KR20220143008A - Cover for applying an electric field and an electric field applying device including the same - Google Patents

Abstract

The cover for applying an electric field according to the present embodiment includes an electrode for applying a voltage in each accommodation space. The electrode of this embodiment includes a positive electrode part, a negative electrode part spaced apart from the positive electrode part, and an insulating plate for insulating the positive electrode part and the negative electrode part, and the positive electrode part is a column ( a plurality of positive connectors provided for each column), a first resistor provided in the positive connector, and a second resistor connecting adjacent positive connectors among the positive connectors in series.

Description

Cover for applying an electric field and an electric field applying device including the same

The present invention relates to a cover for applying an electric field capable of efficiently generating an electric field in a multi-well plate, and an electric field applying device including the same.

In research in biochemistry and pharmaceuticals, a plastic container called a multi-well plate or multi-well plate is widely used to divide a small amount of sample and conduct an experiment in which an electric field is applied to each sample. .

Experiments for applying an electric field may include electrofusion, electrostimulation, and electroporation.

The perforated plate as described above can perform high-throughput analysis or screening of the sample samples by testing the maximum sample sample in the shortest time. Accordingly, various types of perforated plates having 24, 96, 384, etc. accommodation spaces in which specimens can be accommodated are being commercialized.

Korean Patent No. 1077008 (March 15, 2004. Priority date) discloses chambers each including at least a pair of electrodes each having a first electrode and a second electrode in order to apply a voltage to generate an electric field in one chamber. A container equipped with it is disclosed.

U.S. Patent No. 6878539 (applied on Oct. 28, 2003) discloses an electrode structure for applying electrical stimulation to a perforated plate, in which the electrode is positioned under each space in which the sample is accommodated.

According to the above technique, since the electrode is provided in the lower part of each space in which the sample is accommodated in the perforated plate, the perforated plate can only be used for one-time use for sample analysis. There is a problem with this increasing.

In addition, it is difficult to apply electrical stimulation of the same condition to different samples at once or to apply electrical stimulation under different conditions to the same sample at once because the current is selectively supplied instead of to the electrodes provided in each of the perforated plates at once. , there is a problem in that it is not possible to find an optimal electrical stimulation condition.

The present invention has been devised to solve the problems of the prior art, and it is possible to efficiently generate an electric field in a perforated plate, and to provide a cover for applying an electric field that can be used semi-permanently, and an electric field applying device including the same. There is this.

It is an object of the present invention to provide an electric field applying device for applying different voltages to each of the perforated plates provided with the accommodation space using unit connectors respectively positioned on the accommodation space.

The cover for applying an electric field according to this embodiment includes: a case provided to be coupled to a multi-well plate having a plurality of accommodating spaces in which a sample can be accommodated; and an electrode provided on the lower side of the case and applying a voltage to each of the accommodating spaces when the case is coupled on the perforated plate.

The electrode is located under the case, the anode electrode portion is provided with a plurality of anodes respectively positioned inside the accommodation spaces, the both sides of the electrode portion is located under the a plurality of holes through which the anode is provided. It may include an insulating plate and a cathode positioned below the insulating plate and provided with a plurality of cathodes positioned on inner peripheral surfaces of the accommodating spaces to be spaced apart from the anodes, respectively.

In addition, the electrode may include a positive electrode part, a negative electrode part spaced apart from the positive electrode part, and an insulating plate that insulates the positive electrode part from the negative electrode part.

The positive electrode part may include a plurality of positive connectors provided for each column in which the accommodating spaces are arranged, a first resistor provided in the positive connector, and a second resistor connecting adjacent positive connectors among the positive connectors in series. can

Each of the plurality of positive connectors may include a plurality of unit connectors positioned above the accommodating space, and each of the plurality of unit connectors may be provided with a positive pole accommodated in the accommodating space.

The first resistor may include a resistor connecting adjacent unit connectors among the plurality of unit connectors.

The first resistors may be arranged in a line while being spaced apart from each other in a first direction, and the second resistors may be arranged in a line while being spaced apart from each other in a second direction crossing the first direction.

The positive electrode part may include a positive electrode body provided with a positive electrode terminal 223 and the plurality of unit connectors. The positive electrode part may further include a PCB provided with a first switch connected in parallel with the first resistor and a second switch connected in parallel with the second resistor.

The PCB may be provided with a plurality of through-holes through which the anode passes.

In addition, each of the plurality of through-holes provided in the PCB may correspond to the anode. In addition, the plurality of through-holes provided in the PCB substrate may correspond to each of the plurality of holes provided in the insulating plate.

The anodes may be configured in a pin shape that is positioned one at a time at the center of the accommodating spaces.

The cathodes may be positioned one at a time on the inner circumferential surfaces of the accommodating spaces, and may have a cylindrical shape corresponding to the inner circumferential surfaces of the accommodating spaces.

The cathodes may be configured in a pin shape that is respectively positioned at least two or more along the inner circumferential surface of the accommodation spaces.

The cathodes may be configured in the form of pogo pins.

The lower ends of the anodes and the cathodes may be positioned at the same height as each other.

The lower ends of the anodes and the cathodes may be supported on inner bottom surfaces of the accommodating spaces made of a non-conductive material.

The anodes and cathodes may be plated with one of high-conductivity metals harmless to the human body.

The positive electrode part may further include a positive electrode terminal connected to the positive electrodes and protruding outwardly of the case.

The negative electrode part may further include a negative terminal connected to the negative electrodes and protruding outward of the case at a position spaced apart from the positive terminal.

The positive electrode part may include a plurality of positive connectors connecting the positive electrodes for each column in which the receiving spaces are arranged, and a plurality of resistors connecting the positive electrodes in series between the positive connectors.

The positive electrode unit may include at least one switch connected in parallel with at least one of the resistors and connected in series between the positive connectors.

The case and the electrode may be provided with air outlets at positions corresponding to the accommodation spaces, respectively.

The electric field applying device according to the present embodiment includes: a multi-well plate having a plurality of accommodating spaces in which a sample can be accommodated; and a cover for applying an electric field which is detachably provided on the perforated plate and applies a voltage in each accommodation space when coupled on the perforated plate.

When the cover for applying an electric field of this embodiment is mounted on the perforated plate, the electrode on the cover side is positioned inside each of the plurality of accommodating spaces of the perforated plate, so that an electric field can be independently formed in each accommodating space.

Therefore, it is possible to efficiently generate an electric field in the disposable perforated plate by using the semi-permanent cover, and it is possible to reduce the cost of testing for sample analysis.

In addition, by integrally manufacturing the positive connectors and the positive electrodes or electrically coupling them through a common power source, the voltage variable electrode provided on the cover side can be simply configured.

Furthermore, by controlling the switch between the unit connectors, different electric fields can be implemented in each of a plurality of receiving spaces, so that electric stimulation of the same condition is applied to different samples at once, or electric stimulation of different conditions is applied to the same sample at once. and, thereby, it is possible to easily and quickly find the optimal electrical stimulation conditions.

1 is a schematic diagram showing an electric field application device of the present invention.
Figure 2 is a view showing a state in which the cover of Figure 1 is coupled to the perforated plate.
Figure 3 is an exploded perspective view showing an embodiment of the present invention.
4 is a perspective view of the anode electrode shown in FIG. 3;
5 is a cross-sectional view showing an embodiment of the present invention;
6 is a cross-sectional view taken along line AA shown in FIG. 5;
7 is a cross-sectional view taken along line BB shown in FIG. 5;
8 is an enlarged view of an anode electrode part and an insulating plate according to an embodiment of the present invention;
9 is a partially cut-away perspective view of an electric field application device according to an embodiment of the present invention;
10 is a diagram showing a first resistor and a second resistor according to an embodiment of the present invention;
11 is a diagram showing voltages applied to a plurality of accommodation spaces by the resistors shown in FIG. 10;

Figure 1 is a schematic view showing the electric field applying device of the present invention, Figure 2 is a view showing a state in which the cover of Figure 1 is coupled to the perforated plate.

According to the electric field application device of the present invention, a multi-well plate (100) provided with a plurality of accommodating spaces (W) in which samples are accommodated, and a perforated plate (100) detachably provided on the perforated plate (100) It may include a cover 200 for applying an electric field to each accommodation space (not shown) of the , and a control unit 300 for controlling the voltage supplied to the electrode E on the cover 200 side.

The perforated plate 100 is made of a plastic material so that a plurality of accommodating spaces (W) for accommodating samples are provided, and 6-well, 12-well, 24-well, It can be manufactured in 48-well, 96-well, etc.

While the perforated plate 100 is manufactured for one-time use, the cover 200 is manufactured to be used semi-permanently, and the cover 200 can be detached from the commercially available perforated plate 100 .

The cover 200 is provided with a plurality of electrodes E at positions corresponding to the respective receiving spaces W of the perforated plate 100 , and when the cover 200 is coupled to the perforated plate 100 , the cover 200 . The side electrodes (E) may be located inside each accommodation space (W) of the perforated plate (100). A detailed configuration of the cover 200 will be described in detail below.

The control unit 300 may be connected to the electrodes E on the cover 200 side, and supply a voltage to the electrodes E on the cover 200 side to control the electric field formed inside each accommodation space W. have.

3 to 5 are views illustrating an electric field applying device according to an embodiment of the present invention, FIG. 6 is a cross-sectional view taken along line A-A shown in FIG. 5, and FIG. 7 is a cross-sectional view taken along line B-B shown in FIG.

The electric field application device of the present disclosure includes a perforated plate 100 and a cover 200 detachably provided on the perforated plate 100 .

The perforated plate 100 has a rectangular plate shape, and may be made of plastic, which is a non-conductive material, and each receiving space W may be provided in the form of a cylindrical chamber with a predetermined interval on the upper surface thereof.

The upper part 110 of the perforated plate may be formed to be stepped in a smaller shape than the lower part 120 of the perforated plate, and the case 212 of the cover may be fitted to the upper part 110 of the perforated plate. That is, the lower portion 120 of the perforated plate has a large square plate shape, while the upper portion 110 of the perforated plate may be configured in a smaller square plate shape.

When the upper part 110 of the perforated plate is viewed from the upper side, two inclined surfaces 111 and 112 from which corner portions are cut may be provided, and the two inclined surfaces 111 and 112 outside the positive terminal 223 to be described below. and the negative terminal 243 may be exposed.

Since the interval between each accommodation space (W) is configured to be smaller than the diameter of each accommodation space (W), the number of each accommodation space (W) can be large compared to the limited size of the perforated plate (100).

Of course, the shape, size, number, and arrangement of each accommodating space W may be variously configured, but is not limited thereto.

The anodes 222 and the cathodes 242 to be described below are spaced apart from each other inside each receiving space W, but may come into contact with the bottom surface of each receiving space W, so they are non-conductive materials and have a predetermined elasticity. A pad-shaped support part 121 with a may be additionally installed on the bottom surface of each accommodation space (W), but is not limited thereto.

The cover 200 includes an electrode capable of generating an electric field in each receiving space (W) of the case 210, which can be fitted to the upper part 110 of the perforated plate, and the perforated plate 100 coupled to the lower side of the case 210 ( E) may consist of

The case 210 may be made of a non-conductive material like the perforated plate 100 in the form of a cover having a rectangular upper surface and four side surfaces. Since the case 210 does not come in direct contact with cells and drugs, it may be made of one of various materials such as polypropylene, polycarbonate, ABC, etc., which are easy to use, and is not limited thereto.

The case 210 is configured to be at least larger than the upper part 110 of the perforated plate, but when the case 210 is coupled to the upper part 110 of the perforated plate, the inner surface of the case 210 is outside the upper part 110 of the perforated plate. It may come into contact with the side, and the position of the electrode coupled to the lower side of the case 210 may be limited inside each accommodation space W of the perforated plate 100 .

Like the upper part 110 of the perforated plate, the case 210 may be provided with two inclined surfaces 211 and 212 from which two corners are cut when the case 210 is viewed from the upper side, and the two inclined surfaces 211 and 212 may be provided. ), the positive terminal 223 and the negative terminal 243 to be described below may be exposed to the outside.

The case 210 is provided with a plurality of air discharge holes 210h at positions corresponding to the respective accommodation spaces W of the perforated plate 100, so that even if the case 210 is coupled to the upper portion of the perforated plate 100 The air remaining in each receiving space (W) of the stencil 100 is allowed to escape to the outside.

The electrode E may include a positive electrode part 220 made of a conductive material, an insulating plate 230 made of a non-conductive material, and a negative electrode part 240 made of a conductive material.

Specifically, the electrode E includes a negative electrode part 240 spaced apart from the positive electrode part 220 and an insulating plate 230 that insulates the positive electrode part 220 from the negative electrode part 240 . can do. The anode electrode part 220 and the cathode electrode part 240 may be made of at least one of gold, platinum, and stainless steel, or made of a conductive material, and then plated with gold or platinum that can ensure biocompatibility.

On the other hand, the insulating plate 230 is made of a non-conductive material, and may be made of polystyrene, polypropylene, glass, quartz, etc. that does not affect the electric field and does not harm cells and drugs.

The positive electrode unit 220 may be provided with a positive electrode body 221 located on the lower side of the upper surface of the case 210 , and a plurality of positive electrodes 222 provided in the form of pins downwardly on the positive electrode body 221 , The body 221 may be provided with a positive electrode terminal 223 connected to one end of the positive electrode body 221 so as to be exposed to the outside of one side inclined surface 211 of the case 210 .

In this case, the positive electrode body 221 may include a plurality of positive connectors A1 to A6 included in the positive electrode part 220 . In addition, the positive electrode part 220 may include a plate shape formed by combining the PCB 250 and the positive electrode body 221 .

In the drawings of the present disclosure, the plurality of positive connectors are expressed as A1 to A6, but this is only an example and the plurality of positive connectors is not limited to A1 to A6.

The positive electrode body 221 has the same shape as the upper surface of the case 210 , and may be configured to be molded to the lower surface of the upper surface of the case 210 , and has a plurality of positions corresponding to the air discharge holes 210h of the case 210 . Air discharge holes 221h may be provided.

The anodes 222 are in the form of pins that can be accommodated in each accommodating space W of the perforated plate 100 , and may be provided at positions corresponding to the center of each accommodating space W of the perforated plate 100 . . The anodes 222 may be arranged in a lattice form at a predetermined interval like the arrangement of each accommodation space W, but is not limited thereto.

The positive electrode terminal 223 is a portion bent downwardly on one side of the positive electrode body 221 and then horizontally bent outward, exposed on one side of the inclined surface 211 of the case 210 , below the upper surface of the perforated plate 100 . / It can be seated in the stepped portion between the lower part (110, 120). As the positive terminal 223 is electrically connected to the controller 300 (shown in FIG. 1 ), an externally supplied voltage may be applied thereto.

The insulating plate 230 may include a main insulating part 231 having the same plate shape as the anode body 221 , and an auxiliary insulating part 232 bent downward at one side of the main insulating part 231 . The main insulating part 231 may be mounted on the lower side of the positive electrode body 221 , and the auxiliary insulating part 232 may insulate a vertical portion of the positive electrode terminal 223 from the negative electrode part 240 .

Similarly, the insulating plate 230 may be provided with a plurality of air exhaust holes 231h at positions corresponding to the air exhaust holes 210h of the case 210 and the air exhaust holes 221h of the positive electrode body 221 . can

In addition, the insulating plate 230 has a plurality of anodes 222 through which the anodes 222 can penetrate at positions corresponding to the anodes 222 , that is, at positions corresponding to the center of each accommodation space W of the perforated plate 100 . Holes 230H may be provided, and each hole 230H may be located at one side of each air exhaust hole 231h.

The negative electrode part 240 includes a plate-shaped negative electrode connection part 241 coupled to the lower side of the insulating plate 230, and a plurality of negative electrodes 242 provided in a cylindrical shape protruding downward to penetrate the negative electrode connection part 241, The case 210 may include a negative terminal 243 connected to one end of the negative connection part 241 so as to be exposed to the outside of the other inclined surface 212 of the case 210 .

The negative electrode connection part 241 has the same shape as the lower surface of the insulating plate 230 and may be configured to be molded to the lower surface of the upper surface of the case 210 .

The negative electrodes 242 have a cylindrical shape that can be accommodated on the inner circumferential surface of each accommodation space W of the perforated plate 100 , and may be provided to extend downwardly through the negative electrode connection part 241 . Of course, each of the air discharge holes 210h , 221h , and 231h provided in the case 210 , the positive electrode part 220 , and the insulating plate 230 may be located inside the negative electrodes 242 .

The negative terminal 243 is a portion bent downwardly on the other side of the negative electrode connection part 241 and then horizontally bent outward, exposed below the other side inclined surface 212 of the case 210 , and of the perforated plate 100 . It may be seated in a stepped portion between the upper/lower portions 110 and 120 . The negative terminal 243 may be externally grounded.

When the cover 200 according to the first embodiment configured as described above is coupled to the perforated plate 100 , the positive electrodes 222 and the negative electrodes 242 are each containing the same type or different types of samples in each receiving space W ), and the control unit 300 may supply a voltage through the positive terminal 223 .

Accordingly, an electric field is uniformly formed from each positive electrode 222 to each negative electrode 242 surrounding it, and the cells and chemical processes included in the sample can obtain uniform results by the uniform electric field.

Meanwhile, the cover 200 of the first embodiment configured as described above is separated into a case 210 , a positive electrode unit 220 , an insulating plate 230 , and a negative electrode unit 240 , respectively, and then washed and sterilized It can be re-assembled later to make it reusable.

In addition, the negative electrode part 240 operating in the ground form can be assembled or integrated into the perforated plate 100, and the polarity or shape/ The shape can be changed, and the cover 200 can be manufactured in various sizes and shapes with a structure that can be coupled to various types of perforated plates 100 that have already been commercialized, but is not limited thereto.

The device for applying an electric field of the present disclosure may be configured in the form of a pogp pin in which the cathodes 242 are disposed along the inner circumferential surface of each accommodation space W at a predetermined interval.

In detail, the plate-shaped negative electrode connection part 241 is provided with holes having the same size as each accommodation space (W) at positions corresponding to each accommodation space (W), and the negative electrodes 242 in the form of a pogo pin are connected to the negative electrode connection part. It may be provided to protrude downward at a predetermined interval in the circumferential direction along each hole of 241 .

The anodes 242 in the form of pogo pins are made of stainless steel or gold-plated, and commercially available products can be applied as they are.

Of course, even if the anodes 242 are configured in a pogo pin type, the anodes 222 are positioned at the center of each accommodation space W, and the cathodes 242 are spaced apart from each other along the inner circumferential surface of each accommodation space W. is positioned, it is possible to form a uniform electric field inside each accommodation space (W).

In addition, when the negative electrodes 242 are configured in a pogo pin type, a predetermined restoring force can be provided even if the negative electrodes 242 come in contact with the bottom surface of each accommodation space (W) side, so the floor on the side of each accommodation space (W) It may be applicable even if the surface is not flat.

In addition, even if some of the negative electrodes 242 are damaged, only the damaged pogo pin can be replaced and used, so maintenance and repair are easy, and cost can be reduced.

In the electric field applying device of the present disclosure, an electric circuit connecting the anodes 222 to form a different electric field in each accommodation space W may be configured differently.

In detail, a plurality of positive connectors A1 to A6 connecting the positive electrodes 222 for each column in which each receiving space W is disposed, and a first resistor provided in the positive connectors A1 to A6 (R1) and a second resistor R2 connecting adjacent positive connectors among the plurality of positive connectors in series. In this case, the first resistor R1 may include a resistor connecting adjacent unit connectors among the plurality of unit connectors C1 to C4.

In the drawings of the present disclosure, the plurality of unit connectors are expressed as C 1 to C4, but this is only an example and the plurality of unit connectors is not limited to C1 to C4.

And each of the plurality of positive connectors A1 to A6 includes a plurality of unit connectors C1 to C4 positioned above the accommodation space, and each of the plurality of unit connectors includes a positive electrode 222 accommodated in the accommodation space. can be provided.

The positive electrode unit 220 of the present disclosure may include a positive electrode body 221 provided with a positive electrode terminal 223 and the plurality of unit connectors C1 to C4, and the positive electrode unit 220 includes a first resistor ( The PCB 250 may further include a first switch S1 connected in parallel with R1 and a second switch S2 connected in parallel with the second resistor R2.

In this case, the PCB 250 may be provided with a plurality of through holes 251h through which a plurality of anodes are respectively penetrated. Each of the plurality of through-holes 251h provided in the PCB 250 may correspond to each of the plurality of anodes 222 . In addition, the plurality of through-holes 251h provided in the PCB 250 may correspond to each of the plurality of holes 231h provided in the insulating plate 230 .

The positive electrode body 221 and the plurality of positive connectors A1 to A6 may constitute the positive electrode body 221 , and the positive electrode unit 220 includes the positive electrode body 221 without a separate member such as the PCB 250 . A first switch S1 connected in parallel with the first resistor R1 and a second switch S2 connected in parallel with the second resistor R2 may be provided.

8 is an enlarged view of an anode electrode part and an insulating plate according to an embodiment of the present invention.

Referring to FIG. 8 , the positive electrode part 220 includes a plurality of second resistors R2 connecting in series between the positive connectors A1 to A6 provided for each column in which the receiving spaces are arranged, and a A plurality of second switches S2 disposed in parallel with the second resistors R2 may be included to select whether to apply the second resistors R2 . The plurality of positive connectors A1 to A6 may be manufactured in the form of a wire capable of simple injection and bonding, and may be manufactured in the form of patterning or circuitry in order to simplify the weight and volume, but is not limited thereto.

The flow of current flowing through the second resistors R2 is determined depending on whether the second switches S2 are operated, and through this, the anodes 222 located in the same column of each accommodation space W. ), the same voltage or different voltages can be applied.

In an embodiment of the present disclosure, the positive electrode part 220 may include a plurality of positive connectors, and the positive connector may include a first resistor R1 .

Specifically, the first resistor R1 provided in the positive connector may include resistors connecting adjacent unit connectors (eg, C1 and C2) among the plurality of unit connectors C1 to C4 included in the positive connector. have.

The positive electrode part 220 includes a first resistor R1 connecting adjacent unit connectors in series among the unit connectors C1 to C4, and the first resistor to select whether to apply the first resistor R1. It may include a first switch (S1) disposed in parallel with (R1).

The plurality of unit positive connectors C1 to C4 may be manufactured in the form of simple injection and splicable wires, and may be manufactured in the form of patterning or circuitry in order to simplify the weight and volume, but is not limited thereto.

In the embodiment of the present disclosure, the flow of current flowing along the first resistors R1 is determined according to whether the first switches S1 are operated, and through this, the same column of each accommodation space W ), the same voltage or different voltages may be applied to the anodes 222 .

According to the embodiment configured as described above, even if the voltage variable structure is implemented, all of the positive connectors A1 to A6 and the positive electrodes 222 can be integrally manufactured or configured to be electrically coupled through a common power source, so it is simple can be produced

9 is a partially cut-away perspective view of an electric field applying device according to an embodiment of the present invention.

Referring to FIG. 9 , resistors R1 connecting the plurality of unit connectors C1 to C4 included in the positive connector in series are shown. In an embodiment of the present disclosure, a switch S1 connected in parallel to each of the resistors R1 may be included as shown in FIG. 9 .

In the embodiment of the present disclosure, since only the voltage provided to the anodes 222 can be varied for each accommodating space W by the operation of each switch S1, the electric field is applied to the accommodating spaces W arranged in the same column. may be formed uniformly or formed differently from each other. In addition, the electric field may be equally or differently formed for the accommodation spaces W arranged in different columns.

Therefore, it is possible to provide different voltages to each accommodation space W at once even without undergoing multiple tests. That is, it is easy to test the maximum number of samples in the shortest time, and it can be suitably used for high throughput analysis or screening.

10 is a diagram showing a first resistor and a second resistor according to an embodiment of the present invention;

11 is a diagram illustrating voltages applied to a plurality of accommodation spaces by the resistors shown in FIG. 10 .

Referring to FIG. 10 , the positive electrode part 220 is an example of a first resistor R1 provided in six positive connectors and a second resistor R2 connecting the six positive connectors in series to adjacent positive connectors. to be. At this time, each of the six positive connectors includes four unit connectors, and the four unit connectors C1 to C4 are connected to adjacent unit connectors by a resistor R. The first resistor R1 may include a resistor R connecting the four unit connectors.

When the resistor is arranged as shown in FIG. 10 , the voltage applied to each of the positive electrodes 222 corresponding to the unit connector through the positive electrode part 220 is as shown in FIG. 11 .

11 illustrates a voltage applied to each of the plurality of anodes 222 through the anode electrode unit 220 . Referring to FIG. 11 , by applying different voltages to the anode 222 corresponding to each unit connector, different electric fields can be formed in the accommodation spaces W arranged in the same column, and also the accommodations arranged in different columns. It is also possible to form an electric field differently for the spaces (W).

The present embodiment may be applied to a cover for applying an electric field capable of efficiently generating an electric field in a perforated plate and a device for applying a small capacity electric field including the same.

Claims (10)

a case coupled to an upper portion of a multi-well plate having a plurality of accommodating spaces in which the sample can be accommodated; and
and an electrode provided on the lower side of the case and applying a voltage in each accommodation space.
the electrode is
anode electrode part;
a cathode electrode part spaced apart from the anode electrode part; and
Including; an insulating plate insulating the positive electrode part and the negative electrode part;
The positive electrode part
a plurality of positive connectors provided for each column in which the receiving spaces are arranged;
a first resistor provided in the positive connector; and
a second resistor connecting adjacent positive connectors among the positive connectors in series;
Each of the plurality of positive connectors includes a plurality of unit connectors positioned above the accommodating space,
Each of the plurality of unit connectors is provided with a positive electrode accommodated in the receiving space,
The first resistor is
a resistor connecting adjacent unit connectors among the plurality of unit connectors;
Cover for electric field application. The method of claim 1
The positive electrode part
A cover for applying an electric field including an anode body provided with a cathode terminal and the plurality of unit connectors. 3. The method of claim 2
The positive electrode part
Further comprising a PCB provided with a first switch connected in parallel with the first resistor and a second switch connected in parallel with the second resistor,
Cover for electric field application. 4. The method of claim 3,
The PCB is provided with a plurality of through-holes through which the anode passes,
Cover for electric field application. 5. The method of claim 4
Each of the plurality of through-holes provided in the PCB has a corresponding
Cover for electric field application. 6. The method of claim 5,
The plurality of through-holes provided in the PCB substrate correspond to each of the plurality of holes provided in the insulating plate,
Cover for electric field application. According to claim 1,
The insulating plate is positioned below the both electrode parts and has a plurality of holes through which the anode passes,
The negative electrode part is positioned below the insulating plate and includes a plurality of negative electrodes positioned on inner peripheral surfaces of the accommodation spaces to be spaced apart from the positive electrodes, respectively,
Cover for electric field application. 2. The method of claim 1
The first resistors are arranged in a line while being spaced apart from each other in a first direction,
The second resistors are arranged in a line while being spaced apart from each other in a second direction crossing the first direction,
Cover for electric field application. According to claim 1,
The case and the electrode,
Air outlets are provided at positions corresponding to the accommodation spaces, respectively,
Cover for electric field application. A multi-well plate provided with a plurality of accommodation spaces in which the sample can be accommodated; and
The cover for applying an electric field according to any one of claims 1 to 11, which is provided detachably on the perforated plate and applies a voltage in each accommodation space when coupled on the perforated plate.
electric field application device.

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