KR20220166501A - Pretreatment device and pretreatment method using it - Google Patents

Abstract

The present invention relates to an analyte pretreatment device and a pretreatment method using the same, and more particularly to the analyte pretreatment device and the pretreatment method using the same, allowing a complicated pretreatment process, which is carried out in multiple steps, to be carried out quickly and easily using a single device. The analyte pretreatment device includes a grinding tank, a melting tank, and a lid.

Description

Pretreatment device and pretreatment method using the same {PRETREATMENT DEVICE AND PRETREATMENT METHOD USING IT}

The present invention relates to an analyte pretreatment device and a pretreatment method using the same, and more particularly, to an analyte substance that enables a complicated pretreatment process to proceed quickly and easily using one device, which is carried out in several steps. It relates to a pretreatment device and a pretreatment method using the same.

In general, an analyte contained in a liquid or solid sample is separated and concentrated from the sample through a series of pretreatment processes, and then analyzed using a device such as gas chromatography (GC).

In such a sample preprocessor, a method of volatilizing the analyte included in the sample into a vapor phase, then collecting and concentrating the analyte for a certain period of time is mainly used.

Currently, most laboratories use sample preprocessing devices using methods such as headspace, purge and trap, and SPME (Solid Phase Micro Extraction).

In the headspace method, a certain amount of a sample is put into a container sealed with a diaphragm, and the injected sample is heated to a certain temperature to move volatile components into the space above the sample. In addition, the analyte is directly injected into the analysis device using a syringe or automatically injected into the analysis device through a heated connection tube using a carrier gas. However, the headspace method has a problem in that it takes a very long time to separate the analyte from the sample and is not suitable for analyzing a small amount of the sample.

In the purge-trap method, after putting a liquid or solid sample into a container, an inert gas is blown into the sample to separate volatile components from the sample. Accordingly, the analyte volatilized with the inert gas is collected in the adsorption trap, and when purging for a predetermined time is completed, the adsorption trap is quickly heated to desorb the adsorbed analyte from the adsorption trap. This purge-trap method is suitable for analyzing a small amount of volatile components present in a liquid sample because the volatile components are concentrated in the adsorption trap.

Recently, a pretreatment device using these methods has been commercialized, and a technology for concentrating the analyte contained in the sample without changing the chemical composition of the sample from a small amount of sample of about 1 ml or less is being developed. The preprocessing time is limited to more than 30 minutes.

In addition, such a conventional sample preprocessing device not only takes a lot of time to preprocess the sample due to the complicated preprocessing step, but also has a high possibility of contamination of the sample according to the movement of the sample at each step, so that accurate analysis results cannot be obtained. there is

In addition, there is a problem in that it is not easy to process a large amount of samples and there is difficulty in applying the sensor platform for diagnosis in the field.

The present invention was derived to solve the above problems, and not only can quickly and easily proceed with a pretreatment process that is complicated in several steps using one device, but also materials according to the movement of the sample at each step. Its object is to provide an analyte pretreatment device that enables accurate analysis of a sample by significantly reducing the possibility of contamination.

Another object of the present invention is to provide a method for pre-processing an analyte using the analyte pre-processing device.

The present invention may also have as its object to achieve other objects that can be easily derived by a person of ordinary skill in the art from these objects and the overall description of this specification in addition to the above clear objects.

In order to achieve the above object, the analyte preprocessor of the present invention,

A crushing tank having a crushing hole for crushing samples on the upper surface of the bottom, having a side wall at the edge, and an open upper surface;

A dissolution tank having a cylindrical shape with open upper and lower surfaces, the lower surface being fastened to the upper surface of the grinding tank, and accommodating a solvent for dissolving the analyte from the sample; and

The lower surface of the lid is opened and the lower surface is fastened with the upper surface of the dissolution tank.

It is characterized in that it includes.

In addition, the lateral cross-section of the crusher may be a semicircle, an arc, a triangle, a rectangle, or a combination thereof.

And, the grinding tank may be provided with a plurality of grinding holes.

And, the height of the grinding hole may be 0.1 to 50 mm, 1 to 40 mm, 2.5 to 30 mm, or 5 to 20 mm.

In addition, each edge of the upper surface of the crushing tank and the lower surface of the melting tank may be fastened by screwing.

In addition, each edge of the upper surface of the dissolution tank and the lower surface of the lid may be fastened by screwing.

In addition, the lid may have a discharge port formed on an upper side for discharging a solution in which the analyte is dissolved, and a stopper closing the lid.

In addition, a disc-shaped filter for filtering a solution in which the analyte is dissolved from the sample may be further included.

The filter may be seated on the lower filter fixing part formed on the inner circumferential surface of the top of the melting tank and fixed by the upper filter fixing part formed on the inner circumferential surface of the lower end of the lid.

Also,

The crushing tank may further include a disc-shaped pressure cap having a plurality of through holes.

In addition, in the pressure cap, a guide perpendicular to the disk may be formed along an edge of the disk.

In addition, the pressure cap may have a plurality of protrusions formed on an upper surface, a lower surface, or an upper and lower surface of the disc.

In addition, the protrusion may be radially formed from the center of the press cap disk.

On the other hand, the analyte pretreatment method of the present invention

(A) accommodating the sample to be analyzed in the crushing tank;

(B) crushing the sample by applying pressure, shear force, or pressure and shear force to the sample;

(C) fastening the lower surface of the dissolution tank and the upper surface of the crushing tank;

(D) supplying a solvent through the open upper surface of the dissolution tank;

(E) fastening the lower surface of the lid and the upper surface of the dissolution tank;

(F) stirring the sample to dissolve the analyte in the solvent; and

(G) recovering a solution in which the analyte is dissolved

It is characterized in that it includes.

In addition, in the step (B), the pressure cap may be removed after covering and pulverizing the pressure cap on the sample.

In addition, after step (B) and before step (E),

A step of covering the upper surface of the dissolution tank with a filter may be further included.

And, at the time of dissolution in step (F), vibration, ultrasonic waves, or vibration and ultrasonic waves may be applied.

In the step (G), the solution may be recovered through the outlet.

According to the problem solving means of the present invention as described above, various effects including the following can be expected. However, the present invention is not established when all of the following effects are exerted.

The present invention not only enables the pretreatment process, which is complicated in several steps, to be carried out quickly and easily using one device, but also significantly reduces the possibility of contamination of materials due to the movement of the sample at each stage, thereby It has the effect of enabling an accurate analysis of .

In addition, the efficiency of dissolution into a solvent is improved by crushing a large amount, especially a sample, so that a sufficient amount of the analyte can be recovered even in a small amount of sample, and the structure is simple and easy to operate, so it is easy to use as a sensor platform for diagnosis in the field. There is an effect that can be applied appropriately.

1 is a perspective view showing the structure of an analyte preprocessing apparatus according to an embodiment of the present invention.
Figure 2 is a side cross-sectional view showing a state in which the filter according to an embodiment of the present invention is fixed between the lid and the melting tank.

Hereinafter, preferred embodiments of the present invention will be described in detail.

However, the following is only a detailed description by exemplifying specific embodiments, and since the present invention may be variously changed and may have various forms, the present invention is not limited to the specific embodiments illustrated. It should be understood that the present invention includes all modifications, equivalents and substitutes included in the spirit and scope of the present invention.

In addition, in the following description, many specific details such as specific components are described, which are provided to help a more general understanding of the present invention, and it is common in the art that the present invention can be practiced without these specific details. It will be self-evident to those who have the knowledge of And, in describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description will be omitted.

And, the terms used in this application are only used to describe specific embodiments, and are not intended to limit the present invention. Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the present application, they should not be interpreted in an ideal or excessively formal meaning. don't

In this application, expressions in the singular number include plural expressions unless the context clearly dictates otherwise.

In this application, terms such as first and second may be used to describe various components, but the components should not be limited by the terms. These terms are only used for the purpose of distinguishing one component from another. For example, a first element may be termed a second element, and similarly, a second element may be termed a first element, without departing from the scope of the present invention.

In this application, terms such as 'comprise', 'include' or 'having' are intended to indicate that the features, components (or components), etc. described in the specification exist, but one or more other features or It does not mean that components or the like do not exist or cannot be added.

1 is a perspective view showing the structure of an analyte preprocessing device according to an embodiment of the present invention, and FIG. 2 is a side view showing a state in which a filter according to an embodiment of the present invention is fixed between a lid and a dissolution tank. it is a cross section

As shown in these drawings, the analyte preprocessing apparatus according to the present invention includes a crushing tank 100 having a crushing hole 110 for crushing a sample on the upper surface of the bottom, having a side wall at the edge, and having an open upper surface. , Dissolution tank 200 having a cylindrical shape with open upper and lower surfaces, the lower surface being fastened to the upper surface of the pulverization tank 100, and accommodating a solvent for dissolving the analyte from the sample, and the lower surface is open , It is characterized in that the lower surface comprises a lid 400 fastened to the upper surface of the dissolution tank 200.

As shown in FIG. 1, the crushing tank 100 is a short cylindrical member having a constant internal space so that the sample can be accommodated by introducing the sample into the inside through the open upper surface, and the sample is placed on the upper surface of the bottom. A crushing hole 110 for crushing is installed.

When crushing the sample by applying pressure, shear force, or pressure and shear force to the sample accommodated in the crushing tank 100, the crushing hole 110 provides an obstacle or a piercing or tearing point to promote the crushing of the sample. As a result, the dissolution efficiency is increased by increasing the contact area between the sample and the solvent in the subsequent dissolution step. The side section, which is a cross section of the grinding hole 110 performing this function, viewed from the side, has a height from the top of the bottom of the grinding tank 100 of 0.1 to 50 mm, 1 to 40 mm, 2.5 to 30 mm, or 5 to 5 mm. It may be a half circle of 20 mm, an arc, a triangle, a rectangle, or a combination thereof. In the present invention, the grinding tank 100 may be provided with a plurality of grinding holes 110 as shown in FIG.

A screw thread is formed on the upper edge of the grinding tank 100, that is, on the inner or outer circumferential surface, and can be fastened with a screw thread formed on the lower edge, ie, on the inner or outer circumferential surface of the melting tank 200 to be described later.

As shown in FIG. 1, the upper surface of the dissolution tank 200 fastened in this way is open to supply a solvent for dissolving the analyte from the sample, and the supplied solvent can be transferred to the grinding tank 100. It is also open.

As shown in FIG. 2, a screw thread is formed on the upper edge of the dissolution tank 200, that is, on the inner or outer circumferential surface, and can be fastened with a screw thread formed on the lower edge of the lid 400, that is, on the inner or outer circumferential surface, which will be described later. there is.

As such, when the lid 400, the dissolving tank 200, and the grinding tank 100 are fastened by screwing, the sample and the solvent accommodated inside are separated from the outside, and the sample is analyzed by the solvent from the sample in a state in which contamination is prevented. Dissolution of the substance may occur.

The lid 400 may be opened to recover the solution after dissolution of the analyte is completed. Alternatively, an outlet 450 through which the solution is discharged may be formed at an upper side, for example, at an edge or at the center of the upper side as shown in FIG. 1, and may be provided with a stopper 455 closing the outlet.

As shown in FIGS. 1 and 2 , the analyte preprocessing apparatus of the present invention may further include a disc-shaped filter 300 for filtering a solution in which the analyte is dissolved from a sample. Various analytical equipment used these days are very sensitive, and in most cases, they do not perform well even with small impurities. The pretreatment method of the present invention goes through the step of pulverizing the sample, in which case there is a risk that the pulverized sample debris is delivered to the analysis equipment. It is preferable to have a filter 300 to prevent this.

As shown in FIG. 2, the filter 300 is seated on the lower filter fixing part 310 formed on the upper inner circumferential surface of the dissolution tank 200, and then, when the dissolution tank 200 and the lid 400 are screwed together, the lid (400) It can be fixed by the upper filter fixing part 320 formed on the lower inner circumferential surface.

As shown in FIG. 1 , the crushing tank 100 of the preprocessing apparatus for the analyte of the present invention may further include a disk-shaped pressure cap 150 having a plurality of through holes 152 formed therein. In order to pulverize the sample accommodated in the pulverization tank 100, an external force such as pressure, shear force, or pressure and shear force is applied. In order to efficiently transfer this external force to the sample, a pressure cap 150 covering the sample can be used.

As shown in FIG. 1 , the pressure cap 150 may have a plurality of protrusions formed on the upper surface, the lower surface, or the upper and lower surfaces of the disc. Among the protrusions, the upper protrusion 156 ensures that the external force is sufficiently transmitted to the pressure cap 150 without idling, and the lower protrusion 154 fixes the sample so that the external force transmitted to the pressure cap 150 is sufficiently transmitted to the sample. to be conveyed As shown in FIG. 1 , the upper protrusion 156 and the lower protrusion 154 may be radially formed from the center of the disk of the pressing cap 150 .

In addition, as shown in FIG. 1 , the pressure cap 150 may have a guide 158 perpendicular to the disk formed along the edge of the disk. Through this guide 158, the pressure cap 150 can be moved up and down in the grinding tank 100 while maintaining a level, and as a result, the external force can be evenly transmitted to the entire area of the grinding tank 100.

The analyte pretreatment method using the analyte preprocessor according to an embodiment of the present invention having such a configuration includes the following steps:

(A) accommodating the sample to be analyzed in the crushing tank 100;

(B) crushing the sample by applying pressure, shear force, or pressure and shear force to the sample;

(C) fastening the lower surface of the dissolution tank 200 and the upper surface of the crushing tank 100;

(D) supplying a solvent through the open upper surface of the dissolution tank 200;

(E) fastening the lower surface of the lid 400 and the upper surface of the dissolution tank 200;

(F) stirring the sample to dissolve the analyte in the solvent; and

(G) recovering a solution in which the analyte is dissolved.

First, a sample to be analyzed is accommodated in the crushing tank 100, and the sample is evenly distributed over the entire bottom of the crushing tank 100.

Subsequently, the sample is pulverized by applying pressure, shear force, or pressure and shear force to the sample. At this time, the external force such as the pressure, shear force, or pressure and shear force may be provided by a hand wearing sanitary gloves or surgical gloves, but it is more preferable to apply an external force to the sample by covering the pressure cap 150 described above.

When the crushing is completed, the pressure cap 150 is removed, and as shown in FIG. 1, the lower surface of the dissolution tank 200 and the upper surface of the crushing tank 100 are coupled. Subsequently, a solvent is supplied through the open upper surface of the dissolution tank 200 so that the sample is immersed in the solvent so as to be dissolved.

After the solvent is supplied, as shown in FIG. 1, the open upper surface of the dissolution tank 200 is covered with a lid 400 to block it from the outside. The lid 400 is preferably fastened to the dissolution tank 200 through screw coupling, and through this, the inside of the dissolution tank 200 and the crushing tank 100 is blocked from the outside to prevent contamination.

Furthermore, it is more preferable to cover the upper surface of the dissolution tank 200 with a filter 300 before covering it with the lid 400 as shown in FIGS. 1 and 2 .

The sample inside the analyte preprocessor of the present invention, which is composed of the cap 400, the dissolution tank 200, and the crushing tank 100, is dissolved by a solvent, and it is preferable to stir to increase the dissolution efficiency. The agitation is generally performed by shaking the analyte preprocessor of the present invention up and down a predetermined number of times, but rotational agitation may be performed by magnetic force by inserting a sterilized magnetic bar after pulverizing the sample. In addition, it is possible to apply vibration, ultrasonic waves, or vibration and ultrasonic waves to improve dissolution efficiency.

When the analyte in the sample is dissolved in the solvent, the solution in which the analyte is dissolved is recovered and injected into the analysis device. The collection may be performed by opening the cover 400, but as shown in FIG. 1, when the filter 300 is provided, open the stopper 455 and turn the analyte preprocessing device of the present invention over to discharge it from the outlet 450. It is more preferable to recover the solution to be.

Although the preferred embodiments of the present invention have been described above, the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various modifications without departing from the gist of the present invention. Of course it is possible. Therefore, the scope of the present invention should not be construed as being limited to the above embodiments, and should be defined by not only the claims to be described later, but also those equivalent to these claims.

100: grinding tank 110: grinding hole
150: pressurized cap 152: through hole
154: lower protrusion 156: upper protrusion
158: guide 200: melting tank
300: filter 310: lower filter fixing part
320: upper filter fixing part 400: lid
450: outlet 455: stopper

Claims (6)

A crushing tank having a crushing hole for crushing samples on the upper surface of the bottom, having a side wall at the edge, and an open upper surface;
A dissolution tank having a cylindrical shape with open upper and lower surfaces, the lower surface being fastened to the upper surface of the pulverization tank, and accommodating a solvent for dissolving the analyte from the sample; and
The lower surface of the lid is opened and the lower surface is fastened with the upper surface of the dissolution tank.
Including, analyte pre-processing device. The method of claim 1,
The analyte preprocessor, characterized in that it further comprises a disc-shaped filter for filtering the solution in which the analyte is dissolved from the sample. The method of claim 1,
The pulverization tank is characterized in that it further comprises a pressure cap in the form of a disk formed with a plurality of through-holes, the analyte pre-processing device. (A) accommodating the sample to be analyzed in the crushing tank;
(B) crushing the sample by applying pressure, shear force, or pressure and shear force to the sample;
(C) fastening the lower surface of the dissolution tank and the upper surface of the crushing tank;
(D) supplying a solvent through the open upper surface of the dissolution tank;
(E) fastening the lower surface of the lid and the upper surface of the dissolution tank;
(F) stirring the sample to dissolve the analyte in the solvent; and
(G) recovering a solution in which the analyte is dissolved
Including, the analyte pretreatment method. The method of claim 4,
In the step (B), the analyte pretreatment method is characterized in that the pressure cap is covered on the sample and pulverized, and then the pressure cap is removed. The method of claim 4,
After step (B) and before step (E),
Characterized in that it further comprises the step of covering the upper surface of the dissolution tank with a filter, the analyte pretreatment method.

Images