaceutically acceptable salt thereof is provided.

At this time, the TRAP1 inhibitor provides an inhibitor characterized in that it binds to CBS.

In this case, a TRAP1-client protein binding inhibitor comprising the compound represented by Formula 1 or a pharmaceutically acceptable salt thereof is provided.

TRAP-1 can be inhibited by using the compound provided herein or a pharmaceutically acceptable salt thereof.

Binding between TRAP-1 and a client protein can be inhibited by using the compound provided herein or a pharmaceutically acceptable salt thereof.

A pharmaceutical composition comprising a compound or a pharmaceutically acceptable salt thereof provided herein may be used to treat a specific disease related to the TRAP-1 mechanism. For example, a specific disease can be treated by administering the compound provided herein or a pharmaceutically acceptable salt thereof to a subject in need thereof.

Figure 1a is the overall structure of zTRAP1 dimer, MitoQ and AMPPNP combined. Protomer A and protomer B are shown in cyan and magenta, respectively.
Figure 1b details the recognition of MitoQ by TRAP1. Sidechains of residues can be seen interacting with MitoQ. Fo-Fc maps (grey mesh) were calculated in the absence of MitoQ.
Figure 1c shows the structure of the MitoQ binding pocket. Top shows Ub, bottom shows the TPP binding pocket.
Figure 2 shows the crystal structure of the binding of TRAP1 and MitoQ.
Figure 3 shows the structure of alkyl-TPP.
Figure 4 shows the results of the CBS (Client binding site) binding force analysis of TRAP1 of alkyl-TPP.
Figure 5 shows the antioxidant-TPP conjugate structure.
Figure 6 shows the results of analysis of the CBS binding force of the antioxidant-TPP conjugate in TRAP1.
Figure 7 shows the structure of other synthetic compounds.
Figure 8 shows the results of analysis of CBS binding force in TRAP1 of other synthetic compounds.
9a shows the result of analyzing the TRAP1 ATPase activity of alkyl-TPP.
Figure 9b shows the results of analyzing the ATP binding pocket of TRAP1 of alkyl-TPP. mP stands for millipolarization.
Figure 10 shows the results of inhibition of TRAP1 and Hsp90 in cancer cells.
Figure 11 shows the TRAP1 ATPase activity analysis results according to the linker length.
Figure 12 shows the effect of antioxidant-TPP conjugates on cells.
13 shows the effects of MitoQS, Mito-VitEL, and Mito-CPS on Hsp90 and TRAP1.
Figure 14 shows the TRAP1 ATPase activity analysis results of other synthetic materials.
15 shows TRAP1 inhibition results of other synthetic materials.
16 shows the results of the tumor growth inhibitory effect of other synthetic materials in vivo.
Figure 17 shows the results of Western blotting of tumors.

term definition

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. All publications, patents and other references mentioned herein are incorporated by reference in their entirety.

Hereinafter, specific details of the invention are disclosed.

TRAP-1

TRAP-1 is one of the paralogs of HSP90 and is a mitochondrial protein that exists only in mitochondria. The hsp90 including the TRAP-1 has a structure in which two protomers are bound. At this time, each unit is referred to as a first unit and a second unit. At this time, when a client protein specific to hsp90 is bound, Hsp90 units that have been separated from each other are brought closer to each other and ATP is bound and decomposed, thereby forming the three-dimensional structure of the client protein. Each unit is composed of an N-terminal domain, a middle domain, and a C-terminal domain. The N-terminal site is a site where ATP is bound to produce energy for the activity of hsp90s. The stop site is a binding site for each hsp90s-specific client protein. The C-terminal site is a site where two units are connected. hsp90s is characterized by very high homology of the N-terminal region between paralogs, but low homology of the interruption region. Accordingly, compounds targeting the N-terminal site may act non-selectively on the hsp90 paralog, and compounds targeting low homology sites may act selectively on the hsp90 paralog. . The hsp90 includes Hsp90-α1, Hsp90-α2, and Hsp90-β, which are cytoplasmic hsp90s, unless otherwise specified.

In addition, it is known that when the TRAP1 is inhibited, the expression of SIRT3 and SDHB is reduced (see Interplay between TRAP1 and Sirtuin-3 Modulates Mitochondrial Respiration and Oxidative Stress to Maintain Stemness of Glioma Stem Cells. Cancer Res 79, 1369-1382). Accordingly, in order to determine whether TRAP-1 is inhibited, the expression of SIRT3 and SDHB can be confirmed in the present specification.

aryl

The term "aryl", as used herein, includes substituted or unsubstituted single-ring aromatic groups where each atom of the ring is carbon. Preferably, the ring is a 5- to 7-membered ring, more preferably a 6-membered ring. The term "aryl" also includes polycyclic ring systems having two or more cyclic rings wherein two adjoining rings share two or more carbons and at least one of said rings is aromatic; Other cyclic rings can be cycloalkyls, cycloalkenyls, cycloalkynyls, aryls, heteroaryls, and/or heterocyclyls. Aryl groups include benzene, naphthalene, phenanthrene, phenol, aniline, and the like.

cycloalkyl

A "cycloalkyl" group is a fully saturated cyclic hydrocarbon. "Cycloalkyl" includes monocyclic and bicyclic rings. Unless otherwise defined, monocyclic cycloalkyl groups generally have from 3 to about 10 carbon atoms, and more typically from 3 to 8 carbon atoms. The second ring of the bicyclic cycloalkyl may be selected from saturated, unsaturated and aromatic rings. Cycloalkyls are bicyclic molecules that contain 1, 2 or 3 or more atoms shared between the two rings.

heterocyclyl

The term "heterocyclyl" refers to a substituted or unsubstituted non-aromatic ring structure, preferably a 3- to 10-membered ring ring, more preferably a 3- to 7-membered ring structure, and a ring structure thereof contains at least one heteroatom, preferably 1 to 4 heteroatoms, more preferably 1 or 2 heteroatoms. The terms "heterocyclyl" and "heterocyclic" also refer to a polycyclic ring system having two or more cyclic rings wherein two adjoining rings share two or more carbons and at least one of said rings is heterocyclic. and, illustratively, the other cyclic rings can be cycloalkyls, cycloalkenyls, cycloalkynyls, aryls, heteroaryls, and/or heterocyclyls. Heterocyclyl groups include, for example, piperidine, piperazine, pyrrolidine, morpholine, lactones, lactams, and the like.

hydroxyalkyl

"Hydroxyalkyl" is an alkyl group having at least one hydroxy substituent, e.g., a linear monovalent hydrocarbon radical containing 1 to 6 carbon atoms or 3 to 6 carbon atoms, substituted with one or two hydroxy groups. means a branched monovalent hydrocarbon radical comprising, provided that, when two hydroxyl groups are present, they are not both present on the same carbon atom. Specifically, hydroxymethyl, 2-hydroxyethyl, 2-hydroxypropyl, 3-hydroxypropyl, 1-(hydroxymethyl)-2-methylpropyl, 2-hydroxybutyl, 3-hydroxybutyl, 4-hydroxybutyl, 2,3-dihydroxypropyl, 1-(hydroxymethyl)-2-hydroxyethyl, 2,3-dihydroxybutyl, 3,4-dihydroxybutyl and 2-( hydroxymethyl)-3-hydroxypropyl and the like.

pharmaceutically acceptable

As used herein, the term "pharmaceutically acceptable" means that, within the scope of reasonable medical judgment, excessive toxicity, irritation, allergic reaction, or other problems or side effects are not exhibited. Accordingly, the term is used for compounds, materials, compositions, and/or dosage forms that are suitable for contact with the tissue of a subject and that have a reasonable benefit/risk ratio.

Compound of Formula 1

One. Formula 1

[Formula 1]

The present specification provides a compound having the structure of Formula 1 above.

A may be selected from methyl, substituted or unsubstituted aryl, substituted or unsubstituted cycloalkyl, and substituted or unsubstituted heterocyclyl.

L comprises (CH ₂ ) _n ;

In (CH ₂ ) _n , n may be an integer of 7 or more and 50 or less. In one embodiment, it may be an integer of 7 or more and 40 or less. In the above (CH ₂ ) _n , n is 7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24, It may be any one integer of 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, and 40. However, it is not limited thereto. In the present specification, the L may be understood as a concept corresponding to a linker or a linker.

2. structure of A

One) methyl

In one embodiment, A may be methyl.

2) substituted or unsubstituted aryl

In one embodiment, A may be a substituted or unsubstituted aryl.

,

,

,

,

,

,

,

,

및

로 구성된 군으로부터 선택될 수 있다. A is a specific example, from halogen, =O, hydroxy, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy It may be an aryl substituted or unsubstituted with one or more selected ones. A is a more specific embodiment, halogen, =O, hydroxy, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 It may be phenyl unsubstituted or substituted with one or more selected from alkoxy. A is a more specific embodiment,

,

,

,

,

,

,

,

,

and

It may be selected from the group consisting of.

일 수 있다.In another specific embodiment, A is halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 It may be naphthalene unsubstituted or substituted with one or more selected from alkoxy. In a more specific embodiment, A may be naphthalen-2-yl substituted with one or more selected from C1-5 alkyl and =O. The above A is a more specific embodiment

can be

또는

일 수 있다.In another specific embodiment, A is halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 It may be a substituted or unsubstituted benzodioxole substituted or unsubstituted with one or more selected from alkoxy. In a more specific embodiment, A may be 1,3-benzodioxole or 2,2-difluoro-1,3-benzodioxole. A is a specific embodiment,

or

can be

However, A is not limited thereto.

3) substituted or unsubstituted cycloalkyl

In one embodiment, A may be a substituted or unsubstituted cycloalkyl.

In a specific embodiment, A is selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy It may be a monocyclic cycloalkyl having 3 to 10 carbon atoms unsubstituted or substituted with one or more selected ones. In a more specific embodiment, A is halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 It may be cyclohexyl unsubstituted or substituted with one or more selected from alkoxy. However, A is not limited thereto.

4) Substituted or unsubstituted heterocyclyl

In one embodiment, A may be a substituted or unsubstituted heterocyclyl.

또는

일 수 있다.In a specific embodiment, A is selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy It may be pyrrolidine or chroman, which is unsubstituted or substituted with one or more selected ones. In a more specific embodiment, A may be pyrrolidine substituted with one or more selected from oxycarbonyl, C1-5 alkyl, and =O. In another specific embodiment, A may be chroman-2-yl substituted with one or more selected from C1-5 alkyl and hydroxyl. A is a specific embodiment,

or

can be

3. Structure of L

In Formula 1, L may have a structure having a specific length. In one embodiment, it may have a structure including alkyl, alkenyl, alkynyl and/or ethyleneoxy. In a specific embodiment, L may have a structure including (CH ₂ ) _n . In this case, n may be an integer of 7 or more and 40 or less in one embodiment. The n is a factor that determines the length of the connecting portion or linker in the compound of the present application, and may play an important role in binding the compound having the structure of Formula 1 to TRAP1.

However, the structure of L is not limited thereto. In this case, the length of L may be 10, 15, 20 or 25 angstroms or more in one embodiment. At this time, in one embodiment, the length of the L may be a structure with a maximum value of 50, 60, 70, 80 or 90 angstroms. However, the maximum value of the L length is not limited thereto. In one embodiment, L may have a structure including alkyl, alkenyl, alkynyl and/or ethyleneoxy having the above length. In a specific embodiment, the L may be an alkyl structure having the above length.

One) Binding capacity as a function of distance from TPP

Referring to the binding crystal structure of TRAP1 MitoQ (used interchangeably with SMX in this specification) in FIG. 2, the distance between the two protomers of TRAP1 is about 25 Å, so the distance between the Ub moiety and the TPP moiety of MitoQ is appropriate. It can be confirmed that an effective combination is possible. In addition, further referring to the experimental results of alkyl-TPP (the structure of alkyl-TPP is shown in FIG. 3), it can be confirmed that competitive binding with SB-TM2 is possible from octyl-TPP.

That is, in the structure of the compound of the present application, when the length of the alkyl chain (CH ₂ ) _n bonded to TPP is greater than or equal to C8, the compound can effectively bind to TRAP1. More specifically, when the length of the alkyl chain bound to TPP has a size of C8 or more, the compound can bind to the CBS (client binding site) of TRAP1. In addition, this binding force increases as the distance to TPP increases, for example, as the length of the alkyl chain increases. In one embodiment, additionally, when comparing the bonding strength with SMX (MitoQ), dodecyl-TPP, tetradecyl-TPP, and hexadecyl-TPP bonding strength is shown to be superior to SMX. In addition, in one embodiment, in particular, hexadecyl-TPP, which has the longest alkyl chain, was confirmed to have twice as strong binding force as SMX (see Table 1 and FIG. 4).

TRAP1 binding affinity analysis result of alkyl-TPP IC ₅₀ (μM), FP analysis using SB-TM2 Drug IC ₅₀ Active versus SMX TPP-1 N.D. N.D. TPP-2 N.D. N.D. TPP-4 N.D. N.D. TPP-6 18.85 ± 5.39 0.03 TPP-8 2.572 ± 0.258 0.24 TPP-10 0.6931 ± 0.0578 0.90 TPP-12 0.3769 ± 0.0354 1.65 TPP-14 0.3835 ± 0.0392 1.62 TPP-16 0.3091 ± 0.0260 2.01 SMX 0.6227 ± 0.0375 One

2) Antioxidant-TPP conjugate

Experimental results using antioxidant and TPP conjugates (MitoQ, Mito-CP, SkQ1, Mito-VitEL ^L , Mito-TEMPO, MitoQ ^s , Mito-VitE, Mito-CP ^s structures are shown in FIG. 5) , it can be seen that Mito-TEMPO, Mito-CP ^s Mito-VitE having a short linker have no binding ability to TRAP1 (see Table 2 and FIG. 6). That is, it can be confirmed that the proper distance of the linker is essential for TRAP1 binding.

TRAP1 binding force analysis result of antioxidant-TPP conjugate IC ₅₀ (μM), FP analysis using SB-TM2 Drug IC ₅₀ Active versus SMX SkQ1 0.6959 ± 0.0554 1.18 Mito-CP 1.074 ± 0.0818 0.77 Mito-VitE ^L 0.9187 ± 0.0714 0.90 Mito-TEMPO N.D. N.D. Mito-CP ^S N.D. N.D. Mito-VitE N.D. N.D. SMX 0.824 ± 0.0512 One

3) other synthetic compounds

Referring to the results of binding experiments using other compounds in which n is 10 (structures of synthetic materials are shown in FIG. 7), it can be confirmed that when TPP and various compound structures are linked through a hydrocarbon having a specific length, they have binding force to TRAP1. Yes (see Tables 3, 4 and Figure 8).

TRAP1 binding force analysis results of other synthetic compounds IC ₅₀ (μM), FP analysis using SB-TM2 Drug IC ₅₀ Active versus SMX SB-U005 0.3853 ± 0.0835 2.051907604 SB-U009 0.9086 ± 0.1363 0.87012987 SB-U011 0.6838 ± 0.0729 1.156186019 SB-U012 0.6010 ± 0.0906 1.31547421 SMX 0.7906 ± 0.0512 One

TRAP1 binding force analysis results of other synthetic compounds IC50 (μM), FP analysis using SB-TM2 Drug IC50 Active versus SMX SB-U014 0.8046 ± 0.0994 0.50 SB-U015 1.835 ± 0.2849 0.22 SB-K001 0.1658 ± 0.0230 2.45 SB-B001 2.966 ± 1.055 0.14 SB-B002 0.1451 ± 0.13 2.80 SMX 0.4063 ± 0.0389 One

That is, what is important for the compound having the structure of Formula 1 to bind to TRAP1 is that n has an integer of at least 7 or more. In addition, TRAP1 binding force increases as the value of n increases, and accordingly, n may have an integer of up to 20, 30, 40, or 50, in one embodiment. However, the maximum value of n is not limited thereto.

4. Salts of Formula 1

The compounds disclosed herein include salt forms thereof. At this time, the salt includes a pharmaceutically acceptable salt. Salts disclosed herein include acid addition salts or basic addition salts. Exemplary acids forming the salt include hydrochloric acid, sulfuric acid, phosphoric acid, glycolic acid, lactic acid, pyruvic acid, citric acid, succinic acid, glutaric acid, and the like, and exemplary bases forming the salt include lithium, sodium, potassium, calcium, magnesium , methylamine, trimethylamine, and the like. However, it is not limited thereto and can be easily selected by those skilled in the art.

5. Examples of specific compounds

Specific examples of compounds disclosed herein No. compound name One

옥틸트리페닐포스포늄

Octyltriphenylphosphonium 2

Decyltriphenylphosphonium 3

dodecyltriphenylphosphonium 4

tetradecyltriphenylphosphonium 5

Hexadecyltriphenylphosphonium 6

(10-(3-bromo-4,5,6-trimethoxy-2-methylphenyl)decyl)triphenylphosphonium 7

(10-(2-bromo-5-hydroxy-3,4-dimethoxy-6-methylphenyl)decyl)triphenylphosphonium 8

(10-(3-bromo-4,5,6-trimethoxy-2-methylphenyl)decyl)triphenylphosphonium 9

(10-(2-bromo-3,4,5-trimethoxy-6-methylphenyl)decyl)triphenylphosphonium 10

Triphenyl(10-(2,3,4,5-tetramethoxy-6-methylphenyl)decyl)phosphonium 11

(10-(4,5-dimethoxy-2-methylphenyl)decyl)triphenylphosphonium 12

(10-(3-methyl-1,4-dioxo-1,4-dihydronaphthalen-2-yl)decyl)triphenylphosphonium 13

Triphenyl(10-phenyldecyl)phosphonium 14

(10-cyclohexyldecyl)triphenylphosphonium chloride 15

(10- (3, 4-dimethyl phenyl) decyl) triphenylphosphonium 16

10-(2,5-Dimethoxy-3,4-demethyl-phenyl)decyl-triphenyl-phosphonium 17

(10- (3, 4-dimethoxyphenyl) decyl) triphenylphosphonium 18

Triphenyl(8-(2,3,4,5-tetramethoxy-6-methylphenyl)octyl)phosphonium 19

(8-(4,5-dimethoxy-2-methylphenyl)octyl)triphenylphosphonium 20

Triphenyl(12-(2,3,4,5-tetramethoxy-6-methylphenyl)dodecyl)phosphonium 21

(12-(4,5-dimethoxy-2-methylphenyl)dodecyl)triphenylphosphonium 22

(10-(4,5-dimethoxy-2-methyl-3,6-dioxocyclohexadien-1-yl)decyl)triphenylphosphonium 23

2,2,5,5-Tetramethyl-3-(((10-(triphenylphosphonio)decyl)oxy)carbonyl)pyrrolidin-1-olate 24

(10-(4,5-dimethyl-3,6-dioxocyclohexa-1,4-dien-1-yl)decyl)triphenylphosphonium 25

(10-(6-hydroxy-2,5,7,8-tetramethylchroman-2-yl)decyl)triphenylphosphonium

II. Uses of Compounds of Formula 1

One. TRAP1 inhibition

One invention disclosed herein provides the use of the compound described above for inhibiting TRAP1.

The compounds disclosed herein reduce the expression of SDHB and SIRT3 by binding to TRAP1 and inhibiting the function of TRAP1. In addition, p-AMPK, which is additionally known as a marker for TRAP1 inhibition, CHOP (Control of tumor bioenergetics and survival stress signaling by mitochondrial HSP90s. Cancer cell 22, 331-344, Mitochondrial Hsp90s suppress calcium-mediated stress signals propagating from mitochondria to the ER in cancer cells. Molecular cancer 13, 148. Reference) increases (see Figs. 10, 12, 13, 15 and 17).

Unlike PU-H71, which is known to bind to the ATP-pocket-binding-site, the compound provided herein does not inhibit the activity of ATPase in a concentration-dependent manner in the case of TPP-alkyl. That is, the compounds provided herein are not related to ATPase activity inhibition. Additionally, the compounds provided herein do not bind to the ATP binding site. In one embodiment of the present invention, this was confirmed through ATP pocket binding force analysis. (See Figures 9, 11 and 14).

Accordingly, the compounds provided herein can bind to TRAP1 and inhibit TRAP1. More specifically, the compounds provided herein can inhibit TRAP1 without binding to the ATP binding site of TRAP1.

That is, the present specification may provide a TRAP1 inhibitor comprising a compound having the structure of Formula 1 or a pharmaceutically acceptable salt thereof. In this case, the compound or a pharmaceutically acceptable salt thereof may be characterized in that it does not bind to the ATP binding site.

In addition, in the present specification, a compound having a structure of Formula 1 or a pharmaceutically acceptable salt thereof may be used for preparing a TRAP1 inhibitor.

2. Inhibition of TRAP1 binding to client proteins

The compound can inhibit the activity of TRAP1 by binding to the TRAP1 stopping unit. More specifically, the compound binds to the TRAP1 client binding site and inhibits TRAP1 activity without binding to the ATP binding site.

To confirm this, the present inventors analyzed the binding structure of TRAP1 and MitoQ to prepare a fluorescent probe that binds to the client binding site, and then analyzed the binding ability of the compounds using this (Figs. 1, 2, 4, 6 and 8 Reference). Referring to the experimental results, it can be confirmed that all TPP-alkyl, antioxidant-TPP conjugates and other compounds bind to the TRAP1 Client binding site. Furthermore, as a result of ATPase activity analysis, confirmation of ATP binding site binding and inhibition of cytoplasmic Hsp90, it was confirmed that the compound of the present specification does not bind to the ATP binding site and inhibits the activity of TRAP1 (FIG. 9 , 11 and 14).

In addition, specifically, the compounds of the present specification are cytoplasmic Hsp90 client proteins (Akt, Cdk4), Hsp90 inhibition marker Hsp70 (Evidence for Efficacy of New Hsp90 Inhibitors Revealed by Ex Vivo Culture of Human Prostate Tumors. Clinical Cancer Research 18, 3562-3570.) and selectively inhibits only TRAP1 (see FIGS. 10, 12, 13, 15 and 17).

That is, the compound of the present specification binds to the stop unit of TRAP1 and does not affect the N-terminal site where ATP is bound to produce energy for the activity of hsp90s.

Accordingly, the compound provided herein can bind to the stopping unit of TRAP1 and inhibit the binding of a client protein known to bind to the stopping unit. That is, the compound of the present specification can be used to inhibit the binding of TRAP1 and a client protein.

In the present specification, a TRAP1-client protein binding inhibitor comprising a compound having the structure of Formula 1 or a pharmaceutically acceptable salt thereof may be provided.

In the present specification, a compound having the structure of Formula 1 or a pharmaceutically acceptable salt thereof may be used to prepare a TRAP1-client protein binding inhibitor.

3. pharmaceutical composition

A compound provided herein or a pharmaceutically acceptable salt thereof may be used as a pharmaceutical composition. That is, the present specification provides a pharmaceutical composition comprising the compound or a pharmaceutically acceptable salt thereof. In the present specification, the compound or a pharmaceutically acceptable salt thereof may be used for preparing a pharmaceutical composition.

The pharmaceutically acceptable salts include salts of compounds derived from various physiologically acceptable organic and inorganic counter ions. Such counter ions are well known in the art and include, for example, sodium, potassium, calcium, magnesium, aluminum, lithium and ammonium such as tetraalkylammonium and the like (when the molecule contains an acidic functional group); and when the molecule contains a basic functional group, salts of organic or inorganic acids such as hydrochloride, sulfate, phosphate, diphosphate, nitrate hydrobromide, tartrate, mesylate, acetate, malate, maleate, fumarate , tartrate, succinate, citrate, lactate, pamoate, salicylate, stearate, methanesulfonate, p-toluenesulfonate, oxalate, and the like. Suitable pharmaceutically acceptable salts thereof are described in Remington's Pharmaceutical Sciences, 17th Edition, pg. 1418 (1985) and P. Heinrich Stahl, Camille G. Wermuth (Eds.), Handbook of Pharmaceutical Salts Properties, Selection, and Use; 2002]. Examples of acid addition salts are acids such as hydroiodic acid, phosphoric acid, metaphosphoric acid, nitric acid and sulfuric acid, and organic acids such as alginic acid, ascorbic acid, anthranilic acid, benzoic acid, camphorsulfuric acid, citric acid, embonic acid (pamoic acid) ), ethanesulfonic acid, formic acid, fumaric acid, furoic acid, galacturonic acid, gentisic acid, gluconic acid, glucuronic acid, glutamic acid, glycolic acid, isonicotinic acid, isothionic acid, lanthanic acid, malic acid, mandelic acid, methanesulfonic acid , mucinic acid, pantothenic acid, phenylacetic acid, propionic acid, saccharic acid, salicylic acid, stearic acid, succinic acid, sulfinilic acid, trifluoroacetic acid and arylsulfonic acids such as benzenesulfonic acid and p-toluenesulfonic acid. . Examples of base addition salts formed with alkali metals and alkaline earth metals and organic bases are chloroprocaine, choline, N,N-dibenzylethylenediamine, diethanolamine, ethylenediamine, lysine, meglumine (N-methylglucamine) , and crocaine, as well as internally formed salts. Salts with non-physiologically acceptable anions or cations are within the scope of the present invention as useful intermediates for the preparation of physiologically acceptable salts and/or for use in non-therapeutic, eg in vitro, situations. there is. Pharmaceutically acceptable salts according to the present application include halogen salts, that is, fluorine salts, bromine salts, iodine salts and the like.

The pharmaceutical composition can be used to treat diseases that can be treated through inhibition of TRAP1.

The disease includes, in one embodiment, cancer. TRAP1 is known to be associated with cancer, and TRAP1 can be a target for cancer treatment (Regulation of Tumor Cell Mitochondrial Homeostasis by an Organelle-Specific Hsp90 Chaperone Network, 2007, Kang et al; Control of Tumor Bioenergetics and Survival Stress Signaling by Mitochondrial HSP90s, 2012, Chae et al; The mitochondrial chaperone TRAP1 as a candidate target of oncotherapy, 2001, Xie et al; TRAP1: a viable therapeutic target for future cancer treatments, 2017, Lettini et al.). In addition, referring to the experimental results, it can be confirmed that the compound provided herein inhibits TRAP1 in cancer cells and thereby reduces the size of cancer.

Accordingly, the compounds of the present specification can be used in the preparation of pharmaceutical compositions for cancer treatment.

The present specification may provide a pharmaceutical composition for treating cancer comprising a compound having the structure of Formula 1 or a pharmaceutically acceptable salt thereof.

In this case, the cancer may include thyroid cancer, stomach cancer, colon cancer, lung cancer, breast cancer, liver cancer, prostate cancer, pancreatic cancer, gallbladder cancer, biliary tract cancer, and the like. However, the disease is not limited to cancer, and includes all diseases known to be associated with TRAP1.

4. treatment method

The compound provided herein or a pharmaceutically acceptable salt thereof can be administered to a subject in need thereof and used to treat a specific disease. That is, the present specification provides a method for treating a specific disease comprising administering a pharmaceutical composition comprising the compound or a pharmaceutically acceptable salt thereof to a subject in need thereof.

These diseases include all diseases treatable through inhibition of TRAP1. The disease includes, in one embodiment, cancer, but is not limited thereto, and includes all diseases known to be associated with TRAP1. In this case, the cancer may include thyroid cancer, stomach cancer, colon cancer, lung cancer, breast cancer, liver cancer, prostate cancer, pancreatic cancer, gallbladder cancer, biliary tract cancer, and the like.

Administration of the pharmaceutical composition to a subject in need thereof may be performed through various routes. For example, orally (e.g., drenches such as aqueous or non-aqueous solutions or suspensions, pills, capsules (including sprinkle capsules and gelatin capsules), lumps, powders, granules, pastes for application to the tongue) ; absorption through the oral mucosa (eg sublingual); anal, rectal or vaginal (eg pessary, cream or foam); parenteral (intramuscularly, intravenously, subcutaneously or intrathecally, eg as a sterile solution or suspension); nasal cavity; intraperitoneal; subcutaneous; transdermal (eg, a patch applied to the skin); and topical (eg cream, ointment or spray applied to the skin, or eye drops) administration.

The subject is a mammal such as a human, or a non-human mammal. When administered to a subject, eg, a human, the composition or compound may be preferably administered, eg, as a pharmaceutical composition comprising a compound of the present application and a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers are those well known in the art and include, for example, aqueous solutions such as water or physiologically buffered saline or other solvents such as glycols, glycerol, oils such as olive oil, or injectable organic esters. or a carrier.

The actual dosage of the pharmaceutical composition may be varied in order to obtain an effective amount of the active ingredient to achieve the desired therapeutic response without being toxic to the patient for the particular patient, composition, and method of administration.

The selected dosage depends on the activity of the particular compound or combination of compounds employed, or its esters, salts or amides, the route of administration, the time of administration, the rate of excretion of the specific compound employed, the duration of treatment, and other drugs used in conjunction with the specific compound employed. , the compound and/or substance, age, sex, weight, condition, general health and medical history of the subject being treated, and other factors well known in the medical arts.

A physician or veterinarian skilled in the art can readily determine and prescribe the required therapeutically effective amount of the pharmaceutical composition. For example, a physician or veterinarian may start the dosage of the pharmaceutical composition or compound at a level lower than necessary to achieve the desired therapeutic effect and slowly increase the dosage until the desired effect is achieved. "Therapeutically effective amount" means a concentration of a compound sufficient to elicit the desired therapeutic effect.

It is generally understood that an effective amount of a compound may vary depending on the weight, sex, and medical history of the subject. Other factors that affect an effective amount include, but are not limited to, the severity of the subject's condition, the disorder being treated, the stability of the compound, and, if desired, other types of therapeutic agents administered in conjunction with the compounds of the present application. A total dosage can be delivered by administering multiple doses of the formulation. Methods for determining potency and dosage are known to those skilled in the art (Isselbacher et al. (1996) Harrison's Principles of Internal Medicine 13 ed., 1814-1882, incorporated herein by reference.

In certain embodiments, a compound provided herein may be administered alone or in combination with other types of therapeutic agents. As used herein, the term “conjoint administration” refers to any administration of two or more different therapeutic compounds such that a second compound is administered while the previously administered therapeutic compound is still effective in the body. (e.g., two compounds are simultaneously effective in a subject, and may include a synergistic effect of the two compounds). For example, different therapeutic compounds may be administered concomitantly or sequentially, either in the same formulation or in separate formulations. In certain embodiments, different therapeutic compounds may be administered within 1 hour, 12 hours, 24 hours, 36 hours, 48 hours, 72 hours or 1 week of each other. Thus, subjects undergoing such treatment can benefit from the combined effect of different therapeutic compounds.

In certain embodiments, co-administration of a compound provided herein with one or more additional therapeutic agents (e.g., one or more additional chemotherapeutic agents) provides an improvement over separate administration of a compound of the present application or one or more additional therapeutic agents separately. provide efficacy. In certain such embodiments, co-administration provides an additive effect, wherein an additive effect is the sum of the respective effects of separate administrations of a compound herein and one or more additional therapeutic agents.

III. Experimental Example

One. compound synthesis

The present inventors prepared SB-TM2, a fluorescent probe that binds to TRAP1, to confirm the binding ability of the compounds to TRAP1, and also synthesized and purchased compounds for use in experiments.

Methyltriphenylphosphonium Bromide (Alfa Aesar (Cat.# A15878)), Ethyltriphenylphosphonium Bromide (Alfa Aesar (Cat.# B23096)), Butyltriphenylphosphonium Bromide (Alfa Aesar (Cat.# A10504)) , Hexyltrephenylphosphonium bromide (Alfa Aesar (Cat.# A13826)), octyltriphenylphosphonium bromide (Alfa Aesar (Cat.# L02412)), decyltriphenylphosphonium bromide (Alfa Aesar (Cat.# A11021) )), dodecyltriphenylphosphonium bromide (Alfa Aesar (Cat.# A14295)), tetradecyltriphenylphosphonium bromide (Alfa Aesar (Cat.# L04311)), hexadecyltriphenylphosphonium bromide (Alfa Aesar (Cat.# A14295)) Cat.# A15180)), MitoQ (BioVision, Cat #: B1309), SkQ1 (MedchemExpress Cat. #: HY-100474) and Mito-TEMPO (SIGMA Cat #: SML0737) were purchased and used in the experiment.

1-1. SB-TM2 probe synthesis

SB-TM2: 6-(11-oxo-2,3,5,6,7,11-hexahydro-1H-pyrano[2,3-f]pyrido[3,2,1-ij]quinoline- 10-carboxamido)hexyl)triphenylphosphonium methane sulfonate.

Step A. N-(6-Hydroxyhexyl)-20-oxo-27-oxa-23-azatetracycloheptadeca-(14),1(16),15(18),17(19)-tetraene -16-carboxamide

Compound 14-oxo-20-oxa-16-azatetracycloheptadeca-(8),1(10),9(12),11(13)-tetraene-10-carboxylic acid in DMF (30 mL) (376.5 mg, 1.31 mmol), 6-aminohexan-1-ol (170.12 mg, 1.38 mmol) and HATU (602.15 mg, 1.58 mmol) was added DIPEA (511.67 mg, 3.95 mmol), followed by 25 °C C for 12 hours. LCMS showed that the starting material was consumed and the desired product was formed. The reaction mixture was poured into 60 mL of H ₂ O, then extracted with ethyl acetate (50 mL x 2). The combined ether acetate was washed with saturated brine (50 mL x 2) and dried over Na ₂ SO ₄ . The organic layer was evaporated to dryness to give the crude product as a yellow foam which was purified by column chromatography (SiO2, Petroleum ether/ EtOAc = 1:0 to 1:4) to give the desired product as a yellow foam (465 mg, 1.17 mmol, 92.4% yield) was obtained.

MS (ESI): mass calcd. for C ₂₂ H ₂₈ N ₂ O ₄ , 384.2; m/z found, 385.2 [M+H]+.

1H NMR (400MHz, CDCl3) δ 1.36 - 1.50 (m, 4 H), 1.51 - 1.72 (m, 4 H), 1.92 - 2.06 (m, 4 H), 2.78 (t, J=6.0 Hz, 2 H) , 2.90 (t, J=6.3 Hz, 2 H), 3.28 - 3.39 (m, 4 H), 3.45 (q, J=6.8 Hz, 2 H), 3.65 (t, J=6.5 Hz, 2 H), 7.02 (s, 1 H), 8.61 (s, 1 H), 8.88 (br s, 1 H).

Step B. 6-[(21-oxo-29-oxa-24-azatetracycloheptadeca-1(15),2(17),16(19),18(20)-tetraene-17-carbonyl )amino]hexyl methanesulfonate

N-(6-hydroxyhexyl)-20-oxo-27-oxa-23-azatetracycloheptadeca-(14),1(16),15(18),17(19)-tetraene-16- Carboxamide (400 mg, 1.04 mmol) was dissolved in DCM (25 mL) then DMAP (1.27 mg, 10.40 umol) and DIEA (1.34 g, 10.40 mmol) were added and the resulting mixture cooled to 0 °C. . MsCl (106 mg, 9.25 mmol) was added dropwise and then stirred at 0 °C for 2 h. TLC (petroleum ether : EtOAc = 1:1, Rf = 0.43) showed that the starting alcohol was consumed and one major new spot was formed. The mixture was diluted with dichloromethane (35 mL) and sat. aq. NaHCO ₃ (35 mL). The organic layer was collected, dried over Na ₂ SO ₄ , filtered and concentrated in vacuo to give the crude product as a yellow solid. HNMR showed it to be sufficiently pure for the next step reaction.

1H NMR (400MHz, CDCl3) δ 1.36 - 1.56 (m, 4 H), 1.60 - 1.85 (m, 4 H), 1.99 (m, 4 H), 2.78 (m, 2 H), 2.90 (m, 2 H) ), 3.01 (s, 3 H), 3.26 - 3.39 (m, 4 H), 3.44 (q, J=6.6 Hz, 2 H), 4.23 (t, J=6.5 Hz, 2 H), 7.02 (s, 1 H), 8.61 (s, 1 H), 8.88 (br d, J=4.9 Hz, 1 H).

Step C. (6-(11-oxo-2,3,5,6,7,11-hexahydro-1H-pyrano[2,3-f]pyrido[3,2,1-ij]quinoline- 10-carboxamido)hexyl)triphenylphosphonium methane sulfonate

6-[(21-oxo-29-oxa-24-azatetracycloheptadeca-1(15),2(17),16(19),18(20)-tetraene-17-carbonyl)amino] Hexyl methane sulfonate (97 mg, 203.41 umol) was thoroughly mixed with triphenylphosphane (266.76 mg, 1.02 mmol) in 5 mL of toluene (5 mL), and then the resulting solution was refrigerated at 130 °C under N ₂ for 18 stirred for an hour. LCMS showed that the sulfonate was consumed and the desired product was produced as the major constituent. The reaction mixture was cooled to room temperature and evaporated to dryness. TLC (DCM : MeOH = 10:1, Rf = 0.24) showed the formation of one major new spot under OPPh ₃ . The crude product was purified by flash column chromatography on silica gel, eluting first with 50-100% EtOAc in petroleum over 20 min and holding at 100% for 15 min to remove all impurities above the desired product, followed by 0 min over 20 min. -10% MeOH in DCM and held at 10% for 20 min) to give the desired product as triphenylphosphonium methanesulfonate salt. The product was further lyophilized to remove residual solvent to obtain a yellow solid (106 mg, 145.55 umol, 23.85% yield, 98.24% purity).

MS (ESI): mass calcd. for C40H42N2O3P+, 629.29; m/z found, 629.5 [M+H]+.

1H NMR (400MHz, MeOD) δ 1.45 (m, 2 H), 1.52 - 1.78 (m, 6 H), 1.90 - 2.04 (m, 4 H), 2.69 (s, 3 H), 2.74 - 2.87 (m, 4 H), 3.34 - 3.50 (m, 8 H), 7.08 (s, 1 H), 7.62 - 7.97 (m, 15 H), 8.44 (s, 1 H), 9.04 (br s, 1 H); 31P NMR (162 MHz, METHANOL-d4) δ ppm 23.81 (s, 1 P).

1-2. Synthesis of (10-(3-bromo-4,5,6-trimethoxy-2-methylphenyl)decyl)triphenylphosphonium bromide

Step A. 10-Bromo-1-(2-hydroxy-3,4-dimethoxy-6-methyl-phenyl)decan-1-one

Freshly powdered AlCl ₃ (457.89 mg, 3.43 mmol) was added to 10-bromodecanoyl chloride (0.536 g, 1.89 mmol) and 1,2,3-trimethoxy-5-methyl-benzene in dry DCE (10 mL). (312.86 mg, 1.72 mmol) and stirred at 25° C. for 40 hr. LCMS showed that the desired product was formed with major components. The mixture was poured into ice water and extracted with CH ₂ Cl ₂ (50mL*2). The combined extracts were washed with water, dried over Na ₂ SO ₄ and concentrated to give an oil which was purified by column chromatography (SiO2, 10:0 to 10:1 Petroleum ether/EtOAc) to obtain a colorless oil (520 mg, 66.56 % yield) was obtained.

MS (ESI): mass calcd. for C ₁₉ H ₂₉ BrO ₄ , 400.12; m/z found, 402.8 [M+H]+.

1H NMR (400MHz, CDCl3) δ 1.21 - 1.55 (m, 10 H), 1.56 - 1.78 (m, 2 H), 1.85 (m, 2 H), 2.46 (s, 3 H), 2.89 (t, J= 7.4 Hz, 2 H), 3.41 (t, J=6.8 Hz, 2 H), 3.88 (d, J=12.3 Hz, 6 H), 6.31 (s, 1 H), 10.38 (s, 1 H).

Step B. 2-(10-Bromodecyl)-5,6-dimethoxy-3-methyl-phenol

10-Bromo-1-(2-hydroxy-3,4-dimethoxy-6-methyl-phenyl)decan-1-one (520 mg, 1.14 mmol) was dissolved in TFA (10 mL) followed by EtSiH (2 mL) was added, followed by stirring at 80 °C for 12 h. LCMS showed that the starting ketone was consumed and a new peak formed. The reaction mixture was evaporated to dryness and purified by column chromatography (SiO2, 5:0 to 5:1 Petroleum ether/EtOAc) to give a colorless oil (410 mg, 82.34% yield).

MS (ESI): mass calcd. for C ₁₉ H ₃₁ BrO ₃ , 386.15; m/z found, 388.9 [M+H]+.

1H NMR (400MHz, CDCl3) δ 1.22 - 1.55 (m, 14 H), 1.86 (quin, J=7.1 Hz, 2 H), 2.26 (s, 3 H), 2.51 - 2.65 (m, 2 H), 3.42 (t, J=6.9 Hz, 2 H), 3.86 (m, 6 H), 5.82 (s, 1 H), 6.29 (s, 1 H).

Step C. 4-Bromo-2-(10-bromodecyl)-5,6-dimethoxy-3-methyl-phenol

2-(10-Bromodecyl)-5,6-dimethoxy-3-methyl-phenol (410 mg, 940.98 umol) and NaBr (145.23 mg, 1.41 mmol) were dissolved in AcOH (10 mL), followed by H ₂ O ₂ (160.04 mg, 1.41 mmol, 30%) was added at 25°C and then stirred for 2 hours. LCMS showed that the starting material was consumed and a new peak formed. The reaction mixture was quenched with water (50 mL) and extracted with EtOAc (40 mL x 2). The combined organic layers were washed with saturated NaHCO ₃ (40 mL) until pH > 7, then dried over Na ₂ SO ₄ and concentrated to a colorless oil (300 mg, crude).

MS (ESI): mass calcd. for C ₁₉ H ₃₀ Br ₂ O ₃ , 464.06; m/z found, 466.9 [M+H]+.

1H NMR (400MHz, CDCl3) δ 1.22 - 1.55 (m, 14 H), 1.86 (quin, J=7.1 Hz, 2 H), 2.26 (s, 3 H), 2.51 - 2.65 (m, 2 H), 3.42 (t, J=6.9 Hz, 2 H), 3.85 (s, 3 H), 3.93 (s, 3 H), 5.77 (s, 1 H).

Step D. (10-(3-bromo-4,5,6-trimethoxy-2-methylphenyl)decyl)triphenylphosphonium bromide

4-Bromo-2-(10-bromodecyl)-5,6-dimethoxy-3-methyl-phenol (300 mg, 597.11 umol) and PPh ₃ (939.68 mg, 3.58 mmol) in toluene (1 mL) dissolved, then stirred at 130°C under N ₂ for 18 hours. TLC (DCM: MeOH = 10:1, Rf = 0.2) showed that one main peak was formed below OPPh ₃ . The reaction mixture was evaporated to give a brown residue, which was analyzed by Prep-HPLC (Column: 3_Phenomenex Luna C18 75*30mm*3um; mobile phase: [water(0.2%FA)-ACN]; B%: 52%-82%, 6 min). The desired product was obtained as a white solid after lyophilization (16 mg, 12.24% yield, 97.2% purity).

MS (ESI): mass calcd. for C ₃₇ H ₄₅ BrO ₃ P+, 647.23; m/z found, 649.3 [M+H]+.

1H NMR (400 MHz, CHLOROFORM-d) δ 1.13 - 1.70 (m, 16 H), 2.34 (s, 3 H), 2.56 - 2.76 (m, 2 H), 3.65 - 3.79 (m, 2 H), 3.68 - 3.77 (m, 1 H), 3.83 (s, 3 H), 3.88 (s, 3 H), 7.61 - 7.93 (m, 15 H), 8.76 (s, 1 H); 31P NMR (162 MHz, CHLOROFORM-d) δ 24.47 (s, 1 P).

1-3. Synthesis of (10-(2-bromo-5-hydroxy-3,4-dimethoxy-6-methylphenyl)decyl)triphenylphosphonium formate

Step A. 5-(10-Bromodecyl)-1,2,3-trimethoxy-benzene

To a solution of 5-bromo-1,2,3-trimethoxy-benzene (1.3 g, 5.26 mmol, 1 eq) in THF (20 mL) was n-BuLi (2.5 M, 2.10 mL, 1 eq) - It was added dropwise at 78 °C. After addition, the mixture was stirred at that temperature for 1 hour, then a solution of 1,10-dibromodecane (3.16 g, 10.52 mmol, 2 eq) in THF (10 mL) was added dropwise at -78 °C. The resulting mixture was stirred at 20 °C for 11 hours. LCMS showed that 50.6% of the desired mass was detected. The residue was diluted with sat.NH ₄ Cl (10 mL) and extracted with EtOAc (50 mL * 3). The combined organic layers were dried over Na2SO4 and concentrated by filtration under reduced pressure to give a residue. The residue was purified by column chromatography (SiO2, Petroleum ether/Ethyl acetate=100/0 to 95/5). Compound 5-(10-bromodecyl)-1,2,3-trimethoxy-benzene (580 mg, 1.02 mmol, 19.35% yield, 68% purity) was obtained as a colorless oil.

MS (ESI): mass calcd. for C ₁₉ H ₃₁ BrO ₃ , 387.4; m/z found, 387.1 [M+H]+.

1H NMR (400MHz, CDCl3) δ = 6.40 (s, 2H), 3.86 (s, 6H), 3.83 (s, 3H), 3.42 (t, J=6.8 Hz, 2H), 2.59 - 2.52 (m, 2H) , 1.86 (quin, J=7.2 Hz, 2H), 1.60 (br d, J=5.5 Hz, 2H), 1.48 - 1.38 (m, 2H), 1.38 - 1.26 (m, 10H)

Step B. 6-(10-Bromodecyl)-2,3,4-trimethoxy-benzaldehyde

A solution of 5-(10-bromodecyl)-1,2,3-trimethoxy-benzene (580 mg, 1.02 mmol, 1 eq) in dry CH ₂ Cl ₂ (2 mL) was dissolved in AlCl ₃ (239 mg, 1.79 mmol, 97.95 uL, 1.76 eq) of dry CH2Cl2 (8 mL) was added gradually at 0°C dropwise. The mixture was stirred at the same temperature for 45 min, and a dry CH ₂ Cl ₂ (2 mL) solution of dichloro (methoxy)methane (188.97 mg, 1.64 mmol, 145.36 uL, 1.61 eq, 68% purity) was added thereto over 10 min. It was added dropwise gradually over The mixture was stirred at 0 °C for 2 h 5 min. LCMS showed the reaction to be complete. The reaction mixture was poured into 30 mL of ice water, the methylene chloride phase was separated and the aqueous phase was extracted twice with 50 mL of methylene chloride. The combined organic layers were dried over Na ₂ SO ₄ and concentrated by filtration under reduced pressure to give a residue. The crude product was used in the next step without further purification. Compound 6-(10-bromodecyl)-2,3,4-trimethoxy-benzaldehyde (510 mg, 858.27 umol, 84.29% yield, 69.9% purity) was obtained as a colorless oil.

MS (ESI): mass calcd. for C ₂₀ H ₃₁ BrO ₄ ,415.4; m/z found, 415.1 [M+H]+.

1H NMR (400MHz, CDCl3) δ = 10.41 (s, 1H), 6.53 (s, 1H), 4.00 (s, 3H), 3.95 (s, 3H), 3.89 (s, 3H), 3.43 (t, J= 6.9 Hz, 2H), 2.99 - 2.92 (m, 2H), 1.93 - 1.82 (m, 2H), 1.49 - 1.39 (m, 4H), 1.32 (br s, 10H)

Step C. 6-(10-Bromodecyl)-2-hydroxy-3,4-dimethoxy-benzaldehyde

BCl3 (1 M, 1.9 mL, 2.21 eq) was converted to 6-(10-bromodecyl)-2,3,4-trimethoxy-benzaldehyde (510.00 mg, 858.27 umol, 1 eq in CH ₂ Cl ₂ (10 mL)). , 69.9% purity) at 0 °C. The mixture was stirred at 0 °C for 30 min and then at 20 °C for 30 min. LCMS showed the reaction to be complete. The residue was poured into ice water (30 mL) and extracted with CH ₂ Cl ₂ (50 mL * 3). The combined organic layers were dried over Na ₂ SO ₄ and concentrated by filtration under reduced pressure to give a residue. The residue was purified by column chromatography (SiO ₂ , Petroleum ether/Ethyl acetate=100/0 to 95/5). Compound 6-(10-bromodecyl)-2-hydroxy-3,4-dimethoxy-benzaldehyde (300 mg, 583.06 umol, 67.93% yield, 78% purity) was obtained as a colorless oil.

MS (ESI): mass calcd. for C ₁₉ H ₂₉ BrO ₄ ,401.3; m/z found, 401.1 [M+H]+.

1H NMR (400MHz, CDCl3) δ = 12.30 - 12.20 (m, 1H), 10.24 - 10.03 (m, 1H), 6.34 (s, 1H), 3.96 (s, 3H), 3.89 (s, 3H), 3.43 ( t, J=6.9 Hz, 2H), 2.90 - 2.83 (m, 2H), 1.88 (quin, J=7.1 Hz, 2H), 1.70 - 1.60 (m, 2H), 1.50 - 1.38 (m, 3H), 1.49 - 1.29 (m, 1H)

Step D. 3-Bromo-2-(10-bromodecyl)-6-hydroxy-4,5-dimethoxy-benzaldehyde

To a solution of 6-(10-bromodecyl)-2-hydroxy-3,4-dimethoxy-benzaldehyde (250 mg, 622.92 umol, 1 eq) in CHCl ₃ (2.5 mL) and CCl ₄ (2.5 mL) NBS (133.04 mg, 747.51 umol, 1.2 eq) was added at 0°C. The mixture was stirred at 0 °C for 1 hour. The mixture was then stirred at 20°C for 11 hours. LCMS showed the reaction to be complete. The mixture sat. It was diluted with NaHCO ₃ (10 mL) and extracted with EtOAc (20 mL * 3). The combined organic layers were dried over Na ₂ SO ₄ and concentrated by filtration under reduced pressure to give a residue. The residue was purified by prep-TLC (SiO2, Petroleum ether/Ethyl acetate= 4:1). Compound 3-bromo-2-(10-bromodecyl)-6-hydroxy-4,5-dimethoxy-benzaldehyde (200 mg, 307.77 umol, 49.41% yield, 73.9% purity) was obtained as a yellow oil.

MS (ESI): mass calcd. for C ₁₉ H ₂₈ Br ₂ O ₄ , 480.2; m/z found, 481.0 [M+H]+.

Step E. 5-(10-Bromodecyl)-2,3-dimethoxy-6-methyl-phenol

3-Bromo-2-(10-bromodecyl)-6-hydroxy-4,5-dimethoxy-benzaldehyde (190 mg, 292.38 umol, 1 eq, 73.9% purity in CH ₂ Cl ₂ (4 mL) ) and Et ₃ SiH (169.99 mg, 1.46 mmol, 233.50 uL, 5 eq) at 0 °C was added TFA (708.40 mg, 6.21 mmol, 460 uL, 21.25 eq) dropwise over 5 min through an addition funnel. . The reaction mixture was stirred at 0 °C for 2 hours. LCMS showed the reaction to be complete. Sat the mixture slowly. Poured into NaHCO3 (50 mL) and extracted with 100 mL of CH ₂ Cl ₂ (100 mL * 3). The combined organic layers were dried over Na ₂ SO ₄ and concentrated by filtration under reduced pressure to give a residue. The residue was purified by prep-TLC (SiO2, Petroleum ether: Ethyl acetate=4:1). Compound 5-(10-bromodecyl)-2,3-dimethoxy-6-methyl-phenol (130 mg, 241.64 umol, 82.65% yield, 72% purity) was obtained as a colorless oil.

Step F. 4-Bromo-5-(10-bromodecyl)-2,3-dimethoxy-6-methyl-phenol

5-(10-bromodecyl)-2,3-dimethoxy-6-methyl-phenol (130 mg, 241.64 umol, 1 eq, 72% purity) and NaBr (37.29 mg, 362.46 umol) in AcOH (5 mL) , 11.65 uL, 1.5 eq) was added H2O2 (41.09 mg, 362.46 umol, 34.82 uL, 30% purity, 1.5 eq) and then stirred at 20 °C for 3 h. LCMS showed the reaction to be complete. The residue was diluted with sat NaHCO ₃ : Na ₂ S ₂ O ₃ =10:1 (30) mL and extracted with EtOAc (30 mL * 3). The combined organic layers were washed with brine (10 mL), dried over Na ₂ SO ₄ and concentrated by filtration under reduced pressure to give a residue. The crude product was used in the next step without further purification. Compound 4-bromo-5-(10-bromodecyl)-2,3-dimethoxy-6-methyl-phenol (140 mg, 195.18 umol, 80.77% yield, 65% purity) was obtained as a yellow oil.

MS (ESI): mass calcd. for C ₁₉ H ₃₀ Br ₂ O ₃ , 466.3; m/z found, 466.9 [M+H]+.

1H NMR (400MHz, CDCl3) δ = 5.73 (s, 1H), 3.86 (s, 3H), 3.78 (s, 3H), 3.34 (t, J=6.9 Hz, 2H), 2.71 - 2.64 (m, 2H) , 2.14 (s, 3H), 1.84 - 1.76 (m, 2H), 1.37 (br d, J=4.1 Hz, 7H), 1.24 (br s, 7H)

Step G. (10-(2-Bromo-5-hydroxy-3,4-dimethoxy-6-methylphenyl)decyl)triphenylphosphonium formate

4-Bromo-5-(10-bromodecyl)-2,3-dimethoxy-6-methyl-phenol (140 mg, 195.18 umol, 1 eq, 65% purity) and PPh ₃ in toluene (2 mL) (255.96 mg, 975.88 umol, 5 eq) was heated at 125 °C under N ₂ for 8 h. LCMS showed the reaction to be complete. The solvent was removed under vacuum to give a residue. The residue was purified by column chromatography (SiO2, Petroleum ether/Ethyl acetate=100/0 to 0/100; Ethyl acetate: MeOH=100/0 to 92/8). The residue was purified by prep-HPLC (FA condition; column: Xtimate C18 100*30mm*3um; mobile phase: [water(0.225%FA)-ACN]; B%: 40%-70%, 8min). The compound (10-(2-bromo-5-hydroxy-3,4-dimethoxy-6-methylphenyl)decyl)triphenylphosphonium formate (6 mg, 8.61 umol, 4.41% yield, 99.54% purity) Obtained as a colorless gum.

MS (ESI): mass calcd. for C ₃₇ H ₄₅ BrO ₃ P+, 648.6; m/z found, 649.2 [M+H]+.

1H NMR (400MHz, CDCl ₃ ) δ = 8.56 (br s, 1.309H), 7.78 - 7.59 (m, 15H), 3.84 (s, 3H), 3.76 (s, 3H), 3.44 (br s, 2H), 2.70 - 2.59 (m, 2H), 2.13 (s, 3H), 1.50 (br s, 4H), 1.40 - 1.12 (m, 12H)

31P NMR (162MHz, CDCl3) δ = 24.17 (s, 1P)

1-4. Synthesis of (10-(3-bromo-4,5,6-trimethoxy-2-methylphenyl)decyl)triphenylphosphonium bromide

Step A. 10-Bromo-1-(2,3,4-trimethoxy-6-methyl-phenyl)decan-1-one

Stirring of 4-bromo-1,2,3-trimethoxy-5-methyl-benzene (449.11 mg, 1.72 mmol) and 10-bromodecanoyl chloride (536.94 mg, 1.89 mmol) in DCE (10 mL) AlCl3 (206.41 mg, 1.55 mmol) was added to the solution and then stirred at 25 °C for 18 h. LCMS showed that the desired product was formed as a major constituent. TLC (Petroleum ether : EtOAc = 3:1, Rf = 0.4) showed that one major new spot was formed. The reaction mixture was poured into ice water and extracted with DCM (30 mL x3), then dried over Na ₂ SO ₄ and concentrated to give a yellow oil which was purified by flash column on silica gel (0-100% EtOAc in petroleum). ether over 30 min) a colorless oil was obtained (215 mg, 29.1% yield).

MS (ESI): mass calcd. for C ₂₀ H ₃₁ BrO ₄ , 414.14; m/z found, 416.8 [M+H]+.

1H NMR (400MHz, CDCl3) δ 1.32 (m, 8 H), 1.38 - 1.49 (m, 2 H), 1.67 (m, 2 H), 1.86 (quin, J=7.2 Hz, 2 H), 2.19 (s , 3 H), 2.75 (t, J=7.4 Hz, 2 H), 3.41 (t, J=6.9 Hz, 2 H), 3.77 - 3.92 (m, 9 H), 6.48 (s, 1 H).

Step B. 4-(10-Bromodecyl)-1,2,3-trimethoxy-5-methyl-benzene

To a stirred solution of 10-bromo-1-(2,3,4-trimethoxy-6-methyl-phenyl)decan-1-one (210 mg, 455.03 umol) in TFA (10 mL) was added Et ₃ SiH (1.46 g, 12.52 mmol, 2 mL) was added at 25 °C, followed by stirring at 80 °C for 2 h. LCMS showed that the desired product was formed as a major constituent. TLC (petroleum ether: EtOAc = 4:1, Rf =0.45) showed that one major new spot was formed. The reaction mixture was evaporated to dryness in vacuo to give a colorless oil which was further purified by flash column chromatography on silica gel (25 g, 0-50% EtOAc in petroleum ether over 30 min). The desired product 4-(10-bromodecyl)-1,2,3-trimethoxy-5-methyl-benzene (118 mg, 250.93 umol, 55.15% yield) was obtained as a colorless oil.

MS (ESI): mass calcd. for C20H33BrO3, 400.16; m/z found, 403.0 [M+H]+.

1H NMR (400MHz, CDCl3) δ 1.20 - 1.54 (m, 14 H), 1.77 - 1.96 (m, 2 H), 2.27 (s, 3 H), 2.46 - 2.64 (m, 2 H), 3.42 (t, J=6.8 Hz, 2 H), 3.76 - 3.97 (m, 9 H), 6.49 (s, 1 H).

Step C. 1-Bromo-5-(10-bromodecyl)-2,3,4-trimethoxy-6-methyl-benzene

A stirred mixture of 4-(10-bromodecyl)-1,2,3-trimethoxy-5-methyl-benzene (118 mg, 250.93 umol) and NaBr (38.73 mg, 376.39 umol) in AcOH (5 mL) To the solution was added H ₂ O ₂ (42.68 mg, 376.39 umol) and then stirred at 25 °C for 2 h. LCMS showed that the desired product was formed as a major constituent. The reaction mixture was partitioned between EtOAc/H ₂ O (80 mL/60 mL). Sat the organic layer to pH >7. aq. NaHCO ₃ (60 mL). The collected organic layers were dried over Na ₂ SO ₄ and concentrated to give a yellow oil (140 mg, crude). HNMR showed that the desired product was consistent with sufficient purity for the next step.

MS (ESI): mass calcd. for C ₂₀ H ₃₂ Br ₂ O ₃ , 478.07; m/z found, 481.0 [M+H]+.

1H NMR (400MHz, CDCl3) δ 1.20 - 1.52 (m, 14 H), 1.78 - 1.94 (m, 2 H), 2.36 (s, 3 H), 2.62 (m, 2 H), 3.42 (t, J= 6.9 Hz, 2 H), 3.81 - 3.98 (m, 9 H).

Step D. (10-(3-bromo-4,5,6-trimethoxy-2-methylphenyl)decyl)triphenylphosphonium bromide

1-bromo-5-(10-bromodecyl)-2,3,4-trimethoxy-6-methyl-benzene (140 mg, 279.13 umol) and PPh ₃ (366.06 mg, 1.40 mmol) was heated at 130 °C under N ₂ for 18 h. LCMS showed the desired product was formed. TLC (DCM: MeOH = 10:1, Rf = 0.2) showed the formation of a major new peak under OPPh ₃ . Evaporation of the reaction mixture gave a brown residue which was purified by flash column chromatography on silica gel (25 g, 0-15% MeOH in DCM over 30 min). The desired product was obtained as a white solid after lyophilization (108.5 mg, 51.41% yield, 98.2% purity).

MS (ESI): mass calcd. for C ₃₈ H ₄₇ BrO ₃ P+, 661.24; m/z found, 663.3 [M+H]+.

1H NMR (400 MHz, CHLOROFORM-d) δ 1.12 - 1.50 (m, 12 H), 1.64 (m, 4 H), 2.34 (s, 3 H), 2.52 - 2.71 (m, 2 H), 3.77 - 3.97 (m, 11 H), 7.60 - 7.97 (m, 15 H); 31P NMR (162 MHz, CHLOROFORM-d) δ 24.53 (s, 1 P).

1-5. Synthesis of (10-(2-bromo-3,4,5-trimethoxy-6-methylphenyl)decyl)triphenylphosphonium bromide

Step A. 5-(10-Bromodecyl)-1,2,3-trimethoxy-benzene

To a solution of 5-bromo-1,2,3-trimethoxy-benzene (2 g, 8.09 mmol, 1 eq) in THF (30 mL) was added n-BuLi (2.5 M, 3.24 mL, 1 eq) - It was added dropwise at 78 °C. After addition, the mixture was stirred at that temperature for 1 hour, then a solution of 1,10-dibromodecane (4.86 g, 16.19 mmol, 2 eq) in THF (10 mL) was added dropwise at -78 °C. The resulting mixture was stirred at 20 °C for 11 h. LCMS showed that 20% of the desired mass was detected. The residue was diluted with sat.NHCl (10 mL) and extracted with EtOAc (50 mL * 3). The combined organic layers were dried over Na2SO4, filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO2, Petroleum ether/Ethyl acetate=100/0 to 95/5). Compound 5-(10-bromodecyl)-1,2,3-trimethoxy-benzene (430 mg, 395.86 umol, 4.89% yield, 35.66% purity) was obtained as a colorless oil.

MS (ESI): mass calcd. for C19H31BrO3, 387.4; m/z found, 389.1 [M+H]+.

Step B. 6-(10-Bromodecyl)-2,3,4-trimethoxy-benzaldehyde

_{AlCl 3 (} 178 mg, 1.33 mmol, 72.95 uL, 3.37 eq) of dry CH ₂ Cl ₂ (6 mL) solution was added dropwise at 0°C. The mixture was stirred at the same temperature for 45 min and a solution of dry CH ₂ Cl ₂ (2 mL) of dichloro(methoxy)methane (140 mg, 1.22 mmol, 107.69 uL, 3.08 eq) was added dropwise over 10 min. . The mixture was stirred at 0 °C for 2 h 5 min. LCMS showed the reaction to be complete. The reaction mixture was poured into 30 mL of ice water, the methylene chloride phase was separated, and the aqueous phase was extracted twice with 50 mL of methylene chloride. The combined organic layers were dried over Na ₂ SO ₄ , filtered and concentrated under reduced pressure to give a residue. The crude product was used in the next step without further purification. Compound 6-(10-bromodecyl)-2,3,4-trimethoxy-benzaldehyde (410 mg, 384.97 umol, 97.25% yield, 39% purity) was obtained as a colorless oil.

MS (ESI): mass calcd. for C ₂₀ H ₃₁ BrO ₄ , 415.4; m/z found, 415.2 [M+H]+.

Step C. 1-(10-Bromodecyl)-3,4,5-trimethoxy-2-methyl-benzene

6-(10-bromodecyl)-2,3,4-trimethoxy-benzaldehyde (410 mg, 384.97 umol, 1 eq, 39% purity) and Et ₃ SiH (447.64 mg, 3.85 mmol, 614.89 uL, 10 To the mixture of eq) was added TFA (3 mL). The mixture was stirred at 20 °C for 12 hours. LCMS showed the reaction to be complete. Sat the mixture slowly. Poured into NaHCO3 (50 mL) and extracted with CH2Cl2 (50 mL*3). The combined organic layers were dried over Na ₂ SO ₄ , filtered and concentrated under reduced pressure to give a residue. The residue was purified by prep-TLC (SiO2, Petroleum ether/Ethyl acetate= 4:1). Compound 1-(10-bromodecyl)-3,4,5-trimethoxy-2-methyl-benzene (120 mg, 152.18 umol, 39.53% yield, 50.9% purity) was obtained as a colorless oil.

MS (ESI): mass calcd. for C20H33BrO3, 401.4; m/z found, 402.8 [M+H]+.

Step D. 1-Bromo-2-(10-bromodecyl)-4,5,6-trimethoxy-3-methyl-benzene

1-(10-bromodecyl)-3,4,5-trimethoxy-2-methyl-benzene (120 mg, 152.18 umol, 1 eq, 50.9% purity) and NaBr (15.66 mg) in AcOH (4 mL) , 152.18 umol, 4.89 uL, 1 eq) was added H2O2 (17.25 mg, 152.18 umol, 14.62 uL, 30% purity, 1 eq) and then stirred at 20 °C for 12 h. LCMS showed the reaction to be complete. The residue was diluted with sat NaHCO ₃ : Na ₂ S ₂ O ₃ =10:1 (30) mL and extracted with EtOAc (30 mL * 3). The combined organic layers were washed with brine (10 mL), dried over Na ₂ SO ₄ , filtered and concentrated under reduced pressure to give a residue. The crude product was used in the next step without further purification. Compound 1-bromo-2-(10-bromodecyl)-4,5,6-trimethoxy-3-methyl-benzene (130 mg, crude) was obtained as a yellow oil.

MS (ESI): mass calcd. for C ₂₀ H ₃₂ Br ₂ O ₃ , 480.3; m/z found, 480.9 [M+H]+.

Step E. 1-Bromo-2-(10-BLAHdecyl)-4,5,6-trimethoxy-3-methyl-benzene

1-Bromo-2-(10-bromodecyl)-4,5,6-trimethoxy-3-methyl-benzene (130 mg, 162.41 umol, 1 eq, 60% purity) in toluene (2 mL) and PPh ₃ (212.99 mg, 812.04 umol, 5 eq) was heated at 125 °C under N ₂ for 12 h. LCMS showed the reaction to be complete. The solvent was removed in vacuo to give a residue. The residue was purified by column chromatography (SiO2, Petroleum ether/Ethyl acetate=100/0 to 0/100; Ethyl acetate:MeOH=100/0 to 92/8). Compound 1-bromo-2-(10-BLAHdecyl)-4,5,6-trimethoxy-3-methyl-benzene (30 mg, 39.69 umol, 24.44% yield, 98.234% purity) was obtained as a colorless oil. .

MS (ESI): mass calcd. for C ₃₈ H ₄₇ BrO ₃ P+, 662.7; m/z found, 663.2 [M+H]+.

1H NMR (400MHz, CDCl ₃ ) δ = 7.93 - 7.66 (m, 15H), 3.94 - 3.78 (m, 11H), 2.78 - 2.67 (m, 2H), 2.22 (s, 3H), 1.64 (br s, 4H) ), 1.50 - 1.34 (m, 4H), 1.25 (br d, J=10.1 Hz, 8H) 31P NMR (162MHz, CDCl3) δ = 24.54 (s, 1P)

1-6. Synthesis of formic acid, triphenyl(10-(2,3,4,5-tetramethoxy-6-methylphenyl)decyl)phosphonium salt

Step A. 10-Bromo-1-(2,3,4,5-tetramethoxy-6-methyl-phenyl)decan-1-one

1,2,3,4-tetramethoxy-5-methyl-benzene (710 mg, 3.35 mmol, 1 eq) and 10-bromodecanoyl chloride (992.09 mg, 3.68 mmol, 1.1 eq) in DCE (15 mL) ) was added AlCl ₃ (401.45 mg, 3.01 mmol, 164.53 uL, 0.9 eq) and then stirred at 25 °C for 18 h. LCMS showed that the starting material was completely consumed. The reaction mixture was extracted with 10ml H ₂ O with DCM (15ml*3). The combined organic layers were then evaporated to dryness to give the product. The residue was purified by flash silica gel chromatography (ISCO®; 20 g SepaFlash® Silica Flash Column, Eluent of 15-20% Ethyl acetate/Petroleum ethergradient @ 45 mL/min). Compound 10-bromo-1-(2,3,4,5-tetramethoxy-6-methyl-phenyl)decan-1-one (800 mg, crude) was obtained as a yellow oil.

MS (ESI): mass calcd. for C ₂₁ H ₃₃ BrO ₅ , 444.15; m/z found, 445.2 [M+H]+.

Step B. 1-(10-Bromodecyl)-2,3,4,5-tetramethoxy-6-methyl-benzene

Stirred solution of 10-bromo-1-(2,3,4,5-tetramethoxy-6-methyl-phenyl)decan-1-one (350 mg, 785.83 umol, 1 eq) in TFA (15 mL) To the solution was added triethylsilane (2.55 g, 21.91 mmol, 3.50 mL, 27.89 eq) at 25 °C, followed by stirring at 80 °C for 2 h. LCMS showed that the starting material was completely consumed. The reaction mixture was concentrated in vacuo to give the crude product. The residue was purified by flash silica gel chromatography (ISCO®; 40 g SepaFlash® Silica Flash Column, Eluent of 15-20% Ethyl acetate/Petroleum ethergradient @ 45 mL/min). Compound 1-(10-bromodecyl)-2,3,4,5-tetramethoxy-6-methyl-benzene (150 mg, 347.70 umol, 44.25% yield) was obtained as a colorless oil.

MS (ESI): mass calcd. for C ₂₁ H ₃₅ BrO ₄ , 430.17; m/z found, 433.2 [M+3]+.

Step C. Formic acid, triphenyl (10-(2,3,4,5-tetramethoxy-6-methylphenyl)decyl) phosphonium salt

1-(10-bromodecyl)-2,3,4,5-tetramethoxy-6-methyl-benzene (130 mg, 301.34 umol, 1 eq) and PPh3 (344.51 mg, 1.31 mg) in toluene (1 mL) mmol, 4.36 eq) was heated at 130 °C under N ₂ for 18 h. LCMS showed that the starting material was completely consumed. The reaction mixture was concentrated in vacuo to give crude product. The residue was purified by prep-HPLC (FA condition).column: Xtimate C18 100*30mm*10um;mobile phase: [water(0.225%FA)-ACN];B%: 40%-70%,10min. The compound triphenyl-[10-(2,3,4,5-tetramethoxy-6-methyl-phenyl)decyl]phosphonium (64.7 mg, 103.30 umol, 34.28% yield, 98% purity) was obtained as a yellow oil. lost.

MS (ESI): mass calcd. for C ₃₉ H ₅₀ O ₄ P+, 613.34; m/z found, 613.6 [M+H]+.

1H NMR (400 MHz, DMSO-d6) δ ppm 1.17 - 1.37 (m, 12 H) 1.40 - 1.59 (m, 4 H) 2.04 - 2.11 (m, 3 H) 2.49 (br s, 2 H) 3.55 - 3.60 (m, 2 H) 3.65 - 3.68 (m, 3H) 3.69 - 3.72 (m, 3 H) 3.76 - 3.81 (m, 6 H) 7.72 - 7.84 (m, 12 H) 7.87 - 7.97 (m, 3 H) 8.21 - 8.43 (m, 1H)

1-7. Synthesis of formic acid, (10-(4,5-dimethoxy-2-methylphenyl)decyl)triphenylphosphonium salt

Step A. 10-Bromodetanoyl Chloride

To a mixture of 10-bromodecanic acid (500 mg, 1.99 mmol, 1 eq) in DCM (4 mL) was added SOCl ₂ (947.36 mg, 7.96 mmol, 577.66 uL, 4 eq). The reaction mixture was stirred at 25°C for 2 hours. TLC showed that the starting material was consumed completely. The reaction mixture was concentrated under vacuum. The crude product was not purified. Compound 10-bromodecanoyl chloride (500 mg, crude) was obtained as an orange oil.

Step B. 10-Bromo-1-(4,5-dimethoxy-2-methylphenyl)decan-1-one

AlCl ₃ to a solution of 1,2-dimethoxy-4-methyl-benzene (250 mg, 1.64 mmol, 1 eq) and 10-bromodecanoyl chloride (487.17 mg, 1.81 mmol, 1.1 eq) in DCE (10 mL). (197.13 mg, 1.48 mmol, 80.79 uL, 0.9 eq) was added. The mixture was stirred at 25 °C for 16 hours. LCMS showed that the starting material was consumed completely. The reaction mixture was quenched with H ₂ O (10 mL) and the mixture was filtered. The reaction filtrate was extracted with DCM (20 mL*3). The organic layer was separated, dried over Na ₂ SO ₄ , filtered and concentrated under reduced pressure to give a residue. The residue was purified by flash silica gel chromatography (ISCO®; 12 g SepaFlash® Silica Flash Column, Eluent of 0-20% Ethyl acetate/Petroleum ethergradient @ 80 mL/min). Compound 10-bromo-1-(4,5-dimethoxy-2-methyl-phenyl)decan-1-one (300 mg, 737.59 umol, 44.90% yield, 94.740% purity) was obtained as a white solid.

MS (ESI): mass calcd. for C ₁₉ H ₂₉ BrO ₃ , 384.13; m/z found, 387.0 [M+3]+.

1H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.32 - 1.38 (m, 2 H) 1.52 - 1.69 (m, 4 H) 1.78 (quin, J=7.13 Hz, 4 H) 2.28 (t, J=7.50 Hz) , 2 H) 2.42 (s, 3 H) 2.79 (t, J=7.38 Hz, 2 H) 3.33 (t, J=6.82 Hz, 2 H) 3.84 (d, J=4.25 Hz, 6 H) 6.59 - 6.70 (m, 1 H) 7.10 - 7.17 (m, 1 H)

Step C. 1-(10-Bromodecyl)-4,5-dimethoxy-2-methylbenzene

Et ₃ SiH (1.82 g, 15.65 mmol, 2.50 mL, 24.13 eq) was added. The mixture was stirred at 80 °C for 2 hours. LCMS showed that the starting material was completely consumed. The reaction mixture was concentrated under vacuum to give crude product. The residue was purified by flash silica gel chromatography (ISCO®; 20 g SepaFlash ® Silica Flash Column, Eluent of 0-15% Ethyl acetate/Petroleum ether gradient @ 80 mL/min). Compound 1-(10-bromodecyl)-4,5-dimethoxy-2-methyl-benzene (130 mg, 350.07 umol, 53.96% yield) was obtained as a colorless oil.

MS (ESI): mass calcd. for C ₁₉ H ₃₁ BrO ₂ , 370.15; m/z found, 371.2 [M+H]+.

1H NMR (400 MHz, CHLOROFORM-d) δ ppm 1.14 - 1.35 (m, 12 H) 1.41 (dt, J=15.10, 7.65 Hz, 2 H) 1.73 (quin, J=7.16 Hz, 2 H) 2.09 - 2.19 (m, 3 H) 2.36 - 2.43 (m, 2 H) 3.28 (t, J=6.82 Hz, 2 H) 3.72 (d, J=3.13 Hz, 6 H) 6.43 - 6.62 (m, 2 H)

Step D. Formic acid, (10-(4,5-dimethoxy-2-methylphenyl)decyl)triphenylphosphonium salt

PPh ₃ (353.16 mg, 1.35 mmol, 5 eq) was added. The mixture was stirred at 130 °C for 24 hours. LCMS showed that the starting material was consumed completely. The reaction mixture was concentrated under vacuum to give crude product. The residue was purified by prep-HPLC (FA condition:column: Phenomenex Luna C18 75*30mm*3um;mobile phase: [water(0.225%FA)-ACN];B%: 35%-70%,35min), The compound (40 mg, purity 93.747%) was obtained as a white solid, which was obtained by prep-HPLC (condition: column: Phenomenex Luna C18 75*30mm*3um; mobile phase: [water(0.225%FA)-ACN];B% : 35%-70%, 35min) was further separated. Compound 10-(4,5-dimethoxy-2-methyl-phenyl)decyl-triphenyl-phosphonium (17 mg, 27.41 umol, 10.18% yield, 96.703% purity, FA) was obtained as a colorless oil.

MS (ESI): mass calcd. for C ₃₇ H ₄₆ O ₂ P+, 553.32; m/z found, 553.5 [M+H]+.

1H NMR (400 MHz, DMSO-d6) δ ppm 1.16 - 1.31 (m, 10 H) 1.38 - 1.60 (m, 6 H) 2.16 (s, 3 H) 2.40 - 2.48 (m, 2 H) 3.56 - 3.60 ( m, 2 H) 3.69 (d, J=2.75 Hz, 6 H) 6.68 (s, 1 H) 6.72 (s, 1 H) 7.74 - 7.85 (m, 12 H) 7.87 - 7.94 (m, 3 H) 8.44 (s, 1H)

1-8. Synthesis of (10-(3-methyl-1,4-dioxo-1,4-dihydronaphthalen-2-yl)decyl)triphenylphosphonium

Preparation procedure for compound 2

Cpd.1 (10.0 g, 58.0 mmol, 1.00 eq) and Cpd.2B (12.9 g, 63.8 mmol, 1.10 eq) were added, AgNO ₃ (9.87 g, 58.0 mmol, 1.00 eq) was added to ACN (100 mL) and It was placed in a round bottom flask filled with H ₂ O (100 mL). To the mixture was added dropwise a solution of (NH) ₄ S ₂ O ₈ (15.9 g, 69.6 mmol, 15.1 mL, 1.20 eq) in H ₂ O (100 ml). The mixture was stirred for 4 hours at 75 °C in the dark. LCMS (ET36187-5-P1L, Cpd.2: RT = 1.431 min) showed partially consumed Cpd.1 and formed Cpd.2. The mixture was cooled to 20 °C. The mixture was extracted with EtOAc (100.0 mL x 3). The organic layer was washed with NaHCO3 (50.0 mL) and brine (50.0 mL). The organic layer was concentrated in vacuo. The residue was purified by column chromatography (SiO2, Petroleum ether/Ethyl acetate = 1/0 to 0/1). Cpd.2 (5.91 g, 17.9 mmol, 30.9% yield) was obtained as a white solid.

Preparation procedure for compound 3

To a 3 neck round bottom flask charged with DCM (200.0 mL) was added Cpd.2 (2.00 g, 6.09 mmol, 1.00 eq). CBr4 (2.42 g, 7.31 mmol, 1.20 eq) and PPh3 (1.92 g, 7.31 mmol, 1.20 eq) were added to the mixture. The reaction was stirred at 20 °C for 2 hours. TLC (Petroleum ether/Ethyl acetate = 15/1 Rf = 0.53) showed consumed Cpd.2 and formed Cpd.3. LCMS (ET36249-7-p1a, Cpd.3: RT = 1.678 min) showed that Cpd.3 was formed. Concentrated in vacuo. The residue was purified by column chromatography (SiO2, Petroleum ether/Ethyl acetate = 2/1 to 15/1). Cpd.3 (1.10 g, 2.81 mmol, 46.1% yield) was obtained as a yellow oil.

1H NMR: ET36249-7-P1a (400 MHz, CDCl3).

δ 8.07~8.09 (m, 2H), 7.68~7.70 (m, 2H), 3.41 (t, J = 4 Hz, 2H), 2.63 (t, J = 8 Hz, 2H), 2.20 (s, 3H), 1.48~1.87 (m, 2H), 1.30~1.46(m, 15H).

Preparation procedure of the target compound

Cpd.3 (0.20 g, 511 umol, 1.00 eq) was prepared by Tol. (1.40 mL) was added to a one-necked round bottom flask. PPh3 (160 mg, 613 umol, 1.20 eq) was added to the mixture. It was degassed 3 times with N2. Stirred at 120 °C for 16 hours. TLC (Dichloromethane/Methanol = 10/1, Rf = 0.50) showed that Cpd.3 was consumed and the reaction was complete. HPLC (ET36249-13-p1e) showed that the target was 91.2% pure. Concentrated in vacuo to Tol. (1.40 mL) was removed. The residue was taken up with MeOH (4.00 ml). The residue was purified by prep-TLC (Dichloromethane/Methanol = 10/1). The target (12.0 mg, 18.3 umol, 3.59% yield) was obtained as an orange gum.

1H NMR: ET36249-13-P1d (400 MHz, MeOD-d4).

δ 7.88~8.04 (m, 2H), 7.75~7.87 (m, 15H), 3.42~3.35 (m, 2H), 2.63 (t, J = 8 Hz, 2H), 2.16 (s, 3H), 1.28~1.69 (m, 16H).

1-9. Synthesis of triphenyl(10-phenyldecyl)phosphonium chloride

Preparation procedure for compound 2

Cpd.1 (3.00 g, 10.0 mmol, 1.00 eq) was added to THF (7.50 mL) at 0 °C. The mixture was degassed 3 times with N2. PhLi (1.80 M, 1.83 mL, 0.33 eq) was added dropwise to the solution at 0 °C. Stir at 0 °C for 1 hour, then warm to 15 °C. Stirred at 15 °C for 48 hours. TLC (Petroleum ether : Ethyl acetate= 1 : 0, Rf (Cpd.2) = 0.60) showed that Cpd.1 was consumed and the reaction was complete. Concentrated in vacuo. No further purification was performed. Cpd.2 (2.30 g, crude) was obtained as a colorless oil.

1H NMR: ET41362-1-P1C1 (400 MHz, CDCl3)

δ 7.27-7.26 (m, 2H), 7.19-7.17 (d, J = 8 Hz, 2H), 1.89-1.82 (m, 15H), 1.59-1.52 (m, 9H), 1.30 (s, 32H), 0.94 -0.90 (t, J = 8 Hz, 12H).

Procedure for the preparation of triphenyl(10-phenyldecyl)phosphonium chloride

Cpd.2 (0.70 M, 11.0 mL, 1.00 eq) was added in toluene (27.0 mL) at 15 °C. PPh3 (4.06 g, 15.4 mmol, 2.00 eq) was added to the solution. The mixture was degassed with N 3 times and heated to 100 °C. Stir at 100 °C for 12 hours. TLC (Dichloromethane : Methanol= 20 : 1, Rf (target 1) = 0.30) showed that Cpd.2 was consumed and the reaction was complete. Concentrated in vacuo. The crude product was purified by silica gel chromatography (Dichloromethane : Methanol = 100 : 0 to 20 : 1). Target 1 (27.0 mg, 54.8 umol, 7.09e-1% yield, 97.5% LCMS (ET41362-3-P1J1) purity) was obtained as a yellow oil.

1H NMR: ET41362-3-P1J1 (400 MHz, CDCl3)

δ 7.79–7.71 (d, J = 32 Hz, 15H), 7.28–7.16 (m, 6H), 3.95–3.74 (m, 4H), 2.59–2.55 (t, J = 8 Hz, 2H), 1.62–1.56 (m, 4H), 1.25–1.20 (d, J = 20 Hz, 10H).

1-10. Synthesis of (10-cyclohexyldecyl)triphenylphosphonium chloride

Preparation procedure for compound 3

In THF (3.00 mL) was added Cpd.1 (3.00 g, 10.0 mmol, 1.00 eq) at 0 °C. The mixture was degassed with N ₂ three times. CuLi ₂ C _l4 (0.10 M, 999 uL, 0.01 eq) was added to the solution. Cpd.1a (1.00 M, 12.0 mL, 1.20 eq) was added dropwise to the solution at 0 °C. Stirred at 0 °C for 1 hour and warmed to 15 °C. Stirred at 15 °C for 20 hours. TLC (Petroleum ether : Ethyl acetate= 1 : 0, Rf (Cpd.3) = 0.80) showed that Cpd.1 was consumed and the reaction was complete. Concentrated in vacuo. No further purification was performed. Cpd.3 (3.90 g, crude) was obtained as a colorless oil.

1H NMR: ET41362-2-P1C1 (400 MHz, CDCl3)

δ 3.43–3.40 (t, J = 8 Hz, 1H), 1.70–1.67 (m, 15H), 1.20–1.12 (m, 14H), 0.90–0.84 (m, 8H).

Procedure for the preparation of (10-cyclohexyldecyl)triphenylphosphonium chloride

Cpd.3 (0.70 M, 18.3 mL, 1.00 eq) was added in toluene (16.0 mL) at 15 °C. PPh ₃ (6.74 g, 25.7 mmol, 2.00 eq) was added to the solution. The mixture was degassed 3 times with N ₂ and heated to 100°C. Stir at 100 °C for 12 hours. TLC (Dichloromethane : Methanol= 20 : 1, Rf (target 2) = 0.20) showed that Cpd.3 was consumed and the reaction was complete. Concentrated in vacuo. The crude product was purified by silica gel chromatography (Dichloromethane : Methanol = 100 : 0 to 20 : 1). Target 2 (0.02 g, 40.4 umol, 3.14e-1% yield, 98.1% LCMS (ET41362-4-P1J1) purity) was obtained as a yellow oil.

1H NMR: ET41362-4-P1C1 (400 MHz, CDCl3)

δ 7.89–7.70 (m, 15H), 3.83 (s, 2H), 2.60 (s, 2H), 1.68–1.62 (m, 9H), 1.18 (s, 18H), 0.87–0.79 (m, 2H).

1-11. Preparation of (10-(3,4-dimethylphenyl)decyl)triphenyl phosphonium bromide.

Step A. 10-Bromodecanoyl Chloride

To a solution of 10-bromodecanic acid (30.0 g, 119 mmol, 1.00 eq) in DCM (210 mL) was added SOCl ₂ (56.8 g, 478 mmol, 34.7 mL, 4.00 eq). The mixture was stirred at 25 °C for 1 hour. TLC1 (Petroleum ether : ethyl acetate = 5 : 1, Rf (start material) = 0.30, Rf (product ) = 0.52) showed complete consumption of the starting material. The mixture was concentrated in vacuo. 10-bromodecanoyl chloride (30.0 g, crude) was obtained as a yellow oil.

Step B. 10-Bromo-1-(3,4-dimethylphenyl)decan-1-one

AlCl ₃ (5.65 g, 5.65 g, 42.4 mmol, 2.32 mL, 0.90 eq) was added. The mixture was stirred at 25 °C for 16 hours. TLC (Petroleum ether : Ethyl acetate = 5 : 1, starting material Rf = 0.70, Rf (product) = 0.88) showed complete consumption of the starting material. The mixture was poured into ice water (50.0 mL) and extracted with DCM (50.0 mL). The organic phase was separated and concentrated in vacuo. The residue was purified by column chromatography (SiO2, Petroleum ether : Ethyl acetate = 1 : 0 to 1 : 1). 10-Bromo-1-(3,4-dimethylphenyl)decan-1-one (6.00 g, 13.4 mmol, 28.3% yield, 75.5% purity) was analyzed by HNMR (ET47086-1-P1A2) and LCMS (ET47086-1 -P1A1, T = 0.996, M + 1 = 339.2) as a yellow solid.

1H NMR (400 MHz, CHLOROFORM-d) δ = 7.74 (s, 1H) 7.70 (dd, J = 7.60, 1.64 Hz, 1H) 7.21 (d, J = 7.60 Hz, 1H) 3.41 (t, J = 6.80 Hz , 2H) 2.93 (t, J = 7.60 Hz, 2H) 2.32 (s, 6H) 1.86 (quin, J = 7.20 Hz, 2H) 1.73 (quin, J = 7.20 Hz, 2H) 1.26 - 1.49 (m, 10H)

Step C. 4-(10-Bromodecyl)-1,2-dimethyl-benzene

To a solution of 10-bromo-1-(3,4-dimethylphenyl)decan-1-one (1.00 g, 2.95 mmol, 1.00 eq) in TFA (7.00 mL) Et ₃ SiH (8.57 g, 73.7 mmol, 11.8 mL, 25.0 eq) was added. The mixture was stirred at 80 °C for 2 h. TLC (Petroleum ether : ethyl acetate = 5 : 1, Rf (start material) = 0.7, Rf (product) = 0.6) showed complete consumption of the starting material. The mixture was poured into water (10.0 mL) then extracted with EtOAc (5.00 mL x 3) and the combined organic layers were washed with brine (10.0 mL). The residue was purified by column chromatography (SiO2, Petroleum ether : Ethyl acetate = 1 : 1 to 0 : 1). 4-(10-Bromodecyl)-1,2-dimethyl-benzene (0.20 g, 615 umol, 20.8% yield) was obtained as a colorless oil.

Step D: bromide 10- (3, 4-dimethylphenyl) decyl triphenyl phosphonium salt

Tol. (7.00 mL) was added PPh ₃ (806 mg, 3.07 mmol, 5.00 eq) . The mixture was stirred at 130 °C for 18 hours. TLC1 (Dichloromethane : Methanol = 5 : 1, Rf (product) = 0.4) and TLC2 (Petroleum ether : Ethyl acetate = 5 : 1, Rf (start material) = 0.8) showed complete consumption of starting materials. The mixture was concentrated in vacuo. The residue was triturated with PE : MTBE = 2 : 1 (1 mL) at 25 °C for 30 min. Bromide 10- (3, 4-dimethylphenyl) decyl triphenyl phosphonium salt (0.05 g, 84.2 umol, 13.6% yield, 98.9% purity, Br-) was obtained as a white solid, which was determined by HNMR (ET46959-5-P1B1) , confirmed by LCMS (ET46959-5-P1B1, T = 2.958, M+ = 507.2, HPLC (ET46959-5-P1A3, T = 4.121, Purity = 98.9%).

1 H NMR (400 MHz, DMSO-d6)

δ = 7.93 - 7.86 (m, 3H), 7.84 - 7.73 (m, 12H), 7.00 (d, J = 7.60 Hz, 1H), 6.92 (s, 1H), 6.86 (br d, J = 7.60 Hz, 1H) ), 3.56 (br t, J = 14.4 Hz, 2H), 2.48 - 2.43 (m, 2H), 2.16 (d, J = 5.20 Hz, 6H), 1.57 - 1.38 (m, 6H), 1.20 (br d, J = 15.2 Hz, 10H)

1-12. Preparation of 10-(2,5-dimethoxy-3,4-dimethyl-phenyl)decyl-triphenyl-phosphonium

Step A. 10-Bromo-1-(2,5-dimethoxy-3,4-dimethyl-phenyl) decan-1-one

To a solution of 2,3-dimethylbenzene-1,4-diol (11.0 g, 79.6 mmol, 1.00 eq) in EtOH (70.0 mL) was added KOH (11.2 g, 199 mmol, 2.50 eq) and dimethyl sulfate (25.1 g, 199 mmol, 18.9 mL, 2.50 eq) was added. The mixture was stirred at 0 °C for 3.5 hours. TLC1 (Petrileum ether : ethyl acetate = 5 : 1, Rf (start material) = 0.10, Rf (product ) = 0.70) showed complete consumption of the starting material. The mixture was poured into 3M HCl (100 mL), then extracted with PE (50.0 mL x 3), and the combined organic layers were washed with 1M HCl (50.0 ml), water (50.0 mL), brine (50.0 mL). The residue was purified by column chromatography (SiO2, Petroleum ether : Ethyl acetate = 1 : 0 to 0 : 1). 10-Bromo-1-(2,5-dimethoxy-3,4-dimethyl-phenyl)decan-1-one (6.00 g, 36.1 mmol, 45.3% yield) was obtained as a brown solid and was obtained by LCMS (ET46959- 3-P1A1, T = 0.795, M + H = 167.3) and HNMR (ET46959-3-P1A1).

1H NMR (ET46959-3-P1A1, 400 MHz, DMSO-d6)

δ = 6.71 (s, 2H), 3.70 (s, 6H), 2.06 (s, 6H)

Step B. 10-Bromo-1-(2,5-dimethoxy-3,4-dimethyl-phenyl)decan-1-one

1,4-dimethoxy-2,3-dimethylbenzene (1.00 g, 6.02 mmol, 1.14 mL, 1.00 eq) and 10-bromodecanoyl chloride (1.78 g, 6.62 mmol, 1.10 eq) in DCE (7.00 mL) To the solution was added AlCl ₃ (722 mg, 5.41 mmol, 296 uL, 0.90 eq). The mixture was stirred at 25 °C for 2 hours. TLC (Petroleum ether : ethyl acetate = 5 : 1, Rf (start material) = 0.83, Rf (product) = 0.72) showed complete consumption of the starting material. The reaction mixture was quenched with H ₂ O (10.0 mL) and the mixture was filtered. The reaction filtrate was extracted with DCM (20.0 mL x 3). The organic phase was separated, dried over Na ₂ SO ₄ , filtered and concentrated under reduced pressure to give a residue. The residue was purified by column chromatography (SiO2, Petroleum ether : Ethyl acetate = 1 : 0 to 0 : 1). 10-Bromo-1-(2,5-dimethoxy-3,4-dimethyl-phenyl)decan-1-one (500 mg, 1.25 mmol, 20.8% yield) was obtained as a yellow solid, which was obtained by HNMR (ET46959 -6-P1A1), and confirmed by LCMS (ET46985-6-P1A2, T = 1.011, M + H = 399.3).

1H NMR: (ET46959-6-P1A1, 400 MHz, DMSO-d6)

δ = 6.89 (s, 1H), 3.76 (s, 3H), 3.59 (s, 3H), 2.93 (t, J = 7.20 Hz, 2H), 2.16 (s, 3H), 2.11 (s, 3H), 1.39 - 1.24 (m, 16H)

Step C. 1-(10-Bromodecyl)-2,5-dimethoxy-3,4-dimethyl-benzene

Et ₃ SiH to a solution of 10-bromo-1-(2,5-dimethoxy-3,4-dimethyl-phenyl)decan-1-one (500 mg, 1.25 mmol, 1.00 eq) in TFA (5.00 mL). (3.64 g, 31.3 mmol, 5.00 mL, 25.0 eq) was added. The mixture was stirred at 80 °C for 2 hours. TLC (Petroleum ether : ethyl acetate = 5 : 1, Rf (start material) = 0.7, Rf (product) = 0.6) showed complete consumption of the starting material. The mixture was poured into water (10.0 mL), then extracted with EtOAc (5.00 mL x 3) and the combined organic layers were washed with brine (10.0 mL). The residue was purified by column chromatography (SiO2, Petroleum ether : Ethyl acetate = 1 : 0 to 0 : 1). 1-(10-bromodecyl)-2,5-dimethoxy-3,4-dimethyl-benzene (0.20 g, 519 umol, 41.4% yield) was obtained as a pale yellow oil, which was determined by HNMR (ET46959-8- P1A), and LCMS (ET46959-8-P1A, T = 1.055. M + H = 385.3).

1H NMR (ET46959-8-P1A, 400 MHz, DMSO-d6)

δ = 6.63 - 6.56 (m, 1H), 3.70 (s, 3H), 3.54 (s, 3H), 2.10 (s, 3H), 2.02 (s, 3H), 1.77 (q, J = 7.20 Hz, 2H) , 1.59 - 1.48 (m, 2H), 1.41 - 1.21 (m, 14H))

Step D. Formic acid, 10-(2,5-dimethoxy-3,4-dimethyl-phenyl)decyl-triphenyl-phosphonium salt

Tol. (3.50 mL) of PPh ₃ (272 mg, 1.04 mmol , 2.00 eq) was added. The mixture was stirred at 130 °C for 18 hours. TLC1 (Dichloromethane : Methanol = 5 : 1, Rf (product) = 0.4) and TLC2 (Petroleum ether : Ethyl acetate = 5 : 1, Rf (start material) = 0.8) showed complete consumption of starting materials. The mixture was concentrated in vacuo. The residue was purified by prep-HPLC (column: Phenomene x Luna C18 75 x 30mm x 3um; mobile phase: [water (FA) - ACN]; B%: 40% - 75%, 8min). Formic acid, 10- (2, 5-dimethoxy-3, 4-dimethyl-phenyl) decyl-triphenyl-phosphonium salt (30.0 mg, 52.5 umol, 10.1% yield, 99.3% purity) was obtained as a yellow gum; This was confirmed by HNMR (ET46959-9-P1A), LCMS (ET46959-9-P1A, T = 2.938. M+ = 567.2), and HPLC (ET46959-9-P1B, T = 4.052, Purity = 99.3%).

1H NMR (ET46959-9-P1A, 400 MHz, DMSO-d6)

δ = 8.52 (s, 1H), 7.92 - 7.86 (m, 3H), 7.83 - 7.74 (m, 12H), 6.61 - 6.56 (m, 1H), 3.70 (s, 3H), 3.54 (s, 5H), 2.10 (s, 3H), 2.02 (s, 3H), 1.56 - 1.38 (m, 7H), 1.30 - 1.15 (m, 11H)

1-13. Preparation of (10- (3, 4-dimethoxyphenyl) decyl) triphenylphosphonium bromide

Step 1: 10-Bromo-1-(3,4-dimethoxyphenyl)decan-1-one

To a solution of 1,2-dimethoxybenzene (5.00 g, 36.2 mmol, 4.63 mL, 1.00 eq) and 10-bromodecanoyl chloride (10.7 g, 39.8 mmol, 1.10 eq) in DCE (35.0 mL) was added AlCl ₃ ( 4.34 g, 32.6 mmol, 1.78 mL, 0.90 eq) was added. The mixture was stirred at 25° C. for 16 hours. The mixture was poured into ice water (50.0 mL) and extracted with DCM (50.0 mL). The organic phase was separated and concentrated in vacuo. The residue was purified by column chromatography (SiO2, Petroleum ether : Ethyl acetate = 1 : 0 to 1 : 1). 10-Bromo-1-(3,4-dimethoxyphenyl) decan-1-one (3.00 g, 7.92 mmol, 21.8% yield, 98.0% purity) was obtained as a white solid.

MS (ESI): mass calcd. for C18H27BrO3, 370.11; m/z found, 371.2 [M+H]+.

1H NMR (400 MHz, CDCl3-d)

δ = 7.59 (dd, J = 8.32, 1.96 Hz, 1H), 7.54 (d, J = 1.96 Hz, 1H), 6.89 (d, J = 8.44 Hz, 1H), 3.95 (d, J = 3.32 Hz, 6H) ), 3.41 (t, J = 6.84 Hz, 2H), 2.92 (t, J = 7.40 Hz, 2H), 1.85 (quin, J = 7.16 Hz, 2H), 1.66 - 1.78 (m, 2H), 1.27 - 1.50 (m, 10H)

Step 2: 4-(10-bromodecyl)-1,2-dimethoxybenzene

To a solution of 10-bromo-1- (3, 4-dimethoxyphenyl) decan-1-one (2.00 g, 5.39 mmol, 1.00 eq) in TFA (15.0 mL) was added Et ₃ SiH (15.7 g, 134 mmol, 21.5 mL, 25.0 eq) was added. The mixture was stirred at 80 °C for 1 hour. The mixture was concentrated in vacuo. The residue was purified by column chromatography (SiO2, Petroleum ether : Ethyl acetate = 1 : 0 to 10 : 1). 4-(10-Bromodecyl)-1,2-dimethoxy-benzene (1.00 g, 2.79 mmol, 51.7% yield, 99.5% purity) was obtained as a pale yellow oil.

MS (ESI): mass calcd. for C18H29BrO2, 356.14; m/z found, 357.2 [M+H]+.

Step 3: (10-(3,4-dimethoxyphenyl)decyl)triphenylphosphonium bromide

Tol. (1.40 mL) of 4-(10-bromodecyl)-1,2-dimethoxy-benzene (200 mg, 559 umol, 1.00 eq) was added PPh ₃ (161 mg, 615 umol, 1.10 eq) It became. The mixture was stirred at 130 °C for 12 hours. The mixture was concentrated in vacuo. The crude product was triturated with MeCN (2.00 mL) at 20 °C for 1 hour and then purified in neutral condition. 10-(3,4-dimethoxyphenyl)decyl-triphenyl-phosphonium (50.0 mg, 92.3 umol, 16.4% yield, 99.6% purity) was obtained as a yellow gum.

MS (ESI): mass calcd. for C36H44BrO2P, 618.23; m/z found, 539.2 [M]+.

1 H NMR (400 MHz, DMSO-d6)

δ = 7.85 - 7.95 (m, 3H), 7.73 - 7.84 (m, 12H), 6.82 (d, J = 8.16 Hz, 1H), 6.75 (d, J = 1.84 Hz, 1H), 6.66 (dd, J = 8.08, 1.83 Hz, 1H), 3.70 (d, J = 7.96 Hz, 6H), 3.48 - 3.63 (m, 2H), 2.47 (br s, 2H), 2.07 (s, 1H), 1.37 - 1.59 (m, 6H), 1.10 - 1.32 (m, 10H)

1-14. Mito-CP (2,2,5,5-tetramethyl-3-(((10-(triphenylphosphonio)decyl)oxy)carbonyl)pyrrolidin-1-olate) and Mito-CP ^S Preparation of (2,2,5,5-tetramethyl-3-((2-(triphenylphosphonio)ethoxy)carbonyl)pyrrolidin-1-olate).

(2-hydroxyethyl)triphenylphosphonium (6a).

A mixture of 2-bromoethanol (1.5 mmol), PPh ₃ (1.0 mmol), and acetonitrile (20 mL) was refluxed for 24 hours. After cooling to room temperature, the solvent was removed via rotary evaporation. The residual light-yellow oil was washed twice with ether to give the phosphonium salt 6a. Yield: 77%; 1H NMR (400 MHz, CDCl3) δ 7.95 - 7.56 (m, 15H), 5.21 (s, 1H), 4.14 (d, J = 16.6 Hz, 2H), 3.83 (s, 2H).

(10-hydroxydecyl)triphenylphosphonium (6b).

The synthetic procedure of 6a was applied with 10-bromodecanol (1.5 mmol) to give the product as a brown oil. Yield: 85%; 1H NMR (400 MHz, CDCl3) δ 7.92 - 7.83 (m, 6H), 7.79 (dd, J = 7.7, 5.8 Hz, 3H), 7.70 (m, 6H), 3.91 - 3.81 (m, 2H), 3.63 ( t, J = 6.6 Hz, 2H), 1.69 - 1.50 (m, 12H), 1.31 (m, 4H).

2,2,5,5-Tetramethyl-3-((2-(triphenylphosphonio)ethoxy)carbonyl)pyrrolidin-1-olate (Mito-CPs; 8).

To a solution of 3-carboxy-2,2,5,5-tetramethylpyrrolidin-1-olate (7, 1.0 mmol) in benzene was added pyridine (1.0 mmol). The flask was continued to cool on an ice bath and thionyl chloride (2.0 mmol) was added dropwise over 1 hour. The solvent was removed via vacuum evaporation. The resulting residue and (2-hydroxyethyl)triphenylphosphonium (6a, 1.0 mmol) were dissolved in dichloromethane (10 mL). To this solution, pyridine (1.0 mmol) was added dropwise under an ice bath and stirred at room temperature for 6 hours. The reaction mixture was quenched with saturated aqueous NaHCO ₃ solution and extracted with ethyl acetate (60 ml x 3). The combined organic layers were washed with brine, dried over Na ₂ SO ₄ , filtered and evaporated in vacuo. The crude product was purified by MPLC (MeOH 5% in DCM) to give the compound (21%); HRMS (ESI, m/z) calculated for C ₂₉ H ₃₄ NO ₃ P [M]+ 475.2276, found 475.2260.

2,2,5,5-Tetramethyl-3-(((10-(triphenylphosphonio)decyl)oxy)carbonyl)pyrrolidine-1-olate (Mito-CP; 9).

The synthesis procedure of 8 was applied with (10-hydroxydecyl)triphenylphosphonium (6b, 1.0 mmol) to give 9 as a brown oil. Yield: 24%; HRMS (ESI, m/z) calculated for C ₃₇ H ₅₀ NO ₃ P [M]+ 587.3528, found 587.3524.

1-15. MitoQ ^S :((4,5-dimethoxy-2-methyl-3,6-dioxocyclohexa-1,4-dien-1-yl)methyl)triphenylphosphonium bromide preparation.

1-(Bromomethyl)-2,3,4,5-tetramethoxy-6-methylbenzene (2).

1,2,3,4-tetramethoxy-5-methylbenzene (2.721 g, 12.7 mmol) and paraformaldehyde (0.763 g, 25.4 mmol) were dissolved in 47% HBr (10 mL). The mixture was then stirred at 40° C. for 2 h and left at room temperature. After the reaction was complete, the product was extracted with hexanes (40 mL) and the combined organic layers were dried over sodium sulfate. The remaining solvent was removed in vacuo to give a light yellow oil 2 (yield: 88%); 1H NMR (400 MHz, CDCl3) δ 4.61 (s, 2H), 3.95 (s, 3H), 3.93 (s, 3H), 3.89 (s, 3H), 3.79 (s, 3H), 2.27 (s, 3H) ; 13C NMR (100 MHz, CDCl3) δ 148.5, 148.0, 147.8, 144.7, 126.6, 124.9, 61.3, 61.1, 61.1, 60.8, 26.6, 11.1.

2-(Bromomethyl)-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione (3).

Intermediate 2 (211 mg, 0.687 mmol) was dissolved in THF (10 mL). Ammonium cerium(IV) nitrate (1.5 g, 2.74 mmol) in water (10 mL) was then added to the reaction mixture. The mixture was stirred at room temperature for 2 hours. After completion of the reaction, the resulting mixture was extracted with DCM (20 mL) and the extract was washed with brine until neutral. The combined organic layers were then dried over sodium sulfate and the remaining solvent removed in vacuo. The residue was purified by silica gel chromatography to give a yellow oil 3 (yield: 45%); 1H NMR (400 MHz, CDCl3) δ 5.39 (s, 2H), 4.03 (s, 3H), 4.02 (s, 3H), 2.17 (s, 3H); 13C NMR (100 MHz, CDCl3) δ 183.9, 181.6, 145.0, 144.5, 141.7, 137.6, 61.4, 61.3, 21.5, 12.0; MS (ESI, m/z) calculated for C ₁₀ H ₁₂ BrO ₄ [M+H]+ 274.99, found 275.00.

((4,5-dimethoxy-2-methyl-3,6-dioxocyclohexa-1,4-dien-1-yl)methyl)triphenylphosphonium (MitoQs; 4).

3 (22.31 mg, 0.0726 mmol) and triphenylphosphine (57.1 mg, 0.218 mmol) were dissolved in ACN (10 mL) and the reaction mixture was refluxed overnight. After allowing to room temperature, the remaining solvent was removed in vacuo. The residue was purified by silica gel chromatography to give a white solid 4 (yield: 18%); 1H NMR (400 MHz, CD3OD) δ 7.88-7.81 (m, 3H), 7.70-7.64 (m, 6H), 7.62-7.55 (m, 6H), 3.80 (s, 3H), 3.52 (s, 3H), 3.34 (s, 2H), 1.81 (d, J = 1.5 Hz, 3H); 13C NMR (101 MHz, MeOD) δ 182.74 (d, J = 3.3 Hz), 182.65 (d, J = 2.3 Hz), 145.80, 145.70, 145.21, 143.96, 135.27 (d, J = 3.0 Hz), 134.30 (d , J = 10.1 Hz), 130.37 (d, J = 12.8 Hz), 118.14 (d, J = 85.9 Hz), 61.31, 61.26, 24.81 (d, J = 49.8 Hz), 14.68 (d, J = 2.7 Hz) ; HRMS (ESI, m/z) calculated for C ₂₈ H ₂₆ O ₄ P [M]+ 457.1563, found 457.1566.

1-16. Prepare Mito-VitE:2-(6-hydroxy-2,5,7,8-tetramethylchroman-2-yl)ethyl)triphenylphosphonium.

4-(tetrahydro-2H-pyran-2-yl)butan-2-one (11).

To a solution of 4-hydroxybutan-2-one (11.35 mmol) and 3,4-dihydro-2H-pyran (15.89 mmol) in CH ₂ Cl ₂ (15 ml), pyridinium p-toluenesulfonate (1.135 mmol.0.1 eq) was added and stirred at room temperature for 4 hours. The reaction mixture was then concentrated in vacuo and the residue was dissolved in Et2O (50 mL). It was washed with saturated aqueous NaCl (40 mL) and H ₂ O (10 mL), dried over anhydrous MgSO ₄ , filtered and concentrated in vacuo to give compound 11. Yield: 64%; 1H NMR (400 MHz, CDCl3) δ 4.60 (t, J = 3.4 Hz, 1H), 4.00 (dt, J = 10.2, 6.2 Hz, 1H), 3.89 - 3.80 (m, 1H), 3.69 (dt, J = 10.1, 6.3 Hz, 1H), 3.55 - 3.45 (m, 1H), 2.71 (t, J = 6.2 Hz, 2H), 2.20 (s, 3H), 1.83 - 1.64 (m, 3H), 1.60 - 1.54 (m , 3H).

3-methyl-5-((tetrahydro-2H-pyran-2-yl)oxy)pent-1-en-3-ol (12).

Vinylmagnesium bromide (13.35 mmol) was added to a solution of 11 (5.81 mmol) in THF (25 ml) at -78 °C. It was stirred for 2 hours and then warmed to room temperature over 30 minutes. Saturated aqueous NH ₄ Cl (50 mL) was added dropwise and it was extracted with Et ₂ O. The combined organic phases were washed with saturated aqueous NaCl, dried over anhydrous MgSO ₄ , filtered and concentrated to give a pale yellow oil. The crude product was purified by MPLC. Yield: 93%; 1H NMR (400 MHz, CDCl3) δ 5.90 (ddd, J = 16.7, 10.7, 5.8 Hz, 1H), 5.38 - 5.27 (m, 2H), 5.10 (ddd, J = 10.3, 8.7, 1.4 Hz, 1H), 4.67 - 4.53 (m, 1H), 3.97 (dt, J = 9.7, 4.2 Hz, 1H), 3.83 (td, J = 11.5, 5.6 Hz, 1H), 3.59 - 3.45 (m, 3H), 1.97 (m, 1H), 1.80 - 1.72 (m, 2H), 1.59 - 1.49 (m, 3H), 1.29 (t, J = 5.0 Hz, 3H).

2-(2-hydroxyethyl)-2,5,7,8-tetramethylchroman-6-ol (13).

A solution of 12 (5.39 mmol) and freshly prepared 2,3,5-trimethylbenzene-1,4-diol (4.49 mmol) in formic acid (10 ml) was refluxed under a nitrogen atmosphere for 3.5 hours. The reaction mixture was poured onto crushed ice and the organics were extracted with Et ₂ O. The combined organic phases were washed with H ₂ O, dried over anhydrous MgSO ₄ and concentrated in vacuo. The brown oily residue was mixed with MeOH and conc. Dissolved in HCl and refluxed under argon for 30 minutes. After removing the solvent in vacuo, the residue was dissolved in Et ₂ O. Washed again with H ₂ O, saturated aqueous NaHCO ₃ , H ₂ O under argon, dried over anhydrous MgSO ₄ , filtered and concentrated to give a brown oil. The crude product was purified by MPLC to give the compound. Yield: 35%; 1H NMR (400 MHz, CDCl3) δ 5.30 (s, 1H), 4.23 (s, 1H), 3.97 - 3.86 (m, 2H), 2.66 (dd, J = 9.8, 6.2 Hz, 2H), 2.17 (s, 3H), 2.12 (s, 3H), 2.09 (s, 3H), 1.97 (m, 2H), 1.93 - 1.82 (m, 2H), 1.29 (s, 3H).

2,5,7,8-tetramethyl-2-(2-((methylsulfonyl)oxy)ethyl)chroman-6-yl methanesulfonate (14).

To a solution of 13 (0.479 mmol) and triethylamine (2.88 mmol) in CH ₂ Cl ₂ (2 ml) was added methanesulfonyl chloride (1.055 mmol) and stirred at room temperature for 1 hour. The reaction mixture was diluted with CH ₂ Cl ₂ (20 mL). It was washed with H ₂ O, dried over anhydrous MgSO ₄ , filtered and concentrated in vacuo to give a white solid. It was recrystallized from EtOH to give the product. Yield: 43%; 1H NMR (400 MHz, CDCl3) δ 4.56 - 4.45 (m, 2H), 4.41 (dd, J = 14.8, 8.7 Hz, 2H), 3.25 (s, 3H), 3.00 (s, 3H), 2.63 (t, J = 7.0 Hz, 2H), 2.23 (d, J = 13.2 Hz, 6H), 2.09 (s, 3H), 1.86 (t, J = 6.8 Hz, 2H), 1.31 (s, 3H).

Triphenyl(2-(2,5,7,8-tetramethyl-6-((methylsulfonyl)oxy)chroman-2-yl)ethyl)phosphonium (15).

A mixture of 14 (0.256 mmol), sodium iodide (192 mg, 1.279 mmol), and triphenylphosphine (1.279 mmol) was flushed with argon in a Kimax tube, then sealed and reacted as a melt at 90 °C for 48 h. Stir. The crude product was dissolved in CH ₂ Cl ₂ and precipitated from petroleum ether three times. The product was dissolved in methanol and passed through an anion exchange column loaded with -Oms. Residual solvent was removed in vacuo to give pure product 15. Yield: 39%; 1H NMR (400 MHz, CDCl3) δ 7.87 - 7.72 (m, 9H), 7.66 (td, J = 7.9, 3.4 Hz, 6H), 4.12 (m, 2H), 3.28 (s, 3H), 2.61 - 2.49 ( m, 2H), 2.25 (s, 3H), 2.15 (s, 3H), 2.05 (s, 2H), 2.03 (s, 3H), 2.00 (d, J = 6.5 Hz, 2H), 1.49 (s, 3H) ).

(2-(6-hydroxy-2,5,7,8-tetramethylchroman-2-yl)ethyl)triphenylphosphonium (Mito-VitE; 16).

Lithium diisopropylamide (0.119 mmol, 1 M solution in THF) was added to a solution of 15 (0.100 mmol) in THF (2 ml) at 0 °C. After 30 min the solution was warmed to room temperature and then aqueous saturated NH ₄ OMs (10 mL) was added. The aqueous layer was extracted three times with CH ₂ Cl ₂ and the combined organic phases were dried over anhydrous MgSO ₄ , filtered and concentrated in vacuo. The residue was purified by MPLC to give product 16. Yield: 9%; 1H NMR (400 MHz, CDCl3) δ 7.85 - 7.73 (m, 4H), 7.71 - 7.58 (m, 11H), 3.77 - 3.64 (m, 2H), 3.41 - 3.28 (m, 2H), 2.17 (s, 3H) ), 2.07 (d, J = 11.7 Hz, 6H), 1.94 (m, 4H), 1.34 (d, J = 7.6 Hz, 3H); HRMS (ESI, m/z) calculated for C ₃₃ H ₃₆ O ₂ P [M]+ 495.2447, found 495.2445.

1-17. Mito-VitE ^L : Preparation of (10-(6-hydroxy-2,5,7,8-tetramethylchroman-2-yl)decyl)triphenylphosphonium iodide

11-((tetrahydro-2H-pyran-2-yl)oxy)undecylic acid (18).

of 11-hydroxyundecylic acid (17, 11.35 mmol), 3,4-dihydro-2H-pyran (15.89 mmol), and pyridinium p-toluenesulfonate (1.135 mmol) in CH ₂ Cl ₂ (15 ml). The solution was stirred at room temperature for 4 hours. The reaction mixture was concentrated in vacuo and the residue was dissolved in Et2O (50 mL). It was washed with saturated aqueous NaCl (40 mL) and H ₂ O (10 mL), dried over anhydrous MgSO ₄ , filtered, and concentrated in vacuo to give 18 . Yield: 46%; 1H NMR (400 MHz, CDCl3) δ 4.63 - 4.48 (m, 1H), 3.88 (ddd, J = 11.1, 7.6, 3.3 Hz, 1H), 3.75 - 3.64 (m, 1H), 3.55 - 3.48 (m, 1H) ), 3.38 (dt, J = 9.6, 6.7 Hz, 1H), 2.34 (t, J = 7.5 Hz, 2H), 1.91 - 1.44 (m, 14H), 1.32 (d, J = 24.9 Hz, 8H).

N-methoxy-N-methyl-11-((tetrahydro-2H-pyran-2-yl)oxy)undecanamide (19). To a solution of 18 (5.23 mmol) in CH ₂ Cl ₂ (10 mL), N,O-dimethylhydroxylamine hydrochloride (5.75 mmol) and N-methylmorpholine (5.75 mmol) were added successively at -15 °C. added. After 10 min, N-(3-dimethylaminopropyl)-N'-decylcarbodiimide hydrochloride (1.62 g, 5.75 mmol) was added in portions over 15 min and stirred at -15 °C for 3 h. The reaction was quenched by addition of 1M HCl (5 mL) and the organics were extracted with CH ₂ Cl ₂ (3 x 50 mL). The organic extracts were combined and washed with saturated NaHCO ₃ solution (50 mL), dried over MgSO ₄ , filtered and evaporated to give the desired amide 19 (yield: 80%), which was used in the next step without further purification. 1H NMR (400 MHz, CDCl3) δ 4.60 (dd, J = 20.5, 17.9 Hz, 1H), 3.87 (ddd, J = 11.1, 7.4, 3.5 Hz, 1H), 3.79 - 3.65 (m, 4H), 3.50 ( dt, J = 5.1, 4.5 Hz, 1H), 3.38 (dt, J = 9.6, 6.7 Hz, 1H), 3.18 (s, 3H), 2.41 (t, J = 7.6 Hz, 2H), 1.90 - 1.78 (m , 1H), 1.76 - 1.47 (m, 14H), 1.29 (s, 8H).

12-((tetrahydro-2H-pyran-2-yl)oxy)dodecan-2-one (20).

To a solution of 19 (4.18 mmol) in Et ₂ O (13 mL) was added MeMgI (10.45 mmol) at 0 °C. After stirring at the same temperature for 3 h, saturated aqueous NH ₄ Cl solution (30 mL) was added to the reaction mixture. The organic layer was separated and the aqueous layer was extracted with tBuOMe (3 Х 30 mL). The combined organic layers were dried over Na ₂ SO ₄ and concentrated under reduced pressure. The residue was purified by flash chromatography to give compound 20. Yield: 80%; 1H NMR (400 MHz, CDCl3) δ 4.61 - 4.54 (m, 1H), 3.87 (ddd, J = 11.1, 7.4, 3.4 Hz, 1H), 3.77 - 3.66 (m, 1H), 3.55 - 3.46 (m, 1H) ), 3.38 (dt, J = 9.6, 6.7 Hz, 1H), 2.41 (t, J = 7.5 Hz, 2H), 2.13 (s, 3H), 1.90 - 1.76 (m, 1H), 1.72 (ddd, J = 11.9, 6.0, 3.2 Hz, 1H), 1.63 - 1.47 (m, 14H), 1.37 - 1.20 (m, 6H).

3-methyl-13-((tetrahydro-2H-pyran-2-yl)oxy)tridec-1-en-3-ol (21).

Vinylmagnesium bromide (8.3 mmol, 2.5 eq) was added to a solution of 20 (3.32 mmol) in THF (15 ml) at -78 °C. The solution was stirred for 2 hours and then allowed to warm to room temperature over 30 minutes. Saturated aqueous NH ₄ Cl (50 mL) was added dropwise and the organics were extracted with Et ₂ O. The combined organic layers were washed with saturated aqueous NaCl, dried over anhydrous MgSO ₄ , filtered, concentrated and purified by MPLC to give compound 21. Yield: 93%; 1H NMR (400 MHz, CDCl3) δ 5.91 (dd, J = 17.4, 10.8 Hz, 1H), 5.20 (dd, J = 17.4, 1.2 Hz, 1H), 5.04 (dd, J = 10.8, 1.2 Hz, 1H) , 4.64 - 4.51 (m, 2H), 3.87 (ddd, J = 11.0, 7.4, 3.3 Hz, 2H), 3.77 - 3.65 (m, 2H), 3.56 - 3.46 (m, 2H), 3.38 (dt, J = 9.5, 6.7 Hz, 2H), 1.90 - 1.77 (m, 2H), 1.71 (tt, J = 16.3, 7.0 Hz, 2H), 1.64 - 1.46 (m, 10H), 1.27 (s, 8H).

2-(10-hydroxydecyl)-2,5,7,8-tetramethylchroman-6-ol (22).

A solution of 21 (3.08 mmol) and freshly prepared 2,3,5-trimethylbenzene-1,4-diol (2.56 mmol) in formic acid (10 ml) was refluxed for 3.5 hours under a nitrogen environment. The reaction mixture was poured onto crushed ice and the organic material was extracted with Et ₂ O under argon. The combined organic phases were washed with H ₂ O, dried over anhydrous MgSO ₄ and concentrated in vacuo. The brown residue was mixed with MeOH and conc. HCl, and the reaction mixture was refluxed under argon for an additional 30 minutes. The solvent was removed in vacuo and the residue was dissolved in Et ₂ O. Washed again with H ₂ O, saturated aqueous NaHCO ₃ , H ₂ O under argon, dried over anhydrous MgSO ₄ , filtered and concentrated to give a brown oil. This was purified by MPLC to obtain compound 22. Yield: 35%; 1H NMR (400 MHz, CDCl3) δ 4.38 (s, 1H), 3.62 (t, J = 6.6 Hz, 2H), 2.59 (t, J = 6.8 Hz, 2H), 2.15 (s, 3H), 2.10 (s , 6H), 1.76 (qq, J = 13.9, 7.1 Hz, 2H), 1.64 - 1.24 (m, 17H), 1.22 (s, 3H).

2-(10-iododecyl)-2,5,7,8-tetramethylchroman-6-ol (23).

에서 주사기를 통해 첨가하였다. 반응 혼합물을 실온에서 추가로 12시간 동안 교반하였다. 혼합물을 Na₂SO₃, H₂O, 염수 용액으로 세척하였고, Na₂SO₄상에서 건조시키고, 여과하고, 감압 하에 농축시켰다. 생성된 잔류물을 MPLC로 정제하여 화합물 23을 얻었다. 1H NMR (400 MHz, CDCl3) δ 4.19 (d, J = 2.7 Hz, 1H), 3.19 (t, J = 4.0 Hz, 2H), 2.60 (t, J = 6.8 Hz, 2H), 2.16 (m, 3H), 2.11 (s, 6H), 1.90 - 1.69 (m, 5H), 1.63 - 1.47 (m, 4H), 1.44 - 1.21 (m, 10H), 0.99 - 0.95 (m, 1H), 0.86-0.83 (m, 3H).To a solution of triphenylphosphine (0.348 mmol), 1H-imidazole (0.348 mmol), and iodine (0.182 mmol) in CH ₂ Cl ₂ (2 ml) was added 22 (0.165 mmol) in CH ₂ Cl ₂ (2 ml). ) to a solution of 0

was added via syringe. The reaction mixture was stirred at room temperature for an additional 12 hours. The mixture was washed with Na ₂ SO ₃ , H ₂ O, brine solution, dried over Na ₂ SO ₄ , filtered and concentrated under reduced pressure. The resulting residue was purified by MPLC to give compound 23. 1H NMR (400 MHz, CDCl3) δ 4.19 (d, J = 2.7 Hz, 1H), 3.19 (t, J = 4.0 Hz, 2H), 2.60 (t, J = 6.8 Hz, 2H), 2.16 (m, 3H) ), 2.11 (s, 6H), 1.90 - 1.69 (m, 5H), 1.63 - 1.47 (m, 4H), 1.44 - 1.21 (m, 10H), 0.99 - 0.95 (m, 1H), 0.86-0.83 (m , 3H).

(10-(6-hydroxy-2,5,7,8-tetramethylchroman-2-yl)decyl)triphenylphosphonium iodide (Mito-VitE; 24).

A mixture of 23 (0.25 mmol) and triphenylphosphine (327 mg, 1.25 mmol) was flushed with argon in a Kimax tube, sealed and stirred as a melt at 90 °C for 48 hours. The crude product was dissolved in CH ₂ Cl ₂ and precipitated from petroleum ether three times. Residual solvent was removed in vacuo to give 24. Yield: 12%; 1H NMR (400 MHz, CDCl3) δ 7.86 - 7.79 (m, 9H), 7.73 - 7.68 (m, 6H), 3.76-3.71 (m, 2H), 2.59 (t, J = 6.8 Hz, 2H), 2.15 ( s, 3H), 2.10 (s, 3H), 2.09 (s, 3H), 1.81-1.72 (m, 2H), 1.63-1.62 (m, 4H), 1.37-1.36 (m, 2H), 1.21-1.18 ( m, 15H); HRMS (ESI, m/z) calculated for C41H52O2P [M]+ 607.3699, found 607.2697.

1-18. SB-U141: Preparation of triphenyl(8-(2,3,4,5-tetramethoxy-6-methylphenyl)octyl)phosphonium

8-Methoxy-8-oxoooctanoic acid (1) was reacted with SOCl ₂ at 50° C. for 4 hours to obtain methyl-8-chloro-8-oxootanoate (2). Methyl 9-oxo-9-(2,3,4,5 _- tetra Methoxy-6-methylphenyl) nonanoate (3) was synthesized. 3 was reacted with LAH and THF at room temperature to synthesize 1-(2,3,4,5-tetramethoxy-6-methylphenyl)octane-1,8-diol (4). 4 was reacted with Pd/C H2 in MeOH to synthesize 8-(2,3,4,5-tetramethoxy-6-methylphenyl)octan-1-ol (5). 5 was reacted with CBr ₄ PPh ₃ in DCM to obtain 1-(8-bromooctyl)-2,3,4,5-tetramethoxy-6-methylbenzene (6). 6 was reacted with PPh ₃ in ACN at 90° C. to obtain triphenyl(8-(2,3,4,5-tetramethoxy-6-methylphenyl)octyl)phosphonium (SB-U141).

1-19. SB-U142: Preparation of triphenyl(12-(2,3,4,5-tetramethoxy-6-methylphenyl)dodecyl)phosphonium

Methyl 12-chloro-12-oxododecanoate (2) was synthesized by reacting 12-methoxy-12-oxododecanoic acid (1) with SOCl ₂ at 50° C. for 4 hours. Methyl 12-oxo-12-(2,3,4,5 _- tetramethoxy-6- Methylphenyl) dodecanoate (3) was obtained. 3 and LAH were reacted in THF to obtain 1-(2,3,4,5-tetramethoxy-6-methylphenyl)dodecane-1,12-diol (4). 4 was reacted with H ₂ , Pd/C and MeOH to obtain 12-(2,3,4,5-tetramethoxy-6-methylphenyl)dodecane-1-ol (5). 5 and CBr ₄ PPh ₃ were reacted in DCM at room temperature to synthesize 1-(12-bromododecyl)-2,3,4,5-tetramethoxy-6-methylbenzene (6). 6 was reacted with PPh ₃ in ACN at 90°C to synthesize triphenyl(12-(2,3,4,5-tetramethoxy-6-methylphenyl)dodecyl)phosphonium (SB-U142).

1-20. SB-U151: Preparation of (8- (4,5-dimethoxy-2-methylphenyl) octyl) triphenylphosphonium

8-Methoxy-8-oxooctanoic acid was reacted with SOCl ₂ at 50° C. to obtain methyl 8-chloro-8-oxooctanoate (2). 2 was reacted with AlCl ₃ in DCM at 40° C. to obtain methyl 8-(4,5-dimethoxy-2-methylphenyl)-8-oxooctanoate (3). 3 was reacted at room temperature in LAH and THF to obtain 1-(4,5-dimethoxy-2-methylphenyl)octane-1,8-diol (4). 4 and Pd/CH ₂ were reacted in MeOH to obtain 8-(4,5-dimethoxy-2-methylphenyl)octan-1-ol (5). 5 was reacted with CBr ₄ PPh ₃ in DCM at room temperature to obtain 1-(8-bromooctyl)-4,5-dimethoxy-2-methylbenzene (6). 6 was reacted with PPh ₃ and ACN at 90° C. to obtain (8-(4,5-dimethoxy-2-methylphenyl)octyl)triphenylphosphonium (SB-U151).

1-21. SB-U152: Preparation of (12-(4,5-dimethoxy-2-methylphenyl)dodecyl)triphenylphosphonium

Methyl 12-chloro-12-oxododecanoate (2) was synthesized by reacting 12-methoxy-12-oxododecanoic acid with SOCl ₂ at 50° C. for 4 hours. 2 was reacted with 1,2-dimethoxy-4-methylbenzene to synthesize methyl 12-(4,5-dimethoxy-2-methylphenyl)-12-oxododecanoate (3). 3 was reacted with LAH to synthesize 1-(4,5-dimethoxy-2-methylphenyl)dodecane-1,12-diol (4). 4 was reacted with Pd/C/H ₂ to synthesize 12-(4,5-dimethoxy-2-methylphenyl)dodecane-1-ol (5). 5 was reacted with CBr ₄ /PPh ₃ to synthesize 1-(12-bromododecyl)-4,5-dimethoxy-2-methylbenzene (6). 6 was reacted with PPh ₃ to synthesize (12-(4,5-dimethoxy-2-methylphenyl)dodecyl)triphenylphosphonium (SB-U152).

Example 2. Recombinant protein production

Genes encoding zTRAP1, hTRAP1 were cloned into a modified pET-Duet vector with an N-terminal hexa-histidine tag followed by a TEV protease cleavage site and expressed in E. coli BL21 (DE3) cells. 15 h after induction with 0.4 mM IPTG at 20 °C, cells were harvested and lysed by sonication. The soluble fraction of the lysate was applied to a Ni2+ affinity chromatography column (GE Healthcare). The hexa-histidine tag was cleaved by TEV protease and the protein was further purified by gel filtration chromatography in a buffer containing 25 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 μM beta-mercaptoethanol (beta-ME).

Example 3. Structural Analysis

The present inventors analyzed the binding structure of TRAP1 and MitoQ in order to derive the structure of a compound that binds to TRAP1.

Purified zTRAP1 was mixed with AMPPNP in a 1:1.5 molar ratio. Crystallization was performed as described in Lavery et al., 2014. After crystal growth, 0.1 mM MitoQ was added to the crystallization drop and incubated for 24 hours. For X-ray diffraction experiments, the crystals were transferred to a well solution containing 20% glycerol and flash frozen in liquid nitrogen. Diffraction data were collected at beamline 5C at the Pohang Accelerator Laboratory (PAL) and processed using HKL-2000 software (Otwinowski and Minor, 1997). The electron density of MitoQ was calculated by the difference Fourier method. Model building and refinement were performed by the Coot and Phenix programs (Adams et al., 2010; Emsley et al., 2010), respectively.

As a result of structural analysis, it was confirmed that the distance between the two protomers of TRAP1 was about 25 Å, and that the distance between the Ub moiety and the TPP moiety had to be appropriate to bind to CBS in TRAP1. Based on this, it was confirmed that a compound structure having an appropriate length is essential for binding to TRAP1 (see FIGS. 1 and 2).

Example 4. Binding force analysis

Fluorescence polarization (FP) analysis

Example 4-1. With SB-TM2 probe

FP buffer (35 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.4 mM KH2PO4 (pH 7.3), 1 mM DTT, 2 mM) containing human full-length TRAP1 protein (400 nM) and SB-TM2 probe (100 nM) MgCl2, 0.1 mg/mL BSA) was added to the material to be analyzed (final volume 100 μl), and reacted at room temperature for 1 hour (96 well plate). FP was measured using a SYNERGY NEO microplate reader by setting an excitation wavelength of 440 nm and an emission wavelength of 500 nm.

As a result, it was confirmed that competitive binding with SB-TM2 was possible from TPP-8, and when the alkyl chain length was greater than C8, it was confirmed that TRAP1 could bind to CBS. In addition, it was confirmed that the binding force increased as the length of the alkyl chain increased. Furthermore, in the comparison of bonding strength with SMX (MitoQ), it was confirmed that the bonding strength of TPP-12, TPP-14, and TPP-16 was superior to that of SMX (MitoQ). A strong binding force was confirmed. (See Figure 4).

In addition, among antioxidants bound to TPP, it was confirmed that TRAP1 binds to CBS when the connection distance between TPP and antioxidant is sufficient (see FIG. 6).

Additionally, it was confirmed that TRAP1 has binding ability to CBS in other synthetic materials linked to TPP and hydrocarbons (see FIG. 8).

Example 4-2. Using PU-H71-FITC

For FP assay, purified recombinant TRAP1 (400 nM) was incubated with the fluorescent probe PU-H71-FITC (10 nM) synthesized as described in Taldone et al., 2013 in the presence of increasing inhibitor concentrations for 2 hours. Fluorescence polarization was measured using a microplate reader (Synergy NEO, BioTek) at room temperature.

As a result, it was confirmed that alkyl-TPP did not compete with PU-H71-FITC (an Hsp90/TRAP1 inhibitor targeting the ATP binding site) and did not bind to the ATP binding site (see FIG. 9b).

In addition, it was confirmed that the TPP-antioxidant conjugate with a long linker does not compete with PU-H71-FITC (an Hsp90/TRAP1 inhibitor targeting the ATP binding site) and does not bind to the ATP binding site ( See Figure 11b).

Example 5. ATPase activity assay

The present inventors conducted an analysis to determine whether the compounds disclosed herein bind to the ATP binding site in TRAP1 and affect the activity of ATPase.

The ATPase activity of TRAP1 was measured using the PiColorLock Gold Phosphate Detection Kit (Abcam). 0.5 μM TRAP1 (wild-type or mutant) was pre-incubated with various concentrations of inhibitors for 30 min, followed by 3 incubation at 37 °C in ATPase activity assay buffer containing 50 mM Tris-HCl, 20 mM KCl and 6 mM MgCl (pH 7.4). Incubated with 0.2 mM ATP for 1 hour. Next, 20 μL of PiColorLock Gold reagent and accelerator mixture (100:1) was added to each sample (100 μL). After 5 min incubation, color development was stopped by adding 10 μL of stabilizer. Absorbance was measured at 620 nm using a microplate reader (Synergy NEO, BioTek). The background signal was normalized by subtracting the absorbance of the unreacted sample.

As a result, in the case of alkyl-TPP, unlike the decrease in ATPase activity with increasing concentration of PU-H71, there was no tendency to decrease ATPase activity in a concentration-dependent manner (see FIG. 9a). This means that alkyl-TPP acts in a different way from PU-H71, which binds to the ATP binding site and inhibits ATPase activity.

Also, in the case of the TPP-antioxidant conjugate and other synthetic materials (SB-U011,014,015), there was no tendency to decrease the ATPase activity in a concentration-dependent manner (see FIGS. 11a and 14).

Example 6. Protein expression analysis

We performed protein expression analysis to determine whether the compounds disclosed herein inhibit TRAP1 or cytoplasmic Hsp90.

Cell culture and treatment

The human cancer cell line 22Rv1 was purchased from the American Type Culture Collection (ATCC) and maintained as recommended by the supplier. Briefly, cancer cells were cultured in DMEM or RPMI medium (GIBCO) containing 10% fetal bovine serum (FBS; ATCC) and 1% penicillin/streptomycin (GIBCO) at 37 °C in a humidified atmosphere of 5% CO2. . Cells were not cultured for more than 6 months.

22Rv1 cells were treated with 5 μM of each compound, cultured for 2 hours, and then analyzed by Western blotting.

antibody

- 액틴 항체는 MP Biomedicals에서 구입하였다. anti-SDHB는 Abcam에서 구입하였다. Anti-Phospho-AMPKα, anti-Cdk4, anti-CHOP and anti-SIRT3 antibodies were purchased from Cell Signaling Technology. Anti-Akt and anti-AMPK were purchased from Santa Cruz Biotechnology. anti-Hsp70 was purchased from BD Biosciences. port -

- Actin antibody was purchased from MP Biomedicals. anti-SDHB was purchased from Abcam.

western blot

Cell lysates were separated by SDS-PAGE and transferred to PVDF membranes. Membranes were blocked with 10% skim milk in TBST (TBS containing 0.05% Tween-20) for 1 hour at room temperature and incubated with primary antibodies overnight at 4 °C. Membranes were washed three times for 1 hour with TBST and incubated with secondary antibody (1:5000) diluted in 10% skim milk in TBST for 1 hour. Membranes were washed three times with TBST and visualized using an enhanced chemiluminescence detection kit (BioRad).

As a result of the experiment, it was confirmed that SDHB and SIRT3 were reduced by inhibiting TRAP1 in the case of TPP-10 to 16, and among them, it was confirmed that TPP-14 and 16 had a strong effect. However, none of the alkyl-TPPs caused changes in the expression of Akt and Cdk4 proteins, nor did they cause changes in Hsp70, which is used as a marker for Hsp90 inhibition (see FIG. 10). It can be confirmed that the alkyl-TPP having a certain length inhibits TRAP1 and does not affect cytoplasmic Hsp90.

In addition, in the case of the TPP-antioxidant conjugate, SDHB and SIRT3 were reduced according to TRAP1 inhibition without inhibition of Hsp90 in the case of the conjugate having a long linker, and in the case of a short linker, protein expression was not affected (see FIG. 12). ). This confirms that only the TPP-conjugate having an appropriate length inhibits TRAP1. Additionally, unlike compounds with a short linker, in the case of a linker with an appropriate length, as a marker for TRAP1 inhibition, it can be confirmed that AMPK is activated (p-AMPK) and mitochondrial unfolded protein response is induced (CHOP) (Fig. 13).

In addition, in the case of other synthetic compounds, it can be confirmed through Western blotting that TRAP1 is inhibited (see FIG. 15).

Example 7. In vivo experiments for confirming the effect of compounds on cancer

Mouse xenografts in vivo.

Immunodeficient athymic nude mice (male, 8 weeks old) were purchased from OrientBio. Mice were maintained in a pathogen-free facility (12-hour dark/light cycle) at the UNIST In Vivo Research Center and fed a standard diet and water. All animal experiments were approved by UNIST (UNISTIACUC-19-11). 22Rv1 cells (1x10 ⁷ ) were subcutaneously injected into both flanks of nude mice. When tumor size reached approximately 100 mm ³ , vehicle (DMSO) and 3 mg/Kg drug (MitoQ, SB-U014 and SB-U015) dissolved in 20% cremophor EL (Sigma) in PBS were administered intraperitoneally daily. Tumor volume was measured daily using an electronic caliper and calculated using the formula: V = 1/2 Х (width) ² Х length. At the end of the experiment, animals were euthanized and tumors were collected for histology and Western blot. Band intensities were quantified using ImageJ software (National Institute of Health, USA). At this time, Western blotting was performed in the same manner as described above.

As a result of the experiment, it was confirmed that when treated with SB-U014,015, the size of the tumor decreased and the weight of the tumor decreased compared to when treated with DMSO (see FIG. 16). In addition, as a result of western blotting, SDHB and SIRT3 were decreased according to TRAP1 inhibition, AMPK was activated (p-AMPK), mitochondrial unfolded protein response was induced (CHOP), and Akt, Cdk4 and Hsp70 were not changed. (See Figure 17).

Through this, it was confirmed that the compound disclosed herein inhibits the growth of cancer cells and reduces the size of cancer cells through TRAP1 inhibition.

Claims (21)

A compound represented by Formula 1 below, or a pharmaceutically acceptable salt thereof:
[Formula 1]

At this time,
L comprises (CH ₂ ) _n ;
Wherein n is an integer of 7 or more and 40 or less,
A is selected from methyl, substituted or unsubstituted aryl, substituted or unsubstituted cycloalkyl, and substituted or unsubstituted heterocyclyl. According to claim 1,
A is substituted with one or more selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxyl C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy; A compound selected from unsubstituted aryl, cycloalkyl and heterocyclyl, or a pharmaceutically acceptable salt thereof. According to claim 2,
A is a substituted or unsubstituted aryl,
The substituted or unsubstituted aryl is selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy A compound which is phenyl substituted or unsubstituted with one or more, or a pharmaceutically acceptable salt thereof. According to claim 3,
The A is

,

,

,

,

,

,

,

,

and

A compound selected from the group consisting of, or a pharmaceutically acceptable salt thereof. According to claim 2,
A is a substituted or unsubstituted aryl,
The substituted or unsubstituted aryl is selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy A compound that is naphthalene substituted or unsubstituted with one or more, or a pharmaceutically acceptable salt thereof. According to claim 5,
The A is

A phosphorus compound, or a pharmaceutically acceptable salt thereof. According to claim 2,
A is a substituted or unsubstituted aryl,
The substituted or unsubstituted aryl is selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy A compound that is benzodioxole substituted or unsubstituted with one or more, or a pharmaceutically acceptable salt thereof. According to claim 7,
The A is

or

A phosphorus compound, or a pharmaceutically acceptable salt thereof. According to claim 2,
A is a substituted or unsubstituted cycloalkyl,
The substituted or unsubstituted cycloalkyl is selected from halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy A compound of cyclohexyl substituted or unsubstituted with one or more selected compounds, or a pharmaceutically acceptable salt thereof. According to claim 9,
A compound wherein A is unsubstituted cyclohexyl, or a pharmaceutically acceptable salt thereof. According to claim 2,
A is a substituted or unsubstituted heterocyclyl,
The substituted or unsubstituted heterocyclyl is halogen, =O, hydroxyl, oxycarbonyl, hydroxy C1-5 alkyl, C1-5 alkyl, C1-5 alkenyl, C1-5 alkynyl and C1-5 alkoxy A pyrrolidine or chroman compound substituted or unsubstituted with one or more selected from, or a pharmaceutically acceptable salt thereof. According to claim 11,
Wherein A is pyrrolidine substituted with one or more selected from oxycarbonyl, C1-5 alkyl and =O, or a pharmaceutically acceptable salt thereof. According to claim 12,
The A is

A phosphorus compound, or a pharmaceutically acceptable salt thereof. According to claim 11,
A compound wherein A is chroman-2-yl substituted with at least one selected from C1-5 alkyl and hydroxyl, or a pharmaceutically acceptable salt thereof. According to claim 14,
The A is

A phosphorus compound, or a pharmaceutically acceptable salt thereof. According to claim 1,
wherein n is an integer of 9 or greater, or a pharmaceutically acceptable salt thereof. According to claim 1,
A TRAP1 inhibitor comprising the compound represented by Formula 1 or a pharmaceutically acceptable salt thereof. According to claim 17,
Wherein the TRAP1 inhibitor binds to CBS. According to claim 1,
A TRAP1-client protein binding inhibitor comprising the compound represented by Formula 1 or a pharmaceutically acceptable salt thereof. According to claim 1,
A pharmaceutical composition for treating cancer comprising a compound represented by Formula 1 or a pharmaceutically acceptable salt thereof. According to claim 1,
A cancer treatment method comprising administering a pharmaceutical composition comprising the compound represented by Formula 1 or a pharmaceutically acceptable salt thereof to a subject in need thereof.

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