KR20220122702A - Bacterial Delivery Vehicles for In Vivo Delivery of DNA Payloads - Google Patents

Abstract

The present disclosure relates generally to bacterial delivery vehicles and their use in the efficient delivery of a desired payload to target bacterial cells of a subject's microbiota. More particularly, the present disclosure relates to bacterial delivery vehicles having a preferred host range that can be used to efficiently deliver a desired payload in vivo to one or more target bacterial cells of a subject's microbiota.

Description

Bacterial Delivery Vehicles for In Vivo Delivery of DNA Payloads

The present disclosure relates generally to bacterial delivery vehicles and their use in the efficient delivery of a desired payload to a target bacterial cell population. More particularly, the present disclosure relates to bacterial delivery vehicles having a desirable host range that can be used to efficiently deliver a desired payload in vivo to one or more target bacterial cell populations of a microbiome.

DNA encapsulated in bacterial delivery particles can be used as a method for delivering genetic material to a target bacterial population. Several systems exist that allow the packaging of exogenous DNA into phage particles, such as bacteriophage lambda, in a laboratory setting. Such systems include, for example, systems for the direct production of packaged particles in bacterial cells and in vitro cell-free systems [1]-[3]. These systems take advantage of the fact that the addition of a cognate packaging site to an exogenous DNA vector (referred to as a phagemid, and more particularly a cosmid in the presence of a cos packaging site) allows for efficient packaging of these payloads into mature viral particles. This approach has been used in many different applications, for example for the generation of cosmid libraries or for the transduction of particular genes into bacteria [2], [4]. Most of these transduction assays are performed in laboratory conditions, cells are cultured in a controlled growth medium, such as LB, and transduction protocols are performed in buffer with known solute concentrations.

For in vivo applications, such as oral delivery of DNA encapsidated in particles, there is a need for a bacterial delivery vehicle that can be provided in a concentration high enough to reach all target cells, thus providing a payload that provides a sufficiently high titer. It is essential to optimize the manufacturing process as well as the in vivo activity. The DNA packaged in the particle must also be long enough and strong enough to bind its target cell for the injection process to occur.

Although it was previously confirmed that the presence of lateral tail fibers is not required for lambda-mediated in vitro transduction experiments in K-12 laboratory strains [5], lateral tail fibers are indeed essential for optimizing in vivo activity in treated subjects. It has been demonstrated in this specification. The present disclosure provides delivery vehicles for in vivo delivery to target host bacteria of interest, such as those in the gut of a subject to be treated.

The present disclosure relates to lambda-type bacterial delivery vehicles and their use in efficient in vivo delivery of desired payloads to target bacterial cells. A preferred payload comprises a nucleic acid molecule encoding a gene of interest.

Provided is a lambda-type bacterial delivery vehicle for use in in vivo delivery of a DNA payload of interest to a targeted bacterial cell population. In one embodiment, the bacterial delivery vehicle comprises one or more receptor binding protein(s) (RBPs). As used herein, a receptor binding protein or RBP recognizes a substrate located in the bacterial outer envelope, such as, but not limited to, the bacterial outer membrane, LPS, capsule, protein receptor, channel, structure such as flagella, glandular hair, secretory system, and optionally A polypeptide that binds and/or modifies or degrades. The substrate can be, without limitation, any carbohydrate or modified carbohydrate, any lipid or modified lipid, any protein or modified protein, any amino acid sequence, and any combination thereof.

In one embodiment, the bacterial delivery vehicle comprises one or more RBPs selected from the group consisting of a functional lambda-type flanking tail fiber protein (herein "STF protein"), a functional lambda-type gpJ protein and a functional lambda-type gpH protein. In another embodiment, the bacterial delivery vehicle may comprise two or more proteins selected from the group consisting of a functional lambda-type flanking tail fiber protein (herein "STF protein"), a functional lambda-type gpJ protein and a functional lambda-type gpH protein. have. In certain embodiments, the bacterial delivery vehicle comprises a functional lambda-type flanking tail fiber protein (herein "STF protein") and a functional lambda-type gpJ protein. In another embodiment, the bacterial delivery vehicle comprises a functional lambda flanking tail fiber protein (“STF protein” herein), a functional lambda gpJ protein and a functional lambda gpH protein. In another embodiment, the bacterial delivery vehicle comprises a functional lambda-type gpJ protein and a functional lambda-type gpH protein. In another aspect, the bacterial delivery vehicle comprises (i) a functional lambda-type flanking tail fiber protein. In addition to the functional STF protein, the bacterial delivery vehicle comprises (ii) a functional lambda-type gpJ protein; and optionally (iii) a functional lambda-type gpH protein.

In one embodiment, the STF protein, gpJ protein and/or gpH protein is a wild-type lambda STF, gpJ and/or gpH protein. Alternatively, the STF protein, gpJ protein and/or gpH protein is a recombinant protein, preferably a non-naturally occurring recombinant protein. In particular, recombinant STF protein, gpJ protein and/or gpH protein can be engineered to target delivery of a DNA payload of interest to a targeted bacterial cell. In a non-limiting example, the recombinant STF protein may be engineered to advantageously retain enzymatic activity such as depolymerase activity and the target bacterial cell may be an encapsulated bacterial cell. Such depolymerase activity has been shown to increase delivery efficiency and includes an activity associated with endoliadase such as, for example, an activity associated with K1F endoliadase or a lyase, such as an activity associated with, for example, K5 lyase. do.

Recombinant STF proteins include, for example, engineered chimeric STF proteins and in some instances, the present disclosure provides chaperone (or auxiliary) proteins with which they are associated. This chaperone protein assists in the folding of the chimeric STF protein. The recombinant engineered chimeric STF protein may comprise a fusion between a portion of an STF protein derived from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and a portion of an STF protein derived from a corresponding STF protein derived from a different bacteriophage. can Such a chimeric STF protein may comprise a fusion between the N-terminal domain of an STF from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and the C-terminal domain of a different STF. In one embodiment, the chimeric STF protein comprises the amino acid sequence of SEQ ID NO: 14, the amino acid sequence of SEQ ID NO: 16, the amino acid sequence of SEQ ID NO: 17, the amino acid sequence of SEQ ID NO: 19, SEQ ID NO: 21 comprises or consists of the amino acid sequence of, the amino acid sequence of SEQ ID NO: 44, or the amino acid sequence of SEQ ID NO: 50. In certain embodiments, the chimeric STF protein is STF-V10 (SEQ ID NO: 44). Other examples of chimeric STF proteins include STF-V10f (SEQ ID NO: 45), STF-V10a (SEQ ID NO: 46) and STF-V10h (SEQ ID NO: 47). The present disclosure also provides synthetic bacterial delivery vehicles characterized by the presence of engineered branched receptor binding multi-subunit protein complexes (“branched-RBPs”). An engineered branched-RBP comprises two or more associated receptor binding proteins, derived from a bacteriophage, that associate with each other based on the presence of an interacting domain (ID). In certain embodiments, the engineered branched-RBP comprises two or more associated STFs, derived from a bacteriophage, that associate with each other based on the presence of an ID. The association of one subunit with another may be non-covalent or covalent. Each polypeptide subunit contains an ID that serves as an "anchor" for the association of one subunit RBP with another. In certain embodiments a branched-RBP may comprise multiple RBP subunits, including, for example, 2, 3, 4, etc. subunits. Individual RBP subunits can impart different biological functions to the entire engineered branched-RBP. These functions include, without limitation, host recognition and enzymatic activity. Such enzymatic activity includes depolymerase activity.

Accordingly, a bacterial delivery vehicle is provided that enables the delivery of a nucleic acid payload encoding a protein or nucleic acid of interest to a desired target bacterial host cell, said bacterial delivery vehicle comprising a chimeric STF and/or minute as disclosed herein. It is characterized as having a topography-RBP.

Bacterial delivery vehicles are also provided that contain recombinant gpJ protein. Such gpJ proteins include recombinant gpJ proteins, including chimeric proteins, which allow recognition of bacterial cell receptors other than the LamB OMP receptor, a natural lambda phage receptor on the bacterial cell surface (14). The recombinant engineered chimeric gpJ protein may comprise a fusion between a portion of a gpJ protein derived from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and a portion of a gpJ protein derived from a corresponding gpJ protein derived from a different bacteriophage. have. Such a chimeric gpJ protein may comprise a fusion between the N-terminal domain of a gpJ protein from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and the C-terminal domain of a different gpJ protein. In one embodiment, the gpJ protein comprises or consists of the amino acid sequence of SEQ ID NO: 10, 11, 12, 13 or 49.

Bacterial delivery vehicles are also provided that contain recombinant gpH protein. Such gpH proteins include recombinant gpH proteins that allow or allow improved entry of bacterial vectors into cells with alterations or defects in the permease complex. The recombinant engineered chimeric gpH protein may comprise a fusion between a portion of a gpH protein derived from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and a portion of a gpH protein derived from a corresponding gpH protein derived from a different bacteriophage. have. Such chimeric gpH proteins may comprise a fusion between the N-terminal domain of a gpH protein from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and the C-terminal domain of a different gpH protein. In one embodiment, the gpH protein comprises or consists of the amino acid sequence of SEQ ID NO: 23 or 24.

In certain aspects, the bacterial delivery vehicles provided herein are engineered to increase the efficiency of delivery of a DNA payload to bacterial cells in which the recombinant STF protein, gpJ protein and/or gpH protein is targeted as the vehicle. These bacterial cells are Yersinia spp., Escherichia spp., Klebsiella spp., Acinetobacter spp., Pseudomonas spp., Helicobacter spp., Vibrio spp, Salmonella spp., Streptococcus spp., Staphylococcus spp., Bacteroides spp., Clostridium spp., Shigella spp., Enterococcus spp., Enterobacter spp. , Listeria spp, and mixtures thereof, preferably E. E. coli and other bacterial species of interest, such as, for example, Klebsiella, Citrobacter , Agrobacterium , Enterobacter or Pseudomonas, more preferably selected from the group consisting of this. it's coli

The bacterial delivery vehicles disclosed herein provide a means for delivery, including in vivo delivery of a DNA payload of interest to a targeted host bacterium. In a non-limiting aspect, the DNA payload comprises a Cas nuclease gene, a Cas9 nuclease gene, a guide RNA, a CRISPR locus, a toxin gene, an enzyme such as a nuclease or kinase, TALEN, ZFN, meganuclease, recombi. Genes encoding nases, bacterial receptors, membrane proteins, structural proteins, secreted proteins, genes encoding resistance to antibiotics or over-the-counter drugs, genes encoding toxic proteins or virulence factors, and virulence proteins or virulence factors encoding genes a nucleic acid of interest selected from the group consisting of a gene, or any combination thereof. In certain embodiments, the nucleic acid of interest encodes a therapeutic protein. Still further, the nucleic acid of interest encodes an antisense nucleic acid molecule.

In one aspect, the bacterial delivery vehicle enables delivery of a nucleic acid payload encoding a nuclease that targets cleavage of the host bacterial cell genome or host bacterial cell plasmid. In some embodiments, the cleavage occurs in an antibiotic resistance gene. In other embodiments, nuclease mediated cleavage of the host bacterial cell genome is designed to stimulate a homologous recombination event for insertion of a nucleic acid of interest into the genome of the bacterial cell.

The present disclosure also provides a pharmaceutical or veterinary composition comprising one or more bacterial delivery vehicles disclosed herein and a pharmaceutically acceptable carrier. Also provided is a method for treating a disease or disorder caused by a bacterium, preferably a bacterial infection, the method being provided to a subject having a disease or disorder caused by a bacterium, preferably a bacterial infection, in need thereof administering a pharmaceutical or veterinary composition. The present disclosure also relates to a pharmaceutical or veterinary composition or bacterial delivery vehicle as disclosed herein for use in the treatment of a disease or disorder caused by bacteria, preferably a bacterial infection. It also relates to the use of a pharmaceutical or veterinary composition or bacterial delivery vehicle as disclosed herein for the manufacture of a medicament for treating a disease or disorder caused by bacteria, preferably a bacterial infection. The disease or disorder caused by bacteria is preferably selected from bacterial infections, metabolic disorders and pathologies involving bacteria of the human microbiome. More preferably, the disease or disorder caused by bacteria is a bacterial infection. A method for reducing the amount of virulent and/or antibiotic resistant bacteria in a bacterial population is provided, the method comprising contacting the bacterial population with a bacterial delivery vehicle disclosed herein. The method may be an in vivo or in vitro method. The present disclosure also relates to a pharmaceutical or veterinary composition or bacterial delivery vehicle as disclosed herein for use in reducing the amount of virulent and/or antibiotic resistant bacteria in a bacterial population, particularly in a subject having a bacterial infection. It also relates to the use of a pharmaceutical or veterinary composition as disclosed herein or a bacterial delivery vehicle for the manufacture of a medicament for reducing the amount of virulent and/or antibiotic resistant bacteria in a bacterial population, particularly in a subject having a bacterial infection.

In another aspect, the methods and compositions described herein provide for long-term stable expression of a gene of interest in a microbiome of a host. In this example, the delivery vehicle comprises a nucleic acid molecule encoding a gene of interest, the nucleic acid being integrated into the bacterial chromosome, or alternatively engineered to replicate stably within the targeted microbiome of the host. Upon delivery to the bacterium of interest, ie, the microbiome, typically the gene of interest will be expressed. The methods and compositions described herein encompass the in situ bacterial production of any compound of interest, including therapeutic compounds such as prophylactic and therapeutic vaccines for mammals. The compound of interest may be produced inside the targeted bacterium, secreted from the targeted bacterium, or expressed on the surface of the targeted bacterium. In a more specific embodiment, the antigen is expressed on the surface of a targeted bacterium for prophylactic and/or therapeutic vaccination.

In order to better understand the subject matter disclosed herein and to illustrate how it may be practiced in practice, embodiments will now be described by way of non-limiting example with reference to the accompanying drawings. With particular reference to the drawings, it is emphasized that the details shown are for the purpose of and examples of illustrative descriptions of implementations of the present disclosure.
Figure 1. Presence of transducants after oral gavage of lambda-PaPa packaged psgRNAcos cosmid (DNA payload of SEQ ID NO: 1). Black dots, total number of MG-GFP cells. White dots, MG-GFP cells acquiring kanamycin resistance.
Figure 2. In vivo adaptation of lambda PaPa phage to MG-GFP in the gut of mice (n=3). Each line corresponds to an experiment performed on one mouse. X axis, number of days.
Figure 3. Presence of transducants after oral gavage of Ur-lambda packaged pJ23104-GFP cosmid (3 kbp) (DNA payload of SEQ ID NO: 2). Black dots, total number of MG1655-Str cells. White dots, MG-GFP cells that acquired chloramphenicol resistance.
Figure 4. Presence of transducants after oral gavage of Ur-lambda packaged pJF1 cosmid (7 kb) (DNA payload of SEQ ID NO: 3). Black dots, total number of MG-GFP cells. White dots, MG-GFP cells that acquired chloramphenicol resistance.
5. Titration of packaged lambda phagemids with different payload sizes.
6A - B. Delivery efficiency after oral gavage of Ur-lambda packaged GG6K and GG8K cosmids (SEQ ID NO: 6 and SEQ ID NO: 7, respectively) with or without stf. Figure 6A. Packaged phagemids with STF present. Figure 6B Packaged phagemids in the absence of STF.
7 . Alignment of gpJ variants to lambda gpJ. Two insertion points based on protein identity, indicated by boxes 1 and 2, were chosen to generate chimeras with lambda gpJ.
8A - D . Apparent titers of different gpJ chimeras. Figure 8A . 591 chimera, inserted into lambda gpJ using the second insertion point (box #2 in Figure 7). Lambda WT refers to the native gpJ variant that recognizes LamB. Each lane represents a 10-fold dilution of the packaged phagemid produced, most concentrated on the right and most diluted on the left. Figure 8B . Apparent titers of gpJ variants lambda WT, Z2145 and 1A2 (SEQ ID NO: 10, SEQ ID NO: 12 and SEQ ID NO: 13, respectively) in three strains: MG-GFP (black bars), MG-delta-LamB (white bars) and H10-waaJ (O157 strain lacking the O157 antigen, gray bars). Figure 8C . H10 wt strain (containing group 4 capsules) using lambda packaged phagemid with gpJ Z2145 variant (SEQ ID NO: 12) or Z2145 (SEQ ID NO: 12) with WW11.2 stf variant (SEQ ID NO: 16) ) as measured by flow cytometry (% GFP+ cells). Figure 8D . Lambda packaged phagemids comprising gpJ variant A8 (SEQ ID NO: 49) or 1A2 (SEQ ID NO: 13) and chimeric lambda-P2 STF (SEQ ID NO: 50) in transduced MG1655 or MG1656-OmpCO157 Transduction efficiency (% GFP+ cells) as measured by flow cytometry.
Figure 9 AB. Analysis of lambda gpH and generation of engineered variants. Figure 9A . this. Alignment between lambda gpH and gpH proteins from different lambda-type prophages found in E. coli. Figure 9B . Titration of lambda WT gpH variants (left panel - SEQ ID NO: 23) and engineered gpH-IAI (right panel - SEQ ID NO: 24) in MG1655, manZ and manY mutants. Each lane represents a 10-fold dilution of the packaged phagemid produced, most concentrated on the right and most diluted on the left.
Figure 10 . Delivery efficiency of engineered lambda-packaged phagemids in different proteobacteria. Enterobacter Point titration of different gpJ and STF combinations against Enterobacter cloacae strains. 10 μl of packaged phagemid was mixed with 90 μl of bacteria with an OD600-0.7, incubated at 37° C. for 30 minutes, and 10 μl of the reaction was plated on LB agar supplemented with 25 μg/mL chloramphenicol.
Figure 11. Stability of 1A2-STF118 or 1A2-STF29 packaged phagemids in PBS. gray bars, PBS alone; White bars, PBS with pancreatin, pH 6.8. left group of bars, activity in MG1656-OmpCO157; Right group of bars, strain LMR_503. Y axis shows particle titer per μl.
12. Overlay of sedimentation coefficient distribution data of three Eligobiotics® (EB) batches analyzed with svAUC in Example 3. The range of integration for EBs packaged with 3 or 4 copy number of payloads is shown in dashed lines.
13 . Relative abundance of Eligobiotics® containing 3 or 4 copies of their payload. The absorbance signals at 260 and 280 nm for each population defined as svAUC were integrated and used to calculate their relative abundance in each batch of Eligobiotics®.

The present disclosure relates to bacterial delivery vehicles having a desirable host range that can be used to efficiently deliver a desired payload in vivo to one or more target bacterial cells of a subject's microbiota.

Disclosed herein are methods and compositions for in vivo delivery of a desired payload to a microbiome of a subject. Such delivery vehicles are engineered to contain, as part of their payload, nucleic acids encoding RNA molecules or proteins that may be useful in the treatment of disorders and diseases in a subject. Such nucleic acids can encode, by way of non-limiting example, generally any molecule, compound, and protein.

Bacterial delivery vehicles provided herein allow for delivery of a nucleic acid payload encoding a protein or nucleic acid of interest to a desired target bacterial host cell. As used herein, the term “delivery vehicle” means any means that permits delivery of a payload to a bacterium. Without limitation, bacteriophage scaffolds, viral scaffolds, chemical based delivery vehicles (eg, cyclodextrins, calcium phosphate, cationic polymers, cationic liposomes), protein-based or peptide-based delivery vehicles, lipid-based delivery vehicles , nanoparticle-based delivery vehicles, non-chemical-based delivery vehicles (eg, transformation, electroporation, sonoporation, optical transfection), particle-based delivery vehicles (eg, gene gun, magnetofection) There are several types of delivery vehicles encompassed by this disclosure, including , impalofection, particle bombardment, cell-penetrating peptides) or donor bacteria (conjugated). Any combination of delivery vehicles is also encompassed by the present disclosure. Delivery vehicle may refer to a bacteriophage derived scaffold and may be obtained from a natural, evolved or engineered capsid.

In one aspect, a bacterial delivery vehicle having a desired target host range is delivered for use in in vivo delivery of a payload to a microbiome of a subject. The bacterial delivery vehicle may comprise one or more proteins selected from the group consisting of a functional lambda-type flanking tail fiber protein ("STF protein" herein), a functional lambda-type gpJ protein and a functional lambda-type gpH protein. In another embodiment, the bacterial delivery vehicle may comprise two or more proteins selected from the group consisting of a functional lambda-type lateral tail fiber protein, a functional lambda-type gpJ protein and a functional lambda-type gpH protein. In certain embodiments, the bacterial delivery vehicle comprises a functional lambda-type STF protein and a functional lambda-type gpJ protein. In another embodiment, the bacterial delivery vehicle may comprise a functional lambda-type STF protein, a functional lambda-type gpJ protein and a functional lambda-type gpH protein. In another aspect, the bacterial delivery vehicle may comprise a functional lambda-type gpJ protein and a functional lambda-type gpH protein. In another aspect, the bacterial delivery vehicle may comprise (i) a functional lambda-type STF protein. In addition to the functional STF protein, the bacterial delivery vehicle includes (ii) a functional lambda-type gpJ protein; and optionally (iii) a functional lambda-type gpH protein.

In one embodiment, the functional STF protein, functional gpJ protein and/or functional gpH protein are wild-type lambda STF, gpJ and/or gpH protein, respectively. Alternatively, the functional STF protein, functional gpJ protein and/or functional gpH protein is a recombinant protein.

As used herein, the term “recombinant protein” refers to a non-naturally occurring protein obtained through recombinant technology, particularly an engineered protein. Such recombinant proteins include, for example, engineered chimeric proteins.

As used herein, a functional protein generally refers to a protein having biological activity; More particularly, as used herein, a functional wild-type, recombinant protein, variant, fusion or fragment refers to a wild-type, recombinant protein, variant, fusion or fragment that contributes to the efficient delivery of a DNA payload to a target strain. Efficiency thresholds depend on a number of factors such as protein type, target strain type and environment type. For example, STF and gpJ proteins allow recognition, binding (and in some cases also degradation) of extracellular epitopes such as LPS, capsular and outer membrane proteins; The gpH protein allows efficient injection and thus successful passage of the DNA payload through the periplasm.

In the context of the present invention, proteins such as STF, gpJ and gpH proteins are titrated to a packaged phagemid containing said protein on bacterial cells known to display a receptor recognized by said protein and not recognized by said protein. Functionality can be determined by comparison to titers obtained with the same packaged phagemids on bacterial cells known to display receptors that do not.

This recombinant chimeric STF protein comprises a portion of an STF protein derived from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and an STF protein derived from a STF protein from a different bacteriophage, in particular a different lambda-type bacteriophage or a non-lambda-type bacteriophage. (also referred to herein as “chimeric receptor binding protein” or “chimeric RBP”). Such a chimeric STF protein may comprise a fusion between the N-terminal domain of an STF from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and the C-terminal domain of a different STF. As used herein, a receptor binding protein or RBP recognizes a substrate located on the bacterial outer envelope, such as, but not limited to, the bacterial outer membrane, LPS, capsule, protein receptor, channel, structure such as flagella, glandular hair, secretory system, It may optionally be an STF-derived polypeptide that binds and/or modifies or degrades. The substrate can be, without limitation, any carbohydrate or modified carbohydrate, any lipid or modified lipid, any protein or modified protein, any amino acid sequence, and any combination thereof.

As used herein, lambda-type bacteriophages include a group of related viruses that infect bacteria. Viruses are said to be of the lambda form because one of the first members described is lambda (λ). Lambda-type bacteriophages are members of the caudovirus order (also called tail-type bacteriophages) and are similar, including, for example, prophages inducible by UV irradiation, the ability to recombine when crossed, retention of the same pair of cohesive ends, and bacteriophages having a lifestyle. Although members of this order may have genomes that are variable at the nucleotide level, they typically possess sufficient regions of nucleotide sequence identity to guide recombination among themselves, providing fully functional phages with all essential genes (cf. : see, for example, Casjens and Hendrix (2015) Virology 479-480:310-330). For the purposes of this disclosure, lambda-type bacteriophages for use as delivery vehicles as well as lambda-type STF, gpH and gpJ proteins for use will be generally understood by those skilled in the art.

Lambda-type phage can be defined as belonging to the lambda supercluster based on genomic analysis [6]. Within these superclusters, several clusters can be distinguished, each with a prototype phage. Phage-like clusters and their members (in parentheses) are: lambda-like (lambda (λ), HK630, HK629), phi80-like (phi80, HK225, mEp237), N15-like (N15, PY54, phiKO2) ), HK97-like (HK97, HK022, HK75, HK106, HK140, HK446, HK542, HK544, HK633, mEpX1, mEpX2, mEp234, mEp235, mEp390, ENT393UB), ES18, Oslo, SPN3UB (ES18, Oslo, SPN3UB) 2-like (gifsy-2, gifsy-1, Fels-1, mEp043, mEp213, CP-1639, CTD-Iø, mEp640, FSL_SP-016), BP-4795-like (BP-4795, 2851, stx2-1717) , YYZ-2008), SfV-like (SfV, SfII, SfIV, SfI, øP27, ST64B), P22-like (P22, L, SPN9CC, ST64T, ST104, ST160, epsilon34, g341, SE1, Emek, φ20, IME10, Sf6, HK620, CUS-3, SPC-P1), APSE-1-like (APSE-1, APSE-2), 933W-like (933W, stx1ø, stx2ø-I, stx2ø-II, stx2-86, min27, ø24B, P13374, TL-2011c, VT2-Sakai, VT2ø_272), HK639-like (HK639), øES15-like (øES15), HS2-like (HS2), ENT47970-like (ENT47670), ZF40-like (ZF40) ), øEt88-like (øEt88).

In the present disclosure, the lambda-type STF protein comprises, for example, an amino acid sequence that has at least 75% identity to amino acid or less corresponding to amino acid 130 of lambda STF (Uniprot P03764 SEQ ID NO: 14), in particular to amino acid 130 or less of said lambda STF. a protein comprising or consisting of; A lambda-type gpJ protein is a protein comprising or consisting of an amino acid sequence that has at least 35% identity to amino acid 606 or less corresponding to amino acid 606 of lambda gpJ (Uniprot P03749 SEQ ID NO: 10), in particular to amino acid 606 or less of said lambda gpJ. including; A lambda-type gpH protein includes a protein comprising or consisting of an amino acid sequence having at least 40% identity over the entire length of lambda gpH (Uniprot P03736 SEQ ID NO: 23), wherein the amino acid stretch between positions 189 to 391 indicates the identity. Consider that you may have little or no. The lambda-type bacterial delivery vehicle includes a bacterial delivery vehicle comprising a functional lambda-type stf protein and/or a functional lambda-type gpJ protein and/or a functional lambda-type gpH protein, each of which may have an altered host range compared to a wild-type lambda phage. include

In one aspect, the STF protein comprises at least 80, 85, 90, 95, 96, 97, the wild-type lambda stf protein amino acid sequence of SEQ ID NO: 14, or any recombinant STF protein, fusion, variant or fragment disclosed herein. protein comprising or consisting of an amino acid sequence having 98, or 99% sequence identity. In one aspect, the gpJ protein comprises at least 80, 85, 90, 95, 96, 97, 98 with the wild-type gpJ protein amino acid sequence of SEQ ID NO: 10, or any recombinant gpJ protein, fusion, variant or fragment disclosed herein. , or a protein comprising or consisting of an amino acid sequence having 99% sequence identity. In one aspect, the gpH protein comprises at least 80, 85, 90, 95, 96, 97, 98 with the wild-type gpH protein amino acid sequence of SEQ ID NO: 23, or any recombinant gpH protein, fusion, variant or fragment disclosed herein. , or a protein comprising or consisting of an amino acid sequence having 99% sequence identity. In another aspect, provided herein are nucleic acids encoding such wild-type, or recombinant, STF, gpH and gpJ proteins.

As used herein, % homology between two sequences is equivalent to % identity between two sequences. % identity is calculated for a polymer (eg, polynucleotide or polypeptide) to which the sequence is aligned. The % identity between two sequences is a function of the number of identical positions shared by the sequences, taking into account the number of gaps, and the length of each gap, that need to be introduced for optimal alignment of the two sequences (i.e., % Homology = same position #/total position # x 100). Comparison of sequences and determination of percent identity between two sequences can be performed using a mathematical algorithm, as described in the non-limiting examples below.

The % identity between the two amino acid sequences was introduced into the ALIGN program (version 2.0), using the PAM120 weighted residue table, a gap length penalty of 12 and a gap penalty of 4, [E. Meyers and W. Miller (Comput. Appl. Biosci., 4: 11-17 (1988))]. In addition, the % identity between the two amino acid sequences is a BLOSUM62 matrix, a BLOSUM30 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and 1, 2, 3, 4, 5, or 6 to be determined using the algorithm [Nedleman and Wunsch (J. Mol. Biol. 48:444-453 (1970))] introduced in the GAP program in the GCG software package (available at www.gcg.com), using the weights of can In a specific implementation, the BLOSUM30 matrix uses a gap opening penalty of 12 and a gap extension penalty of 4 .

A variety of different lambda-type bacterial delivery vehicles serve as a means for delivery of a payload to a target bacterial cell population. Such bacterial delivery vehicles include those comprising one or more wild-type lambda-type STF, gpH and gpJ proteins. Alternatively, the delivery vehicle may comprise one or more wild-type STF, gpH, or gpJ proteins in combination with one or more recombinant STF, gpH, or gpJ proteins, including chimeric proteins, fusions, variants or fragments as disclosed herein. can delivery vehicles in which the three STF, gpH and gpJ proteins are wild-type and delivery vehicles in which the three STF, gpH and gpJ proteins are recombinant, fusion, variant or fragment.

The present disclosure provides a delivery vehicle comprising a chimeric receptor binding protein (RBP), wherein the chimeric RBP is an N-terminal domain of an RBP from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and a C of a different bacteriophage RBP. -Contains fusions between the terminal domains. Such bacteriophage RBPs from which chimeric RBPs are derived include, for example, "L-shaped fibers", "lateral tail fibers (STFs)", "long tail fibers" or "tail spikes". This bacteriophage RBP from which the chimeric RBP is derived may be a wild-type RBP or an RBP variant, preferably a wild-type RBP. Such chimeric RBPs include those with altered host range and/or biological activity such as, for example, depolymerase activity. In one embodiment, a chimeric RBP has a host range that is assigned to a particular bacterial cell of the host or subject microbiome. In one specific embodiment, the different RBPs of the chimeric receptor binding protein (RBP) are derived from any bacteriophage or any bacteriocin.

Such a chimeric RBP may comprise a fusion between the N-terminal domain of an RBP from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and the C-terminal domain of a different RBP. The N-terminal domain is typically fused to the N-terminal end of the C-terminal domain.

RBP from a bacteriophage, in particular the "N-terminal domain" of the STF protein, as used herein, refers to the RBP starting at the N-terminal end of the RBP and ending at positions 80-150, 320-460 or 495-560 of the RBP. amino acid region, wherein the position is relative to the lambda bacteriophage STF sequence (SEQ ID NO: 14). The "C-terminal domain" of an RBP, in particular an STF protein, from a bacteriophage, as used herein, refers to an RBP starting at positions 25-150, 320-460 or 495-560 of the RBP and ending at the C-terminus of the RBP. amino acid region, wherein the position is relative to the lambda bacteriophage STF sequence (SEQ ID NO: 14).

In one embodiment, the bacterial delivery vehicle contains a chimeric RBP comprising a fusion between the N-terminal domain of an RBP derived from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and the C-terminal domain of a different RBP, wherein said N-terminal domain of a chimeric RBP differs within one of the amino acid regions selected from positions 80-150, 320-460, or 495-560 of the N-terminal domain with respect to the lambda bacteriophage STF sequence (SEQ ID NO: 14). fused to the C-terminal domain of In one aspect, the RBP from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and a different RBP are at positions 80-150, 320-460, and 495 of the RBP with respect to the lambda bacteriophage STF sequence (SEQ ID NO: 14). contain homology in at least one of the three amino acid regions ranging from -560. In certain aspects, the homology is approximately 35% identity to at least 45 amino acids within one or more of the three amino acid regions ranging from positions 80-150, 320-460, and 495-560 of the RBP to the lambda bacteriophage STF sequence, 30 amino acids approximately 50% identity to at least 18 amino acids, or approximately 90% identity to at least 18 amino acids. In one particular embodiment, the different RBP domains of the chimeric RBP are derived from a bacteriophage or a bacteriocin. In one aspect, the chimeric RBP differs within one of the amino acid regions selected from positions 80-150, 320-460, or 495-560 of the N-terminal RBP domain with respect to the lambda bacteriophage STF sequence (SEQ ID NO: 14). and the N-terminal domain of RBP fused to the C-terminal domain of In other non-limiting embodiments, the chimeric RBP comprises amino acids SAGDAS (SEQ ID NO: 37), ADAKKS (SEQ ID NO: 38), MDETNR (SEQ ID NO: 39), SASAAA (SEQ ID NO: 40), and GAGENS (SEQ ID NO: 41) of different RBPs fused within a site of an N-terminal RBP domain having at least 80%, 85%, 90%, 95%, 99% or 100% identity to a site selected from the group consisting of (SEQ ID NO: 41). It contains a C-terminal domain and an N-terminal domain of RBP.

In certain embodiments, the chimeric STF protein comprises a fusion between the N-terminal domain of a lambda bacteriophage STF protein and the C-terminal domain of an STF protein from another bacteriophage, wherein said N-terminal domain is in particular a lambda bacteriophage STF protein sequence (SEQ ID NO: ID NO: 14) to the C-terminal domain in the N-terminal amino acid region 495-560. In this embodiment, the chimeric STF variant may be STF-V10 comprising or consisting of the amino acid sequence SEQ ID NO: 44 and typically encoded by the nucleotide sequence SEQ ID NO: 51. Alternatively, in this embodiment, the chimeric STF variant may be WW11.2 comprising or consisting of the amino acid sequence SEQ ID NO: 16 and typically encoded by the nucleotide sequence SEQ ID NO: 30. Still alternatively, in this embodiment, the chimeric STF variant may be STF75 comprising or consisting of the amino acid sequence SEQ ID NO: 17 and typically encoded by the nucleotide sequence SEQ ID NO: 31. In this embodiment, the STF75 can be produced in particular with its associated chaperone protein, which typically comprises or consists of the amino acid sequence SEQ ID NO: 18 and is typically encoded by the nucleic acid sequence SEQ ID NO: 34. . Still alternatively, in this embodiment, the chimeric STF variant may be STF23 comprising or consisting of the amino acid sequence SEQ ID NO: 21 and typically encoded by the nucleotide sequence SEQ ID NO: 35. In this embodiment, the STF23 may be produced in particular with its associated chaperone protein, which typically comprises or consists of the amino acid sequence SEQ ID NO: 22 and is typically encoded by the nucleic acid sequence SEQ ID NO: 36. have. In another embodiment, the chimeric STF protein comprises a fusion between the N-terminal domain of a lambda bacteriophage STF protein and the C-terminal domain of an STF protein from another bacteriophage, wherein said N-terminal domain is in particular a lambda bacteriophage STF protein sequence (SEQ ID NO: ID NO: 14) to the C-terminal domain in the amino acid region 320-460 of the N-terminal domain. In this embodiment, the chimeric STF variant may be STF-EB6 comprising or consisting of the amino acid sequence SEQ ID NO: 19 and typically encoded by the nucleotide sequence SEQ ID NO: 33. In this embodiment, said STF-EB6 is produced in particular with its associated chaperone protein, typically comprising or consisting of the amino acid sequence SEQ ID NO: 20, and typically encoded by the nucleic acid sequence SEQ ID NO: 32 can be Alternatively, in this embodiment, the chimeric STF variant may be an STF lambda-P2 comprising or consisting of the amino acid sequence SEQ ID NO: 50 and typically encoded by the nucleotide sequence SEQ ID NO: 56. In this embodiment, the STF lambda-P2 in particular comprises or consists of the amino acid sequence SEQ ID NO: 57, and typically can be produced together with its associated chaperone protein encoded by the nucleic acid sequence SEQ ID NO: 58. have. Alternatively, the chimeric STF variant may be STF-V10f comprising or consisting of the amino acid sequence SEQ ID NO: 45 and typically encoded by the nucleic acid sequence SEQ ID NO: 52. Alternatively, the chimeric STF variant may be STF-V10a comprising or consisting of the amino acid sequence SEQ ID NO: 46 and typically encoded by the nucleic acid sequence SEQ ID NO: 53. Alternatively, the chimeric STF variant may be STF-V10h comprising or consisting of the amino acid sequence SEQ ID NO: 48 and typically encoded by the nucleic acid sequence SEQ ID NO: 54.

Recombinant RBP proteins as disclosed herein may require their associated chaperones (also called adjuvants) for proper folding. This chaperone protein assists in the folding of the chimeric RBP protein. For example, a lambda STF protein comprising or consisting of the amino acid sequence SEQ ID NO: 14 typically requires its associated protein comprising or consisting of the amino acid sequence SEQ ID NO: 15 for proper folding. The need for an associated chaperone protein for proper folding of the chimeric RBP protein typically depends on the RBP from which the C-terminal region of the chimeric RBP is derived. For example, the chimeric STF protein STF75 comprising or consisting of the amino acid sequence SEQ ID NO: 17 typically comprises or consists of the amino acid sequence SEQ ID NO: 18, typically the nucleic acid sequence SEQ ID It requires its associated chaperone protein, encoded by NO: 34. For example, the chimeric STF protein STF-EB6 comprising or consisting of the amino acid sequence SEQ ID NO: 19, for proper folding, typically comprises or consists of the amino acid sequence SEQ ID NO: 20, and typically consists of a nucleic acid sequence It requires its associated chaperone protein encoded by SEQ ID NO: 32. For example, the chimeric STF protein STF23 comprising or consisting of the amino acid sequence SEQ ID NO: 21 typically comprises or consists of the amino acid sequence SEQ ID NO: 22, typically the nucleic acid sequence SEQ ID It requires its associated chaperone protein, encoded by NO: 36. For example, the chimeric STF protein STF lambda-P2 comprising or consisting of the amino acid sequence SEQ ID NO: 50 typically comprises or consists of the amino acid sequence SEQ ID NO: 57, typically a nucleic acid, for proper folding. It requires its associated chaperone protein encoded by the sequence SEQ ID NO: 58. The chaperone protein may remain attached to the chimeric STF protein after folding. Thus, in certain embodiments, the bacterial delivery vehicle disclosed herein may further comprise a chaperone protein associated with the chimeric STF protein it comprises. Alternatively, the chaperone protein may not remain attached to the chimeric STF protein after folding, eg may be proteolytically, in particular self-proteolytic. Accordingly, in certain embodiments, the bacterial delivery vehicles disclosed herein do not comprise a chaperone protein associated with the chimeric STF protein that the vehicle comprises.

The present disclosure also provides synthetic bacterial delivery vehicles characterized by the presence of engineered branched receptor binding multi-subunit protein complexes (“branched-RBPs”). Such delivery vehicles can be used to deliver the payload of interest to bacterial cells of the microbiome. An engineered branched-RBP comprises two or more associated receptor binding proteins, derived from a bacteriophage, that are associated with each other based on the presence of an interacting domain (ID). The association of one subunit with another may be non-covalent or covalent. Each polypeptide subunit contains an ID that serves as an "anchor" for the association of one subunit RBP with another. In certain embodiments, a branched-RBP may comprise multiple RBP subunits, including, for example, 2, 3, 4, etc. subunits.

Individual RBP subunits can impart different biological functions to the entire engineered branched-RBP. These functions include, without limitation, host recognition and enzymatic activity. Such enzymatic activity includes depolymerase activity. Two or more associated receptor binding proteins of branched-RBP include, without limitation, a fusion between the N-terminal domain of an RBP derived from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and a C-terminal domain of a different RBP. A chimeric receptor binding protein (RBP) described herein, wherein the chimeric RBP further comprises an ID domain.

Accordingly, a bacterial delivery vehicle is provided that enables the delivery of a nucleic acid payload, encoding a protein or nucleic acid of interest, to a desired target bacterial host cell, wherein the bacterial delivery vehicle is a chimeric-RBP or antigen as disclosed herein. Characterized as having a topography-RBP (for chimeric and branched RBPs, see US Provisional Application No. 62/802,777, US Provisional Application No. 16/696,769, and US Provisional Application No. 16/726,033, each of which is in its entirety incorporated herein by this reference).

Bacterial delivery vehicles comprising the recombinant gpJ protein are also provided. Such gpJ proteins include recombinant gpJ proteins, including chimeric proteins, which allow recognition of bacterial cell receptors in addition to the LamB OMP receptor. The receptor-recognition activity of gpJ is present in the C-terminal portion of the protein, and fragments as small as 249 aa are known to confer the ability to bind to the LamB receptor [5]. In certain embodiments, such a chimeric gpJ protein may comprise a fusion between the N-terminal domain of a gpJ protein from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and the C-terminal domain of a different gpJ protein. The N-terminal domain is typically fused to the N-terminal end of the C-terminal domain.

The "N-terminal domain" of the gpJ protein from a bacteriophage, as used herein, refers to an amino acid region of the gpJ protein that starts at the N-terminal end of the gpJ protein and ends at positions 810-825 or 950-970 of the gpJ protein. and the position is for the lambda bacteriophage gpJ protein sequence (SEQ ID NO: 10). The "C-terminal domain" of the gpJ protein from a bacteriophage as used herein refers to an amino acid region of the gpJ protein starting at positions 810-825 or 950-970 of the gpJ protein and ending at the C-terminal end of the gpJ protein. and the position is for the lambda bacteriophage gpJ protein sequence (SEQ ID NO: 10).

For the production of the chimeric gpJ protein, two insertion points (Fig. 7) were identified by the present inventors. In a non-limiting aspect, such an insertion site can be used for the production of a chimeric protein. Both insertion points yield functional gpJ chimeras with altered receptor binding. In one embodiment of the present invention, the bacterial delivery vehicle is a chimeric comprising a fusion between the N-terminal domain of a gpJ protein derived from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and the C-terminal domain of a different gpJ protein. contains a gpJ protein, wherein the N-terminal domain of the chimeric gpJ protein is in an amino acid region selected from positions 810-825, or 950-970 of the N-terminal domain with respect to the lambda bacteriophage gpJ protein sequence (SEQ ID NO: 10). It is fused to the C-terminal domain of a different gpJ protein within one.

In certain embodiments, the chimeric gpJ protein comprises a fusion between the N-terminal domain of a lambda bacteriophage gpJ protein and the C-terminal domain of a gpJ protein from a different bacteriophage, which typically recognizes and binds OmpC, said N-terminal The domain is fused to said C-terminal domain in the amino acid region 950-970 of the N-terminal domain, in particular for the lambda bacteriophage gpJ protein sequence (SEQ ID NO: 10). In this embodiment, the chimeric gpJ variant comprises or consists of the amino acid sequence SEQ ID NO: 11 and may be H591, typically encoded by the nucleotide sequence SEQ ID NO: 25, wherein the H591 chimeric gpJ variant typically OmpC recognize and combine In another embodiment, the chimeric gpJ protein comprises an N-terminal domain of a lambda bacteriophage gpJ protein, and typically E. It comprises a fusion between the C-terminal domains of gpJ proteins from different bacteriophages, recognizing a receptor present in the E. coli O157 strain, said N-terminal domain in particular N- to the lambda bacteriophage gpJ protein sequence (SEQ ID NO: 10). It is fused to said C-terminal domain in amino acid region 810-825 of the terminal domain. In this embodiment, the chimeric gpJ variant may be Z2145 comprising or consisting of the amino acid sequence SEQ ID NO: 12, typically encoded by the nucleotide sequence SEQ ID NO: 26, wherein the Z2145 chimeric gpJ variant typically Recognizes receptors present in strain O157. In still other embodiments, the chimeric gpJ protein comprises a fusion between the N-terminal domain of a lambda bacteriophage gpJ protein and the C-terminal domain of a gpJ protein from a different bacteriophage, which recognizes the Ompc receptor typically present in the O157 strain, , said N-terminal domain is fused to said C-terminal domain in amino acid region 950-970 of the N-terminal domain, in particular for the lambda bacteriophage gpJ protein sequence (SEQ ID NO: 10). In this embodiment, the chimeric gpJ variant may be 1A2 comprising or consisting of the amino acid sequence SEQ ID NO: 13, typically encoded by the nucleotide sequence SEQ ID NO: 27, wherein the 1A2 chimeric gpJ variant typically this. It recognizes the OmpC receptor present in the E. coli O157 strain. In another embodiment, the chimeric gpJ protein is typically the N-terminal domain of the lambda bacteriophage gpJ protein, and the C-terminus of the gpJ protein from a different bacteriophage, which typically recognizes the OmpC receptor present in both the O157 and MG1655 strains. inter-domain fusions, wherein said N-terminal domain is fused to said C-terminal domain within amino acid region 950-970 of the N-terminal domain, in particular for the lambda bacteriophage gpJ protein sequence (SEQ ID NO: 10). In this embodiment, the chimeric gpJ variant may be A8 comprising or consisting of the amino acid sequence SEQ ID NO: 49 and typically encoded by the nucleotide sequence SEQ ID NO: 55, wherein the A8 chimeric gpJ variant typically comprises this . It recognizes the OmpC receptor of both E. coli O157 and MG1655 strains.

Bacterial delivery vehicles comprising the recombinant gpH protein are also provided. Such gpH proteins include recombinant gpH proteins that allow or allow improved entry of bacterial vectors in cells having a defect or alteration in the permease complex. The recombinant engineered chimeric gpH protein may comprise a fusion between a portion of a gpH protein derived from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and a portion of a gpH protein derived from a corresponding gpH protein derived from a different bacteriophage. have. Such chimeric gpH proteins may comprise a fusion between the N-terminal domain of a gpH protein from a lambda-type bacteriophage, preferably a lambda or lambda-like bacteriophage, and the C-terminal domain of a different gpH protein. One such variant is gpH-IAI of the amino acid sequence SEQ ID NO: 24 and the nucleotide sequence SEQ ID NO: 28.

In certain embodiments, the bacterial delivery vehicle comprises a chimeric STF-V10h variant as disclosed above and a chimeric 1A2 variant as disclosed above.

In an aspect, the bacterial delivery vehicle provided herein comprises a recombinant STF protein(s) (including, but not limited to, chimeric and branched RBPs) engineered to increase the efficiency of delivery of a DNA payload to a targeted bacterial cell population, gpJ protein vehicle comprising (s) and/or gpH protein(s). Such bacterial cell populations include, for example, E. coli and other bacterial species of interest.

It has also been demonstrated herein that the size of the packaged genome can have an effect on packaging efficiency.

Nucleic acid molecules encoding the wild-type as well as recombinant STF, gpJ, and gpH proteins disclosed herein are provided. Such nucleic acids can be included in vectors such as bacteriophages, plasmids, phagemids, viruses, and other vehicles that allow for the delivery and expression of recombinant STF, gpJ, and gpH encoding nucleic acids.

In certain embodiments, the nucleic acid is comprised in a single vector. In a more specific embodiment, the vector comprises or consists of the nucleic acid sequence SEQ ID NO: 47.

Bacterial delivery vehicles provided herein allow for delivery of a nucleic acid payload encoding a protein or nucleic acid of interest to a desired target bacterial host cell. As used herein, the term “delivery vehicle” means any means that permits delivery of a payload to a bacterium. Without limitation, bacteriophage scaffolds, viral scaffolds, chemical based delivery vehicles (eg, cyclodextrins, calcium phosphate, cationic polymers, cationic liposomes), protein-based or peptide-based delivery vehicles, lipid-based delivery vehicles , nanoparticle-based delivery vehicles, non-chemical-based delivery vehicles (eg, transformation, electroporation, sonoporation, optical transfection), particle-based delivery vehicles (eg, gene gun, magnetofection) There are several types of delivery vehicles encompassed by this disclosure, including , impalofection, particle bombardment, cell-penetrating peptides) or donor bacteria (conjugated). Any combination of delivery vehicles is also encompassed by the present disclosure. Delivery vehicle may refer to a bacteriophage derived scaffold and may be obtained from a natural, evolved or engineered capsid.

Delivery vehicles include packaged phagemids as well as bacteriophages, as disclosed herein. eligobiotic® is a payload encapsidated in a packaged phagemid, ie, a phage-derived capsid. The manipulation of such delivery vehicles is well known to those skilled in the art. These engineering techniques can be applied to production cell lines engineered to express the STF, gpJ and gpH proteins disclosed herein. In one aspect, a bacterial delivery vehicle having a desired target host range is provided for use in the delivery of a payload to a microbiome of a host. Bacterial delivery vehicles can be characterized by a combination of wild-type and recombinant STF, gpJ and gpH proteins.

The present disclosure also provides production cell lines for producing the bacterial delivery vehicles disclosed herein.

The production of packaged phagemids and bacteriophage particles is a conventional technique well known to those skilled in the art. In one embodiment, satellite phage and/or helper phage may be used to facilitate packaging of payloads in the delivery vehicles disclosed herein. Helper phages serve their function in trans and are well known to those skilled in the art. Helper phage includes all genes encoding structural and functional proteins essential for the payload being packaged (ie, helper phage provides all essential gene products for assembly of the delivery vehicle). The helper phage may contain a defective origin of replication or packaging signal, or the latter may be completely absent and thus unable to self-package, thus producing only bacterial transfer particles carrying a payload or plasmid. Helper phages may be selected such that they cannot induce lysis of the host used to produce the delivery particle. Those skilled in the art understand that some bacteriophages are defective and require helper phages for payload packaging. Thus, depending on the bacteriophage selected for making the bacterial delivery particle, one of ordinary skill in the art will know whether a helper phage is needed. Sequences encoding one or more proteins or regulatory processes required for assembly or production of a packaged payload may be supplied in trans. For example, the STF, gpJ and gpH proteins of the present disclosure can be constitutively expressed or provided on a plasmid under the control of an inducible promoter. In this case, the phage wild-type sequence may or may not contain a deletion of the gene or sequence supplied in trans. In addition, chimeric or modified phage sequences encoding new functions, such as recombinant STF, gpJ or gpH proteins, can be inserted directly into the desired location in the genome of the helper phage, thus eliminating the need to provide the modified sequence in trans. turn around Methods for supplying sequences or proteins in trans in the form of plasmids, as well as methods for direct genomic insertions, modifications and mutations are well known to those skilled in the art. In certain embodiments, the production cell line produces:

- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 14 and an associated chaperone thereof comprising or consisting of the amino acid sequence of SEQ ID NO: 15,

- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 16,

- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 17 and an associated chaperone thereof comprising or consisting of the amino acid sequence of SEQ ID NO: 18,

- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 19 and an associated chaperone thereof comprising or consisting of the amino acid sequence of EQ ID NO: 20,

- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 21 and an associated chaperone thereof comprising or consisting of the amino acid sequence of SEQ ID NO: 22,

- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 44, or

- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 50 and optionally an associated chaperone thereof comprising or consisting of the amino acid sequence of SEQ ID NO: 57.

In certain embodiments, the helper phage comprises a nucleic acid sequence encoding a chimeric RBP comprising or consisting of the sequence SEQ ID NO: 48, wherein the nucleic acid sequence typically comprises or consists of the sequence SEQ ID NO: 54 and the helper phage optionally further comprises a nucleic acid sequence encoding a chimeric gpJ variant comprising or consisting of the sequence SEQ ID NO: 13, wherein the nucleic acid sequence typically comprises or consists of the sequence SEQ ID NO: 27 . In certain embodiments, the helper phage is a lambda phage, and (i) the nucleic acid encoding the wild-type STF protein is substituted by a nucleic acid sequence encoding a chimeric RBP comprising or consisting of the sequence SEQ ID NO: 48, wherein the nucleic acid The sequence typically comprises or consists of the sequence SEQ ID NO: 54, and (ii) the nucleic acid encoding the wild-type gpJ protein comprises or consists of the sequence SEQ ID NO: 13 in the nucleic acid sequence encoding the chimeric gpJ variant wherein the nucleic acid sequence typically comprises or consists of the sequence SEQ ID NO: 27, (iii) the Cos site has been removed, and optionally (iv) the helper prophage is a mutation that prevents spontaneous cell lysis, such as contains the Sam7 mutation, and (v) the helper prophage contains a thermosensitive form of the master cI repressor, such as the cI857 form.

In one embodiment, the bacterial delivery vehicle disclosed herein comprises a DNA payload of interest. As used herein, the term “payload” refers to any nucleic acid sequence or amino acid sequence, or a combination of both (eg, without limitation, a peptide nucleic acid or a peptide-oligonucleotide conjugate), that is delivered to a bacterium by a delivery vehicle. do. The term “payload” may also be a plasmid, vector, or cargo. The payload may be a phasmid or phagemid obtained from a native, evolved or engineered bacteriophage genome. A payload may also be included in only a portion of a phagemid or phasmid obtained from a natural, evolved or engineered bacteriophage genome.

As shown in Example 1 below, the efficiency of loading a payload by a bacterial delivery vehicle disclosed herein may depend, inter alia, on the size of the payload. Thus, in certain embodiments, the payload has a size of at least 4 kb, preferably no greater than 51 kb.

In the above embodiment, the payload may have an integer multiple of 36 kb to 51 kb. In other words, in that embodiment, at least an integer n is present, such as 36 kb ≤ n x size of the payload ≤ 51 kb.

The inventors have demonstrated that it is possible to produce a more uniform population of bacterial delivery vehicles comprising a nearly unique number of payload copies, more particularly when the payload has a specific range of sizes.

In certain embodiments, the payload has a size strictly superior to 10.000 kb and strictly inferior to 12.000 kb. In an alternative embodiment, the payload has a size strictly superior to 12.500 kb and strictly inferior to 16.667 kb, in particular a size strictly superior to 12.500 kb and inferior to 13.000 kb.

In another specific embodiment, the payload has a size of at least 18.000 kb and no more than 25.000 kb, in particular no more than 24.000 kb.

The payload can be a nucleic acid plasmid that can be circularized upon delivery to a target cell and then replicated or integrated into a chromosome. Replication of vector DNA is dependent on the existence of a bacterial origin of replication. Once replicated, the inheritance of the plasmid into each daughter cell may be mediated by active division mechanisms and the presence of plasmid poisoning systems such as toxin/anti-toxin systems.

As used herein, the term “nucleic acid” refers to a sequence of at least two nucleotides covalently linked together that may be single-stranded or double-stranded or contain portions of both single-stranded and double-stranded sequences. Nucleic acids may be naturally occurring, recombinant or synthetic. A nucleic acid may be in the form of a circular sequence or a linear sequence, or a combination of both. A nucleic acid may be DNA, genomic or cDNA, or RNA or a combination of both. A nucleic acid can be any combination of deoxyribonucleotides and ribonucleotides, and any combination of bases, including uracil, adenine, thymine, cytosine, guanine, inosine, xanthine, hypoxanthine, isocytosine, 5-hydroxymethylcytosine and isoguanine. may contain Other examples of modified bases that may be used are detailed in Chemical Reviews 2016, 116 (20) 12655-12687. The term "nucleic acid" also contains, without limitation, phosphoramide, phosphorothioate, phosphorodithioate, O-methylphosphoroamidite linkages and/or other backbones including deoxyribonucleotides and ribonucleotide nucleic acids. It encompasses any nucleic acid analog capable of Any combination of the above characteristics of a nucleic acid is also encompassed by the present disclosure.

Origins of replication known in the art include species-specific plasmid DNA (e.g., CoIE1, Rl, pT181, pSC101, pMB1, R6K, RK2, p15a, etc.), bacterial viruses (e.g., φX174, M13, F1 and P4) and bacterial chromosomal origins of replication (eg, oriC). In one embodiment, a phagemid according to the present disclosure comprises a bacterial origin of replication that is functional in the targeted bacterium.

Alternatively, a plasmid according to the present disclosure does not contain any functional bacterial origin of replication or contains an inactive origin of replication in the targeted bacterium. Thus, the plasmids of the present disclosure cannot replicate themselves once introduced into bacteria by bacterial viral particles.

In one embodiment, the origin of replication on the plasmid to be packaged is inactive in the targeted bacterium, meaning that the origin of replication does not function in the bacterium targeted by the bacterial viral particle, thereby preventing unwanted plasmid replication.

In one embodiment, the plasmid comprises a bacterial origin of replication that is functional in the bacteria used for the production of bacterial viral particles.

Plasmid replication relies on host enzymes and plasmid-controlled cis and trans determinants. For example, some plasmids have determinants that are recognized by almost all Gram-negative bacteria and function correctly in each host during replication initiation and regulation. Other plasmids retain this ability only in some bacteria (Kues, U and Stahl, U 1989 Microbiol Rev 53:491-516).

Plasmids are replicated by three general mechanisms, originating in replication origin: theta type, strand displacement, and rolling circles (Del Solar et al. 1998 Microhio and Molec Biol. Rev 62:434-464). These origins of replication contain sites necessary for the interaction of host-encoded proteins and/or plasmids.

The origin of replication used on the plasmids of the present disclosure may be medium copy number, pBR322 (15-20 copies per cell) or R6K plasmid (15-20 copies per cell) derived such as colEl ori, or high copy number, e.g. For example, pUC oris (500-700 copies per cell), pGEM oris (300-400 copies per cell), pTZ oris (>1000 copies per cell) or pBluescript oris (300-500 copies per cell) can

In one embodiment, the bacterial origin of replication is ColE1, pMB1 and variants (pBR322, pET, pUC, etc.), p15a, ColA, ColE2, pOSAK, pSC101, R6K, IncW (pSa, etc.), IncFII, pT181, P1, F IncP, IncC, IncJ, IncN, IncP1, IncP4, IncQ, IncH11, RSF1010, CloDF13, NTP16, R1, f5, pPS10, pC194, pE194, BBR1, pBC1, pEP2, pWVO1, pLF1311, pAP1, pWKS1, pLS1, pWKS11, pUB606060LS11, pUB pJD4, pIJ101, pSN22, pAMbeta1, pIP501, pIP407, ZM6100(Sa), pCU1, RA3, pMOL98, RK2/RP4/RP1/R68, pB10, R300B, pRO1614, pRO1600, pECB2, pCM1, pFA3, RepFIA, RepFIA , RepFIC, pYVE439-80, R387, Parsil, RA1, TF-FC2, pMV158 and pUB113.

In one embodiment, the bacterial origin of replication is ColE1, pMB1 and variants (pBR322, pET, pUC, etc.), p15a, ColA, ColE2, pOSAK, pSC101, R6K, IncW (pSa, etc.), IncFII, pT181, P1, F IncP, E. selected from the group consisting of IncC, IncJ, IncN, IncP1, IncP4, IncQ, IncH11, RSF1010, CloDF13, NTP16, R1, f5 and pPS10. Coke is of clone origin.

In one embodiment, the bacterial origin of replication is pC194, pE194, BBR1, pBC1, pEP2, pWVO1, pLF1311, pAP1, pWKS1, pLS1, pLS11, pUB6060, pJD4, pIJ101, pSN22, pAMbeta1, pIP501, pIP407, ZM6100 (Sa ), pCU1, RA3, pMOL98, RK2/RP4/RP1/R68, pB10, R300B, pRO1614, pRO1600, pECB2, pCM1, pFA3, RepFIA, RepFIB, RepFIC, pYVE439-80, R387, Parsil, RA1, TF-FC2, pMV158 and pUB113.

In one embodiment, the bacterial origin of replication is ColE1.

A nucleic acid sequence delivered in accordance with the present disclosure may comprise an origin of phage replication, capable of initiating replication of the transferred nucleic acid sequence for subsequent encapsulation into a different capsid, with the complementarity of the complete phage genome.

The phage origin of replication comprised in the delivered nucleic acid sequences of the present disclosure may be any origin of replication found in phage.

In one embodiment, the origin of phage replication is M13, f1, φX174, P4, lambda, P2, lambda-like, HK022, mEP237, HK97, HK629, HK630, mEP043, mEP213, mEP234, mEP390, mEP460, mEPx1, mEPx2, phi80EP , mEP234, T2, T4, T5, T7, RB49, phiX174, R17, PRD1 Pl-like, P2-like, P22, P22-like, N15 and N15-like bacteriophage wild-type or non-wild-type sequences.

In one embodiment, the phage origin of replication is selected from the group consisting of M13, f1, φX174, P4, and the phage origin of replication in lambda.

In certain embodiments, the phage origin of replication is a lambda or P4 origin.

The nucleic acid of interest to be delivered comprises a nucleic acid sequence under the control of a promoter. In certain embodiments, the nucleic acid of interest is a Cas nuclease gene, Cas9 nuclease gene, guide RNA, CRISPR locus, toxin gene, enzyme such as a nuclease or kinase, TALEN, ZFN, a meganuclease, recombinase , a gene encoding a bacterial receptor, a membrane protein, a structural protein, a secreted protein, a gene encoding resistance to an antibiotic or generic drug, a gene encoding a toxic protein or a virulence factor, and a gene encoding a virulence protein or a virulence factor , or any combination thereof. In one embodiment, the nucleic acid payload encodes a therapeutic protein. In another embodiment, the nucleic acid payload encodes an antisense nucleic acid molecule.

In one embodiment, the sequence of interest is a programmable nuclease cycle for delivery to a targeted bacterium. Such programmable nuclease circuitry can mediate in vivo sequence-specific clearance of bacteria containing a target gene of interest (eg, a gene deleterious to humans). Some embodiments of the present disclosure include Streptococcus pyogenes ( Streptococcus pyogenes ) to engineered variants of the type II CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-associated) system. Other programmable nucleases that can be used include other CRISPR-Cas systems, engineered TALEN (Transcription Activator-Like Effector Nuclease) variants, engineered zinc finger nuclease (ZFN) variants, natural, evolved or engineered meganucleases. agents or recombinase variants, and any combination or hybrid of programmable nucleases. Thus, the engineered self-distributed nuclease circuit provided herein encodes a gene of interest such as, for example, a toxin gene, a virulence factor gene, an antibiotic resistance gene, a remodeling gene or a regulatory gene (see WO2014124226). It can be used to selectively cut DNA.

Other sequences of interest, such as programmable sequences, can be added to the delivered nucleic acid sequence for delivery to the targeted bacterium. In one embodiment, the sequence of interest added to the delivered nucleic acid sequence causes cell death of the targeted bacterium. For example, the nucleic acid sequence of interest added to the plasmid may encode a holine or toxin.

Alternatively, the sequence of the circuit of interest added to the transferred nucleic acid sequence does not cause bacterial death. For example, the sequence of interest may encode a reporter gene that elicits a luminescent or fluorescent signal. Alternatively, sequences of interest may include proteins and enzymes that have acquired useful functions such as alteration of the bacterium's metabolism or its environmental composition.

In certain embodiments, the nucleic acid sequence of interest is Cas9, single guide RNA (sgRNA), CRISPR locus, enzymes such as nucleases or kinases, TALENs, ZFNs, meganucleases, recombinases, bacterial receptors, membrane proteins, structural proteins , a gene encoding a secreted protein, a gene encoding resistance to antibiotics or generic drugs, a gene encoding a toxic protein or virulence factor, and a gene encoding a virulence protein or virulence factor.

In certain embodiments, the nucleic acid sequence delivered according to the present disclosure comprises a nucleic acid sequence of interest encoding a bacteriocin, which may be a proteinaceous toxin produced by a bacterium to inhibit the growth or kill of another bacterium. Bacteriocins are classified in several ways, including production strains, common resistance mechanisms, and death mechanisms. Such bacteriocins have been described from gram-negative bacteria (eg, microcins, colicin-like bacteriocins and tailosin) and gram-positive bacteria (eg, class I, class II, class III or class IV bacteriocins).

In one embodiment, the nucleic acid sequence delivered according to the present disclosure is of interest encoding a toxin selected from the group consisting of microcins, colicin-like bacteriocins, tailosin, class I, class II, class III and class IV bacteriocins. further comprising sequences.

In certain embodiments, the corresponding immune polypeptide (i.e., anti-toxin) can be used to protect bacterial cells for delivery nucleic acid sequence production and encapsidation purposes (Cotter et al., Nature Reviews Microbiology 11: 95, 2013, which is incorporated herein by reference in its entirety), is absent in targeted bacteria and pharmaceutical compositions to which the delivered nucleic acid sequences of the present disclosure are delivered.

In one aspect of the present disclosure, a CRISPR system is comprised in the delivered nucleic acid sequence. The CRISPR system contains two distinct components: i) an endonuclease, in this case a CRISPR-associated nuclease (Cas or "CRISPR-associated protein") and ii) a guide RNA. Guide RNA is a form of chimeric RNA consisting of a combination of CRISPR (RNAcr) bacterial RNA and RNAtracr (trans-activating RNA CRISPR) (Jinek et al., Science 2012). The guide RNA combines the targeting specificity of RNAcr corresponding to a “spacing sequence” that serves as a guide to the Cas protein, and the conformational nature of RNAtracr into a single transcript. When the guide RNA and Cas protein are expressed simultaneously in a cell, the target genomic sequence may be permanently modified or blocked. The deformation is advantageously guided by the repair matrix. In general, the CRISPR system comprises two major classes depending on the mechanism of action of the nuclease. Class 1 is made up of multiple-subunit effector complexes and includes types I, III and IV. Class 2 is made of single-unit effector modules, such as Cas9 nucleases, type II (II-A,II-B,II-C,II-C variants), type V (V-A,V-B,V-C,V-D,V-E) , V-U1, V-U2, V-U3, V-U4, V-U5) and Type VI (VI-A, VI-B1, VI-B2, VI-C, VI-D).

A sequence of interest according to the present disclosure comprises a nucleic acid sequence encoding a Cas protein. A variety of CRISPR enzymes are available for use as sequences of interest on plasmids. In certain embodiments, the CRISPR enzyme is a type II CRISPR enzyme. In certain embodiments, the CRISPR enzyme catalyzes DNA cleavage. In some other embodiments, the CRISPR enzyme catalyzes RNA cleavage. In one embodiment, the CRISPR enzyme may be coupled to an sgRNA. In certain embodiments, the sgRNA comprises an antibiotic resistance gene, a virulence protein or factor gene, a toxin protein or factor gene, a bacterial receptor gene, a membrane protein gene, a structural protein gene, a secreted protein gene, and a gene encoding resistance to an over-the-counter drug. Target a gene selected from the group.

Non-limiting examples of Cas proteins as part of a multi-subunit effector or single-unit effector include Cas1, Cas1B, Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9 (also known as Csn1 and Csx12), Cas10, Cas11 (SS), Cas12a (Cpf1), Cas12b (C2c1), Cas12c (C2c3), Cas12d (CasY), Cas12e (CasX), C2c4, C2c8, C2c5, C2c10, C2c9, Cas13a (C2c2), Cas13b (C2c6) Cas13c (C2c7), Cas13d, Csa5, Csc1, Csc2, Cse1, Cse2, Csy1, Csy2, Csy3, Csf1, Csf2, Csf3, Csf4, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr , Csn2, Csb1, Csb2, Csb3, Csx17, Csx14, Csx10, Csx16, CsaX, Csx13, Csx1, Csx15, SdCpf1, CmtCpf1, TsCpf1, TsCpf1, FbCpf1, ErCpbCpf1, ErCpcpf1, PcCpff1, PcCpff1, PcCpf orthologs, variants thereof, or modified forms thereof. In certain embodiments, the CRISPR enzyme cleaves both strands of the target nucleic acid at the Protospacer Adjacent Motif (PAM) site.

In certain embodiments, the CRISPR enzyme is any Cas9 protein, eg, any naturally occurring bacterial Cas9 as well as any variant, homolog or ortholog thereof.

"Cas9" means the protein Cas9 (Csn1 or Csx12) or It refers to a functional protein, peptide or polypeptide fragment thereof. Thus, "Cas9" can mean a modified protein, e.g., truncated to remove domains of the protein that are not essential for the predefined function of the protein, in particular domains that are not essential for interaction with the gRNA(s). .

The sequence encoding Cas9 (whole protein or fragment thereof) used in the context of the present disclosure can be obtained from any known Cas9 protein (Fonfara et al., Nucleic Acids Res 42 (4), 2014; Koonin et al. , Nat Rev Microbiol 15(3), 2017). Examples of Cas9 proteins useful in the present disclosure include, without limitation, Streptococcus pyogenes (SpCas9), Streptococcus thermophiles (St1Cas9, St3Cas9), Streptococcus mutans ( Streptococcus mutans ), Staphylococcus aureus ) (SaCas9), Campylobacter jejuni (CjCas9), Francisella novicida ( Francisella novicida ) (FnCas9) and the Cas9 protein of Neisseria meningitides (NmCas9).

The sequence encoding Cpf1 (Cas12a) (whole protein or fragment thereof) used in the context of the present disclosure can be obtained from any known Cpf1 (Cas12a) protein (Koonin et al., 2017). Examples of Cpf1 (Cas12a) proteins useful in the present disclosure include, without limitation, Acidaminococcus sp , Lachnospiraceae bacteriu and Francisella novicida of Cpf1 ( Cas12a) protein.

The sequence encoding Cas13a (whole protein or fragment thereof) can be obtained from any known Cas13a (C2c2) protein (Abudayyeh et al., 2017). Examples of Cas13a (C2c2) proteins useful in the present disclosure include, without limitation, the Cas13a (C2c2) protein of Leptotrichia wadei ( LwaCas13a ).

The sequence encoding Cas13d (whole protein or fragment thereof) can be obtained from any known Cas13d protein (Yan et al., 2018). Examples of Cas13d proteins useful in the present disclosure include, without limitation, Cas13d proteins of Eubacterium siraeum and Ruminococcus sp .

In certain embodiments, the nucleic acid sequence of interest is an antibiotic resistance gene, a virulence factor or protein gene, a toxin factor or protein gene, a bacterial receptor, a membrane protein, a structural protein, a gene encoding a secreted protein, and a gene encoding resistance to a generic drug A CRISPR/Cas9 system for inactivation or reduction of gene expression of a gene selected from the group consisting of genes.

In one embodiment, the CRISPR system is used to target and inactivate a virulence factor. A virulence factor can be any substance produced by a pathogen that alters the host-pathogen interaction by increasing the degree of damage inflicted on the host. Virulence factors include, for example, in cell adhesion or colonization of niche within the host, evading the host's immune response, promoting entry into and excretion from host cells, obtaining nutrients from the host, or inhibiting other physiological processes in the host. Thus, it is used by pathogens in many ways. The virulence factors may include enzymes, endotoxins, adhesion factors, motility factors, factors involved in complement evasion, and biofilm formation promoting factors. For example, such a targeted virulence factor gene is E. E. coli virulence factor genes such as, but not limited to, EHEC-HlyA, Stx1 (VT1), Stx2 (VT2), Stx2a (VT2a), Stx2b (VT2b), Stx2c (VT2c), Stx2d (VT2d), Stx2e (VT2e) and Stx2f ( VT2f), Stx2h (VT2h), fimA, fimF, fimH, neuC, kpsE, sfa, foc, iroN, aer, iha, papC, papGI, papGII, papGIII, hlyC, cnf1, hra, sat, ireA, usp ompT, ibeA , malX, fyuA, irp2, traT, afaD, ipaH, eltB, estA, bfpA, eaeA, espA, aaiC, aatA, TEM, CTX, SHV, csgA, csgB, csgC, csgD, csgE, csgF, csSS , T2SS, T3SS, T4SS, T5SS, T6SS (secretory system). For example, such a targeted virulence factor gene can be a Shigella dysenteriae virulence factor gene such as, without limitation, stx1 and stx2. For example, such a targeted virulence factor gene can be a Yersinia pestis virulence factor gene such as, without limitation, yscF (plasmid-derived (pCD1) T3SS outer needle-like subunit). For example, such a targeted virulence factor gene may be a Francisella tularensis virulence factor gene such as, but not limited to, fslA. For example, such a targeted virulence factor gene may be a Bacillus anthracis virulence factor gene such as, without limitation, pag (anthrax toxin, cell-associated protective antigen). For example, such targeted virulence factor genes can be Vibrio cholera virulence factor genes such as, without limitation, ctxA and ctxB (cholera toxin), tcpA (toxin coregulatory cilia), and toxT (master virulence regulator). can For example, such a targeted virulence factor gene may be a Pseudomonas aeruginosa virulence factor gene such as, but not limited to, fioberdin (eg, sigma factor pvdS, biosynthetic genes pvdL, pvdl, pvdJ, pvdH, pvdA, pvdF, pvdQ, pvdN, pvdM, pvdO, pvdP, transporter genes pvdE, pvdR, pvdT, opmQ), siderophore piochelin (e.g., pchD, pchC, pchB, pchA, pchE, pchF and pchG, and toxins) (eg exoU, exoS and exoT).For example, this targeted virulence factor gene is a Klebsiella pneumoniae virulence factor gene such as, but not limited to, fimA (attachment, I type fimbria major subunit), and cps (capsule polysaccharide).For example, this targeted virulence factor gene is Acinetobacter baumannii virulence factor gene such as, without limitation, ptk (capsule polymerization). ) and epsA (assembled).For example, this targeted virulence factor gene is Salmonella enterica Tipi virulence factor genes such as those associated with bile tolerance, including, without limitation, MIA (invasion, SPI-1 regulator), ssrB (SPI-2 regulator), and the efflux pump genes acrA, acrB and tolC. For example, such a targeted virulence factor gene can be a Fusobacterium nucleatum virulence factor gene such as, without limitation, FadA and TIGIT. For example, such a targeted virulence factor gene can be a Bacteroides fragilis virulence factor gene such as, but not limited to, bft.

In other embodiments, the CRISPR/Cas9 system comprises antibiotic resistance genes such as, but not limited to, GyrB, ParE, ParY, AAC(1), AAC(2'), AAC(3), AAC(6'), ANT(2" ), ANT(3"), ANT(4'), ANT(6), ANT(9), APH(2"), APH(3"), APH(3'), APH(4), APH(6 ), APH(7"), APH(9), ArmA, RmtA, RmtB, RmtC, Sgm, AER, BLA1, CTX-M, KPC, SHV, TEM, BlaB, CcrA, IMP, NDM, VIM, ACT, AmpC , CMY, LAT, PDC, OXA β-lactamase, mecA, Omp36, OmpF, PIB, bla (blaI, blaR1) and mec (mecI, mecR1) operons, chloramphenicol acetyltransferase (CAT), chloramphenicol phosphotransferase, ethambutol-resistant arabinosyltransferase (EmbB), MupA, MupB, integral membrane protein MprF, Cfr 23S rRNA methyltransferase, rifampin ADP-ribosyltransferase (Arr), rifampin glycosyltransferase, rifampin monooxygenase, Rifampin phosphotransferase, DnaA, RbpA, rifampin-resistant-beta-subunit of RNA polymerase (RpoB), Erm 23S rRNA methyltransferase, Lsa, MsrA, Vga, VgaB, streptogramin Vgb lyase, Vat acetyltransferase Lase, fluoroquinolone acetyltransferase, fluoroquinolone-resistant DNA topoisomerase, fluoroquinolone-resistant GyrA, GyrB, ParC, quinolone resistance protein (Qnr), FomA, FomB, FosC, FosA, FosB, FosX, VanA, VanB, VanD, VanR, VanS, lincosamide nucleotidyltransferase (Lin), EreA, EreB, GimA, Mgt, Ole, macrolide phosphotransferase (MPH), MefA, MefE, Mel, strepto Tricine acetyltransferase (sat), Sul1, Sul2, Sul3 , sulfonamide-resistant FolP, tetracycline inactivating enzymes TetX, TetA, TetB, TetC, Tet30, Tet31, TetM, TetO, TetQ, Tet32, Tet36, MacAB-TolC, MsbA, MsrA,VgaB, EmrD, EmrAB-TolC, NorB, GepA, MepA, AdeABC, AcrD, MexAB-OprM, mtrCDE, EmrE, adeR, acrR, baeSR, mexR, phoPQ, mtrR, or Comprehensive Antibiotic Resistance Database (CARD https://card.mcmaster .ca/) to target and inactivate any antibiotic resistance gene.

In another embodiment, the CRISPR/Cas9 system is used to target and inactivate bacterial toxin genes. Bacterial toxins can be classified as exotoxins or endotoxins. Exotoxins are produced and actively secreted; Endotoxins remain part of the bacteria. Responses to bacterial toxins can include severe inflammation and can lead to sepsis. Such toxins include, for example, botulinum neurotoxin, tetanus, toxin, staphylococcal toxin, diphtheria toxin, anthrax toxin, alpha toxin, pertussis toxin, Shiga toxin, heat-stable enterotoxin (E. coli ST), colibactin , BFT (B. fragilis toxin) or any toxin described in Henkel et al., (Toxins from Bacteria in EXS. 2010; 100: 1-29).

In certain embodiments, the payload comprises or consists of the nucleic acid sequence SEQ ID NO: 47.

The bacteria targeted by the bacterial delivery vehicles disclosed herein can be any bacteria present in a mammalian organism. In certain aspects, the bacteria are targeted through interaction of the bacterial cells with a chimeric RBP and/or a divergent-RBP expressed by the delivery vehicle. It may be any commensal, commensal, or pathogenic bacterium of a microbiota or microbiome.

The microbiome may include a variety of endogenous bacterial species, any of which may be targeted in accordance with the present disclosure. In certain embodiments, the genus and/or species of endogenous bacterial cells targeted may depend on the type of bacteriophage used to prepare the bacterial delivery vehicle. For example, some bacteriophages show tropism or preferentially target a particular host species of bacteria. Other bacteriophages do not exhibit such tropism and can be used to target endogenous bacterial cells of many different genera and/or species.

Examples of bacterial cells include, without limitation, the genera Yersinia spp., Escherichia spp., Klebsiella spp., Acinetobacter spp., Bordetella . spp., Neisseria spp., Aeromonas spp., Franciesella spp., Corynebacterium spp ., Citrobacter spp., Chlamydia spp . ., Haemophilus spp., Brucella spp., Mycobacterium spp., Legionella spp., Rhodococcus spp., Pseudomonas spp. , Helicobacter spp., Vibrio spp., Bacillus spp., Erysipelothrix spp., Salmonella spp., Streptomyces spp., Streptomyces spp. Cus ( Streptococcus ) spp., Staphylococcus spp., Bacteroides spp., Prevotella spp., Clostridium spp., Bifidobacterium ) spp., Clostridium spp., Brevibacterium spp., Lactococcus spp., Leuconostoc spp., Actinobacillus spp., Selnomonas ( Selnomonas ) spp., Shigella spp., Zymonas spp., Mycoplasma spp., Treponema spp ., Leukonostok spp. , Corynebacterium spp . ., Enterococcus spp., Enterobacter spp., Pyrococcus spp., Serratia spp., Morganasella spp., Parvimonas ( Parvimonas ) spp. ) spp., Fusobacterium spp., Actinomyces spp., Porphyromonas spp., Micrococcus spp., Bartonella spp. , Borrelia spp., Brucelia spp., Campylobacter spp., Chlamydophilia spp., Cutibacterium (formerly Propionibacterium ) spp., Ehrlichia spp., Haemophilus spp., Leptospira spp., Listeria spp., Mycoplasma spp., Nocardia spp., Rickettsia ( Rickettsia ) spp., Ureaplasma spp ., and Lactobacillus spp ., and cells derived from bacteria of mixtures thereof.

Accordingly, a bacterial delivery vehicle may target (eg, specifically target) bacterial cells from any one or more of the aforementioned genus bacteria to specifically deliver a payload of interest in accordance with the present disclosure.

In one embodiment, the targeted bacterium is Yersinia spp., Escherichia spp., Klebsiella spp., Acinetobacter spp., Pseudomonas spp., Helicobacter spp., Vibrio spp, Salmonella spp., Streptococcus spp., Staphylococcus spp., Bacteroides spp., Clostridium spp., Shigella spp., Enterococcus spp., Enterobacter spp., and Listeria spp. It can be selected from the group consisting of.

In certain embodiments, the targeted bacterial cells of the present disclosure are anaerobic bacterial cells (eg, cells that do not require oxygen for growth). Anaerobic bacterial cells include, but are not limited to, conditionally anaerobic cells such as Escherichia cli , Shewanella oneidensis and Listeria . Anaerobic bacterial cells include obligate anaerobic cells such as, for example, Bacteroides and Clostridial species. In humans, anaerobic bacteria are most commonly present in the gastrointestinal tract. In some specific embodiments, the targeted bacterium is thus the bacterium most commonly present in the gastrointestinal tract. Bacteriophages used to prepare bacterial viral particles, and then bacterial viral particles, target (e.g., specifically target )can do.

In certain embodiments, the targeted bacterial cell is, without limitation, Bacteroides thetaiotaomicron , Bacteroides fragilis , Bacteroides distasonis , Bacteroys Des vulgatus ( Bacteroides vulgatus ), Clostridium leptum , Clostridium coccoides , Staphylococcus aureus , Bacillus subtilis , Clost Lidium butyricum ( Clostridium butyricum ), Brevibacterium lactofermentum ( Brevibacterium lactofermentum ), Streptococcus agalactiae ), Lactococcus lactis ( Lactococcus lactis ), Leuconostok lactis ), Actinobacillus actinobycetemcomitans ( Actinobacillus actinobycetemcomitans ), cyanobacteria ( cyanobacteria ), Escherichia coli ( Escherichia coli ), Helicobacter pylori ( Helicobacter pylori ), Selnomonas ruminas rum ( Selnomonas ruminas ) Shigella sonnei , Zymomonas mobilis , Mycoplasma mycoides , Treponema denticola , Bacillus thuringiensis , Caucus lugdunensis ( Staphilococcus lugdunensis ), Leuconostoc oenos ( Leuconostoc oe ) nos ), Corynebacterium zero sis ( Corynebacterium xerosis ), Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus acidophil Lactobacillus ( Lactobacillus casei ), Lactobacillus casei ), Enterococcus faecalis , Bacillus coagulans , Bacillus cereus , Bacillus popillae , Synechocystis , Bacillus lice strain PCC680 Sien ( Bacillus liquefaciens ), Pyrococcus abyssi ), Selenomonas nominantium , Lactobacillus hilgardii ), Streptococcus ferus ( Streptococcus ferrus ) Pentosus ( Lactobacillus pentosus ), Bacteroides fragilis ), Staphylococcus epidermidis , Streptomyces phaechromogenes ) , Klebsiella pneumoniae , Enterobacter cloacae , Enterobacter aerogenes , Serratia marcescens , Morganella morganii ), Citrobacter pruundi ( Citrobacter freundii ), Propionibacterium freudenreichii ), Pseudomonas aeruginosa ( Pseudomonas aeruginosa ), Parvimonas micra ( Parvimonas micra ), Prevotella intermedia ( Prevotella intermedia ) Rium nucleatum ( Fusobacterium nucleatum ), Prevotella nigrescens , Actinomyces israelii , Porphyromonas endodontalis , Porphyromonas endodontalis , Porphyromonas gingivalis gingivalis ), Micrococcus luteus , Bacillus megaterium , Aeromonas hydrophila , Aeromonas caviae , Bacillus anthracis , Bacillus anthracis Tonella henselae ( Bartonella henselae ), Bartonella Quintana ( Bartonella Quintana ), Bordetella pertussis ( Bordetella pertussis ), Borrelia burgdorferi , Borrelia garinii , Borrelia Afzelii ( Borrelia afzelii ), Borrelia recurrentis ( Borrelia recurrentis ), Brucella abortus ( Brucella abortus ), Brucella canis ( Brucella canis ), Brucella melitensis ( Brucella melitensis ), Brucella suis ), Campylobacter jejuni , Campylobacter Coli ( Campylobacter coli ), Campylobacter fetus ( Campylobacter fetus ), Chlamydia pneumoniae ( Chlamydia pneumoniae ), Chlamydia trachomatis ( Chlamydia trachomatis ), Chlamydophila psittaci , Clostridium ( Chlamydophila psittaci ) Clostridium botulinum ), Clostridium difficile , Clostridium perfringens , Clostridium tetani , Corynebacterium diphtheria , Cutibacterium acnes ( Cutibacterium acnes ) (formerly Propionibacterium acnes ), Ehrlichia canis , Ehrlichia chaffeensis , Enterococcus faecium , Francisella tula Lensis ( Francisella tularensis ), Haemophilus influenzae ( Haemophilus influen ), Legionella pneumophila ( Legionella pneumophila ), Leptospira interrogans ( Leptospira interrogans ), Leptospira santa rosai ( Leptospira santarosai ), Leptospira santarosai ) Leptospira noguchii , Listeria monocytogenes , Mycobacterium leprae , Mycobacterium tuberculosis , Mycobacterium ulceran s ), Mycoplasma pneumoniae , Neisseria gonorrhoeae , Neisseria meningitides , Nocardia asteroids , Rickettsia rickettsia ( Rickettsia rickettsia ), Salmonella enteritidis ( Salmonella enteritidis ), Salmonella typhi ( Salmonella typhi ), Salmonella paratyphi ( Salmonella paratyphi ), Salmonella typhimurium ( Salmonella typhimurium ), Shigella flexnerii , Shigella flexnerii ) Liae ( Shigella dysenteriae ), Staphylococcus sapropyticus , Streptococcus pneumoniae , Streptococcus pneumoniae , Streptococcus pyogenes , Gardnerella vaginalis ( Gardnerella vaginalis ), Streptococcus viridans , Treponema pallidum , Ureaplasma urealyticum , Vibrio cholera , Vibrio parahaemolyticum parahaemolyticus ), Yersinia pestis , Yersinia enterocolitica , Yersinia pseudotuberculosis ), Actinobacter baumannii ( Actinobacter baumanii ), Pseudomonas aeruginosas aeruginosa ), and mixtures thereof. In one embodiment the targeted bacterium of interest is Escherichia coli, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter claw Acae, and Enterobacter aerogenes, and mixtures thereof.

In certain embodiments, the targeted bacterial cell is, without limitation, Anaerotruncus , Acetanaerobacterium , Acetitomaculum , Acetivibrio, Anaerocco . Cus ( Anaerococcus ), Anaerofilum ( Anaerofilum ), Anaerosinus ( Anaerosinus ), Anaerostipes ( Anaerostipes ), Anaerovorax ( Anaerovorax ), Butyrivibrio ), Clostridium ( Clostridium ), Capracoccus , Dehalobacter , Dialister , Dorea , Enterococcus , Ethanoligenens , Faecalibacterium , Fusobacterium , Gracilibacter , Guggenheimella , Hespellia , Lachnobacterium , Lachnospira , Lactobacillus , Leuconostoc , Megamonas , Moryella , Mitsuokella , Oribacterium , Oxobacter, Papillibacter , Proprionispira ( Proprionispira ), Pseudobutyrivibrio , Pseudoramibacter , Roseburia , Ruminococcus , Sarcina , Seinonell a ), Shuttleworthia , Sporobacter , Sporobacterium , Streptococcus , Subdoligranulum , Syntrophococcus, Thermobacillus ( Thermobacillus ), Turibacter , Weisella , Clostridium , Bacteroides , Ruminococcus , Faecalibacterium , Treponema ), Phascolarctobacterium , Megasphaera , Faecalibacterium , Bifidobacterium , Lactobacillus , Sutterella , and/or Pre This is Prevotella .

In another embodiment, the targeted bacterial cell is, without limitation, Achromobacter xylosoxidans , Acidaminococcus fermentans , Acidaminococcus intestini . , Acidaminococcus sp., Acinetobacter baumannii , Acinetobacter junii , Acinetobacter lwoffii , Actinobacillus capsulatus ), Actinomyces naeslundi ( Actinomyces naeslundii ), Actinomyces sister ( Actinomyces neuii ), Actinomyces odontolyticus ( Actinomyces odontolyticus ), Actinomyces radingae ( Actinomyces radingae ), Adlercreutzia equolifaciens , Aeromicrobium massiliense , Aggregatibacter actinomycetemcomitans ), Akkermansia muciniphila , Akkermansia muciniphila Agarivorans marinus ( Aliagarivorans marinus ), Alistipes finegoldii , Alistipes indistinctus , Alistipes indistinctus , Alistipes inops , Alistipes onderdonkii ) , Alistipes putredinis , Alistipes senegalensis , Ali Stipes shahii ( Alistipes shahii ) , Alistipes timonensis , Alloscardovia omnicolens , Anaerobacter polyendosporus , Anaerobacterium hydro geniformans ( Anaerobaculum hydrogeniformans ), Anaerococcus hydrogenalis , Anaerococcus prevotii , Anaerococcus senegalensis , Anaerococcus senegalensis , Anaerococcus senegalensis Corihominis ( Anaerofustis stercorihominis ) , Anaerostipes caccae , Anaerostipes hadrus , Anaerotruncus colihominis , Anaerotruncus colihominis , Anaerostipes caccae ) Neuriticus ( Aneurinibacillus aneurinilyticus ), Bacillus licheniformis , Bacillus massilioanorexius , Bacillus massilioanorexius , Bacillus massiliosenegalensis ), Bacillus simplex simplex T ( Bacillus smithii ), Bacillus subtilis ( Bacillus subtilis ), Bacillus thuringiensis ( Bacillus thuringiensis ), Bacillus timonensis ( Bacillus timonensis ), Bacteriodes xylanisolvens ( Bacteriodes xylanisolvens ), Bacteriodes xylanisolvens ) Ens ( Bacteroides acidifaciens ), Bacteroides cacae ( B acteroides caccae ), Bacteroides capillosus , Bacteroides cellulosilyticus , Bacteroides clarus , Bacteroides coprocola Bacteroides coprophilus , Bacteroides dorei , Bacteroides eggerthii , Bacteroides faecis , Bacteroides finegoldii Bacteroides fluxus , Bacteroides fragilis , Bacteroides gallinarum , Bacteroides intestinalis , Bacteroides nordii , Bacteroides oleiciplenus , Bacteroides ovatus , Bacteroides pectinophilus , Bacteroides plebeius , Bacteroides sala Bacteroides salanitronis , Bacteroides salyersiae , Bacteroides sp., Bacteroides stercoris , Bacteroides thetaiotaomicron ( Bacteroides thetaiotaomicron ) , Bacteroides uniformis , Bacteroides uniformis Bacteroides vulgatus , Bacteroides xylanisolvens , Bacteroidespectinophilus ATCC, Barnesiella intestinihominis , Bavariicoccus sailor seileri ), Bifidobacterium adolescentis , Bifidobacterium angulatum , Bifidobacterium animalis , Bifidobacterium bifidum , Bifidobacterium Bifidobacterium breve , Bifidobacterium catenulatum , Bifidobacterium dentium , Bifidobacterium gallicum , Bifidobacterium longum longum ), Bifidobacterium pseudocatenulatum , Bifidobacterium stercoris , Bilophila wadsworthia, Blautia faecis , Blau Thia Hansenii ( Blautia hansenii ), Blautia hydrogenotropica ( Blautia hydrogenotrophica ), Blautia luti ( Blautia luti ), Blautia obeum , Blautia producta ( Blautia producta ), Blautia wexlerae , Brachymonas chir onomi ), Brevibacterium senegalense , Bryantella formatexigens , butyrate-producing bacteria, Butyricicoccus pullicaecorum , Butyricoccus virosa virosa, Butyricimonas virosa , Butyrivibrio crossotus ( Butyrivibrio crossotus ), Butyrivibrio fibrisolvens ( Butyrivibrio fibrisolvens ), Caldicoprobacter faecalis , Campylobacter concisus , Campylobacter Jejuni ( Campylobacter concisus ) Campylobacter jejuni ), Campylobacter upsaliensis , Catenibacterium mitsuokai , Cedecea davisae , Cellulomonas massiliensis , Cellulomonas massiliensis ) Somerae ( Cetobacterium somerae ), Citrobacter braakii ), Citrobacter freundii , Citrobacter pasteurii , Citrobacter pasteurii , Citrobacter sp., Citrobacter Yoongae ( Citrobacter youngae ), Cloacibacillus evryensis ( Cloacibacillus evryensis ), Clostridiales bacteria, Clostridiales difficile ( Clostridioides difficile ), Clostridium asparagiforme , Clostridium asparagiforme ) Iridium bartlettii ( Clostridium bartlettii ), Clostridium boli Biensis ( Clostridium boliviensis ), Clostridium bolteae ( Clostridium bolteae ) , Clostridium hathewayi , Clostridium hiranonis ), Clostridium hylemonae , Clostridium hylemonae ) Stridium leptum ( Clostridium leptum ), Clostridium methylpentosum ), Clostridium nexile ( Clostridium nexile ) , Clostridium orbiscindens ), Clostridium ramosum ( Clostridium ramosum ), Clostridium ramosum Stridium scindens ( Clostridium scindens ), Clostridium ( Clostridium ) sp, Clostridium ( Clostridium ) sp., Clostridium spiroforme ( Clostridium spiroforme ), Clostridium sporogenes ), Clost Clostridium symbiosum , Collinsella aerofaciens , Collinsella intestinalis , Collinsella stercoris , Collinsella tanakaei , copro Bacillus cateniformis ( Coprobacillus cateniformis ), Coprobacter fastidiosus ( Coprobacter fastidiosus ), Coprococcus catus ( Coprococcus catus ), Coprococcus cometh ( Coprococcus comes ), Coprococcus eutactus ( Coprococcus eutactus ) ), Corynebacterium ammoniagene s ), Corynebacterium amycolatum , Corynebacterium pseudodiphtheriticum , Cutibacterium acnes , Dermabacter hominis , desulphitobacter Lium hafniense ( Desulfitobacterium hafniense ), Desulfovibrio fairfieldensis , Desulfovibrio piger , Dialister succinatiphilus , Dielma fastidiosa ( Dielma fastidiosa ) , Dorea formicigenerans ( Dorea formicigenerans ), Dorea longicatena ( Dorea longicatena ), Dysgonomonas capnocytophagoides ( Dysgonomonas capnocytophagoides ), Dysgonomonas gadei ( Dysgonomonas gadei ), Dysgonomonas gadena mossii ), Edwardsiella tarda , Eggerthella lenta , Eisenbergiella tayi , Enorma massiliensis , Enterobacter aerogenes Enterobacter aerogenes , Enterobacter asburiae , Enterobacter cancerogenus , Enterobacter cloacae , Enterobacter massiliensis , Enterobacter massiliensis , Enterobacter cancerogenus ) ( Enterobacter casseliflavus ), Enterococcus durans ( Enterobacter durans ), Enterococcus faecalis , Enterococcus faecium , Enterococcus flavescens , Enterococcus gallinarum, Enterococcus gallinarum Enterococcus sp., Enterovibrio nigricans , Erysipelatoclostridium ramosum , Escherichia coli , Escherichia sp., Eubacterium sp. Biforme ( Eubacterium biforme ), Eubacterium dolichum ( Eubacterium dolichum ), Eubacterium hallii ( Eubacterium hallii ), Eubacterium limosum ( Eubacterium limosum ), Eubacterium ramulus ( Eubacterium ramulus ), Eubacterium Rectale ( Eubacterium rectale ) , Eubacterium siraeum , Eubacterium ventriosum , Exiguobacterium marinum , Exiguobacterium undae , Faecalibacterium ( Faecalibacterium ) cf, Faecalibacterium prausnitzii ), Faecalitalea cylindroides ), Ferrimonas balearica ( Ferrimonas balearica ), Pinegoldia magna ( Finegoldia ) magna ), Flavobacterium daejeonense ), Flavonifractor Plauti ( F ) lavonifractor plautii ), Fusicatenibacter saccharivorans , Fusobacterium gonidiaformans ), Fusobacterium fortiferum ( Fusobacterium mortiferum ), Fusobacterium necroforman necrophorum ), Fusobacterium nucleatum ( Fusobacterium nucleatum ), Fusobacterium periodonticum ( Fusobacterium periodonticum ), Fusobacterium ( Fusobacterium ) sp., Fusobacterium Ulcerans ( Fusobacterium ulcerans ), Fusobacterium Therium barium ( Fusobacterium varium ), Gallibacterium anatis , Gemmiger formicilis , Gordonibacter pamelaeae , Hafnia alvei , Helicobacter Helicobacter bilis , Helicobacter bills , Helicobacter canadensis , Helicobacter canis, Helicobacter canis , Helicobacter cinaedi , Helicobacter macacae , Helicobacter macacae Metensis ( Helicobacter pametensis ), Helicobacter pullorum ( Helicobacter pullorum ), Helicobacter pylori ( Helicobacter pylori ), Helicobacter rodentium ( Helicobacter rodentium ), Helicobacter winghamensis ( ) iliense ), Holdemanella biformis , Holdemania fdiformis , Holdemania filiformis, Holdemania massiliensis , Holdermania filiformis ( Holdemania filiformis ), Hungatella hathewayi , Intestinibacter bartlettii ), Intestinimonas butyriciproducens ), Klebsiella oxytoca oxytoca ( Klebsiella ) Klebsiella pneumoniae , Kurthia massiliensis , Lachnospira pectinoschiza , Lactobacillus acidophilus , Lactobacillus amylolyticus amylolyticus ), Lactobacillus animalis ( Lactobacillus animalis ), Lactobacillus antri ( Lactobacillus antri ), Lactobacillus brevis ( Lactobacillus brevis ), Lactobacillus buchneri ( Lactobacillus buchneri ), Lactobacillus casei ), Lactobacillus casei ( Lactobacillus casei ), Lactobacillus casei ( Lactobacillus curvatus ), Lactobacillus delbruecki ( Lactobacillus delbrueckii ), Lactobacillus fermentum ( Lactobacillus fermentum ), Lactobacillus gasseri ( Lactobacillus gasseri ), Lactobacillus helveticus ( Lactobacillus ) Lactobacillus hilgardii , Lactobacillus iners , Lactobacillus intestinalis , Lactobacillus johnsonii , Lactobacillus murinus , Lactobacillus murinus paracasei ), Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus reuteri ( Lactobacillus reuteri ), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus ruminis ( Lactobacillus sakei ), Lactobacillus sakei ), Lactobacillus ruminis ) Bacillus salivarius ( Lactobacillus salivarius ), Lactobacillus ultunensis ( Lactobacillus ultunensis ), Lactobacillus vaginalis ( Lactobacillus vaginalis ), Lactobacillus plantarum ( Lactobacillus plantarum ) subsp., leuconostoc mesenteroides ) Leuconostoc pseudomesenteroides , Listeria grayi , Listeria innocua , Mannheimia granulomatis , Marvinbryantia formatexigens ( Marvinbryantia) ), Megamonas funiformis ( Megamonas funiformis ), Megamonas hypermegale ( Megamonas hypermegale ), Methanobrevibacter smithii ), Methanobrevibacter smithii ethanobrevibacter smithii ) Fl, Micrococcus luteus , Microvirgula aerodenitrificans, Mitsuokella jalaludinii , Mitsuokella multacida Tess bacterium ( Mollicutes bacterium ), Murimonas intestini , Neisseria macacae , Nitriliruptor alkaliphilus , Oceanobacillus massiliensis , Odoribacter laneus ( Odoribacter laneus ), Odoribacter splanchnicus ( Odoribacter splanchnicus ), Ornithobacterium rhinotracheale ( Ornithobacterium rhinotracheale ), Oxalobacter formigenes ( Oxalobacter formigenes ), Paenibacillus ( Paenibacillus ) barengoltzii ), Paenibacillus chitinolyticus ), Paenibacillus lautus ( Paenibacillus lautus ), Paenibacillus motobuensis ( Paenibacillus motobuensis ), Paenibacillus senegalensis ), Paenibacillus senegalensis ( Paenibacillus senegalensis ) Sarcina quisquiliarum ( Paenisporosarcina quisquiliarum ) , Parabacteroides distasonis , Parabacteroides goldsteinii , Parabacteroides goldsteinii , Parabacteroides gordonii , onion La Bacteroides johnsonii ( Parabacteroides johnsonii ), Parabacteroides merdae ( Parabacteroides merdae ) , Paraprevotella xylaniphila ( Paraprevotella xylaniphila ), Parasutterella excrementihominis ( Parasutterella excrementihominis ), Parvimonas micra , Pediococcus acidilactici , Peptoclostridium difficile , Peptoniphilus harei , Peptoniphilus obesi , Peptoniphilus Senegalensis ( Peptoniphilus senegalensis ), Peptoniphilus timonensis ( Peptoniphilus timonensis ), Pascolactobacterium succinatutens ( Phascolarctobacterium succinatutens ), Porphyromonas asaccharolytica , Porphyromonas asaccharolytica ( Porphyromensis ) Porphyromonas uenonis , Prevotella baroniae , Prevotella bivia , Prevotella copri, Prevotella dentalis , Prevotella micans , Prevotella multisaccharivorax , Prevotella oralis, Prevotella salivae , Prevotella stercorea stercorea ), Prevotella veroralis ( Prevotella veroralis ), Propionibacterium Acne Propionibacterium acnes , Propionibacterium avidum , Propionibacterium freudenreichii , Propionimicrobium lymphophilum , Proteus mirabilis, Proteus mirabilis , Proteus penneri ( Proteuspenneri ) ATCC , Providencia alcalifaciens , Providencia rettgeri , Providencia rustigianii , Providencia stuartii ) , Pseudomonas fructor capillosus ( Pseudoflavonifractor capillosus ) , Pseudomonas aeruginosa ( Pseudomonas aeruginosa ), Pseudomonas luteola ( Pseudomonas luteola ), Ralstonia picketti ( Ralstonia leinheimera ), Ralstonia pickettii , Ralstonia pickettii ) , Rheinheimera texasensis , Riemerella columbina , Romboutsia lituseburensis , Roseburia faecis , Intestinal Roseburia faecis ) Lees ( Roseburia intestinalis ), Roseburia inulinivorans , Ruminococcus bicirculans , Ruminococcus bromii , Ruminococcus callidus , Ruminococcus bromii Luminococcus campanelensis ( R uminococcus champanellensis , Ruminococcus faecis , Ruminococcus gnavus , Ruminococcus lactaris , Ruminococcus obeum, Ruminococcus obeum sp, Ruminococcus sp., Ruminococcus torques , Sarcina ventriculi , Sellimonas intestinalis , Senegalimassilia anaerobia ), Shigella sonnei , Slackia piriformis , Staphylococcus epidermidis , Staphylococcus lentus , Staphylococcus Nepal Rensis ( Staphylococcus nepalensis ), Staphylococcus pseudintermedius ( Staphylococcus pseudintermedius ) , Staphylococcus xylosus , Stenotrophomonas maltophilia ), Streptococcus agalactia E ( Streptococcus agalactiae ), Streptococcus anginosus , Streptococcus australis , Streptococcus caballi , Streptococcus caballi , Streptococcus caballi ) Streptococcus didelphis , Streptococcus didelphis Leptococcus equinus ( Streptococcus equinus ), Streptococcus gordonii , Streptococcus henryi , Streptococcus henryi , Streptococcus infantaris infantaris , Streptococcus hyovaginalis ), Streptococcus infantis, Streptococcus lutetiensis , Streptococcus merionis, Streptococcus merionis , Streptococcus mitis , Streptococcus mitis Streptococcus mutans ), Streptococcus oralis , Streptococcus ovis , Streptococcus parasanguinis , Streptococcus plurextorum Streptococcus plurextorum ( Streptococcus porci ), Streptococcus pyogenes , Streptococcus salivarius , Streptococcus salivarius , Streptococcus sobrinus ), Streptococcus thermophilus , Streptococcus thermophilus Cus thoraltensis ( Streptococcus thoraltensis ), Streptomyces albus ), Subdoligranulum variabile , Succinatimonas hippei , Sutterella parvirubra ( Sutterella parvirubra ) , Sutterella wadsworthensis , Terrisporobacter glycolicus , Terrisporobacter mayombei , Thalassobacillus devorans , senegalenella sis ( Timonella senegalensis ), Turicibacter sanguinis , sp unknown, sp. unknown, Varibaculum cambriense , Veillonella atypica , Veillonella dispar dispar ), Veillonella parvula , Vibrio cincinnatiensis , Virgibacillus salexigens ) and Weissella paramesenteroides ).

In another embodiment, the targeted bacterial cells are those commonly present in the skin microbiota and include, but are not limited to, Acetobacter farinalis , Acetobacter malorum , Acetobacter orleanensis . , Acetobacter sicerae, Achromobacter anxifer , Achromobacter denitrificans , Achromobacter marplatensis, Achromobacter marplatensis , Achromobacter spanius ( Achromobacter spanius ), Achromobacter xylosoxidans ( Achromobacter xylosoxidans ) subsp. xylosoxidans , acidovorax konjaci , acidovorax radicis , Acinetobacter johnsonii , Actinomadura citrea , Actinomadura citrea Nomadura coerulea ( Actinomadura coerulea ), Actinomadura fibrosa ( Actinomadura fibrosa ), Actinomadura fulvescens , Actinomadura jiaoheensis , Actinomadura jiaoheensis , Actinomadura lutheofluores Sense ( Actinomadura luteofluorescens ), Actinomadura mexicana ( Actinomadura mexicana ), Actinomadura nitritigenes ( Actinomadura nitritigenes ) , Actinomadura verrucosospora , Actinomadura verrucosospora , y Actinomadura verrucosospora Actinomyces odontolyticus ( Actinomyces odontolyticus ), Actinomycetospora atypica ( Actinomycetospora atypica ), Actinomycetospora corticicola ( Actinomycetospora corticicola ), Actinomycetospora rhizophila ( Actinomycetospora ) rhizophila ), Actinomycetospora rishiriensis , Aeromonas australiensis , Aeromonas bestiarum , Aeromonas bivalvium Aeromonas bivalvrei ( Aeromonas encheleia ), Aeromonas eucrenophila ophila ), Aeromonas hydrophila ( Aeromonas hydrophila ) subsp. Hydrophila ( hydrophila ), Aeromonas piscicola , Aeromonas popoffii , Aeromonas rivuli , Aeromonas salmonicida subsp. Pectinolytica ( pectinolytica ), Aeromonas salmonicida ( Aeromonas salmonicida ) subsp. smithia , Amaricoccus kaplicensis , Amaricoccus veronensis, Aminobacter aganoensis, Aminobacter ciceronei , amino Bacteria lissarensis ( Aminobacter lissarensis ), Aminobacter niigataensis ( Aminobacter niigataensis ), Ancilobacter polymorphus ( Ancylobacter polymorphus ), Anoxybacillus flavithermus ( Anoxybacillus flavithermus ) subsp. yunnanensis , Aquamicrobium aerolatum , Archangium gephyra, Archangium gephyra , Archangium minus , Ar Archangium violaceum , Arthrobacter viscosus , Bacillus anthracis , Bacillus australimaris , Bacillus drentensis , Bacillus drentensis ( Bacillus mycoides ), Bacillus pseudomycoides ), Bacillus pumilus ( Bacillus pumilus ), Bacillus safensis ( Bacillus safensis ), Bacillus vallismortis ( Bacillus vallismortis ), Bosea thiooxidans ( Bosea thiooxidans ) , Bradyrhizobium huanghuaihaiense ( Bradyrhizobium huanghuaihaiense ), Bradyrhizobium japonicum ( Bradyrhizobium japonicum ), Brevundimonas aurantiaca ( Brevundimonas aurantiaca ), Brevundimonas intermedia ( Brevundimonas intermedia ), Brevundimonas intermedia ) Burkholderia aspalathi, Burkholderia choica , Burkholderia cordobensis , Burkholderia diffusa , Burkholderia insulsa ), Burkholderia rhynchosiae ( Burkholderia rhynchosiae ), Burkholderia terrestris ( Burkholderia terrestris ), Burkholderia udeis ( Burkholderia udeis ), Buttiauxella gaviniae ( Buttiauxella gaviniae ), Caenimonas terrae ( Caenimonas terrae ), Capnocytopaga gingivalis ( Capnocytophaga gingivalis ), Chitinophaga dinghuensis , Chryseobacterium gleum , Chryseobacterium greenlandense , Chryseobacterium jejuense, Chryseobacterium jejuense Chryseobacterium piscium, Chryseobacterium sediminis , Chryseobacterium tructae , Chryseobacterium ureilyticum, Chryseobacterium ureilyticum, Chryseobacterium ureilyticum Namens ( Chryseobacterium vietnamense ), Corynebacterium accolens ( Corynebacterium accolens ), Corynebacterium afermentans ( Corynebacterium afermentans ) subsp. Lipophilum ( lipophilum ), Corynebacterium minutissimum ( Corynebacterium minutissimum ), Corynebacterium sundsvallens ( Corynebacterium sundsvallens ), Cupriavidus metallidurans ( Cupriavidus metallidurans ), Cupriavidus nantongensis ( Cupriavidus nantongensis ), Cupriavidus necator ( Cupriavidus necator ), Cupriavidus pampae ( Cupriavidus pampae ), Cupriavidus yeoncheonensis ( Cupriavidus yeoncheonensis ), Curtobacterium flaccumiens ( Curtobacterium flaccumiens ) ), Devosia epidermidihirudinis ( Devosia epidermidihirudinis ), Devosia riboflavina ( Devosia riboflavina ) , Devosia riboflavina , Diaphorobacter oryzae ( Diaphorobacter oryzae ), Dietzia Caliphila ( Dietzia psychralcaliphila ), Ensifer adhaerens ( Ensifer adhaerens ), Ensifer americanus ( Ensifer americanus ), Enterococcus malodoratus ( Enterococcus malodoratus ), Enterococcus pseudoavium ( Enterococcus pseudoavium ) Enterococcus viikkiensis , Enterococcus xiangfangensis , Erwinia rhapontici , Falsilhodobacter halotolerans, Flavo Flavobacterium araucananum ), Flavobacterium frigidimaris ( Flavobacterium frig ) idimaris ), Gluconobacter frateurii , Gluconobacter thailandicus , Gordonia alkanivorans , Halomonas aquamarina , Halomonas aquamarina, Halomonas aquamarina ( Halomonas axialensis ), Halomonas meridiana ( Halomonas meridiana ), Halomonas olivaria ( Halomonas olivaria ), Halomonas songnenensis ), Halomonas variabilis ( Halomonas variabilis ), Herbaspiryllum ) ( Herbaspirillum chlorophenolicum ), Herbaspirillum frisingense ( Herbaspirillum frisingense ), Herbaspirillum hiltneri ( Herbaspirillum hiltneri ), Herbaspirillum huttiense ( Herbaspirillum huttiense ) subsp. Putei , Herbaspirillum lusitanum , Herminiimonas fonticola , Hydrogenophaga intermedia , Hydrogenophaga pseudoflava , Kleb Shiella oxytoca ( Klebsiella oxytoca ), Kosakonia sacchari ( Kosakonia sacchari ), Lactobacillus delbruecki ( Lactobacillus delbrueckii ) subsp. Bulgaricus ( bulgaricus ), Lactobacillus mode stisalitol lerans ( Lactobacillus modestisalitolerans ), Lactobacillus plantarum ( Lactobacillus plantarum ) subsp. Argentoratensis ( argentoratensis ), Lactobacillus xiangfangensis ( Lactobacillus xiangfangensis ), Lechevalieria roselyniae ( Lechevalieria roselyniae ), Lentzea alvida ( Lentzea albida ), Lentzea californiensis , Lentzea californiensis ( Lentzea californiensis ) Leuconostoc carnosum ( Leuconostoc carnosum ), Leuconostoc citreum ( Leuconostoc citreum ), Leuconostoc gelidum ( Leuconostoc gelidum ) subsp. Gasicomitatum ( gasicomitatum ), Leuconostoc mesenteroides ( Leuconostoc mesenteroides ) subsp. Suionicum ( suionicum ), Luteimonas aestuarii , Lysobacter antibioticus , Lysobacter koreensis , Lysobacter oryzae , Magneto Spirillum moscoviens ( Magnetospirillum moscoviense ), Marinomonas alcarazii , Marinomonas primoryensis , Massilia aurea , Massilia jejuensis , Massilia jejuensis ) Ongiensis (Massilia kyonggiensis), Massilia timonae ( Massilia timonae ), Mesorhizobium acaciae , Mesorhizobium qingshengii ), Mesorhizobium shonense ( Mesorhizobium ), Mesorhizobium ) Lium haplocladi ( Methylobacterium haplocladii ) , Methylobacterium platani ( Methylobacterium platani ), Methylobacterium pseudosasicola ( Methylobacterium pseudosasicola ), Methylobacterium zatmanii ), Microbacterium oxydane ( Microbacterium oxydan ), Micromonospora chaiyapumensis ), Micromonospora chalcea , Macromonospora citrea ), Micromonospora coxensis ) , Micromonospora echinopusca ( Micromonos pora echinofusca ), Micromonospora halophytica , Micromonospora kangleipakensis , Micromonospora maritima , Micromonospora nigra , Micromonospora purpureochromogene ( Micromonospora purpureochromogene ), Micromonospora rhizosphaerae ( Micromonospora rhizosphaerae ), Micromonospora saelicesensis ( Micromonospora saelicesensis ), Microvirga subterranea ( Microvirga subterranea ) , Microvirga zambiensis ( Microvirga zambiensis ), Mycobacterium alvei ( Mycobacterium alvei ), Mycobacterium avium ( Mycobacterium avium ) subsp. Silvaticum ( silvaticum ), Mycobacterium colombiense ( Mycobacterium colombiense ), Mycobacterium conceptionense ( Mycobacterium conceptionense ), Mycobacterium conceptionense ( Mycobacterium conceptionense ), Mycobacterium parcinogen ( Mycobacterium farcinogenes ), Mycobacterium fortuitum ( Mycobacterium fortuitum ) subsp. Fortuitum ( fortuitum ), Mycobacterium goodii ( Mycobacterium goodii ), Mycobacterium insubricum ( Mycobacterium insubricum ), Mycobacterium llatzerense ( Mycobacterium llatzerense ), Mycobacterium neoaurum ( Mycobacterium ) neoaurum ), Mycobacterium neworleansense ( Mycobacterium neworleansense ), Mycobacterium obuense ), Mycobacterium peregrinum ( Mycobacterium peregrinum ), Mycobacterium saopaulense ( Mycobacterium saopaulense ) , Mycobacterium septicum ( Mycobacterium septicum ), Mycobacterium setense ( Mycobacterium setense ), Mycobacterium smegmatis ), Neisseria subflava ( Neisseria subflava ), Nocardia Liangen Nocardia lijiangensis , Nocardia thailandica , Novosphingobium barchaimii , Novosphingobium lindaniclasticum , Novosphingobium lindaniclasticum , Novosphingobium lindaniclasticum , Novosphingobium mathurense ( Novosphingobium mathurense ) , Ochrobactrum pseudogrignonense ), Oxalicibacterium solurbis ( Oxalicibacterium solurbis ), Para Burkholderia glathei ( Paraburkholderia glathei ), Para Burkholderia glathei ) Leah Humi ( Paraburkholderia humi ), Paraburkholderia phenazinium ( Paraburkholderia phenazinium ), Paraburkholderia phytofirmans ( Paraburkholderia phytofirmans ), Paraburkholderia sordidicola ( Paraburkholderia sordidicola ), Paraburkholderia terricola ( Paraburkholderia terricola ), Para Burkholderia xenoborans xenovorans ), Paracoccus laeviglucosivorans , Patulibacter ginsengiterrae , Polymorphospora rubra , Porphyrobacter colymbi Prevotella jejuni ( Prevotella jejuni ), Prevotella melaninogenica ( Prevotella melaninogenica ), Propionibacterium acnes ( Propionibacterium acnes ) subsp. Elongatum ( elongatum ), Proteus vulgaris ( Proteus vulgaris ), Providencia rustigianii ( Providencia rustigianii ), Pseudoalteromonas agarivorans ( Pseudoalteromonas agarivorans ), Pseudoalteromonas atlantica ( Pseudoalteromonas atlantica ) Pseudoalteromonas paragorgicola , Pseudomonas asplenii , Pseudomonas asuensis, Pseudomonas asuensis , Pseudomonas benzenivorans sicola, Pseudomonas benzenivorans cannabinas , Pseudomonas benzenivorans cannabina Pseudomonas congelans ( Pseudomonas congelans ) , Pseudomonas costantinii ( Pseudomonas costantinii ), Pseudomonas ficuserectae ( Pseudomonas ficuserectae ), Pseudomonas Frederiks bergensis ( Pseudomonas frederiks gramensis ) Pseudomonas frederiksbergensis ( Pseudomonas frederiksbergen ) Pseudomonas jessenii ), Pseudomonas koreensis , Pseudomonas koreensis , Pseudomonas koreensis , Pseudomonas kunmingensis ) ( Pseudomonas panacis ), Pseudomonas plecoglosticida ( Pseudomonas plecoglosticida ), Pseudomonas poae ( Pseudomon as poae ), Pseudomonas pseudoalcaligenes , Pseudomonas putida , Pseudomonas reinekei ( Pseudomonas reinekei ), Pseudomonas reinekei ) Pseudomonas seleniipraecipitans ) , Pseudomonas umsongensis , Pseudomonas zhaodongensis , Pseudomonas zhaodongensis , Pseudomonas alaniniphila ia ) Autotropica ( Pseudonocardia autotrophica ), Pseudonocardia kongjuensis , Pseudonocardia yunnanensis ), Pseudonocardia yunnanensis ) ), Pseudo xanthomonas indica ( Pseudoxanthomonas indica ), Pseudoxanthomonas kaohsiungensis ), Psychrobacter aquaticus , Psychrobacter arcticus ), Psychrobacter celer , Psychrobacter marincola , Psychrobacter nivimari , Psychrobacter okhotskensis, Psychrobacter okhotskensis Psychrobacter okhotskensis , Psychrobacter piscatorii , Psychrobacter pulmonis , Ramlibacter ginsenosidimutans , Rheinheimera japonica Rheinheimera Rheinheimera muenzenbergensis , Rheinheimera soli , Rheinheimera tangshanensis, Rheinheimera tangshanensis , Rheinheimera texasensis , Rheinheimera apiheasensis , Rhizobium alamii , Rhizobium azibense , Rhizobium binae, Rhizobium daejeonense , Rhizobium daejeonense , Rhizobium endophyticum endophyticum Rhizobium etli , Rhizobium fabae, Rhizobium freirei, Rhizobium gallicum , Rhizobium loessense , Rhizobium sophoriradicis ), Rhizobium taibaishanense , Rhizobium vallis, Rhizobium vignae, Rhizobium vignae , Rhizobium yanglingens ( Rhizobium yanglingense ) , Rhodococcus baikonurensis ), Rhodococcus enclensis , Rhodoferax Saidenbachensis , Rickettsia canadensis , Rickettsia heilongjiangensis , Rickettsia heilongjiangensis Horne ( Rickettsia honei ), Rickettsia raoultii ( Rickettsia raoultii ), Roseateles aquatilis ( Roseateles aquatilis ), Roseateles aquatilis ( Roseateles aquatilis ), Salmonella enterica ( Salmonella enterica ) subsp. Salamae ( salamae ), Serratia ficaria ( Serratia ficaria ), Serratia myotis ( Serratia myotis ), Serratia vespertilionis , Shewanella aestuarii , Shu Wanella decolorationis ( Shewanella decolorationis ), Sphingobium amiense , Sphingobium baderi , Sphingobium barthaii , Sphingobium chlorophenolicum ) , Sphingobium cupriresistens ( Sphingobium cupriresistens ) , Sphingobium czechense ), Sphingobium fuliginis , Sphingobium indicum ( Sphingobium indicum ), Sphingobium indicum ), Sphingobium japonicum, Sphingobium lactosutens , Sphingomonas dokdonensis , Sphingomonas pseudosanguinis, Sphingomonas pseudosanguinis Sphingopyxis chilensis , Sphingopyxis fribergensis , Sphingopyxis granuli , Sphingopyxis indica , Sphingopyxis witflariensis, Sphingopyxis witflariensis Staphylococcus agnetis ( Staphylococcus agnetis ), Staphylococcus aureus ( Staphylococcus ) aureus ) subsp. Aureus ( aureus ), Staphylococcus epidermidis , Staphylococcus epidermidis , Staphylococcus hominis ) subsp. Novobiosepticus ( novobiosepticus ), Staphylococcus nepalensis ), Staphylococcus sapropyticus ( Staphylococcus sapropyticus ) subsp. Bovis ( bovis ), Staphylococcus sciuri ( Staphylococcus sciuri ) subsp. Carnaticus ( carnaticus ), Streptomyces caeruleatus ), Streptomyces canarius ( Streptomyces canarius ), Streptomyces capoamus ( Streptomyces capoamus ), Streptomyces ciscaucasicus ciscaucasicus ), Streptomyces griseorubiginosus ), Streptomyces olivaceoviridis ), Streptomyces panaceoviridis ), Streptomyces panaciradicis pureus ) ), Streptomyces pseudovenezuelae , Streptomyces resistomycificus , Tianweitania sediminis , Tsukamurella paurometabola Lax guanciensis ( Variovorax guangxiensis ) , Vogesella alkaliphila ), Xanthomonas arboricola ( Xanthomonas arboricola ), Xanthomonas axonopodis ), Xanthomonas cassavae ( Xanthomonas cassavae ) Vitae ( Xanthomonas cucurbitae ), Xanthomonas cynarae , Xanthomonas euvesicatoria , Xanthomonas fragariae , Xanthomonas gardnoe eri ), Xanthomonas perforans , Xanthomonas pisi , Xanthomonas populi , Xanthomonas vasicola, Xenophilus aerolatus , Xenophilus aerolatus Yersinia nurmii , Abiotrophia defectiva , Acidocella aminolytica , Acinetobacter guangdongensis , Acinetobacter parvus, Acinetobacter parvus Acinetobacter radioresistens ( Acinetobacter radioresistens ), Acinetobacter soli ( Acinetobacter soli ), Acinetobacter variabilis ( Acinetobacter variabilis ), Actinomyces cardiffensis ( Actinomyces cardiffensis ), Actinomyces dentalis ( Actinomyces dentalis ), Actinomyces europaeus ( Actinomyces europaeus ), Actinomyces gerencseriae ( Actinomyces gerencseriae ), Actinomyces graevenitzii ( Actinomyces graevenitzii ), Actinomyces haliotis ( Actinomyces ) haliotis ), Actinomyces johnsonii ( Actinomyces johnsonii ), Actinomyces massiliensis ), Actinomyces meyeri ( Actinomyces meyeri ), Actinomyces meyeri ( Actinomyces meyeri ), Actino Myces naeslundi ( Actinomyces naeslundii ), Actinomyces sister ( Actinomyces neuii ) s ubsp. Anithratus ( anitratus ), Actinomyces odontolyticus ( Actinomyces odontolyticus ), Actinomyces oris ( Actinomyces oris ), Actinomyces turicensis ( Actinomyces turicensis ), Actinomycetospora cortisci Coke ( Actinomycetospora corticicola ), Actinotignum schaalii ( Actinotignum schaalii ), Aerococcus christensenii , Aerococcus urinae , Aeromicrobium flavum ( Aeromicrobium ) Microbium massiliense ( Aeromicrobium massiliense ), Aeromicrobium tamlense ( Aeromicrobium tamlense ), Aeromonas sharmana ( Aeromonas sharmana ), Aggregatibacter aphrophilus , Aggregatibacter segnis segnis ), Agrococcus baldri ( Agrococcus baldri ), Albibacter methylovorans ( Albibacter methylovorans ), Alcaligenes faecalis ( Alcaligenes faecalis ) subsp. Faecalis ( faecalis ), Algoriphagus ratkowskyi ( Algoriphagus ratkowskyi ) , Alkali Bacteria olivapovliticus ( Alkalibacterium olivapovliticus ), Alkali Bacteria Pelagium ( Alkalibacterium pelagium ), Alkali Bacterium Pelagium ( Alkalibacterium pelagium ) Prevotella rava ( Alloprevotella rava ), Alsobacter metallidurans ( Alsobacter metallidurans ) , Amaricoccus kaplicensis , Amaricoccus veronensis , Anaerococcus hydrogenalis ( Anaerococcus hydrogenalis ), Anaerococcus lactolyticus , Anaerococcus murdochii ) Rococcus vaginalis ( Anaerococcus vaginalis ), Aquabacterium citratiphilum , Aquabacterium olei , Aquabacterium olei , Aquabacterium olei , Aquabacterium parvum , aquine Cola tertiaricarbonis ( Aquincola tertiaricarbonis ), Arcobacter venerupis ( Arcobacter venerupis ), Arsenicicoccus bolidensis ( Arsenicicoccus bolidensis ), Arthrobacter russicus ) ( Asticcacaulis excentricus ), atophobia Atopobium deltae , Atopobium parvulum , Atopobium rimae , Atopobium vaginae , Aureimonas altamirensis , Aureimonas rubiginis , Azospira oryzae , Azospirillum oryzae , Bacillus circulans , Bacillus drentensis , Bacillus drentensis Pastidiosus ( Bacillus fastidiosus ), Bacillus lehensis ( Bacillus lehensis ), Bacillus oceanisediminis ( Bacillus oceanisediminis ), Bacillus rhizosphaerae ( Bacillus rhizosphaerae ), Bacteriovorax stolpii ( Bacteriovorax stolpii ), Bacteriovorax stolpii Bacteroides coagulans , Bacteroides dorei , Bacteroides fragilis , Bacteroides ovatus , Bacteroides stercoris stercoris Bacteroides uniformis , Bacteroides vulgatus , Bdellovibrio bacteriovorus , Bdellovibrio exovorus , Belnapia moabensis ), Belnapia soli , Blautia hansenii , Blautia obeum , Blautia wexlerae , Bosea lathyri , Brachybacterium fresconis , Brachybacterium muris , Brevibacterium ammoniilyticum ( Brevibacterium ammoniilyticum ), Brevibacterium casei ( Brevibacterium casei ), Brevibacterium epidermidis ( Brevibacterium epidermidis ), Brevibacterium iodinum ( Brevibacterium iodinum ), Brevibacter Leum luteolum ( Brevibacterium luteolum ), Brevibacterium paucivorans ( Brevibacterium paucivorans ), Brevibacterium pityocampae ( Brevibacterium pityocampae ), Brevibacterium sanguinis , Brevibacterium sanguinis ) Brevundimonas albigilva ), Brevundimonas diminuta ( Brevundimonas diminuta ), Brevundimonas vancanneytii ), Caenimonas terrae ( Caenimonas terrae ), Calidifontibacter indicus bacterium ( Calidifontibacter ) Campylobacter concisus , Campylobacter gracilis , Campylobacter hominis , Campylobacter rectus , Campylobacter showae , Campylobacter showae Philobacter ureolyticus ( Campylobacter ureolyticus ), Capnocytophaga gingivalis ( Capnocytoph aga gingivalis ), Capnocytophaga leadbetteri , Capnocytophaga ochracea , Capnocytophaga sputigena , Cardiobacterium hominis ( Cardiobacterium hominis ) Cardiobacterium valvarum , Carnobacterium divergens , Catonella morbi, Caulobacter henricii , Cavicella subterranea , Cellulomonas xylanilytica ( Cellulomonas xylanilytica ), Cellvibrio vulgaris ( Cellvibrio vulgaris ), Chitinimonas taiwanensis ( Chitinimonas taiwanensis ), Chryseobacterium arachidis ( Chryseobacterium arachidis ), Chryseobacterium Daejeon ( Chryseobacterium daecheongense ), Chryseobacterium formosense , Chryseobacterium formosense ), Chryseobacterium greenlandense ), Chryseobacterium indologenes ( Chryseobacterium formosense ) indologenes ), Chryseobacterium piscium , Chryseobacterium rigui , Chryseobacterium solani , Chryseobacterium taklimakanense , Chryseobacterium taklimakanense Bacterium Ureality Chryseobacterium ureilyticum , Chryseobacterium ureilyticum , Chryseobacterium zeae, Chryseobacterium zeae , Chryseobacterium aureum ( Chryseomicrobium aureum ), Cloacibacterium halio ), Cloacibacterium normanense ( Cloacibacterium normanense ) , Cloacibacterium normanense ( Cloacibacterium normanense ), Collinsella aerofaciens ), Comamonas denitrificans ( Comamonas denitrificans ), Comamonas denitrificans ) Gena ( Comamonas terrigena ), Corynebacterium accolens ( Corynebacterium accolens ), Corynebacterium afermentans ( Corynebacterium afermentans ) subsp. Lipophilum ( lipophilum ), Corynebacterium ammoniagenes ( Corynebacterium ammoniagenes ), Corynebacterium amycolatum ( Corynebacterium amycolatum ), Corynebacterium aurimucosum ( Corynebacterium aurimucosum ), Corynebacterium aurimuco Breath ( Corynebacterium aurimucosum ) , Corynebacterium coyleae , Corynebacterium durum , Corynebacterium freiburgense , Corynebacterium glaucum ), Corynebacterium glyciniphilum , Corynebacterium imitans , Corynebacterium jeikeium , Corynebacterium jeikeium, Corynebacterium jeikeium Corynebacterium kroppenstedtii , Corynebacterium lipophiloflavum , Corynebacterium massiliense , Corynebacterium mastitidis, Corynebacterium mastitidis Nebacterium matruchotii ( Corynebacterium matruchotii ), Corynebacterium minutissimum ( Corynebacterium minutissimum ), Corynebacterium mucifaciens , Corynebacterium mustelae ( Corynebacterium mustelae ), Bacterium mycetoides ( Corynebacterium mycetoides ), Corynebacterium pyruviciproduscens ( Corynebacterium pyruviciproducens ), Corynebacterium simulans ( Corynebacterium simulans ), Corynebacterium singulare ( Corynebacterium singulare ), Corynebacterium sputi ( Corynebacterium sputi ), Coryne Bacterium suicordis ( Corynebacterium suicordis ), Corynebacterium tuberculostearicum ( Corynebacterium tuberculostearicum ), Corynebacterium tuberculostearicum ( Corynebacterium tuberculostearicum ), Corynebacterium ureiselleriborans ( Corynebacterium ) ureicelerivorans ), Corynebacterium variabile , Couchioplanes caeruleus subsp. Caeruleus ( caeruleus ), Cupriavidus metallidurans ( Cupriavidus metallidurans ), Curtobacterium herbarum ( Curtobacterium herbarum ), Dechloromonas agitata ( Dechloromonas agitata ), Deinococcus actinoscleus ( Deinococcus ) actinosclerus ), Deinococcus antarcticus , Deinococcus caeni , Deinococcus ficus , Deinococcus geothermalis , Deinococcus geothermalis , Deinococcus geothermalis ) Rans ( Deinococcus radiodurans ), Deinococcus wulumuqiensis ( Deinococcus wulumuqiensis ), Deinococcus xinjiangensis , Dermabacter hominis ( Dermabacter hominis ), Dermabacter vaginalis ), Dermabacter vaginalis ( Dermabacter vaginalis ) Dermacoccus nishinomiyaensis , Desemzia incerta , Desertibacter roseus , Dialister invisus , Dialister micraerophilus Dialister micraerophilus Dialister propionicifaciens , Dietzia aurantiaca , Dietzia cercidiphylli , Dietzia timorensis , Dietzia timorensis sis ( Dietzia timorensis ), Dokdonella koreensis , Dokdonella koreensis ) Dokdonella koreensis , Dolosigranulum pigrum , Eikenella corrodens , Elizabethkingia miricola , Elstera litoralis , Empedo Bacter brevis ( Empedobacter brevis ), Enhydrobacter aerosaccus ( Enhydrobacter aerosaccus ), Enterobacter xiangfangensis , Enterococcus aquimarinus ( Enterococcus aquimarinus ) , Enterococcus faecali, Enterococcus faecali Enterococcus olivae, Erwinia rhapontici , Eubacterium eligens , Eubacterium infirmum, Eubacterium rectale , Eubacterium saphenum ( Eubacterium saphenum ), Eubacterium sulci ( Eubacterium sulci ), Exiguobacterium mexicanum , Facklamia tabacinasalis , Falsirhodobacter halotoleran halotolerans ), Finegoldia magna , Flavobacterium cutihirudinis , Flavobacterium lindanitolerans , Flavobacterium resistens ( Flavobacterium resistens ), Edmanniella capsulata ( Friedmanniella capsulata ), Fusobacteria Um nucleatum ( Fusobacterium nucleatum ) subsp. Polymorphum ( polymorphum ), Gemella haemolysans , Gemella morbillorum , Gemella palaticanis , Gemella sanguinis , Gemmobacter Aquaticus ( Gemmobacter aquaticus ), Gemmobacter caeni , Gordonia jinhuaensis , Gordonia kroppenstedtii ), Gordonia polyisoprenivorans ( Gordonia polyisoprenivorans ), Gordonia polyisoprenivorans ( Gordonia polyisoprenivorans ), Granulicatella adiacens ( Granulicatella adiacens ), Granulicatella elegans ), Haemophilus parainfluenzae , Haemophilus parainfluenzae ) Torum ( Haemophilus sputorum ), Haemophilus sulfidaeris ( Haemophilus sulfidaeris ), Herpetosiphon aurantiacus ( Herpetosiphon aurantiacus ), Hydrocarboniphaga effusa ( Hydrocarboniphaga effusa ), Idiomarina maris ( Idiomarina maris ) Janibacter anophelis , Janibacter hoylei , Janibacter indicus , Janibacter limosus , Janibacter melonis , Zeotgalicocus halophyll Ruth ( Jeotgalicoccus halophilus ), Jonquetella anthropi ( Jonquetella anthropi ), Kaistia geumhonensis , Kingella denitrificans , Kingella oralis , Klebsiella oxytoca , Knoellia aerolata ), Knoellia locipacati, Kocuria atrinae , Kocuria carniphila , Kocuria kristinae , Kocuria palustris , Cocuria turfanensis ( Kocuria turfanensis ), Lachnoanaerobaculum saburreum ( Lachnoanaerobaculum saburreum ), Lachnoanaerobaculum saburreum ( Lachnoanaerobaculum saburreum ), Lactobacillus crispatus ( Lactobacillus ) Bacillus inus ( Lactobacillus iners ), Lactococcus lactis ( Lactococcus lactis ) subsp. Lactis ( lactis ), Lactococcus lactis ( Lactococcus lactis ) subsp. Lactis ( lactis ), Lactococcus piscium ( Lactococcus piscium ), Lapillicoccus jejuensis , Lautropia mirabilis , Legionella beliardensis , Legionella beliardensis ) Callis ( Leptotrichia buccalis ), Leptotrichia goodfellowii ( Leptotrichia goodfellowii ), Leptotrichia hofstadii , Leptotrichia hofstadii , Leptotrichia hongkongensis ( Leptotrichia hongkongensis ), Leptotrichia shahi ( Leptotrichia shahi ), Leptotrichia shahi ( Leptotrichia shahi ) Leptotrichia trevisanii , Leptotrichia wadei , Luteimonas terricola , Lysinibacillus fusiformis , Lysobacter spongiicola , Lysobacter spongii Lysobacter xinjiangensis , Macrococcus caseolyticus , Marmoricola pocheonensis , Marmoricola scoriae , Massilia alkalitolerans , drinkable Ria alkali tolerans ( Massilia alkalitolerans ), Massilia aurea ( Massilia aurea ), Massilia plicata ( Massilia plicata ), Massilia timonae ( Massilia timonae ), Megamonas rupellensis ( Megamonas rupellensis ), Mayothermus Sylvanus ( Meiothermus silvanus) ), Methylobacterium dankookense , Methylobacterium goesingense , Methylobacterium goesingense, Methylobacterium isviliens ( Methylobacterium isbiliense ), Methylobacterium jeotgali , Methylobacterium oxalidis ), Methylobacterium platani , Methylobacterium pseudosasicola ) , Methyloversatilis universalis , Microbacterium foliorum , Microbacterium hydrothermale , Microbacterium hydrothermale, Microbacterium hydrothermale , Microbacterium lacticum ( Microbacterium lacticum ), Microbacterium lacticum ( Microbacterium lacticum ), Microbacterium laevaniformans ( Microbacterium laevaniformans ), Microbacterium paludicola ( Microbacterium paludicola ), Microbacterium petrolearium ( Microbacterium petrolearium ) , Microbacterium phyllosphaerae , Microbacterium resistens , Micrococcus antarcticus , Micrococcus cohnii , Micrococcus flavus flavus ), Micrococcus lylae ( Micrococcus lylae ) , Micrococcus terreus ), Microlunatus aurantiacus ( Microlunatus aurantiacus ), Micropruina glycogenica ( Micropruina glycogenica ), microvirga Microvirga aerilata , Microvirga aerilata, Microvirga subterranea , Microvirga vignae , Microvirga zambiensis ), Microvirgula aerodenitrificans, Mogibacterium timidum , Moraxella atlantae , Moraxella catarrhalis , Morganella mor Gani ( Morganella morganii ) subsp. Morganii ( morganii ), Morganella cyclotolerans ( Morganella psychrotolerans ), Murdochiella asaccharolytica ( Murdochiella asaccharolytica ), Mycobacterium asiaticum ( Mycobacterium asiaticum ), Mycobacterium chubuense ( Mycobacterium chubuense ) , Mycobacterium crocinum ( Mycobacterium crocinum ), Mycobacterium gadium ( Mycobacterium gadium ), Mycobacterium holsaticum ( Mycobacterium holsaticum ), Mycobacterium iranicum ( Mycobacterium iranicum ), Mycobacterium Lium longobardum ( Mycobacterium longobardum ), Mycobacterium neoaurum ( Mycobacterium neoaurum ), Mycobacterium neoaurum ( Mycobacterium neoaurum ), Mycobacterium obuense ( Mycobacterium obuense ), Negatibicocus succini Siborans (Negativicoccus succinicivorans ), Neisseria bacilliformis ( Neisseria bacilliformis ), Neisseria oralis ( Neisseria oralis ), Neisseria sicca ( Neisseria sicca ), Neisseria subflava ( Neisseria subflava ) Nesterenkonia lacusekhoensis ), Nesterenkonia rhizosphaerae , Nevskia persephonica , Nevskia ramosa ), Niabella yanteshanensis , yanshanensis Umoris ( Niveibacterium umoris ), Nocardia niwae ( Nocardia niwae ) , Nocardia thailandica , Nocardioides agariphilus , Nocardioides dilutus , Nocardioides ganghwensis, Nocardioides ganghwensis Nensis ( Nocardioides hwasunensis ) , Nocardioides nanhaiensis ( Nocardioides nanhaiensis ), Nocardioides sediminis ( Nocardioides sediminis ), Nosocomiicoccus ampullae , Nosocomiicoccus ampullae ) Noviherbaspirillum malthae ), Novosphingobium lindaniclasticum , Novosphingobium rosa , Ochrobactrum rhizosphaerae , Olsenellae ul Microbium murale ( Ornithinimicrobium murale ) , Ornithinimicrobium tianjinense , Oryzobacter terrae , Ottowia beijingensis , Faenalcaligenes suwanensis Paenalcaligenes suwonensis ), Paenibacillus agaridevorans ( Paenibacillus agaridevorans ) , Paenibacillus phoenicis , Paenibacillus xylanexedensium ( Paenibacillus xylanexedens bacterium ), Paludibacterium , Pantoea cypripedii ), Parabacteroides distasonis ( Parabacteroides distasonis ), Paraburkholderia andropogonis ( Paraburkholderia andropogonis ), Paracoccus alkaline Pylus ( Paracoccus alcaliphilus ), Paracoccus angustae ( Paracoccus angustae ), Para Coccus cauliflower ( Paracoccus kocurii ), Paracoccus laeviglucosivorans ( Paracoccus laeviglucosivorans ), Paracoccus sediminis ), Paracoccus sphaerophysae ( Paracoccus sphaerophysae ), Paracoccus Paracoccus yeei , Parvimonas micra , Parviterribacter multiflagellatus , Patulibacter ginsengiterrae , Pedobacter aquatilis ), Pedobacter ginsengisoli , Pedobacter xixiisoli , Peptococcus niger , Peptoniphilus coxii , Peptoniphilus gorbaki , Peptoniphilus harei ( Peptoniphilus harei ), Peptoniphilus koenoeneniae ( Peptoniphilus koenoeneniae ), Peptoniphilus lacrimalis ( Peptoniphilus lacrimalis ), Peptostreptococcus anaerobius ( Peptostreptoccus ), Peptostreptococcus stomatis , Pascolactobacterium faecium ( Phascolarctob acterium faecium ), Phenylobacterium haematophilum , Phenylobacterium kunshanense , Pluralibacter gergoviae , Polymorphobactermanifer , Polymorphobactermanifer Porphyromonas bennonis , Porphyromonas endodontalis , Porphyromonas gingivalis, Porphyromonas gingivalis , Porphyromonas gingiviv pasteri ), Porphyromonas pogonae , Porphyromonas somerae , Povalibacter ubarum , Prevotella aurantiaca , Prevotella aurantiaca ) Prevotella baroniae , Prevotella bivia, Prevotella buccae, Prevotella buccalis , Prevotella copri , Prevo Prevotella corporis , Prevotella denticola , Prevotella enoeca , Prevotella histicola , Prevotella intermedia , Prevotella jejuni , Prevotella jejuni ), Prevotella maculosa , Prevotella melaninogenica , Prevotella melaninogenica, Prevotella melaninogenica, Prevotella micans , Prevotella multiformis ( Prevotella multiformis ), Prevotella nanceiensis , Prevotella nigrescens , Prevotella oris , Prevotella oulorum , Prevotella pal lance ( Prevotella pallens ), Prevotella pleuritidis , Prevotella saccharolytica , Prevotella salivae , Prevotella shahii ) Tela timonensis ( Prevotella timonensis ), Prevotella veroralis ( Prevotella veroralis ), Propionibacterium acidifaciens ( Propionibacterium acidifaciens ), Propionibacterium acnes ( Propionibacterium acnes ) subsp. Acne ( acnes ), Propionibacterium acnes ( Propionibacterium acnes ) subsp. Acne ( acnes ), Propionibacterium acnes ( Propionibacterium acnes ) subsp. Elongatum , Propionibacterium granulosum , Propionimicrobium lymphophilum, Propionispira arcuata , Pseudochineococcus lucitanus ( Pseudokineococcus lusitanus ) , Pseudomonas aeruginosa , Pseudomonas chengduensis , Pseudomonas chengduensis ) Psychrobacter sanguinis , Ramlibacter ginsenosidimutans , Rheinheimera aquimaris , Rhizobium alvei , Rhizobium daejeon Rhizobium daejeon Rhizobium larrymoorei , Rhizobium rhizoryzae , Rhizobium soli , Rhizobium taibaishanense , Rhizobium vignae, Rhizobium vignae Rhodanobacter glycinis , Rhodobacter veldkampii , Rhodococcus enclensis , Rhodococcus fascians , Rhodococcus fascians, Rhodococcus fascians clicus ( Rhodovarius lipoc) yclicus ), Rivicola pingtungensis , Roseburia inulinivorans , Roseenbergiella nectarea , Roseomonas aerilata, Roseomonas aerilata ) Aquatica ( Roseomonas aquatica ), Roseomonas mucosa , Roseomonas rosea , Roseomonas vinacea , Rothia aeria , Rotia amara ( Rothia amarae ), Rotia dentocariosa ( Rothia dentocariosa ), Rotia endophytica ( Rothia endophytica ), Rothia mucilaginosa ( Rothia mucilaginosa ), Rothia nasimurium ) Mesophyllum ( Rubellimicrobium mesophilum ), Rubellimicrobium roseum ( Rubellimicrobium roseum ), Rubrobacter bracarensis , Rudaea cellulosilytica ), Ruminococcus gravus ( Ruminococcus ) , Runella zeae ( Runella zeae ), Saccharopolyspora rectivirgula ( Saccharopolyspora rectivirgula ), Salinicoccus qingdaonensis ), Scardovia wiggsiae ( Scardovia wiggsiae ), Sediminibacterium Ginseng Solay ( Sediminibacterium ginsengisoli ), Selenomonas artemidis ( Selenomonas artemidis ), Selenomonas infelix ( Selenomonas in felix ), Selenomonas noxia , Selenomonas sputigena , Shewanella aestuarii , Shuttleworthia satelles , Simonsiela radish Ellery ( Simonsiella muelleri ), Skermanella aerolata ( Skermanella aerolata ), Skermanella stibiiresistens ( Skermanella stibiiresistens ), Slackia exigua ( Slackdicia exigua ), Smaragdicoccus niigatensis niigatensis ), Steatia sanguinegens ( Sneathia sanguinegens ), Solirubrobacter soli , Spingobacterium caeni , Sphingobacterium caeni , Sphingobacterium daejeonenseium , Spingobacterium hota Nence ( Sphingobacterium hotanense ), Spingobacterium kyonggiense ( Sphingobacterium kyonggiense ), Spingobacterium multivorum (Sphingobacterium multivorum), Spingobacterium nematocida (Sphingobacterium nematocida), Spingobacterium spirit borum ( Sphingobacterium spiritivorum ), Sphingobium amiense , Sphingobium indicum , Sphingobium lactosutens , Sphingobium subterraneum , Sphingobium subterraneum , Sphingobium subterraneum Poetry ( Sphingomonas abaci ), Sphingomonas aestuarii ( Sphingomonas aestuarii ), Spingo Monas canadensis ( Sphingomonas canadensis ), Sphingomonas daechungensis , Sphingomonas dokdonensis , Sphingomonas dokdonensis , Sphingomonas echinoides , Sphingomonas echinoides , Sphingomonas echinoides ), Sphingomonas fonticola , Sphingomonas formosensis , Sphingomonas gei , Sphingomonas hankookensis, Sphingomonas hankookensis , Sphingomonas hankoensis . ), Sphingomonas koreensis ( Sphingomonas koreensis ), Sphingomonas kyeonggiensis , Sphingomonas laterariae ( Sphingomonas laterariae ), Sphingomonas mucosissima ), Sphingomonas mucosissima ) Sphingomonas oligophenolica , Sphingomonas pseudosanguinis , Sphingomonas sediminicola , Sphingomonas yantingensis , Sphingomonas yunnanensis Sphingomonas yunnanensis , Spinopyxis indica ( Sphingopyxis indica ), Spirosoma rigui ( Spirosoma rigui ), Sporacetigenium mesophilum ( Sporacetigenium mesophilum ), Sporocytophaga myxococcoides ( Sporocytophaga myxococcoides ), Staphylococcoides ) ( Staphy lococcus auricularis ), Staphylococcus epidermidis ), Staphylococcus epidermidis ), Staphylococcus epidermidis , Staphylococcus hominis subsp. Novobiosepticus ( novobiosepticus ), Staphylococcus lugdunensis ( Staphylococcus lugdunensis ), Staphylococcus pettenkoferi ( Staphylococcus pettenkoferi ), Stenotrophomonas koreensis , Stenotrophomonas koreensis ) Stenotrophomonas rhizophila , Stenotrophomonas rhizophila , Streptococcus agalactiae , Streptococcus agalactiae, Streptococcus canis, Streptocstatus canis , Streptococcus cristococcus Streptococcus gordonii ( Streptococcus gordonii ), Streptococcus infantis ( Streptococcus infantis ), Streptococcus intermedius ( Streptococcus intermedius ), Streptococcus mutans ( Streptococcus Streptoccus mutans ), Streptococcus Streptococcus mutans, Streptococcus mutans oligofermentans ), Streptococcus oralis , Streptococcus sanguinis , Streptomyces iconiensis , Streptomyces aquayanglinensis Streptomyces ) ( Tabrizicola aquatica ), Tahibacter caeni , Tannerella forsythia , Tepidicella xavieri , Tepidimonas fonticaldi , Terracoccus luteus Terracoccus luteus ), Tessaracoccus flavescens , Thermus thermophilus , Tianweitania sediminis , Tianweitania sediminis , Treponema amylobo Room ( Treponema amylovorum ), Treponema denticola ( Treponema denticola ), Treponema lecithinolyticum , Treponema medium ( Treponema medium ), Turicella otitidis , Turicella otitidis ) Sanguinis ( Turicibacter sanguinis ), Undibacterium oligocarboniphilum ( Undibacterium oligocarboniphilum ) , Undibacterium squillarum ( Undibacterium squillarum ), Vagococcus salmoninarum ( Vagococcus salmoninarum ), Varibaculum cambriens ( Varibaculum cambriense ) , Vibrio metschnikovii , Xanthobacter tagetidis , Xenophilus aerolatus , Xenophilus arseniciresistens , Imela lutea ( Yimella lutea ), Zimmermannella alba , Zimmermannella bifida ), and Zoogloea caeni ).

In another embodiment, the targeted bacterial cells are those commonly present in the vaginal microflora, including, but not limited to, Acinetobacter antiviralis , Acinetobacter baumannii , Acinetobacter chalcoa ceticus ( Acinetobacter calcoaceticus ), Acinetobacter johnsonii , Actinobaculum massiliense , Actinobaculum schaalii ), Actinomyces europaeus , Actinomyces europaeus ( Actinomyces ) Actinomyces graevenitzii ( Actinomyces graevenitzii ), Actinomyces israelii ( Actinomyces israelii ), Actinomyces meyeri ( Actinomyces meyeri ), Actinomyces naeslundii ( Actinomyces naeslundii ), Actino Myces Neui ( Actinomyces neuii ), Actinomyces odontolyticus ( Actinomyces odontolyticus ), Actinomyces turicensis ( Actinomyces turicensis ), Actinomyces urogenitalis ( Actinomyces urogenitalis ), Actinomyces Viscosus ( Actinomyces viscosus ), Aerococcus christensenii , Aerococcus urinae , Aerococcus viridans ), Aeromonas encheleia ( Aeromonas encheleia ) Salmonisida ( Aeromonas salmonicida ), Afipia massiliensis ( Afipia massiliensis ), Agrobacterium tumefaciens ( Agrobacterium tumefaciens ) ), Algoriphagus aquatilis, Aliivibrio wodanis , Alistipes finegoldii , Alloiococcus otitis , Alloprevotella tannerae ( Alloprevotella tannerae ), Alloscardovia omnicolens , Altererythrobacter epoxidivorans ) , Ammonophilus oxalaticus ), Amnibacterium chiongiense ), Anaerococcus hydrogenalis , Anaerococcus lactolyticus , Anaerococcus murdochii, Anaerococcus obesiensis , Anaerococcus obesiensis Erococcus prevotii ( Anaerococcus prevotii ), Anaerococcus tetradius ( Anaerococcus tetradius ), Anaerococcus vaginalis ( Anaerococcus vaginalis ), Anaeroglobus geminatus ( Anaeroglobus geminatus ), Anoxybacillus Anoxybacillus pushchinoensis , Aquabacterium parvum , Arcanobacterium phocae , Arthrobacter aurescens , Asticcacaulis excentric ), Atopobium minutum ( Atopobium minutum ), Atopobium parbulum ( Atopobiu ) m parvulum ), Atopobium rimae ( Atopobium rimae ) , Atopobium vaginae ( Atopobium vaginae ), Avibacterium gallinarum ), Bacillus acidicola ( Bacillus acidicola ), Bacillus atropaeus ( Bacillus atrophaeus ), Bacillus cereus ( Bacillus cereus ), Bacillus cibi , Bacillus coahuilensis , Bacillus gaemokensis ), Bacillus petanolicus ( Bacillus methanolicus ), Bacillus methanolicus ) ( Bacillus oleronius ), Bacillus pumilus ( Bacillus pumilus ), Bacillus shackletonii ), Bacillus sporothermodurans ), Bacillus subtilis ( Bacillus subtilis ), Bacillus wakoensis ( Bacillus wacoensis ) Weihen stephanensis ( Bacillus weihenstephanensis ), Bacteroides barnesiae ( Bacteroides barnesiae ), Bacteroides coagulans ( Bacteroides coagulans ), Bacteroides dorei ( Bacteroides dorei ), Bacteroides faecis ( Bacteroides faecis ) , Bacteroides forsythus , Bacteroides fragilis , Bacteroides nordii , Bacteroides ovatus , Bacteroides saliercia salyersiae ), Bacteroides sterchoris ( Bacter oides stercoris ), Bacteroides uniformis , Bacteroides vulgatus , Bacteroides xylanisolvens ), Bacteroides zoogleoformans ), Barnesiella viscericola , Bhargavaea cecembensis , Bifidobacterium adolescentis , Bifidobacterium bifidum , Bifidobacterium bre Bifidobacterium breve , Bifidobacterium dentium , Bifidobacterium longum ( Bifidobacterium logum ) subsp. Infantis ( infantis ), Bifidobacterium longum ( Bifidobacterium longum ), Bifidobacterium pseudocatenulatum ( Bifidobacterium pseudocatenulatum ), Bifidobacterium scardovii ( Bifidobacterium scardovii ), Bilophila wadsworth ) , Blautia hydrogenotrophica , Blautia obeum , Blautia producta , Brachybacterium faecium , Bradyrhizobium japonica Cum ( Bradyrhizobium japonicum ), Brevibacterium mcbrellneri ( Brevibacterium mcbrellneri ), Brevibacterium otitidis ( Brevibacterium otitidis ), Brevibacterium paucivorans ( Brevibacterium paucivorans ), Bulleidia extracta ( Bulleidia ) extructa ), Burkholderia fungorum ( Burkholderia fungorum ), Burkholderia phenoliruptix ( Burkholderia phenoliruptix ), Caldicellulosiruptor saccharolyticus ), Caldimonas taiwanensis ( Caldimonas taiwanensis ) Campylobacter gracilis ( Campylobacter gracilis ), Campylobacter hominis ( Campylobacter hominis ), Campylobacter sputorum ( Campylobacter sputorum ), Campylobacter ureolyticus ), Capnocytophaga ochrase ochracea ), Cardiobacterium hominis , Catonella mor Rain ( Catonella morbi ), Chlamydia trachomatis , Chlamydophila abortus , Chondromyces robustus ), Chryseobacterium aquaticum , Chryseobacterium aquaticum ) Lobacter Yungae ( Citrobacter youngae ), Cloacibacterium normanense ( Cloacibacterium normanense ), Clostridium cavendishii , Clostridium colicanis , Clostridium jejuense ) , Clostridium perfringens , Clostridium ramosum , Clostridium sordellii , Clostridium viride , Comamonas terrigena , Corynebacterium acollens ( Corynebacterium accolens ), Corynebacterium appendicis , Corynebacterium coyleae ), Corynebacterium glucuronolyticum ( Corynebacterium glucuronolyticum ), Corynebacterium glutamicum ( Corynebacterium glutamicum ), Corynebacterium jeikeium , Corynebacterium kroppenstedtii , Corynebacterium kroppenstedtii , Corynebacterium lipophiloflavum , Corynebacterium minutiss imum ), Corynebacterium mucifaciens , Corynebacterium nuruki, Corynebacterium pseudogenitalium , Corynebacterium pyrubiciproduscens ( Corynebacterium pyruviciproducens ), Corynebacterium singulare ( Corynebacterium singulare ), Corynebacterium striatum ( Corynebacterium striatum ), Corynebacterium tuberculostearicum ( Corynebacterium xerculostearicum ), Corynebacterium zerosis ), Cryobacterium psychrophilum, Curtobacterium flaccumfaciens , Cutibacterium acnes , Cutibacterium avidum , Cytopaga Illanolytica ( Cytophaga xylanolytica ), Deinococcus radiophilus ( Deinococcus radiophilus ), Delftia tsuruhatensis , Desulfovibrio desulfuricans , Dialister invisus ( Dialister invisus ) Dialister micraerophilus , Dialister pneumosintes , Dialister propionicifaciens , Dickeya chrysanthemi , Dorea longicathena Dorea longicatena ), Eggerthella lenta , Eggerthia catenaformis , Eikenella corrodens , Enhydrobacter aerosaccus , Enterobacter asburiae , Enterobacter asburiae , Enterobacter cloaca ( Enterobacter cloacae ), Enterococcus avium ( Enterococcus avium ), Enterococcus durans ( Enterococcus durans ), Enterococcus faecalis ), Enterococcus faecium ( Enterococcus faecium ), Enterococcus faecium Enterococcus hirae , Erwinia persicina , Erwinia rhapontici , Erwinia toletana , Escherichia coli , Escherichia coli ( Escherichia fergusonii ), Eubacterium brachy ( Eubacterium brachy ) , Eubacterium eligens , Eubacterium nodatum ), Eubacterium rectale ( Eubacterium rectale ), Eubacterium safenum ( Eubacterium saphenum ) , Eubacterium siraeum , Eubacterium sulci , Eubacterium yurii , Exiguobacterium acetylicum , Paklamia Ignaba Facklamia ignava ), Faecalibacterium prausnitzii ), Filifactor alocis ( Filifactor alocis ), Pinegol Dia magna ( Finegoldia magna ), Fusobacterium gonidiaformans ( Fusobacterium gonidiaformans ), Fusobacterium nucleatum ( Fusobacterium nucleatum ), Fusobacterium periodonticum ( Fusobacterium periodonticum ), Gardnerella vaginal Lis ( Gardnerella vaginalis ), Gemella asaccharolytica , Gemella bergeri , Gemella haemolysans , Gemella sanguinis , Thersumophilus Stea Ruth ( Geobacillus stearothermophilus ), Geobacillus thermocatenulatus ( Geobacillus thermocatenulatus ), Geobacillus thermoglucosidasius ), Geobacter grbiciae ( Geobacter grbiciae ), Granulicatella elegans ( elegans) Mophilus ducreyi ( Haemophilus ducreyi ), Haemophilus haemolyticus ( Haemophilus haemolyticus ), Haemophilus parahaemolyticus ( Haemophilus parahaemolyticus ), Haemophilus parainfluenzae ( Haemophilus parainfluenzae ), Hafnia albei ( Hafnia alvei ), Halomonas meridiana ( Halomonas meridiana ), Halomonas phoceae ), Halomonas venusta ( Halomonas venusta ), Herbaspirillum seropedicae , ( Janthinobacterium libidum ), Jonquetella anthropi ), Klebsiella granulomatis , Klebsiella oxytoca , Klebsiella pneumoniae , Lactobacillus acidophilus , Lactobacillus acidophilus Amyloborus ( Lactobacillus amylovorus ), Lactobacillus brevis ( Lactobacillus brevis ), Lactobacillus coleohominis ( Lactobacillus coleohominis ), Lactobacillus crispatus ( Lactobacillus crispatus ), Lactobacillus curvatus , Lactobacillus curvatus ( Lactobacillus curvatus ) ( Lactobacillus delbrueckii ), Lactobacillus fermentum ( Lactobacillus fermentum ), Lactobacillus gasseri , Lactobacillus helveticus ), Lactobacillus inus ense , ni lactobacillus Lactobacillus johnsonii ( Lactobacillus johnsonii ), Lactobacillus kalixensis ( Lactobacillus kalixensis ), Lactobacillus kefiranofaciens ( Lactobacillus kefiranofaciens ), Lactobacillus kimchicus ( Lactobacillus kimchicus ), Lactobacillus kimchicus ( kimactobacillus kit ) Lactobacillus mucosae ( Lactobacillus mucosae ), Lactobacillus panis ( Lactobacillus panis ), Lactobacillus paracasei ( Lactobacillus paracasei ), Lactobacillus plantarum ( Lactobacillus plantaru ) m ), Lactobacillus pontis ( Lactobacillus pontis ), Lactobacillus reuteri ( Lactobacillus reuteri ), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus salivarius ( Lactobacillus sisalivarius ), Lactobacillus salivarius (Lactobacillus sisalivarius), Bacillus vaginalis ( Lactobacillus vaginalis ), Lactococcus lactis , Leptotrichia buccalis , Leuconostoc carnosum , Leuconostoc citreum , Leuconostoc ) Konostoc garlicum ( Leuconostoc garlicum ), Leuconostoc lactis ( Leuconostoc lactis ), Leuconostoc mesenteroides ( Leuconostoc mesenteroides ), Lysinimonas kribbensis ), Mageeibacillus indolicus ( Mageeibacillus indolicus ) ), Maribacter orientalis , Marinomonas protea , Marinospirillum insulare , Massilia timonae , Megasphaera elsdenii Megasphaera micronuciformis , Mesorhizobium amorphae , Methylobacterium radiotolerans , Methylotenera versatilis , Microbacteria Halophyllum ( Microbacte ) rium halophilum ), Micrococcus luteu ), Microterricola viridarii ), Mobiluncus curtisii ), Mobiluncus mulieris ( Mobiluncus mulieris ), Mosquitobacterium thymidum ( Mogibacterium timidum ), Moorella glycerini , Moraxella osloensis , Morganella morganii , Moryella indoligenes , Murdochiella assakaroli asaccharolytica ), Mycoplasma alvi, Mycoplasma genitalium , Mycoplasma hominis , Mycoplasma muris , Mycoplasma salivarium , Mycoplasma salivarium Caucus succinic borans (Negativicoccus succinicivorans ), Neisseria flava , Neisseria gonorrhoeae , Neisseria mucosa ( Neisseria mucosa ), Neisseria subflava ( Neisseria subflava ) , Nevskia ramosa ( Nevskia ramosa ), Nevskia soli ( Nevskia soli ), Nitriliruptor alkaliphilus ( Nitriliruptor alkaliphilus ), Odoribacter splanchnicus ), Oligella urethralis ( Oligella urethralis ) ), Olsenella uli , Paenibacillus Amylolyticus ( Paenibacillus amylolyticus ) , Paenibacillus humicus ), Paenibacillus pabuli ( Paenibacillus pabuli ), Paenibacillus pasadenensis ( Paenibacillus pasadenensis ), Paenibacillus pini ( Paenibacillus pini ) Enibacillus validus ( Paenibacillus validus ), Pantoea agglomerans ( Pantoea agglomerans ), Parabacteroides merdae ( Parabacteroides merdae ), Paraburkholderia caryophylli ( Paraburkholderia caryophylli ), Paracoccus yeei ( Paracoccus yeei ) ), Parastreptomyces abscessus , Parvimonas micra , Pectobacterium betavasculorum , Pectobacterium carotovorum , Pediococcus acidilactici ( Pediococcus acidilactici ) Pediococcus ethanolidurans ( Pediococcus ethanolidurans ), Pedobacter alluvionis ( Pedobacter alluvionis ), Pedobacter wanjuens ( Pedobacter wanjuense ), Pelomonas aquatica ( Pelomonas aquatica ) Peptococcus niger , Peptoniphilus asaccharolyticus , Peptoniphilus gorbachii , Peptoniphilus harei, Peptoniphilus harei ( Peptoniphilus indolicus ), Peptoniphilus lacrimalis ( Pepto niphilus lacrimalis ), Peptoniphilus massiliensis , Peptostreptococcus anaerobius ) ), Photobacterium angustum , Photobacterium frigidiphilum , Photobacterium phosphoreum , Porphyromonas asaccharolytica, Porphyromonas asaccharolytica , Porphyromonas bennonis ( Porphyromonas bennonis ), Porphyromonas catoniae ( Porphyromonas catoniae ), Porphyromonas endodontalis ( Porphyromonas endodontalis ), Porphyromonas gingivalis , Porphyromonas gingivalis , Porphyromonas some Porphyromonas Porphyromonas uenonis , Prevotella amnii , Prevotella baroniae , Prevotella bergensis , Prevotella bivia, Prevotella bivia Prevotella buccae , Prevotella buccalis , Prevotella colorans , Prevotella copri , Prevotella corporis , Prevotella corporis Botella dentalis ( Prevotella den ) talis ), Prevotella denticola , Prevotella disiens , Prevotella intermedia , Prevotella loescheii , Prevotella marci marshii ), Prevotella melaninogenica , Prevotella micans , Prevotella nigrescens , Prevotella oris , Prevotella pleuriti Dis ( Prevotella pleuritidis ), Prevotella ruminicola ( Prevotella ruminicola ), Prevotella shahii ( Prevotella shahii ), Prevotella stercorea ( Prevotella stercorea ), Prevotella timonensis ( Prevotella timonensis ), Prevotella Veroralis ( Prevotella veroralis ), Propionimicrobium lymphophilum , Proteus mirabilis , Pseudomonas abietaniphila , Pseudomonas abietaniphila , Pseudomonas aeruginosa , Pseudomonas aeruginosa Dali ( Pseudomonas amygdali ), Pseudomonas azotoformans ( Pseudomonas azotoformans ), Pseudomonas chlororaphis , Pseudomonas cuatrocienegasensis ) Pseudomonas fulva ), Pseudomonas Naseudomonas lutea , Pseudomonas mucidolens , Pseudomonas oleovorans , Pseudomonas orientalis, Pseudomonas pseudoseudomonas pseudoseudomonas ( Pseudomonas orientalis ), Pseudomonas pseudoseudomonas . psychrophila ), Pseudomonas putida , Pseudomonas synxantha , Pseudomonas syringae ) Aqua tilis ( Rahnella aquatilis ), Ralstonia pickettii ( Ralstonia pickettii ), Ralstonia solanacearum ( Ralstonia solanacearum ), Raoultella planticola ( Raoultella planticola ), Rhizobacter dauci ( Rhizobacter dauci ), Rhizobium etli , Rhodococcus fascians , Rhodopseudomonas palustris , Roseburia intestinalis , Roseburia inulinivorans ), Rothia mucilaginosa , Ruminococcus bromii , Ruminococcus gnavus, Ruminococcus gnavus , Ruminococcus torques , Sanguibacter chedi A keddieii ), Sediminibacterium salmoneum , Selenomonas bovis , Serratia fonticola , Serratia liquefaciens , Serratia marcescens ( Serratia ) marcescens ), Shewanella algae , Shewanella amazonensis , Shigella boydii , Shigella sonnei , Slackia exigua , Sneathia amnii , Sneathia sanguinegens , Solobacterium moorei , Sorangium cellulosum , Sphingobium amiense , Sphingobium amiense ) Sphingobium japonicum , Sphingobium yanoikuyae , Sphingomonas wittichii , Sporosarcina aquimarina , Staphylococcus Staphylococcus aureus ), Staphylococcus auricularis ), Staphylococcus capitis , Staphylococcus epidermidis ), Staphylococcus hamoliticus ( Staphylococcus ) haemolyticus ), Staphylococcus hominis , Staphylococcus lugdunensis ( Staphylococcus lu ) gdunensis ), Staphylococcus saprophyticus , Staphylococcus schleiferi , Staphylococcus simiae , Staphylococcus simulans Staphylococcus , Staphylococcus warneri , Stenotrophomonas maltophilia , Stenoxybacter acetivorans , Streptococcus agalactiae , Streptococcus agalactiae Cus anginosus ( Streptococcus anginosus ), Streptococcus australis ( Streptococcus australis ), Streptococcus equinus , Streptococcus equinus , Streptococcus Infant, Streptococcus streptolyticus ) ), Streptococcus intermedius ( Streptococcus intermedius ), Streptococcus lutetiensis ( Streptococcus lutetiensis ), Streptococcus marimammalium ( Streptococcus marimammalium ), Streptococcus mitis ( Streptococcus ), Streptococcus mutantococcus ( Streptococcus mutans ), Streptococcus oralis , Streptococcus parasanguinis , Streptococcus phocae , Streptococcus phocae , Streptococcus pseudopneumoniae, Streptococcus pseudopneumoniae Streptococcus salivarius ( Str eptococcus salivarius ), Streptococcus sanguinis , Streptococcus thermophilus , Sutterella wadsworthensis , Tannerella forsythia Tannerella forsythia Aromatic borans ( Terrahaemophilus aromaticivorans ), Treponema denticola ( Treponema denticola ), Treponema maltophilum ( Treponema maltophilum ), Treponema parvum ( Treponema parvum ), Treponema vincentii ( Treponema vincentii ), Trueperella bernardiae ( Trueperella bernardiae ), Turicella otitidis ), Ureaplasma parvum ( Ureaplasma parvum ), Ureaplasma urealyticum ( Ureaplasma urealyticum ), Varibaculum cambriens ( Varibaculum cambriens ) , Variovorax paradoxus , Veillonella atypica , Veillonella dispar , Veillonella montpellierensis , Veillonella parbula ( Veillonella parvula ), Virgibacillus proomii , Viridibacillus arenosi , Viridibacillus arvi , Weissella cibaria , Weissella soli , Xanthomonas campestris ( Xanthomonas campestris ), Xanthomonas vesicatoria , Zobellia laminariae , and Zoogloea ramigera .

In one embodiment, the targeted bacterium is Escherichia coli. In certain embodiments, the targeted bacteria are Shiga-toxin producing E. coli (STEC).

The targeted bacterial cell population may comprise one or several bacteria of interest as defined above. In particular, the targeted bacterial cell population may comprise Escherichia coli and one or several other bacteria of interest as defined above.

Accordingly, the bacteriophage used to prepare the bacterial delivery vehicle, and then the bacterial delivery vehicle, target bacterial cells from any one or more of the aforementioned genera and/or species of bacteria to specifically deliver the payload of interest. (eg, specifically targeting).

In one embodiment, the targeted bacterium is a pathogenic bacterium. The targeted bacteria may be virulent bacteria.

Targeted bacteria include extended spectrum beta-lactamase-producing (ESBL) Escherichia coli, ESBL Klebsiella pneumoniae, vancomycin-resistant Enterococcus (VRE), methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant (MDR) Acinetobacter baumanni, MDR Enterobacter spp., and combinations thereof. The targeted bacterium may be selected from the group consisting of an extended-spectrum beta-lactamase-producing (ESBL) Escherichia coli strain.

Alternatively, the targeted bacteria may be bacteria of a given species of microbiome, including bacteria of the human microbiota.

The present disclosure relates to a bacterial delivery vehicle containing a payload as described herein. Bacterial delivery vehicles are prepared from bacterial viruses. The bacterial delivery vehicle is selected so as to be able to introduce the payload into the targeted bacteria.

Bacterial viruses from which the bacterial delivery vehicles disclosed herein can be derived include bacteriophages. Optionally, the bacteriophage is based on the taxonomy of [Krupovic et al, Arch Virol, 2015], Myoviridae family, Podoviridae family, Siphoviridae family, and Akkermanviridae ( It is selected from the order Caudovirales, which consists of the family Ackermannviridae.

Bacteriophages are from the Myoviridae family (such as, but not limited to, genus Cp220virus, Cp8virus, Ea214virus, Felixo1virus, Mooglevirus, Suspvirus, Hp1virus, P2virus, Kaivirus, P100virus, Sylviavirus, Sporovirus). 1 virus, Tsar bomba virus, Twit virus, Cc31 virus, Jd18 virus, Js98 virus, Kp15 virus, Moon virus, Rb49 virus, Rb69 virus, S16 virus, Skizot 4 virus, Sp18 virus, T4 virus, Cr3 virus, Se1 Virus, V5 virus, Avou virus, Agate virus, Agrican 357 virus, Ap22 virus, Arv1 virus, B4 virus, Bastille virus, Bc431 virus, Bcep78 virus, Bicepmu virus, Biquartavirus, Bxz1 virus, Cd119 virus, Cp51 virus, Cvm10 virus, Eah2 virus, El virus, Hapuna virus, Zimmer virus, Kpp10 virus, M12 virus, Machina virus, Marsha virus, Msw3 virus, Mu virus, Myohalovirus, Nit1 virus, P1 virus, Parkfuna virus, Pbunavirus, Phikzvirus, Rheph4virus, Rsl2virus, Rslunavirus, Secunda5virus, Sep1virus, Spn3virus, Sbunavirus, Tglvirus, Vhmlvirus and Wphvirus).

Bacteriophages are from the Podoviridae family (e.g., without limitation, genus Fri1 virus, Kp32 virus, Kp34 virus, Phikmv virus, Prado virus, Sp6 virus, T7 virus, Cp1 virus, P68 virus, Phi29 virus, Nona 33 virus, Pocj virus, Tl2011 virus, Bcep22 virus, Bpp1 virus, Cba41 virus, Dfl12 virus, Ea92 virus, Epsilon 15 virus, F116 virus, G7c virus, Zalpa virus, Kf1 virus, Kpp25 virus, Ret1 virus, Rouge 24 virus, Rouge 7 virus, N4 virus, nonanavirus, P22 virus, page virus, pieco32 virus, Prtb virus, Sp58 virus, Una 961 virus and Vp5 virus).

Bacteriophages are from the family Cypoviridae (such as, but not limited to, the genus Camvirus, Ricavirus, R4virus, Arcadianvirus, Coopervirus, Pg1virus, Pipefishvirus, Rosebushvirus, Bruhitavirus, Che9cvirus, Hawkeye virus). , Plot virus, Jersey virus, K1g virus, Sp31 virus, Lmd1 virus, Una 4 virus, Bongo virus, Ray virus, Butters virus, Charlie virus, Ready virus, Baxter virus, Nympadora virus, Vignuz virus, Fishbourne Virus, Pion's virus, Kp36 virus, Loge 1 virus, Rtp virus, T1 virus, Tls virus, Ab18 virus, Amigo virus, Anatole virus, Andromeda virus, Artis virus, Banyad virus, Bernal 13 virus, Biceptima virus, Bron virus, C2 virus, C5 virus, Cba181 virus, Cbast virus, cecivirus, somatic 8 virus, chi virus, Cjw1 virus, condock virus, Kronus virus, D3112 virus, D3 virus, decurovirus, demosthenes virus, two sets Virus, E125 virus, Eiau virus, Ff47 virus, Gaia virus, Giles virus, Gordon virus, Gordtnk virus, Harrison virus, Hk578 virus, Hk97 virus, Genst virus, Jwx virus, Kellegiovirus, Coravirus, L5 virus, Lambda virus, Laroyer virus, Ryphi virus, Marvin virus, Mudcat virus, N15 virus, Nonag virus, Np1 virus, Omega virus, P12002 virus, P12024 virus, P23 virus, P70 virus, Pa6 virus, Pamx74 virus, Paience virus , Pbi1 virus, Pepy6 virus, Pfr1 virus, Phic31 virus, Phicbk virus, Pietavirus, Pipeel virus, Phijl1 virus, Pis4a virus, Psa virus, Cymuna virus, Rdjl virus, Rer2 virus, Sap6 virus, Send 513 virus, Septima 3 virus, Seurat virus, Sextag virus, Sfi11 virus, Sfi21dt1 virus, Cytara virus, Sk1 virus, Slash virus, Smoothie virus, Sups virus, Sp beta virus, Ssp2 virus, T5 virus, Tank virus, Teen 2 Virus, Titan virus, Tm4 virus, Tp21 virus, Tp84 virus, Tria virus, Trigintaduovirus, Vegas virus, Vendettavirus, Wbetavirus, Wildcat virus, Wizard virus, Us virus, Xp10 virus, Ydn12 virus and Wia virus ) can be selected from

The bacteriophage may be selected from the family Ackermanviridae (eg, without limitation, the genus Ag3virus, Lymestonevirus, Cba120virus and Vi1virus).

Optionally, the bacteriophage is not part of the order Caudovirales, but in the family of an unspecified order, such as, without limitation, the Tectiviridae family (such as the genera alphatactiviruses, betatectiviruses), the Corticoviridae family ( For example genus corticovirus), Inoviridae family (eg genus fibrovirus, havenivirus, inovirus, lineavirus, plectrovirus, saetivirus, vespertiliovirus), cystoviridae (Cystoviridae) family (eg genus cystovirus), Leviviridae family (eg genus Allorevivirus, levivirus), Microviridae family (eg genus alpha3microvirus, G4microvirus, Phix174 microviruses, bdelomicroviruses, chlamydia microviruses, spiromicroviruses) and from the family Plasmaviridae (such as the genus Plasmaviruses).

Optionally, the bacteriophage is not part of the order Caudovirales, but in the family of an unspecified order, such as, without limitation, Ampullaviridae, FuselloViridae, Globuloviridae, Gutaviridae ( Guttaviridae), Lipothrixviridae, Pleolipoviridae, Rudiviridae, Salterprovirus and Bicaudaviridae.

A non-limiting list of bacterial genera and their known host-specific bacterial viruses is presented in the following paragraphs. The chimeric RBP and/or branched RBP and/or recombinant gpJ protein and/or recombinant gpH protein, and bacterial delivery vehicle disclosed herein, as non-limiting examples, can be engineered from the following phage. Synonyms and spelling variations are indicated in parentheses. Homonyms are repeated as frequently as they exist (eg, D, D, d). Unnamed phages are marked with "NN" next to their insides and their numbers are given in parentheses.

Bacteria of the genus Actinomyces can be infected by the following phages: Av-I, Av-2, Av-3, BF307, CT1, CT2, CT3, CT4, CT6, CT7, CT8 and 1281.

Bacteria of the genus Aromonas can be infected by the following phages: AA-I, Aeh2, N, PMl, TP446, 3, 4, 11, 13, 29, 31, 32, 37, 43, 43-10T, 51, 54, 55R.1, 56, 56RR2, 57, 58, 59.1, 60, 63, Aehl, F, PM2, 1, 25, 31, 40RR2.8t, (syn=44R), (syn=44RR2.8t), 65, PM3, PM4, PM5 and PM6.

Bacteria of the genus Bacillus can be infected by the following phages: A, aizl, Al-K-I, B, BCJAl, BCl, BC2, BLLl, BLl, BP142, BSLl, BSL2, BSl, BS3, BS8, BS15, BS18, BS22 , BS26, BS28, BS31, BS104, BS105, BS106, BTB, B1715V1, C, CK-I, Coll, Corl, CP-53, CS-I, CSi, D, D, D, D5, entl, FP8, FP9 , FSi, FS2, FS3, FS5, FS8, FS9, G, GH8, GT8, GV-I, GV-2, GT-4, g3, gl2, gl3, gl4, gl6, gl7, g21, g23, g24, g29 , H2, kenl, KK-88, Kuml, Kyul, J7W-1, LP52, (syn=LP-52), L7, Mexl, MJ-I, mor2, MP-7, MP10, MP12, MP14, MP15, Neol , N°2, N5, N6P, PBCl, PBLA, PBPl, P2, S-a, SF2, SF6, Shal, Sill, SP02, (syn= ΦSPP1), SPβ, STI, STi, SU-Il, t, TbI, Tb2 , Tb5, TbIO, Tb26, Tb51, Tb53, Tb55, Tb77, Tb97, Tb99, Tb560, Tb595, Td8, Td6, Tdl5, TgI, Tg4, Tg6, Tg7, Tg9, TgIO, TgIl, Tgl3, Tgl5, Tg21, Tgl5 , Tin7, Tin8, Tinl3, Tm3, Tocl, Togl, toll, TP-I, TP-10vir, TP-15c, TP-16c, TP-17c, TP-19, TP35, TP51, TP-84, Tt4, Tt6 , A type, B type, C type, D type, E type, Tφ3, VA-9, W, wx23, wx26, Yunl, α, γ, pl l,, φmed-2, φT, φμ-4, φ3T, φ75, φlO5, (syn= φlO5), IA, IB, 1-97A, 1-97B, 2, 2, 3 , 3, 3, 5, 12, 14, 20, 30, 35, 36, 37, 38, 41C, 51, 63, 64, 138D, I, II, IV, NN-Bacillus (13), alel, ARl, AR2, AR3, AR7, AR9, Bace-11, (syn=11), Bastille, BL1, BL2, BL3, BL4, BL5, BL6, BL8, BL9, BP124, BS28, BS80, Ch, CP-51, CP- 54, D-5, darl, denl, DP-7, entl, FoSi, FoS2, FS4, FS6, FS7, G, gall, gamma, GEl, GF-2, GSi, GT-I, GT-2, GT- 3, GT-4, GT-5, GT-6, GT-7, GV-6, gl5, 19, 110, ISi, K, MP9, MP13, MP21, MP23, MP24, MP28, MP29, MP30, MP32, MP34, MP36, MP37, MP39, MP40, MP41, MP43, MP44, MP45, MP47, MP50, NLP-I, No.l, N17, N19, PBSl, PKl, PMBl, PMB12, PMJl, S, SPOl, SP3, SP5, SP6, SP7, SP8, SP9, SP10, SP-15, SP50, (syn= SP-50), SP82, SST, subl, SW, Tg8, Tgl2, Tgl3, Tgl4, thul, thuΛ, thuS, Tin4, Tin23, TP-13, TP33, TP50, TSP-I, Type V, Type VI, V, Vx, β22, φe, φNR2, φ25, φ63, 1, 1, 2, 2C, 3NT, 4, 5, 6, 7, 8, 9, 10, 12, 12, 17, 18, 19, 21, 138, III, 4 (B. megateriwn), 4 (B. sphaericus)), AR13, BPP-IO, BS32, BS107, Bl, B2, GA-I, GP-IO, GV-3 , GV-5, g8, MP20, MP27, MP49, Nf, PP5, PP6, SF5, Tgl8, TP-I, Versailles, φl5, φ29, 1-97, 837/IV, mi-Bacillus (1), BatlO, BSlO, BSLI l, BS6, BSI l, BS16, BS23, BSlOl, BS102, gl8, morl, PBLl, SN45, thu2, thu3, TmI, Tm2, TP-20, TP21, TP52, Type F, Type G, Type IV , HN-BacMus (3), BLE, (syn=θc), BS2, BS4, BS5, BS7, BlO, B12, BS20, BS21, F, MJ-4, PBA12, AP50, AP50-04, AP50-11, AP50-23, AP50-26, AP50-27 and Bam35. The following Bacillus-specific phages are defective: DLP10716, DLP-11946, DPB5, DPB12, DPB21, DPB22, DPB23, GA-2, M, No. IM, PBLB, PBSH, PBSV, PBSW, PBSX, PBSY, PBSZ, phi, SPa, type 1 and μ.

Bacteria of the genus Bacteriodes can be infected by the following phages: ad I2, Baf-44, Baf-48B, Baf-64, Bf-I, Bf-52, B40-8, Fl, βl, φAl, φBrOl, φBrO2 , 11, 67.1, 67.3, 68.1, mt-Bacteroides (3), Bf42, Bf71, HN-Bdelobvibrio (1) and BF-41.

Bacteria of the genus Bordetella can be infected by the following phages: 134 and NN-Bordetella (3).

Bacteria of the genus Borrelia can be infected by the following phages: NN-Borrelia (1) and NN-Borrelia (2).

Bacteria of the genus Brucella can be infected by the following phages: A422, Bk, (syn=Berkeley), BM29, FOi, (syn=FOl), (syn=FQl), D, FP2, (syn=FP2), ( syn= FD2), Fz, (syn= Fz75/13), (syn= Florence 75/13), (syn= Fi), Fi, (syn= Fl), Fim, (syn= FIm), (syn= Fim ), FiU, (syn= FlU), (syn= FiU), F2, (syn= F2), F3, (syn= F3), F4, (syn= F4), F5, (syn= F5), F6, F7, (syn= F7), F25, (syn= F25), (syn= ￡25), F25U, (syn= F25u), (syn= F25U), (syn= F25V), F44, (syn-F44) , F45, (syn= F45), F48, (syn= F48), I, Im, M, MC/75, M51, (syn= M85), P, (syn= D), S708, R, Tb, ( syn= TB), (syn= Tbilisi), W, (syn= Wb), (syn= waybridge), X, 3, 6, 7, 10/1, (syn= 10), (syn= F8), (syn= F8), 12m, 24/11, (syn= 24), (syn= F9), (syn= F9), 45/111, (syn= 45), 75, 84, 212/XV, (syn = 212), (syn=Fi0), (syn=FlO), 371/XXIX, (syn=371), (syn=Fn), (syn=Fll) and 513.

Bacteria of the genus Burkholderia can be infected by the following phages: CP75, NN-Burkholderia (1) and 42.

Bacteria of the genus Campylobacter can be infected by the following phages: type C, NTCC12669, NTCC12670, NTCC12671, NTCC12672, NTCC12673, NTCC12674, NTCC12675, NTCC12676, NTCC12677, NTCC12678, NTCC12679, NTCC12680, NTCC12681, NTCC12682, NTCC12683, NTCC12684, 32f, 111c, 191, NN-Campylobacter (2), Vfi-6, (syn= V19), VfV-3, V2, V3, V8, V16, (syn= Vfi-1), V19, V20 (V45) ), V45, (syn=V-45) and NN-Campylobacter (1).

Bacteria of the genus Chlamydia can be infected by the following phages: Chpl.

Bacteria of the genus Clostridium can be infected by the following phages: CAKl, CA5, Ca7, CEβ, (syn= 1C), CEγ, Cldl, c-n71, c-203 Tox-, DEβ, (syn= ID) , (syn= lDt0X+), HM3, KMl, KT, Ms, NAl, (syn= Naltox+), PA135Oe, Pfo, PL73, PL78, PL81, Pl, P50, P5771, P19402, lCt0X+, 2Ct0X\ 2D3 (syn= 2Dt0X+ ), 3C, (syn= 3Ctox+), 4C, (syn= 4Ct0X+), 56, III-l, NN-Clostridium (61), NBlt0X+, αl, CAl, HMT, HM2, PFl5 P-23, P- 46, Q-05, Q-oe, Q-16, Q-21, Q-26, Q-40, Q-46, S111, SA02, WA01, WA03, Wm, W523, 80, C, CA2, CA3, CPT1, CPT4, cl, c4, c5, HM7, H11/A1, H18/Ax, FWS23, Hi58ZA1, K2ZA1, K21ZS23, ML, NA2t0X; Pf2, Pf3, Pf4, S9ZS3, S41ZA1, S44ZS23, α2, 41, 112ZS23, 214/S23, 233/Ai, 234/S23, 235/S23, II-l, II-2, II-3, NN-Clost Iridium (12), CAI, Fl, K, S2, 1, 5 and NN-clostridium (8).

Bacteria of the genus Corynebacterium can be infected by the following phages: CGKl (defective), A, A2, A3, AlOl, A128, A133, A137, A139, A155, A182, B, BF, B17, B18, B51, B271, B275, B276, B277, B279, B282, C, capi, CCl, CGl, CG2, CG33, CL31, Cog, (syn=CG5), D, E, F, H, H-I, hqi, hq2, 11ZH33, Ii/31, J, K, K, (syn= Ktox"), L, L, (syn= Ltox+), M, MC-I, MC-2, MC-3, MC-4, MLMa, N , O, ovi, ov2, ov3, P, P, R, RP6, RS29, S, T, U, UB1, ub2, UH1, UH3, uh3, uh5, uh6, β, (syn= βtox+), βhv64, βvir , γ, (syn= γtoχ-), γl9, δ, (syn= δ'ox+), p, (syn= ptoχ-), Φ9, φ984, ω, IA, 1/1180, 2, 2/1180, 5 /1180, 5ad/9717, 7/4465, 8/4465, 8ad/10269, 10/9253, 13Z9253, 15/3148, 21/9253, 28, 29, 55, 2747, 2893, 4498 and 5848.

Bacteria of the genus Enterococcus can be infected by the following phages: DF78, Fl, F2, 1, 2, 4, 14, 41, 867, Dl, SB24, 2BV, 182, 225, C2, C2F, E3, E62 , DS96, H24, M35, P3, P9, SBlOl, S2, 2BII, 5, 182a, 705, 873, 881, 940, 1051, 1057, 21096C, NN-Enterococcus (1), PEl, Fl, F3, F4, VD13, 1, 200, 235 and 341.

Bacteria of the genus Erysipelottrix can be infected by the following phages: NN-acepelottrix (1).

Bacteria of the genus Escherichia can be infected by the following phages: BW73, B278, D6, D108, E, El, E24, E41, FI-2, FI-4, FI-5, HI8A, Ffl8B, i, MM , Mu, (syn= mu), (syn= MuI), (syn= Mu-I), (syn= MU-I), (syn= MuI), (syn= μ), 025, PhI-5, Pk , PSP3, Pl, PlD, P2, P4 (faulty), Sl, Wφ, φK13, φR73 (defective), φl, φ2, φ7, φ92, ψ (defective), 7 A, 8φ, 9φ, 15 ( defect), 18, 28-1, 186, 299, HH-Escherichia (2), AB48, CM, C4, C16, DD-VI, (syn= Dd-Vi), (syn= DDVI), ( syn=DDVi), E4, E7, E28, FIl, FI3, H, Hl, H3, H8, K3, M, N, ND-2, ND-3, ND4, ND-5, ND6, ND-7, Ox -I (syn= OXl), (syn= HF), Ox-2 (syn= 0x2), (syn= 0X2), Ox-3, Ox-4, Ox-5, (syn= 0X5), Ox-6 , (syn=66F), (syn=φ66t), (syn=φ66t-)5 0111, PhI-I, RB42, RB43, RB49, RB69, S, SaI-I, Sal-2, Sal-3, Sal- 4, Sal-5, Sal-6, TC23, TC45, TuII*-6, (syn= TuII*), TuIP-24, TuII*46, TuIP-60, T2, (syn= ganuTia), (syn= γ ), (syn= PC), (syn= P.C.), (syn= T-2), (syn= T2), (syn= P4), T4, (syn= T-4), (syn= T4), T6, T35, αl, 1, IA, 3, (syn= Ac3), 3A, 3T+, (syn= 3), (syn= Ml), 5φ, (syn= φ5), 9266Q, CFO103, HK620, J, K, KlF, m59, no. A, no. E, no. 3, no. 9, N4, sd, (syn= Sd), (syn= SD), (syn= Sa)3 (syn= sd), (syn= SD), (syn= CD), T3, (syn= T-3 ), (syn= T3), T7, (syn= T-7), (syn= T7), WPK, W31, ΔH, φC3888, φK3, φK7, φK12, φV-1, Φ04-CF, Φ05, Φ06, φ07, φl, φl.2, φ20, φ95, φ263, φ1092, φl, φll, (syn=φW), Ω8, 1, 3, 7, 8, 26, 27, 28-2, 29, 30, 31, 32, 38, 39, 42, 933W, NN-Escherichia (1), Esc-7-11, AC30, CVX-5, Cl, DDUP, ECl, EC2, E21, E29, Fl, F26S, F27S, Hi , HK022, HK97, (syn= ΦHK97), HK139, HK253, HK256, K7, ND-I, no. D, PA-2, q, S2, Tl, (syn= α), (syn= P28), ( syn= T-I), (syn= Tx), T3C, T5, (syn= T-5), (syn= T5), UC-I, w, β4, γ2, λ (syn= lambda), (syn= Φλ) ), ΦD326, φγ, Φ06, Φ7, Φ10, φ80, χ, (syn= χi), (syn= χ), (syn= χi), 2, 4, 4A, 6, 8A, 102, 150, 168, 174, 3000, AC6, AC7, AC28, AC43, AC50, AC57, AC81, AC95, HK243, KlO, ZG/3A, 5, 5A, 21EL, H19-J and 933H.

Bacteria of the genus Fusobacterium can be infected by the following phages: NN-Fusobacterium (2), fv83-554/3, fv88-531/2, 227, fv2377, fv2527 and fv8501.

Bacteria of the genus Haemophilus can be infected by the following phages: HPl, S2 and N3.

Bacteria of the genus Helicobacter can be infected by the following phages: HPl and ^-Helicobacter (1).

Bacteria of the genus Klebsiella can be infected by the following phages: AIO-2, KI4B, Kl6B, Kl9, (syn=K19), Kl14, Kl15, Kl21, Kl28, Kl29, KI32, Kl33, Kl35, Kl106B, Kl171B. , Kl181B, Kl832B, AIO-I, AO-I, AO-2, AO-3, FC3-10, K, Kl1, (syn= KIl), Kl2, (syn= K12), Kl3, (syn= K13) , (syn=Kl 70/11), Kl4, (syn=K14), Kl5, (syn=K15), Kl6, (syn=K16), Kl7, (syn=K17), Kl8, (syn=K18), Kl19, (syn=K19), Kl27, (syn=K127), Kl31, (syn=K131), Kl35, Kl171B, II, VI, IX, CI-I, Kl4B, Kl8, Kl11, Kl12, Kl13, Kl16, Kl17, Kl18, Kl20, Kl22, Kl23, Kl24, Kl26, Kl30, Kl34, Kl106B, KIi65B, Kl328B, KLXI, K328, P5046, 11, 380, III, IV, VII, VIII, FC3-11, Kl2B, (syn = K12B), Kl25, (syn= K125), Kl42B, (syn= K142), (syn= K142B), Kl181B, (syn= KIl 81), (syn= K1181B), Kl765/!, (syn= K1765/ 1), Kl842B, (syn=K1832B), Kl937B, (syn=K1937B), Ll, φ28, 7, 231, 483, 490, 632 and 864/100.

Bacteria of the genus Lepitospira can be infected by the following phages: LEl, LE3, LE4 and NN-Leptospira (1).

Bacteria of the genus Listeria can be infected by the following phages: A511, 01761, 4211, 4286, (syn=BO54), A005, A006, A020, A500, A502, A511, Al 18, A620, A640, B012, B021, B024, B025, B035, B051, B053, B054, B055, B056, BlOl, BI 10, B545, B604, B653, C707, D441, HSO47, HOG, H8/73, H19, H21, H43, H46, H107, H108 , HI 10, H163/84, H312, H340, H387, H391/73, H684/74, H924A, PSA, U153, φMLUP5, (syn= P35), 00241, 00611, 02971A, 02971C, 5/476, 5/ 911, 5/939, 5/11302, 5/11605, 5/11704, 184, 575, 633, 699/694, 744, 900, 1090, 1317, 1444, 1652, 1806, 1807, 1921/959, 1921/ 11367, 1921/11500, 1921/11566, 1921/12460, 1921/12582, 1967, 2389, 2425, 2671, 2685, 3274, 3550, 3551, 3552, 4276, 4277, 4292, 4477, 5337, 5348/11363, 5348/11646, 5348/12430, 5348/12434, 10072, 11355C, 11711A, 12029, 12981, 13441, 90666, 90816, 93253, 907515, 910716 and NN-Lisperia (15).

Bacteria of the genus Morganella can be infected by the following phages: 47.

Bacteria of the genus Mycobacterium can be infected by the following phages: 13, AG1, ALi, ATCC 11759, A2, B.C3, BG2, BK1, BK5, Butyricum, B-I, B5, B7, B30, B35, Clark, Cl, C2, DNAIII, DSP1, D4, D29, GS4E, (syn=GS4E), GS7, (syn=GS-7), (syn=GS7), IPa, Lacticola, Legendre, Leo, L5, (syn= ΦL-5), MC-I, MC-3, MC-4, Minetti, MTPHI 1, Mx4, MyF3P/59a, phlei, (syn= phlei 1), phlei 4, Poronus II, Rabinowitzky , Smegmatis, TM4, TM9, TM10, TM20, Y7, Y10, φ630, IB, IF, IH, 1/1, 67, 106, 1430, Bl, (syn= Bol), B24, D, D29, F-K , F-S, HP, Polonus I, Roy, Rl, (syn=Rl-Myb), (syn=Ri), 11, 31, 40, 50, 103a, 103b, 128, 3111-D, 3215-D and NN -Mycobacterium (1).

Bacteria of the genus Neisseria can be infected by the following phages: Group I, Group II and NPl.

Bacteria of the genus Nocardia can be infected by the following phages: MNP8, NJ-L, NS-8, N5 and TtiN-Nocardia.

Bacteria of the genus Proteus can be infected by the following phages: Pm5, 13vir, 2/44, 4/545, 6/1004, 13/807, 20/826, 57, 67b, 78, 107/69, 121, 9 /0, 22/608, 30/680, PmI, Pm3, Pm4, Pm6, Pm7, Pm9, PmIO, PmIl, Pv2, πl, φm, 7/549, 9B/2, 10A/31, 12/55, 14 , 15, 16/789, 17/971, 19A/653, 23/532, 25/909, 26/219, 27/953, 32A/909, 33/971, 34/13, 65, 5006M, 7480b, VI , 13/3a, Clichy 12, π2600, φχ7, 1/1004, 5/742, 9, 12, 14, 22, 24/860, 2600/D52, Pm8 and 24/2514.

Bacteria of the genus Providencia can be infected by the following phages: PL25, PL26, PL37, 9211/9295, 9213/921 Ib, 9248, 7/R49, 7476/322, 7478/325, 7479, 7480, 9000/ 9402 and 9213/921 Ia.

Bacteria of the genus Pseudomonas can be infected by the following phages: PfI, (syn=Pf-I), Pf2, Pf3, PP7, PRRl, 7s, im-Pseudomonas (1), AI-I, AI-2, B 17 , B89, CB3, Col 2, Col 11, Col 18, Col 21, C154, C163, C167, C2121, E79, F8, ga, gb, H22, K1, M4, N2, Nu, PB-I, (syn= PBl), pfl6, PMN17, PPl, PP8, Psal, PsPl, PsP2, PsP3, PsP4, PsP5, PS3, PS17, PTB80, PX4, PX7, PYOl, PYO2, PYO5, PYO6, PYO9, PYO14, PYO13, PYO16 , PYO18, PYO19, PYO20, PYO29, PYO32, PYO33, PYO35, PYO36, PYO37, PYO38, PYO39, PYO41, PYO42, PYO45, PYO47, PYO48, PYO64, PYO69, PYO103, PlK, SLPl, SL2, S2, UNL-I , wy, Yai, Ya4, Yan, φBE, φCTX, φC17, φKZ, (syn=ΦKZ), φ-LT, Φmu78, φNZ, φPLS-1, φST-1, φW-14, φ-2, 1/72 , 2/79, 3, 3/DO, 4/237, 5/406, 6C, 6/6660, 7, 7v, 7/184, 8/280, 9/95, 10/502, 11/DE, 12 /100, 12S, 16, 21, 24, 25F, 27, 31, 44, 68, 71, 95, 109, 188, 337, 352, 1214, HN-Pseudomonas (23), A856, B26, CI-I, CI-2, C5, D, gh-1, Fl 16, HF, H90, K5, K6, Kl 04, K109, K166, K267, N4, N5, O6N-25P, PE69, Pf, PPN25, PPN35, PPN89, PPN91, PP2, PP3, PP4, PP6, PP7, PP8, PP56, PP87, PPl 14, PP206, PP207, PP306, PP651, Psp231a, Pssy401, Pssy9220, psi, PTB2, PTB20, PTB42, PXl, PX3, PX10, PX12, PX14, PYO70, PYO71, R, SH6, SH133, Ya7, Ya5, Ya7 , φBS, ΦKf77, φ-MC, ΦmnF82, φPLS27, φPLS743, φS-1, 1, 2, 2, 3, 4, 5, 6, 7, 7, 8, 9, 10, 11, 12, 12B, 13 , 14, 15, 14, 15, 16, 17, 18, 19, 20, 20, 21, 21, 22, 23, 23, 24, 25, 31, 53, 73, 119x, 145, 147, 170, 267 , 284, 308, 525, NN-pseudomonas (5), af, A7, B3, B33, B39, BI-I, C22, D3, D37, D40, D62, D3112, F7, FlO, g, gd, ge, gξHwl2, Jb 19, KFl, L°, OXN-32P, O6N-52P, PCH-I, PC13-1, PC35-1, PH2, PH51, PH93, PH132, PMW, PM13, PM57, PM61, PM62, PM63, PM69, PM105, PMl 13, PM681, PM682, PO4, PPl, PP4, PP5, PP64, PP65, PP66, PP71, PP86, PP88, PP92, PP401, PP711, PP891, Pssy41, Pssy42, Pssy403, Pssy420, Pssy404, Pssy420 , PS4, PS-IO, Pz, SD1, SL1, SL3, SL5, SM, φC5, φCl1, φCl1-1, φC13, φC15, φMO, φX, φO4, φll, φ240, 2, 2F, 5, 7m , 11, 13, 13/441, 14, 20, 24, 40, 45, 49, 61, 73, 148, 160, 198, 218, 222, 236, 242, 246, 249, 258, 269, 295, 297 , 309 , 318, 342, 350, 351, 357-1, 400-1, HN-Pseudomonas (6), GlOl, M6, M6a, Ll, PB2, Pssyl5, Pssy4210, Pssy4220, PYO12, PYO34, PYO49, PYO50, PYO51, PYO52, PYO53, PYO57, PYO59, PYO200, PX2, PX5, SL4, φO3, φO6 and 1214.

Bacteria of the genus Rickettsia can be infected by the following phages: NN-rickettia.

Bacteria of the genus Salmonella can be infected by the following phages: b, Beckles, CT, d, Dundee, f, FeIs 2, GI, GUI, GVI, GVIII, k, K, i, j, L, 01, ( syn= 0-1), (syn= O1), (syn= O-I), (syn= 7), 02, 03, P3, P9a, PlO, Sab3, Sab5, SanlS, Sanl7, SI, tonton, ViI, ( syn= ViI), 9, im Salmonella (1), N-I, N-5, N-IO, N-17, N-22, 11, 12, 16-19, 20.2, 36, 449C/C178, 966A/C259 , a, B.A.O.R., e, G4, GUI, L, LP7, M, MG40, N-18, PSA68, P4, P9c, P22, (syn=P22), (syn=PLT22), (syn=PLT22), P22al , P22-4, P22-7, P22-11, SNT-I, SNT-2, SP6, Villi, ViIV, ViV, ViVI, ViVII, Worksop, Sj5, ε34, 1,37, 1(40), (syn = φl[40]), 1,422, 2, 2.5, 3b, 4, 5, 6,14(18), 8, 14(6,7), 10, 27, 28B, 30, 31, 32, 33, 34 , 36, 37, 39, 1412, SNT-3, 7-11, 40.3, c, C236, C557, C625, C966N, g, GV, G5, Gl 73, h, IRA, Jersey, MB78, P22-1, P22-3, P22-12, Sabl, Sab2, Sab2, Sab4, Sanl, San2, San3, San4, San6, San7, San8, San9, Sanl3, Sanl4, Sanl6, Sanl8, Sanl9, San20, San21, San22, San23, San24, San25, San26, SasLl, SasL2, SasL3, SasL4, SasL5, SlBL, SII, ViII, φl, 1, 2, 3a, 3al, 1010, Ym-Salmonella (1), N-4, SasL6 and 27.

Bacteria of the genus Serratia can be infected by the following phages: A2P, PS20, SMB3, SMP, SMP5, SM2, V40, V56, ic, ΦCP-3, ΦCP-6, 3M, 10/la, 20A, 34CC, 34H, 38T, 345G, 345P, 501B, SMB2, SMP2, BC, BT, CW2, CW3, CW4, CW5, Lt232, L2232, L34, L.228, SLP, SMPA, V.43, σ, φCWl, ΦCP6- 1, ΦCP6-2, ΦCP6-5, 3T, 5, 8, 9F, 10/1, 2OE, 32/6, 34B, 34CT, 34P, 37, 41, 56, 56D, 56P, 6OP, 61/6, 74/6, 76/4, 101/8900, 226, 227, 228, 229F, 286, 289, 290F, 512, 764a, 2847/10, 2847/1Oa, L.359 and SMBl.

Bacteria of the genus Shigella can be infected by the following phages: Fsa, (syn=a), FSD2d, (syn=D2d), (syn=W2d), FSD2E, (syn=W2e), fv, F6, f7. 8, H-Sh, PE5, P90, SfII, Sh, SHm, SHrv, (syn=HIV), SHvi, (syn=HVI), SHVvm, (syn=HVIII), SKγ66, (syn=gamma 66), ( syn=yββ), (syn=γ66b), SKm, (syn=SIIIb)5 (syn=UI), SKw, (syn=Siva), (syn=IV), SIC™, (syn=SIVA.), ( syn=IVA), SKvi, (syn=KVI), (syn=Svi), (syn=VI), SKvm, (syn=Svm), (syn=VIII), SKVÐIA, (syn=SvmA), (syn= VIIIA), STvi, STK, STx1, STxn, S66, W2, (syn= D2c), (syn= D20), φl, φIVb 3-SO-R, 8368-SO-R, F7, (syn= FS7), (syn=K29), FlO, (syn=FS10), (syn=K31), I1, (syn=alpha), (syn=FSa), (syn=Kl 8), (syn=α), I2, ( syn= a), (syn= K19), SG33, (syn= G35), (syn= SO-35/G), SG35, (syn= SO-55/G), SG3201, (syn= SO-3201/ G), SHn, (syn=HII), SHv, (syn=SHV), SHx, SHX, SKn, (syn=K2), (syn=KII), (syn=Sn), (syn=SsII), ( syn=II), SKrv, (syn=Sm), (syn=SsIV), (syn=IV), SK1Va, (syn=Swab), (syn=SsIVa), (syn=IVa), SKV, (syn= K4), (syn= KV), (syn= SV), (syn= SsV), (syn= V), SKx , (syn= K9), (syn= KX), (syn= SX), (syn= SsX), (syn= X), STV, (syn= T35), (syn= 35-50-R), STvm , (syn= T8345), (syn= 8345-SO-S-R), W1, (syn= D8), (syn= FSD8), W2a, (syn= D2A), (syn= FS2a), DD-2, Sf6 , FSi, (syn= Fl), SF6, (syn= F6), SG42, (syn= SO-42/G), SG3203, (syn= SO-3203/G), SKF12, (syn= SsF12), ( syn=F12), (syn=F12), STn, (syn=1881-SO-R), γ66, (syn=gamma 66a), (syn=Ssγ66), φ2, BIl, DDVII, (syn=DD7), FSD2b, (syn= W2B), FS2, (syn= F2), (syn= F2), FS4, (syn= F4), (syn= F4), FS5, (syn= F5), (syn= F5), FS9, (syn= F9), (syn= F9), FI l, P2-S0-S, SG36, (syn= SO-36/G), (syn= G36), SG3204, (syn= SO-3204/ G), SG3244, (syn=SO-3244/G), SHi, (syn=HI), SHvπ, (syn=HVII), SHK, (syn=HIX), SHx1, SHxπ, (syn=HXn), SKI , KI, (syn=S1), (syn=SsI), SKVII, (syn=KVII), (syn=Svπ), (syn=SsVII), SKIX, (syn=KIX), (syn=S1x), ( syn=SsIX), SKXII, (syn=KXII), (syn=Sxn), (syn=SsXII), STi, STffl, STrv, STVi, STvπ, S70, S206, U2-S0-S, 3210-SO-S , 3859-SO-S, 4020-SO-S, φ3, φ5, φ7, φ8, φ9, φ10, φll, φl3, φl 4, φ18, SHm, (syn=Hπi), SHχi, (syn=HXt) and SKxI, (syn=KXI), (syn=Sχi), (syn=SsXI), (syn=XI).

Bacteria of the genus Staphylococcus can be infected by the following phages: A, EW, K, Ph5, Ph9, PhIO, Phl3, Pl, P2, P3, P4, P8, P9, PlO, RG, SB-i, (syn=Sb-I), S3K, Tweet, ΦSK311, φ812, 06, 40, 58, 119, 130, 131, 200, 1623, STCl, (syn=stcl), STC2, (syn=stc2), 44AHJD , 68, ACl, AC2, A6"C", A9"C", b581, CA-I, CA-2, CA-3, CA-4, CA-5, DIl, L39x35, L54a, M42, Nl, N2 , N3, N4, N5, N7, N8, NlO, Ni l, N12, N13, N14, N16, Ph6, Phl2, Phl4, UC-18, U4, U15, Sl, S2, S3, S4, S5, X2, Z1, φB5-2, φD, ω, 11, (syn= φll), (syn= P11-M15), 15, 28, 28A, 29, 31, 31B, 37, 42D, (syn= P42D), 44A, 48, 51, 52, 52A, (syn=P52A), 52B, 53, 55, 69, 71, (syn=P71), 71A, 72, 75, 76, 77, 79, 80, 80α, 82, 82A, 83 A, 84, 85, 86, 88, 88A, 89, 90, 92, 95, 96, 102, 107, 108, 111, 129-26, 130, 130A, 155, 157, 157A, 165, 187, 275 , 275A, 275B, 356, 456, 459, 471, 471A, 489, 581, 676, 898, 1139, 1154A, 1259, 1314, 1380, 1405, 1563, 2148, 2638A, 2638B, 2638C, 2731, 2792A, 2792B , 2818, 2835, 2848A, 3619, 5841, 12100, AC3, A8, AlO, A13, b594n, D, HK2, N9, N15, P52, P87, Sl, S6, Z4, φRE, 3A, 3B, 3C, 6, 7, 16, 21, 42B, 42C, 42E, 44, 47, 47A5 47C, 51, 54, 54x1, 70, 73, 75, 78, 81, 82 , 88, 93, 94, 101, 105, 110, 115, 129/16, 174, 594n, 1363/14, 2460 and mS-Staphylococcus (1).

Bacteria of the genus Streptococcus can be infected by the following phages: EJ-I, NN-streptococci (1), a, Cl, FL0Ths, H39, Cp-I, Cρ-5, Cp-7, Cp- 9, Cp-IO, AT298, A5, alO/Jl, alO/J2, alO/J5, alO/J9, A25, BTI l, b6, CAl, c20-l, c20-2, DP-I, Dp-4 , DTl, ET42, elO, FA101, FEThs, Fκ, FKKIOI, FKLIO, FKP74, FKH, FLOThs, FyIOl, fl, F10, F20140/76, g, GT-234, HB3, (syn=HB-3), HB -623, HB-746, M102, O1205, φO1205, PST, PO, Pl, P2, P3, P5, P6, P8, P9, P9, P12, P13, P14, P49, P50, P51, P52, P53, P54 , P55, P56, P57, P58, P59, P64, P67, P69, P71, P73, P75, P76, P77, P82, P83, P88, sc, sch, sf, SfIl 1, (syn=SFiI l), ( syn= φSFill), (syn= ΦSfil l), (syn= φSfil l), sfil9, (syn= SFil9), (syn= φSFil9), (syn= φSfil9), Sfi21, (syn= SFi21), (syn= φSFi21), (syn= φSfi21), ST0, STX, st2, ST2, ST4, S3, (syn= φS3), s265, φ17, φ42, φ57, φ80, φ81, φ82, φ83, φ84, φ85, φ86, φ87 , φ88, φ89, φ90, φ91, φ92, φ93, φ94, φ95, φ96, φ97, φ98, φ99, φ100, φlOl, φlO2, φ227, Φ7201, ωl, ω2, ω3, ω4, ω8, ω , 1, 6, 9, 1OF, 12/12, 14, 17SR, 19S, 24, 50/33, 50/34, 55/14, 55/15, 70/35, 70/36, 71/ST15, 71/45, 71/46, 74F, 79/37, 79/38, 80/J4, 80/J9, 80/ST16, 80/15, 80/47, 80/48, 101, 103/39, 103/40, 121/41, 121/42, 123/43, 123/44, 124/44, 337/ST17 and mStreptococcus (34).

Bacteria of the genus Treponema can be infected by the following phages: NN-Treponema (1).

Bacteria of the genus Vibrio can be infected by the following phages: CTXΦ, fs, (syn=si), fs2, Ivpf5, Vfl2, Vf33, VPIΦ, VSK, v6, 493, CP-Tl, ET25, kappa, K139, Labol ,) XN-69P, OXN-86, O6N-21P, PB-I, P147, rp-1, SE3, VA-I, (syn=VcA-I), VcA-2, VPl, VP2, VP4, VP7, VP8, VP9, VPlO, VP17, VP18, VP19, X29, (syn=29 derel), t, ΦHAWI-1, ΦHAWI-2, ΦHAWI-3, ΦHAWI-4, ΦHAWI-5, ΦHAWI-6, ΦHAWI-7 , XHAWI-8, ΦHAWI-9, ΦHAWI-10, ΦHCl-1, ΦHC1-2, ΦHC1-3, ΦHC1-4, ΦHC2-1, >HC2-2, ΦHC2-3, ΦHC2-4, ΦHC3-1, ΦHC3-2, ΦHC3-3, ΦHD1S-1, ΦHD1S-2, ΦHD2S-1, ΦHD2S-2, ΦHD2S-3, ΦHD2S-4, ΦHD2S-5, ΦHDO-1, ΦHDO-2, ΦHDO-3, ΦHDO- 4, ΦHDO-5, ΦHDO-6, ΦKL-33, ΦKL-34, ΦKL-35, ΦKL-36, ΦKWH-2, ΦKWH-3, ΦKWH-4, ΦMARQ-1, ΦMARQ-2, ΦMARQ-3, ΦMOAT-1, ΦO139, ΦPEL1A-1, ΦPEL1A-2, ΦPEL8A-1, ΦPEL8A-2, ΦPEL8A-3, ΦPEL8C-1, ΦPEL8C-2, ΦPEL13A-1, ΦPEL13B-1, ΦPEL13B-2, ΦPEL13B ØPEL13B-4, ØPEL13B-5, ØPEL13B-6, ØPEL13B-7, ØPEL13B-8, ØPEL13B-9, ØPEL13B-10, ØVP143, ØVP253, Ø16, Øl38, 1- II, 5, 13, 14, 16, 24, 32, 493, 6214, 7050, 7227, II, (syn=group II), (syn== φ2), V, VIII, ~m-vibrio (13), KVP20 , KVP40, nt-1, O6N-22P, P68, el, e2, e3, e4, e5, FK, G, I, K, nt-6, Nl, N2, N3, N4, N5, O6N-34P, OXN -72P, OXN-85P, OXN-100P, P, Ph-I, PL163/10, Q, S, T, φ92, 1-9, 37, 51, 57, 70A-8, 72A-4, 72A-10 , 110A-4, 333, 4996, I (syn=Group I), III (syn=Group III), VI, (syn=A-Saratov), VII, IX, X, HN-Vibrio (6), pAl , 7, 7-8, 70A-2, 71A-6, 72A-5, 72A-8, 108A-10, 109A-6, 109A-8, llOA-1, 110A-5, 110A-7, hv-1 , OXN-52P, P13, P38, P53, P65, P108, Pill, TPl3 VP3, VP6, VP12, VP13, 70A-3, 70A-4, 70A-10, 72A-1, 108A-3, 109-B1, 110A-2, 149, (syn= φ149), IV, (syn= group IV), NN-Vibrio (22), VP5, VPIl, VP15, VP16, α1, α2, α3a, α3b, 353B and HN-Vibrio ( 7).

Bacteria of the genus Yersinia can be infected by the following phages: H, H-I, H-2, H-3, H-4, Lucas 110, Lucas 303, Lucas 404, YerA3, YerA7, YerA20, YerA41, 3/M64 -76, 5/G394-76, 6/C753-76, 8/C239-76, 9/F18167, 1701, 1710, PST, 1/F2852-76, Derrell, EV, H, Kotlyarova, PTB, R , Y, YerA41, φYerO3-12, 3, 4/C1324-76, 7/F783-76, 903, 1/M6176 and Yer2AT.

In one embodiment, the bacteriophage is selected from the group consisting of Salmonella Virus SKML39, Shigella Virus AG3, Dickkey Virus Limestone, Dickkeya Virus RC2014, Escherichia Virus CBA120, Escherichia Virus PhaxI, Salmonella Virus 38 , Salmonella Virus Det7, Salmonella Virus GG32, Salmonella Virus PM10, Salmonella Virus SFP10, Salmonella Virus SH19, Salmonella Virus SJ3, Escherichia Virus ECML4, Salmonella Virus Marshall, Salmonella Virus Mayard, Salmonella Virus SJ2, Salmonella Virus STML131, Salmonella Virus STML131 ViI, Erwinia virus Ea2809, Klebsiella virus 0507KN21, Serratia virus IME250, Serratia virus MAM1, Campylobacter virus CP21, Campylobacter virus CP220, Campylobacter virus CPt10, Campylobacter virus IBB35, Campylobacter Virus CP81, Campylobacter virus CP30A, Campylobacter virus CPX, Campylobacter virus NCTC12673, Erwinia virus Ea214, Erwinia virus M7, Escherichia virus AYO145A, Escherichia virus EC6, Escherichia virus HY02, S Querichia Virus JH2, Escherichia Virus TP1, Escherichia Virus VpaE1, Escherichia Virus wV8, Salmonella Virus Felixo1, Salmonella Virus HB2014, Salmonella Virus Mushroom, Salmonella Virus UAB87, Citrobacter Virus Moogle, Citrobacter Virus modin, Escherichia virus SUSP1, Escherichia virus SUSP2, Aromonas virus phiO18P, Haemophilus virus HP1, Haemophilus virus HP2, Pasteurella virus F108, Vibrio virus K139, Vibrio virus kappa, Burkholderia virus phi52237, burkholderia virus phiE122, bur Kholderia virus phiE202, Escherichia virus 186, Escherichia virus P4, Escherichia virus P2, Escherichia virus Wphi, Manhemia virus PHL101, Pseudomonas virus phiCTX, Ralstonia virus RSA1, Salmonella virus Fels2, Salmonella Virus PsP3, Salmonella Virus SopEphi, Yersinia Virus L413C, Staphylococcus Virus G1, Staphylococcus Virus G15, Staphylococcus Virus JD7, Staphylococcus Virus K, Staphylococcus Virus MCE2014, Staphylococcus Coccus Virus P108, Staphylococcus Virus Rodi, Staphylococcus Virus S253, Staphylococcus Virus S25-4, Staphylococcus Virus SA12, Listeria Virus A511, Listeria Virus P100, Staphylococcus Virus Remus, Staphylococcus virus SA11, Staphylococcus virus Stau2, Bacillus virus camphawk, Bacillus virus SPO1, Bacillus virus BCP78, Bacillus virus charbomba, Staphylococcus virus twat, Enterococcus virus phiEC24C, Lactobacillus virus Lb338 -1, Lactobacillus virus LP65, Enterobacter virus PG7, Escherichia virus CC31, Klebsiella virus JD18, Klebsiella virus PKO111, Escherichia virus Bp7, Escherichia virus IME08, Escherichia virus JS10, S Kerichia virus JS98, Escherichia virus QL01, Escherichia virus VR5, Enterobacter virus Eap3, Klebsiella virus KP15, Klebsiella virus KP27, Klebsiella virus matis, Klebsiella virus labyrinth, Citrobacter virus Merlin, Citrobacter virus phylum, Escherichia virus JSE, Escherichia virus phi1, Escherichia virus RB49, Escherichia virus HX01, Escherichia bar Virus JS09, Escherichia Virus RB69, Shigella Virus UTAM, Salmonella Virus S16, Salmonella Virus STML198, Vibrio Virus KVP40, Vibrio Virus nt1, Vibrio Virus ValKK3, Escherichia Virus VR7, Escherichia Virus VR20, Escherichia Virus VR25, Escherichia virus VR26, Shigella virus SP18, Escherichia virus AR1, Escherichia virus C40, Escherichia virus E112, Escherichia virus ECML134, Escherichia virus HY01, Escherichia virus Ime09, S Kerichia virus RB3, Escherichia virus RB14, Escherichia virus T4, Shigella virus Pss1, Shigella virus Shfl2, Yersinia virus D1, Yersinia virus PST, Acinetobacter virus 133, Aromonas virus 65, Aeromonas Virus Aeh1, Escherichia Virus RB16, Escherichia Virus RB32, Escherichia Virus RB43, Pseudomonas Virus 42, Chronobacter Virus CR3, Chronobacter Virus CR8, Chronobacter Virus CR9, Chronobacter Virus PBES02, Pectobacterium Virus phiTE, Chronobacter Virus GAP31, Escherichia Virus 4MG, Salmonella Virus SE1, Salmonella Virus SSE121, Escherichia Virus FFH2, Escherichia Virus FV3, Escherichia Virus JES2013, Escherichia Virus V5, Brevibacillus Virus Avou Oh, Brevibacillus virus Davis, Bacillus virus agate, Bacillus virus bov, Bacillus virus Bp8pC, Erwinia virus deimos, Erwinia virus Ea35-70, Erwinia virus RAY, Erwinia virus Simmy50, Erwinia virus special G, Acineto. pylori virus AB1, acinetobacter virus AB2, acinetobacter virus AbC62, Acinetobacter Virus AP22, Atrobacter Virus ArV1, Atrobacter Virus Trina, Bacillus Virus AvesoBmore, Bacillus Virus B4, Bacillus Virus Bigversa, Bacillus Virus Lily, Bacillus Virus Spoke, Bacillus Virus Troll, Bacillus Virus Bastille, Bacillus Virus CAM003, Bacillus virus Bc431, Bacillus virus Bcp1, Bacillus virus BCP82, Bacillus virus BM15, Bacillus virus Deep Blue, Bacillus virus JBP901, Burkholderia virus Bcep1, Burkholderia virus Bcep43, Burkholderia virus Bcep781, Burkholderia virus BcepNY3, Xanthomonas virus OP2, Burkholderia virus BcepMu, Burkholderia virus phiE255, Aromonas virus 44RR2, Mycobacterium virus Alice, Mycobacterium virus Bxz1, Mycobacterium virus dandelion, Mycobacterium virus HyRo, Mycobacterium virus I3, Mycobacterium virus Nafi, Mycobacterium virus sevata, Clostridium virus phiC2, Clostridium virus phiCD27, Clostridium virus phiCD119, Bacillus virus CP51, Bacillus virus JL, Bacillus virus Shannett, Escherichia virus CVM10, Escherichia virus ep3, Erwinia virus acecino, Erwinia virus EaH2, Pseudomonas virus EL, Halomonas virus HAP1, Vibrio virus VP882, Brevibacillus virus zimmer, Brevibacillus virus Osiris , Pseudomonas virus Ab03, Pseudomonas virus KPP10, Pseudomonas virus PAKP3, Synorizobium virus M7, Synorizovium virus M12, Synorizobium virus N3, Erwinia virus machina, Atrobacter virus Brent, Atrobacter virus johnsky, Art robacter virus masha, atrobacter virus cattle Ni, Edward Siella Virus MSW3, Edward Siella Virus PEi21, Escherichia Virus Mu, Shigella Virus SfMu, Halobacterium Virus phiH, Bacillus Virus Grass, Bacillus Virus NIT1, Bacillus Virus SPG24, Aromonas Virus 43, Escherichia Chia virus P1, Pseudomonas virus CAb1, Pseudomonas virus CAb02, Pseudomonas virus JG004, Pseudomonas virus PAKP1, Pseudomonas virus PAKP4, Pseudomonas virus PaP1, Burkholderia virus BcepF1, Pseudomonas virus 141, Pseudomonas virus AbDL68, Pseudomonas virus Ab28, Pseudomonas virus Ab28, Pseudomonas virus F8, Pseudomonas virus JG024, Pseudomonas virus KPP12, Pseudomonas virus LBL3, Pseudomonas virus LMA2, Pseudomonas virus PB1, Pseudomonas virus SN, Pseudomonas virus PA7, Pseudomonas virus phiKZ, Rhizobium virus RHEph4, Pseudomonas virus RHE Virus RSL2, Ralstonia virus RSL1, Aromonas virus 25, Aromonas virus 31, Aromonas virus Aes12, Aromonas virus Aes508, Aromonas virus AS4, Stenotrophomonas virus IME13, Staphylococcus virus IPLAC1C, Star Philococcus virus SEP1, Salmonella virus SPN3US, Bacillus virus 1, Geobacillus virus GBSV1, Yersinia virus R1RT, Yersinia virus TG1, Bacillus virus G, Bacillus virus PBS1, Microcystis virus Ma-LMM01, Vibrio virus MAR, Vibrio Virus VHML, Vibrio Virus VP585, Bacillus Virus BPS13, Bacillus Virus Hakuna, Bacillus Virus Megatron, Bacillus Virus WPh, Acinetobacter Virus AB3, Acinetobacter Virus Escherichia virus Abp1, Acinetobacter virus Fri1, Acinetobacter virus IME200, Acinetobacter virus PD6A3, Acinetobacter virus PDAB9, Acinetobacter virus phiAB1, Escherichia virus K30, Klebsiella virus K5, Klebsiella virus K11 , Klebsiella virus Kp1, Klebsiella virus KP32, Klebsiella virus KpV289, Klebsiella virus F19, Klebsiella virus K244, Klebsiella virus Kp2, Klebsiella virus KP34, Klebsiella virus KpV41, Kleb Siella virus KpV71, Klebsiella virus KpV475, Klebsiella virus SU503, Klebsiella virus SU552A, Pantoea virus Limelight, Pantoea virus Lymezero, Pseudomonas virus LKA1, Pseudomonas virus phiKMV, Xanthomonas virus f20, Xanthomonas virus f30, Xylella virus Prado, Erwinia virus Era103, Escherichia virus K5, Escherichia virus K1-5, Escherichia virus K1E, Salmonella virus SP6, Escherichia virus T7, Kluyvera virus Kvp1, Pseudomonas virus gh1, prochlorococcus virus PSSP7, synecococcus virus P60, synecococcus virus Syn5, streptococcus virus Cp1, streptococcus virus Cp7, staphylococcal virus 44AHJD, streptococcus virus C1, bacillus virus B103, Bacillus virus GA1, Bacillus virus phi29, Curtia virus 6, Actinomyces virus Av1, Mycoplasma virus P1, Escherichia virus 24B, Escherichia virus 933W, Escherichia virus Min27, Escherichia virus PA28 , Escherichia Virus Stx2 II, Shigella Virus 7502Stx, Shigella Virus POCJ13, Escherichia Virus 191, Escherichia Virus PA2, Escherichia virus TL2011, Shigella virus VASD, Burkholderia virus Bcep22, Burkholderia virus Bcepil02, Burkholderia virus Bcepmigl, Burkholderia virus DC1, Bordetella virus BPP1, Burkholderia virus BcepC6B, Cellulopa Viral virus Cba41, Cellulopagavirus Cba172, Dinoroseobacter virus DFL12, Erwinia virus Ea9-2, Erwinia virus frozen, Escherichia virus phiV10, Salmonella virus epsilon15, Salmonella virus SPN1S, Pseudomonas virus F116, Pseudomonas Virus H66, Escherichia virus APEC5, Escherichia virus APEC7, Escherichia virus Bp4, Escherichia virus EC1UPM, Escherichia virus ECBP1, Escherichia virus G7C, Escherichia virus IME11, Shigella virus Sb1, Acromobacter virus Axp3, Acromobacter virus JWAlpha, Edward Siella virus KF1, Pseudomonas virus KPP25, Pseudomonas virus R18, Pseudomonas virus Ab09, Pseudomonas virus LIT1, Pseudomonas virus PA26, Pseudomonas virus Ab22, Pseudomonas virus CHU, Pseudomonas virus CHU Pseudomonas virus PAA2, Pseudomonas virus PaP3, Pseudomonas virus PaP4, Pseudomonas virus TL, Pseudomonas virus KPP21, Pseudomonas virus LUZ7, Escherichia virus N4, Salmonella virus 9NA, Salmonella virus SP069, Salmonella virus P22, Salmonella virus BTP1, Salmonella virus BTP1, Salmonella Virus ST64T, Shigella Virus Sf6, Bacillus Virus Page, Bacillus Virus Palmer, Bacillus Virus Pascal, Bacillus Virus Pony, Bacillus Virus Puki, Escherichia Virus 172-1, Escherichia Virus EC B2, Escherichia Virus NJ01, Escherichia Virus phiEco32, Escherichia Virus Septima11, Escherichia Virus SU10, Brucella Virus Pr, Brucella Virus Tb, Escherichia Virus Pollock, Salmonella Virus FSL SP-058, Salmonella Virus FSL SP-076, Helicobacter virus 1961P, Helicobacter virus KHP30, Helicobacter virus KHP40, Hamiltonella virus APSE1, Lactococcus virus KSY1, Formidium virus WMP3, Formidium virus WMP4, Pseudomonas virus 119X, Roseobacter virus SIO1, Vibrio Virus VpV262, Vibrio Virus VC8, Vibrio Virus VP2, Vibrio Virus VP5, Streptomyces Virus Amella, Streptomyces Virus phiCAM, Streptomyces Virus Aronoculus, Streptomyces Virus Caliburn, Streptomyces Virus Complexna , Streptomyces Virus Hydra, Streptomyces Virus Idge, Streptomyces Virus Lannister, Streptomyces Virus Rica, Streptomyces Virus Susidade, Streptomyces Virus Zemlya, Streptomyces Virus ELB20, Streptomyces Virus R4, Streptomyces virus phiHau3, Mycobacterium virus Arcadian, Mycobacterium virus Bae, Mycobacterium virus reprovate, Mycobacterium virus adawi, Mycobacterium virus Bane1, Mycobacterium Therium Virus Brown CNA, Mycobacterium Virus Krismich, Mycobacterium Virus Cooper, Mycobacterium Virus Jamal, Mycobacterium Virus Nigel, Mycobacterium Virus Stinger, Mycobacterium Virus Vincenzo, Mycobacterium Virus cobacterium virus gemanar, mycobacterium virus apigeum, mycobacterium virus manad, mycobacterium virus olin, mycobacterium virus osmaximus, mycobacterium Virus Pg1, Mycobacterium Virus Soto, Mycobacterium Virus Suffolk, Mycobacterium Virus Athena, Mycobacterium Virus Bernardo, Mycobacterium Virus Gadget, Mycobacterium Virus Pipefish, Mycobacterium Virus Godins, Mycobacterium Virus Rosebush, Mycobacterium Virus Babsiella, Mycobacterium Virus Bruchta, Mycobacterium Virus Che9c, Mycobacterium Virus Sbash, Mycobacterium Virus Hawkeye, Mycobacterium virus float, Salmonella virus AG11, Salmonella virus Ent1, Salmonella virus f18SE, Salmonella virus arrest, Salmonella virus L13, Salmonella virus LSPA1, Salmonella virus SE2, Salmonella virus SETP3, Salmonella virus SETP7, Salmonella virus SETP13, Salmonella virus SP101 , Salmonella virus SS3e, Salmonella virus wksl3, Escherichia virus K1G, Escherichia virus K1H, Escherichia virus K1ind1, Escherichia virus K1ind2, Salmonella virus SP31, Leukonostok virus Lmd1, Leukonostok virus LN Konostock virus LN04, leukonostock virus LN12, leukonostock virus LN6B, leukonostock virus P793, leukonostock virus 1A4, leukonostock virus Ln8, leukonostock virus Ln9, leukonostock virus LN25, leukonostock Virus LN34, Leukonostok Virus LNTR3, Mycobacterium Virus Bongo, Mycobacterium Virus Ray, Mycobacterium Virus Butters, Mycobacterium Virus Micelle, Mycobacterium Virus Charlie, Mycobacterium Virus phipsquix, mycobacterium virus xeno, mycobacterium virus pancino, mycobacterium virus fran, mycobacterium virus ready, mycobacterium virus skinnip, Gordonia virus Baxterfox, Gordonia virus easy , Gordonia virus kita, Gordonia virus zirinka, Gordonia virus nympadora, Mycobacterium virus big noose, Mycobacterium virus brusacoram, Mycobacterium virus donovan, Mycobacterium virus fishbone, Mycobacterium virus zevex, mycobacterium virus mality, mycobacterium virus pions, Enterobacter virus F20, Klebsiella virus 1513, Klebsiella virus KLPN1, Klebsiella virus KP36, Klebsiella Virus PKP126, Klebsiella Virus Susi, Escherichia Virus AHP42, Escherichia Virus AHS24, Escherichia Virus AKS96, Escherichia Virus C119, Escherichia Virus E41c, Escherichia Virus Eb49, Escherichia Virus Jk06 , Escherichia virus KP26, Escherichia virus Rogue1, Escherichia virus ACGM12, Escherichia virus Rtp, Escherichia virus ADB2, Escherichia virus JMPW1, Escherichia virus JMPW2, Escherichia virus T1, Shigel Ravirus PSf2, Shigella Virus Shfl1, Citrobacter Virus Stevie, Escherichia Virus TLS, Salmonella Virus SP126, Chronobacter Virus Esp2949-1, Pseudomonas Virus Ab18, Pseudomonas Virus Ab19, Pseudomonas Virus PaMx11, Atrobacter Virus Amigo, Propionibacterium Virus Anatole, Propionibacterium Virus B3, Bacillus Virus Andromeda, Bacillus Virus Blastoid, Bacillus Virus Curly, Bacillus Virus Owen, Bacillus Virus Pin, Bacillus Virus Glittering, Bacillus Virus Rigi, Bacillus Virus Taylor, Gor donia virus artis, mycobacterium virus banyad, mycobacterium virus constantin, mycobacterium virus predator, mycobacterium virus Bernal13, staphylococcus virus 13, Staphylococcus Virus 77, Staphylococcus Virus 108PVL, Mycobacterium Virus Bronn, Mycobacterium Virus Faith1, Mycobacterium Virus Jodert, Mycobacterium Virus Rumpelstilskin, Lactococcus Virus bIL67 , Lactococcus virus c2, Lactobacillus virus c5, Lactobacillus virus Ld3, Lactobacillus virus Ld17, Lactobacillus virus Ld25A, Lactobacillus virus LLKu, Lactobacillus virus phiLdb, Cellulopaga virus Cba121, Cellulopaga virus Cba171 , Cellulopaga Virus Cba181, Cellulopaga Virus ST, Bacillus Virus 250, Bacillus Virus IEBH, Mycobacterium Virus Admore, Mycobacterium Virus Avani, Mycobacterium Virus Boomer, Mycobacterium Virus Che8, Mycobacterium Virus Che9d, Mycobacterium Virus Deadp, Mycobacterium Virus Dran, Mycobacterium Virus Dorothy, Mycobacterium Virus Dot Product, Mycobacterium Virus Drago, Mycobacterium Virus Fruit Loop, Mycobacterium Virus Gumbi, Mycobacterium Virus Ibubesi, Mycobacterium Virus Llij, Mycobacterium Virus Moji, Mycobacterium Virus Mutaforma 13, Mycobacterium Virus Pacc40, Mycobacterium virus PMC, Mycobacterium virus Ramsay, Mycobacterium virus rocky horror, Mycobacterium virus SG4, Mycobacterium virus Shauna 1, Mycobacterium virus silane, Mycobacterium Virus spartacus, mycobacterium virus taj, mycobacterium virus tweety, mycobacterium virus wii, mycobacterium virus yoshi, salmonella virus chi, salmonella virus FSLSP030, salmonella virus FSLSP088, salmonella virus iEPS5, salmonella virus SPN19 , Mycobacterium Virus 244, Mycobacterium Virus Bas k21, mycobacterium virus CJW1, mycobacterium virus eureka, mycobacterium virus costia, mycobacterium virus pocky, mycobacterium virus pumpkin, mycobacterium virus siduracell, mycobacterium Rium virus toto, Mycobacterium virus condog, Mycobacterium virus firecracker, Rhodobacter virus RcCronus, Pseudomonas virus D3112, Pseudomonas virus DMS3, Pseudomonas virus FHA0480, Pseudomonas virus LPB1, Pseudomonas virus MP22, Pseudomonas virus MP29, Pseudomonas virus MP29 Virus MP38, Pseudomonas Virus PA1KOR, Pseudomonas Virus D3, Pseudomonas Virus PMG1, Atrobacter Virus Decuro, Gordonia Virus Demosthenes, Gordonia Virus Katyusha, Gordonia Virus Kbote, Propionibacterium Virus B22, Propionibacterium Virus Two sets, Propionibacterium virus E6, Propionibacterium virus G4, Burkholderia virus phi6442, Burkholderia virus phi1026b, Burkholderia virus phiE125, Edward Siella virus eiAU, Mycobacterium virus Ff47, Mycobacterium Therium Virus Moody, Mycobacterium Virus Gaia, Mycobacterium Virus Giles, Atrobacter Virus Captain Murica, Atrobacter Virus Gordon, Gordonia Virus GordTnk2, Paengnibacillus Virus Harrison, Escherichia Virus EK99P1, Escherichia virus HK578, Escherichia virus JL1, Escherichia virus SSL2009a, Escherichia virus YD2008s, Shigella virus EP23, Sodalis virus SO1, Escherichia virus HK022, Escherichia virus HK75, Escherichia virus HK97, Escherichia virus HK106, Escherichia virus HK446, Escherichia virus HK542, Escherichia virus HK544, Escherichia virus Lycia virus HK633, Escherichia virus mEp234, Escherichia virus mEp235, Escherichia virus mEpX1, Escherichia virus mEpX2, Escherichia virus mEp043, Escherichia virus mEp213, Escherichia virus mEp237 Virus mEp390, Escherichia Virus mEp460, Escherichia Virus mEp505, Escherichia Virus mEp506, Brevibacillus Virus Genst, Acromobacter Virus 83-24, Acromobacter Virus JWX, Atrobacter Virus Kellegio, Art Atrobacter Virus KitKat, Atrobacter Virus Benny, Atrobacter Virus DrRobert, Atrobacter Virus Glenn, Atrobacter Virus Hunterdal, Atrobacter Virus Joan, Atrobacter Virus Cora, Atrobacter Virus Preamble, Art robacter virus fumancara, atrobacter virus wayne, mycobacterium virus alma, mycobacterium virus arturo, mycobacterium virus astro, mycobacterium virus bag yard digan, mycobacterium virus BBPiebs31, my cobacterium virus benedict, mycobacterium virus bethlehem, mycobacterium virus vilnuckles, mycobacterium virus Bruns, mycobacterium virus Bxb1, mycobacterium virus Bxz2, mycobacterium virus Che12, Mycobacterium virus Cuco, Mycobacterium virus D29, Mycobacterium virus doom, Mycobacterium virus Ericb, Mycobacterium virus euphoria, Mycobacterium virus George, Mycobacterium virus gladiator, Mycobacterium virus goose, mycobacterium virus hammer, mycobacterium virus heldan, mycobacterium virus jasper, mycobacterium virus JC27, mycobacterium virus Jeffavani, mycobacterium virus JHC117, Mycobacterium virus KBG, Mycobacterium virus Kssjeb, Mycobacterium Virus Kugel, Mycobacterium Virus L5, Mycobacterium Virus Resedi, Mycobacterium Virus LHTSCC, Mycobacterium Virus Rockley, Mycobacterium Virus Marcel, Mycobacterium Virus Microwolf, Mycobacterium Therium Virus Mrgordo, Mycobacterium Virus Museum, Mycobacterium Virus Nepal, Mycobacterium Virus Pacman, Mycobacterium Virus Peaches, Mycobacterium Virus Perseus, Mycobacterium Virus Fukovnik, Mycobacterium Virus Rebeuka, Mycobacterium Virus Red Rock, Mycobacterium Virus Ridgecb, Mycobacterium Virus Rockstar, Mycobacterium Virus Saintus, Mycobacterium Virus Schiphol, Mycobacterium Virus Solon, Mycobacterium Virus Switcher, Mycobacterium Virus SWU1, Mycobacterium Virus Ta17a, Mycobacterium Virus Tiger, Mycobacterium Virus Team Cell, Mycobacterium Virus Tricie, Mycobacterium Virus turbido, Mycobacterium virus twister, Mycobacterium virus U2, Mycobacterium virus violet, Mycobacterium virus wonder, Escherichia virus DE3, Escherichia virus HK629, Escherichia virus HK630, Escherichia virus lambda, Atrobacter virus raroye, mycobacterium virus halo, mycobacterium virus liphi, mycobacterium virus marvin, mycobacterium virus MOSmoris, Atrobacter virus circum, atro Bacterivirus Mudcat, Escherichia Virus N15, Escherichia Virus 9g, Escherichia Virus JenK1, Escherichia Virus JenP1, Escherichia Virus JenP2, Pseudomonas Virus NP1, Pseudomonas Virus PaMx25, Mycobacterium Virus Bacca, Mycobacterium Virus Courthouse, Mycobacterium Virus Little, Mycobacterium Virus Omega, Mycobacterium Virus Optimus, Mycobacterium Leum virus Tybalt, Polaribacter virus P12002L, Polaribacter virus P12002S, Non-Ravens virus P12024L, Non-Ravens virus P12024S, Thermus virus P23-45, Thermus virus P74-26, Listeria virus LP26, Listeria virus LP37, Listeria virus LP110, Listeria virus LP114, Listeria virus P70, Propionibacterium virus ATCC29399BC, Propionibacterium virus ATCC29399BT, Propionibacterium virus Attacne, Propionibacterium virus Keiki, Propionibacterium virus Kubed, Propionibacterium virus Lauchelly, Propionibacterium virus MrAK, Propionibacterium virus Ouroborose, Propionibacterium virus P91, Propionibacterium virus P105, Propionibacterium virus P144, Propionibacterium virus P1001, Propionibacterium Virus P1.1, Propionibacterium virus P100A, Propionibacterium virus P100D, Propionibacterium virus P101A, Propionibacterium virus P104A, Propionibacterium virus PA6, Propionibacterium virus P acnes 201215, Propionibacterium virus PAD20, Propionibacterium virus PAS50, Propionibacterium virus PHL009M11, Propionibacterium virus PHL025M00, Propionibacterium virus PHL037M02, Propionibacterium virus PHL041M10, Propionibacterium virus PHL060L00, Propionibacterium virus PHL060L00 Onibacterium virus PHL067M01, Propionibacterium virus PHL070N00, Propionibacterium virus PHL071N05, Propionibacterium virus PHL082M03, Propionibacterium virus PHL092M00, Propionibacterium virus PHL095N00, Propionibacterium virus PHL111M01 , Propionibacterium virus PHL112N00, Propionibacterium virus PHL113M01, Propionibacterium virus PHL114L00, Propionibacterium virus PHL116M00, Propionibacterium virus PHL117M00, Propionibacterium virus PHL117M01, Propionibacterium virus PHL132N00, Propionibacterium virus PHL141N00, Propionibacterium virus PHL151M00, Propionibacterium virus PHL151N00, Propionibacterium virus PHL152M00, Propionibacterium virus PHL163M00, Propionibacterium virus PHL171M01, Propionibacterium virus PHL179M00, Propionibacterium virus PHL179M00 Onibacterium virus PHL194M00, Propionibacterium virus PHL199M00, Propionibacterium virus PHL301M00, Propionibacterium virus PHL308M00, Propionibacterium virus Pirate, Propionibacterium virus Procras 1, Propionibacterium virus SKKY, Propionibacterium virus solid, Propionibacterium virus stormbone, Propionibacterium virus Wizzo, Pseudomonas virus PaMx28, Pseudomonas virus PaMx74, Mycobacterium virus patences, Mycobacterium virus PBI1, Rhodococcus virus Pepy6, Rhodococcus virus Poco6, Propionibacterium virus PFR1, Streptomyces virus phiBT1, Streptomyces virus phiC31, Streptomyces virus TG1, Caulobacter virus karma, Caulobacter virus magneto, Caulobacter virus phiCbK, Caul robacter virus log, cowlobacter virus swift, staphylococcus virus 11, staphylococcus virus 29, staphylococcus virus 37, staphylococcus virus 53, staphylococcus virus 55, staphylococcus Virus 69, Staphylococcus Virus 71, Staphylococcus cous virus 80, staphylococcus virus 85, staphylococcus virus 88, staphylococcus virus 92, staphylococcus virus 96, staphylococcus virus 187, staphylococcus virus 52a, staphylococcus Virus 80 Alpha, Staphylococcus Virus CNPH82, Staphylococcus Virus EW, Staphylococcus Virus IPLA5, Staphylococcus Virus IPLA7, Staphylococcus Virus IPLA88, Staphylococcus Virus PH15, Staphylococcus Virus phiETA, Staphylococcus Virus phiETA2, Staphylococcus Virus phiETA3, Staphylococcus Virus phiMR11, Staphylococcus Virus phiMR25, Staphylococcus Virus phiNM1, Staphylococcus Virus phiNM2, Staphylococcus Virus phiNM4, Staphylococcus Virus SAP26, Staphylococcus Virus X2, Enterococcus Virus FL1, Enterococcus Virus FL2, Enterococcus Virus FL3, Lactobacillus Virus ATCC8014, Lactobacillus Virus phiJL1, Pediococcus Virus cIP1, Aromonas virus pIS4A, Listeria virus LP302, Listeria virus PSA, Methanobacterium virus psiM1, Roseobacter virus RDJL1, Roseobacter virus RDJL2, Rhodococcus virus RER2, Enterococcus virus BC611, Enterococcus virus IMEEF1 Enterococcus virus SAP6, Enterococcus virus VD13, Streptococcus virus SPQS1, Mycobacterium virus papyrus, Mycobacterium virus Send513, Burkholderia virus KL1, Pseudomonas virus 73, Pseudomonas virus Ab26, Pseudomonas virus kahetti 25, escherichia virus caisen, escherichia virus seurat, staphylococcus virus SEP9, staphylococcus virus sextag, streptococcus virus 858, streptococcus virus S 2972, Streptococcus virus ALQ132, Streptococcus virus O1205, Streptococcus virus Sfi11, Streptococcus virus 7201, Streptococcus virus DT1, Streptococcus virus phiAbc2, Streptococcus virus Sfi19, Streptococcus virus Sfi21 , paenibacillus virus diva, paenibacillus virus Hb10c2, paenibacillus virus rani, paenibacillus virus celli, paenibacillus virus cytara, paenibacillus virus willow, lactococcus virus 712, lactococcus virus ASCC191, Lactococcus Virus ASCC273, Lactococcus Virus ASCC281, Lactococcus Virus ASCC465, Lactococcus Virus ASCC532, Lactococcus Virus Bibb29, Lactococcus Virus bIL170, Lactococcus Virus CB13, Lactococcus Virus CB14, Lactococcus Virus Cous Virus CB19, Lactococcus Virus CB20, Lactococcus Virus jj50, Lactococcus Virus P2, Lactococcus Virus P008, Lactococcus Virus sk1, Lactococcus Virus Sl4, Bacillus Virus Slash, Bacillus Virus Stahl, Bacillus Virus Staley, Bacillus Virus Stills, Gordonia Virus Vaquita, Gordonia Virus ClubL, Gordonia Virus One Work, Gordonia Virus Smoothie, Gordonia Virus Soup, Bacillus Virus SPbeta, Vibrio Virus MAR10, Vibrio Virus SSP002, Eschery Tooth virus AKFV33, Escherichia virus BF23, Escherichia virus DT57C, Escherichia virus EPS7, Escherichia virus FFH1, Escherichia virus H8, Escherichia virus slur09, Escherichia virus T5, Salmonella virus 118970sal2, Salmonella Virus Sivani, Salmonella Virus SPC35, Salmonella Virus Stitch, Atrobacter Virus Tang Chrysanthemum, tsukamurella virus TIN2, tsukamurella virus TIN3, tsukamurella virus TIN4, rhodobacter virus RcSpartan, rhodobacter virus RcTitan, mycobacterium virus anaya, mycobacterium virus angelica, mycobacterium virus Crimd , mycobacterium virus pionbath, mycobacterium virus jaws, mycobacterium virus lava, mycobacterium virus mac and cheese, mycobacterium virus pixie, mycobacterium virus TM4, bacillus virus BMBtp2, bacillus Virus TP21, Geobacillus virus Tp84, Staphylococcus virus 47, Staphylococcus virus 3a, Staphylococcus virus 42e, Staphylococcus virus IPLA35, Staphylococcus virus phi12, Staphylococcus virus phiSLT, Mycobacterium Virus 32HC, Rhodococcus Virus RGL3, Paenibacillus Virus Vegas, Gordonia Virus Vendetta, Bacillus Virus Wbeta, Mycobacterium Virus Wildcat, Gordonia Virus Twister6, Gordonia Virus Wizard, Gordonia Viruses Hotorovo, Gordonia virus monti, Gordonia virusus, Xanthomonas virus CP1, Xanthomonas virus OP1, Xanthomonas virus phil7, Xanthomonas virus Xop411, Xanthomonas virus Xp10, Streptomyces virus TP1604, Streptomyces virus YDN12 , Alpha proteobacterial virus phiJl001, Pseudomonas virus LKO4, Pseudomonas virus M6, Pseudomonas virus MP1412, Pseudomonas virus PAE1, Pseudomonas virus infant, Pseudomonas virus PM2, Pseudomonas virus phi6, Pseudomonas virus phi8, Pseudomonas virus phi13 , Pseudomonas virus phi2954, Pseudomonas virus phiNN, Pseudomonas virus phiYY, Vibrio virus Escherichia virus VGJ, Ralstonia virus RS603, Ralstonia virus RSM1, Ralstonia virus RSM3, Escherichia virus M13, Escherichia virus I22, Salmonella virus IKe, Acoleplasma virus L51, Vibrio virus fs2 , Vibrio virus VFJ, Escherichia virus If1, Propionibacterium virus B5, Pseudomonas virus Pf1, Pseudomonas virus Pf3, Ralstonia virus PE226, Ralstonia virus RSS1, Spiroplasma virus SVTS2, Stenotrophomonas virus PSH1 , Stenotrophomonas virus SMA6, Stenotrophomonas virus SMA7, Stenotrophomonas virus SMA9, Vibrio virus CTXphi, Vibrio virus KSF1, Vibrio virus VCY, Vibrio virus Vf33, Vibrio virus VfO3K6, Xanthomonas virus Cf1c, Spiroplasma virus C74, Spiroplasma virus R8A2B, Spiroplasma virus SkV1CR23x, Escherichia virus FI, Escherichia virus Qbeta, Escherichia virus BZ13, Escherichia virus MS2, Escherichia virus alpha3, Escherichia Virus ID21, Escherichia virus ID32, Escherichia virus ID62, Escherichia virus NC28, Escherichia virus NC29, Escherichia virus NC35, Escherichia virus phiK, Escherichia virus St1, Escherichia virus WA45 , Escherichia virus G4, Escherichia virus ID52, Escherichia virus alopecia, Escherichia virus phiX174, Bdelobibrio virus MAC1, Bdelobbibrio virus MH2K, Chlamydia virus Chp1, Chlamydia virus Chp2, Chlamydia virus CPAR39, Chlamydia Virus CPG1, Spiroplasma Virus SpV4, Acoleplasma Virus L2 , Pseudomonas virus PR4, Pseudomonas virus PRD1, Bacillus virus AP50, Bacillus virus Bam35, Bacillus virus GIL16, Bacillus virus Wip1, Escherichia virus phi80, Escherichia virus RB42, Escherichia virus T2, Escherichia virus T3, S Querichia Virus T6, Escherichia Virus VT2-Sa, Escherichia Virus VT1-Sacai, Escherichia Virus VT2-Sakai, Escherichia Virus CP-933V, Escherichia Virus P27, Escherichia Virus Stx2phi-I , Escherichia virus Stx1phi, Escherichia virus Stx2phi-II, Escherichia virus CP-1639, Escherichia virus BP-4795 based, Escherichia virus 86, Escherichia virus Min27, Escherichia virus 2851, Escherichia Virus 1717, Escherichia Virus YYZ-2008, Escherichia Virus EC026_P06, Escherichia Virus ECO103_P15, Escherichia Virus ECO103_P12, Escherichia Virus ECO111_P16, Escherichia Virus ECO111_P11, Escherichia Virus VT2phi Escherichia virus TL-2011c, Escherichia virus P13374, Escherichia virus Sp5.

In one embodiment, bacterial viral particles typically E. Targets E. coli and comprises a capsid of a bacteriophage selected from the group consisting of: BW73, B278, D6, D108, E, El, E24, E41, FI-2, FI-4, FI-5, HI8A, Ffl8B, i, MM, Mu, 025, PhI-5, Pk, PSP3, Pl, PlD, P2, P4, Sl, Wφ, φK13, φl, φ2, φ7, φ92, 7 A, 8φ, 9φ, 18, 28 -1, 186, 299, HH-Escherichia (2), AB48, CM, C4, C16, DD-VI, E4, E7, E28, FIl, FI3, H, Hl, H3, H8, K3, M, N, ND-2, ND-3, ND4, ND-5, ND6, ND-7, Ox-I, Ox-2, Ox-3, Ox-4, Ox-5, Ox-6, PhI-I, RB42, RB43, RB49, RB69, S, SaI-I, Sal-2, Sal-3, Sal-4, Sal-5, Sal-6, TC23, TC45, TuII*-6, TuIP-24, TuII*46 , TuIP-60, T2, T4, T6, T35, αl, 1, IA, 3, 3A, 3T+, 5φ, 9266Q, CFO103, HK620, J, K, KlF, m59, no. A, no. E, no. 3, no. 9, N4, sd, T3, T7, WPK, W31, ΔH, φC3888, φK3, φK7, φK12, φV-1, Φ04-CF, Φ05, Φ06, Φ07, φl, φl.2, φ20, φ95, φ263, φlO92, φl, φll, Ω8, 1, 3, 7, 8, 26, 27, 28-2, 29, 30, 31, 32, 38, 39, 42, 933W, NN-Escherichia (1), Esc -7-11, AC30, CVX-5, Cl, DDUP, ECl, EC2, E21, E29, Fl, F26S, F27S, Hi, HK022, HK97, HK139, HK253, HK256, K7, ND-I, PA-2 , q, S2, Tl,), T3C, T5, UC-I, w, β4, γ2, λ, ΦD326, φγ, Φ06, Φ7, Φ10, φ80, χ, 2, 4, 4A, 6, 8A, 102 , 150, 168, 174, 3000, AC6, AC7, AC28, AC43, AC50, AC57, AC81, AC95, HK243, KlO, ZG/3A, 5, 5A, 21EL, H19-J and 933H.

The present disclosure provides a pharmaceutical or veterinary composition comprising one or more bacterial delivery vehicles disclosed herein and a pharmaceutically acceptable carrier. Generally, for pharmaceutical use, the bacterial delivery vehicle is a pharmaceutical formulation comprising at least one bacterial delivery vehicle and at least one pharmaceutically acceptable carrier, diluent or excipient, and optionally one or more further pharmaceutically active compounds. or as a composition. Such formulations may be in a form suitable for oral administration, parenteral administration (eg, intravenous, intramuscular, or subcutaneous injection or intravenous infusion), topical administration, administration by inhalation, skin patches, implants, suppositories, and the like. Such dosage forms may be solid, semi-solid, or liquid, depending on the mode and route of administration. For example, formulations for oral administration may be provided with an enteric coating that permits the synthetic bacterial delivery vehicle in the formulation to withstand the gastric environment and pass into the intestine. More generally, synthetic bacterial delivery vehicle formulations for oral administration may be suitably formulated for delivery to any desired portion of the gastrointestinal tract. In addition, suitable suppositories may be used for delivery to the gastrointestinal tract. A variety of pharmaceutically acceptable carriers, diluents and excipients useful in bacterial delivery vehicle compositions are known to those of skill in the art.

Also provided is a method of treating a disease or disorder caused by a bacterium, such as a bacterial infection, using the bacterial delivery vehicle or composition disclosed herein. The method comprises administering to a subject having a bacterial infection in need thereof a bacterial delivery vehicle or composition disclosed herein. In certain embodiments, the subject is a mammal. In certain embodiments, the subject is a human.

The pharmaceutical or veterinary composition according to the present disclosure may further comprise a pharmaceutically acceptable vehicle. Solid pharmaceutically acceptable vehicles are those that can also act as flavoring agents, lubricants, solubilizers, suspending agents, dyes, fillers, glidants, compression aids, inert binders, sweeteners, preservatives, dyes, coatings, or tablet-disintegrating agents. It may include one or more substances. Suitable solid vehicles include, for example, calcium phosphate, magnesium stearate, talc, sugars, lactose, dextrin, starch, gelatin, cellulose, polyvinylpyrrolidone, low melting waxes and ion exchange resins.

Pharmaceutical or veterinary compositions may be prepared as sterile solid compositions that may be suspended upon administration using sterile water, saline, or other suitable sterile injectable medium. The pharmaceutical or veterinary compositions of the present disclosure may contain other solutes or suspending agents (eg, saline or glucose sufficient to render the solution isotonic), bile salts, acacia, gelatin, sorbitan monoleate, polysorbate 80 (sorbitol) It can be administered orally in the form of a sterile solution or suspension containing an oleate ester and anhydride thereof copolymerized with ethylene oxide). Particles according to the present disclosure may also be administered orally in the form of liquid or solid compositions. Compositions suitable for oral administration include solid forms such as pills, capsules, granules, tablets, and powders, and liquid forms such as solutions, syrups, elixirs, and suspensions. Forms useful for enteral administration include sterile solutions, emulsions, and suspensions.

Bacterial delivery vehicles according to the present disclosure may be dissolved or suspended in a pharmaceutically acceptable liquid vehicle such as water, an organic solvent, a mixture of both, or a pharmaceutically acceptable oil or fat. The liquid vehicle may contain other suitable pharmaceutical additives such as solubilizing agents, emulsifying agents, buffering agents, preservatives, sweetening agents, flavoring agents, suspending agents, thickening agents, coloring agents, viscosity modifying agents, stabilizing agents or osmotic-modifying agents. Suitable examples of liquid vehicles for oral and enteral administration include water (containing in part a solution of such additives, e.g., cellulose derivatives, preferably sodium carboxymethyl cellulose), alcohols (monohydric and polyhydric alcohols, such as glycols) and derivatives thereof, and oils (eg, fractionated coconut oil and peanut oil). For parenteral administration, the vehicle may also be an oily ester such as ethyl oleate and isopropyl myristate. Sterile liquid vehicles are useful in sterile liquid form compositions for enteral administration. The liquid vehicle for the pressurized composition may be a halogenated hydrocarbon or other pharmaceutically acceptable propellant.

For transdermal administration, the pharmaceutical or veterinary composition may be formulated in the form of an ointment, cream, or gel, and an appropriate penetrating agent or detergent such as dimethyl sulfoxide, dimethyl acetamide, and dimethylformamide may be used to facilitate penetration. have

For transmucosal administration, nasal nebulizers, rectal or vaginal suppositories may be used. The active compound may be incorporated into any known suppository base via methods known in the art. Examples of such bases include cocoa butter, polyethylene glycol (carbowax), polyethylene sorbitan monostearate, and mixtures thereof with other compatible substances to modify the melting point or dissolution rate.

Diseases or conditions caused by bacteria include abdominal cramps, acne, acute epiglottitis, arthritis, bacteremia, bloody diarrhea, botulism, brucellosis, brain abscess, gonadotropin, chlamydia, clonal disease, conjunctivitis, cholecystitis, colorectal cancer, polyps Inflammation, colonic disintegration, Lyme disease, diarrhea, diphtheria, duodenal ulcer, endocarditis, erysipelothricosis, typhoid fever, fever, glomerulonephritis, gastroenteritis, gastric ulcer, Guillain-Barré syndrome, tetanus, gonorrhea, gingivitis, inflammatory Bowel disease, irritable bowel syndrome, leptospirosis, leprosy, listeriosis, tuberculosis, lady withermere syndrome, veterans disease, meningitis, mucopurulent conjunctivitis, multidrug-resistant bacterial infection, multidrug-resistant bacterial carrier, myonecrosis-gas gangrene, mycobacteria Mycobacterium avium complex, neonatal necrotizing enterocolitis, nocardiasis, nosocomial infection, otitis, periodontitis, pharyngitis, pneumonia, peritonitis, purpura, Rocky Mountain erythematosus, shigelosis, syphilis, sinusitis, stomatitis, It may be selected from the group consisting of sepsis, subcutaneous abscess, wild rabbit disease, bronchitis, tonsillitis, typhoid, ulcerative colitis, urinary tract infection, pertussis.

Diseases or disorders caused by bacteria include skin infections such as acne, intestinal infections such as esophagitis, gastritis, enteritis, colitis, stomatitis, proctitis, and peritonitis, urinary tract infections, vaginal infections, female upper genital infections such as salpingitis, endometritis, Ovarian, hysteromyositis, paroxysmal and infection of the pelvic peritoneum, respiratory infections such as pneumonia, intramniotic infections, gingival infections, endodontic infections, fibrosis, meningitis, bloodstream infections, nosocomial infections such as catheter-related infections, hospital acquired pneumonia, postpartum infection, hospital acquired gastroenteritis, hospital acquired urinary tract infection, and combinations thereof. In one embodiment, the infection according to the present disclosure is caused by bacteria that exhibit antibiotic resistance. In certain embodiments, the infection is caused by a bacterium as listed above in the targeted bacterium. In another embodiment, an infection according to the present disclosure is caused by a bacterium expressing a toxin, such as a Shiga-toxin. In certain embodiments, the infection is Shiga-toxin producing E. coli (STEC) caused by

The disease or disorder caused by the bacteria may also be a metabolic disorder, such as obesity and/or diabetes. Accordingly, the present disclosure also relates to a pharmaceutical or veterinary composition disclosed herein for use in the treatment of metabolic disorders, including, for example, obesity and/or diabetes. A method for treating a metabolic disorder comprising also administering a therapeutically effective amount of a pharmaceutical or veterinary composition as disclosed herein, and a pharmaceutical or medicament disclosed herein for the manufacture of a medicament for treating a metabolic disorder; to the use of the veterinary composition.

A disease or disorder caused by bacteria may be a pathology in which bacteria of the human microbiome are involved. Accordingly, in certain embodiments, the present disclosure relates to a pharmaceutical or veterinary composition disclosed herein for use in the treatment of conditions in which bacteria of the human microbiome are involved, such as inflammatory and auto-immune diseases, cancer, infections or brain disorders. will be. A method for treating a condition involving bacteria of the human microbiome comprising administering also a therapeutically effective amount of a pharmaceutical or veterinary composition disclosed herein, and a drug for treating a condition involving bacteria of the human microbiome to the use of a pharmaceutical or veterinary composition disclosed herein for the manufacture of Indeed, without triggering any infection, some bacteria in the microbiome may secrete molecules that lead to and/or enhance the development of inflammatory or autoimmune diseases or cancers. More particularly, the present disclosure also discloses the efficacy of immunotherapy based, for example, on CAR-T (chimeric antigen receptor T) cells, TILs (tumor infiltrating lymphocytes) and Tregs (regulatory T cells) also known as T cell suppressors. It relates to controlling the composition of the microbiome to improve it. Modulation of microbiome composition to improve the efficacy of immunotherapy may also include immune checkpoint inhibitors well known in the art such as, but not limited to, PD-1 (programmed cell death protein 1) inhibitors, PD-L1 (programmed death ligand 1). ) inhibitors and CTLA-4 (cytotoxic T lymphocyte associated protein 4).

Some bacteria in the microbiome may also secrete molecules that will affect the brain, such as serotonin and melatonin for use in the treatment of depression, dementia or sleep disorders.

Therefore, a further object of the present disclosure is a method for controlling the microbiome of a subject comprising administering to said subject an effective amount of a pharmaceutical or veterinary composition as disclosed herein.

In certain embodiments, the present disclosure also relates to a method of personalized treatment for a subject in need thereof for a disease or disorder such as a bacterial infection, i) obtaining a biological sample from the subject and determining a group of bacterial DNA sequences from the sample to do; ii) identifying, based on the determination of the sequence, one or more pathogenic bacterial strains or species present in the sample; and iii) administering to the subject a pharmaceutical or veterinary composition according to the present disclosure capable of recognizing each pathogenic bacterial strain or species identified in the sample and capable of delivering a packaged plasmid. The present disclosure also relates to a pharmaceutical or veterinary composition according to the present disclosure for use in the treatment of a disease or disorder such as a bacterial infection, wherein the pharmaceutical or veterinary composition comprises i) obtaining a biological sample from the subject to be treated and obtaining from the sample a bacterial DNA sequence. determining a group; ii) identifying, based on the determination of the sequence, one or more pathogenic bacterial strains or species present in the sample, and iii) a pharmaceutical capable of recognizing each pathogenic bacterial strain identified in the sample and capable of delivering a packaged plasmid or through a method comprising the step of preparing a veterinary composition.

In one embodiment, the biological sample comprises pathological and non-pathological bacterial species and after administering to the subject a pharmaceutical or veterinary composition according to the present disclosure, the amount of pathogenic bacteria on or in the subject is reduced, The amount of non-pathogenic bacteria is not reduced.

In another specific embodiment, the present disclosure relates to a pharmaceutical or veterinary composition according to the present disclosure for use to improve the effectiveness of a drug. Indeed, without being pathogenic by themselves, it is known that some bacteria in the microbiome can metabolize drugs and transform them into ineffective or harmful molecules.

In another specific embodiment, the present disclosure provides at least one additional active ingredient, for example, a prebiotic and/or a probiotic and/or an antibiotic, and/or another antibacterial or antibiotic, and/or a bacterium. To a composition which may further comprise an optional agent for enhancing the targeting of the bacterial delivery vehicle and/or the delivery of the payload to the bacteria.

As used herein, "prebiotic" refers to a component that allows for a specific change in both activity and/or composition in the gastrointestinal microflora that can confer a benefit to the host. The prebiotic may be an edible food or a component thereof. A prebiotic may be a component that is optionally fermented. Prebiotics may contain complex carbohydrates, amino acids, peptides, minerals, or other essential nutrients for the survival of the bacterial composition. Prebiotics include, without limitation, amino acids, biotin, fructo-oligosaccharides, galacto-oligosaccharides, hemicellulose (eg, arabinoxylan, xylan, xyloglucan, and glucomannan), inulin, chitin, lactulose, mannan oligosaccharides , oligofructose-concentrated inulin, gums (eg guar gum, gum arabic and carrageenan), oligofructose, oligodextrose, tagatose, resistant maltodextrin (eg resistant starch), trans -galactooligosaccharides, pectins (e.g. xylogalactouronan, citrus pectin, apple pectin, and rhamnogalacturonan-I), dietary fiber (e.g. soybean fiber, sugar beet fiber, pea fiber, corn bran, and oat fiber) and xylooligosaccharides.

As used herein, "probiotic" refers to a dietary supplement based on living microorganisms that, when ingested in appropriate amounts, has a beneficial effect on the host organism by enhancing the intestinal ecosystem. A probiotic may comprise a non-pathogenic bacterial or fungal population, eg, an immunomodulatory bacterial population, such as an anti-inflammatory bacterial population, in the presence or absence of one or more prebiotics. They contain a sufficiently high number of viable and active probiotic microorganisms capable of exerting a balancing action on the gut flora through direct colonization. For the purpose of this description, the term "probiotic" refers to any biologically active form of a probiotic, preferably without limitation lactobacilli , bifidobacteria , streptococci , entero. It is understood to include enterococci , propionibacteria or saccharomycetes and also other microorganisms constituting the normal intestinal flora, or also fragments of the bacterial wall or DNA of these microorganisms. These compositions are advantageously suitable for safe administration to human and other mammalian subjects and are effective in the treatment, prevention of diseases or disorders caused by bacteria such as bacterial infections. Probiotics include, but are not limited to, Lactobacilli, Bifidobacteria, Streptococci, Enterococci, Propionibacteria, Saccharomycetes, Lactobacilli, Bifidobacteria, or Proteobacteria.

Antibiotics include penicillins such as penicillin G, penicillin K, penicillin N, penicillin O, penicillin V, methicillin, benzylpenicillin, nafcillin, oxacillin, cloxacillin, dicloxacillin, ampicillin, amoxicillin, pivampicillin, hetacillin , bcampicillin, methampicillin, talampicillin, epicillin, carbenicillin, ticarcillin, temocillin, mezlocillin, and piperacillin; cephalosporins such as cefacetril, cefadroxil, cephalexin, cephaloglycin, cephalonium, cephaloridin, cephalotin, cefapyrin, cefatrizine, cefazaflu, cepazedone, cepazolin, cepra Dean, ceproxadine, ceftezol, cefaclor, cefoniside, cefprozil, cefuroxime, cefuzonam, cefmetazole, cefothetan, cefoxitin, loracarbef, cefbuperazone, cefminox, sephotetan, cefoxitin, cefotiam, cefkafen, cefdaloxime, cefdinier, cefditoren, cepetamet, cefixime, cefmenoxime, cefodizim, cefotaxime, cefobecin, cefimizole, cef Podoxime, cefteram, ceftamere, ceftibuten, ceftiofur, ceftiolene, ceftizoxime, ceftriaxone, ceferazone, ceftazidim, latamoxef, cefclidine, cefepime, ceftazidim Fluprenam, Sepfocelis, Sepfozofran, Cefpyrome, Cefquinom, Flomoxef, Ceftobiprol, Ceftarolline, Ceftolozane, Cephaloram, Cefafarol, Cefkanel, Cefkanelpedrolor , cempidone, cepetrizole, cefibitril, cefmatylene, cefmepidium, cepoxazole, ceprotyl, cefsumide, ceftioxide, cefuracetim, and nitrocepine; polymyxins such as polysporin, neosporin, polymyxin B, and polymyxin E, rifampicins such as rifampicin, rifapentin, and rifaximin; Fidaxomycin; quinolones such as synoxacin, nalidixic acid, oxolinic acid, pyromidic acid, pipemidic acid, rosoxacin, ciprofloxacin, enoxacin, pleloxacin, romefloxacin, nadifloxacin, norfloxacin, ofloxacin, pefloxacin Reaper, Lufloxacin, Valofloxacin, Grepafloxacin, Levofloxacin, Pajufloxacin, Temfloxacin, Tosufloxacin, Clinafloxacin, Gatifloxacin, Gemifloxacin, Moxifloxacin, Cytafloxacin, trovafloxacin, prulifloxacin, delafloxacin, nemonoxacin, and zavofloxacin; sulfonamides such as sulfafurazole, sulfacetamide, sulfadiazine, sulfadimidine, sulfafurazole, sulfisomidine, sulfadoxine, sulfamethoxazole, sulfamoxol, sulfanitran, sulfadimethoxine, sulfamethoxypyri minced, sulfamethoxydiazine, sulfadoxine, sulfamethopyrazine, and terephthyl; macrolides such as azithromycin, clarithromycin, erythromycin, fidaxomycin, telithromycin, carbomycin A, zosamycin, kitasamycin, midecamycin, oleandomycin, solithromycin, spiramicin, troleandomycin, tylosine, and loxithromycin; ketolids such as telithromycin, and setromycin; fluoroketolids such as solithromycin; lincosamides such as lincomycin, clindamycin, and pilimycin; tetramycins such as demeclocycline, doxycycline, minocycline, oxytetracycline, and tetracycline; aminoglycosides such as amikacin, dibecacin, gentamicin, kanamycin, neomycin, netylmicin, sisomicin, tobramycin, paromomycin, and streptomycin; ansamycins such as geldanamycin, herbimycin, and rifaximin; carvacepem such as lauracarbev; carbapenems such as eltapenem, doripenem, imipenem (or cilastatin), and meropenem; glycopeptides such as teicoplanin, vancomycin, telavancin, dalbavancin, and oritabancin; lincosamides such as clindamycin and lincomycin; lipopeptides such as daptomycin; monobactams such as aztreonam; nitrofurans such as furazolidone, and nitrofurantoin; oxazolidinones such as linezolid, fosizolid, radezolid, and torezolide; teixobactin, clofazimine, dapsone, capreomycin, cycloserine, ethambutol, ethionamide, isoniazid, pyrazinamide, rifabutin, alsfenamine, chloramphenicol, fosfomycin, fusidic acid, metronidazole, mupyrosin , platensimicin, quinupristine (or dalfopristine), thiamphenicol, tigecycline, tinidazole, trimethoprim, alatrofloxacin, fidaxomycin, nalidixic acid, rifampin, derivatives and combinations thereof. can be selected from

In certain other embodiments, the present disclosure relates to the in situ bacterial production of any compound of interest, including therapeutic compounds such as prophylactic and therapeutic vaccines for mammals. The compound of interest may be produced inside the targeted bacterium, secreted from the targeted bacterium, or expressed on the surface of the targeted bacterium. In a more specific embodiment, the antigen is expressed on the surface of a targeted bacterium for prophylactic and/or therapeutic vaccination.

The present disclosure also relates to non-therapeutic uses of bacterial delivery particles. For example, a non-therapeutic use may be a cosmetic use or a use for improving the well-being of a subject, particularly a subject not afflicted with a disease. Accordingly, the present disclosure also relates to cosmetic or non-therapeutic compositions comprising the bacterial delivery particles of the present disclosure.

The present invention can be further illustrated through the following examples.

Example

Example 1

The lambda-packaged cosmid is derived from lambda PaPa, a variant of the wild-type Ur-lambda phage [7] with a frameshift mutation in the stf gene resulting in a truncated protein that is an inactivating STF protein [7], i.e. a protein with no biological activity. . Lambda Papa has been used as a de facto wild-type lambda phage in most laboratory studies, as it produces larger plaques that are easier to handle, as opposed to wild-type Ur-lambda. The stf gene encodes a flanking tail fiber protein that, in the case of phage lambda, recognizes a secondary receptor on the cell surface, OmpC.

These secondary receptors allow transient binding of phage particles on the cell surface to scan on the surface and locate the injection mechanism in contact with the primary receptor (LamB in the case of lambda, an interaction mediated by the lambda protein gpJ). Since STF binding is reversible, it allows the phage to "walk" on the cell surface until the primary receptor is found and the infection process begins. These protein complexes are sometimes referred to as “L-shaped fibers” such as in T5, such as “lateral tail fibers” in lambda, “long tail fibers” in T4, or tail spikes, such as in phage P22 [7]-[10]. In some phage cases, the presence of this second set of proteins is essential for the infectious process to occur, eg in the T4 case [8]. In some other phages, such as lambda, this second set of proteins is not required for the infectious process to occur, but may allow for more efficient attachment to target cells [7].

The wild-type length of the lambda phage genome is 48.5 kbp. It is well known that lambda can only package 37.7 kbp to 51 kbp DNA [11], or about 78% to 105% of its wild-type length. Smaller DNA payloads do not create enough pressure inside the capsid for packaging termination to occur and larger ones make the capsid too unstable. Additionally, smaller genomes were found to be excreted with much lower efficiency in the presence of higher external osmotic pressure [12], and as the length of the encapsidated DNA was reduced, the efflux force was found to be two size extremes 37.7 to 51 kbp. decreases in an exponential manner. It is also known that the smaller the payload, the lower the efficiency with which packaged particles are formed [11]. Combining these two observations, it can be concluded that smaller DNA payloads are detrimental if sufficiently high titers are required for in vivo experiments.

Most phagemids packaged using the lambda system (and many others) are much smaller than the wild-type length of the phage. Packaging is possible because the cosmid forms a concatimer via the sigma replication pathway, and if the concatimer is between 74% and 105% of the length of the wild-type lambda genome, packaging is terminated and a mature packaged cosmid is formed [ 11]. This means that, in contrast to the wild-type lambda genome, the idle particles will not have a homogeneous length of packaged DNA, resulting in a 74% to 105% genome length of the full range. For example, in [11] a 12.8 kb cosmid was found to be packaged as trimers and tetramers, corresponding to 38.4 kb and 51.2 kb (accepted packaging size range), and the 38.4 kb variant was found to be about 15 of the particle. %, but 51.2 kb was found in about 40%. Similarly, 4.6 kb plasmids can be packaged as 9-mer, 10-mer and 11-mer (41.4 kb, 46 kb and 50.6 kb). In this case, the 41 kb variant was most common with about 20% of particles having this size followed by about 12% being the 50.6 kb variant, and the 46 kb variant being present in only about 5% of the particles.

For in vivo applications, such as oral delivery of encapsidated DNA particles, the packaged phagemid may need to be provided in a concentration high enough to reach all target cells, so a payload that provides a sufficiently high titer in vivo It is essential not only for my actives, but also for optimizing the production process.

Finally, in addition to a payload of suitable size, the packaged particle needs to be able to bind its target cell sufficiently long and strongly for the injection process to take place. It was previously confirmed that the presence of STF is not essential for lambda-mediated in vitro transduction experiments in K-12 laboratory strains [7].

For that reason, we expect that functional lateral tail fibers, as well as suitable payloads packaged as concatemers of the correct length, significantly increase the efficiency when performing in vivo delivery assays of lambda-based packaged phagemids. was confirmed not to. We also found that for strains other than the model K12 strain, STFs and/or gpJs from other phages capable of specifically recognizing the surface antigen/receptor of the target bacteria mediate efficient delivery both in vitro and in vivo. demonstrated unexpectedly that it can be used to generate engineered lambda virus particles.

E. with engineered streptomycin resistance. A mouse model using E. coli strain MG1655, or derivatives, was developed using established colonization protocols [13]. Mice (Balb/c ByJ, 　 7 weeks) were treated with a short course of streptomycin, which is known to reduce the native E. coli gut population [14]. This treatment preserves the other species present in the natural microflora, while maintaining the presence of exogenous E. Allow coli to be administered and colonize the empty ecological niche. Specifically, animals were treated with a 5-day course of 5 g/L streptomycin in drinking water. Treatment was stopped 5 days prior to gavage of packaged phagemids to avoid any bias and selection of resistance due to antibiotic evolution pressure.

To test the in vivo delivery efficiency of packaged phagemids, psgRNAcos, a 2.5 kb cosmid of SEQ ID NO: 1 carrying a kanamycin resistance gene, was packaged into lambda PaPa particles, encoding a non-functional stf gene. To achieve this, the cosmid possesses the lambda prophage lacking the cos site, but retains all mechanisms for induction of the lambda phage lysis cycle, including the DNA packaging system. E. coli strains were transformed. The cI repressor of lambda prophage carries a mutation that makes it thermosensitive and allows induction of the lysis cycle through temperature changes. Cells containing lambda cosmid were grown at 30°C in liquid LB medium. At an OD600 of 0.6, the culture was transferred to 42° C. for 25 min to induce entry into the lysis cycle. Thereafter, the cells were again transferred to 37° C. for 3 h to allow assembly of virions containing lambda cosmid. Cells were then centrifuged and washed in lambda buffer (10 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgSO ₄ ). Chloroform was added and the sample was spun down at 17,000 g for 5 min. Finally, the aqueous layer was collected and filtered through a 0.2 μm pore-size filter. The titer of the packaged phagemid was determined by performing an in vitro transduction assay using strain MG-GFP as recipient. MG-GFP is a derivative of strain MG1655 having an ampicillin resistance gene and a gfp fluorescent reporter gene inserted into the chromosome. The titer was determined to be about 10 ⁶ particles/μL (not shown). This packaged phagemid stock was used to transduce strain MG-GFP in the gut of mice colonized with MG-GFP for 5 days. Feces were collected at different time points, homogenized and plated on LB agar plates in the presence of kanamycin to monitor the number of transduced MG-GFP cells. Kanamycin-resistant colonies were counted in different parts of the mouse intestinal tract (psgRNAcos encodes the kanamycin marker). As shown in FIG. 1 , few detectable transfectants were observed anywhere.

The observed low transduction efficiency was very high in the digestive tract of mice colonized by lambda PaPa, MG-GFP colonized by MG-GFP and measured by counting plaque forming units over time in the feces of mice infected with lambda PaPa. consistent with the fact that they are insufficiently replicated. To determine the reason for this insufficient efficiency of lambda PaPa in the mouse gut, an in vivo evolution assay was performed to select improved variants of these phages. Mice colonized with MG-GFP were gavaged with a total starting dose of 10 ⁷ lambda PaPa. Every day, feces were collected and tested for their ability to form plaques on lawns of MG1655. They were also homogenized to 40 mg/mL in IX PBS and filtered through 0.22 μm pore membranes to refeed the mice. As shown in Figure 2, lambda PaPa was unable to bind to its host in the mouse gut, so there was no PFU actually detected initially. Some peaks were observed with the amount of PFU-forming particles oscillating from high to undetectable levels by day 17, where a steady increase in the number of PFUs was detected.

Remarkably, when the genome of the phage blotter obtained on day 35 was sequenced, it was observed that the ORF of the stf gene was restored (although it has mutations among K338E, T391M and A395S for the canonical STF sequence of SEQ ID NO: 14) The lambda PaPa particle had an inverted mutation to give the full-length stf gene. Additionally, the gpJ gene, responsible for binding to the LamB receptor, was also observed to be mutated at three positions. These mutations were tested on wild-type MG1655 and MG1655-delta-LamB to determine whether they altered the receptors used by these phages. We understood that there was no difference, which confirmed that the new Ur-lambda particles still use LamB as their primary receptor.

A DNA payload with a size of 3 kb, using the ORF of the stf gene restored from the original lambda packaged phagemid producing strain, which retains the original STF sequence of SEQ ID NO: 14 but not the mutated form obtained in the above experiment. The same experiment was performed with a packaged phagemid containing (pJ23104-GFP of SEQ ID NO: 2) but this time encoding the chloramphenicol marker. The sequence of the gpJ gene was not changed at this stage. The packaged phagemids were gavaged to mice colonized with MG1655-Strp (with streptomycin resistance). Feces were homogenized to 40 mg/mL in IX PBS and plated on Drigalski plates supplemented with or without 25 μg/mL chloramphenicol. The amount of chloramphenicol-resistant cells was counted in the feces for up to 48 hours after transduction. As can be seen in FIG. 3 , the amount of transduced cells was increased compared to the lambda PaPa packaged phagemid, but the transfer efficiency was very low (about 1 in 1000 cells). When the same experiment was attempted with packaged Ur-lambda particles having the gpJ gene modified to mimic the particles obtained in the in vivo evolution assay, the same results were observed. As no significant differences were identified in in vitro transduction assays, it was concluded that other factors must be present preventing the packaged phagemids from functioning optimally in vivo.

An experiment was conducted in which a packaged phagemid having a larger DNA payload in the Ur-lambda capsid, pJF1 of SEQ ID NO: 3 (approximately 7 kb) was produced and administered to mice. It is important to note that the gpJ gene is not altered, so these particles encode the wild-type gpJ lambda of SEQ ID NO: 10 and still use LamB as their primary receptor. The same protocol was followed for in vivo delivery to the smaller DNA payload of SEQ ID NO: 2. Surprisingly, as can be seen in FIG. 4 , the addition of both the functional stf gene and the packaged DNA payload to a higher titer thanks to its length dramatically increases the delivery efficiency in vivo.

As strong differences were observed depending on the size of the DNA payload used, a set of experiments in which DNA payloads of different sizes of SEQ ID NOs: 4 to 8 were packaged in a lambda capsid delivery vehicle and their titers were assessed prior to in vivo assays. was performed. Production was performed under the same conditions for all payloads. After production and lysis according to the aforementioned protocol, an STF filtration step with 100 kDa pore size was performed on the clear lysate and buffer exchanged against IX PBS. The TFF step is allowed for a particle concentration of about 1 log compared to the clear lysate. As can be seen in Figure 5, smaller DNA payloads (2.2 kb) are produced at titers as low as at least 1 log compared to their larger counterparts in our production system.

Finally, to validate the role of DNA payload size, counterweight and STF presence, the 6 kb and 8 kb variants shown in Figure 5 (GG6K of SEQ ID NO: 6 and GG8K of SEQ ID NO: 7, respectively) were two Lambda phagemid form: lambda particles containing its chaperone protein and intact total STF and lambda particles produced in a strain in which the stf and tfa chaperone genes were uniformly removed from the prophage genome were packaged. Both forms encode the wild-type lambda gpJ gene of SEQ ID NO: 10. After clarification, the lysates were passed through a TFF instrument equipped with a 100 kDa filter as described for the phagemids in FIG. 5 and their titers were determined (ca. 10 ⁸ /μl). A total of 10 ¹⁰ packaged phagemids of each DNA payload were gavaged into mice and delivery efficiencies were calculated as described above. Remarkably, as can be seen in Figure 6A, the addition of functional STF significantly increases the in vivo delivery efficiency, in some cases 100%.

Significantly, these results do not reflect the results obtained in vitro when no apparent differences were observed using lambda Papa or Ur-lambda packaged phagemids through plating, and the results of functional STFs in in vivo conditions. emphasize the importance

This set of experiments brings three conclusions that have major implications for the development of successful approaches for using packaged phagemids in vivo, especially for decolonization purposes. First, a DNA payload of suitable size is needed to obtain a sufficiently high titer. If the DNA payload is too small, it may be necessary to run an intensive downstream process that concentrates the concentration of particles to a suitable titer, which may slow down the production process and increase its cost. Second, it has been previously confirmed that the presence of functional STFs is essential to obtain the high delivery efficiency required for in vivo experiments, and that STFs can be engineered for each target strain (e.g., U.S. Provisional Application No. 62/802777). , U.S. Application No. 16/696,769, and U.S. Application No. 16/726,033, each of which is incorporated herein by reference in its entirety). Third, in this case the primary receptor, LamB, may not be optimal for the application sought. It has been shown that phagemid particles can be engineered to include gpJ variants (in the case of lambda) that bind to receptors other than LamB, as described below.

Manipulation of the lambda gpJ protein

Lambda phages use the bacterial LamB OMP as their main entry receptor, recognized by the gpJ protein located at the tip of the phage particle [15]. These events trigger DNA export into the bacterial cytoplasm and are generally considered "irreversible" binding processes [15]. However, bacterial strains can become resistant to lambda phage entry if the LamB receptor is mutated, masked, or downregulated; In particular, downregulation of the LamB gene was observed for the MG1655 strain in some mouse models, and this downregulation is caused by genotype changes, and mutations in the malT gene, which regulate LamB expression levels, lead to a sharp decrease in the number of these receptors in the membrane. causes [16], [17]. Eventually, bacteriophages have evolved different strategies to bypass these defense mechanisms. For example, mutagenesis of the gpJ protein allows them to use different receptors [18], [19]. It is also known that the receptor-recognition activity of gpJ resides in its C-terminal portion, and that fragments as small as 249 aa confers the ability to bind to the LamB receptor [5].

Therefore, having different combinations of STF and gpJ that allows entry into different bacterial strains or particular conditions is crucial for the successful use of lambda-derived packaged phagemids as therapeutics, especially for in vivo applications. For this reason, some gpJ chimeras, while others, are not. Engineered to recognize receptors other than LamB, including identified variants that allow entry in E. coli strains.

To achieve this, different teeth. The gpJ homologue from the E. coli prophage genome was searched and protein alignment was performed to find a region within the C-terminal portion that could be involved in recognizing the bacterial OMP receptor (Fig. 7).

As shown in Figure 7, all gpJ variants share high sequence identity in most of their lengths (about 820 amino acids or less) except for their C-terminus. To verify that receptor specificity is contained within this region of the gpJ protein, different fusions were tested using two insertion points (SEQ ID NO: 42 and SEQ ID NO: 43) as shown in FIG. 7 . These chimeras are different. Derived from E. coli prophages, their C-terminus is different and the N-terminus of lambda gpJ is shared. The gpJ variant was uniformly inserted into the packaged phagemid and lambda producing strain containing the DNA payload p7.3kb of SEQ ID NO: 9 (coding for GFP and chloramphenicol resistance genes) produced as described above. Four strains were used to titrate these packaged phagemids: MG-GFP (a variant of MG1655 encoding GFP in the genome), MG-delta-LamB (KEIO variant lacking the LamB receptor), H10-waaJ, O157 O157 strain lacking waaJ, preventing expression of the capsular antigen [20], and MG1656-OmpCO157, a modified MG1655 exchanged for the OmpC variant present in the O157 strain. The apparent titer identified in Figure 8 is used as a measure of the efficiency of the packaged phagemids, since the OD600 of all strains remained constant (0.8). As can be seen in Figure 8, both insertion points yielded functional gpJ chimeras, but surprisingly the recognized receptor was changed from that originally recognized by lambda gpJ; For example, as shown in Figure 8A, variant H591 of SEQ ID NO: 11 now employs OmpC. Interestingly, the two gpJ variants (Z2145 of amino acid sequence SEQ ID NO: 12 and 1A2 of amino acid sequence SEQ ID NO: 13) showed reduced or virtually no entry in MG1655, respectively, while they were present in the O157 strain. can recognize receptors that 8). As the MG-GFP and MG-delta-LamB strains have identical titers, none of the variants use the LamB receptor (Fig. 8B). Finally, another gpJ variant was constructed and named A8 (SEQ ID NO: 49) and its delivery efficiency was tested in MG1655 (containing its endogenous OmpC variant) and MG1656-OmpCO157. To do this, serial 1:3 dilutions of phagemids containing the A8 or 1A2 gpJ variant and the P2-stf chimera (typically the protein of sequence SEQ ID NO: 50, encoded by the nucleic acid sequence SEQ ID NO: 56) Water was incubated with a fixed amount of MG1655 or MG1656-OmpCO157 cells with an OD600 = 0.025; This was done to obtain different MOIs. After incubation at 37° C. for 45 minutes, GFP levels at each MOI were measured using a flow cytometer and plotted against the MOI. As previously observed, the 1A2 gpJ variant can only recognize the receptor at MG1656-OmpCO157; Surprisingly, the A8 variant was able to recognize both OmpC receptors in MG1655 and MG1656-OmpCO157.

In view of these results, it appears that the approach to generating gpJ chimeras can produce not only variants that recognize different OMPs in the same strain, but also variants that show different strain specificities.

However, changing the primary receptor is a futile effort if the strain is encapsulated, as the packaging phagemid will not have access to the receptor due to the physical shielding of the cell surface, so the delivery efficiency will be drastically reduced. The reason is that in the case of the activity test of the gpJ fusion shown above, the naked H10-waaJ O157 strain was used, as the O157 strain is known to produce a group IV capsular antigen that masks the cell surface [21]. In this case, a combination of functional STF and gpJ is necessary to obtain high delivery efficiency.

To accomplish this, the stf and tfa (chaperone) genes from the lambda prophage in the production strain were uniformly deleted. The chimeric stfs were then complemented in trans by plasmids carrying the DAPG-inducible forms of these chimeric stfs. Indeed, when using the STF variant represented by SEQ ID NO: 16 and designated as WW11.2, which shows specificity for the O157 antigen, in combination with the gpJ variant Z2145 of the amino acid sequence SEQ ID NO: 12 shown above, the encapsulated O157 strain A very efficient entry was obtained, as can be seen in Fig. 8C.

Manipulation of lambda tape measure protein, gpH

Additionally, it was identified that another possible mechanism for protecting bacteria from phage injection is mutations in periplasmic proteins that are thought to aid in the formation of channels across the periplasm during DNA injection [22]-[24]. Although the exact location of these mutations is unknown, lambda phage can reacquire its activity in these mutants by modifying the tape measure protein, gpH gene. In lambda phage, the protein involved in the interaction with the mannose permease complex is gpH. Recently, a mutation in the glucose transporter protein PtsG has made it resistant to infection by a lambda-type phage, HK97; It has been described that the gpH protein of HK97 is inhibited and that injection cannot occur with these mutants. The authors describe that by changing the region of the gpH protein in the HK97 phage they can bypass the PtsG mutation and the engineered HK97 becomes infective again.

To test whether this hypothesis is generalizable to lambda phages, E. Alignment of the lambda gpH protein to other lambda-type prophages present in E. coli was performed. As shown in Figure 9A, other variants of gpH can be found where there is an extremely high percentage of identity over the length of gpH, with the exception of two regions showing heterogeneity in their sequences (denoted distal and proximal). .

The gpH gene was modified in the lambda packaged phagemid production strain, as was done for the gpJ variant, to include both variable regions, and the packaged phagemids were produced as described above. The gpH variant of SEQ ID NO: 24 was designated gpH-IAI. In this case, three strains were used for titration: MG1655, KEIO manY and KEIO manZ, containing deletions of two components of the mannose permease complex. As can be seen in FIG. 9B , deletion of manZ and manY resulted in a 2-log decrease in apparent titer compared to MG1655, but the use of the modified gpH lambda phage variant of SEQ ID NO: 24 also in the manZ and manY strains, its Fully restores activity. These results show that modification of the gpH gene in lambda phage can be used to allow entry or improve entry in strains showing defects or changes in the permease complex.

In a series of experiments, lambda-type phage recognition and injection into its host: capsule/secondary receptor (via STF); primary receptor recognition (via gpJ); and permease/periplasmic channel formation (via gpH). These strains have a wide range of lice. It can be used to engineer packaged phagemids and lambda-type phages with modified tropism and injection efficiency in E. coli strains.

Use of lambda phagemids for efficient delivery to different, unmodified proteobacteria

Finally, lambdatype-derived packaged phagemids were obtained from E. It has been shown that it can be used for delivery in other proteobacteria different from E. coli, such as Klebsiella, Agrobacterium, or Pseudomonas [25]-[27]. However, this approach is Transformation of the recipient strain with a plasmid encoding the E. coli LamB receptor is required as other species do not possess it. Although this is a validated approach for cells grown in laboratory conditions, it becomes impractical if bacteria present in natural conditions are targeted with lambda-derived packaged phagemids (e.g., the gut), where the plasmid encoding the receptor is easily Because it cannot be transmitted.

It was tested whether the lambda-derived packaged phagemid could be used to deliver the p7.3 kb payload of SEQ ID NO: 9 to other proteobacteria, such as Enterobacter. To achieve this, the different lambda packaged phagemids are gpJ, Z2145 of SEQ ID NO: 12 and 1A2 of SEQ ID NO: 13 and the STF variant STF-EB6 of SEQ ID NO: 20 (its of SEQ ID NO: 20) chaperone (or auxiliary) protein present), STF75 of SEQ ID NO: 17 (with its auxiliary protein of SEQ ID NO: 18) and STF23 of SEQ ID NO: 21 (with its auxiliary protein of SEQ ID NO: 22) several engineered to contain the combination. do 10 is this. While retaining the same principles as observed for E. coli strains, the efficiency of transfer is strongly dependent on the choice of gpJ and STF used, and in some combinations, entry in these bacteria is inefficient (transducers Although easily ascertainable), changes in STF and gpJ allow for much higher transduction efficiencies.

These results indicate that the injection components (gpJ, stf, gpH) of the lambda capsid were found in E. It shows that it can be engineered to achieve high transfer efficiency in other types of bacteria other than E. coli. Accordingly, the present inventors have proposed one or more of these approaches (optimizing DNA payload size and selection of appropriate gpJ/STF/gpH combinations) to generate optimized lambdatype-based delivery vehicles for use in in vivo experiments involving delivery of DNA. It has been shown to be advantageous to use

Example 2

Use of lambda phagemids for efficient delivery in bacteria in vivo

The present inventors present that the packaged phagemid internal payload with a lambda STF fusion based on the point of homology is delivered to bacteria in the gastrointestinal tract of the host without such STF affected by the proteolytic activity present in the gastrointestinal tract, in particular by pancreatin. It was tested whether it could be (i.e., low or no delivery of the payload to the bacteria).

In general, the structure of the homology-based STF chimera should be reminiscent of the original protein, since the amino acid sequence of the fusion point is not extremely modified. This means that if the original STF evolved to pancreatin resistance, the chimera is also likely to be resistant. To prove this, the present inventors have LMR_503 strain (lambda-STF29, SEQ ID NO: 63, with its helper protein of SEQ ID NO: 65; and lambda-, with its helper protein of SEQ ID NO: 66; Two different STF chimeras were engineered with lambda STF functionality when targeting STF118, SEQ ID NO: 64). The insertion point of STF29 is ADAKKS (SEQ ID NO: 38) and the insertion point of STF118 is MDETNR (SEQ ID NO: 39). Eligobiotics® and respective STF chimeras carrying 1A2 gpJ were produced and titrated against MG1656-OmpCO157 or LMR_503 after treatment with or without pancreatin at pH 6.8. Briefly, the read strain for chimeric STF activity is LMR_503 and the read for gpJ activity is MG1656-OmpCO157. As can be seen in Figure 11, these STF chimeras based on the homology points do not actually show degradation in the presence of pancreatin, as predicted by the inventors based on the STF chimera homology-designed fusion points.

Example 3

Effect of DNA Payload Size on Eligobiotics® Packaging

To evaluate the effect of DNA payload size on the number of payloads packaged in Eligobiotics® (EB), three different payloads were used to produce Eligobiotics® as summarized in Table 1.

Table 1 : Batch of Eligobiotics® Produced

After fermenting, dissolving (0.1% Triton X-100, 3 hours incubation at 37°C in the presence of 2000 U/L Benzonase) and clearing in Zeta Plus capsules (3M), Eligobiotics® is anionic in Sartobind Q capsules (Sartorius). Purification via exchange chromatography. This initial purification was followed by buffer exchange and concentration steps via tangential flow filtration on a Pellicon 2 minicassette Biomax 300 kDa (Millipore). A final polishing step of size exclusion chromatography was performed on Sepharose 6FF resin (GE Healthcare) to yield purified Eligobiotics.

Analysis of Eligobiotics® DNA content was performed via analytical ultrafiltration on a Beckman Coulter Optima AUC using an AN50Ti rotor at 6 krpm. The sedimentation coefficients of the different EBs present in solution for each EB batch were extracted from the sedimentation rate data (obtained at 260 and 280 nm).

Based on their sedimentation coefficients and molecular weights calculated from their 260/280 nm ratio, different populations of detected EBs produced either 3 copies (focused at 290 S) or 4 copies (focused at 330-340 S) of the payload. could be isolated as Eligobiotics® containing 12).

Significant differences were observed between Eligobiotics® depending on the size of the packaged payload. Although Eligobiotics® packaging smaller p1392 (11.615 kb) almost exclusively yielded particles containing a payload of 4 copies, a small increase in the size of the payload (below 800 bp) is a shift towards 3-copy packaging. have to do with As such, Eligobiotics® produced with p779 (12.428 kb) preferentially packaged a payload of 3 copies, whereas approximately 1/3 of the EBs contained a payload of 4 copies (Fig. 13).

Thus, p1392 appears close to the ideal size for exclusively packaging a 4 copy number of payloads in phage-derived capsids, yielding a homogeneous population. An increase in the size of the payload compared to p1392 resulted in a more heterogeneous Eligobiotics® population, with an increased proportion of EBs containing 3 copies of the payload. From this dataset, it appears that there is a lower limit for concatemer packaging close to 36 kb, as described in [28]. A p1085 of size 12.125 kb can package 3 copies per head (36.375 kb) or 4 copies per head (48.5 kb), but a 4 copy number paper is preferred as identified in FIG. 13 . The size increase to 12.428 kb allows packaging of 3 copies per head (37.284 kb) and 4 copies per head (49.712 kb), in which case 4 copies are preferred. From these two data points, we deduced that the lower limit for packaging is actually around 36 kb but the efficiency is low. A size increase right up to 909 bp completely shifts the species being packaged into 4 copies, and the limit for optimal efficiency of packaging, possibly driven by a pressure signal in the capsid, lies within these two sizes. Finally, the 11.615 kb payload actually packages only 4 copies per head (46.46 kb), as the 3-copy species is slightly below the packaging limit (34.845 kb), even at low efficiency.

From these data, we can also predict that the size provides for packaging of single and multimeric species, as shown in Tables 2 and 3 below. Smaller sizes that yield a single packaging species are generally preferred for several reasons, including ease of manipulation and low probability of introducing unwanted restriction sites. Finally, a size that allows a very efficient packaged species that is not too small (26-39 kb) or too large (50-51 kb) is also desirable in some cases, where the amount of DNA present in the capsid is reduced by pressure within the capsid. This is because it has been confirmed that it can change the packaging and stability of particles [29]-[30]. Finally, a size large enough to allow the production of packaged phagemids at high titers is also more particularly preferred.

Table 2 : Estimated number of concatemers packaged in capsids according to monomer size.

Shaded cells indicate better species and white cells indicate species that are either too small or too large for optimal packaging. The lower and upper limits for efficient packaging were set at 36 kb and 51 kb, respectively.

Table 3 : Estimated number of concatimers packaged in capsids depending on the monomer size from 9 to 13 kb.

Shaded cells indicate better species and white cells indicate species that are either too small or too large for optimal packaging. The lower and upper limits for efficient packaging were set at 36 kb and 51 kb, respectively.

References

<110> ELIGO BIOSCIENCE <120> BACTERIAL DELIVERY VEHICLES FOR IN VIVO DELIVERY OF A DNA PAYLOAD <130> B3419PC <160> 66 <170> PatentIn version 3.5 <210> 1 <211> 2528 <212> DNA <213> Artificial Sequence <220> <223> psgRNAcos (p184) <400> 1 tgcagcgcga tcgtaatcag gatcccatgg tacgcgtgct agaggcatca aataaaacga 60 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 120 ctgagtagga caaatccgcc gccctagacc ctccacgcac gttgtgatat gtagatgata 180 atcattatca ctttacgggt cctttccggt gatccgacag gttacggggc ggcgacctcg 240 cgggttttcg ctatttatga aaattttccg gtttaaggcg tttccgttct tcttcgtcat 300 aacttaatgt ttttatttaa aataccctct gaaaagaaag gaaacgacag gtgctgaaag 360 cgaggctttt tggggcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 420 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 480 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 540 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 600 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 660 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 720 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 780 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 840 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 900 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 960 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 1020 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 1080 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 1140 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgactagtg cttggattct 1200 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg 1260 aggtcattac tggatctatc aacaggagtc caagcgagct ctcgaacccc agagtcccgc 1320 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat 1380 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg 1440 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa 1500 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac 1560 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc 1620 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt 1680 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag 1740 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg 1800 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc 1860 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg 1920 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac 1980 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg 2040 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc 2100 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct 2160 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac 2220 cttaccagag ggcgccccag ctggcaattc cgacgtctaa gaaaccatta ttatcatgac 2280 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcgggccc attaagttct 2340 gtgctaggag gtgactgaag tatattttag gaattctaaa gatctttgac agctagctca 2400 gtcctaggta taatactagt tgagaccagt ctaggtctcg gttttagagc tagaaatagc 2460 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt 2520 tttggtag 2528 <210> 2 <211> 3274 <212> DNA <213> Artificial Sequence <220> <223> pJ23104-GFP (p211) <400> 2 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt 60 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc 120 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta 180 tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg 240 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact 300 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg gagtgtatac 360 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa 420 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct 480 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg 540 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca 600 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat 660 cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc 720 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg 780 cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga 840 ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct 900 tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt 960 tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg 1020 gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct 1080 tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac 1140 caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag atttcagtgc 1200 aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtactagt 1260 ggatttgaca gctagctcag tcctaggtat tgtgctagcg aattcattaa agaggagaaa 1320 ggtacccatg agtaaaggtg aagaactgtt caccggtgtt gttccgatcc tggttgaact 1380 ggatggtgat gttaacggcc acaaattctc tgttcgtggt gaaggtgaag gtgatgcaac 1440 caacggtaaa ctgaccctga aattcatctg cactaccggt aaactgccgg ttccatggcc 1500 gactctggtg actaccctga cctatggtgt tcagtgtttt tctcgttacc cggatcacat 1560 gaagcagcat gatttcttca aatctgcaat gccggaaggt tatgtacagg agcgcaccat 1620 ttctttcaaa gacgatggca cctacaaaac ccgtgcagag gttaaatttg aaggtgatac 1680 tctggtgaac cgtattgaac tgaaaggcat tgatttcaaa gaggacggca acatcctggg 1740 ccacaaactg gaatataact tcaactccca taacgtttac atcaccgcag acaaacagaa 1800 gaacggtatc aaagctaact tcaaaattcg ccataacgtt gaagacggta gcgtacagct 1860 ggcggaccac taccagcaga acactccgat cggtgatggt ccggttctgc tgccggataa 1920 ccactacctg tccacccagt ctaaactgtc caaagacccg aacgaaaagc gcgaccacat 1980 ggtgctgctg gagttcgtta ctgcagcagg tatcacgcac ggcatggatg aactctacaa 2040 ataaaacgca tgagaaagcc cccggaagat caccttccgg gggctttttt attgcgctcg 2100 ggtagcacca gagctcgccg cagccgaacg ccccaaaaag cctcgctttc agcacctgtc 2160 gtttcctttc ttttcagagg gtattttaaa taaaaacatt aagttatgac gaagaagaac 2220 ggaaacgcct taaaccggaa aattttcata aatagcgaaa acccgcgagg tcgccgcccc 2280 gtaacctgtc ggatcaccgg aaaggacccg taaagtgata atgattatca tctacatatc 2340 acaacgtgcg tggagggact agtggattcg ggtagcacca gaagtctata gcacgtgagc 2400 acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg 2460 ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc 2520 attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa 2580 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta 2640 agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc 2700 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag 2760 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag 2820 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac 2880 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag 2940 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc 3000 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg 3060 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt 3120 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga 3180 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg 3240 atttttttct ccattttagc ttccttagct cctg 3274 <210> 3 <211> 7240 <212> DNA <213> Artificial Sequence <220> <223> pJF1 (p344) <400> 3 gattttacgg ctagctcagt cctaggtaca atgctagcga atcattaaag aggagaaagg 60 tactatggca cgtaccccgt cacgtagtag cattggtagc ctgcgtagtc cgcataccca 120 taaagcaatt ctgaccagta ccatcgagat cctgaaagaa tgtggttata gcggactgag 180 cattgaaagc gttgcacgtc gtgccggagc aagcaaaccg accatttatc gttggtggac 240 gaataaagca gcactgattg ccgaagtgta tgaaaatgaa agcgaacagg tgcgtaaatt 300 tccggatctg ggtagcttta aagcagatct ggatttttta ctgcgtaatt tatggaaagt 360 ttggcgtgaa actatttgcg gtgaagcatt tcgttgtgtt attgcagaag ctcagctgga 420 tcctgcaacc ctgacccagt taaaggatca atttatggaa cgtcgtcgtg agatgccgaa 480 aaaactggtt gaaaatgcca ttagcaatgg tgaactgccg aaagatacca atcgtgaact 540 tcttctggat atgatttttg gtttttgttg gtatcgcctg ttaaccgaac agctgaccgt 600 tgaacaggat attgaagaat ttaccttcct tctgattaat ggtgtttgtc cgggtactca 660 gcgttaacta ggccataatc gctaccaaat tccagaaaac agacgctttc gagcgtcttt 720 tttcgttttg gtcacgacgt actgaatctg attcgttacc aattgacatg atacgaaacg 780 taccgtatcg ttaaggttac tggagcttaa aaaggagaaa gtttctatgg ataagaaata 840 cagcataggc ttagacatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata 900 taaagttccg tctaaaaagt tcaaggtact gggtaataca gatcgccata gtatcaaaaa 960 gaacttaatc ggtgcgcttc tgttcgattc cggcgaaacc gcagaagcaa cacgtctgaa 1020 acgcaccgct cgtcgccgtt acacccgtcg taaaaaccgc atctgctacc tgcaagaaat 1080 cttctctaac gaaatggcta aagtagatga cagctttttt caccgtctgg aagaatcatt 1140 tctggtggaa gaagataaaa agcacgaacg tcatccaatc ttcggcaaca ttgtggacga 1200 agtagcgtat cacgaaaaat acccgactat ctatcacctg cgcaaaaagc tggtcgattc 1260 gacggataag gccgatctgc gtctgatcta tctggcctta gcgcatatga ttaagttccg 1320 tggtcatttc ctgatcgaag gcgacctgaa tccagacaac agcgatgtag acaaactgtt 1380 catccagctg gtgcaaacct ataaccagct gtttgaagaa aacccaatta atgctagcgg 1440 tgttgacgcg aaagcgatct tgtccgcacg cctgtccaaa tcccgtcgtc tggaaaactt 1500 aattgcgcaa ctgccgggtg agaagaaaaa cggactgttc ggcaatctga tcgctcttag 1560 cttgggactg accccgaact tcaaaagcaa cttcgatctg gcagaggacg caaaacttca 1620 acttagcaaa gatacgtatg acgatgactt ggataactta ctggcccaga tcggagatca 1680 gtacgctgat ctgtttctgg cggcaaagaa cttatcagac gctattctcc tgtctgatat 1740 tcttcgtgtg aataccgaaa tcaccaaagc accgctttct gcatccatga ttaaacgcta 1800 tgacgaacat caccaagatc tgactcttct gaaagcgctg gtacggcaac aactgccgga 1860 gaagtacaag gagatcttct ttgaccaatc caaaaacggc tacgcgggtt atattgacgg 1920 gggtgcaagc caagaggagt tctacaaatt catcaagcca atcttagaaa aaatggatgg 1980 cacggaagaa ttacttgtta aactgaatcg tgaggatctg cttcgtaaac agcgtacctt 2040 cgacaacggt agcattccgc accagatcca cttaggtgaa ctgcacgcta tcctgcgtcg 2100 ccaagaggat ttttacccgt tcctgaaaga taatcgtgaa aaaatcgaaa aaatcctgac 2160 ctttcgtatc ccgtattatg tcggcccgct ggcgcgtggc aactcccgtt tcgcgtggat 2220 gactcgcaaa tccgaagaaa ctattacccc gtggaacttc gaggaagtgg ttgacaaagg 2280 cgcaagcgcc caatccttca tcgagcgcat gactaacttt gataaaaacc tgccgaacga 2340 aaaggtactg ccgaaacact cccttctgta cgaatacttc accgtgtaca acgagctgac 2400 taaagtaaag tatgtgactg agggcatgcg taaacctgca ttcctgagcg gtgaacagaa 2460 aaaagcaatt gttgatttac tgtttaaaac caaccgtaaa gtaaccgtta aacagctgaa 2520 agaggactac ttcaagaaaa tcgaatgctt cgactccgtc gagattagtg gagttgaaga 2580 tcgttttaat gcaagtttag gcacgtatca cgatttatta aagatcatta aagacaaaga 2640 tttcttggac aacgaagaaa atgaggacat cttagaggac atcgtcctga ccctgactct 2700 gttcgaagat cgtgaaatga ttgaagaacg cttgaagacg tatgctcacc tgtttgacga 2760 taaagtaatg aaacaactga aacgtcgccg ttatactggc tggggccgtc tgagccgtaa 2820 actgattaac ggtatccgtg acaaacagtc cggtaaaact attctggact tcctgaaatc 2880 tgacggcttc gcaaaccgta acttcatgca actgattcac gacgattccc tgaccttcaa 2940 agaggacatc cagaaagctc aggtttctgg tcaaggtgat tctctgcacg agcatatcgc 3000 caatttagca ggtagtccgg cgatcaaaaa aggtatcctg caaaccgtga aagtggtgga 3060 tgagcttgtg aaagttatgg gtcgtcacaa accggaaaac attgttatcg agatggctcg 3120 tgaaaaccaa acgacccaga agggacagaa aaactcccgc gaacgcatga aacgtatcga 3180 ggagggtatt aaagaacttg gctctcagat tctgaaagaa caccctgttg aaaataccca 3240 actgcaaaat gaaaaactgt acctgtacta cctgcaaaat ggtcgtgaca tgtatgtaga 3300 tcaggagctg gacatcaacc gcctctccga tacgacgtt gaccacattg ttccgcagtc 3360 ttttctgaaa gatgattcca ttgataacaa agtactcacc cgtagcgata aaaaccgtgg 3420 gaagagtgac aacgttccat cggaagaagt agttaagaaa atgaagaact attggcgtca 3480 actgcttaac gcgaaactga ttactcaacg taaatttgat aacctgacca aagctgaacg 3540 tggcggtttg tctgagctgg ataaggcggg ttttattaaa cgtcaactgg tagaaactcg 3600 ccagattaca aaacatgttg ctcagattct ggactctcgt atgaacacta aatacgatga 3660 aaatgacaaa ctgatccgcg aagttaaggt tattaccctg aaatctaagc tggtttccga 3720 cttccgtaaa gatttccaat tctataaagt gcgcgagatt aacaactatc accacgcgca 3780 cgacgcatat ctgaatgcag ttgttggcac ggcactgatc aaaaaatatc cgaaactgga 3840 aagcgaattt gtgtacggcg attataaagt ttacgacgtg cgcaaaatga tcgccaaatc 3900 tgaacaggaa attggcaaag caaccgctaa atactttttc tactcaaaca ttatgaattt 3960 cttcaaaacc gaaatcacct tagcgaatgg cgaaattcgt aaacgccctc tgatcgaaac 4020 caacggcgaa acgggtgaga tcgtgtggga caaaggtcgt gatttcgcta ctgtccgcaa 4080 agttctgtcc atgcctcaag taaacatcgt taaaaagact gaggtacaga ctggcggttt 4140 cagcaaggaa tccattctgc cgaaacgcaa ctccgacaaa ctgatcgcgc gtaagaaaga 4200 ctgggatccg aagaaatacg gtggcttcga ttctccaacc gtggcataca gcgttctggt 4260 agtcgccaaa gtcgaaaagg gtaaatcaaa aaaactgaaa tcagtgaaag aacttttagg 4320 catcaccatt atggaacgta gctctttcga aaaaaacccg attgacttcc tcgaagcgaa 4380 ggggtacaag gaagtaaaga aagatctgat tatcaaactg ccgaagtatt ccctgttcga 4440 actggaaaat ggtcgtaaac gtatgttagc gtctgcgggt gaactgcaaa aagggaacga 4500 attggccctt ccgtccaagt acgtgaactt cctgtatctg gcctcgcact acgagaaact 4560 gaaaggtagt ccggaagata atgagcagaa acagctgttc gtggaacagc acaaacacta 4620 tctggacgag attattgaac agatttctga gtttagcaaa cgcgtaattc tggcggacgc 4680 gaatctggat aaagtcctga gcgcctacaa taaacaccgt gataaaccga tccgtgaaca 4740 ggcagaaaac atcattcacc tgttcacgct gactaatctt ggtgctccgg cagccttcaa 4800 atacttcgac accacgatcg atcgtaaacg ttacacctcc actaaagaag tcttagatgc 4860 aactcttatt caccagagca tcactggcct gtatgaaact cgtattgatc tgagtcagtt 4920 gggcggtgac taataacgtt aaagtcagtt tcacctgttt tacgttaaaa cccgcttcgg 4980 cgggttttta cttttgggtt tagccgaacg ccccaaaaag cctcgctttc agcacctgtc 5040 gtttcctttc ttttcagagg gtattttaaa taaaaacatt aagttatgac gaagaagaac 5100 ggaaacgcct taaaccggaa aattttcata aatagcgaaa acccgcgagg tcgccgcccc 5160 gtaacctgtc ggatcaccgg aaaggacccg taaagtgata atgattatca tctacatatc 5220 acaacgtgcg taaagggact agtggatgtt tgatctcaaa aaaagcaccg actcggtgcc 5280 actttttcaa gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac 5340 ctgagaagac atctcctgcg cgtgctagca ttatacctag gactgagcta gctgtcagtc 5400 gggtagcacc agaagtctat agcatgtgca tacctttggt cgaaaaaaaa agcccgcact 5460 gtcaggtgcg ggcttttttc agtgtttcct tgccggatta cgccccgccc tgccactcat 5520 cgcagtattg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat 5580 gatgaacttg gatcgccagt ggcattaaca ccttgtcgcc ttgcgtataa tattttccca 5640 tagtgaaaac gggggcgaag aagttgtcca tatttgctac gtttaaatca aaactggtga 5700 aactcaccca cggattggca ctgacgaaaa acatattttc gataaaccct ttagggaaat 5760 atgctaagtt ttcaccgtaa cacgccacat cttgactata tatgtgtaga aactgccgga 5820 aatcgtcgtg gtattctgac cagagcgatg aaaacgtttc agtttgctca tggaaaacgg 5880 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa 5940 actccggatg tgcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt 6000 gcttattttt ctttacggtt tttaaaaagg ccgtaatatc cagctgaacg gtttggttat 6060 aggtgcactg agcaactgac tggaatgcct caaaatgttc tttacgatgc cattgactta 6120 tatcaactgt agtatatcca gtgatttttt tctccatttt agcttcctta gcttgcgaaa 6180 tctcgataac tcaaaaaata gtagtgatct tatttcatta tggtgaaagt tgtcttacgt 6240 gcaacatttt cgcaaaaagt tggcgcttta tcaacactgt ccctcctgtt cagctactga 6300 cggtactgcg gaactgacta aagtagtgcg taacggcaaa agcaccgccg gacatcagcg 6360 ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtaag tgaagtgctt 6420 catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat 6480 attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa 6540 tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag 6600 tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga 6660 aatctgacgc tcaaatcagt ggtggcgaaa cctgacagga ctataaagat accaggcgtt 6720 tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat 6780 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag 6840 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat 6900 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag 6960 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggata aggctaaact 7020 gaaaggacaa gttttggcga ctgcgctcct ccaagccagt tacctcggtt caaagagttg 7080 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa 7140 gagatacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat 7200 ttctagattt cagtgcaatt tatctcttca aatgtagcac 7240 <210> 4 <211> 2165 <212> DNA <213> Artificial Sequence <220> <223> GG2K (p502) <400> 4 ctgatgaggg tgtaagtgaa gtgcttcatg tggcaggaga aaaaaggctg catcggtgcg 60 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc 120 ggtcgttcga ctgtggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga 180 tgccaggaag atacttaaca gggaagtgag agggtcgcgg caaagccgtt tttccatagg 240 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacctg 300 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt 360 cctgcctttc ggtttgccgg tgtcattcct ctgttacggc cgagtttgtc tcattccacg 420 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc 480 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag 540 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga 600 agtcatgcgc cggataaggc taaactgaaa ggacaagttt tggcgactgc gctcctccaa 660 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca 720 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag 780 atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg 840 tagcaccggc gcgccgtgac caattattga aggccgctaa cgcggccttt ttttgtttct 900 ggtatcccga atggagcgac ttctccccaa aaagcctcgc tttcagcacc tgtcgtttcc 960 tttcttttca gagggtattt taaataaaaa cattaagtta tgacgaagaa gaacggaaac 1020 gccttaaacc ggaaaatttt cataaatagc gaaaacccgc gaggtcgccg ccccgtaacc 1080 tgtcggatca ccggaaagga cccgtaaagt gataatgatt atcatctaca tatcacaacg 1140 tgcgtaaagg gtaagtatga aggtcgtgta ctccgtaact tcgacccaca ttccttaaag 1200 agtgcatgtg catattttgt tatcaataaa aaaggccgcg atttgcggcc ttattgttcg 1260 tcttgccgga tacgccccg ccctgccact catcgcagta ttgttgtaat tcattaagca 1320 ttctgccgac atggaagcca tcacaaacgg catgatgaac ttggatcgcc agtggcatta 1380 acaccttgtc gccttgcgta taatattttc ccatagtgaa aacgggggcg aagaagttgt 1440 ccatatttgc tacgtttaaa tcaaaactgg tgaaactcac ccacggattg gcactgacga 1500 aaaacatatt ttcgataaac cctttaggga aatatgctaa gttttcaccg taacacgcca 1560 catcttgact atatatgtgt agaaactgcc ggaaatcgtc gtggtattct gaccagagcg 1620 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata 1680 tcaccagctc accgtctttc attgccatac gaaactccgg atgtgcattc atcaggcggg 1740 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtttttaaaa 1800 aggccgtaat atccagctga acggtttggt tataggtgca ctgagcaact gactggaatg 1860 cctcaaaatg ttctttacga tgccattgac ttatatcaac tgtagtatat ccagtgattt 1920 ttttctccat tttagcttcc ttagcttgcg aaatctcgat aactcaaaaa atagtagtga 1980 tcttatttca ttatggtgaa agttgtctta cgtgcaacat tttcgcaaaa agttggcgct 2040 ttatcaacac tgtcggaatg acaaatggtt ccaattattg aacacccttc ggggtgtttt 2100 tttgtttctg gtttcccgag gccggcctgc gctagcggag tgtatactgg cttactatgt 2160 tggca 2165 <210> 5 <211> 4073 <212> DNA <213> Artificial Sequence <220> <223> GG4K (p710) <400> 5 ttcgcaaaaa gttggcgctt tatcaacact gtcggaatga caaatggttc caattattga 60 acacccttcg gggtgttttt ttgtttctgg tttcccgagg ccggcctgcg ctagcggagt 120 gtatactggc ttactatgtt ggcactgatg agggtgtaag tgaagtgctt catgtggcag 180 gagaaaaaag gctgcatcgg tgcgtcagca gaatatgtga tacaggatat attccgcttc 240 ctcgctcact gactcgctac gctcggtcgt tcgactgtgg cgagcggaaa tggcttacga 300 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgaggggtc 360 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc 420 tcaaatcagt ggtggcgaaa cctgacagga ctataaagat accaggcgtt tccccctggc 480 ggctccctcg tgcgctctcc tgttcctgcc tttcggtttg ccggtgtcat tcctctgtta 540 cggccgagtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa 600 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta 660 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa 720 ttgatttaga ggagttagtc ttgaagtcat gcgccggata aggctaaact gaaaggacaa 780 gttttggcga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga 840 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc 900 gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt 960 cagtgcaatt tatctcttca aatgtagcac cggcgcgccg tgaccaatta ttgaaggccg 1020 ctaacgcggc ctttttttgt ttctggtatc ccgaatggag cgacttctcc ccaaaaagcc 1080 tcgctttcag cacctgtcgt ttcctttctt ttcagagggt attttaaata aaaacattaa 1140 gttatgacga agaagaacgg aaacgcctta aaccggaaaa ttttcataaa tagcgaaaac 1200 ccgcgaggtc gccgccccgt aacctgtcgg atcaccggaa aggacccgta aagtgataat 1260 gattatcatc tacatatcac aacgtgcgta aagggtaagt atgaaggtcg tgtactccat 1320 cgctaccaaa ttccagaaaa cagacgcttt cgagcgtctt ttttcgtttt ggtcacgacg 1380 tactgaaatc catgtcttct cagtcagatc tataataaag actttagcgc gtacagcaaa 1440 ggccgtccga atctgcacac cctttactgg aaagcattat ttgacgaacg taacctgcaa 1500 gatgtggtgt ataaactgaa cggtgaggcg gaacttttct accgtaaaca gagtatcccg 1560 aagaaaatca cgcatccggc aaaagaagct attgccaaca aaaacaaaga caacccgaag 1620 aaagaatcag tattcgaata tgacctgatc aaagataaac gtttcaccga agataagttc 1680 tttttccact gtccgattac catcaacttc aaatctagcg gtgcgaacaa gttcaacgat 1740 gaaattaact tattactgaa agagaaagct aatgacgtac acatcttatc tattgatcgc 1800 ggtgaacgtc atttagcata ctatacactg gtagatggta aaggtaatat tattaaacag 1860 gatactttca atattatcgg taatgaccgt atgaaaacca actatcacga taagctggcg 1920 gcgatcgaaa aagatcgtga ttctgcgcgt aaagattgga agaaaattaa caatatcaaa 1980 gaaatgaaag aaggctatct gagccaagtg gtgcacgaga tcgcaaaact ggtgattgaa 2040 tataacgcta tcgtggtttt cgaagatctg aactttggtt ttaaacgtgg tcgcttcaaa 2100 gtagaaaaac aggtgtacca aaaactggaa aaaatgctga ttgaaaaact gaactatctg 2160 gtttttaaag acaacgaatt tgacaaaacg ggtggcgtac tccgtgccta tcagctgacc 2220 gctccgttcg aaacgttcaa gaaaatgggt aaacaaacgg ggattatcta ttatgtgcca 2280 gctggtttca cctccaagat ttgtccagtt acgggcttcg ttaaccagct gtacccgaaa 2340 tacgagagcg ttagcaaatc tcaagaattt ttcagcaaat tcgacaagat ctgctataat 2400 ctggataaag gctatttcga gttcagcttc gattacaaaa acttcggcga taaagcggct 2460 aaaggtaagt ggactattgc tagctttggt agccgtctga ttaactttcg caactccgac 2520 aaaaaccata attgggacac gcgtgaagtg tatccgacca aagaactgga aaaattactg 2580 aaagactatt ccatcgaata tggtcatggg gagtgcatta aagcggcgat ttgcggtgaa 2640 tccgataaga aatttttcgc caaactgacc agcgtgctta acaccattct gcaaatgcgt 2700 aattctaaaa cgggtacgga gctggactac ctgatttctc cggtagccga cgttaacggc 2760 aacttcttcg attctcgtca agcaccgaaa aatatgccac aagacgcgga tgccaacggt 2820 gcataccata tcggcttaaa aggcttaatg ttattaggcc gtatcaagaa taatcaggag 2880 ggcaagaaat taaatctggt tatcaaaaac gaagaatact tcgagttcgt tcagaatcgt 2940 aacaattaat gtatgcttaa gcagctcggt accaaagacg aacaataaga cgctgaaaag 3000 cgtctttttt cgttttggtc ctgttgcggc gcgatagtgt gaacatgcta tagacttctg 3060 gtgctacccg actgacagct agctcagtcc taggtataat gctagcaatt tctactgttg 3120 tagatagaag agcttcatcg ctcttcatcg agacgaacaa taaggcctcc ctaacggggg 3180 gcctttttta ttgataacaa aagtaacttc gagcttgtct acctccgtaa attgcaccca 3240 cattccttaa agagtgcatg tgcatatttt gttatcaata aaaaaggccg cgatttgcgg 3300 ccttattgtt cgtcttgccg gattacgccc cgccctgcca ctcatcgcag tattgttgta 3360 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acttggatcg 3420 ccagtggcat taacaccttg tcgccttgcg tataatattt tcccatagtg aaaacggggg 3480 cgaagaagtt gtccatattt gctacgttta aatcaaaact ggtgaaactc acccacggat 3540 tggcactgac gaaaaacata ttttcgataa accctttagg gaaatatgct aagttttcac 3600 cgtaacacgc cacatcttga ctatatatgt gtagaaactg ccggaaatcg tcgtggtatt 3660 ctgaccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa 3720 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaactcc ggatgtgcat 3780 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta 3840 cggtttttaa aaaggccgta atatccagct gaacggtttg gttataggtg cactgagcaa 3900 ctgactggaa tgcctcaaaa tgttctttac gatgccattg acttatatca actgtagtat 3960 atccagtgat ttttttctcc attttagctt ccttagcttg cgaaatctcg ataactcaaa 4020 aaatagtagt gatcttattt cattatggtg aaagttgtct tacgtgcaac att 4073 <210> 6 <211> 6491 <212> DNA <213> Artificial Sequence <220> <223> GG6K (p504) <400> 6 gcccgcagaa agggtatgaa aaattcgaat ttaatataga ggactgccgt aagttcatcg 60 acttctataa acagagcatt tccaaacatc cggaatggaa agacttcggc ttccgtttct 120 ctgacactca gcgctataat agcatcgacg agttctaccg cgaagtggag aatcagggct 180 ataaactgac cttcgagaac attagtgagt cgtacatcga ctccgttgtg aatcagggta 240 aactgtacct gtttcagatc tataataaag actttagcgc gtacagcaaa ggccgtccga 300 atctgcacac cctttactgg aaagcattat ttgacgaacg taacctgcaa gatgtggtgt 360 ataaactgaa cggtgaggcg gaacttttct accgtaaaca gagtatcccg aagaaaatca 420 cgcatccggc aaaagaagct attgccaaca aaaacaaaga caacccgaag aaagaatcag 480 tattcgaata tgacctgatc aaagataaac gtttcaccga agataagttc tttttccact 540 gtccgattac catcaacttc aaatctagcg gtgcgaacaa gttcaacgat gaaattaact 600 tattactgaa agagaaagct aatgacgtac acatcttatc tattgatcgc ggtgaacgtc 660 atttagcata ctatacactg gtagatggta aaggtaatat tattaaacag gatactttca 720 atattatcgg taatgaccgt atgaaaacca actatcacga taagctggcg gcgatcgaaa 780 aagatcgtga ttctgcgcgt aaagattgga agaaaattaa caatatcaaa gaaatgaaag 840 aaggctatct gagccaagtg gtgcacgaga tcgcaaaact ggtgattgaa tataacgcta 900 tcgtggtttt cgaagatctg aactttggtt ttaaacgtgg tcgcttcaaa gtagaaaaac 960 aggtgtacca aaaactggaa aaaatgctga ttgaaaaact gaactatctg gtttttaaag 1020 acaacgaatt tgacaaaacg ggtggcgtac tccgtgccta tcagctgacc gctccgttcg 1080 aaacgttcaa gaaaatgggt aaacaaacgg ggattatcta ttatgtgcca gctggtttca 1140 cctccaagat ttgtccagtt acgggcttcg ttaaccagct gtacccgaaa tacgagagcg 1200 ttagcaaatc tcaagaattt ttcagcaaat tcgacaagat ctgctataat ctggataaag 1260 gctatttcga gttcagcttc gattacaaaa acttcggcga taaagcggct aaaggtaagt 1320 ggactattgc tagctttggt agccgtctga ttaactttcg caactccgac aaaaaccata 1380 attgggacac gcgtgaagtg tatccgacca aagaactgga aaaattactg aaagactatt 1440 ccatcgaata tggtcatggg gagtgcatta aagcggcgat ttgcggtgaa tccgataaga 1500 aatttttcgc caaactgacc agcgtgctta acaccattct gcaaatgcgt aattctaaaa 1560 cgggtacgga gctggactac ctgatttctc cggtagccga cgttaacggc aacttcttcg 1620 attctcgtca agcaccgaaa aatatgccac aagacgcgga tgccaacggt gcataccata 1680 tcggcttaaa aggcttaatg ttattaggcc gtatcaagaa taatcaggag ggcaagaaat 1740 taaatctggt tatcaaaaac gaagaatact tcgagttcgt tcagaatcgt aacaattaat 1800 gtatgcttaa gcagctcggt accaaagacg aacaataaga cgctgaaaag cgtctttttt 1860 cgttttggtc ctgttgcggc gcgatagtgt gaacatgcta tagacttctg gtgctacccg 1920 actgacagct agctcagtcc taggtataat gctagcaatt tctactgttg tagatagaag 1980 agcttcatcg ctcttcatcg agacgaacaa taaggcctcc ctaacggggg gcctttttta 2040 ttgataacaa aagtaacttc gagcttgtct acctccgtaa attgcaccca cattccttaa 2100 agagtgcatg tgcatatttt gttatcaata aaaaaggccg cgatttgcgg ccttattgtt 2160 cgtcttgccg gattacgccc cgccctgcca ctcatcgcag tattgttgta attcattaag 2220 cattctgccg acatggaagc catcacaaac ggcatgatga acttggatcg ccagtggcat 2280 taacaccttg tcgccttgcg tataatattt tcccatagtg aaaacggggg cgaagaagtt 2340 gtccatattt gctacgttta aatcaaaact ggtgaaactc acccacggat tggcactgac 2400 gaaaaacata ttttcgataa accctttagg gaaatatgct aagttttcac cgtaacacgc 2460 cacatcttga ctatatatgt gtagaaactg ccggaaatcg tcgtggtatt ctgaccagag 2520 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca 2580 tatcaccagc tcaccgtctt tcattgccat acgaaactcc ggatgtgcat tcatcaggcg 2640 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtttttaa 2700 aaaggccgta atatccagct gaacggtttg gttataggtg cactgagcaa ctgactggaa 2760 tgcctcaaaa tgttctttac gatgccattg acttatatca actgtagtat atccagtgat 2820 ttttttctcc attttagctt ccttagcttg cgaaatctcg ataactcaaa aaatagtagt 2880 gatcttattt cattatggtg aaagttgtct tacgtgcaac attttcgcaa aaagttggcg 2940 ctttatcaac actgtcggaa tgacaaatgg ttccaattat tgaacaccct tcggggtgtt 3000 tttttgtttc tggtttcccg aggccggcct gcgctagcgg agtgtatact ggcttactat 3060 gttggcactg atgagggtgt aagtgaagtg cttcatgtgg caggagaaaa aaggctgcat 3120 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc 3180 tacgctcggt cgttcgactg tggcgagcgg aaatggctta cgaacggggc ggagatttcc 3240 tggaagatgc caggaagata cttaacaggg aagtgagagg gtcgcggcaa agccgttttt 3300 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg 3360 aaacctgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc 3420 tcctgttcct gcctttcggt ttgccggtgt cattcctctg ttacggccga gtttgtctca 3480 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg 3540 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 3600 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta 3660 gtcttgaagt catgcgccgg ataaggctaa actgaaagga caagttttgg cgactgcgct 3720 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg 3780 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct 3840 caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct 3900 tcaaatgtag caccggcgcg ccgtgaccaa ttattgaagg ccgctaacgc ggcctttttt 3960 tgtttctggt atcccgaatg gagcgacttc tccccaaaaa gcctcgcttt cagcacctgt 4020 cgtttccttt cttttcagag ggtattttaa ataaaaacat taagttatga cgaagaagaa 4080 cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa aacccgcgag gtcgccgccc 4140 cgtaacctgt cggatcaccg gaaaggaccc gtaaagtgat aatgattatc atctacatat 4200 cacaacgtgc gtaaagggta agtatgaagg tcgtgtactc catcgctacc aaattccaga 4260 aaacagacgc tttcgagcgt cttttttcgt tttggtcacg acgtactgaa tctgattcgt 4320 taccaattga catgatacga aacgtaccgt atcgttaagg ttactagaag agcgattggg 4380 ctcttcaatg tccatctatc aggagtttgt taacaagtat tccctgtcta aaaccctgcg 4440 ttttgaactg atcccgcagg gcaaaacttt ggaaaacatt aaagcgcgtg gcctgattct 4500 ggatgacgaa aaacgtgcaa aggattacaa gaaagctaaa cagatcatcg acaaatatca 4560 ccagttcttt atcgaagaaa ttctgtcgtc ggtgtgcatc agtgaggatc tgttacagaa 4620 ttatctgat gtatacttta aacttaaaaa gtccgatgac gataatctgc aaaaagattt 4680 caagtcagcc aaagatacca tcaagaaaca gatctcagaa tatattaaag atagcgaaaa 4740 gttcaaaaac ctgtttaacc aaaacctcat tgatgctaag aaaggccaag aatctgacct 4800 gatcttatgg ctgaaacaga gcaaagataa cggcattgaa ctgttcaaag ctaatagcga 4860 catcaccgat attgatgaag cgctcgaaat catcaagtct ttcaaaggct ggacgacgta 4920 tttcaaaggt tttcatgaaa accgtaagaa tgtatattcg agcaacgata ttccgacctc 4980 tattatttat cgtatcgtgg acgacaacct gccgaagttt ctggaaaaca aagcgaaata 5040 tgaatctctg aaagacaaag caccggaagc tattaactat gaacagatca agaaagatct 5100 ggcggaagaa ctgaccttcg acatcgacta taaaacctcc gaagttaacc agcgtgtttt 5160 ctcactggac gaggttttcg aaatcgctaa tttcaacaat tacctgaatc aatctggcat 5220 caccaaattc aacaccatta ttggtggcaa atttgttaac ggcgaaaaca ccaagcgtaa 5280 gggcatcaac gaatacatta acctgtatag ccaacaaatc aacgacaaaa ccctgaaaaa 5340 gtataaaatg tccgttctgt ttaaacagat tttatcggac accgaatcta aatccttcgt 5400 aattgataaa ctggaagatg atagcgacgt tgtcaccacg atgcagagct tttatgagca 5460 gattgcggcg ttcaaaaccg tggaagagaa atctattaaa gaaactctgt ccctgctctt 5520 tgacgacctc aaagcgcaga aactagatct gtctaagatt tactttaaaa acgacaaatc 5580 tctgaccgat ctcagtcaac aagttttcga tgactatagc gtgatcggca cggcagtttt 5640 ggaatacatc acccaacaaa tcgcgccgaa aaatctggac aacccgtcca agaaggaaca 5700 ggaactgatt gcaaagaaaa cagaaaaagc taaatacctg agcttagaaa ctatcaaact 5760 ggcacttgag gaatttaata aacatcgtga tattgataaa cagtgtcgtt ttgaggaaat 5820 tctggcgaac tttgcggcaa tcccgatgat cttcgacgaa attgctcaaa acaaagacaa 5880 tctggcgcag atctctatca agtaccagaa tcagggtaag aaagatctgc ttcaagcatc 5940 tgcggaggac gatgtgaaag caattaaaga cttattagat cagacgaata acttattaca 6000 caagctcaaa atcttccaca tcagccagag cgaggacaag gcgaacattc tggataaaga 6060 tgaacacttc tatctggtgt tcgaagaatg ttacttcgaa ctggcaaaca tcgtccctct 6120 ctacaataaa atccgcaact acatcacgca gaagccttac tctgacgaga aattcaaact 6180 gaacttcgaa aacagcacgc tggcgaacgg ctgggataag aacaaagagc cggacaacac 6240 cgcaatcctg ttcatcaaag acgacaaata ctatctgggc gtaatgaaca agaagaacaa 6300 caagatcttc gacgataaag cgatcaaaga aaacaagggt gaaggctata agaaaatcgt 6360 gtacaagctc ctgccgggtg cgaataaaat gttaccgaaa gtgttctttt ccgcgaaaag 6420 catcaaattc tacaacccgt ctgaggatat tctgcgcatc cgcaatcata gcacgcacac 6480 taaaaacggt a 6491 <210> 7 <211> 7980 <212> DNA <213> Artificial Sequence <220> <223> GG8K (p711) <400> 7 actgtcggaa tgacaaatgg ttccaattat tgaacaccct tcggggtgtt tttttgtttc 60 tggtttcccg aggccggcct gcgctagcgg agtgtatact ggcttactat gttggcactg 120 atgagggtgt aagtgaagtg cttcatgtgg caggagaaaa aaggctgcat cggtgcgtca 180 gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt 240 cgttcgactg tggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc 300 caggaagata cttaacaggg aagtgagagg gtcgcggcaa agccgttttt ccataggctc 360 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacctgaca 420 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct 480 gcctttcggt ttgccggtgt cattcctctg ttacggccga gtttgtctca ttccacgcct 540 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt 600 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca 660 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt 720 catgcgccgg ataaggctaa actgaaagga caagttttgg cgactgcgct cctccaagcc 780 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg 840 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc 900 atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag 960 caccggcgcg ccgtgaccaa ttattgaagg ccgctaacgc ggcctttttt tgtttctggt 1020 atcccgaatg gagcgacttc tccccaaaaa gcctcgcttt cagcacctgt cgtttccttt 1080 cttttcagag ggtattttaa ataaaaacat taagttatga cgaagaagaa cggaaacgcc 1140 ttaaaccgga aaattttcat aaatagcgaa aacccgcgag gtcgccgccc cgtaacctgt 1200 cggatcaccg gaaaggaccc gtaaagtgat aatgattatc atctacatat cacaacgtgc 1260 gtaaagggta agtatgaagg tcgtgtactc catcgctacc aaattccaga aaacagacgc 1320 tttcgagcgt cttttttcgt tttggtcacg acgtactgaa tctgattcgt taccaattga 1380 catgatacga aacgtaccgt atcgttaagg ttactagaag agcgattggg ctcttcaatg 1440 tccatctatc aggagtttgt taacaagtat tccctgtcta aaaccctgcg ttttgaactg 1500 atcccgcagg gcaaaacttt ggaaaacatt aaagcgcgtg gcctgattct ggatgacgaa 1560 aaacgtgcaa aggattacaa gaaagctaaa cagatcatcg acaaatatca ccagttcttt 1620 atcgaagaaa ttctgtcgtc ggtgtgcatc agtgaggatc tgttacagaa ttattctgat 1680 gtatacttta aacttaaaaa gtccgatgac gataatctgc aaaaagattt caagtcagcc 1740 aaagatacca tcaagaaaca gatctcagaa tatattaaag atagcgaaaa gttcaaaaac 1800 ctgtttaacc aaaacctcat tgatgctaag aaaggccaag aatctgacct gatcttatgg 1860 ctgaaacaga gcaaagataa cggcattgaa ctgttcaaag ctaatagcga catcaccgat 1920 attgatgaag cgctcgaaat catcaagtct ttcaaaggct ggacgacgta tttcaaaggt 1980 tttcatgaaa accgtaagaa tgtatattcg agcaacgata ttccgacctc tattatttat 2040 cgtatcgtgg acgacaacct gccgaagttt ctggaaaaca aagcgaaata tgaatctctg 2100 aaagacaaag caccggaagc tattaactat gaacagatca agaaagatct ggcggaagaa 2160 ctgaccttcg acatcgacta taaaacctcc gaagttaacc agcgtgtttt ctcactggac 2220 gaggttttcg aaatcgctaa tttcaacaat tacctgaatc aatctggcat caccaaattc 2280 aacaccatta ttggtggcaa atttgttaac ggcgaaaaca ccaagcgtaa gggcatcaac 2340 gaatacatta acctgtatag ccaacaaatc aacgacaaaa ccctgaaaaa gtataaaatg 2400 tccgttctgt ttaaacagat tttatcggac accgaatcta aatccttcgt aattgataaa 2460 ctggaagatg atagcgacgt tgtcaccacg atgcagagct tttatgagca gattgcggcg 2520 ttcaaaaccg tggaagagaa atctatttaaa gaaactctgt ccctgctctt tgacgacctc 2580 aaagcgcaga aactagatct gtctaagatt tactttaaaa acgacaaatc tctgaccgat 2640 ctcagtcaac aagttttcga tgactatagc gtgatcggca cggcagtttt ggaatacatc 2700 acccaacaaa tcgcgccgaa aaatctggac aacccgtcca agaaggaaca ggaactgatt 2760 gcaaagaaaa cagaaaaagc taaatacctg agcttagaaa ctatcaaact ggcacttgag 2820 gaatttaata aacatcgtga tattgataaa cagtgtcgtt ttgaggaaat tctggcgaac 2880 tttgcggcaa tcccgatgat cttcgacgaa attgctcaaa acaaagacaa tctggcgcag 2940 atctctatca agtaccagaa tcagggtaag aaagatctgc ttcaagcatc tgcggaggac 3000 gatgtgaaag caattaaaga cttattagat cagacgaata acttattaca caagctcaaa 3060 atcttccaca tcagccagag cgaggacaag gcgaacattc tggataaaga tgaacacttc 3120 tatctggtgt tcgaagaatg ttacttcgaa ctggcaaaca tcgtccctct ctacaataaa 3180 atccgcaact acatcacgca gaagccttac tctgacgaga aattcaaact gaacttcgaa 3240 aacagcacgc tggcgaacgg ctgggataag aacaaagagc cggacaacac cgcaatcctg 3300 ttcatcaaag acgacaaata ctatctgggc gtaatgaaca agaagaacaa caagatcttc 3360 gacgataaag cgatcaaaga aaacaagggt gaaggctata agaaaatcgt gtacaagctc 3420 ctgccgggtg cgaataaaat gttaccgaaa gtgttctttt ccgcgaaaag catcaaattc 3480 tacaacccgt ctgaggatat tctgcgcatc cgcaatcata gcacgcacac taaaaacggt 3540 agccgcaga aagggtatga aaaattcgaa tttaatatag aggactgccg taagttcatc 3600 gacttctata aacagagcat ttccaaacat ccggaatgga aagacttcgg cttccgtttc 3660 tctgacactc agcgctataa tagcatcgac gagttctacc gcgaagtgga gaatcagggc 3720 tataaactga ccttcgagaa cattagtgag tcgtacatcg actccgttgt gaatcagggt 3780 aaactgtacc tgtttcagat ctataataaa gactttagcg cgtacagcaa aggccgtccg 3840 aatctgcaca ccctttactg gaaagcatta tttgacgaac gtaacctgca agatgtggtg 3900 tataaactga acggtgaggc ggaacttttc taccgtaaac agagtatccc gaagaaaatc 3960 acgcatccgg caaaagaagc tattgccaac aaaaacaaag acaacccgaa gaaagaatca 4020 gtattcgaat atgacctgat caaagataaa cgtttcaccg aagataagtt ctttttccac 4080 tgtccgatta ccatcaactt caaatctagc ggtgcgaaca agttcaacga tgaaattaac 4140 ttattactga aagagaaagc taatgacgta cacatcttat ctattgatcg cggtgaacgt 4200 catttagcat actatacact ggtagatggt aaaggtaata ttattaaaca ggatactttc 4260 aatattatcg gtaatgaccg tatgaaaacc aactatcacg ataagctggc ggcgatcgaa 4320 aaagatcgtg attctgcgcg taaagattgg aagaaaatta acaatatcaa agaaatgaaa 4380 gaaggctatc tgagccaagt ggtgcacgag atcgcaaaac tggtgattga atataacgct 4440 atcgtggttt tcgaagatct gaactttggt tttaaacgtg gtcgcttcaa agtagaaaaa 4500 caggtgtacc aaaaactgga aaaaatgctg attgaaaaac tgaactatct ggtttttaaa 4560 gacaacgaat ttgacaaaac gggtggcgta ctccgtgcct atcagctgac cgctccgttc 4620 gaaacgttca agaaaatggg taaacaaacg gggattatct attatgtgcc agctggtttc 4680 acctccaaga tttgtccagt tacgggcttc gttaaccagc tgtacccgaa atacgagagc 4740 gttagcaaat ctcaagaatt tttcagcaaa ttcgacaaga tctgctataa tctggataaa 4800 ggctatttcg agttcagctt cgattacaaa aacttcggcg ataaagcggc taaaggtaag 4860 tggactattg ctagctttgg tagccgtctg attaactttc gcaactccga caaaaaccat 4920 aattgggaca cgcgtgaagt gtatccgacc aaagaactgg aaaaattact gaaagactat 4980 tccatcgaat atggtcatgg ggagtgcatt aaagcggcga tttgcggtga atccgataag 5040 aaatttttcg ccaaactgac cagcgtgctt aacaccattc tgcaaatgcg taattctaaa 5100 acgggtacgg agctggacta cctgatttct ccggtagccg acgttaacgg caacttcttc 5160 gattctcgtc aagcaccgaa aaatatgcca caagacgcgg atgccaacgg tgcataccat 5220 atcggcttaa aaggcttaat gttattaggc cgtatcaaga ataatcagga gggcaagaaa 5280 ttaaatctgg ttatcaaaaa cgaagaatac ttcgagttcg ttcagaatcg taacaattaa 5340 tgtatgctta agcagctcgg taccaaagac gaacaataag acgctgaaaa gcgtcttttt 5400 tcgttttggt cctgttgcgg cgcgatagtg tgaacatgct atagacttct ggtgctaccc 5460 gactgacagc tagctcagtc ctaggtataa tgctagcaat ttctactgtt gtagatagaa 5520 gagcttcatc gctcttcatc gagacgaaca ataaggcctc cctaacgggg ggcctttttt 5580 attgataaca aaagtaactt cgagcttgtc tacctcctag cactttatag ctagctcagc 5640 ccttggtaca atgctagcgt tttcattaaa gaggagaaag gaagccatga gtaaaggtga 5700 ggaattattt actggtgttg ttccgatctt agttgaactg gacggcgatg ttaacggtca 5760 taaattcagt gttcgtggtg aaggtgaagg tgatgcaacc aacggtaagc tgaccctgaa 5820 attcatctgc actactggaa aattaccagt accgtggcct actctggtga ctaccctgac 5880 ctatggtgtt cagtgttttt ctcgttaccc tgaccacatg aagcaacatg atttcttcaa 5940 atctgcaatg ccggaaggtt atgtacagga gcgcaccatt tctttcaaag acgatggcac 6000 gtataaaacc cgtgcagagg ttaaatttga aggtgacact ctggtgaatc gtattgaact 6060 gaaaggcatt gatttcaaag aggacggcaa tattttaggc cacaaactgg aatataactt 6120 caactcccat aacgtttaca tcaccgcaga caaacagaag aacggtatca aagctaactt 6180 caaaattcgc cataacgttg aagatggtag cgtacagctg gcggatcatt accaacagaa 6240 cactccgatt ggagatgctc ctgttttact gccggataac cactacctgt ccacccagtc 6300 taaactgtcg aaggatccga acgaaaagcg cgaccacatg gtgttattag agttcgttac 6360 cgctagtggt atcacgcacg gtatggatga actctacaaa taagacgaac aataagggga 6420 gcgggaaacc gctccccttt tttattgata acaaaagtaa attgcacgct gatagtctcc 6480 caattgcgaa ggaccaaaac gaaaaaacac cctttcgggt gtcttttctg gaatttggta 6540 ccgagtacta ggtatcgtgt aagtagcgaa ggcccgtacg cgagataaac tgctaggcaa 6600 ccgcgactct acgactggtg ctcgatttaa tttcgctgac gtaaagaaat tatcggcagt 6660 gcgtcaactg ccgtatcttt atcttaatta ggtagttgga caagcccttg aaagaaatag 6720 caagagcctg cctctctatt gaagtcacgg cgaaagtcgg gtagaaatca aagaaagcag 6780 aaattaaatc ggagtaacac taaggtggga taactccgta actgactacg cctttctcta 6840 gactttactt gaccagatac actgtctttg acacgttgaa ggattagagc aatcaaatcc 6900 aagactggct aagcacgaag caactcttga gtgttaaaaa gttatctcct gtattcggga 6960 agcgggtact agaagattgc agggactccg acgttaagta aattacaaag taataagtat 7020 cgttcaggat cacgttaccg caataagaag cgagaataat ataatttccg aagtgcttac 7080 cccagtagtg actattccta taacccttct gagtgtccgg cattccttaa agagtgcatg 7140 tgcatatttt gttatcaata aaaaaggccg cgatttgcgg ccttattgtt cgtcttgccg 7200 gattacgccc cgccctgcca ctcatcgcag tattgttgta attcattaag cattctgccg 7260 acatggaagc catcacaaac ggcatgatga acttggatcg ccagtggcat taacaccttg 7320 tcgccttgcg tataatattt tcccatagtg aaaacggggg cgaagaagtt gtccatattt 7380 gctacgttta aatcaaaact ggtgaaactc acccacggat tggcactgac gaaaaacata 7440 ttttcgataa accctttagg gaaatatgct aagttttcac cgtaacacgc cacatcttga 7500 ctatatatgt gtagaaactg ccggaaatcg tcgtggtatt ctgaccagag cgatgaaaac 7560 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc 7620 tcaccgtctt tcattgccat acgaaactcc ggatgtgcat tcatcaggcg ggcaagaatg 7680 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtttttaa aaaggccgta 7740 atatccagct gaacggtttg gttataggtg cactgagcaa ctgactggaa tgcctcaaaa 7800 tgttctttac gatgccattg acttatatca actgtagtat atccagtgat ttttttctcc 7860 attttagctt ccttagcttg cgaaatctcg ataactcaaa aaatagtagt gatcttattt 7920 cattatggtg aaagttgtct tacgtgcaac attttcgcaa aaagttggcg ctttatcaac 7980 7980 <210> 8 <211> 12262 <212> DNA <213> Artificial Sequence <220> <223> GG12K (p499) <400> 8 actgtcggaa tgacaaatgg ttccaattat tgaacaccct tcggggtgtt tttttgtttc 60 tggtttcccg aggccggcct gcgctagcgg agtgtatact ggcttactat gttggcactg 120 atgagggtgt aagtgaagtg cttcatgtgg caggagaaaa aaggctgcat cggtgcgtca 180 gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt 240 cgttcgactg tggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc 300 caggaagata cttaacaggg aagtgagagg gtcgcggcaa agccgttttt ccataggctc 360 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacctgaca 420 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct 480 gcctttcggt ttgccggtgt cattcctctg ttacggccga gtttgtctca ttccacgcct 540 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt 600 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca 660 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt 720 catgcgccgg ataaggctaa actgaaagga caagttttgg cgactgcgct cctccaagcc 780 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg 840 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc 900 atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag 960 caccggcgcg ccgtgaccaa ttattgaagg ccgctaacgc ggcctttttt tgtttctggt 1020 atcccgaatg gagcgacttc tccccaaaaa gcctcgcttt cagcacctgt cgtttccttt 1080 cttttcagag ggtattttaa ataaaaacat taagttatga cgaagaagaa cggaaacgcc 1140 ttaaaccgga aaattttcat aaatagcgaa aacccgcgag gtcgccgccc cgtaacctgt 1200 cggatcaccg gaaaggaccc gtaaagtgat aatgattatc atctacatat cacaacgtgc 1260 gtaaagggta agtatgaagg tcgtgtactc catcgctacc aaattccaga aaacagacgc 1320 tttcgagcgt cttttttcgt tttggtcacg acgtactgaa tctgattcgt taccaattga 1380 catgatacga aacgtaccgt atcgttaagg ttactagaag agcgattggg ctcttcaatg 1440 tccatctatc aggagtttgt taacaagtat tccctgtcta aaaccctgcg ttttgaactg 1500 atcccgcagg gcaaaacttt ggaaaacatt aaagcgcgtg gcctgattct ggatgacgaa 1560 aaacgtgcaa aggattacaa gaaagctaaa cagatcatcg acaaatatca ccagttcttt 1620 atcgaagaaa ttctgtcgtc ggtgtgcatc agtgaggatc tgttacagaa ttattctgat 1680 gtatacttta aacttaaaaa gtccgatgac gataatctgc aaaaagattt caagtcagcc 1740 aaagatacca tcaagaaaca gatctcagaa tatattaaag atagcgaaaa gttcaaaaac 1800 ctgtttaacc aaaacctcat tgatgctaag aaaggccaag aatctgacct gatcttatgg 1860 ctgaaacaga gcaaagataa cggcattgaa ctgttcaaag ctaatagcga catcaccgat 1920 attgatgaag cgctcgaaat catcaagtct ttcaaaggct ggacgacgta tttcaaaggt 1980 tttcatgaaa accgtaagaa tgtatattcg agcaacgata ttccgacctc tattatttat 2040 cgtatcgtgg acgacaacct gccgaagttt ctggaaaaca aagcgaaata tgaatctctg 2100 aaagacaaag caccggaagc tattaactat gaacagatca agaaagatct ggcggaagaa 2160 ctgaccttcg acatcgacta taaaacctcc gaagttaacc agcgtgtttt ctcactggac 2220 gaggttttcg aaatcgctaa tttcaacaat tacctgaatc aatctggcat caccaaattc 2280 aacaccatta ttggtggcaa atttgttaac ggcgaaaaca ccaagcgtaa gggcatcaac 2340 gaatacatta acctgtatag ccaacaaatc aacgacaaaa ccctgaaaaa gtataaaatg 2400 tccgttctgt ttaaacagat tttatcggac accgaatcta aatccttcgt aattgataaa 2460 ctggaagatg atagcgacgt tgtcaccacg atgcagagct tttatgagca gattgcggcg 2520 ttcaaaaccg tggaagagaa atctatttaaa gaaactctgt ccctgctctt tgacgacctc 2580 aaagcgcaga aactagatct gtctaagatt tactttaaaa acgacaaatc tctgaccgat 2640 ctcagtcaac aagttttcga tgactatagc gtgatcggca cggcagtttt ggaatacatc 2700 acccaacaaa tcgcgccgaa aaatctggac aacccgtcca agaaggaaca ggaactgatt 2760 gcaaagaaaa cagaaaaagc taaatacctg agcttagaaa ctatcaaact ggcacttgag 2820 gaatttaata aacatcgtga tattgataaa cagtgtcgtt ttgaggaaat tctggcgaac 2880 tttgcggcaa tcccgatgat cttcgacgaa attgctcaaa acaaagacaa tctggcgcag 2940 atctctatca agtaccagaa tcagggtaag aaagatctgc ttcaagcatc tgcggaggac 3000 gatgtgaaag caattaaaga cttattagat cagacgaata acttattaca caagctcaaa 3060 atcttccaca tcagccagag cgaggacaag gcgaacattc tggataaaga tgaacacttc 3120 tatctggtgt tcgaagaatg ttacttcgaa ctggcaaaca tcgtccctct ctacaataaa 3180 atccgcaact acatcacgca gaagccttac tctgacgaga aattcaaact gaacttcgaa 3240 aacagcacgc tggcgaacgg ctgggataag aacaaagagc cggacaacac cgcaatcctg 3300 ttcatcaaag acgacaaata ctatctgggc gtaatgaaca agaagaacaa caagatcttc 3360 gacgataaag cgatcaaaga aaacaagggt gaaggctata agaaaatcgt gtacaagctc 3420 ctgccgggtg cgaataaaat gttaccgaaa gtgttctttt ccgcgaaaag catcaaattc 3480 tacaacccgt ctgaggatat tctgcgcatc cgcaatcata gcacgcacac taaaaacggt 3540 agccgcaga aagggtatga aaaattcgaa tttaatatag aggactgccg taagttcatc 3600 gacttctata aacagagcat ttccaaacat ccggaatgga aagacttcgg cttccgtttc 3660 tctgacactc agcgctataa tagcatcgac gagttctacc gcgaagtgga gaatcagggc 3720 tataaactga ccttcgagaa cattagtgag tcgtacatcg actccgttgt gaatcagggt 3780 aaactgtacc tgtttcagat ctataataaa gactttagcg cgtacagcaa aggccgtccg 3840 aatctgcaca ccctttactg gaaagcatta tttgacgaac gtaacctgca agatgtggtg 3900 tataaactga acggtgaggc ggaacttttc taccgtaaac agagtatccc gaagaaaatc 3960 acgcatccgg caaaagaagc tattgccaac aaaaacaaag acaacccgaa gaaagaatca 4020 gtattcgaat atgacctgat caaagataaa cgtttcaccg aagataagtt ctttttccac 4080 tgtccgatta ccatcaactt caaatctagc ggtgcgaaca agttcaacga tgaaattaac 4140 ttattactga aagagaaagc taatgacgta cacatcttat ctattgatcg cggtgaacgt 4200 catttagcat actatacact ggtagatggt aaaggtaata ttattaaaca ggatactttc 4260 aatattatcg gtaatgaccg tatgaaaacc aactatcacg ataagctggc ggcgatcgaa 4320 aaagatcgtg attctgcgcg taaagattgg aagaaaatta acaatatcaa agaaatgaaa 4380 gaaggctatc tgagccaagt ggtgcacgag atcgcaaaac tggtgattga atataacgct 4440 atcgtggttt tcgaagatct gaactttggt tttaaacgtg gtcgcttcaa agtagaaaaa 4500 caggtgtacc aaaaactgga aaaaatgctg attgaaaaac tgaactatct ggtttttaaa 4560 gacaacgaat ttgacaaaac gggtggcgta ctccgtgcct atcagctgac cgctccgttc 4620 gaaacgttca agaaaatggg taaacaaacg gggattatct attatgtgcc agctggtttc 4680 acctccaaga tttgtccagt tacgggcttc gttaaccagc tgtacccgaa atacgagagc 4740 gttagcaaat ctcaagaatt tttcagcaaa ttcgacaaga tctgctataa tctggataaa 4800 ggctatttcg agttcagctt cgattacaaa aacttcggcg ataaagcggc taaaggtaag 4860 tggactattg ctagctttgg tagccgtctg attaactttc gcaactccga caaaaaccat 4920 aattgggaca cgcgtgaagt gtatccgacc aaagaactgg aaaaattact gaaagactat 4980 tccatcgaat atggtcatgg ggagtgcatt aaagcggcga tttgcggtga atccgataag 5040 aaatttttcg ccaaactgac cagcgtgctt aacaccattc tgcaaatgcg taattctaaa 5100 acgggtacgg agctggacta cctgatttct ccggtagccg acgttaacgg caacttcttc 5160 gattctcgtc aagcaccgaa aaatatgcca caagacgcgg atgccaacgg tgcataccat 5220 atcggcttaa aaggcttaat gttattaggc cgtatcaaga ataatcagga gggcaagaaa 5280 ttaaatctgg ttatcaaaaa cgaagaatac ttcgagttcg ttcagaatcg taacaattaa 5340 tgtatgctta agcagctcgg taccaaagac gaacaataag acgctgaaaa gcgtcttttt 5400 tcgttttggt cctgttgcgg cgcgatagtg tgaacatgct atagacttct ggtgctaccc 5460 gactgacagc tagctcagtc ctaggtataa tgctagcaat ttctactgtt gtagatagaa 5520 gagcttcatc gctcttcatc gagacgaaca ataaggcctc cctaacgggg ggcctttttt 5580 attgataaca aaagtaactt cgagcttgtc tacctcctag cactttatag ctagctcagc 5640 ccttggtaca atgctagcgt tttcattaaa gaggagaaag gaagccatga gtaaaggtga 5700 ggaattattt actggtgttg ttccgatctt agttgaactg gacggcgatg ttaacggtca 5760 taaattcagt gttcgtggtg aaggtgaagg tgatgcaacc aacggtaagc tgaccctgaa 5820 attcatctgc actactggaa aattaccagt accgtggcct actctggtga ctaccctgac 5880 ctatggtgtt cagtgttttt ctcgttaccc tgaccacatg aagcaacatg atttcttcaa 5940 atctgcaatg ccggaaggtt atgtacagga gcgcaccatt tctttcaaag acgatggcac 6000 gtataaaacc cgtgcagagg ttaaatttga aggtgacact ctggtgaatc gtattgaact 6060 gaaaggcatt gatttcaaag aggacggcaa tattttaggc cacaaactgg aatataactt 6120 caactcccat aacgtttaca tcaccgcaga caaacagaag aacggtatca aagctaactt 6180 caaaattcgc cataacgttg aagatggtag cgtacagctg gcggatcatt accaacagaa 6240 cactccgatt ggagatgctc ctgttttact gccggataac cactacctgt ccacccagtc 6300 taaactgtcg aaggatccga acgaaaagcg cgaccacatg gtgttattag agttcgttac 6360 cgctagtggt atcacgcacg gtatggatga actctacaaa taagacgaac aataagggga 6420 gcgggaaacc gctccccttt tttattgata acaaaagtaa attgcacgct gatagtctcc 6480 caattgcgaa ggaccaaaac gaaaaaacac cctttcgggt gtcttttctg gaatttggta 6540 ccgagtacta ggtatcgtgt aagtagcgaa ggcccgtacg cgagataaac tgctaggcaa 6600 ccgcgactct acgactggtg ctcgatttaa tttcgctgac gtaaagaaat tatcggcagt 6660 gcgtcaactg ccgtatcttt atcttaatta ggtagttgga caagcccttg aaagaaatag 6720 caagagcctg cctctctatt gaagtcacgg cgaaagtcgg gtagaaatca aagaaagcag 6780 aaattaaatc ggagtaacac taaggtggga taactccgta actgactacg cctttctcta 6840 gactttactt gaccagatac actgtctttg acacgttgaa ggattagagc aatcaaatcc 6900 aagactggct aagcacgaag caactcttga gtgttaaaaa gttatctcct gtattcggga 6960 agcgggtact agaagattgc agggactccg acgttaagta aattacaaag taataagtat 7020 cgttcaggat cacgttaccg caataagaag cgagaataat ataatttccg aagtgcttac 7080 cccagtagtg actattccta taacccttct gagtgtccgg aggcggaaat ttgccacgaa 7140 agagaaagta tttccccgac aataataaag gggcgctcct cagcttttcc acttggttgg 7200 gtaagctagg caactctgaa aggagtttcg gcgaattgaa gccgacagct ttgaattgtt 7260 ttaggggcgt tattcgaggg caatcggagc taacttcaag actacttctt tgttgaatac 7320 taaatagtgc aaaggtcgtg tttcctcaag gatactccgc taacaatata ggattccaat 7380 cagatcagc actggcggta cgggtgttgc ggtgaggcgt tcgggtttac ggctcgaagc 7440 tagcacggta ggaagcctga caatcaccaa gcaaaagggc cgtcgaaggc ccacaagata 7500 cgaaagctct cgaagcctta tccttgaccg atccacctat ttaggcagtt acgcacaaaa 7560 gctacccaat aatccgtgac aggcacaata tcacggaaca aaaccgaaaa ctctcgtaca 7620 cggttaggtt ttcgctagga agaataaacc tctatcttga ttataagaag gctccccaag 7680 cacccccaaa accgaaatag cggtttgcaa taagggacaa gttacgagtg tagacacgca 7740 gaattatcca gcctttagtc tttaggaagg caaagctatt gtacgcggta gccgtcgtag 7800 caatttacca actgtagaat tattggacac acgtaggaag ggcttacagt tgaagtttaa 7860 taaggtcaca cgcaaaaccg ctaaggaata atcgcaccgt tagcgaaaga atatttcaga 7920 gcggttagta aaggttgagt aaagtgagat tccaaagtga gcctttataa aaagtaaaga 7980 gctataataa aaccgtcgag cagaaaacaa tcgcctgaaa tctcaagcac gttgcccttt 8040 ctaacgtcgc taaggtttcg taaacccgtt tgattaggaa gaagaataag taacccgatt 8100 aggtttgaga tcgcgggtta tcggtttgga ttaaaagtgg ataccagcgg agtcaacgcc 8160 gacgcaaacg tacagtgatc caatcctgtt gcacggtcaa gcacaatcag ctcgcaagat 8220 cttggaatag tgtgcccaac agtttagttg agggccacgt tccgactaca agttgcttca 8280 agaggggaat ttggatttgg caatagcccc ccgtttctac ctcaagaggc gacgagtatt 8340 aaccgcgcca gctgtcggca caagggccaa agaagattcc aatttcttat tcccgaataa 8400 cctccgaatc cctgcgggaa aatcaccgac cgaatagcct agaagcaagg gggaacagat 8460 aggtataatt agcttaagag agtaccagcc gtgacaacag cgtagtaacc acaaacttac 8520 gctggggctt ctttggcgga tttttacaga tactaacaag gtgatttgaa gtaccttagt 8580 tgaggattta aacgcgctat ccggtaatct ccaaattggg aaataccgtt caaagagggc 8640 tagaattact taaaagcctt cacaccgcct gcgctatacg cgcccactct cccgtttatc 8700 cgtccaagcg gaagcagggc gatcctccgc taagatattc ttacgtgtaa cgtagctaag 8760 tatcccaaat agctggcgta cgcgttgaac accgcctaga ggatcgtgac tcgccggacg 8820 agcgtgttat tggggactta cgccagcgta gactacaacg cgcccagatt aaccctgcac 8880 gtattgcctt gaataacgta ctaatctctc cggctctcga caatctatcg agcgactcga 8940 ttatcaacgg gtgtcttgca gttctaatct cttgcccccg cccgtaatag cctccaagag 9000 attgaagata gtaaagggca agagctgatt cggcgttgaa ggatagcgga ctttcggtca 9060 accacaattc cccactcgac aaaaccagcc gtgcgaataa ctctgaaagt acaagcaacc 9120 caagagggct gagcctaaac tcagctaatt cctaagtgag ctaaagactc gaagtgacag 9180 ctcttaataa atagagcggg aacgtcgaac ggtcgtgaaa gtaatagtac aacgggtatt 9240 aacttactga ggatattgct tgaagctgta ccgttttatt gggtgaacga ataagatcca 9300 gcaattcagc caaagaagct accaattttt agtttaagag tgtcacgtct gacctcgcgg 9360 gtagattgcc gaacgtagag cttacgagcc agcggaaaca gtagccgcag gataagtaag 9420 gggagtaagt gatcgaacga atcagaagtg acaatatact taggctggat ctcgtcccgt 9480 gaatcccaac cctcaccaac tacgagataa gaggtaagcc aaaaatcgac ttggtggcga 9540 ccaacgactg ttccccccct gtaactaatc gttccgtcaa aacctgactt acttcaaggc 9600 caattccaag cgcaaacaat accgtcctag ttcttcggtt aagtttccga agtaggagtg 9660 agcctacctc cgtttgcgtc ttgttaccac tgacccagct atttactttg tattgcctgc 9720 aatcgaattt ctgaactctc agatagtggg gataacggga aagttcctat atttgcgaac 9780 taacttagcc gtccacctcg aagctaccta ctcacaccca ccccgcgcgg ggtaaataag 9840 gcactaatcc cagctgagag ctggcgtagc acttagccac aagttaatta acagttgtct 9900 ggtagtttgg cggtattagg aagatcctag aagcaaggca gagttagttc taacctaaag 9960 ccacaaataa gacaggttgc caaagcccgc cggaaattaa atcttgctca gttcggtaac 10020 ggagtttccc tcccgcgtac ttaattccca ataagaaacg cgcccaagtc ctatcaggca 10080 aaattcagcc ccttcccgtg ttagaacgag ggtaaaaata caagccgatt gaacaagggt 10140 tgggggcttc aaatcgtcgt ttaccccact ttacaacgga gattaagtag ttcaccctat 10200 agtacgaagc agaactattt cgaggggcgt gcaataatcg aatcttctgc ggttgactta 10260 acacgctagg gacgtgccct cgattcaatc gaaggtactc ctactcagac tgcctcacac 10320 ccagctagtc actgagcgat aaaattgacc cgccctctag ggaagcgagt acgtcccaaa 10380 gggctccgga cagggctata taggagagtt tgatctcgcc ccgacaactg caaccctcaa 10440 ctcccttaga taatattgtt agccgaagtt gcacgacccg ccgtccacgg actgctctta 10500 gggtgtggct ccttaatctg acaacgtgca acccctatcg aagtcgattg tttctgcgaa 10560 aggtgttgtc ctaatagtcc cgaaatttgg cccttgtagg tgtgaaacca cttagcttcg 10620 cgccgtagtc ctaaaggccc acctattgac tttgtttcgg gtagcactag gaatcttaac 10680 aatttgaatt tggacgtgga acgcgtacac cttaatctcc gaataattct aggatttgg 10740 aagtcctcta cgttgacaca cctacactgc tcgaagtaaa tatacgaata acgcgggcct 10800 cgcggagccg ttccgaatcg tcacgtgttc gtttactgtt aattggtggc aaataagcaa 10860 tatcgtagtc cgtcaggccc agccctgtta tccacggcgt tatttgtcaa attgcgtaga 10920 actggattga ctgcctgaca atacctaatt atcggtacga agtccccgaa tctgtcgggc 10980 tatttcacta atactttcca aacgccccgt atccaagaag aacgaattta tccacgctcc 11040 cgtctttggg acgaataccg ctacaagtgg acagaggatc ggtacgggcc tctaataaat 11100 ccaacactct acgccctctt caagagctag aagaacaggg tgcagttgga aagggaatta 11160 tttcgtaagg cgagccaata ccgtaattaa ttcggaagag ttaacacgat tggaagtagg 11220 aatagtttct aaccacggtt actaatccta ataacggaac gctgtctgat agattagtgt 11280 cagcgctcgg taccaaagaa aaataaaaag acgctgaaaa gcgtcttttt atttttcggt 11340 ccagtgtaac tcaggcaaaa gcacgtaata ttcgtacttt cttcctccgt aagcgtcacc 11400 cacattcctt aaagagtgca tgtgcatatt ttgttatcaa taaaaaaggc cgcgatttgc 11460 ggccttattg ttcgtcttgc cggattacgc cccgccctgc cactcatcgc agtattgttg 11520 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacttggat 11580 cgccagtggc attaacacct tgtcgccttg cgtataatat tttcccatag tgaaaacggg 11640 ggcgaagaag ttgtccatat ttgctacgtt taaatcaaaa ctggtgaaac tcacccacgg 11700 attggcactg acgaaaaaca tattttcgat aaacccttta gggaaatatg ctaagttttc 11760 accgtaacac gccacatctt gactatatat gtgtagaaac tgccggaaat cgtcgtggta 11820 ttctgaccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg 11880 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaact ccggatgtgc 11940 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt 12000 tacggttttt aaaaaggccg taatatccag ctgaacggtt tggttatagg tgcactgagc 12060 aactgactgg aatgcctcaa aatgttcttt acgatgccat tgacttatat caactgtagt 12120 atatccagtg atttttttct ccattttagc ttccttagct tgcgaaatct cgataactca 12180 aaaaatagta gtgatcttat ttcattatgg tgaaagttgt cttacgtgca acattttcgc 12240 aaaaagttgg cgctttatca ac 12262 <210> 9 <211> 7300 <212> DNA <213> Artificial Sequence <220> <223> p7.3kb (p513) <400> 9 cctttaggga aatatgctaa gttttcaccg taacacgcca catcttgact atatatgtgt 60 agaaactgcc ggaaatcgtc gtggtattct gaccagagcg atgaaaacgt ttcagtttgc 120 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc 180 attgccatac gaaactccgg atgtgcattc atcaggcggg caagaatgtg aataaaggcc 240 ggataaaact tgtgcttatt tttctttacg gtttttaaaa aggccgtaat atccagctga 300 acggtttggt tataggtgca ctgagcaact gactggaatg cctcaaaatg ttctttacga 360 tgccattgac ttatatcaac tgtagtatat ccagtgattt ttttctccat tttagcttcc 420 ttagcttgcg aaatctcgat aactcaaaaa atagtagtga tcttatttca ttatggtgaa 480 agttgtctta cgtgcaacat tttcgcaaaa agttggcgct ttatcaacac tgtccctcct 540 gttcagctac tgacggtact gcggaactga ctaaagtagt gcgtaacggc aaaagcaccg 600 ccggacatct gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt 660 aagtgaagtg cttcatgtgg caggagaaaa aaggctgcat cggtgcgtca gcagaatatg 720 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg 780 tggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata 840 cttaacaggg aagtgagagg gtcgcggcaa agccgttttt ccataggctc cgcccccctg 900 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacctgaca ggactataaa 960 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt 1020 ttgccggtgt cattcctctg ttacggccga gtttgtctca ttccacgcct gacactcagt 1080 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac 1140 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca 1200 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg 1260 ataaggctaa actgaaagga caagttttgg cgactgcgct cctccaagcc agttacctcg 1320 gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc 1380 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa 1440 tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag cactttatag 1500 ctagctcagc ccttggtaca atgctagcgt tttcattaaa gaggagaaag gaagccatga 1560 gtaaaggtga ggaattattt actggtgttg ttccgatctt agttgaactg gacggcgatg 1620 ttaacggtca taaattcagt gttcgtggtg aaggtgaagg tgatgcaacc aacggtaagc 1680 tgaccctgaa attcatctgc actactggaa aattaccagt accgtggcct actctggtga 1740 ctaccctgac ctatggtgtt cagtgttttt ctcgttaccc tgaccacatg aagcaacatg 1800 atttcttcaa atctgcaatg ccggaaggtt atgtacagga gcgcaccatt tctttcaaag 1860 acgatggcac gtataaaacc cgtgcagagg ttaaatttga aggtgacact ctggtgaatc 1920 gtattgaact gaaaggcatt gatttcaaag aggacggcaa tattttaggc cacaaactgg 1980 aatataactt caactcccat aacgtttaca tcaccgcaga caaacaaaag aacggtatca 2040 aagctaactt caaaattcgc cataacgttg aagacggtag cgtacagctg gcggatcatt 2100 accaacagaa cactccgatt ggagatgctc ctgttttact gccggataac cactacctgt 2160 ccacccagtc taaactgtcg aaggatccga acgaaaagcg cgaccacatg gtgttattag 2220 agttcgttac cgctagtggt atcacgcacg gtatggatga actctacaaa taagtcagtt 2280 tcacctgttt tacgttaaaa cccgcttcgg cgggttttta cttttgggtt tagccgaacg 2340 ccccaaaaag cctcgctttc agcacctgtc gtttcctttc ttttcagagg gtattttaaa 2400 taaaaacatt aagttatgac gaagaagaac ggaaacgcct taaaccggaa aattttcata 2460 aatagcgaaa acccgcgagg tcgccgcccc gtaacctgtc ggatcaccgg aaagaacctg 2520 taaagtgata atgattatca tctacatatc acaacgtgcg taaagggact ataacaagac 2580 gcaaacggag gtaggctcac tcctacttcg gaaacttaac cgaagaacta ggacggtatt 2640 gtttgcgctt ggaattggcc ttgaagtaag tcaggttttg acggaacgat tagttacagg 2700 gggggaacag tcgttggtcg ccaccaagtc gatttttggc ttacctctta tctcgtagtt 2760 ggtgagggtt gggattcacg ggacgagatc cagcctaagt atattgtcac ttctgattcg 2820 ttcgatcact tactcccctt acttatcctg cggctactgt ttccgctggc tcgtaagctc 2880 tacgttcggc aatctacccg cgaggtcaga cgtgacactc ttaaactaaa aattggtagc 2940 ttctttggct gaattgctgg atcttattcg ttcacccaat aaaacggtac agcttcaagc 3000 aatatcctca gtaagttaat acccgttgta ctattacttt cacgaccgtt cgacgttccc 3060 gctctattta ttaagagctg tcacttcgag tctttagctc acttaggaat tagctgagtt 3120 taggctcagc cctcttgggt tgcttgtact ttcagagtta ttcgcacggc tggttttgtc 3180 gagtggggaa ttgtggttga ccgaaagtcc gctatccttc aacgccgaat cagctcttgc 3240 cctttactat cttcaatctc ttggaggcta ttacgggcgg gggcaagaga ttagaactgc 3300 aagacacccg ttgataatcg agtcgctcga tagattgtcg agagccggag agattagtac 3360 gttattcaag gcaatacgtg cagggttaat ctgggcgcgt tgtagtctac gctggcgtaa 3420 gtccccaata acacgctcgt ccggcgagtc acgatcctct aggcggtgtt caacgcgtac 3480 gccagctatt tgggatactt agctacgtta cacgtaagaa tatcttagcg gaggatcgcc 3540 ctgcttccgc ttggacggat aaacgggaga gtgggcgcgt atagcgcagg cggtgtgaag 3600 gcttttaagt aattctagcc ctctttgaac ggtatttccc aatttggaga ttaccggata 3660 gcgcgtttaa atgagtgtca gagaaacgga agccgaagtc tttccattcc ggatgtttgg 3720 aaatgctctg tttatagaag tcgatgaact tacggcagtc ctctatatta aattcgaatt 3780 tttcataccc tttctgcggg ctaccgtttt tagtgtgcgt gctatgattg cggatgcgca 3840 gaatatcctc agacgggttg tagaatttga tgcttttcgc ggaaaagaac actttcggta 3900 acattttatt cgcacccggc aggagcttgt acacgatttt cttatagcct tcacccttgt 3960 tttctttgat cgctttatcg tcgaagatct tgttgttctt cttgttcatt acgcccagat 4020 agtatttgtc gtctttgatg aacaggattg cggtgttgtc cggctctttg ttcttatccc 4080 agccgttcgc cagcgtgctg ttttcgaagt tcagtttgaa tttctcgtca gagtaaggct 4140 tctgcgtgat gtagttgcgg attttattgt agagagggac gatgtttgcc agttcgaagt 4200 aacattcttc gaacaccaga tagaagtgtt catctttatc cagaatgttc gccttgtcct 4260 cgctctggct gatgtggaag attttgagct tgtgtaataa gttattcgtc tgatctaata 4320 agtctttaat tgctttcaca tcgtcctccg cagatgcttg aagcagatct ttcttaccct 4380 gattctggta cttgatagag atctgcgcca gattgtcttt gttttgagca atttcgtcga 4440 agatcatcgg gattgccgca aagttcgcca gaatttcctc aaaacgacac tgtttatcaa 4500 tatcacgatg tttattaaat tcctcaagtg ccagtttgat agtttctaag ctcaggtatt 4560 tagctttttc tgttttcttt gcaatcagtt cctgttcctt cttggacggg ttgtccagat 4620 ttttcggcgc gatttgttgg gtgatgtatt ccaaaactgc cgtgccgatc acgctatagt 4680 catcgaaaac ttgttgactg agatcggtca gagatttgtc gtttttaaag taaatcttag 4740 acagatctag tttctgcgct ttgaggtcgt caaagagcag ggacagagtt tctttaatag 4800 atttctcttc cacggttttg aacgccgcaa tctgctcata aaagctctgc atcgtggtga 4860 caacgtcgct atcatcttcc agtttatcaa ttacgaagga tttagattcg gtgtccgata 4920 aaatctgttt aaacagaacg gacattttat actttttcag ggttttgtcg ttgatttgtt 4980 ggctatacag gttaatgtat tcgttgatgc ccttacgctt ggtgttttcg ccgttaacaa 5040 atttgccacc aataatggtg ttgaatttgg tgatgccaga ttgattcagg taattgttga 5100 aattagcgat ttcgaaaacc tcgtccagtg agaaaacacg ctggttaact tcggaggttt 5160 tatagtcgat gtcgaaggtc agttcttccg ccagatcttt cttgatctgt tcatagttaa 5220 tagcttccgg tgctttgtct ttcagagatt catatttcgc tttgttttcc agaaacttcg 5280 gcaggttgtc gtccacgata cgataaataa tagaggtcgg aatatcgttg ctcgaatata 5340 cattcttacg gttttcatga aaacctttga aatacgtcgt ccagcctttg aaagacttga 5400 tgatttcggt attcgaatat gacctgatca aagataaacg tttcaccgaa gataagttct 5460 ttttccactg tccgattacc atcaacttca aatctagcgg tgcgaacaag ttcaacgatg 5520 aaattaactt attactgaaa gagaaagcta atgacgtaca catcttatct attgatcgcg 5580 gtgaacgtca tttagcatac tatacactgg tagatggtaa aggtaatatt attaaacagg 5640 atactttcaa tattatcggt aatgaccgta tgaaaaccaa ctatcacgat aagctggcgg 5700 cgatcgaaaa agatcgtgat tctgcgcgta aagattggaa gaaaattaac aatatcaaag 5760 aaatgaaaga aggctatctg agccaagtgg tgcacgagat cgcaaaactg gtgattgaat 5820 ataacgctat cgtggttttc gaagatctga actttggttt taaacgtggt cgcttcaaag 5880 tagaaaaaca ggtgtaccaa aaactggaaa aaatgctgat tgaaaaactg aactatctgg 5940 tttttaaaga caacgaattt gacaaaacgg gtggcgtact ccgtgcctat cagctgaccg 6000 ctccgttcga aacgttcaag aaaatgggta aacaaacggg gattatctat tatgtgccag 6060 ctggtttcac ctccaagatt tgtccagtta cgggcttcgt taaccagctg tacccgaaat 6120 acgagagcgt tagcaaatct caagaatttt tcagcaaatt cgacaagatc tgctataatc 6180 tggataaagg ctatttcgag ttcagcttcg attacaaaaa cttcggcgat aaagcggcta 6240 aaggtaagtg gactattgct agctttggta gccgtctgat taactttcgc aactccgaca 6300 aaaaccataa ttgggacacg cgtgaagtgt atccgaccaa agaactggaa aaattactga 6360 aagactattc cggacactca gaagggttat aggaatagtc actactgggg taagcacttc 6420 ggaaattata ttattctcgc ttcttattgc ggtaacgtga tcctgaacga tacttattac 6480 tttgtaattt acttaacgtc ggagtccctg caatcttcta gtacccgctt cccgaataca 6540 ggagataact ttttaacact caagagttgc ttcgtgctta gccagtcttg gatttgattg 6600 ctctaatcct tcaacgtgtc aaagacagtg tatctggtca agtaaagtct agagaaaggc 6660 gtagtcagtt acggagttat cccaccttag tgttactccg atttaatttc tgctttcttt 6720 gatttctacc cgactttcgc cgtgacttca atagagaggc aggctcttgc tatttctttc 6780 aagggcttgt ccaactacct aattaagata aagatacggc agttgacgca ctgccgataa 6840 tttctttacg tcagcgaaat taaatcgagc accagtcgta gagtcgcggt tgcctagcag 6900 tttatctcgc gtacgggcct tcgctactta cacgatacct agtacgtgga ttcgggtagc 6960 accagaagtc tatagcatgt gcataccttt ggtcgaaaaa aaaagcccgc actgtcaggt 7020 gcgggctttt ttcagtgttt ccttgccgga ttacgccccg ccctgccact catcgcagta 7080 ttgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac 7140 ttggatcgcc agtggcatta acaccttgtc gccttgcgta taatattttc ccatagtgaa 7200 aacgggggcg aagaagttgt ccatatttgc tacgtttaaa tcaaaactgg tgaaactcac 7260 ccagggattg gcactgacga aaaacatatt ttcgataaac 7300 <210> 10 <211> 1132 <212> PRT <213> Escherichia phage lambda (Bacteriophage lambda) <220> <221> MISC_FEATURE <222> (814)..(821) <223> box 1 in Figure 7 <220> <221> MISC_FEATURE <222> (958)..(966) <223> box 2 in Figure 7 <400> 10 Met Gly Lys Gly Ser Ser Lys Gly His Thr Pro Arg Glu Ala Lys Asp 1 5 10 15 Asn Leu Lys Ser Thr Gln Leu Leu Ser Val Ile Asp Ala Ile Ser Glu 20 25 30 Gly Pro Ile Glu Gly Pro Val Asp Gly Leu Lys Ser Val Leu Leu Asn 35 40 45 Ser Thr Pro Val Leu Asp Thr Glu Gly Asn Thr Asn Ile Ser Gly Val 50 55 60 Thr Val Val Phe Arg Ala Gly Glu Gln Glu Gln Thr Pro Pro Glu Gly 65 70 75 80 Phe Glu Ser Ser Gly Ser Glu Thr Val Leu Gly Thr Glu Val Lys Tyr 85 90 95 Asp Thr Pro Ile Thr Arg Thr Ile Thr Ser Ala Asn Ile Asp Arg Leu 100 105 110 Arg Phe Thr Phe Gly Val Gln Ala Leu Val Glu Thr Thr Ser Lys Gly 115 120 125 Asp Arg Asn Pro Ser Glu Val Arg Leu Leu Val Gln Ile Gln Arg Asn 130 135 140 Gly Gly Trp Val Thr Glu Lys Asp Ile Thr Ile Lys Gly Lys Thr Thr 145 150 155 160 Ser Gln Tyr Leu Ala Ser Val Val Met Gly Asn Leu Pro Pro Arg Pro 165 170 175 Phe Asn Ile Arg Met Arg Arg Met Thr Pro Asp Ser Thr Thr Asp Gln 180 185 190 Leu Gln Asn Lys Thr Leu Trp Ser Ser Tyr Thr Glu Ile Ile Asp Val 195 200 205 Lys Gln Cys Tyr Pro Asn Thr Ala Leu Val Gly Val Gln Val Asp Ser 210 215 220 Glu Gln Phe Gly Ser Gln Gln Val Ser Arg Asn Tyr His Leu Arg Gly 225 230 235 240 Arg Ile Leu Gln Val Pro Ser Asn Tyr Asn Pro Gln Thr Arg Gln Tyr 245 250 255 Ser Gly Ile Trp Asp Gly Thr Phe Lys Pro Ala Tyr Ser Asn Asn Met 260 265 270 Ala Trp Cys Leu Trp Asp Met Leu Thr His Pro Arg Tyr Gly Met Gly 275 280 285 Lys Arg Leu Gly Ala Ala Asp Val Asp Lys Trp Ala Leu Tyr Val Ile 290 295 300 Gly Gln Tyr Cys Asp Gln Ser Val Pro Asp Gly Phe Gly Gly Thr Glu 305 310 315 320 Pro Arg Ile Thr Cys Asn Ala Tyr Leu Thr Thr Gln Arg Lys Ala Trp 325 330 335 Asp Val Leu Ser Asp Phe Cys Ser Ala Met Arg Cys Met Pro Val Trp 340 345 350 Asn Gly Gln Thr Leu Thr Phe Val Gln Asp Arg Pro Ser Asp Lys Thr 355 360 365 Trp Thr Tyr Asn Arg Ser Asn Val Val Met Pro Asp Asp Gly Ala Pro 370 375 380 Phe Arg Tyr Ser Phe Ser Ala Leu Lys Asp Arg His Asn Ala Val Glu 385 390 395 400 Val Asn Trp Ile Asp Pro Asn Asn Gly Trp Glu Thr Ala Thr Glu Leu 405 410 415 Val Glu Asp Thr Gln Ala Ile Ala Arg Tyr Gly Arg Asn Val Thr Lys 420 425 430 Met Asp Ala Phe Gly Cys Thr Ser Arg Gly Gln Ala His Arg Ala Gly 435 440 445 Leu Trp Leu Ile Lys Thr Glu Leu Leu Glu Thr Gln Thr Val Asp Phe 450 455 460 Ser Val Gly Ala Glu Gly Leu Arg His Val Pro Gly Asp Val Ile Glu 465 470 475 480 Ile Cys Asp Asp Asp Tyr Ala Gly Ile Ser Thr Gly Gly Arg Val Leu 485 490 495 Ala Val Asn Ser Gln Thr Arg Thr Leu Thr Leu Asp Arg Glu Ile Thr 500 505 510 Leu Pro Ser Ser Gly Thr Ala Leu Ile Ser Leu Val Asp Gly Ser Gly 515 520 525 Asn Pro Val Ser Val Glu Val Gln Ser Val Thr Asp Gly Val Lys Val 530 535 540 Lys Val Ser Arg Val Pro Asp Gly Val Ala Glu Tyr Ser Val Trp Glu 545 550 555 560 Leu Lys Leu Pro Thr Leu Arg Gln Arg Leu Phe Arg Cys Val Ser Ile 565 570 575 Arg Glu Asn Asp Asp Gly Thr Tyr Ala Ile Thr Ala Val Gln His Val 580 585 590 Pro Glu Lys Glu Ala Ile Val Asp Asn Gly Ala His Phe Asp Gly Glu 595 600 605 Gln Ser Gly Thr Val Asn Gly Val Thr Pro Pro Ala Val Gln His Leu 610 615 620 Thr Ala Glu Val Thr Ala Asp Ser Gly Glu Tyr Gln Val Leu Ala Arg 625 630 635 640 Trp Asp Thr Pro Lys Val Val Lys Gly Val Ser Phe Leu Leu Arg Leu 645 650 655 Thr Val Thr Ala Asp Asp Gly Ser Glu Arg Leu Val Ser Thr Ala Arg 660 665 670 Thr Thr Glu Thr Thr Tyr Arg Phe Thr Gln Leu Ala Leu Gly Asn Tyr 675 680 685 Arg Leu Thr Val Arg Ala Val Asn Ala Trp Gly Gln Gln Gly Asp Pro 690 695 700 Ala Ser Val Ser Phe Arg Ile Ala Ala Pro Ala Ala Pro Ser Arg Ile 705 710 715 720 Glu Leu Thr Pro Gly Tyr Phe Gln Ile Thr Ala Thr Pro His Leu Ala 725 730 735 Val Tyr Asp Pro Thr Val Gln Phe Glu Phe Trp Phe Ser Glu Lys Gln 740 745 750 Ile Ala Asp Ile Arg Gln Val Glu Thr Ser Thr Arg Tyr Leu Gly Thr 755 760 765 Ala Leu Tyr Trp Ile Ala Ala Ser Ile Asn Ile Lys Pro Gly His Asp 770 775 780 Tyr Tyr Phe Tyr Ile Arg Ser Val Asn Thr Val Gly Lys Ser Ala Phe 785 790 795 800 Val Glu Ala Val Gly Arg Ala Ser Asp Asp Ala Glu Gly Tyr Leu Asp 805 810 815 Phe Phe Lys Gly Lys Ile Thr Glu Ser His Leu Gly Lys Glu Leu Leu 820 825 830 Glu Lys Val Glu Leu Thr Glu Asp Asn Ala Ser Arg Leu Glu Glu Phe 835 840 845 Ser Lys Glu Trp Lys Asp Ala Ser Asp Lys Trp Asn Ala Met Trp Ala 850 855 860 Val Lys Ile Glu Gln Thr Lys Asp Gly Lys His Tyr Val Ala Gly Ile 865 870 875 880 Gly Leu Ser Met Glu Asp Thr Glu Glu Gly Lys Leu Ser Gln Phe Leu 885 890 895 Val Ala Ala Asn Arg Ile Ala Phe Ile Asp Pro Ala Asn Gly Asn Glu 900 905 910 Thr Pro Met Phe Val Ala Gln Gly Asn Gln Ile Phe Met Asn Asp Val 915 920 925 Phe Leu Lys Arg Leu Thr Ala Pro Thr Ile Thr Ser Gly Gly Asn Pro 930 935 940 Pro Ala Phe Ser Leu Thr Pro Asp Gly Lys Leu Thr Ala Lys Asn Ala 945 950 955 960 Asp Ile Ser Gly Ser Val Asn Ala Asn Ser Gly Thr Leu Ser Asn Val 965 970 975 Thr Ile Ala Glu Asn Cys Thr Ile Asn Gly Thr Leu Arg Ala Glu Lys 980 985 990 Ile Val Gly Asp Ile Val Lys Ala Ala Ser Ala Ala Phe Pro Arg Gln 995 1000 1005 Arg Glu Ser Ser Val Asp Trp Pro Ser Gly Thr Arg Thr Val Thr Val 1010 1015 1020 Thr Asp Asp His Pro Phe Asp Arg Gln Ile Val Val Leu Pro Leu Thr 1025 1030 1035 1040 Phe Arg Gly Ser Lys Arg Thr Val Ser Gly Arg Thr Thr Tyr Ser Met 1045 1050 1055 Cys Tyr Leu Lys Val Leu Met Asn Gly Ala Val Ile Tyr Asp Gly Ala 1060 1065 1070 Ala Asn Glu Ala Val Gln Val Phe Ser Arg Ile Val Asp Met Pro Ala 1075 1080 1085 Gly Arg Gly Asn Val Ile Leu Thr Phe Thr Leu Thr Ser Thr Arg His 1090 1095 1100 Ser Ala Asp Ile Pro Tyr Thr Phe Ala Ser Asp Val Gln Val Met 1105 1110 1115 1120 Val Ile Lys Lys Gln Ala Leu Gly Ile Ser Val Val 1125 1130 <210> 11 <211> 1132 <212> PRT <213> Artificial Sequence <220> <223> gpJ 591 <400> 11 Met Gly Lys Gly Ser Ser Lys Gly His Thr Pro Arg Glu Ala Lys Asp 1 5 10 15 Asn Leu Lys Ser Thr Gln Leu Leu Ser Val Ile Asp Ala Ile Ser Glu 20 25 30 Gly Pro Ile Glu Gly Pro Val Asp Gly Leu Lys Ser Val Leu Leu Asn 35 40 45 Ser Thr Pro Val Leu Asp Thr Glu Gly Asn Thr Asn Ile Ser Gly Val 50 55 60 Thr Val Val Phe Arg Ala Gly Glu Gln Glu Gln Thr Pro Pro Glu Gly 65 70 75 80 Phe Glu Ser Ser Gly Ser Glu Thr Val Leu Gly Thr Glu Val Lys Tyr 85 90 95 Asp Thr Pro Ile Thr Arg Thr Ile Thr Ser Ala Asn Ile Asp Arg Leu 100 105 110 Arg Phe Thr Phe Gly Val Gln Ala Leu Val Glu Thr Thr Ser Lys Gly 115 120 125 Asp Arg Asn Pro Ser Glu Val Arg Leu Leu Val Gln Ile Gln Arg Asn 130 135 140 Gly Gly Trp Val Thr Glu Lys Asp Ile Thr Ile Lys Gly Lys Thr Thr 145 150 155 160 Ser Gln Tyr Leu Ala Ser Val Val Met Gly Asn Leu Pro Pro Arg Pro 165 170 175 Phe Asn Ile Arg Met Arg Arg Met Thr Pro Asp Ser Thr Thr Asp Gln 180 185 190 Leu Gln Asn Lys Thr Leu Trp Ser Ser Tyr Thr Glu Ile Ile Asp Val 195 200 205 Lys Gln Cys Tyr Pro Asn Thr Ala Leu Val Gly Val Gln Val Asp Ser 210 215 220 Glu Gln Phe Gly Ser Gln Gln Val Ser Arg Asn Tyr His Leu Arg Gly 225 230 235 240 Arg Ile Leu Gln Val Pro Ser Asn Tyr Asn Pro Gln Thr Arg Gln Tyr 245 250 255 Ser Gly Ile Trp Asp Gly Thr Phe Lys Pro Ala Tyr Ser Asn Asn Met 260 265 270 Ala Trp Cys Leu Trp Asp Met Leu Thr His Pro Arg Tyr Gly Met Gly 275 280 285 Lys Arg Leu Gly Ala Ala Asp Val Asp Lys Trp Ala Leu Tyr Val Ile 290 295 300 Gly Gln Tyr Cys Asp Gln Ser Val Pro Asp Gly Phe Gly Gly Thr Glu 305 310 315 320 Pro Arg Ile Thr Cys Asn Ala Tyr Leu Thr Thr Gln Arg Lys Ala Trp 325 330 335 Asp Val Leu Ser Asp Phe Cys Ser Ala Met Arg Cys Met Pro Val Trp 340 345 350 Asn Gly Gln Thr Leu Thr Phe Val Gln Asp Arg Pro Ser Asp Lys Thr 355 360 365 Trp Thr Tyr Asn Arg Ser Asn Val Val Met Pro Asp Asp Gly Ala Pro 370 375 380 Phe Arg Tyr Ser Phe Ser Ala Leu Lys Asp Arg His Asn Ala Val Glu 385 390 395 400 Val Asn Trp Ile Asp Pro Asn Asn Gly Trp Glu Thr Ala Thr Glu Leu 405 410 415 Val Glu Asp Thr Gln Ala Ile Ala Arg Tyr Gly Arg Asn Val Thr Lys 420 425 430 Met Asp Ala Phe Gly Cys Thr Ser Arg Gly Gln Ala His Arg Ala Gly 435 440 445 Leu Trp Leu Ile Lys Thr Glu Leu Leu Glu Thr Gln Thr Val Asp Phe 450 455 460 Ser Val Gly Ala Glu Gly Leu Arg His Val Pro Gly Asp Val Ile Glu 465 470 475 480 Ile Cys Asp Asp Asp Tyr Ala Gly Ile Ser Thr Gly Gly Arg Val Leu 485 490 495 Ala Val Asn Ser Gln Thr Arg Thr Leu Thr Leu Asp Arg Glu Ile Thr 500 505 510 Leu Pro Ser Ser Gly Thr Ala Leu Ile Ser Leu Val Asp Gly Ser Gly 515 520 525 Asn Pro Val Ser Val Glu Val Gln Ser Val Thr Asp Gly Val Lys Val 530 535 540 Lys Val Ser Arg Val Pro Asp Gly Val Ala Glu Tyr Ser Val Trp Glu 545 550 555 560 Leu Lys Leu Pro Thr Leu Arg Gln Arg Leu Phe Arg Cys Val Ser Ile 565 570 575 Arg Glu Asn Asp Asp Gly Thr Tyr Ala Ile Thr Ala Val Gln His Val 580 585 590 Pro Glu Lys Glu Ala Ile Val Asp Asn Gly Ala His Phe Asp Gly Glu 595 600 605 Gln Ser Gly Thr Val Asn Gly Val Thr Pro Pro Ala Val Gln His Leu 610 615 620 Thr Ala Glu Val Thr Ala Asp Ser Gly Glu Tyr Gln Val Leu Ala Arg 625 630 635 640 Trp Asp Thr Pro Lys Val Val Lys Gly Val Ser Phe Leu Leu Arg Leu 645 650 655 Thr Val Thr Ala Asp Asp Gly Ser Glu Arg Leu Val Ser Thr Ala Arg 660 665 670 Thr Thr Glu Thr Thr Tyr Arg Phe Thr Gln Leu Ala Leu Gly Asn Tyr 675 680 685 Arg Leu Thr Val Arg Ala Val Asn Ala Trp Gly Gln Gln Gly Asp Pro 690 695 700 Ala Ser Val Ser Phe Arg Ile Ala Ala Pro Ala Ala Pro Ser Arg Ile 705 710 715 720 Glu Leu Thr Pro Gly Tyr Phe Gln Ile Thr Ala Thr Pro His Leu Ala 725 730 735 Val Tyr Asp Pro Thr Val Gln Phe Glu Phe Trp Phe Ser Glu Lys Gln 740 745 750 Ile Ala Asp Ile Arg Gln Val Glu Thr Ser Thr Arg Tyr Leu Gly Thr 755 760 765 Ala Leu Tyr Trp Ile Ala Ala Ser Ile Asn Ile Lys Pro Gly His Asp 770 775 780 Tyr Tyr Phe Tyr Ile Arg Ser Val Asn Thr Val Gly Lys Ser Ala Phe 785 790 795 800 Val Glu Ala Val Gly Arg Ala Ser Asp Asp Ala Glu Gly Tyr Leu Asp 805 810 815 Phe Phe Lys Gly Lys Ile Thr Glu Ser His Leu Gly Lys Glu Leu Leu 820 825 830 Glu Lys Val Glu Leu Thr Glu Asp Asn Ala Ser Arg Leu Glu Glu Phe 835 840 845 Ser Lys Glu Trp Lys Asp Ala Ser Asp Lys Trp Asn Ala Met Trp Ala 850 855 860 Val Lys Ile Glu Gln Thr Lys Asp Gly Lys His Tyr Val Ala Gly Ile 865 870 875 880 Gly Leu Ser Met Glu Asp Thr Glu Glu Gly Lys Leu Ser Gln Phe Leu 885 890 895 Val Ala Ala Asn Arg Ile Ala Phe Ile Asp Pro Ala Asn Gly Asn Glu 900 905 910 Thr Pro Met Phe Val Ala Gln Gly Asn Gln Ile Phe Met Asn Asp Val 915 920 925 Phe Leu Lys Arg Leu Thr Ala Pro Thr Ile Thr Ser Gly Gly Asn Pro 930 935 940 Pro Ala Phe Ser Leu Thr Pro Asp Gly Lys Leu Thr Ala Lys Asn Ala 945 950 955 960 Asp Ile Ser Gly Asn Val Asn Ala Asn Ser Gly Thr Leu Asn Asn Val 965 970 975 Thr Ile Asn Glu Asn Cys Gln Ile Lys Gly Lys Leu Ser Ala Asn Gln 980 985 990 Ile Glu Gly Asp Ile Val Lys Thr Val Ser Lys Ser Phe Pro Arg Thr 995 1000 1005 Asn Ser Tyr Ala Ser Gly Thr Ile Thr Val Arg Ile Ser Asp Asp Gln 1010 1015 1020 Lys Phe Asp Arg Gln Val Met Ile Pro Val Leu Phe Arg Gly Gly 1025 1030 1035 1040 Lys His Glu Asn Phe Asn Ser Asn Asn Gln Gln Ser Tyr Trp Tyr Ser 1045 1050 1055 Thr Cys Arg Leu Arg Val Thr Arg Asn Gly Gln Glu Ile Phe Asn Gln 1060 1065 1070 Ser Thr Thr Asp Ala Gin Gly Val Phe Ser Ser Val Ile Asp Met Pro 1075 1080 1085 Ala Gly Gln Gly Thr Leu Thr Leu Thr Phe Thr Val Ser Ser Ser Gly 1090 1095 1100 Ala Asn Asn Trp Thr Pro Thr Thr Ser Ile Ser Asp Leu Leu Val Val 1105 1110 1115 1120 Val Met Lys Lys Ser Thr Ala Gly Ile Ser Ile Ser 1125 1130 <210> 12 <211> 1159 <212> PRT <213> Artificial Sequence <220> <223> gpJ Z2145 <400> 12 Met Gly Lys Gly Ser Ser Lys Gly His Thr Pro Arg Glu Ala Lys Asp 1 5 10 15 Asn Leu Lys Ser Thr Gln Leu Leu Ser Val Ile Asp Ala Ile Ser Glu 20 25 30 Gly Pro Ile Glu Gly Pro Val Asp Gly Leu Lys Ser Val Leu Leu Asn 35 40 45 Ser Thr Pro Val Leu Asp Thr Glu Gly Asn Thr Asn Ile Ser Gly Val 50 55 60 Thr Val Val Phe Arg Ala Gly Glu Gln Glu Gln Thr Pro Pro Glu Gly 65 70 75 80 Phe Glu Ser Ser Gly Ser Glu Thr Val Leu Gly Thr Glu Val Lys Tyr 85 90 95 Asp Thr Pro Ile Thr Arg Thr Ile Thr Ser Ala Asn Ile Asp Arg Leu 100 105 110 Arg Phe Thr Phe Gly Val Gln Ala Leu Val Glu Thr Thr Ser Lys Gly 115 120 125 Asp Arg Asn Pro Ser Glu Val Arg Leu Leu Val Gln Ile Gln Arg Asn 130 135 140 Gly Gly Trp Val Thr Glu Lys Asp Ile Thr Ile Lys Gly Lys Thr Thr 145 150 155 160 Ser Gln Tyr Leu Ala Ser Val Val Met Gly Asn Leu Pro Pro Arg Pro 165 170 175 Phe Asn Ile Arg Met Arg Arg Met Thr Pro Asp Ser Thr Thr Asp Gln 180 185 190 Leu Gln Asn Lys Thr Leu Trp Ser Ser Tyr Thr Glu Ile Ile Asp Val 195 200 205 Lys Gln Cys Tyr Pro Asn Thr Ala Leu Val Gly Val Gln Val Asp Ser 210 215 220 Glu Gln Phe Gly Ser Gln Gln Val Ser Arg Asn Tyr His Leu Arg Gly 225 230 235 240 Arg Ile Leu Gln Val Pro Ser Asn Tyr Asn Pro Gln Thr Arg Gln Tyr 245 250 255 Ser Gly Ile Trp Asp Gly Thr Phe Lys Pro Ala Tyr Ser Asn Asn Met 260 265 270 Ala Trp Cys Leu Trp Asp Met Leu Thr His Pro Arg Tyr Gly Met Gly 275 280 285 Lys Arg Leu Gly Ala Ala Asp Val Asp Lys Trp Ala Leu Tyr Val Ile 290 295 300 Gly Gln Tyr Cys Asp Gln Ser Val Pro Asp Gly Phe Gly Gly Thr Glu 305 310 315 320 Pro Arg Ile Thr Cys Asn Ala Tyr Leu Thr Thr Gln Arg Lys Ala Trp 325 330 335 Asp Val Leu Ser Asp Phe Cys Ser Ala Met Arg Cys Met Pro Val Trp 340 345 350 Asn Gly Gln Thr Leu Thr Phe Val Gln Asp Arg Pro Ser Asp Lys Thr 355 360 365 Trp Thr Tyr Asn Arg Ser Asn Val Val Met Pro Asp Asp Gly Ala Pro 370 375 380 Phe Arg Tyr Ser Phe Ser Ala Leu Lys Asp Arg His Asn Ala Val Glu 385 390 395 400 Val Asn Trp Ile Asp Pro Asn Asn Gly Trp Glu Thr Ala Thr Glu Leu 405 410 415 Val Glu Asp Thr Gln Ala Ile Ala Arg Tyr Gly Arg Asn Val Thr Lys 420 425 430 Met Asp Ala Phe Gly Cys Thr Ser Arg Gly Gln Ala His Arg Ala Gly 435 440 445 Leu Trp Leu Ile Lys Thr Glu Leu Leu Glu Thr Gln Thr Val Asp Phe 450 455 460 Ser Val Gly Ala Glu Gly Leu Arg His Val Pro Gly Asp Val Ile Glu 465 470 475 480 Ile Cys Asp Asp Asp Tyr Ala Gly Ile Ser Thr Gly Gly Arg Val Leu 485 490 495 Ala Val Asn Ser Gln Thr Arg Thr Leu Thr Leu Asp Arg Glu Ile Thr 500 505 510 Leu Pro Ser Ser Gly Thr Ala Leu Ile Ser Leu Val Asp Gly Ser Gly 515 520 525 Asn Pro Val Ser Val Glu Val Gln Ser Val Thr Asp Gly Val Lys Val 530 535 540 Lys Val Ser Arg Val Pro Asp Gly Val Ala Glu Tyr Ser Val Trp Glu 545 550 555 560 Leu Lys Leu Pro Thr Leu Arg Gln Arg Leu Phe Arg Cys Val Ser Ile 565 570 575 Arg Glu Asn Asp Asp Gly Thr Tyr Ala Ile Thr Ala Val Gln His Val 580 585 590 Pro Glu Lys Glu Ala Ile Val Asp Asn Gly Ala His Phe Asp Gly Glu 595 600 605 Gln Ser Gly Thr Val Asn Gly Val Thr Pro Pro Ala Val Gln His Leu 610 615 620 Thr Ala Glu Val Thr Ala Asp Ser Gly Glu Tyr Gln Val Leu Ala Arg 625 630 635 640 Trp Asp Thr Pro Lys Val Val Lys Gly Val Ser Phe Leu Leu Arg Leu 645 650 655 Thr Val Thr Ala Asp Asp Gly Ser Glu Arg Leu Val Ser Thr Ala Arg 660 665 670 Thr Thr Glu Thr Thr Tyr Arg Phe Thr Gln Leu Ala Leu Gly Asn Tyr 675 680 685 Arg Leu Thr Val Arg Ala Val Asn Ala Trp Gly Gln Gln Gly Asp Pro 690 695 700 Ala Ser Val Ser Phe Arg Ile Ala Ala Pro Ala Ala Pro Ser Arg Ile 705 710 715 720 Glu Leu Thr Pro Gly Tyr Phe Gln Ile Thr Ala Thr Pro His Leu Ala 725 730 735 Val Tyr Asp Pro Thr Val Gln Phe Glu Phe Trp Phe Ser Glu Lys Gln 740 745 750 Ile Ala Asp Ile Arg Gln Val Glu Thr Ser Thr Arg Tyr Leu Gly Thr 755 760 765 Ala Leu Tyr Trp Ile Ala Ala Ser Ile Asn Ile Lys Pro Gly His Asp 770 775 780 Tyr Tyr Phe Tyr Ile Arg Ser Val Asn Thr Val Gly Lys Ser Ala Phe 785 790 795 800 Val Glu Ala Val Gly Arg Ala Ser Asp Asp Ala Glu Gly Tyr Leu Asp 805 810 815 Phe Phe Lys Gly Glu Ile Gly Lys Thr His Leu Ala Gln Glu Leu Trp 820 825 830 Thr Gln Ile Asp Asn Gly Gln Leu Ala Pro Asp Leu Ala Glu Ile Arg 835 840 845 Thr Ser Ile Thr Asp Val Ser Asn Glu Ile Thr Gln Thr Val Asn Lys 850 855 860 Lys Leu Glu Asp Gln Ser Ala Ala Ile Gln Gln Ile Gln Lys Val Gln 865 870 875 880 Val Asp Thr Asn Asn Asn Leu Asn Ser Met Trp Ala Val Lys Leu Gln 885 890 895 Gln Met Gln Asp Gly Arg Leu Tyr Ile Ala Gly Ile Gly Ala Gly Ile 900 905 910 Glu Asn Thr Ser Asp Gly Met Gln Ser Gln Val Leu Leu Ala Ala Asp 915 920 925 Arg Ile Ala Met Ile Asn Pro Ala Asn Gly Asn Thr Lys Pro Met Phe 930 935 940 Val Gly Gln Gly Asp Gln Ile Phe Met Asn Glu Val Phe Leu Lys Tyr 945 950 955 960 Leu Thr Ala Pro Thr Ile Thr Ser Gly Gly Asn Pro Pro Ala Phe Ser 965 970 975 Leu Thr Ser Asp Gly Lys Leu Thr Ala Lys Asn Ala Asp Ile Ser Gly 980 985 990 Ser Val Asn Ala Asn Ser Gly Thr Leu Asn Asn Val Thr Ile Asn Glu 995 1000 1005 Asn Cys Arg Val Leu Gly Lys Leu Ser Ala Asn Gln Ile Glu Gly Asp 1010 1015 1020 Leu Val Lys Thr Val Gly Lys Ala Phe Pro Arg Asp Ser Arg Ala Pro 1025 1030 1035 1040 Glu Arg Trp Pro Ser Gly Thr Ile Thr Val Arg Val Tyr Asp Asp Gln 1045 1050 1055 Pro Phe Asp Arg Gln Ile Val Ile Pro Ala Val Ala Phe Ser Gly Ala 1060 1065 1070 Lys His Glu Arg Glu His Thr Asp Ile Tyr Ser Ser Cys Arg Leu Ile 1075 1080 1085 Val Arg Lys Asn Gly Ala Glu Ile Tyr Asn Arg Thr Ala Leu Asp Asn 1090 1095 1100 Thr Leu Ile Tyr Ser Gly Val Ile Asp Met Pro Ala Gly His Gly His 1105 1110 1115 1120 Met Thr Leu Glu Phe Ser Val Ser Ala Trp Leu Val Asn Asn Trp Tyr 1125 1130 1135 Pro Thr Ala Ser Ile Ser Asp Leu Leu Val Val Val Met Lys Lys Ala 1140 1145 1150 Thr Ala Gly Ile Ser Ile Ser 1155 <210> 13 <211> 1131 <212> PRT <213> Artificial Sequence <220> <223> 1A2 <400> 13 Met Gly Lys Gly Ser Ser Lys Gly His Thr Pro Arg Glu Ala Lys Asp 1 5 10 15 Asn Leu Lys Ser Thr Gln Leu Leu Ser Val Ile Asp Ala Ile Ser Glu 20 25 30 Gly Pro Ile Glu Gly Pro Val Asp Gly Leu Lys Ser Val Leu Leu Asn 35 40 45 Ser Thr Pro Val Leu Asp Thr Glu Gly Asn Thr Asn Ile Ser Gly Val 50 55 60 Thr Val Val Phe Arg Ala Gly Glu Gln Glu Gln Thr Pro Pro Glu Gly 65 70 75 80 Phe Glu Ser Ser Gly Ser Glu Thr Val Leu Gly Thr Glu Val Lys Tyr 85 90 95 Asp Thr Pro Ile Thr Arg Thr Ile Thr Ser Ala Asn Ile Asp Arg Leu 100 105 110 Arg Phe Thr Phe Gly Val Gln Ala Leu Val Glu Thr Thr Ser Lys Gly 115 120 125 Asp Arg Asn Pro Ser Glu Val Arg Leu Leu Val Gln Ile Gln Arg Asn 130 135 140 Gly Gly Trp Val Thr Glu Lys Asp Ile Thr Ile Lys Gly Lys Thr Thr 145 150 155 160 Ser Gln Tyr Leu Ala Ser Val Val Met Gly Asn Leu Pro Pro Arg Pro 165 170 175 Phe Asn Ile Arg Met Arg Arg Met Thr Pro Asp Ser Thr Thr Asp Gln 180 185 190 Leu Gln Asn Lys Thr Leu Trp Ser Ser Tyr Thr Glu Ile Ile Asp Val 195 200 205 Lys Gln Cys Tyr Pro Asn Thr Ala Leu Val Gly Val Gln Val Asp Ser 210 215 220 Glu Gln Phe Gly Ser Gln Gln Val Ser Arg Asn Tyr His Leu Arg Gly 225 230 235 240 Arg Ile Leu Gln Val Pro Ser Asn Tyr Asn Pro Gln Thr Arg Gln Tyr 245 250 255 Ser Gly Ile Trp Asp Gly Thr Phe Lys Pro Ala Tyr Ser Asn Asn Met 260 265 270 Ala Trp Cys Leu Trp Asp Met Leu Thr His Pro Arg Tyr Gly Met Gly 275 280 285 Lys Arg Leu Gly Ala Ala Asp Val Asp Lys Trp Ala Leu Tyr Val Ile 290 295 300 Gly Gln Tyr Cys Asp Gln Ser Val Pro Asp Gly Phe Gly Gly Thr Glu 305 310 315 320 Pro Arg Ile Thr Cys Asn Ala Tyr Leu Thr Thr Gln Arg Lys Ala Trp 325 330 335 Asp Val Leu Ser Asp Phe Cys Ser Ala Met Arg Cys Met Pro Val Trp 340 345 350 Asn Gly Gln Thr Leu Thr Phe Val Gln Asp Arg Pro Ser Asp Lys Thr 355 360 365 Trp Thr Tyr Asn Arg Ser Asn Val Val Met Pro Asp Asp Gly Ala Pro 370 375 380 Phe Arg Tyr Ser Phe Ser Ala Leu Lys Asp Arg His Asn Ala Val Glu 385 390 395 400 Val Asn Trp Ile Asp Pro Asn Asn Gly Trp Glu Thr Ala Thr Glu Leu 405 410 415 Val Glu Asp Thr Gln Ala Ile Ala Arg Tyr Gly Arg Asn Val Thr Lys 420 425 430 Met Asp Ala Phe Gly Cys Thr Ser Arg Gly Gln Ala His Arg Ala Gly 435 440 445 Leu Trp Leu Ile Lys Thr Glu Leu Leu Glu Thr Gln Thr Val Asp Phe 450 455 460 Ser Val Gly Ala Glu Gly Leu Arg His Val Pro Gly Asp Val Ile Glu 465 470 475 480 Ile Cys Asp Asp Asp Tyr Ala Gly Ile Ser Thr Gly Gly Arg Val Leu 485 490 495 Ala Val Asn Ser Gln Thr Arg Thr Leu Thr Leu Asp Arg Glu Ile Thr 500 505 510 Leu Pro Ser Ser Gly Thr Ala Leu Ile Ser Leu Val Asp Gly Ser Gly 515 520 525 Asn Pro Val Ser Val Glu Val Gln Ser Val Thr Asp Gly Val Lys Val 530 535 540 Lys Val Ser Arg Val Pro Asp Gly Val Ala Glu Tyr Ser Val Trp Glu 545 550 555 560 Leu Lys Leu Pro Thr Leu Arg Gln Arg Leu Phe Arg Cys Val Ser Ile 565 570 575 Arg Glu Asn Asp Asp Gly Thr Tyr Ala Ile Thr Ala Val Gln His Val 580 585 590 Pro Glu Lys Glu Ala Ile Val Asp Asn Gly Ala His Phe Asp Gly Glu 595 600 605 Gln Ser Gly Thr Val Asn Gly Val Thr Pro Pro Ala Val Gln His Leu 610 615 620 Thr Ala Glu Val Thr Ala Asp Ser Gly Glu Tyr Gln Val Leu Ala Arg 625 630 635 640 Trp Asp Thr Pro Lys Val Val Lys Gly Val Ser Phe Leu Leu Arg Leu 645 650 655 Thr Val Thr Ala Asp Asp Gly Ser Glu Arg Leu Val Ser Thr Ala Arg 660 665 670 Thr Thr Glu Thr Thr Tyr Arg Phe Thr Gln Leu Ala Leu Gly Asn Tyr 675 680 685 Arg Leu Thr Val Arg Ala Val Asn Ala Trp Gly Gln Gln Gly Asp Pro 690 695 700 Ala Ser Val Ser Phe Arg Ile Ala Ala Pro Ala Ala Pro Ser Arg Ile 705 710 715 720 Glu Leu Thr Pro Gly Tyr Phe Gln Ile Thr Ala Thr Pro His Leu Ala 725 730 735 Val Tyr Asp Pro Thr Val Gln Phe Glu Phe Trp Phe Ser Glu Lys Gln 740 745 750 Ile Ala Asp Ile Arg Gln Val Glu Thr Ser Thr Arg Tyr Leu Gly Thr 755 760 765 Ala Leu Tyr Trp Ile Ala Ala Ser Ile Asn Ile Lys Pro Gly His Asp 770 775 780 Tyr Tyr Phe Tyr Ile Arg Ser Val Asn Thr Val Gly Lys Ser Ala Phe 785 790 795 800 Val Glu Ala Val Gly Arg Ala Ser Asp Asp Ala Glu Gly Tyr Leu Asp 805 810 815 Phe Phe Lys Gly Lys Ile Thr Glu Ser His Leu Gly Lys Glu Leu Leu 820 825 830 Glu Lys Val Glu Leu Thr Glu Asp Asn Ala Ser Arg Leu Glu Glu Phe 835 840 845 Ser Lys Glu Trp Lys Asp Ala Ser Asp Lys Trp Asn Ala Met Trp Ala 850 855 860 Val Lys Ile Glu Gln Thr Lys Asp Gly Lys His Tyr Val Ala Gly Ile 865 870 875 880 Gly Leu Ser Met Glu Asp Thr Glu Glu Gly Lys Leu Ser Gln Phe Leu 885 890 895 Val Ala Ala Asn Arg Ile Ala Phe Ile Asp Pro Ala Asn Gly Asn Glu 900 905 910 Thr Pro Met Phe Val Ala Gln Gly Asn Gln Ile Phe Met Asn Asp Val 915 920 925 Phe Leu Lys Arg Leu Thr Ala Pro Thr Ile Thr Ser Gly Gly Asn Pro 930 935 940 Pro Ala Phe Ser Leu Thr Pro Asp Gly Lys Leu Thr Ala Lys Asn Ala 945 950 955 960 Asp Ile Ser Gly Asn Val Asn Ala Asn Ser Gly Thr Leu Asn Asn Val 965 970 975 Thr Ile Asn Glu Asn Cys Arg Val Leu Gly Lys Leu Ser Ala Asn Gln 980 985 990 Ile Glu Gly Asp Leu Val Lys Thr Val Gly Lys Ala Phe Pro Arg Asp 995 1000 1005 Ser Arg Ala Pro Glu Arg Trp Pro Ser Gly Thr Ile Thr Val Arg Val 1010 1015 1020 Tyr Asp Asp Gln Pro Phe Asp Arg Gln Ile Val Ile Pro Ala Val Ala 1025 1030 1035 1040 Phe Ser Gly Ala Lys His Glu Lys Glu His Thr Asp Ile Tyr Ser Ser 1045 1050 1055 Cys Arg Leu Ile Val Arg Lys Asn Gly Ala Glu Ile Tyr Asn Arg Thr 1060 1065 1070 Ala Leu Asp Asn Thr Leu Ile Tyr Ser Gly Val Ile Asp Met Pro Ala 1075 1080 1085 Gly His Gly His Met Thr Leu Glu Phe Ser Val Ser Ala Trp Leu Val 1090 1095 1100 Asn Asn Trp Tyr Pro Thr Ala Ser Ile Ser Asp Leu Leu Val Val Val 1105 1110 1115 1120 Met Lys Lys Ala Thr Ala Gly Ile Thr Ile Ser 1125 1130 <210> 14 <211> 774 <212> PRT <213> Escherichia phage lambda (Bacteriophage lambda) <400> 14 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Ala Gly Asp Ala Ser Ala Ser Ala Ala Gln Val Ala Ala Leu 130 135 140 Val Thr Asp Ala Thr Asp Ser Ala Arg Ala Ala Ser Thr Ser Ala Gly 145 150 155 160 Gln Ala Ala Ser Ser Ala Gln Glu Ala Ser Ser Gly Ala Glu Ala Ala 165 170 175 Ser Ala Lys Ala Thr Glu Ala Glu Lys Ser Ala Ala Ala Ala Glu Ser 180 185 190 Ser Lys Asn Ala Ala Ala Thr Ser Ala Gly Ala Ala Lys Thr Ser Glu 195 200 205 Thr Asn Ala Ala Ala Ser Gln Gln Ser Ala Ala Thr Ser Ala Ser Thr 210 215 220 Ala Ala Thr Lys Ala Ser Glu Ala Ala Thr Ser Ala Arg Asp Ala Val 225 230 235 240 Ala Ser Lys Glu Ala Ala Lys Ser Ser Glu Thr Asn Ala Ser Ser Ser 245 250 255 Ala Gly Arg Ala Ala Ser Ser Ala Thr Ala Ala Glu Asn Ser Ala Arg 260 265 270 Ala Ala Lys Thr Ser Glu Thr Asn Ala Arg Ser Ser Glu Thr Ala Ala 275 280 285 Glu Arg Ser Ala Ser Ala Ala Ala Asp Ala Lys Thr Ala Ala Ala Gly 290 295 300 Ser Ala Ser Thr Ala Ser Thr Lys Ala Thr Glu Ala Ala Gly Ser Ala 305 310 315 320 Val Ser Ala Ser Gln Ser Lys Ser Ala Ala Glu Ala Ala Ala Ile Arg 325 330 335 Ala Lys Asn Ser Ala Lys Arg Ala Glu Asp Ile Ala Ser Ala Val Ala 340 345 350 Leu Glu Asp Ala Asp Thr Thr Arg Lys Gly Ile Val Gln Leu Ser Ser 355 360 365 Ala Thr Asn Ser Thr Ser Glu Thr Leu Ala Ala Thr Pro Lys Ala Val 370 375 380 Lys Val Val Met Asp Glu Thr Asn Arg Lys Ala Pro Leu Asp Ser Pro 385 390 395 400 Ala Leu Thr Gly Thr Pro Thr Ala Pro Thr Ala Leu Arg Gly Thr Asn 405 410 415 Asn Thr Gln Ile Ala Asn Thr Ala Phe Val Leu Ala Ala Ile Ala Asp 420 425 430 Val Ile Asp Ala Ser Pro Asp Ala Leu Asn Thr Leu Asn Glu Leu Ala 435 440 445 Ala Ala Leu Gly Asn Asp Pro Asp Phe Ala Thr Thr Met Thr Asn Ala 450 455 460 Leu Ala Gly Lys Gln Pro Lys Asn Ala Thr Leu Thr Ala Leu Ala Gly 465 470 475 480 Leu Ser Thr Ala Lys Asn Lys Leu Pro Tyr Phe Ala Glu Asn Asp Ala 485 490 495 Ala Ser Leu Thr Glu Leu Thr Gln Val Gly Arg Asp Ile Leu Ala Lys 500 505 510 Asn Ser Val Ala Asp Val Leu Glu Tyr Leu Gly Ala Gly Glu Asn Ser 515 520 525 Ala Phe Pro Ala Gly Ala Pro Ile Pro Trp Pro Ser Asp Ile Val Pro 530 535 540 Ser Gly Tyr Val Leu Met Gln Gly Gln Ala Phe Asp Lys Ser Ala Tyr 545 550 555 560 Pro Lys Leu Ala Val Ala Tyr Pro Ser Gly Val Leu Pro Asp Met Arg 565 570 575 Gly Trp Thr Ile Lys Gly Lys Pro Ala Ser Gly Arg Ala Val Leu Ser 580 585 590 Gln Glu Gln Asp Gly Ile Lys Ser His Thr His Ser Ala Ser Ala Ser 595 600 605 Gly Thr Asp Leu Gly Thr Lys Thr Thr Ser Ser Phe Asp Tyr Gly Thr 610 615 620 Lys Thr Thr Gly Ser Phe Asp Tyr Gly Thr Lys Ser Thr Asn Asn Thr 625 630 635 640 Gly Ala His Ala His Ser Leu Ser Gly Ser Thr Gly Ala Ala Gly Ala 645 650 655 His Ala His Thr Ser Gly Leu Arg Met Asn Ser Ser Gly Trp Ser Gln 660 665 670 Tyr Gly Thr Ala Thr Ile Thr Gly Ser Leu Ser Thr Val Lys Gly Thr 675 680 685 Ser Thr Gln Gly Ile Ala Tyr Leu Ser Lys Thr Asp Ser Gln Gly Ser 690 695 700 His Ser His Ser Leu Ser Gly Thr Ala Val Ser Ala Gly Ala His Ala 705 710 715 720 His Thr Val Gly Ile Gly Ala His Gln His Pro Val Val Ile Gly Ala 725 730 735 His Ala His Ser Phe Ser Ile Gly Ser His Gly His Thr Ile Thr Val 740 745 750 Asn Ala Ala Gly Asn Ala Glu Asn Thr Val Lys Asn Ile Ala Phe Asn 755 760 765 Tyr Ile Val Arg Leu Ala 770 <210> 15 <211> 194 <212> PRT <213> Escherichia phage lambda (Bacteriophage lambda) <400> 15 Met Ala Phe Arg Met Ser Glu Gln Pro Arg Thr Ile Lys Ile Tyr Asn 1 5 10 15 Leu Leu Ala Gly Thr Asn Glu Phe Ile Gly Glu Gly Asp Ala Tyr Ile 20 25 30 Pro Pro His Thr Gly Leu Pro Ala Asn Ser Thr Asp Ile Ala Pro Pro 35 40 45 Asp Ile Pro Ala Gly Phe Val Ala Val Phe Asn Ser Asp Glu Ala Ser 50 55 60 Trp His Leu Val Glu Asp His Arg Gly Lys Thr Val Tyr Asp Val Ala 65 70 75 80 Ser Gly Asp Ala Leu Phe Ile Ser Glu Leu Gly Pro Leu Pro Glu Asn 85 90 95 Phe Thr Trp Leu Ser Pro Gly Gly Glu Tyr Gln Lys Trp Asn Gly Thr 100 105 110 Ala Trp Val Lys Asp Thr Glu Ala Glu Lys Leu Phe Arg Ile Arg Glu 115 120 125 Ala Glu Glu Thr Lys Lys Ser Leu Met Gln Val Ala Ser Glu His Ile 130 135 140 Ala Pro Leu Gln Asp Ala Ala Asp Leu Glu Ile Ala Thr Lys Glu Glu 145 150 155 160 Thr Ser Leu Leu Glu Ala Trp Lys Lys Tyr Arg Val Leu Leu Asn Arg 165 170 175 Val Asp Thr Ser Thr Ala Pro Asp Ile Glu Trp Pro Ala Val Pro Val 180 185 190 Met Glu <210> 16 <211> 1194 <212> PRT <213> Artificial Sequence <220> <223> STF Lambda-WW11.2 <400> 16 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Ala Gly Asp Ala Ser Ala Ser Ala Ala Gln Val Ala Ala Leu 130 135 140 Val Thr Asp Ala Thr Asp Ser Ala Arg Ala Ala Ser Thr Ser Ala Gly 145 150 155 160 Gln Ala Ala Ser Ser Ala Gln Glu Ala Ser Ser Gly Ala Glu Ala Ala 165 170 175 Ser Ala Lys Ala Thr Glu Ala Glu Lys Ser Ala Ala Ala Ala Glu Ser 180 185 190 Ser Lys Asn Ala Ala Ala Thr Ser Ala Gly Ala Ala Lys Thr Ser Glu 195 200 205 Thr Asn Ala Ala Ala Ser Gln Gln Ser Ala Ala Thr Ser Ala Ser Thr 210 215 220 Ala Ala Thr Lys Ala Ser Glu Ala Ala Thr Ser Ala Arg Asp Ala Val 225 230 235 240 Ala Ser Lys Glu Ala Ala Lys Ser Ser Glu Thr Asn Ala Ser Ser Ser 245 250 255 Ala Gly Arg Ala Ala Ser Ser Ala Thr Ala Ala Glu Asn Ser Ala Arg 260 265 270 Ala Ala Lys Thr Ser Glu Thr Asn Ala Arg Ser Ser Glu Thr Ala Ala 275 280 285 Glu Arg Ser Ala Ser Ala Ala Ala Asp Ala Lys Thr Ala Ala Ala Gly 290 295 300 Ser Ala Ser Thr Ala Ser Thr Lys Ala Thr Glu Ala Ala Gly Ser Ala 305 310 315 320 Val Ser Ala Ser Gln Ser Lys Ser Ala Ala Glu Ala Ala Ala Ile Arg 325 330 335 Ala Lys Asn Ser Ala Lys Arg Ala Glu Asp Ile Ala Ser Ala Val Ala 340 345 350 Leu Glu Asp Ala Asp Thr Thr Arg Lys Gly Ile Val Gln Leu Ser Ser 355 360 365 Ala Thr Asn Ser Thr Ser Glu Thr Leu Ala Ala Thr Pro Lys Ala Val 370 375 380 Lys Val Val Met Asp Glu Thr Asn Arg Lys Ala Pro Leu Asp Ser Pro 385 390 395 400 Ala Leu Thr Gly Thr Pro Thr Ala Pro Thr Ala Leu Arg Gly Thr Asn 405 410 415 Asn Thr Gln Ile Ala Asn Thr Ala Phe Val Leu Ala Ala Ile Ala Asp 420 425 430 Val Ile Asp Ala Ser Pro Asp Ala Leu Asn Thr Leu Asn Glu Leu Ala 435 440 445 Ala Ala Leu Gly Asn Asp Pro Asp Phe Ala Thr Thr Met Thr Asn Ala 450 455 460 Leu Ala Gly Lys Gln Pro Lys Asn Ala Thr Leu Thr Ala Leu Ala Gly 465 470 475 480 Leu Ser Thr Ala Lys Asn Lys Leu Pro Tyr Phe Ala Glu Asn Asp Ala 485 490 495 Ala Ser Leu Thr Glu Leu Thr Gln Val Gly Arg Asp Ile Leu Ala Lys 500 505 510 Asn Ser Val Ala Asp Val Leu Glu Tyr Leu Gly Ala Gly Glu Asn Ser 515 520 525 Ala Lys Gln Asp Gly Leu Ser Leu Ile Gly Gln Cys Asn Ser Ile Ala 530 535 540 Asp Leu Arg Asn Val Glu Pro Val Met Asp Gly Gln Arg Ile Leu Val 545 550 555 560 Lys Gln His Thr Ala Gly Thr Gly Lys Gly Gly Gly Thr Phe Gln Ala 565 570 575 Lys Leu Ser Gly Val Gly Leu Thr Asp Asp Asn Gly Val Thr Val Lys 580 585 590 Thr Val Gly Gly Ala Ala Trp Val Arg Val Gly Ala Asp Arg Val Asn 595 600 605 Pro Tyr Met Tyr Gly Ala Leu Gly Ser Gly Asn Asp Asp Thr Leu Ala 610 615 620 Val Gln Ala Cys Leu Asn Ser Gly Lys Ala Thr Asn Ile Thr Asp Val 625 630 635 640 His His Val Ser Asn Val Thr Met Asn Lys Ser Ser Ala Ser Ile Tyr 645 650 655 Gly Ser Gly Leu His Tyr Thr Arg Leu His Gln Leu Pro Ala Ala Val 660 665 670 Gly Ser Leu Leu Thr Ile Ala Asp Thr Cys Ser Leu Thr Val Ile Asp 675 680 685 Ser Leu Gly Leu Tyr Gly Thr Gly Tyr Lys Gln Gly Thr Ala Phe Thr 690 695 700 Thr Gly Thr Arg Gly Ile Asp Val Leu Thr Pro Thr Gly Leu Ser Asn 705 710 715 720 Asn Tyr Pro Asp His Thr Thr Ser Asp Pro Arg Arg Asp Leu Val Ile 725 730 735 Thr Lys Val His Ile Ala Gly Phe Asp Glu Tyr Gly Leu Asn Val Asp 740 745 750 Ser Gly Asn Phe Ser Val Thr Thr Glu Ser Val Leu Ile Asn His Ile 755 760 765 Lys Gln Val Gly Ala Arg Cys Ala Thr Thr Asp Trp Thr Trp Thr Asn 770 775 780 Ile Gln Ile Asn Thr Cys Gly Lys Gln Cys Leu Ile Leu Asp Gly Cys 785 790 795 800 Gly Asn Gly Arg Ile Ile Gly Gly Lys Phe Ile Trp Ala Asn Trp Leu 805 810 815 Pro Tyr Gly Ala Ser Gly Gln Phe Pro Gly Ile Thr Ile Asn Asn Ser 820 825 830 Gln Asn Met Val Ile Asn Gly Ile Glu Val Gln Asp Cys Gly Gly Asn 835 840 845 Gly Ile Glu Phe Thr Asp Ser Tyr Ser Ile Ser Met Asn Gly Leu Asn 850 855 860 Thr Asn Arg Asn Gly Ile Asn Ser Thr Ala Glu Phe Tyr Asn Leu Val 865 870 875 880 Phe Asn Arg Ser Asp Val Glu Ile Asn Gly Phe Val Gly Leu Asn Tyr 885 890 895 Lys Val Asn Ser Gly Ser Pro Asp Asn Ser Ser Ser Gly Asn Leu Lys 900 905 910 Phe Leu Ser Ser Asp Cys Asn Val Thr Leu Asn Gly Lys Leu Glu Thr 915 920 925 Gly Tyr Met Gly Ile Glu Phe Ile Gly Asp Thr Arg Val Ile Gln Pro 930 935 940 Thr Ser Ser Tyr Ile Lys Leu Asn Gly Leu Val Asn Tyr Ala Gly Ser 945 950 955 960 Gly Ile Gln Thr Ile Asn Glu Val Pro Thr Phe Asp Gly Val Ser Thr 965 970 975 Thr Pro Val Tyr Val Pro Ile Ser Ser Val Val Gly Gin Thr Asn Gly 980 985 990 Leu Arg Leu Ser Gln Ala Asn Lys Asp Lys Val Ile Tyr Thr Arg Lys 995 1000 1005 Val Gly Pro Glu Gly Val Thr Met Ala Ala Val Ile Thr Pro Thr Ile 1010 1015 1020 Gly Gly Ala Glu Ile Phe Asn Leu Met Ala Ile Gly Thr Gly Phe Ser 1025 1030 1035 1040 Ser Ala Ser Asn Ser Leu Tyr Phe Gln Leu Glu Ile Ala Ala Asp Gly 1045 1050 1055 Ser Gln Asn Val Ser Leu Leu Leu Ser Gly Asp Gly Thr Thr Gln Ile 1060 1065 1070 Leu Pro Gly Thr Leu Pro Ala Ala Leu Lys Leu Gln Ser Gly Glu Pro 1075 1080 1085 Tyr His Val Ala Leu Gly Ala Lys Ala Gly Tyr Phe Trp Trp Ser Ile 1090 1095 1100 Leu Asn Leu Asn Thr Gly Lys Arg Ile Arg Arg Ser Phe Arg Gly Ala 1105 1110 1115 1120 Tyr Leu Pro Lys Pro Phe Ala Ser Ile Phe Gly Leu Val Ser Ser Pro 1125 1130 1135 Val Phe Phe Ser Gly Ala Gly Ile Gly Asp Ser Gly Cys Ser Gly Val 1140 1145 1150 Gly Ala Lys Ile Tyr Leu Gly Ser Phe Ser Asp Glu Asn Asp Tyr Val 1155 1160 1165 Ser Ser Arg Tyr Tyr Ser Leu Val Asn Pro Val Asp Pro Thr Lys Met 1170 1175 1180 Tyr Ser Phe Arg Ile Leu Asp Ser Thr Ile 1185 1190 <210> 17 <211> 757 <212> PRT <213> Artificial Sequence <220> <223> STF Lambda-75 <400> 17 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Ala Gly Asp Ala Ser Ala Ser Ala Ala Gln Val Ala Ala Leu 130 135 140 Val Thr Asp Ala Thr Asp Ser Ala Arg Ala Ala Ser Thr Ser Ala Gly 145 150 155 160 Gln Ala Ala Ser Ser Ala Gln Glu Ala Ser Ser Gly Ala Glu Ala Ala 165 170 175 Ser Ala Lys Ala Thr Glu Ala Glu Lys Ser Ala Ala Ala Ala Glu Ser 180 185 190 Ser Lys Asn Ala Ala Ala Thr Ser Ala Gly Ala Ala Lys Thr Ser Glu 195 200 205 Thr Asn Ala Ala Ala Ser Gln Gln Ser Ala Ala Thr Ser Ala Ser Thr 210 215 220 Ala Ala Thr Lys Ala Ser Glu Ala Ala Thr Ser Ala Arg Asp Ala Val 225 230 235 240 Ala Ser Lys Glu Ala Ala Lys Ser Ser Glu Thr Asn Ala Ser Ser Ser 245 250 255 Ala Gly Arg Ala Ala Ser Ser Ala Thr Ala Ala Glu Asn Ser Ala Arg 260 265 270 Ala Ala Lys Thr Ser Glu Thr Asn Ala Arg Ser Ser Glu Thr Ala Ala 275 280 285 Glu Arg Ser Ala Ser Ala Ala Ala Asp Ala Lys Thr Ala Ala Ala Gly 290 295 300 Ser Ala Ser Thr Ala Ser Thr Lys Ala Thr Glu Ala Ala Gly Ser Ala 305 310 315 320 Val Ser Ala Ser Gln Ser Lys Ser Ala Ala Glu Ala Ala Ala Ile Arg 325 330 335 Ala Lys Asn Ser Ala Lys Arg Ala Glu Asp Ile Ala Ser Ala Val Ala 340 345 350 Leu Glu Asp Ala Asp Thr Thr Arg Lys Gly Ile Val Gln Leu Ser Ser 355 360 365 Ala Thr Asn Ser Thr Ser Glu Thr Leu Ala Ala Thr Pro Lys Ala Val 370 375 380 Lys Val Val Met Asp Glu Thr Asn Arg Lys Ala Pro Leu Asn Ser Pro 385 390 395 400 Ala Leu Thr Gly Thr Pro Thr Thr Pro Thr Ala Arg Gln Gly Thr Asn 405 410 415 Asn Thr Gln Ile Ala Ser Thr Ala Phe Val Met Ala Ala Ile Ala Ala 420 425 430 Leu Val Asp Ser Ser Pro Asp Ala Leu Asn Thr Leu Asn Glu Leu Ala 435 440 445 Ala Ala Leu Gly Asn Asp Pro Asn Phe Ala Thr Thr Met Thr Asn Ala 450 455 460 Leu Ala Gly Lys Gln Pro Lys Asp Ala Thr Leu Thr Ala Leu Ala Gly 465 470 475 480 Leu Ala Thr Ala Ala Asp Arg Phe Pro Tyr Phe Thr Gly Asn Asp Val 485 490 495 Ala Ser Leu Ala Thr Leu Thr Lys Val Gly Arg Asp Ile Leu Ala Lys 500 505 510 Ser Thr Val Ala Ala Val Ile Glu Tyr Leu Gly Leu Arg Glu Leu Gly 515 520 525 Thr Ser Gly Glu Lys Ile Pro Leu Leu Ser Thr Ala Asn Thr Trp Thr 530 535 540 Asn Arg Gln Thr Leu Ser Gly Gly Leu Ser Gly Glu Leu Ser Gly Asn 545 550 555 560 Ala Ala Thr Ala Thr Lys Leu Lys Thr Ala Arg Lys Ile Ala Gly Val 565 570 575 Gly Phe Asp Gly Ser Ser Asp Ile Ser Ile Ser Ala Lys Asn Val Asn 580 585 590 Ala Phe Ala Leu Arg Gln Thr Gly Asn Thr Val Asn Gly Asp Thr Ser 595 600 605 Val Gly Trp Asn Trp Asp Ser Gly Ala Tyr Asn Ala Leu Ile Gly Gly 610 615 620 Ala Ser Ala Leu Ile Leu His Phe Asn Ile Asn Ala Gly Ser Cys Pro 625 630 635 640 Ala Val Gln Phe Arg Val Asn Tyr Lys Asn Gly Gly Ile Ser Tyr Arg 645 650 655 Ser Ala Arg Asp Gly Tyr Gly Phe Glu Leu Gly Trp Ser Asp Phe Tyr 660 665 670 Thr Thr Thr Arg Lys Pro Ser Ala Gly Asp Val Gly Ala Tyr Thr Gln 675 680 685 Ala Glu Cys Asn Ser Arg Phe Ile Thr Gly Ile Arg Leu Gly Gly Leu 690 695 700 Ser Ser Val Gln Thr Trp Asn Gly Pro Gly Trp Ser Asp Arg Ser Gly 705 710 715 720 Tyr Val Val Thr Gly Ser Val Asn Gly Asn Arg Asp Glu Leu Ile Asp 725 730 735 Thr Thr Gln Ala Arg Pro Ile Gln Tyr Cys Ile Asn Gly Thr Trp Tyr 740 745 750 Asn Ala Gly Ser Ile 755 <210> 18 <211> 177 <212> PRT <213> Artificial Sequence <220> <223> Accessory protein of Lambda-STF75 <400> 18 Met Met His Leu Lys Asn Ile Ile Ala Gly Asn Pro Lys Thr Lys Glu 1 5 10 15 Gln Tyr Gln Leu Thr Lys Gln Phe Asn Ile Lys Trp Leu Tyr Ser Asp 20 25 30 Asp Gly Lys Asn Trp Tyr Glu Glu Gln Lys Asn Phe Gln Pro Asp Thr 35 40 45 Leu Lys Met Val Tyr Asp His Asn Gly Val Ile Ile Cys Ile Glu Lys 50 55 60 Asp Val Ser Ala Ile Asn Pro Glu Gly Ala Ser Val Val Glu Leu Pro 65 70 75 80 Asp Ile Thr Ala Asn Arg Arg Ala Asp Ile Ser Gly Lys Trp Met Phe 85 90 95 Lys Asp Gly Val Val Ile Lys Arg Thr Tyr Thr Glu Glu Glu Gln Arg 100 105 110 Gln Gln Ala Glu Asn Glu Lys Gln Ser Leu Leu Gln Leu Val Arg Asp 115 120 125 Lys Thr Gln Leu Trp Asp Ser Gln Leu Arg Leu Gly Ile Ile Ser Asp 130 135 140 Glu Asn Lys Gln Lys Leu Thr Glu Trp Met Leu Phe Ala Gln Lys Val 145 150 155 160 Glu Ser Thr Asp Thr Ser Ser Leu Pro Val Thr Tyr Pro Glu Gln Pro 165 170 175 Glu <210> 19 <211> 745 <212> PRT <213> Artificial Sequence <220> <223> STF Lambda-EB6 <400> 19 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Ala Gly Asp Ala Ser Ala Ser Ala Ala Gln Val Ala Ala Leu 130 135 140 Val Thr Asp Ala Thr Asp Ser Ala Arg Ala Ala Ser Thr Ser Ala Gly 145 150 155 160 Gln Ala Ala Ser Ser Ala Gln Glu Ala Ser Ser Gly Ala Glu Ala Ala 165 170 175 Ser Ala Lys Ala Thr Glu Ala Glu Lys Ser Ala Ala Ala Ala Glu Ser 180 185 190 Ser Lys Asn Ala Ala Ala Thr Ser Ala Gly Ala Ala Lys Thr Ser Glu 195 200 205 Thr Asn Ala Ala Ala Ser Gln Gln Ser Ala Ala Thr Ser Ala Ser Thr 210 215 220 Ala Ala Thr Lys Ala Ser Glu Ala Ala Thr Ser Ala Arg Asp Ala Val 225 230 235 240 Ala Ser Lys Glu Ala Ala Lys Ser Ser Glu Thr Asn Ala Ser Ser Ser 245 250 255 Ala Gly Arg Ala Ala Ser Ser Ala Thr Ala Ala Glu Asn Ser Ala Arg 260 265 270 Ala Ala Lys Thr Ser Glu Thr Asn Ala Arg Ser Ser Glu Thr Ala Ala 275 280 285 Glu Arg Ser Ala Ser Ala Ala Ala Asp Ala Lys Thr Ala Ala Ala Gly 290 295 300 Ser Ala Ser Thr Ala Ser Thr Lys Ala Thr Glu Ala Ala Gly Ser Ala 305 310 315 320 Val Ser Ala Ser Gln Ser Lys Ser Ala Ala Glu Ala Ala Ala Ile Arg 325 330 335 Ala Lys Asn Ser Ala Lys Arg Ala Glu Asp Ile Ala Ser Ala Val Ala 340 345 350 Leu Glu Asp Ala Asp Thr Thr Arg Lys Gly Ile Val Gln Leu Ser Ser 355 360 365 Ala Thr Asn Ser Thr Ser Glu Thr Leu Ala Ala Thr Pro Lys Ala Val 370 375 380 Lys Ile Ala Met Asp Asn Ala Asn Ala Arg Leu Ala Lys Asp Arg Asn 385 390 395 400 Gly Ala Asp Ile Pro Asn Lys Pro Leu Phe Ile Gln Asn Leu Gly Leu 405 410 415 Gln Glu Thr Val Asn Lys Ala Gly Asn Ala Val Gln Lys Thr Gly Asp 420 425 430 Thr Leu Ser Gly Gly Leu Thr Phe Glu Asn Asp Ser Ile Leu Ala Trp 435 440 445 Ile Arg Asn Thr Asp Trp Ala Lys Ile Gly Phe Lys Asn Asp Ala Asp 450 455 460 Ser Asp Thr Asp Ser Tyr Met Trp Phe Glu Thr Gly Asp Asn Gly Asn 465 470 475 480 Glu Tyr Phe Lys Trp Arg Ser Arg Gln Ser Thr Thr Thr Lys Asp Leu 485 490 495 Met Asn Leu Lys Trp Asp Ala Leu Tyr Val Leu Val Asn Ala Ile Val 500 505 510 Asn Gly Glu Val Ile Ser Lys Ser Ala Asn Gly Leu Arg Ile Ala Tyr 515 520 525 Gly Asn Tyr Gly Phe Phe Ile Arg Asn Asp Gly Ser Asn Thr Tyr Phe 530 535 540 Met Leu Thr Asn Ser Gly Asp Asn Met Gly Thr Tyr Asn Gly Leu Arg 545 550 555 560 Pro Leu Trp Ile Asn Asn Ala Thr Gly Ala Val Ser Met Gly Arg Gly 565 570 575 Leu Asn Val Ser Gly Glu Thr Leu Ser Asp Arg Phe Ala Ile Asn Ser 580 585 590 Ser Asn Gly Met Trp Ile Gln Met Arg Asp Asn Asn Ala Ile Phe Gly 595 600 605 Lys Asn Ile Val Asn Thr Asp Ser Ala Gln Ala Leu Leu Arg Gln Asn 610 615 620 His Ala Asp Arg Lys Phe Met Ile Gly Gly Leu Gly Asn Lys Gln Phe 625 630 635 640 Gly Ile Tyr Met Ile Asn Asn Ser Arg Thr Ala Asn Gly Thr Asp Gly 645 650 655 Gln Ala Tyr Met Asp Asn Asn Gly Asn Trp Leu Cys Gly Ala Gln Val 660 665 670 Ile Pro Gly Asn Tyr Gly Asn Phe Asp Ser Arg Tyr Val Arg Asp Val 675 680 685 Arg Leu Gly Thr Arg Val Val Gln Leu Met Ala Arg Gly Gly Arg Tyr 690 695 700 Glu Lys Ala Gly His Ala Ile Thr Gly Leu Arg Ile Ile Gly Glu Val 705 710 715 720 Asp Gly Asp Asp Glu Ala Ile Phe Arg Pro Ile Gln Lys Tyr Ile Asn 725 730 735 Gly Thr Trp Tyr Asn Val Ala Gln Val 740 745 <210> 20 <211> 175 <212> PRT <213> Artificial Sequence <220> <223> Accessory protein of Lambda-EB6 <400> 20 Met Gln His Leu Lys Asn Ile Lys Ser Gly Asn Pro Lys Thr Lys Glu 1 5 10 15 Gln Tyr Gln Leu Thr Lys Asn Phe Asp Val Ile Trp Leu Trp Ser Glu 20 25 30 Asp Gly Lys Asn Trp Tyr Glu Glu Val Asn Asn Phe Gln Asp Asp Thr 35 40 45 Ile Lys Ile Val Tyr Asp Glu Asn Asn Ile Ile Val Ala Ile Thr Lys 50 55 60 Asp Ala Ser Thr Leu Asn Pro Glu Gly Phe Ser Val Val Glu Ile Pro 65 70 75 80 Asp Ile Thr Ala Asn Arg Arg Ala Asp Asp Ser Gly Lys Trp Met Phe 85 90 95 Lys Asp Gly Ala Val Val Lys Arg Ile Tyr Thr Ala Asp Glu Gln Gln 100 105 110 Gln Gln Ala Glu Ser Gln Lys Ala Ala Leu Leu Ser Glu Ala Glu Asn 115 120 125 Val Ile Gln Pro Leu Glu Arg Ala Val Arg Leu Asn Met Ala Thr Asp 130 135 140 Glu Glu Arg Ala Arg Leu Glu Ser Trp Glu Arg Tyr Ser Val Leu Val 145 150 155 160 Ser Arg Val Asp Thr Ala Lys Pro Glu Trp Pro Gln Lys Pro Glu 165 170 175 <210> 21 <211> 757 <212> PRT <213> Artificial Sequence <220> <223> STF Lambda-23 <400> 21 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Ala Gly Asp Ala Ser Ala Ser Ala Ala Gln Val Ala Ala Leu 130 135 140 Val Thr Asp Ala Thr Asp Ser Ala Arg Ala Ala Ser Thr Ser Ala Gly 145 150 155 160 Gln Ala Ala Ser Ser Ala Gln Glu Ala Ser Ser Gly Ala Glu Ala Ala 165 170 175 Ser Ala Lys Ala Thr Glu Ala Glu Lys Ser Ala Ala Ala Ala Glu Ser 180 185 190 Ser Lys Asn Ala Ala Ala Thr Ser Ala Gly Ala Ala Lys Thr Ser Glu 195 200 205 Thr Asn Ala Ala Ala Ser Gln Gln Ser Ala Ala Thr Ser Ala Ser Thr 210 215 220 Ala Ala Thr Lys Ala Ser Glu Ala Ala Thr Ser Ala Arg Asp Ala Val 225 230 235 240 Ala Ser Lys Glu Ala Ala Lys Ser Ser Glu Thr Asn Ala Ser Ser Ser 245 250 255 Ala Gly Arg Ala Ala Ser Ser Ala Thr Ala Ala Glu Asn Ser Ala Arg 260 265 270 Ala Ala Lys Thr Ser Glu Thr Asn Ala Arg Ser Ser Glu Thr Ala Ala 275 280 285 Glu Arg Ser Ala Ser Ala Ala Ala Asp Ala Lys Thr Ala Ala Ala Gly 290 295 300 Ser Ala Ser Thr Ala Ser Thr Lys Ala Thr Glu Ala Ala Gly Ser Ala 305 310 315 320 Val Ser Ala Ser Gln Ser Lys Ser Ala Ala Glu Ala Ala Ala Ile Arg 325 330 335 Ala Lys Asn Ser Ala Lys Arg Ala Glu Asp Ile Ala Ser Ala Val Ala 340 345 350 Leu Glu Asp Ala Asp Thr Thr Arg Lys Gly Ile Val Gln Leu Ser Ser 355 360 365 Ala Thr Asn Ser Thr Ser Glu Thr Leu Ala Ala Thr Pro Lys Ala Val 370 375 380 Lys Val Val Met Asp Glu Thr Asn Arg Lys Ala Pro Leu Asn Ser Pro 385 390 395 400 Ala Leu Thr Gly Thr Pro Thr Thr Pro Thr Ala Arg Gln Gly Thr Asn 405 410 415 Asn Thr Gln Ile Ala Ser Thr Ala Phe Val Met Ala Ala Ile Ala Ala 420 425 430 Leu Val Asp Ser Ser Pro Asp Ala Leu Asn Thr Leu Asn Glu Leu Ala 435 440 445 Ala Ala Leu Gly Asn Asp Pro Asn Phe Ala Thr Thr Met Thr Asn Ala 450 455 460 Leu Ala Gly Lys Gln Pro Lys Asp Ala Thr Leu Thr Ala Leu Ala Gly 465 470 475 480 Leu Ala Thr Ala Ala Asp Arg Phe Pro Tyr Phe Thr Gly Asn Asp Val 485 490 495 Ala Ser Leu Ala Thr Leu Thr Lys Val Gly Arg Asp Ile Leu Ala Lys 500 505 510 Ser Thr Val Ala Ala Val Ile Glu Tyr Leu Gly Leu Arg Glu Leu Gly 515 520 525 Thr Ser Gly Glu Lys Ile Pro Leu Leu Ser Thr Ala Asn Thr Trp Thr 530 535 540 Asn Arg Gln Thr Phe Ser Gly Gly Leu Ser Gly Gly Leu Ser Gly Asn 545 550 555 560 Ala Ala Thr Ala Thr Lys Leu Lys Thr Ala Arg Lys Ile Ala Gly Val 565 570 575 Gly Phe Asp Gly Ser Ser Asp Ile Ser Ile Ser Ala Lys Asn Val Asn 580 585 590 Ala Phe Ala Leu Arg Gln Thr Gly Asn Thr Val Asn Gly Asp Thr Ser 595 600 605 Val Gly Trp Asn Trp Asp Ser Gly Ala Tyr Asn Ala Leu Ile Gly Gly 610 615 620 Ala Ser Ala Leu Ile Leu His Phe Asn Ile Asn Ala Gly Ser Cys Pro 625 630 635 640 Ala Val Gln Phe Arg Val Asn Tyr Lys Asn Gly Gly Ile Ser Tyr Arg 645 650 655 Ser Ala Arg Asp Gly Tyr Gly Phe Glu Leu Gly Trp Ser Asp Phe Tyr 660 665 670 Thr Thr Thr Arg Lys Pro Ser Ala Gly Asp Val Gly Ala Tyr Thr Arg 675 680 685 Ala Glu Cys Asn Ser Arg Phe Ile Thr Gly Ile Arg Leu Gly Gly Leu 690 695 700 Ser Ser Val Gln Thr Trp Asn Gly Pro Gly Trp Ser Asp Arg Ser Gly 705 710 715 720 Tyr Val Val Thr Gly Ser Val Asn Gly Asn Arg Asp Glu Leu Ile Asp 725 730 735 Thr Thr Gln Ala Arg Pro Ile Gln Tyr Cys Ile Asn Gly Thr Trp Tyr 740 745 750 Asn Ala Gly Ser Ile 755 <210> 22 <211> 177 <212> PRT <213> Artificial Sequence <220> <223> Accessory protein of Lambda-STF23 <400> 22 Met Met His Leu Lys Asn Ile Thr Ala Gly Asn Pro Lys Thr Lys Glu 1 5 10 15 Gln Tyr Gln Leu Thr Lys Gln Phe Asn Ile Lys Trp Leu Tyr Ser Asp 20 25 30 Asp Gly Lys Asn Trp Tyr Glu Glu Gln Lys Asn Phe Gln Pro Asp Thr 35 40 45 Leu Lys Met Val Tyr Asp His Asn Gly Val Ile Ile Cys Ile Glu Lys 50 55 60 Asp Val Ser Ala Ile Asn Pro Glu Gly Ala Ser Val Val Glu Leu Pro 65 70 75 80 Asp Ile Thr Ala Asn Arg Arg Ala Asp Ile Ser Gly Lys Trp Leu Phe 85 90 95 Lys Asp Gly Val Val Ile Lys Arg Thr Tyr Thr Glu Glu Glu Gln Arg 100 105 110 Gln Gln Ala Glu Asn Glu Lys Gln Ser Leu Leu Gln Leu Val Arg Asp 115 120 125 Lys Thr Gln Leu Trp Asp Ser Gln Leu Arg Leu Gly Ile Ile Ser Asp 130 135 140 Glu Asn Lys Gln Lys Leu Thr Glu Trp Met Leu Tyr Ala Gln Lys Val 145 150 155 160 Glu Ser Thr Asp Thr Ser Ser Leu Pro Val Thr Phe Pro Glu Gln Pro 165 170 175 Glu <210> 23 <211> 853 <212> PRT <213> Escherichia phage lambda (Bacteriophage lambda) <220> <221> MISC_FEATURE <222> (187)..(208) <223> distal region box in Figure 9A <220> <221> MISC_FEATURE <222> (301)..(414) <223> proximal region box in Figure 9A <400> 23 Met Ala Glu Pro Val Gly Asp Leu Val Val Asp Leu Ser Leu Asp Ala 1 5 10 15 Ala Arg Phe Asp Glu Gln Met Ala Arg Val Arg Arg His Phe Ser Gly 20 25 30 Thr Glu Ser Asp Ala Lys Lys Thr Ala Ala Val Val Glu Gln Ser Leu 35 40 45 Ser Arg Gln Ala Leu Ala Ala Gln Lys Ala Gly Ile Ser Val Gly Gln 50 55 60 Tyr Lys Ala Ala Met Arg Met Leu Pro Ala Gln Phe Thr Asp Val Ala 65 70 75 80 Thr Gln Leu Ala Gly Gly Gln Ser Pro Trp Leu Ile Leu Leu Gln Gln 85 90 95 Gly Gly Gln Val Lys Asp Ser Phe Gly Gly Met Ile Pro Met Phe Arg 100 105 110 Gly Leu Ala Gly Ala Ile Thr Leu Pro Met Val Gly Ala Thr Ser Leu 115 120 125 Ala Val Ala Thr Gly Ala Leu Ala Tyr Ala Trp Tyr Gln Gly Asn Ser 130 135 140 Thr Leu Ser Asp Phe Asn Lys Thr Leu Val Leu Ser Gly Asn Gln Ala 145 150 155 160 Gly Leu Thr Ala Asp Arg Met Leu Val Leu Ser Arg Ala Gly Gln Ala 165 170 175 Ala Gly Leu Thr Phe Asn Gln Thr Ser Glu Ser Leu Ser Ala Leu Val 180 185 190 Lys Ala Gly Val Ser Gly Glu Ala Gln Ile Ala Ser Ile Ser Gln Ser 195 200 205 Val Ala Arg Phe Ser Ser Ala Ser Gly Val Glu Val Asp Lys Val Ala 210 215 220 Glu Ala Phe Gly Lys Leu Thr Thr Asp Pro Thr Ser Gly Leu Thr Ala 225 230 235 240 Met Ala Arg Gln Phe His Asn Val Ser Ala Glu Gln Ile Ala Tyr Val 245 250 255 Ala Gln Leu Gln Arg Ser Gly Asp Glu Ala Gly Ala Leu Gln Ala Ala 260 265 270 Asn Glu Ala Ala Thr Lys Gly Phe Asp Asp Gln Thr Arg Arg Leu Lys 275 280 285 Glu Asn Met Gly Thr Leu Glu Thr Trp Ala Asp Arg Thr Ala Arg Ala 290 295 300 Phe Lys Ser Met Trp Asp Ala Val Leu Asp Ile Gly Arg Pro Asp Thr 305 310 315 320 Ala Gln Glu Met Leu Ile Lys Ala Glu Ala Ala Tyr Lys Lys Ala Asp 325 330 335 Asp Ile Trp Asn Leu Arg Lys Asp Asp Tyr Phe Val Asn Asp Glu Ala 340 345 350 Arg Ala Arg Tyr Trp Asp Asp Arg Glu Lys Ala Arg Leu Ala Leu Glu 355 360 365 Ala Ala Arg Lys Lys Ala Glu Gln Gln Thr Gln Gln Asp Lys Asn Ala 370 375 380 Gln Gln Gln Ser Asp Thr Glu Ala Ser Arg Leu Lys Tyr Thr Glu Glu 385 390 395 400 Ala Gln Lys Ala Tyr Glu Arg Leu Gln Thr Pro Leu Glu Lys Tyr Thr 405 410 415 Ala Arg Gln Glu Glu Leu Asn Lys Ala Leu Lys Asp Gly Lys Ile Leu 420 425 430 Gln Ala Asp Tyr Asn Thr Leu Met Ala Ala Ala Lys Lys Asp Tyr Glu 435 440 445 Ala Thr Leu Lys Lys Pro Lys Gln Ser Ser Val Lys Val Ser Ala Gly 450 455 460 Asp Arg Gln Glu Asp Ser Ala His Ala Ala Leu Leu Thr Leu Gln Ala 465 470 475 480 Glu Leu Arg Thr Leu Glu Lys His Ala Gly Ala Asn Glu Lys Ile Ser 485 490 495 Gln Gln Arg Arg Asp Leu Trp Lys Ala Glu Ser Gln Phe Ala Val Leu 500 505 510 Glu Glu Ala Ala Gln Arg Arg Gln Leu Ser Ala Gln Glu Lys Ser Leu 515 520 525 Leu Ala His Lys Asp Glu Thr Leu Glu Tyr Lys Arg Gln Leu Ala Ala 530 535 540 Leu Gly Asp Lys Val Thr Tyr Gln Glu Arg Leu Asn Ala Leu Ala Gln 545 550 555 560 Gln Ala Asp Lys Phe Ala Gln Gln Gln Arg Ala Lys Arg Ala Ala Ile 565 570 575 Asp Ala Lys Ser Arg Gly Leu Thr Asp Arg Gln Ala Glu Arg Glu Ala 580 585 590 Thr Glu Gln Arg Leu Lys Glu Gln Tyr Gly Asp Asn Pro Leu Ala Leu 595 600 605 Asn Asn Val Met Ser Glu Gln Lys Lys Thr Trp Ala Ala Glu Asp Gln 610 615 620 Leu Arg Gly Asn Trp Met Ala Gly Leu Lys Ser Gly Trp Ser Glu Trp 625 630 635 640 Glu Glu Ser Ala Thr Asp Ser Met Ser Gln Val Lys Ser Ala Ala Thr 645 650 655 Gln Thr Phe Asp Gly Ile Ala Gln Asn Met Ala Ala Met Leu Thr Gly 660 665 670 Ser Glu Gln Asn Trp Arg Ser Phe Thr Arg Ser Val Leu Ser Met Met 675 680 685 Thr Glu Ile Leu Leu Lys Gln Ala Met Val Gly Ile Val Gly Ser Ile 690 695 700 Gly Ser Ala Ile Gly Gly Ala Val Gly Gly Gly Ala Ser Ala Ser Gly 705 710 715 720 Gly Thr Ala Ile Gln Ala Ala Ala Ala Lys Phe His Phe Ala Thr Gly 725 730 735 Gly Phe Thr Gly Thr Gly Gly Lys Tyr Glu Pro Ala Gly Ile Val His 740 745 750 Arg Gly Glu Phe Val Phe Thr Lys Glu Ala Thr Ser Arg Ile Gly Val 755 760 765 Gly Asn Leu Tyr Arg Leu Met Arg Gly Tyr Ala Thr Gly Gly Tyr Val 770 775 780 Gly Thr Pro Gly Ser Met Ala Asp Ser Arg Ser Gln Ala Ser Gly Thr 785 790 795 800 Phe Glu Gln Asn Asn His Val Val Ile Asn Asn Asp Gly Thr Asn Gly 805 810 815 Gln Ile Gly Pro Ala Ala Leu Lys Ala Val Tyr Asp Met Ala Arg Lys 820 825 830 Gly Ala Arg Asp Glu Ile Gln Thr Gln Met Arg Asp Gly Gly Leu Phe 835 840 845 Ser Gly Gly Gly Arg 850 <210> 24 <211> 859 <212> PRT <213> Artificial Sequence <220> <223> gpH-IAI <400> 24 Met Ala Glu Pro Val Gly Asp Leu Val Val Asp Leu Ser Leu Asp Ala 1 5 10 15 Ala Arg Phe Asp Glu Gln Met Ala Arg Val Arg Arg His Phe Ser Gly 20 25 30 Thr Glu Ser Asp Ala Lys Lys Thr Ala Ala Val Val Glu Gln Ser Leu 35 40 45 Ser Arg Gln Ala Leu Ala Ala Gln Lys Ala Gly Ile Ser Val Gly Gln 50 55 60 Tyr Lys Ala Ala Met Arg Met Leu Pro Ala Gln Phe Thr Asp Val Ala 65 70 75 80 Thr Gln Leu Ala Gly Gly Gln Ser Pro Trp Leu Ile Leu Leu Gln Gln 85 90 95 Gly Gly Gln Val Lys Asp Ser Phe Gly Gly Met Ile Pro Met Phe Arg 100 105 110 Gly Leu Ala Gly Ala Ile Thr Leu Pro Met Val Gly Ala Thr Ser Leu 115 120 125 Ala Val Ala Thr Gly Ala Leu Ala Tyr Ala Trp Tyr Gln Gly Asn Ser 130 135 140 Thr Leu Ser Asp Phe Asn Lys Thr Leu Val Leu Ser Gly Asn Gln Ala 145 150 155 160 Gly Leu Thr Ala Asp Arg Met Leu Val Leu Ser Arg Ala Gly Gln Ala 165 170 175 Ala Gly Leu Thr Phe Asn Gln Thr Ser Glu Ser Leu Thr Ala Leu Val 180 185 190 Asn Ala Gly Val Arg Gly Gly Glu Gln Phe Glu Ala Ile Ser Gln Ser 195 200 205 Val Ala Arg Phe Ser Ser Ala Ser Gly Val Glu Val Asp Lys Val Ala 210 215 220 Glu Ala Phe Gly Lys Leu Thr Thr Asp Pro Thr Ser Gly Leu Thr Ala 225 230 235 240 Met Ala Arg Gln Phe His Asn Val Thr Ala Glu Gln Ile Ala Tyr Val 245 250 255 Ala Gln Leu Gln Arg Ser Gly Asp Glu Ala Gly Ala Leu Gln Ala Ala 260 265 270 Asn Glu Ala Ala Thr Lys Gly Phe Asp Asp Gln Thr Arg Arg Leu Lys 275 280 285 Glu Asn Met Gly Thr Leu Glu Thr Trp Ala Asp Arg Thr Ala Arg Ala 290 295 300 Phe Lys Ser Met Trp Asp Ser Val Leu Asp Ile Gly Arg Pro Asp Thr 305 310 315 320 Ala Gln Gly Met Leu Glu Lys Ala Glu Lys Ala Phe Asp Glu Ala Asp 325 330 335 Lys Lys Trp Gln Trp Tyr Gln Ser Arg Ser His Arg Arg Gly Lys Thr 340 345 350 Ser Ala Phe Leu Ala Asn Leu Arg Gly Ala Trp Glu Asp Arg Ala Asn 355 360 365 Ala Gln Leu Gly Leu Ser Ala Ala Thr Leu Gln Ala Asp Leu Glu Lys 370 375 380 Ala Arg Glu Met Ala Ala Lys Asp Trp Ala Glu Ser Glu Ala Ser Arg 385 390 395 400 Leu Lys Tyr Thr Glu Glu Ala Gln Lys Ala Tyr Glu Arg Leu Gln Thr 405 410 415 Pro Leu Glu Lys Tyr Thr Ala Arg Gln Glu Glu Leu Asn Lys Ala Leu 420 425 430 Lys Asp Gly Lys Ile Leu Gln Ala Asp Tyr Asn Thr Leu Met Ala Ala 435 440 445 Ala Lys Lys Asp Tyr Glu Ala Thr Leu Lys Lys Pro Lys Gln Ser Ser 450 455 460 Val Lys Val Ser Ala Gly Asp Arg Gln Glu Asp Ser Ala His Ala Ala 465 470 475 480 Leu Leu Thr Leu Gln Ala Glu Leu Arg Thr Leu Glu Lys His Ala Gly 485 490 495 Ala Asn Glu Lys Ile Ser Gln Gln Arg Arg Asp Leu Trp Lys Ala Glu 500 505 510 Ser Gln Phe Ala Val Leu Glu Glu Ala Ala Gln Arg Arg Gln Leu Ser 515 520 525 Ala Gln Glu Lys Ser Leu Leu Ala His Lys Asp Glu Thr Leu Glu Tyr 530 535 540 Lys Arg Gln Leu Ala Ala Leu Gly Asp Lys Val Thr Tyr Gln Glu Arg 545 550 555 560 Leu Asn Ala Leu Ala Gln Gln Ala Asp Lys Phe Ala Gln Gln Gln Arg 565 570 575 Ala Lys Arg Ala Ala Ile Asp Ala Lys Ser Arg Gly Leu Thr Asp Arg 580 585 590 Gln Ala Glu Arg Glu Ala Thr Glu Gln Arg Leu Lys Glu Gln Tyr Gly 595 600 605 Asp Asn Pro Leu Ala Leu Asn Asn Val Met Ser Glu Gln Lys Lys Thr 610 615 620 Trp Ala Ala Glu Asp Gln Leu Arg Gly Asn Trp Met Ala Gly Leu Lys 625 630 635 640 Ser Gly Trp Ser Glu Trp Glu Glu Ser Ala Thr Asp Ser Met Ser Gln 645 650 655 Val Lys Ser Ala Ala Thr Gln Thr Phe Asp Gly Ile Ala Gln Asn Met 660 665 670 Ala Ala Met Leu Thr Gly Ser Glu Gln Asn Trp Arg Ser Phe Thr Arg 675 680 685 Ser Val Leu Ser Met Met Thr Glu Ile Leu Leu Lys Gln Ala Met Val 690 695 700 Gly Ile Val Gly Ser Ile Gly Ser Ala Ile Gly Gly Ala Val Gly Gly 705 710 715 720 Gly Ala Ser Ala Ser Gly Gly Thr Ala Ile Gln Ala Ala Ala Ala Lys 725 730 735 Phe His Phe Ala Thr Gly Gly Phe Thr Gly Thr Gly Gly Lys Tyr Glu 740 745 750 Pro Ala Gly Ile Val His Arg Gly Glu Phe Val Phe Thr Lys Glu Ala 755 760 765 Thr Ser Arg Ile Gly Val Gly Asn Leu Tyr Arg Leu Met Arg Gly Tyr 770 775 780 Ala Thr Gly Gly Tyr Val Gly Thr Pro Gly Ser Met Ala Asp Ser Arg 785 790 795 800 Ser Gln Ala Ser Gly Thr Phe Glu Gln Asn Asn His Val Val Ile Asn 805 810 815 Asn Asp Gly Thr Asn Gly Gln Ile Gly Pro Ala Ala Leu Lys Ala Val 820 825 830 Tyr Asp Met Ala Arg Lys Gly Ala Arg Asp Glu Ile Gln Thr Gln Met 835 840 845 Arg Asp Gly Gly Leu Phe Ser Gly Gly Gly Arg 850 855 <210> 25 <211> 3396 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding H591 <400> 25 atgggtaaag gaagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaagtcc 60 acgcagttgc tgagtgtgat cgatgccatc agcgaagggc cgattgaagg tccggtggat 120 ggcttaaaaa gcgtgctgct gaacagtacg ccggtgctgg acactgaggg gaataccaac 180 atatccggtg tcacggtggt gttccgggct ggtgagcagg agcagactcc gccggaggga 240 tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga cacgccgatc 300 acccgcacca ttacgtctgc aaacatcgac cgtctgcgct ttaccttcgg tgtacaggca 360 ctggtggaaa ccacctcaaa gggtgacagg aatccgtcgg aagtccgcct gctggttcag 420 atacaacgta acggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccacc 480 tcgcagtatc tggcctcggt ggtgatgggt aacctgccgc cgcgcccgtt taatatccgg 540 atgcgcagga tgacgccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg 600 tcatacactg aaatcatcga tgtgaaacag tgctacccga acacggcact ggtcggcgtg 660 caggtggact cggagcagtt cggcagccag caggtgagcc gtaattatca tctgcgcggg 720 cgtattctgc aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg 780 gacggaacgt ttaaaccggc atacagcaac aacatggcct ggtgtctgtg ggatatgctg 840 acccatccgc gctacggcat ggggaaacgt cttggtgcgg cggatgtgga taaatgggcg 900 ctgtatgtca tcggccagta ctgcgaccag tcagtgccgg acggctttgg cggcacggag 960 ccgcgcatca cctgtaatgc gtacctgacc acacagcgta aggcgtggga tgtgctcagc 1020 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg 1080 caggaccgac cgtcggataa gacgtggacc tataaccgca gtaatgtggt gatgccggat 1140 gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa tgccgttgag 1200 gtgaactgga ttgacccgaa caacggctgg gagacggcga cagagcttgt tgaagatacg 1260 caggccattg cccgttacgg tcgtaatgtt acgaagatgg atgcctttgg ctgtaccagc 1320 cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cagaactgct ggaaacgcag 1380 accgtggatt tcagcgtcgg cgcagaaggg cttcgccatg taccgggcga tgttattgaa 1440 atctgcgatg atgactatgc cggtatcagc accggtggtc gtgtgctggc ggtgaacagc 1500 cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg taccgcgctg 1560 ataagcctgg ttgacggaag tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac 1620 ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag cgtatgggag 1680 ctgaagctgc cgacgctgcg ccagcgactg ttccgctgcg tgagtatccg tgagaacgac 1740 gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaggc catcgtggat 1800 aacggggcgc actttgacgg cgaacagagt ggcacggtga atggtgtcac gccgccagcg 1860 gtgcagcacc tgaccgcaga agtcactgca gacagcgggg aatatcaggt gctggcgcga 1920 tgggacacac cgaaggtggt gaagggcgtg agtttcctgc tccgtctgac cgtaacagcg 1980 gacgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac ataccgcttc 2040 acgcaactgg cgctggggaa ctacaggctg acagtccggg cggtaaatgc gtgggggcag 2100 cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcacc gtcgaggatt 2160 gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgccgt ttatgacccg 2220 acggtacagt ttgagttctg gttctcggaa aagcagattg cggatatcag acaggttgaa 2280 accagcacgc gttatcttgg tacggcgctg tactggatag ccgccagtat caatatcaaa 2340 ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa atcggcattc 2400 gtggaggccg tcggtcgggc gagcgatgat gcggaaggtt acctggattt tttcaaaggc 2460 aagataaccg aatcccatct cggcaaggag ctgctggaaa aagtcgagct gacggaggat 2520 aacgccagca gactggagga gttttcgaaa gagtggaagg atgccagtga taagtggaat 2580 gccatgtggg ctgtcaaaat tgagcagacc aaagacggca aacattatgt cgcgggtatt 2640 ggcctcagca tggaggacac ggaggaaggc aaactgagcc agtttctggt tgccgccaat 2700 cgtatcgcat ttattgaccc ggcaaacggg aatgaaacgc cgatgtttgt ggcgcagggc 2760 aaccagatat tcatgaacga cgtgttcctg aagcgcctga cggccccccac cattaccagc 2820 ggcggcaatc ctccggcctt ttccctgaca ccggacggaa agctgaccgc taaaaatgcg 2880 gatatcagtg gcaatgtgaa tgcaaattca gggacgctca acaatgtcac gattaatgaa 2940 aactgtcaga ttaaagggaa actgtcagcc aatcagattg aaggcgatat tgtcaaaacg 3000 gtcagcaagt ctttcccccg cacgaacagt tatgccagtg gcaccatcac ggtaagaatc 3060 agtgatgatc agaaatttga ccggcaggtc atgataccgc cagtgttatt ccgcggtggt 3120 aagcatgaga atttcaacag taataaccaa cagtcatact ggtattcaac ctgccggtta 3180 agagtgaccc gcaatggtca ggagattttt aatcagtcca cgacggatgc tcagggcgta 3240 ttttcctcag ttatagatat gcctgccgga caggggacac tgacactgac attcaccgta 3300 tcttcatcag gagcgaataa ctggacacca acaaccagta tcagcgatct gctggttgtg 3360 gtgatgaaga aatccacagc aggtatcagt atcagc 3396 <210> 26 <211> 3477 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding Z2145 <400> 26 atgggtaaag gaagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaagtcc 60 acgcagttgc tgagtgtgat cgatgccatc agcgaagggc cgattgaagg tccggtggat 120 ggcttaaaaa gcgtgctgct gaacagtacg ccggtgctgg acactgaggg gaataccaac 180 atatccggtg tcacggtggt gttccgggct ggtgagcagg agcagactcc gccggaggga 240 tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga cacgccgatc 300 acccgcacca ttacgtctgc aaacatcgac cgtctgcgct ttaccttcgg tgtacaggca 360 ctggtggaaa ccacctcaaa gggtgacagg aatccgtcgg aagtccgcct gctggttcag 420 atacaacgta acggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccacc 480 tcgcagtatc tggcctcggt ggtgatgggt aacctgccgc cgcgcccgtt taatatccgg 540 atgcgcagga tgacgccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg 600 tcatacactg aaatcatcga tgtgaaacag tgctacccga acacggcact ggtcggcgtg 660 caggtggact cggagcagtt cggcagccag caggtgagcc gtaattatca tctgcgcggg 720 cgtattctgc aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg 780 gacggaacgt ttaaaccggc atacagcaac aacatggcct ggtgtctgtg ggatatgctg 840 acccatccgc gctacggcat ggggaaacgt cttggtgcgg cggatgtgga taaatgggcg 900 ctgtatgtca tcggccagta ctgcgaccag tcagtgccgg acggctttgg cggcacggag 960 ccgcgcatca cctgtaatgc gtacctgacc acacagcgta aggcgtggga tgtgctcagc 1020 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg 1080 caggaccgac cgtcggataa gacgtggacc tataaccgca gtaatgtggt gatgccggat 1140 gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa tgccgttgag 1200 gtgaactgga ttgacccgaa caacggctgg gagacggcga cagagcttgt tgaagatacg 1260 caggccattg cccgttacgg tcgtaatgtt acgaagatgg atgcctttgg ctgtaccagc 1320 cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cagaactgct ggaaacgcag 1380 accgtggatt tcagcgtcgg cgcagaaggg cttcgccatg taccgggcga tgttattgaa 1440 atctgcgatg atgactatgc cggtatcagc accggtggtc gtgtgctggc ggtgaacagc 1500 cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg taccgcgctg 1560 ataagcctgg ttgacggaag tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac 1620 ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag cgtatgggag 1680 ctgaagctgc cgacgctgcg ccagcgactg ttccgctgcg tgagtatccg tgagaacgac 1740 gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaggc catcgtggat 1800 aacggggcgc actttgacgg cgaacagagt ggcacggtga atggtgtcac gccgccagcg 1860 gtgcagcacc tgaccgcaga agtcactgca gacagcgggg aatatcaggt gctggcgcga 1920 tgggacacac cgaaggtggt gaagggcgtg agtttcctgc tccgtctgac cgtaacagcg 1980 gacgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac ataccgcttc 2040 acgcaactgg cgctggggaa ctacaggctg acagtccggg cggtaaatgc gtgggggcag 2100 cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcacc gtcgaggatt 2160 gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgccgt ttatgacccg 2220 acggtacagt ttgagttctg gttctcggaa aagcagattg cggatatcag acaggttgaa 2280 accagcacgc gttatcttgg tacggcgctg tactggatag ccgccagtat caatatcaaa 2340 ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa atcggcattc 2400 gtggaggccg tcggtcgggc gagcgatgat gcggaaggtt acctggattt tttcaaaggc 2460 gagataggga aaacccatct ggctcaggag ttgtggactc agattgataa cggtcagctt 2520 gcgcctgacc tggcggaaat cagaacgtcc atcacggatg tcagtaatga aatcacgcag 2580 accgtcaata agaaactgga agaccagagt gcagcgatcc agcagataca gaaggttcag 2640 gttgatacaa ataataacct gaacagcatg tgggcagtga agctgcagca gatgcaggac 2700 ggacgccttt atattgcggg tatcggtgcc ggtattgaga acacctctga cggcatgcag 2760 agtcaggtgc tgctggcggc agacaggatt gcgatgatta atcctgcgaa tggcaacaca 2820 aagccgatgt ttgttggtca gggcgatcag atattcatga atgaagtgtt cctgaaatat 2880 ctgacggctc ccaccattac cagtggcggc aatcctccgg cattttccct gacatcagac 2940 ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa ctccgggacg 3000 ctcaacaacg tcacgattaa cgagaactgt cgggttctgg gaaaactgtc cgcgaaccag 3060 attgaaggcg atctcgttaa aacagtgggc aaagctttcc cccgggactc ccgtgcaccg 3120 gaacggtggc catcagggac cattaccgtc agggtttatg acgatcagcc gtttgaccgg 3180 cagattgtta ttccggcggt ggcattcagc ggcgctaaac atgagagaga gcatactgat 3240 atttactcct catgccgtct gatagtgcgg aaaaacggtg ctgaaattta taaccgtacc 3300 gcgctggata atacgctgat ttacagtggc gttattgata tgcctgccgg tcacggtcac 3360 atgacgctgg agttttcggt gtcagcatgg ctggtgaata actggtatcc cacagcaagt 3420 atcagcgatt tgctggttgt ggtgatgaag aaagccaccg caggcatcag tatcagc 3477 <210> 27 <211> 3393 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding 1A2 <400> 27 atgggtaaag gaagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaagtcc 60 acgcagttgc tgagtgtgat cgatgccatc agcgaagggc cgattgaagg tccggtggat 120 ggcttaaaaa gcgtgctgct gaacagtacg ccggtgctgg acactgaggg gaataccaac 180 atatccggtg tcacggtggt gttccgggct ggtgagcagg agcagactcc gccggaggga 240 tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga cacgccgatc 300 acccgcacca ttacgtctgc aaacatcgac cgtctgcgct ttaccttcgg tgtacaggca 360 ctggtggaaa ccacctcaaa gggtgacagg aatccgtcgg aagtccgcct gctggttcag 420 atacaacgta acggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccacc 480 tcgcagtatc tggcctcggt ggtgatgggt aacctgccgc cgcgcccgtt taatatccgg 540 atgcgcagga tgacgccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg 600 tcatacactg aaatcatcga tgtgaaacag tgctacccga acacggcact ggtcggcgtg 660 caggtggact cggagcagtt cggcagccag caggtgagcc gtaattatca tctgcgcggg 720 cgtattctgc aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg 780 gacggaacgt ttaaaccggc atacagcaac aacatggcct ggtgtctgtg ggatatgctg 840 acccatccgc gctacggcat ggggaaacgt cttggtgcgg cggatgtgga taaatgggcg 900 ctgtatgtca tcggccagta ctgcgaccag tcagtgccgg acggctttgg cggcacggag 960 ccgcgcatca cctgtaatgc gtacctgacc acacagcgta aggcgtggga tgtgctcagc 1020 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg 1080 caggaccgac cgtcggataa gacgtggacc tataaccgca gtaatgtggt gatgccggat 1140 gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa tgccgttgag 1200 gtgaactgga ttgacccgaa caacggctgg gagacggcga cagagcttgt tgaagatacg 1260 caggccattg cccgttacgg tcgtaatgtt acgaagatgg atgcctttgg ctgtaccagc 1320 cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cagaactgct ggaaacgcag 1380 accgtggatt tcagcgtcgg cgcagaaggg cttcgccatg taccgggcga tgttattgaa 1440 atctgcgatg atgactatgc cggtatcagc accggtggtc gtgtgctggc ggtgaacagc 1500 cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg taccgcgctg 1560 ataagcctgg ttgacggaag tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac 1620 ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag cgtatgggag 1680 ctgaagctgc cgacgctgcg ccagcgactg ttccgctgcg tgagtatccg tgagaacgac 1740 gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaggc catcgtggat 1800 aacggggcgc actttgacgg cgaacagagt ggcacggtga atggtgtcac gccgccagcg 1860 gtgcagcacc tgaccgcaga agtcactgca gacagcgggg aatatcaggt gctggcgcga 1920 tgggacacac cgaaggtggt gaagggcgtg agtttcctgc tccgtctgac cgtaacagcg 1980 gacgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac ataccgcttc 2040 acgcaactgg cgctggggaa ctacaggctg acagtccggg cggtaaatgc gtgggggcag 2100 cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcacc gtcgaggatt 2160 gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgccgt ttatgacccg 2220 acggtacagt ttgagttctg gttctcggaa aagcagattg cggatatcag acaggttgaa 2280 accagcacgc gttatcttgg tacggcgctg tactggatag ccgccagtat caatatcaaa 2340 ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa atcggcattc 2400 gtggaggccg tcggtcgggc gagcgatgat gcggaaggtt acctggattt tttcaaaggc 2460 aagataaccg aatcccatct cggcaaggag ctgctggaaa aagtcgagct gacggaggat 2520 aacgccagca gactggagga gttttcgaaa gagtggaagg atgccagtga taagtggaat 2580 gccatgtggg ctgtcaaaat tgagcagacc aaagacggca aacattatgt cgcgggtatt 2640 ggcctcagca tggaggacac ggaggaaggc aaactgagcc agtttctggt tgccgccaat 2700 cgtatcgcat ttattgaccc ggcaaacggg aatgaaacgc cgatgtttgt ggcgcagggc 2760 aaccagatat tcatgaacga cgtgttcctg aagcgcctga cggccccccac cattaccagc 2820 ggcggcaatc ctccggcctt ttccctgaca ccggacggaa agctgaccgc taaaaatgcg 2880 gatatcagcg gtaacgtgaa tgcgaactcc gggacgctca acaacgtcac gattaacgag 2940 aactgtcggg ttctgggaaa attgtccgcg aaccagattg aaggcgatct cgttaaaaca 3000 gtgggcaaag ctttcccccg ggactcccgt gcaccggagc ggtggccatc aggaaccatt 3060 accgtcaggg tttatgacga tcagccgttt gaccggcaga ttgttattcc ggcggtggca 3120 ttcagcggcg ctaaacatga gaaagagcat actgatattt actcctcatg ccgtctgata 3180 gtgcggaaaa acggtgctga aatttataac cgtaccgcgc tggataatac gctgatttac 3240 agtggcgtta ttgatatgcc tgccggtcac ggtcacatga cactggagtt ttcggtgtca 3300 gcatggctgg taaataactg gtatcccaca gcaagtatca gcgatttgct ggttgtggtg 3360 atgaagaaag ccactgcagg catcacgatt agc 3393 <210> 28 <211> 2325 <212> DNA <213> Escherichia phage lambda (Bacteriophage lambda) <400> 28 atggcagtaa agatttcagg agtcctgaaa gacggcacag gaaaaccggt acagaactgc 60 accattcagc tgaaagccag acgtaacagc accacggtgg tggtgaacac ggtgggctca 120 gagaatccgg atgaagccgg gcgttacagc atggatgtgg agtacggtca gtacagtgtc 180 atcctgcagg ttgacggttt tccaccatcg cacgccggga ccatcaccgt gtatgaagat 240 tcacaaccgg ggacgctgaa tgattttctc tgtgccatga cggaggatga tgcccggccg 300 gaggtgctgc gtcgtcttga actgatggtg gaagaggtgg cgcgtaacgc gtccgtggtg 360 gcacagagta cggcagacgc gaagaaatca gccggcgatg ccagtgcatc agctgctcag 420 gtcgcggccc ttgtgactga tgcaactgac tcagcacgcg ccgccagcac gtccgccgga 480 caggctgcat cgtcagctca ggaagcgtcc tccggcgcag aagcggcatc agcaaaggcc 540 actgaagcgg aaaaaagtgc cgcagccgca gagtcctcaa aaaacgcggc ggccaccagt 600 gccggtgcgg cgaaaacgtc agaaacgaat gctgcagcgt cacaacaatc agccgccacg 660 tctgcctcca ccgcggccac gaaagcgtca gaggccgcca cttcagcacg agatgcggtg 720 gcctcaaaag aggcagcaaa atcatcagaa acgaacgcat catcaagtgc cggtcgtgca 780 gcttcctcgg caacggcggc agaaaattct gccagggcgg caaaaacgtc cgagacgaat 840 gccaggtcat ctgaaacagc agcggaacgg agcgcctctg ccgcggcaga cgcaaaaaca 900 gcggcggcgg ggagtgcgtc aacggcatcc acgaaggcga cagaggctgc gggaagtgcg 960 gtatcagcat cgcagagcaa aagtgcggca gaagcggcgg caatacgtgc aaaaaattcg 1020 gcaaaacgtg cagaagatat agcttcagct gtcgcgcttg aggatgcgga cacaacgaga 1080 aaggggatag tgcagctcag cagtgcaacc aacagcacgt ctgaaacgct tgctgcaacg 1140 ccaaaggcgg ttaaggtggt aatggatgag actaatcgta aggcacctct ggacagtccg 1200 gcactgaccg gaacgccaac agcaccaacc gcgctcaggg gaacaaacaa tacccagatt 1260 gcgaacaccg cttttgtact ggccgcgatt gcagatgtta tcgacgcgtc acctgacgca 1320 ctgaatacgc tgaatgaact ggccgcagcg ctcgggaatg atccagattt tgctaccacc 1380 atgactaacg cgcttgcggg taaacaaccg aagaatgcga cactgacggc gctggcaggg 1440 ctttccacgg cgaaaaataa attaccgtat tttgcggaaa atgatgccgc cagcctgact 1500 gaactgactc aggttggcag ggatattctg gcaaaaaatt ccgttgcaga tgttcttgaa 1560 taccttgggg ccggtgagaa ttcggccttt ccggcaggtg cgccgatccc gtggccatca 1620 gatatcgttc cgtctggcta cgtcctgatg caggggcagg cgtttgacaa atcagcctac 1680 ccaaaacttg ctgtcgcgta tccatcgggt gtgcttcctg atatgcgagg ctggacaatc 1740 aaggggaaac ccgccagcgg tcgtgctgta ttgtctcagg aacaggatgg aattaagtcg 1800 cacacccaca gtgccagtgc atccggtacg gatttgggga cgaaaaccac atcgtcgttt 1860 gattacggga cgaaaacaac aggcagtttc gattacggca ccaaatcgac gaataacacg 1920 ggggctcatg ctcacagtct gagcggttca acaggggccg cgggtgctca tgcccacaca 1980 agtggtttaa ggatgaacag ttctggctgg agtcagtatg gaacagcaac cattacagga 2040 agtttatcca cagttaaagg aaccagcaca cagggtattg cttatttatc gaaaacggac 2100 agtcagggca gccacagtca ctcattgtcc ggtacagccg tgagtgccgg tgcacatgcg 2160 catacagttg gtattggtgc gcaccagcat ccggttgtta tcggtgctca tgcccattct 2220 ttcagtattg gttcacacgg acacaccatc accgttaacg ctgcgggtaa cgcggaaaac 2280 accgtcaaaa acatgcatt taactatatt gtgaggcttg cataa 2325 <210> 29 <211> 585 <212> DNA <213> Escherichia phage lambda (Bacteriophage lambda) <400> 29 atggcattca gaatgagtga acaaccacgg accataaaaa tttataatct gctggccgga 60 actaatgaat ttattggtga aggtgacgca tatattccgc ctcataccgg tctgcctgca 120 aacagtaccg atattgcacc gccagatatt ccggctggct ttgtggctgt tttcaacagt 180 gatgaggcat cgtggcatct cgttgaagac catcggggta aaaccgtcta tgacgtggct 240 tccggcgacg cgttatttat ttctgaactc ggtccgttac cggaaaattt tacctggtta 300 tcgccgggag gggaatatca gaagtggaac ggcacagcct gggtgaagga tacggaagca 360 gaaaaactgt tccggatccg ggaggcggaa gaaacaaaaa aaagcctgat gcaggtagcc 420 agtgagcata ttgcgccgct tcaggatgct gcagatctgg aaattgcaac gaaggaagaa 480 acctcgttgc tggaagcctg gaagaagtat cgggtgttgc tgaaccgtgt tgatacatca 540 actgcacctg atattgagtg gcctgctgtc cctgttatgg agtaa 585 <210> 30 <211> 3585 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding STF Lambda-WW11.2 <400> 30 atggcagtaa agatttcagg agtcctgaaa gacggcacag gaaaaccggt acagaactgc 60 accattcagc tgaaagccag acgtaacagc accacggtgg tggtgaacac ggtgggctca 120 gagaatccgg atgaagccgg gcgttacagc atggatgtgg agtacggtca gtacagtgtc 180 atcctgcagg ttgacggttt tccaccatcg cacgccggga ccatcaccgt gtatgaagat 240 tcacaaccgg ggacgctgaa tgattttctc tgtgccatga cggaggatga tgcccggccg 300 gaggtgctgc gtcgtcttga actgatggtg gaagaggtgg cgcgtaacgc gtccgtggtg 360 gcacagagta cggcagacgc gaagaaatca gccggcgatg ccagtgcatc agctgctcag 420 gtcgcggccc ttgtgactga tgcaactgac tcagcacgcg ccgccagcac gtccgccgga 480 caggctgcat cgtcagctca ggaagcgtcc tccggcgcag aagcggcatc agcaaaggcc 540 actgaagcgg aaaaaagtgc cgcagccgca gagtcctcaa aaaacgcggc ggccaccagt 600 gccggtgcgg cgaaaacgtc agaaacgaat gctgcagcgt cacaacaatc agccgccacg 660 tctgcctcca ccgcggccac gaaagcgtca gaggccgcca cttcagcacg agatgcggtg 720 gcctcaaaag aggcagcaaa atcatcagaa acgaacgcat catcaagtgc cggtcgtgca 780 gcttcctcgg caacggcggc agaaaattct gccagggcgg caaaaacgtc cgagacgaat 840 gccaggtcat ctgaaacagc agcggaacgg agcgcctctg ccgcggcaga cgcaaaaaca 900 gcggcggcgg ggagtgcgtc aacggcatcc acgaaggcga cagaggctgc gggaagtgcg 960 gtatcagcat cgcagagcaa aagtgcggca gaagcggcgg caatacgtgc aaaaaattcg 1020 gcaaaacgtg cagaagatat agcttcagct gtcgcgcttg aggatgcgga cacaacgaga 1080 aaggggatag tgcagctcag cagtgcaacc aacagcacgt ctgaaacgct tgctgcaacg 1140 ccaaaggcgg ttaaggtggt aatggatgag actaatcgta aggcacctct ggacagtccg 1200 gcactgaccg gaacgccaac agcaccaacc gcgctcaggg gaacaaacaa tacccagatt 1260 gcgaacaccg cttttgtact ggccgcgatt gcagatgtta tcgacgcgtc acctgacgca 1320 ctgaatacgc tgaatgaact ggccgcagcg ctcgggaatg atccagattt tgctaccacc 1380 atgactaacg cgcttgcggg taaacaaccg aagaatgcga cactgacggc gctggcaggg 1440 ctttccacgg cgaaaaataa attaccgtat tttgcggaaa atgatgccgc cagcctgact 1500 gaactgactc aggttggcag ggatattctg gcaaaaaatt ccgttgcaga tgttcttgaa 1560 taccttgggg ccggtgagaa ttcggctaag caggatggcc tgagtctaat tggtcagtgt 1620 aatagtattg ctgatttaag aaacgtagaa ccagttatgg atggtcaacg cattcttgtt 1680 aaacagcata ctgctggaac aggtaaaggt ggtggcacat tccaggcaaa gctttctggg 1740 gtaggtttaa cagatgataa tggtgtaacc gttaagacgg ttgggggtgc agcatgggtt 1800 cgtgttggag cagaccgagt taacccatac atgtatggtg cacttggttc cggtaatgat 1860 gataccttag cagtacaggc atgtcttaat agtggtaaag ctaccaatat tacagatgtt 1920 caccatgttt ctaacgttac aatgaataag agcagtgcct ctatttatgg ttctggattg 1980 cactatacca gactacatca actacctgct gctgtaggtt ctttgcttac tattgcagat 2040 acctgctcat tgactgttat tgatagtctt gggctgtatg gcaggggtta taagcagggt 2100 actgctttta ccacaggtac tcgcggcatt gatgtactaa ccccaactgg tttatccaat 2160 aactacccag accatactac ttcagaccca cgcagagatt tggttattac taaggttcat 2220 attgcaggtt ttgatgagta tggccttaat gtggactctg gtaactttag cgttactaca 2280 gagtctgtat tgattaacca cattaaacag gttggtgcac gttgtgccac tactgactgg 2340 acttggacaa acatccagat taatacctgc ggtaaacaat gtcttatttt ggatggctgt 2400 ggcaatggtc gtatcatcgg aggtaagttt atctgggcca actggttacc atatggtgca 2460 tccggtcagt ttccgggtat taccatcaat aacagtcaaa acatggttat caacggtatt 2520 gaggtgcagg actgtggtgg taatggtatt gagtttactg atagctactc aatcagcatg 2580 aatggattga atactaaccg taacggtatt aactctacgg cagaattcta taacctggtg 2640 ttcaatcgtt ctgatgtaga aatcaatggt tttgttggtc ttaactataa agttaatagt 2700 ggctcaccag acaattctag ctcaggcaac cttaagttct tgtcgagtga ttgtaatgta 2760 actcttaatg gaaaacttga gacaggctat atgggtatag agttcattgg cgatactcgt 2820 gtaattcagc caacgtcttc ttacattaaa cttaacggtc tggttaacta tgctggttct 2880 ggtattcaga ctattaatga agtacctact tttgatggag ttagtactac tcctgtatac 2940 gtaccaattt cttctgttgt tgggcagacc aatggattac gcctgtctca agctaacaaa 3000 gacaaggtta tttatactcg taaggttggg ccagaaggtg taacaatggc tgctgttatt 3060 actccaacaa ttgggggggc agagatattt aacctcatgg ctattggtac aggttttagc 3120 tctgcaagta acagtctgta tttccagtta gaaatagctg ctgatggttc tcagaatgtg 3180 tctttacttc tgagtgggga tggtactacg cagattctgc ctggtacttt acctgctgca 3240 cttaagttac aatccggcga accgtatcat gtagctttgg gggctaaggc tggatacttc 3300 tggtggagta ttttaaatct caatactggt aagcgtatcc gtcgttcctt ccgaggtgct 3360 tacttaccta aaccattcgc ttctatcttt ggtttagtca gttctccagt attcttctct 3420 ggggcaggta ttggtgattc aggatgctct ggtgttggtg ccaagattta tcttggttcc 3480 ttctctgacg agaatgatta tgtatcctct cgttattata gtttggttaa cccagttgac 3540 ccaactaaga tgtatagctt ccgtatatta gacagtacta tttaa 3585 <210> 31 <211> 2274 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding STF Lambda-75 <400> 31 atggcagtaa agatttcagg agtcctgaaa gacggcacag gaaaaccggt acagaactgc 60 accattcagc tgaaagccag acgtaacagc accacggtgg tggtgaacac ggtgggctca 120 gagaatccgg atgaagccgg gcgttacagc atggatgtgg agtacggtca gtacagtgtc 180 atcctgcagg ttgacggttt tccaccatcg cacgccggga ccatcaccgt gtatgaagat 240 tcacaaccgg ggacgctgaa tgattttctc tgtgccatga cggaggatga tgcccggccg 300 gaggtgctgc gtcgtcttga actgatggtg gaagaggtgg cgcgtaacgc gtccgtggtg 360 gcacagagta cggcagacgc gaagaaatca gccggcgatg ccagtgcatc agctgctcag 420 gtcgcggccc ttgtgactga tgcaactgac tcagcacgcg ccgccagcac gtccgccgga 480 caggctgcat cgtcagctca ggaagcgtcc tccggcgcag aagcggcatc agcaaaggcc 540 actgaagcgg aaaaaagtgc cgcagccgca gagtcctcaa aaaacgcggc ggccaccagt 600 gccggtgcgg cgaaaacgtc agaaacgaat gctgcagcgt cacaacaatc agccgccacg 660 tctgcctcca ccgcggccac gaaagcgtca gaggccgcca cttcagcacg agatgcggtg 720 gcctcaaaag aggcagcaaa atcatcagaa acgaacgcat catcaagtgc cggtcgtgca 780 gcttcctcgg caacggcggc agaaaattct gccagggcgg caaaaacgtc cgagacgaat 840 gccaggtcat ctgaaacagc agcggaacgg agcgcctctg ccgcggcaga cgcaaaaaca 900 gcggcggcgg ggagtgcgtc aacggcatcc acgaaggcga cagaggctgc gggaagtgcg 960 gtatcagcat cgcagagcaa aagtgcggca gaagcggcgg caatacgtgc aaaaaattcg 1020 gcaaaacgtg cagaagatat agcttcagct gtcgcgcttg aggatgcgga cacaacgaga 1080 aaggggatag tgcagctcag cagtgcaacc aacagcacgt ctgaaacgct tgctgcaacg 1140 ccaaaggcgg ttaaggtggt aatggatgag actaatcgta aagcgccctt aaacagtcct 1200 gcgctgaccg gaacgccaac aacgccaact gcgcgacagg gaacgaataa tacccaaatc 1260 gcaagcacgg ctttcgttat ggctgcgatt gccgcccttg tagattcgtc acctgacgca 1320 ctgaatacgc tgaacgagtt agcggcggcg ctgggaaacg acccgaattt tgcgaccacc 1380 atgactaacg cgcttgcggg taagcaaccg aaagatgcca ccctgacggc gctggccggg 1440 cttgctactg cggcagacag gtttccgtat tttacgggga atgatgtcgc cagcctggca 1500 accctgacaa aagtcgggcg ggatattctt gcgaaatcga ccgttgctgc cgttatcgaa 1560 tacctcggtt tacgagaact cggcacaagc ggggagaaaa taccgttact cagtacagcg 1620 aatacctgga ccaatcgaca aacactcagc ggtggccttt ctggggaact gtccggcaat 1680 gccgctactg caacaaagct gaaaacagca cgaaaaattg ctggagttgg ttttgatggt 1740 tctagcgata tttcaattag tgccaaaaat gtcaatgcat ttgcactccg acaaacaggt 1800 aatactgtta atggtgatac atccgttgga tggaattggg atagtggtgc atataacgcc 1860 ctgattggtg gtgcatctgc attaattctt cactttaata taaatgctgg tagctgtcct 1920 gccgtacaat tccgtgtgaa ttataaaaat ggtggcattt cctacaggtc ggctcgtgat 1980 ggttatgggt ttgaattagg ttggtcagat ttctatacca cgacacgaaa accttcagcg 2040 ggagatgttg gtgcatatac gcaggcagaa tgtaactcaa ggtttattac aggtattcgc 2100 cttggcggtc tgtcatctgt tcagacatgg aatggtcccg gctggtctga caggtcaggt 2160 tatgtcgtta cgggttcagt taacggaaac cgtgatgaat taattgatac aacacaggca 2220 aggccaattc agtattgcat taatgggacg tggtataacg cggggagtat ttaa 2274 <210> 32 <211> 534 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding lambda-STF75 accessory protein <400> 32 atgatgcact taaaaaacat tattgctggc aaccctaaaa caaaagagca ataccagcta 60 acaaagcaat ttaacatcaa atggctttat tcagatgatg gaaaaaactg gtatgaggaa 120 caaaagaatt tccagccaga cactttgaaa atggtctatg accataacgg cgttattatt 180 tgtattgaaa aggatgtttc agcaattaat ccggaaggcg caagcgtcgt tgaattacct 240 gatattacag caaatcgccg ggctgatatt tcggggaaat ggatgttcaa agatggcgtg 300 gtgataaagc gaacttatac cgaggaagag cagagacaac aagcggaaaa tgaaaagcaa 360 agcctgttgc aacttgtcag ggataaaacc cagctatggg actcacagct acggctgggc 420 atcatttccg acgagaataa gcagaaatta accgagtgga tgctctttgc gcagaaagtc 480 gaatctacag acacctccag cctgccagta acgtatcccg aacaaccaga atga 534 <210> 33 <211> 2238 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding STF Lambda-EB6 <400> 33 atggcagtaa agatttcagg agtcctgaaa gacggcacag gaaaaccggt acagaactgc 60 accattcagc tgaaagccag acgtaacagc accacggtgg tggtgaacac ggtgggctca 120 gagaatccgg atgaagccgg gcgttacagc atggatgtgg agtacggtca gtacagtgtc 180 atcctgcagg ttgacggttt tccaccatcg cacgccggga ccatcaccgt gtatgaagat 240 tcacaaccgg ggacgctgaa tgattttctc tgtgccatga cggaggatga tgcccggccg 300 gaggtgctgc gtcgtcttga actgatggtg gaagaggtgg cgcgtaacgc gtccgtggtg 360 gcacagagta cggcagacgc gaagaaatca gccggcgatg ccagtgcatc agctgctcag 420 gtcgcggccc ttgtgactga tgcaactgac tcagcacgcg ccgccagcac gtccgccgga 480 caggctgcat cgtcagctca ggaagcgtcc tccggcgcag aagcggcatc agcaaaggcc 540 actgaagcgg aaaaaagtgc cgcagccgca gagtcctcaa aaaacgcggc ggccaccagt 600 gccggtgcgg cgaaaacgtc agaaacgaat gctgcagcgt cacaacaatc agccgccacg 660 tctgcctcca ccgcggccac gaaagcgtca gaggccgcca cttcagcacg agatgcggtg 720 gcctcaaaag aggcagcaaa atcatcagaa acgaacgcat catcaagtgc cggtcgtgca 780 gcttcctcgg caacggcggc agaaaattct gccagggcgg caaaaacgtc cgagacgaat 840 gccaggtcat ctgaaacagc agcggaacgg agcgcctctg ccgcggcaga cgcaaaaaca 900 gcggcggcgg ggagtgcgtc aacggcatcc acgaaggcga cagaggctgc gggaagtgcg 960 gtatcagcat cgcagagcaa aagtgcggca gaagcggcgg caatacgtgc aaaaaattcg 1020 gcaaaacgtg cagaagatat agcttcagct gtcgcgcttg aggatgcgga cacaacgaga 1080 aaggggatag tgcagctcag cagtgcaacc aacagcacgt ctgaaacgct tgctgcaacg 1140 ccaaaggcgg ttaagattgc gatggataat gccaatgccc gtctggcaaa agaccggaac 1200 ggagcagata ttcccaataa gccgctgttt atccaaaacc tcggtttaca ggaaacggta 1260 aacaaggctg gtaacgccgt tcaaaagaca ggcgatacct tgtccggcgg acttactttt 1320 gaaaacgact caatccttgc ctggattcgg aatactgact gggcaaagat tggatttaaa 1380 aatgatgccg acagcgacac tgattcatac atgtggtttg aaacaggcga caacggcaat 1440 gaatatttca aatggagaag ccgccagagc accacaacaa aagacctgat gaatcttaaa 1500 tgggatgctt tgtatgttct tgtcaatgcc attgtaaatg gcgaagtcat atcaaaatca 1560 gcaaacggcc tacgtattgc ttatggtaat tacggattct ttattcgtaa tgatggttca 1620 aatacatact tcatgttgac aaactccggt gacaacatgg ggacttataa cggattaagg 1680 ccattatgga ttaataacgc tactggcgct gtttcgatgg ggcgtggtct taatgtttca 1740 ggggagacac tttcagaccg ttttgctatt aacagcagta atggtatgtg gattcagatg 1800 cgcgataaca acgctatctt tgggaaaaat atagttaaca ctgatagcgc tcaggcgtta 1860 cttcgccaga atcacgccga ccgaaagttc atgataggtg gactggggaa caagcaattt 1920 ggcatctaca tgattaataa ctcaaggaca gccaatggca ccgatggtca ggcgtacat 1980 gataataacg gtaactggct ttgtggtgcg caagttattc ccggcaatta tggcaatttt 2040 gactcacgct atgtgagaga tgtccgactt ggcacacgtg ttgttcaatt gatggcgcgt 2100 ggtggtcgtt atgaaaaagc cggacacgca attaccggat taagaatcat tggtgaagta 2160 gatggcgatg atgaagccat cttcaggcca atacaaaaat acatcaatgg cacatggtat 2220 aacgtcgcac aggtgtaa 2238 <210> 34 <211> 528 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding Lambda-EB6 accessory protein <400> 34 atgcagcatt taaaaaatat taagtctgga aatcctaaaa cgaaagaaca atatcagcta 60 acaaagaatt ttgatgttat ctggttatgg tccgaagacg gtaaaaactg gtatgaagaa 120 gtaaataact ttcaggacga caccataaag attgtatacg acgaaaataa tattattgtt 180 gccataacca aagatgcctc aacgcttaat cccgaaggct ttagcgtcgt tgagattcca 240 gatataacag ccaatcgtcg tgccgatgat tcagggaagt ggatgtttaa ggacggagct 300 gtggttaaac ggatttatac ggcagacgag caacaacaac aggccgaatc acaaaaggcc 360 gcgttacttt ccgaagcaga aaacgttatt cagccactgg aacgcgctgt cagactgaat 420 atggcgacgg atgaggaacg cgcacgactg gagtcatggg aacgctacag tgttctggtc 480 agccgtgtgg atacggcaaa gccagaatgg ccacaaaagc ctgaataa 528 <210> 35 <211> 2274 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding STF Lambda-23 <400> 35 atggcagtaa agatttcagg agtcctgaaa gacggcacag gaaaaccggt acagaactgc 60 accattcagc tgaaagccag acgtaacagc accacggtgg tggtgaacac ggtgggctca 120 gagaatccgg atgaagccgg gcgttacagc atggatgtgg agtacggtca gtacagtgtc 180 atcctgcagg ttgacggttt tccaccatcg cacgccggga ccatcaccgt gtatgaagat 240 tcacaaccgg ggacgctgaa tgattttctc tgtgccatga cggaggatga tgcccggccg 300 gaggtgctgc gtcgtcttga actgatggtg gaagaggtgg cgcgtaacgc gtccgtggtg 360 gcacagagta cggcagacgc gaagaaatca gccggcgatg ccagtgcatc agctgctcag 420 gtcgcggccc ttgtgactga tgcaactgac tcagcacgcg ccgccagcac gtccgccgga 480 caggctgcat cgtcagctca ggaagcgtcc tccggcgcag aagcggcatc agcaaaggcc 540 actgaagcgg aaaaaagtgc cgcagccgca gagtcctcaa aaaacgcggc ggccaccagt 600 gccggtgcgg cgaaaacgtc agaaacgaat gctgcagcgt cacaacaatc agccgccacg 660 tctgcctcca ccgcggccac gaaagcgtca gaggccgcca cttcagcacg agatgcggtg 720 gcctcaaaag aggcagcaaa atcatcagaa acgaacgcat catcaagtgc cggtcgtgca 780 gcttcctcgg caacggcggc agaaaattct gccagggcgg caaaaacgtc cgagacgaat 840 gccaggtcat ctgaaacagc agcggaacgg agcgcctctg ccgcggcaga cgcaaaaaca 900 gcggcggcgg ggagtgcgtc aacggcatcc acgaaggcga cagaggctgc gggaagtgcg 960 gtatcagcat cgcagagcaa aagtgcggca gaagcggcgg caatacgtgc aaaaaattcg 1020 gcaaaacgtg cagaagatat agcttcagct gtcgcgcttg aggatgcgga cacaacgaga 1080 aaggggatag tgcagctcag cagtgcaacc aacagcacgt ctgaaacgct tgctgcaacg 1140 ccaaaggcgg ttaaggtggt aatggatgag actaatcgta aagccccatt aaacagcccg 1200 gcgctgaccg gaacgccaac aacaccaact gcgcgacagg gaacgaataa tacccaaatc 1260 gcaagcacgg ctttcgttat ggctgcgatt gccgcccttg tagattcgtc acctgatgca 1320 ctgaacacgc tgaacgagct ggctgcggcg ttgggcaacg acccgaattt tgcgaccacc 1380 atgactaacg cgcttgcggg taagcaaccg aaagatgcca ccctgacggc gctggccggg 1440 cttgctactg cggcagacag gtttccgtat tttacgggga atgatgtcgc cagcctggca 1500 accctgacaa aagtcgggcg ggatattctt gcgaaatcga ccgttgctgc cgttatcgaa 1560 tacctcggtt tacgagaact cggcacaagc ggggagaaaa taccgttact cagtacagcg 1620 aatacctgga ccaatcgaca aacattcagc ggtggccttt ctgggggact gtccggcaat 1680 gccgctactg caacaaagct gaaaacagca cgaaaaattg ctggagttgg ttttgatggt 1740 tctagcgata tttcaattag tgccaaaaat gtcaatgcat ttgcactccg acaaacaggt 1800 aatactgtta atggtgatac atccgttgga tggaattggg atagtggtgc atataacgcc 1860 ctgattggtg gtgcatctgc attaattctt cactttaata taaatgctgg tagctgtcct 1920 gccgtacaat tccgtgtgaa ttataaaaat ggtggcattt cctacaggtc ggctcgtgat 1980 ggttatgggt ttgaattagg ttggtcagat ttctatacca cgacacgaaa accttcagcg 2040 ggagatgttg gtgcatatac gcgggcagaa tgtaactcaa ggtttattac aggtattcgc 2100 cttggcggtc tgtcatctgt tcagacatgg aatggtcccg gctggtctga caggtcaggt 2160 tatgtcgtta cgggttcagt taacggaaac cgtgatgaat taattgatac aacacaggca 2220 aggccaattc agtattgcat taatgggacg tggtataacg cggggagtat ttaa 2274 <210> 36 <211> 531 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding lambda-STF23 accessory protein <400> 36 atgatgcact taaaaaacat tactgctggc aaccctaaaa caaaagagca ataccagcta 60 acaaagcaat ttaacatcaa atggctttat tcagatgatg gaaaaaactg gtatgaggaa 120 caaaagaatt tccagccaga cactttgaaa atggtctatg accataacgg cgttattatt 180 tgtattgaaa aggatgtttc agcaattaat ccggaaggcg caagcgtcgt tgaattacct 240 gatattacag caaatcgccg ggctgatatt tcggggaaat ggttgttcaa agatggcgta 300 gtgataaagc gaacttatac cgaggaagag cagaggcaac aagcggaaaa tgaaaagcaa 360 agcctgttgc aacttgtcag ggataaaacc cagctatggg actcacagct acggctgggc 420 atcatttccg acgagaataa acaaaaatta accgagtgga tgctctatgc gcagaaagtc 480 gaatctacag acacctccag cctgccagta acgtttcccg aacaaccaga a 531 <210> 37 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Insertion site SAGDAS <400> 37 Ser Ala Gly Asp Ala Ser 1 5 <210> 38 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Insertion site ADAKKS <400> 38 Ala Asp Ala Lys Lys Ser 1 5 <210> 39 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Insertion site MDETNR <400> 39 Met Asp Glu Thr Asn Arg 1 5 <210> 40 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Insertion site SASAAA <400> 40 Ser Ala Ser Ala Ala Ala 1 5 <210> 41 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Insertion site GAGENS <400> 41 Gly Ala Gly Glu Asn Ser 1 5 <210> 42 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Insertion point 1 Figure 7 <400> 42 Tyr Leu Asp Phe Phe Lys 1 5 <210> 43 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Insertion point 2 Figure 7 <400> 43 Asn Ala Asp Ile Ser Gly 1 5 <210> 44 <211> 1186 <212> PRT <213> Artificial Sequence <220> <223> Chimeric STF-V10 <400> 44 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Ala Gly Asp Ala Ser Ala Ser Ala Ala Gln Val Ala Ala Leu 130 135 140 Val Thr Asp Ala Thr Asp Ser Ala Arg Ala Ala Ser Thr Ser Ala Gly 145 150 155 160 Gln Ala Ala Ser Ser Ala Gln Glu Ala Ser Ser Gly Ala Glu Ala Ala 165 170 175 Ser Ala Lys Ala Thr Glu Ala Glu Lys Ser Ala Ala Ala Ala Glu Ser 180 185 190 Ser Lys Asn Ala Ala Ala Thr Ser Ala Gly Ala Ala Lys Thr Ser Glu 195 200 205 Thr Asn Ala Ala Ala Ser Gln Gln Ser Ala Ala Thr Ser Ala Ser Thr 210 215 220 Ala Ala Thr Lys Ala Ser Glu Ala Ala Thr Ser Ala Arg Asp Ala Val 225 230 235 240 Ala Ser Lys Glu Ala Ala Lys Ser Ser Glu Thr Asn Ala Ser Ser Ser 245 250 255 Ala Gly Arg Ala Ala Ser Ser Ala Thr Ala Ala Glu Asn Ser Ala Arg 260 265 270 Ala Ala Lys Thr Ser Glu Thr Asn Ala Arg Ser Ser Glu Thr Ala Ala 275 280 285 Glu Arg Ser Ala Ser Ala Ala Ala Asp Ala Lys Thr Ala Ala Ala Gly 290 295 300 Ser Ala Ser Thr Ala Ser Thr Lys Ala Thr Glu Ala Ala Gly Ser Ala 305 310 315 320 Val Ser Ala Ser Gln Ser Lys Ser Ala Ala Glu Ala Ala Ala Ile Arg 325 330 335 Ala Lys Asn Ser Ala Lys Arg Ala Glu Asp Ile Ala Ser Ala Val Ala 340 345 350 Leu Glu Asp Ala Asp Thr Thr Arg Lys Gly Ile Val Gln Leu Ser Ser 355 360 365 Ala Thr Asn Ser Thr Ser Glu Thr Leu Ala Ala Thr Pro Lys Ala Val 370 375 380 Lys Val Val Met Asp Glu Thr Asn Arg Lys Ala Pro Leu Asp Ser Pro 385 390 395 400 Ala Leu Thr Gly Thr Pro Thr Ala Pro Thr Ala Leu Arg Gly Thr Asn 405 410 415 Asn Thr Gln Ile Ala Asn Thr Ala Phe Val Leu Ala Ala Ile Ala Asp 420 425 430 Val Ile Asp Ala Ser Pro Asp Ala Leu Asn Thr Leu Asn Glu Leu Ala 435 440 445 Ala Ala Leu Gly Asn Asp Pro Asp Phe Ala Thr Thr Met Thr Asn Ala 450 455 460 Leu Ala Gly Lys Gln Pro Lys Asn Ala Thr Leu Thr Ala Leu Ala Gly 465 470 475 480 Leu Ser Thr Ala Lys Asn Lys Leu Pro Tyr Phe Ala Glu Asn Asp Ala 485 490 495 Ala Ser Leu Thr Glu Leu Thr Gln Val Gly Arg Asp Ile Leu Ala Lys 500 505 510 Asn Ser Val Ala Asp Val Leu Glu Tyr Leu Gly Ala Gly Glu Asn Ser 515 520 525 Ala Ala Asn Asp Gly Phe Lys Phe Ile Gly Gln Cys Pro Asp Ile Leu 530 535 540 Thr Leu Arg Thr Ile Glu Pro Glu Lys Asn Gly Gln Arg Ile Thr Leu 545 550 555 560 Arg Gln His Thr Ile Gly Thr Gly Leu Gly Gly Gly Val Phe Arg Ala 565 570 575 Val Leu Asp Gly Thr Gly Tyr Thr Asp Asp Asp Gly Val Val Ile Lys 580 585 590 Thr Ala Gly Gly Ser Val Trp Leu Arg Val Asn Ala Asp Lys Val Asn 595 600 605 Pro Phe Met Phe Gly Ala Thr Gly Val Ala Asp Asp Thr Ala Ala Leu 610 615 620 Gln Lys Met Leu Glu Cys Gly Arg Ala Ala Glu Leu Gly Thr Asn Val 625 630 635 640 Trp Lys Ala Ser Asn Leu Glu Leu Asn Asn Lys Ser Cys Ser Leu Ser 645 650 655 Gly Ser Gly Leu His Val Ser Arg Ile Glu Gln Ile Ser Gly Ala Thr 660 665 670 Gly Ala Leu Leu Thr Ile Thr Gln Asp Cys Ser Leu Ile Tyr Leu Ser 675 680 685 Asp Cys Gly Leu Tyr Gly Asp Gly Ile Thr Ala Gly Thr Ser Gly Val 690 695 700 Thr Met Glu Thr Gly Asn Pro Gly Gly Ala Pro Ser Tyr Pro Phe Asn 705 710 715 720 Thr Ala Pro Asp Val Arg Arg Asp Leu Tyr Ile Ser Asn Val His Ile 725 730 735 Thr Gly Phe Asp Glu Leu Gly Phe Asp Tyr Pro Glu Thr Asn Phe Ser 740 745 750 Val Ser Thr His Gly Leu Phe Ile Arg Asn Ile Lys Lys Thr Gly Ala 755 760 765 Lys Ile Gly Thr Thr Asp Phe Thr Trp Thr Asn Leu Gln Ile Asp Thr 770 775 780 Cys Gly Gln Glu Cys Leu Val Leu Asp Gly Ala Gly Asn Cys Arg Ile 785 790 795 800 Ile Gly Ala Lys Leu Ile Trp Ala Gly Ser Glu Asn Glu Thr Pro Tyr 805 810 815 Ser Gly Leu Arg Ile Ser Asn Ser Gln Asn Val Asn Met Thr Gly Val 820 825 830 Glu Leu Gln Asp Cys Ala Tyr Asp Gly Leu Tyr Ile Lys Asn Ser Thr 835 840 845 Val Ala Ile Ser Gly Leu Asn Thr Asn Arg Asn Ser Ala Ser Ser Asn 850 855 860 Leu Ser Tyr His Asn Met Val Phe Glu Asn Ser Ile Val Thr Val Asp 865 870 875 880 Gly Tyr Val Cys Arg Asn Tyr Ala Ala Thr Ser Leu Tyr Asp Leu Asn 885 890 895 Ser Gln Ala Gly Asn Val Arg Cys Ile Gly Ser Asp Ser Thr Val Leu 900 905 910 Ile Asn Gly Ile Tyr Glu Ser Glu Val Asn Ser Glu Arg Leu Met Gly 915 920 925 Asp Asn Asn Leu Ile Gln Pro Tyr Ser Gly Asp Leu Ile Ile Asn Gly 930 935 940 Leu Lys Asn Tyr Tyr Thr Tyr Thr Gly Ser Val Lys Asn Asn Ile Pro 945 950 955 960 Thr Phe Asp Gly Val Val Thr Thr Ala Thr Tyr Val Ser Ala Pro Ser 965 970 975 Ile Leu Gly Gln Gly Asn Met Leu Lys Leu Thr Gln Ser Asn Lys Asp 980 985 990 Lys Leu Leu Phe Ser Asp Lys Val Ser Arg His Gly Cys Thr Ile Gly 995 1000 1005 Leu Val Leu Ile Pro Ser Phe Thr Gly Ala Thr Thr Met Thr Ala Phe 1010 1015 1020 Thr Leu Gly Ser Gly Tyr Ser Pro Ser Gly Asn Ser Ala Val Met Gln 1025 1030 1035 1040 Phe Ile Val Asn Ser Ser Gly Val Gln Thr Ile Ala Ile Leu Leu Ser 1045 1050 1055 Gly Asp Gly Ile Thr Gln Thr Leu Thr Ser Asp Leu Thr Thr Glu Gln 1060 1065 1070 Ala Leu Ala Ser Gly Gly Val Tyr His Phe Ala Met Gly Phe Ala Pro 1075 1080 1085 Gly Arg Leu Trp Trp Ser Ile Ile Asp Ile Asn Thr Gly Arg Arg Ile 1090 1095 1100 Arg Arg Ala Tyr Arg Gln Pro Asp Leu His Ala Ala Phe Asn Ser Ile 1105 1110 1115 1120 Phe Asn Ser Gly Thr Ser Ser Ile Thr Ala Phe Ser Gly Pro Leu Ala 1125 1130 1135 Gly Asp Ile Ala Cys Glu Gly Ala Gly Ser His Val Tyr Val Gly Gly 1140 1145 1150 Phe Ser Ser Glu Ser Asp Tyr Ala Ala Ser Arg Met Tyr Gly Leu Phe 1155 1160 1165 Thr Pro Val Asp Leu Asp Lys Gln Tyr Ser Phe Arg Thr Leu Asn Gly 1170 1175 1180 Asn Ile 1185 <210> 45 <211> 1186 <212> PRT <213> Artificial Sequence <220> <223> Chimeric STF-V10f <400> 45 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Ala Gly Asp Ala Ser Ala Ser Ala Ala Gln Val Ala Ala Leu 130 135 140 Val Thr Asp Ala Thr Asp Ser Ala Arg Ala Ala Ser Thr Ser Ala Gly 145 150 155 160 Gln Ala Ala Ser Ser Ala Gln Glu Ala Ser Ser Gly Ala Glu Ala Ala 165 170 175 Ser Ala Lys Ala Thr Glu Ala Glu Lys Ser Ala Ala Ala Ala Glu Ser 180 185 190 Ser Lys Asn Ala Ala Ala Thr Ser Ala Gly Ala Ala Lys Thr Ser Glu 195 200 205 Thr Asn Ala Ala Ala Ser Gln Gln Ser Ala Ala Thr Ser Ala Ser Thr 210 215 220 Ala Ala Thr Lys Ala Ser Glu Ala Ala Thr Ser Ala Arg Asp Ala Val 225 230 235 240 Ala Ser Lys Glu Ala Ala Lys Ser Ser Glu Thr Asn Ala Ser Ser Ser 245 250 255 Ala Gly Arg Ala Ala Ser Ser Ala Thr Ala Ala Glu Asn Ser Ala Arg 260 265 270 Ala Ala Lys Thr Ser Glu Thr Asn Ala Arg Ser Ser Glu Thr Ala Ala 275 280 285 Glu Arg Ser Ala Ser Ala Ala Ala Asp Ala Lys Thr Ala Ala Ala Gly 290 295 300 Ser Ala Ser Thr Ala Ser Thr Lys Ala Thr Glu Ala Ala Gly Ser Ala 305 310 315 320 Val Ser Ala Ser Gln Ser Lys Ser Ala Ala Glu Ala Ala Ala Ile Arg 325 330 335 Ala Lys Asn Ser Ala Lys Arg Ala Glu Asp Ile Ala Ser Ala Val Ala 340 345 350 Leu Glu Asp Ala Asp Thr Thr Arg Lys Gly Ile Val Gln Leu Ser Ser 355 360 365 Ala Thr Asn Ser Thr Ser Glu Thr Leu Ala Ala Thr Pro Lys Ala Val 370 375 380 Lys Val Val Met Asp Glu Thr Asn Arg Lys Ala Pro Leu Asp Ser Pro 385 390 395 400 Ala Leu Thr Gly Thr Pro Thr Ala Pro Thr Ala Leu Arg Gly Thr Asn 405 410 415 Asn Thr Gln Ile Ala Asn Thr Ala Phe Val Leu Ala Ala Ile Ala Asp 420 425 430 Val Ile Asp Ala Ser Pro Asp Ala Leu Asn Thr Leu Asn Glu Leu Ala 435 440 445 Ala Ala Leu Gly Asn Asp Pro Asp Phe Ala Thr Thr Met Thr Asn Ala 450 455 460 Leu Ala Gly Lys Gln Pro Lys Asn Ala Thr Leu Thr Ala Leu Ala Gly 465 470 475 480 Leu Ser Thr Ala Lys Asn Lys Leu Pro Tyr Phe Ala Glu Asn Asp Ala 485 490 495 Ala Ser Leu Thr Glu Leu Thr Gln Val Gly Arg Asp Ile Leu Ala Lys 500 505 510 Asn Ser Val Ala Asp Val Leu Glu Tyr Leu Gly Ala Gly Glu Asn Ser 515 520 525 Ala Ala Asn Asp Gly Phe Ala Phe Ile Gly Gln Cys Pro Asp Ile Leu 530 535 540 Thr Leu Arg Thr Ile Glu Pro Glu Lys Asn Gly Gln Arg Ile Thr Leu 545 550 555 560 Arg Gln His Thr Ile Gly Thr Gly Leu Gly Gly Gly Val Phe Arg Ala 565 570 575 Val Leu Asp Gly Thr Gly Tyr Thr Asp Asp Asp Gly Val Val Ile Lys 580 585 590 Thr Ala Gly Gly Ser Val Trp Leu Arg Val Asn Ala Asp Lys Val Asn 595 600 605 Pro Phe Met Phe Gly Ala Thr Gly Val Ala Asp Asp Thr Ala Ala Leu 610 615 620 Gln Lys Met Leu Glu Cys Gly Arg Ala Ala Glu Leu Gly Thr Asn Val 625 630 635 640 Trp Lys Ala Ser Asn Leu Glu Leu Asn Asn Lys Ser Cys Ser Leu Ser 645 650 655 Gly Ser Gly Leu His Val Ser Arg Ile Glu Gln Ile Ser Gly Ala Thr 660 665 670 Gly Ala Leu Leu Thr Ile Thr Gln Asp Cys Ser Leu Ile Tyr Leu Ser 675 680 685 Asp Cys Gly Leu Tyr Gly Asp Gly Ile Thr Ala Gly Thr Ser Gly Val 690 695 700 Thr Met Glu Thr Gly Asn Pro Gly Gly Ala Pro Ser Tyr Pro Phe Asn 705 710 715 720 Thr Ala Pro Asp Val Arg Arg Asp Leu Tyr Ile Ser Asn Val His Ile 725 730 735 Thr Gly Phe Asp Glu Leu Gly Phe Asp Tyr Pro Glu Thr Asn Phe Ser 740 745 750 Val Ser Thr His Gly Leu Phe Ile Arg Asn Ile Lys Lys Thr Gly Ala 755 760 765 Lys Ile Gly Thr Thr Asp Phe Thr Trp Thr Asn Leu Gln Ile Asp Thr 770 775 780 Cys Gly Gln Glu Cys Leu Val Leu Asp Gly Ala Gly Asn Cys Arg Ile 785 790 795 800 Ile Gly Ala Lys Leu Ile Trp Ala Gly Ser Glu Asn Glu Thr Pro Tyr 805 810 815 Ser Gly Leu Arg Ile Ser Asn Ser Gln Asn Val Asn Met Thr Gly Val 820 825 830 Glu Leu Gln Asp Cys Ala Tyr Asp Gly Leu Tyr Ile Lys Asn Ser Thr 835 840 845 Val Ala Ile Ser Gly Leu Asn Thr Asn Arg Asn Ser Ala Ser Ser Asn 850 855 860 Leu Ser Tyr His Asn Met Val Phe Glu Asn Ser Ile Val Thr Val Asp 865 870 875 880 Gly Tyr Val Cys Arg Asn Tyr Ala Ala Thr Ser Leu Tyr Asp Leu Asn 885 890 895 Ser Gln Ala Gly Asn Val Arg Cys Ile Gly Ser Asp Ser Thr Val Leu 900 905 910 Ile Asn Gly Ile Tyr Glu Ser Glu Val Asn Ser Glu Arg Leu Met Gly 915 920 925 Asp Asn Asn Leu Ile Gln Pro Tyr Ser Gly Asp Leu Ile Ile Asn Gly 930 935 940 Leu Lys Asn Tyr Tyr Thr Tyr Thr Gly Ser Val Lys Asn Asn Ile Pro 945 950 955 960 Thr Phe Asp Gly Val Val Thr Thr Ala Thr Tyr Val Ser Ala Pro Ser 965 970 975 Ile Leu Gly Gln Gly Asn Met Leu Lys Leu Thr Gln Ser Asn Lys Asp 980 985 990 Lys Leu Leu Phe Ser Asp Lys Val Ser Arg His Gly Cys Thr Ile Gly 995 1000 1005 Leu Val Leu Ile Pro Ser Phe Thr Gly Ala Thr Thr Met Thr Ala Phe 1010 1015 1020 Thr Leu Gly Ser Gly Tyr Ser Pro Ser Gly Asn Ser Ala Val Met Gln 1025 1030 1035 1040 Phe Ile Val Asn Ser Ser Gly Val Gln Thr Ile Ala Ile Leu Leu Ser 1045 1050 1055 Gly Asp Gly Ile Thr Gln Thr Leu Thr Ser Asp Leu Thr Thr Glu Gln 1060 1065 1070 Ala Leu Ala Ser Gly Gly Val Tyr His Phe Ala Met Gly Phe Ala Pro 1075 1080 1085 Gly Arg Leu Trp Trp Ser Ile Ile Asp Ile Asn Thr Gly Arg Arg Ile 1090 1095 1100 Arg Arg Ala Tyr Arg Gln Pro Asp Leu His Ala Ala Phe Asn Ser Ile 1105 1110 1115 1120 Phe Asn Ser Gly Thr Ser Ser Ile Thr Ala Phe Ser Gly Pro Leu Ala 1125 1130 1135 Gly Asp Ile Ala Cys Glu Gly Ala Gly Ser His Val Tyr Val Gly Gly 1140 1145 1150 Phe Ser Ser Glu Ser Asp Tyr Ala Ala Ser Arg Met Tyr Gly Leu Phe 1155 1160 1165 Thr Pro Val Asp Leu Asp Lys Gln Tyr Ser Phe Arg Thr Leu Asn Gly 1170 1175 1180 Asn Ile 1185 <210> 46 <211> 1186 <212> PRT <213> Artificial Sequence <220> <223> Chimeric STF-V10a <400> 46 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Ala Gly Asp Ala Ser Ala Ser Ala Ala Gln Val Ala Ala Leu 130 135 140 Val Thr Asp Ala Thr Asp Ser Ala Arg Ala Ala Ser Thr Ser Ala Gly 145 150 155 160 Gln Ala Ala Ser Ser Ala Gln Glu Ala Ser Ser Gly Ala Glu Ala Ala 165 170 175 Ser Ala Lys Ala Thr Glu Ala Glu Lys Ser Ala Ala Ala Ala Glu Ser 180 185 190 Ser Lys Asn Ala Ala Ala Thr Ser Ala Gly Ala Ala Lys Thr Ser Glu 195 200 205 Thr Asn Ala Ala Ala Ser Gln Gln Ser Ala Ala Thr Ser Ala Ser Thr 210 215 220 Ala Ala Thr Lys Ala Ser Glu Ala Ala Thr Ser Ala Arg Asp Ala Val 225 230 235 240 Ala Ser Lys Glu Ala Ala Lys Ser Ser Glu Thr Asn Ala Ser Ser Ser 245 250 255 Ala Gly Arg Ala Ala Ser Ser Ala Thr Ala Ala Glu Asn Ser Ala Arg 260 265 270 Ala Ala Lys Thr Ser Glu Thr Asn Ala Arg Ser Ser Glu Thr Ala Ala 275 280 285 Glu Arg Ser Ala Ser Ala Ala Ala Asp Ala Lys Thr Ala Ala Ala Gly 290 295 300 Ser Ala Ser Thr Ala Ser Thr Lys Ala Thr Glu Ala Ala Gly Ser Ala 305 310 315 320 Val Ser Ala Ser Gln Ser Lys Ser Ala Ala Glu Ala Ala Ala Ile Arg 325 330 335 Ala Lys Asn Ser Ala Lys Arg Ala Glu Asp Ile Ala Ser Ala Val Ala 340 345 350 Leu Glu Asp Ala Asp Thr Thr Arg Lys Gly Ile Val Gln Leu Ser Ser 355 360 365 Ala Thr Asn Ser Thr Ser Glu Thr Leu Ala Ala Thr Pro Lys Ala Val 370 375 380 Lys Val Val Met Asp Glu Thr Asn Arg Lys Ala Pro Leu Asp Ser Pro 385 390 395 400 Ala Leu Thr Gly Thr Pro Thr Ala Pro Thr Ala Leu Arg Gly Thr Asn 405 410 415 Asn Thr Gln Ile Ala Asn Thr Ala Phe Val Leu Ala Ala Ile Ala Asp 420 425 430 Val Ile Asp Ala Ser Pro Asp Ala Leu Asn Thr Leu Asn Glu Leu Ala 435 440 445 Ala Ala Leu Gly Asn Asp Pro Asp Phe Ala Thr Thr Met Thr Asn Ala 450 455 460 Leu Ala Gly Lys Gln Pro Lys Asn Ala Thr Leu Thr Ala Leu Ala Gly 465 470 475 480 Leu Ser Thr Ala Lys Asn Lys Leu Pro Tyr Phe Ala Glu Asn Asp Ala 485 490 495 Ala Ser Leu Thr Glu Leu Thr Gln Val Gly Arg Asp Ile Leu Ala Lys 500 505 510 Asn Ser Val Ala Asp Val Leu Glu Tyr Leu Gly Ala Gly Glu Asn Ser 515 520 525 Ala Ala Asn Asp Gly Ala Ala His Ile Gly Gln Cys Pro Asp Ile Leu 530 535 540 Thr Leu Arg Thr Ile Glu Pro Glu Lys Asn Gly Gln Arg Ile Thr Leu 545 550 555 560 Arg Gln His Thr Ile Gly Thr Gly Leu Gly Gly Gly Val Phe Arg Ala 565 570 575 Val Leu Asp Gly Thr Gly Tyr Thr Asp Asp Asp Gly Val Val Ile Lys 580 585 590 Thr Ala Gly Gly Ser Val Trp Leu Arg Val Asn Ala Asp Lys Val Asn 595 600 605 Pro Phe Met Phe Gly Ala Thr Gly Val Ala Asp Asp Thr Ala Ala Leu 610 615 620 Gln Lys Met Leu Glu Cys Gly Arg Ala Ala Glu Leu Gly Thr Asn Val 625 630 635 640 Trp Lys Ala Ser Asn Leu Glu Leu Asn Asn Lys Ser Cys Ser Leu Ser 645 650 655 Gly Ser Gly Leu His Val Ser Arg Ile Glu Gln Ile Ser Gly Ala Thr 660 665 670 Gly Ala Leu Leu Thr Ile Thr Gln Asp Cys Ser Leu Ile Tyr Leu Ser 675 680 685 Asp Cys Gly Leu Tyr Gly Asp Gly Ile Thr Ala Gly Thr Ser Gly Val 690 695 700 Thr Met Glu Thr Gly Asn Pro Gly Gly Ala Pro Ser Tyr Pro Phe Asn 705 710 715 720 Thr Ala Pro Asp Val Arg Arg Asp Leu Tyr Ile Ser Asn Val His Ile 725 730 735 Thr Gly Phe Asp Glu Leu Gly Phe Asp Tyr Pro Glu Thr Asn Phe Ser 740 745 750 Val Ser Thr His Gly Leu Phe Ile Arg Asn Ile Lys Lys Thr Gly Ala 755 760 765 Lys Ile Gly Thr Thr Asp Phe Thr Trp Thr Asn Leu Gln Ile Asp Thr 770 775 780 Cys Gly Gln Glu Cys Leu Val Leu Asp Gly Ala Gly Asn Cys Arg Ile 785 790 795 800 Ile Gly Ala Lys Leu Ile Trp Ala Gly Ser Glu Asn Glu Thr Pro Tyr 805 810 815 Ser Gly Leu Arg Ile Ser Asn Ser Gln Asn Val Asn Met Thr Gly Val 820 825 830 Glu Leu Gln Asp Cys Ala Tyr Asp Gly Leu Tyr Ile Lys Asn Ser Thr 835 840 845 Val Ala Ile Ser Gly Leu Asn Thr Asn Arg Asn Ser Ala Ser Ser Asn 850 855 860 Leu Ser Tyr His Asn Met Val Phe Glu Asn Ser Ile Val Thr Val Asp 865 870 875 880 Gly Tyr Val Cys Arg Asn Tyr Ala Ala Thr Ser Leu Tyr Asp Leu Asn 885 890 895 Ser Gln Ala Gly Asn Val Arg Cys Ile Gly Ser Asp Ser Thr Val Leu 900 905 910 Ile Asn Gly Ile Tyr Glu Ser Glu Val Asn Ser Glu Arg Leu Met Gly 915 920 925 Asp Asn Asn Leu Ile Gln Pro Tyr Ser Gly Asp Leu Ile Ile Asn Gly 930 935 940 Leu Lys Asn Tyr Tyr Thr Tyr Thr Gly Ser Val Lys Asn Asn Ile Pro 945 950 955 960 Thr Phe Asp Gly Val Val Thr Thr Ala Thr Tyr Val Ser Ala Pro Ser 965 970 975 Ile Leu Gly Gln Gly Asn Met Leu Lys Leu Thr Gln Ser Asn Lys Asp 980 985 990 Lys Leu Leu Phe Ser Asp Lys Val Ser Arg His Gly Cys Thr Ile Gly 995 1000 1005 Leu Val Leu Ile Pro Ser Phe Thr Gly Ala Thr Thr Met Thr Ala Phe 1010 1015 1020 Thr Leu Gly Ser Gly Tyr Ser Pro Ser Gly Asn Ser Ala Val Met Gln 1025 1030 1035 1040 Phe Ile Val Asn Ser Ser Gly Val Gln Thr Ile Ala Ile Leu Leu Ser 1045 1050 1055 Gly Asp Gly Ile Thr Gln Thr Leu Thr Ser Asp Leu Thr Thr Glu Gln 1060 1065 1070 Ala Leu Ala Ser Gly Gly Val Tyr His Phe Ala Met Gly Phe Ala Pro 1075 1080 1085 Gly Arg Leu Trp Trp Ser Ile Ile Asp Ile Asn Thr Gly Arg Arg Ile 1090 1095 1100 Arg Arg Ala Tyr Arg Gln Pro Asp Leu His Ala Ala Phe Asn Ser Ile 1105 1110 1115 1120 Phe Asn Ser Gly Thr Ser Ser Ile Thr Ala Phe Ser Gly Pro Leu Ala 1125 1130 1135 Gly Asp Ile Ala Cys Glu Gly Ala Gly Ser His Val Tyr Val Gly Gly 1140 1145 1150 Phe Ser Ser Glu Ser Asp Tyr Ala Ala Ser Arg Met Tyr Gly Leu Phe 1155 1160 1165 Thr Pro Val Asp Leu Asp Lys Gln Tyr Ser Phe Arg Thr Leu Asn Gly 1170 1175 1180 Asn Ile 1185 <210> 47 <211> 11615 <212> DNA <213> Artificial Sequence <220> <223> Example of payload sequence <400> 47 gtttgcaata agggacaagt tacgagtgta gacacgcaga attatccagc ctttagtctt 60 taggaaggca aagctattgt acgcggtagc cgtcgtagca atttaccaac tgtagaatta 120 ttggacacac gtaacaaggg cttacagttg aagtttaata aggtcacacg caaaaccgct 180 aaggaataat cgcaccgtta gcgaaagaat atttcagagc ggttagtaaa ggttgagtaa 240 agtgagattc caaagtgagc ctttataaaa agtaaagagc tataataaaa ccgtcgatcg 300 gaaaacaatc gcctgaaatc tcaagcacgt tgccctttct aacgtcgcta aggtttcgta 360 aacccgtttg attaggaaga agaataagta acccgattag gtttgagatc gcgggttatc 420 ggtttggatt aaaagtggat accagcggag tcaacgccga cgcaaacgta cagtgatcca 480 atcctgttcc acggtcaagc acaatcagct agcaagatct tggaatagag tcgttgcacc 540 gctttgattt acatgctctc cattgcacaa cattccggaa ggactggctt ctctgccatg 600 atcggataat gaaaaacatc agtatgccct gtcatttttc tttgggtgtc ctcaaataat 660 tgccctcacg ttatcgtatg tgacgcgctc atctatgctc gaagtattcc ttgttctccc 720 atcttttaat agaaagtctt taatgaacgt gtcgttacgc agtgtatgaa ctcttgtttt 780 atagggcaga ctttggcgtg gcctaagtgt gttcgataag aaggcaagga caactagctg 840 acgcgctgta atacggatat tatggcacgg ttgatacaaa cgctgatatc ctgatttgct 900 aatgtgccca acactttagt tgagtgccac gttccgacta caagttgctt caagagggga 960 atttggattt ggcaatagcc ccccgtttct acctcaagag gcgacgagta ttaaccgcgc 1020 cagctttcgg cacaagggcc aaagaagatt ccaatttctt attcccgaat aacctccgaa 1080 tccctgcggg aaaatcaccg accgaatagc ctagaagcaa gggggaacag ataggtataa 1140 ttagcttaag agagtaccag ccgtgacaac accgtagtaa ccacaaactt acgctggggc 1200 ttctttggcg gatttttaca gatactaaca aggtgatttg aagtacctta gttgaggatt 1260 taaacgcgct atccggtagt ctacaaattg ggaaataccg ttcaaagagg gctagaatta 1320 cttaaaagcc ttcacaccgc ctgcgctata cgcgcccact ctcccgttta tccgtccaag 1380 cggaagcagg gcgaacttcc gctaagatat tcttacgtgt aacgtagcta agtatcccaa 1440 atagctggcg tacgcgttga acaccgccta gaggatcggg agtcgccgga cgagcgtgtt 1500 attggggact tacgccagcg tagactacaa cgcgcccaga ttaaccctgc acgtattgcc 1560 ttgaataacg tactaatctc tccggctctc gacaatctat cgagcgactc gattatcaac 1620 gggtgtcttg cagttctaat ctcttgcccc cgcccgtaat agcctccaag tgattcaaga 1680 tagtaaaggg caagagctta ttcggcgttg aaggatagcg gactttcggt caaccacaat 1740 tccccactcg acaaaaccag ccgtgcgaag aactctgaaa gtacaagcaa cccaagaggg 1800 ctgagcctaa actcagctaa ttcctaagtg agctaaagac tcgaagtgac agctattaat 1860 aaatagagcg ggaacgtcga acggtcgtga aagtaatagt acaacgggta ttaacttact 1920 gaggatattg cttgaagctg taccgtttta ttgggtgaac gaataagatc cagcaattca 1980 gccaaagaag ctaccaattt ttagtttaag agtgtcacgt ctgacctcgc gggtggatag 2040 ccgaacgtag agcttacgag ccagcggaaa cagtagccgc aggataagta aggggagtaa 2100 gtgatcgaac gaatcagaag tgacaatata cttaggctgg atctcgtccc gtgaatccca 2160 accctcacca actacgagat aagaggtaag ccagaaatcg gcatggtggc gaccaacgac 2220 tgttcccccc ctgtaactaa tcgttccgtc aaaacctgac ttacttcaag gccaattcca 2280 agcgcaaaca ataccgtcct agttcttcgg ttaagtttcc gaagtaggag tgagcctacc 2340 tccgtttgcg tcttgttacc actgacccag ctatttactt tgtattgcct gcaatcgaat 2400 ttctgaactc tcagatagtg gggataacgg gaaagttcct atatttgcga actaacttag 2460 ccgtccacct cgaagctacc tactcacacc caccccgcgc ggggtaaata aggcactaat 2520 cccagcttag agcttgcgta gcacttagcc acaagttaat taacagttgt ctggtagttt 2580 ggcggtatta gcgagatcct agaagcaagg cagagttagt tctaacctaa agccacaaat 2640 aagacaggtt gccaaagccc gccggaaatt aaatcttgct cagttcggta acggagtttc 2700 cctcccgcgt acttaattcc caataagaaa cgcgcccaag tcctatcagg caaaattcag 2760 ccccttcccg tgttagaacg agggtaaaaa tacaagccga ttgaacaagg gttgggggct 2820 tcaaatcgtc gtttacccca ctttacaacg gagggtaagt agttcaccct atagtacgaa 2880 gcagaactat ttcgaggggc gtgcaataat cgaatcttct gcggttgact taacacgcta 2940 gggacgtgcc ctcgattcag tcgcaggtac tcctactcag actgcctcac acccagctag 3000 tcactgagcg ataaaattga cccgccctct aaggtagcga gtacgtccca aagggctccg 3060 gacagggcta tataggagag tttgatctcg ccccgacaac tgcaaccctc aactccctta 3120 gataatattg ttagccgaag ttgcacgacc cgccgtccac ggactgctct tagggtgtgg 3180 ctccttaatc tgacaacgtg caacccctat cgagggcgat tgtttctgcg aaaggtgttg 3240 tcctaatagt cgcgacattt ggcccttgta ggtgtgaaac cacttagctt cgcgccgtag 3300 tcctaaaggc ccacctattg actttgtttc gggtagcact aggaatctta acaatttgaa 3360 tttggacgtg gaacgcgtac accttgatct tcgaataatt ctagggattt ggaagtcctc 3420 tacgttgaca cacctacaat gctccaagta aatatacgaa taacgcgggc ctcgcggagc 3480 cgttccgaat cgtcacgtgt tcgtttactg ttaattggtg gcaaataagc aatatcgtag 3540 tccgtcaggc ccagccctgt tatccacggc gttatttgtc aaattgcgta gaactggatt 3600 gactgcctga caatacctaa ttatcggtac gaagtccccg aatctgtccg gctatttcac 3660 taatactttc caaacgcccc gtatccaaga agaacgaatt tatccacgct cccgtctttg 3720 ggacgaatac cgctacaagt ggacagagga tcggtacggg cctctaataa atccaacact 3780 ctacgccctc ttcaagagct agaagaacag ggtgcagttg gaaagggaat tatttcgtaa 3840 ggcgagccaa taccgtaatt aattcggaag agttaacacg attggaagta ggaatagttt 3900 ctaaccacgg tactaatcc taataacgga acgctgtctg atagattagt gtcagcgctc 3960 actaccaaag aaaaataaaa agacgctgaa aagcgtcttt ttatttttcg gtccagtgta 4020 actcaggcaa aagcacgtaa tattcgtact caccaaacga aactcatccg gcgcatcgcg 4080 cttcttcctc cgtaagcgtc acccccatta cttaaagagt gcatgtgcat attttgttat 4140 caataaaaaa ggccgcgatt tgcggcctta ttgttcgtct tgccggatta gatagctacc 4200 ggtgctttaa tacccggatg cggatcatag ccttcgattt cgaagtcctc aaaacgataa 4260 tcgaagatgc tttccggttt gcgtttgata atcagtttcg ggagcgggcg tggctcacgg 4320 cttaattgta aatgcgtctg atccatgtga tttgagtaca ggtgagtatc cccaccagtc 4380 caaacaaagt caccaacttc cagatcacac tgctgtgcca tcatatgaac taataaggcg 4440 taggaggcaa tgttaaacgg taagcccaga aacacgtcgc aagaacgctg gtacagttgg 4500 cacgataact taccatccgc aacatagaat tgaaagaagg catgacacgg tgctaaagcc 4560 attttgtcta attcccccac gttccatgcg gacacgataa tccggcgaga gtccggatca 4620 tttttcagtt ggttaagaac ggtagtgatc tgatcaatat gccgaccatc cggcgtaggc 4680 catgcacgcc attgcttacc atacactggc cctaagtcac cgttttcatc tgcccactca 4740 tcccagatgg taacgttatt ctcgtgcagg tacgcaatgt tcgtatcgcc ttgcagaaac 4800 cataataact cgtgaataat agaacggagg tggcaacgct tggtagtgac cagcgggaaa 4860 ccgtcttgca ggttgaaacg catctgatga ccaaagatag acagcgtacc agtgccagta 4920 cgatcattct tctgagtgcc ttcgtccagc actttttgca tcagttccag atactgtttc 4980 attttagctt ccttagcttg cgaaatctcg ataactcaaa aaatagtagt gatcttattt 5040 cattatggtg aaagttgtct tacgtgcaac attttcgcaa aaagttggcg ctttatcaac 5100 actgtccgaa tgacaaatgg ttacaattat tgaacaccct tcggggtgtt tttttgtttc 5160 tggtttcccg aggccgaact tttgttgcaa tggctgtcta ccctgtctac ctgagtaaag 5220 aaaaatacat ttaattcagt atattaactt gggtagacag ccttttttta ctgtctacct 5280 tctgtctacc ctctctacct gattttacct gaatcagaca gggaggtaga cacggggtag 5340 acagtggata aaagcactct accccactga aagcagtgcc attactggca tggttgccag 5400 taaggttgat aaggtagaca aggggaggga caactcaaaa ctttttaaac gagggggtaa 5460 aacgcagatc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga 5520 tttcagtgca atttatctct tcaaatgtag caccggcgcg ccgtgaccaa ttattgaagg 5580 ccgctaacgc ggcctttttt tgtttctggt ttcccgaata gagcgacttc tccccaaaaa 5640 gcctcgcttt cagcacctgt cgtttccttt cttttcagag ggtattttaa ataaaaacat 5700 taagttatga cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa 5760 aacccgcgag gtcgccgccc cgtaacctgt cggatcaccg gaaagaacct gtaaagtgat 5820 aatgattatc atctacatat cacaacgtgc gtaaagggta agtatgaagg tcgtgtactc 5880 catcgctacc aaattccaga aaacagacgc tttcgagcgt cttttttcgt tttggtcacg 5940 acgtacggtg gaagattcgt taccaattga cagctagctc agtcctaggt atatacatac 6000 atgcttgttt gtttgtaaac tactgttttc attaaagagg agaaaggaag ccatgtccat 6060 ctatcaggag tttgttaaca agtattccct gtctaaaacc ctgcgttttg aactgatccc 6120 gcagggcaaa actttggaaa acattaaagc gcgtggcctg attctggatg acgaaaaacg 6180 tgcaaaggat tacaagaaag ctaaacagat catcgacaaa tatcaccagt tctttatcga 6240 agaaattctg tcctcggtgt gcatcagtga ggatctgtta cagaattatt ctgatgtata 6300 ctttaaactt aaaaagtccg atgacgataa tctgcaaaaa gatttcaagt cagccaaaga 6360 taccatcaag aaacagatct cagaatatat taaagatagc gaaaagttca aaaacctgtt 6420 taaccaaaac ctcattgatg ctaagaaagg ccaagaatct gacctgatct tatggctgaa 6480 acagagcaaa gataacggca ttgaactgtt caaagctaat agcgacatca ccgatattga 6540 tgaagcgctc gaaatcatca agtctttcaa aggctggacg acgtatttca aaggttttca 6600 tgaaaaccgt aagaatgtat attcgagcaa cgatattccg acctctatta tttatcgtat 6660 cgtggacgac aacctgccga agtttctgga aaacaaagcg aaatatgaat ctctgaaaga 6720 caaagcaccg gaagctatta actatgaaca gatcaagaaa gatctggcgg aagaactgac 6780 cttcgacatc gactataaaa cctccgaagt taaccagcgt gttttctcac tggacgaggt 6840 tttcgaaatc gctaatttca acaattacct gaatcaatct ggcatcacca aattcaacac 6900 cattattggt ggcaaatttg ttaacggcga aaacaccaag cgtaagggca tcaacgaata 6960 cattaacctc tatagccaac aaatcaacga caaaaccctg aaaaagtata aaatgtccgt 7020 tctgtttaaa cagattttat cggacaccga atctaaatcc ttcgtaattg ataaactgga 7080 agatgatagc gacgttgtca ccacgatgca gagcttttat gagcagattg cggcgttcaa 7140 aaccgtcgaa gagaaatcta ttaaagaaac tctgtccctg ctctttgacg acctcaaagc 7200 gcagaaacta gatctgtcta agatttactt taaaaacgac aaatctctga ccgatctcag 7260 tcaacaagtt ttcgatgact atagcgtgat cggcacggca gttttggaat acatcaccca 7320 acaaatcgcg ccgaaaaatc tggacaaccc gtccaagaag gaacaggaac tgattgcaaa 7380 gaaaacagaa aaagctaaat acctgagctt agaaactatc aaactggcac ttgaggaatt 7440 taataaacat cgtgatattg ataaacagtg tcgttttgag gaaattctgg cgaactttgc 7500 ggcaatcccg atgatcttcg acgaaattgc tcaaaacaaa gacaatctgg cgcagatctc 7560 tatcaagtac cagaatcagg gtaagaaaga tctgcttcaa gcatctgcgg aggacgatgt 7620 caaagcaatt aaagacttat tagatcagac gaataactta ttacacaagc tcaaaatctt 7680 ccacatcagc cagagcgagg acaaggcgaa cattctggat aaagatgaac acttctatct 7740 ggtgttcgaa gaatgttact tcgaactggc aaacatcgta cctctctaca ataaaatccg 7800 caactacatc acgcagaagc cttacagtga cgagaaattc aaactgaact tcgaaaacag 7860 cacgctggcg aacggctggg ataagaacaa agagccggac aacaccgcaa tcctgttcat 7920 caaagacgac aaatactatc tgggcgtaat gaacaagaag aacaacaaga tcttcgacga 7980 taaagcgatc aaagaaaaca agggtgaagg ctataagaaa atcgtgtaca agctcctgcc 8040 gggtgcgaac aaaatgttac cgaaagtgtt cttttccgcg aaaagcatca aattctacaa 8100 cccgtctgag gatattctgc gcatccgcaa tcatagcacg cacactaaaa acggtagccc 8160 gcagaaaggg tatgaaaaat tcgaatttaa tatagaggac tgccgtaaat tcatcgactt 8220 ctataaacag agcatttcca aacatccgga atggaaagac ttcggcttcc gtttctctga 8280 cactcagcgc tataatagca tcgacgagtt ctaccgcgaa gtggagaatc agggctataa 8340 actgaccttc gagaacatta gtgagtcgta catcgactcc gttgtgaatc agggtaaact 8400 gtacctgttt cagatctata ataaagactt tagcgcgtac agcaaaggcc gcccgaatct 8460 gcacaccctt tactggaaag cattatttga cgaacgtaac ctgcaagatg tggtgtataa 8520 actgaacggt gaggcggaac ttttctaccg taaacagagt atcccgaaga aaatcacgca 8580 tccggcaaaa gaagctattg ccaacaaaaa caaagacaac ccgaagaaag aaagtgtatt 8640 cgaatatgac ctgatcaaag ataaacgttt caccgaagat aagttctttt tccactgtcc 8700 gattaccatc aacttcaaat ctagcggtgc gaacaagttc aacgatgaaa ttaacttatt 8760 actgaaagag aaagctaatg acgtacacat cttatctatt gatcgcggtg aacgtcattt 8820 agcatactat acactggtag acggtaaagg taatattatt aaacaggata ctttcaatat 8880 tatcggtaat gaccgtatga aaaccaacta tcacgataag ctggcggcga tcgaaaaaga 8940 tcgtgattct gcgcgtaaag attggaagaa aattaacaat atcaaagaaa tgaaagaagg 9000 ctatctgagc caagtggtgc acgagatcgc aaaactggtg attgaatata acgctatcgt 9060 ggttttcgaa gatctgaact ttggttttaa acgtggtcgc ttcaaagtag aaaaacaggt 9120 gtaccaaaaa ctggaaaaaa tgctgattga aaaactgaac tatctggttt ttaaagacaa 9180 cgaatttgac aaaacgggtg gcgtactccg tgcctatcag cttaccgctc cgttcgaaac 9240 gtttaagaaa atgggtaaac aaacggggat tatctattat gtgccagccg gtttcacctc 9300 caagatttgt ccagttacgg gcttcgttaa ccagctttac ccgaaatacg agagcgttag 9360 caaatctcaa gaatttttca gcaaattcga caagatctgc tataatctgg ataaaggcta 9420 tttcgagttc agctttgatt acaaaaactt cggcgataaa gcggctaaag gtaagtggac 9480 tattgctagc tttggtagcc gtctgattaa ctttcgcaac tccgacaaaa accataattg 9540 ggacacgcgt gaagtgtatc cgaccaaaga actggaaaaa ttactgaaag actattccat 9600 cgaatatggt catggggagt gcattaaagc ggcgatttgc ggtgaatccg ataagaaatt 9660 tttcgccaaa ctgaccagcg tgcttaacac cattctccaa atgcgtaatt ctaaaacggg 9720 tacggagctt gactacctga tttctccggt agccgacgtt aacggcaact tcttcgattc 9780 tcgtcaagca ccgaaaaata tgccacaaga cgcggatgcc aacggtgcat accatatcgg 9840 ccttaaaggc ttaatgttat taggccgtat caagaataat caggagggca agaaattaaa 9900 tctggttatc aaaaacgaag aatacttcga gttcgttcag aatcgtaaca attaatgtat 9960 gcttaagcag atcggtaata aagacgaaca ataagacgct gaaaagcgtc ttttttcgtt 10020 ttggtcctgt tccggcgcga tagtgtgaac atgctataga cttctggtgc tacccgactg 10080 acaattaatc atccggctcg tataatgcta gcaatttcta ctgttgtaga tcattccgga 10140 acgttccagc gctgcaattt ctactgttgt agatctgatt tttcacatgt tacctttcaa 10200 tttctactgt tgtagatccg aaaacgtaaa gcttcagctg taatttctac tgttgtagat 10260 atcatatctg gcgttaatgg agtttcgtga cgaacaataa gtcctcccta acggggggca 10320 atttttattg ataacaaaag taacttcgag cttgtctacc tcctagctcg taaattgcac 10380 gctgatagtc tcccaattgc gaaggaccaa aacgaaaaaa caccctttcg ggtgtctttt 10440 ctggaatttg gtacgcagta ctaggtatcg tgtaagtagc gaaggcccgt acgcgagata 10500 aactgctagg caaccgcgac tctacgactg gtgctcgatt taatttcgct gacgtaaaga 10560 aattatcggc agtgcgtcaa ctgccgtatc tttatcttaa ttaggtagtt ggacaagccc 10620 ttgaaagaaa tagcaagagc ctgcctctct attgaagtca cggcgaaagt cgggtagaaa 10680 tcaaagaaag cagaaattaa atcggagtaa tactaagttg ggataactcc gtaactgact 10740 acgcctttct ctagacttta cttgaccaga tacactgtct ttgacacgtt gaaggattag 10800 agcaatcaaa tccaagactg gctaagcacg aagcaactct tgagtgttaa aaagttactt 10860 cctgtattcg ggacgagggt actagaagat tgcagggact ccgacgttaa gtaaattaca 10920 aagtaataag tatcgttcag gatcacgtta ccgcaataag aagcgagaat aatataattt 10980 ccgaagtgct taccccagta gtgactattc ctataaccct tctgagtgtc cggaggcgga 11040 aatttgccac gaaagagaaa gtatttcccc gacaataata aaggggcgct cctcagcttt 11100 tccacttggt tgggtaagct aggcaactct gaaaggagtt tcggcgaagt gaagccgaca 11160 cctttgaatt gttttagggg cgttattcga gggcaatcgg agctaacttc aagactactt 11220 ctttgttgaa tactaaatag tgcaaaggtc gtgtttcctc aaggatactc cgctaacaat 11280 ataggattcc aatcagattc agcactggcg gtacgggtgt tgcggtgagg cgttcgggtt 11340 tacggctcga agctagcacg gtaggaagcc tgacaatcac caagcaaaag ggccgtcgaa 11400 ggcccacaag atacgaaagc tctcgaagcc ttatccttga ccgatccacc tatttaggca 11460 gttacgcaca aaagctaccc aataatccgt gacaggcaca atatcacgga acaaaaccga 11520 aaactctcgt acacggttag gttttcgcta ggaagaataa acctctatct tgattataag 11580 aaggctcccc aagcaccccc aaaaccgaaa tagcg 11615 <210> 48 <211> 1196 <212> PRT <213> Artificial Sequence <220> <223> Chimeric STF-V10h <400> 48 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Ala Gly Asp Ala Ser Ala Ser Ala Ala Gln Val Ala Ala Leu 130 135 140 Val Thr Asp Ala Thr Asp Ser Ala Arg Ala Ala Ser Thr Ser Ala Gly 145 150 155 160 Gln Ala Ala Ser Ser Ala Gln Glu Ala Ser Ser Gly Ala Glu Ala Ala 165 170 175 Ser Ala Lys Ala Thr Glu Ala Glu Lys Ser Ala Ala Ala Ala Glu Ser 180 185 190 Ser Lys Asn Ala Ala Ala Thr Ser Ala Gly Ala Ala Lys Thr Ser Glu 195 200 205 Thr Asn Ala Ala Ala Ser Gln Gln Ser Ala Ala Thr Ser Ala Ser Thr 210 215 220 Ala Ala Thr Lys Ala Ser Glu Ala Ala Thr Ser Ala Arg Asp Ala Val 225 230 235 240 Ala Ser Lys Glu Ala Ala Lys Ser Ser Glu Thr Asn Ala Ser Ser Ser 245 250 255 Ala Gly Arg Ala Ala Ser Ser Ala Thr Ala Ala Glu Asn Ser Ala Arg 260 265 270 Ala Ala Lys Thr Ser Glu Thr Asn Ala Arg Ser Ser Glu Thr Ala Ala 275 280 285 Glu Arg Ser Ala Ser Ala Ala Ala Asp Ala Lys Thr Ala Ala Ala Gly 290 295 300 Ser Ala Ser Thr Ala Ser Thr Lys Ala Thr Glu Ala Ala Gly Ser Ala 305 310 315 320 Val Ser Ala Ser Gln Ser Lys Ser Ala Ala Glu Ala Ala Ala Ile Arg 325 330 335 Ala Lys Asn Ser Ala Lys Arg Ala Glu Asp Ile Ala Ser Ala Val Ala 340 345 350 Leu Glu Asp Ala Asp Thr Thr Arg Lys Gly Ile Val Gln Leu Ser Ser 355 360 365 Ala Thr Asn Ser Thr Ser Glu Thr Leu Ala Ala Thr Pro Lys Ala Val 370 375 380 Lys Val Val Met Asp Glu Thr Asn Arg Lys Ala Pro Leu Asp Ser Pro 385 390 395 400 Ala Leu Thr Gly Thr Pro Thr Ala Pro Thr Ala Leu Arg Gly Thr Asn 405 410 415 Asn Thr Gln Ile Ala Asn Thr Ala Phe Val Leu Ala Ala Ile Ala Asp 420 425 430 Val Ile Asp Ala Ser Pro Asp Ala Leu Asn Thr Leu Asn Glu Leu Ala 435 440 445 Ala Ala Leu Gly Asn Asp Pro Asp Phe Ala Thr Thr Met Thr Asn Ala 450 455 460 Leu Ala Gly Lys Gln Pro Lys Asn Ala Thr Leu Thr Ala Leu Ala Gly 465 470 475 480 Leu Ser Thr Ala Lys Asn Lys Leu Pro Tyr Phe Ala Glu Asn Asp Ala 485 490 495 Ala Ser Leu Thr Glu Leu Thr Gln Val Gly Arg Asp Ile Leu Ala Lys 500 505 510 Asn Ser Val Ala Asp Val Leu Glu Tyr Leu Gly Ala Gly Glu Asn Ser 515 520 525 Gly Ser Ala Thr Asp Val Met Ile Gln Leu Ala Ala Asn Asp Gly Phe 530 535 540 Lys Phe Ile Gly Gln Cys Pro Asp Ile Leu Thr Leu Arg Thr Ile Glu 545 550 555 560 Pro Glu Lys Asn Gly Gln Arg Ile Thr Leu Arg Gln His Thr Ile Gly 565 570 575 Thr Gly Leu Gly Gly Gly Val Phe Arg Ala Val Leu Asp Gly Thr Gly 580 585 590 Tyr Thr Asp Asp Asp Gly Val Val Ile Lys Thr Ala Gly Gly Ser Val 595 600 605 Trp Leu Arg Val Asn Ala Asp Lys Val Asn Pro Phe Met Phe Gly Ala 610 615 620 Thr Gly Val Ala Asp Asp Thr Ala Ala Leu Gln Lys Met Leu Glu Cys 625 630 635 640 Gly Arg Ala Ala Glu Leu Gly Thr Asn Val Trp Lys Ala Ser Asn Leu 645 650 655 Glu Leu Asn Asn Lys Ser Cys Ser Leu Ser Gly Ser Gly Leu His Val 660 665 670 Ser Arg Ile Glu Gln Ile Ser Gly Ala Thr Gly Ala Leu Leu Thr Ile 675 680 685 Thr Gln Asp Cys Ser Leu Ile Tyr Leu Ser Asp Cys Gly Leu Tyr Gly 690 695 700 Asp Gly Ile Thr Ala Gly Thr Ser Gly Val Thr Met Glu Thr Gly Asn 705 710 715 720 Pro Gly Gly Ala Pro Ser Tyr Pro Phe Asn Thr Ala Pro Asp Val Arg 725 730 735 Arg Asp Leu Tyr Ile Ser Asn Val His Ile Thr Gly Phe Asp Glu Leu 740 745 750 Gly Phe Asp Tyr Pro Glu Thr Asn Phe Ser Val Ser Thr His Gly Leu 755 760 765 Phe Ile Arg Asn Ile Lys Lys Thr Gly Ala Lys Ile Gly Thr Thr Asp 770 775 780 Phe Thr Trp Thr Asn Leu Gln Ile Asp Thr Cys Gly Gln Glu Cys Leu 785 790 795 800 Val Leu Asp Gly Ala Gly Asn Cys Arg Ile Ile Gly Ala Lys Leu Ile 805 810 815 Trp Ala Gly Ser Glu Asn Glu Thr Pro Tyr Ser Gly Leu Arg Ile Ser 820 825 830 Asn Ser Gln Asn Val Asn Met Thr Gly Val Glu Leu Gln Asp Cys Ala 835 840 845 Tyr Asp Gly Leu Tyr Ile Lys Asn Ser Thr Val Ala Ile Ser Gly Leu 850 855 860 Asn Thr Asn Arg Asn Ser Ala Ser Ser Asn Leu Ser Tyr His Asn Met 865 870 875 880 Val Phe Glu Asn Ser Ile Val Thr Val Asp Gly Tyr Val Cys Arg Asn 885 890 895 Tyr Ala Ala Thr Ser Leu Tyr Asp Leu Asn Ser Gln Ala Gly Asn Val 900 905 910 Arg Cys Ile Gly Ser Asp Ser Thr Val Leu Ile Asn Gly Ile Tyr Glu 915 920 925 Ser Glu Val Asn Ser Glu Arg Leu Met Gly Asp Asn Asn Leu Ile Gln 930 935 940 Pro Tyr Ser Gly Asp Leu Ile Ile Asn Gly Leu Lys Asn Tyr Tyr Thr 945 950 955 960 Tyr Thr Gly Ser Val Lys Asn Asn Ile Pro Thr Phe Asp Gly Val Val 965 970 975 Thr Thr Ala Thr Tyr Val Ser Ala Pro Ser Ile Leu Gly Gln Gly Asn 980 985 990 Met Leu Lys Leu Thr Gln Ser Asn Lys Asp Lys Leu Leu Phe Ser Asp 995 1000 1005 Lys Val Ser Arg His Gly Cys Thr Ile Gly Leu Val Leu Ile Pro Ser 1010 1015 1020 Phe Thr Gly Ala Thr Thr Met Thr Ala Phe Thr Leu Gly Ser Gly Tyr 1025 1030 1035 1040 Ser Pro Ser Gly Asn Ser Ala Val Met Gln Phe Ile Val Asn Ser Ser 1045 1050 1055 Gly Val Gln Thr Ile Ala Ile Leu Leu Ser Gly Asp Gly Ile Thr Gln 1060 1065 1070 Thr Leu Thr Ser Asp Leu Thr Thr Glu Gln Ala Leu Ala Ser Gly Gly 1075 1080 1085 Val Tyr His Phe Ala Met Gly Phe Ala Pro Gly Arg Leu Trp Trp Ser 1090 1095 1100 Ile Ile Asp Ile Asn Thr Gly Arg Arg Ile Arg Arg Ala Tyr Arg Gln 1105 1110 1115 1120 Pro Asp Leu His Ala Ala Phe Asn Ser Ile Phe Asn Ser Gly Thr Ser 1125 1130 1135 Ser Ile Thr Ala Phe Ser Gly Pro Leu Ala Gly Asp Ile Ala Cys Glu 1140 1145 1150 Gly Ala Gly Ser His Val Tyr Val Gly Gly Phe Ser Ser Glu Ser Asp 1155 1160 1165 Tyr Ala Ala Ser Arg Met Tyr Gly Leu Phe Thr Pro Val Asp Leu Asp 1170 1175 1180 Lys Gln Tyr Ser Phe Arg Thr Leu Asn Gly Asn Ile 1185 1190 1195 <210> 49 <211> 1132 <212> PRT <213> Artificial Sequence <220> <223> A8 <400> 49 Met Gly Lys Gly Ser Ser Lys Gly His Thr Pro Arg Glu Ala Lys Asp 1 5 10 15 Asn Leu Lys Ser Thr Gln Leu Leu Ser Val Ile Asp Ala Ile Ser Glu 20 25 30 Gly Pro Ile Glu Gly Pro Val Asp Gly Leu Lys Ser Val Leu Leu Asn 35 40 45 Ser Thr Pro Val Leu Asp Thr Glu Gly Asn Thr Asn Ile Ser Gly Val 50 55 60 Thr Val Val Phe Arg Ala Gly Glu Gln Glu Gln Thr Pro Pro Glu Gly 65 70 75 80 Phe Glu Ser Ser Gly Ser Glu Thr Val Leu Gly Thr Glu Val Lys Tyr 85 90 95 Asp Thr Pro Ile Thr Arg Thr Ile Thr Ser Ala Asn Ile Asp Arg Leu 100 105 110 Arg Phe Thr Phe Gly Val Gln Ala Leu Val Glu Thr Thr Ser Lys Gly 115 120 125 Asp Arg Asn Pro Ser Glu Val Arg Leu Leu Val Gln Ile Gln Arg Asn 130 135 140 Gly Gly Trp Val Thr Glu Lys Asp Ile Thr Ile Lys Gly Lys Thr Thr 145 150 155 160 Ser Gln Tyr Leu Ala Ser Val Val Met Gly Asn Leu Pro Pro Arg Pro 165 170 175 Phe Asn Ile Arg Met Arg Arg Met Thr Pro Asp Ser Thr Thr Asp Gln 180 185 190 Leu Gln Asn Lys Thr Leu Trp Ser Ser Tyr Thr Glu Ile Ile Asp Val 195 200 205 Lys Gln Cys Tyr Pro Asn Thr Ala Leu Val Gly Val Gln Val Asp Ser 210 215 220 Glu Gln Phe Gly Ser Gln Gln Val Ser Arg Asn Tyr His Leu Arg Gly 225 230 235 240 Arg Ile Leu Gln Val Pro Ser Asn Tyr Asn Pro Gln Thr Arg Gln Tyr 245 250 255 Ser Gly Ile Trp Asp Gly Thr Phe Lys Pro Ala Tyr Ser Asn Asn Met 260 265 270 Ala Trp Cys Leu Trp Asp Met Leu Thr His Pro Arg Tyr Gly Met Gly 275 280 285 Lys Arg Leu Gly Ala Ala Asp Val Asp Lys Trp Ala Leu Tyr Val Ile 290 295 300 Gly Gln Tyr Cys Asp Gln Ser Val Pro Asp Gly Phe Gly Gly Thr Glu 305 310 315 320 Pro Arg Ile Thr Cys Asn Ala Tyr Leu Thr Thr Gln Arg Lys Ala Trp 325 330 335 Asp Val Leu Ser Asp Phe Cys Ser Ala Met Arg Cys Met Pro Val Trp 340 345 350 Asn Gly Gln Thr Leu Thr Phe Val Gln Asp Arg Pro Ser Asp Lys Thr 355 360 365 Trp Thr Tyr Asn Arg Ser Asn Val Val Met Pro Asp Asp Gly Ala Pro 370 375 380 Phe Arg Tyr Ser Phe Ser Ala Leu Lys Asp Arg His Asn Ala Val Glu 385 390 395 400 Val Asn Trp Ile Asp Pro Asn Asn Gly Trp Glu Thr Ala Thr Glu Leu 405 410 415 Val Glu Asp Thr Gln Ala Ile Ala Arg Tyr Gly Arg Asn Val Thr Lys 420 425 430 Met Asp Ala Phe Gly Cys Thr Ser Arg Gly Gln Ala His Arg Ala Gly 435 440 445 Leu Trp Leu Ile Lys Thr Glu Leu Leu Glu Thr Gln Thr Val Asp Phe 450 455 460 Ser Val Gly Ala Glu Gly Leu Arg His Val Pro Gly Asp Val Ile Glu 465 470 475 480 Ile Cys Asp Asp Asp Tyr Ala Gly Ile Ser Thr Gly Gly Arg Val Leu 485 490 495 Ala Val Asn Ser Gln Thr Arg Thr Leu Thr Leu Asp Arg Glu Ile Thr 500 505 510 Leu Pro Ser Ser Gly Thr Ala Leu Ile Ser Leu Val Asp Gly Ser Gly 515 520 525 Asn Pro Val Ser Val Glu Val Gln Ser Val Thr Asp Gly Val Lys Val 530 535 540 Lys Val Ser Arg Val Pro Asp Gly Val Ala Glu Tyr Ser Val Trp Glu 545 550 555 560 Leu Lys Leu Pro Thr Leu Arg Gln Arg Leu Phe Arg Cys Val Ser Ile 565 570 575 Arg Glu Asn Asp Asp Gly Thr Tyr Ala Ile Thr Ala Val Gln His Val 580 585 590 Pro Glu Lys Glu Ala Ile Val Asp Asn Gly Ala His Phe Asp Gly Glu 595 600 605 Gln Ser Gly Thr Val Asn Gly Val Thr Pro Pro Ala Val Gln His Leu 610 615 620 Thr Ala Glu Val Thr Ala Asp Ser Gly Glu Tyr Gln Val Leu Ala Arg 625 630 635 640 Trp Asp Thr Pro Lys Val Val Lys Gly Val Ser Phe Leu Leu Arg Leu 645 650 655 Thr Val Thr Ala Asp Asp Gly Ser Glu Arg Leu Val Ser Thr Ala Arg 660 665 670 Thr Thr Glu Thr Thr Tyr Arg Phe Thr Gln Leu Ala Leu Gly Asn Tyr 675 680 685 Arg Leu Thr Val Arg Ala Val Asn Ala Trp Gly Gln Gln Gly Asp Pro 690 695 700 Ala Ser Val Ser Phe Arg Ile Ala Ala Pro Ala Ala Pro Ser Arg Ile 705 710 715 720 Glu Leu Thr Pro Gly Tyr Phe Gln Ile Thr Ala Thr Pro His Leu Ala 725 730 735 Val Tyr Asp Pro Thr Val Gln Phe Glu Phe Trp Phe Ser Glu Lys Gln 740 745 750 Ile Ala Asp Ile Arg Gln Val Glu Thr Ser Thr Arg Tyr Leu Gly Thr 755 760 765 Ala Leu Tyr Trp Ile Ala Ala Ser Ile Asn Ile Lys Pro Gly His Asp 770 775 780 Tyr Tyr Phe Tyr Ile Arg Ser Val Asn Thr Val Gly Lys Ser Ala Phe 785 790 795 800 Val Glu Ala Val Gly Arg Ala Ser Asp Asp Ala Glu Gly Tyr Leu Asp 805 810 815 Phe Phe Lys Gly Lys Ile Thr Glu Ser His Leu Gly Lys Glu Leu Leu 820 825 830 Glu Lys Val Glu Leu Thr Glu Asp Asn Ala Ser Arg Leu Glu Glu Phe 835 840 845 Ser Lys Glu Trp Lys Asp Ala Ser Asp Lys Trp Asn Ala Met Trp Ala 850 855 860 Val Lys Ile Glu Gln Thr Lys Asp Gly Lys His Tyr Val Ala Gly Ile 865 870 875 880 Gly Leu Ser Met Glu Asp Thr Glu Glu Gly Lys Leu Ser Gln Phe Leu 885 890 895 Val Ala Ala Asn Arg Ile Ala Phe Ile Asp Pro Ala Asn Gly Asn Glu 900 905 910 Thr Pro Met Phe Val Ala Gln Gly Asn Gln Ile Phe Met Asn Asp Val 915 920 925 Phe Leu Lys Arg Leu Thr Ala Pro Thr Ile Thr Ser Gly Gly Asn Pro 930 935 940 Pro Ala Phe Ser Leu Thr Pro Asp Gly Lys Leu Thr Ala Lys Asn Ala 945 950 955 960 Asp Ile Ser Gly Ser Val Asn Ala Asn Ser Gly Thr Leu Asn Asn Val 965 970 975 Thr Ile Asn Glu Asn Cys Gln Ile Lys Gly Lys Leu Ser Ala Asn Gln 980 985 990 Ile Glu Gly Asp Ile Val Lys Thr Val Ser Lys Ser Phe Pro Arg Thr 995 1000 1005 Asn Ser Tyr Ala Ser Gly Thr Ile Thr Val Arg Ile Ser Asp Asp Gln 1010 1015 1020 Lys Phe Asp Arg Gln Val Met Ile Pro Val Leu Phe Arg Gly Gly 1025 1030 1035 1040 Lys His Glu Asn Phe Asn Ser Asn Asn Gln Gln Ser Tyr Trp Tyr Ser 1045 1050 1055 Thr Cys Arg Leu Arg Val Thr Arg Asn Gly Gln Glu Ile Phe Asn Gln 1060 1065 1070 Ser Thr Thr Asp Ala Gin Gly Val Phe Ser Ser Val Ile Asp Met Pro 1075 1080 1085 Ala Gly Gln Gly Thr Leu Thr Leu Thr Phe Thr Val Ser Ser Ser Gly 1090 1095 1100 Ala Asn Asn Trp Thr Pro Thr Thr Ser Ile Ser Asp Leu Leu Val Val 1105 1110 1115 1120 Val Met Lys Lys Ser Thr Ala Gly Ile Ser Ile Ser 1125 1130 <210> 50 <211> 812 <212> PRT <213> Artificial Sequence <220> <223> chimeric STF lambda-P2 <400> 50 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Ala Gly Asp Ala Ser Ala Ser Ala Ala Gln Val Ala Ala Leu 130 135 140 Val Thr Asp Ala Thr Asp Ser Ala Arg Ala Ala Ser Thr Ser Ala Gly 145 150 155 160 Gln Ala Ala Ser Ser Ala Gln Glu Ala Ser Ser Gly Ala Glu Ala Ala 165 170 175 Ser Ala Lys Ala Thr Glu Ala Glu Lys Ser Ala Ala Ala Ala Glu Ser 180 185 190 Ser Lys Asn Ala Ala Ala Thr Ser Ala Gly Ala Ala Lys Thr Ser Glu 195 200 205 Thr Asn Ala Ala Ala Ser Gln Gln Ser Ala Ala Thr Ser Ala Ser Thr 210 215 220 Ala Ala Thr Lys Ala Ser Glu Ala Ala Thr Ser Ala Arg Asp Ala Val 225 230 235 240 Ala Ser Lys Glu Ala Ala Lys Ser Ser Glu Thr Asn Ala Ser Ser Ser 245 250 255 Ala Gly Arg Ala Ala Ser Ser Ala Thr Ala Ala Glu Asn Ser Ala Arg 260 265 270 Ala Ala Lys Thr Ser Glu Thr Asn Ala Arg Ser Ser Glu Thr Ala Ala 275 280 285 Glu Arg Ser Ala Ser Ala Ala Ala Asp Ala Lys Thr Ala Ala Ala Gly 290 295 300 Ser Ala Ser Thr Ala Ser Thr Lys Ala Thr Glu Ala Ala Gly Ser Ala 305 310 315 320 Val Ser Ala Ser Gln Ser Lys Ser Ala Ala Glu Ala Ala Ala Ile Arg 325 330 335 Ala Lys Asn Ser Ala Lys Arg Ala Glu Asp Ile Ala Ser Ala Val Ala 340 345 350 Leu Glu Asp Ala Asp Thr Thr Arg Lys Gly Ile Val Gln Leu Ser Ser 355 360 365 Ala Thr Asn Ser Thr Ser Glu Thr Leu Ala Ala Thr Pro Lys Ala Val 370 375 380 Lys Val Val Met Asp Glu Thr Asn Arg Lys Ala Pro Leu Asn Ser Pro 385 390 395 400 Ala Leu Thr Gly Thr Pro Thr Thr Pro Thr Ala Arg Gln Gly Thr Asn 405 410 415 Asn Thr Gln Ile Ala Asn Thr Ala Phe Val Met Ala Ala Ile Ala Ala 420 425 430 Leu Val Asp Ser Ser Pro Asp Ala Leu Asn Thr Leu Asn Glu Leu Ala 435 440 445 Ala Ala Leu Gly Asn Asp Pro Asn Phe Ala Thr Thr Met Thr Asn Ala 450 455 460 Leu Ala Gly Lys Gln Pro Lys Asp Ala Thr Leu Thr Ala Leu Ala Gly 465 470 475 480 Leu Ala Thr Ala Ala Asp Arg Phe Pro Tyr Phe Thr Gly Asn Asp Val 485 490 495 Ala Ser Leu Ala Thr Leu Thr Lys Val Gly Arg Asp Ile Leu Ala Lys 500 505 510 Ser Thr Val Ala Ala Val Ile Glu Tyr Leu Gly Leu Gln Glu Thr Val 515 520 525 Asn Arg Ala Gly Asn Ala Val Gln Lys Asn Gly Asp Thr Leu Ser Gly 530 535 540 Gly Leu Thr Phe Glu Asn Asp Ser Ile Leu Ala Trp Ile Arg Asn Thr 545 550 555 560 Asp Trp Ala Lys Ile Gly Phe Lys Asn Asp Ala Asp Gly Asp Thr Asp 565 570 575 Ser Tyr Met Trp Phe Glu Thr Gly Asp Asn Gly Asn Glu Tyr Phe Lys 580 585 590 Trp Arg Ser Arg Gln Ser Thr Thr Thr Lys Asp Leu Met Thr Leu Lys 595 600 605 Trp Asp Ala Leu Asn Ile Leu Val Asn Ala Val Ile Asn Gly Cys Phe 610 615 620 Gly Val Gly Thr Thr Asn Ala Leu Gly Gly Ser Ser Ile Val Leu Gly 625 630 635 640 Asp Asn Asp Thr Gly Phe Lys Gln Asn Gly Asp Gly Ile Leu Asp Val 645 650 655 Tyr Ala Asn Ser Gln Arg Val Phe Arg Phe Gln Asn Gly Val Ala Ile 660 665 670 Ala Phe Lys Asn Ile Gln Ala Gly Asp Ser Lys Lys Phe Ser Leu Ser 675 680 685 Ser Ser Asn Thr Ser Thr Lys Asn Ile Thr Phe Asn Leu Trp Gly Ala 690 695 700 Ser Thr Arg Pro Val Val Ala Glu Leu Gly Asp Glu Ala Gly Trp His 705 710 715 720 Phe Tyr Ser Gln Arg Asn Thr Asp Asn Ser Val Ile Phe Ala Val Asn 725 730 735 Gly Gln Met Gln Pro Ser Asn Trp Gly Asn Phe Asp Ser Arg Tyr Val 740 745 750 Lys Asp Val Arg Leu Gly Thr Arg Val Val Gln Leu Met Ala Arg Gly 755 760 765 Gly Arg Tyr Glu Lys Ala Gly His Thr Ile Thr Gly Leu Arg Ile Ile 770 775 780 Gly Glu Val Asp Gly Asp Asp Glu Ala Ile Phe Arg Pro Ile Gln Lys 785 790 795 800 Tyr Ile Asn Gly Thr Trp Tyr Asn Val Ala Gln Val 805 810 <210> 51 <211> 3558 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding chimeric STF-V10 <400> 51 atggcagtaa agatttcagg agtcctgaaa gacggcacag gaaaaccggt acagaactgc 60 accattcagc tgaaagccag acgtaacagc accacggtgg tggtgaacac ggtgggctca 120 gagaatccgg atgaagccgg gcgttacagc atggatgtgg agtacggtca gtacagtgtc 180 atcctgcagg ttgacggttt tccaccatcg cacgccggga ccatcaccgt gtatgaagat 240 tcacaaccgg ggacgctgaa tgattttctc tgtgccatga cggaggatga tgcccggccg 300 gaggtgctgc gtcgtcttga actgatggtg gaagaggtgg cgcgtaacgc gtccgtggtg 360 gcacagagta cggcagacgc gaagaaatca gccggcgatg ccagtgcatc agctgctcag 420 gtcgcggccc ttgtgactga tgcaactgac tcagcacgcg ccgccagcac gtccgccgga 480 caggctgcat cgtcagctca ggaagcgtcc tccggcgcag aagcggcatc agcaaaggcc 540 actgaagcgg aaaaaagtgc cgcagccgca gagtcctcaa aaaacgcggc ggccaccagt 600 gccggtgcgg cgaaaacgtc agaaacgaat gctgcagcgt cacaacaatc agccgccacg 660 tctgcctcca ccgcggccac gaaagcgtca gaggccgcca cttcagcacg agatgcggtg 720 gcctcaaaag aggcagcaaa atcatcagaa acgaacgcat catcaagtgc cggtcgtgca 780 gcttcctcgg caacggcggc agaaaattct gccagggcgg caaaaacgtc cgagacgaat 840 gccaggtcat ctgaaacagc agcggaacgg agcgcctctg ccgcggcaga cgcaaaaaca 900 gcggcggcgg ggagtgcgtc aacggcatcc acgaaggcga cagaggctgc gggaagtgcg 960 gtatcagcat cgcagagcaa aagtgcggca gaagcggcgg caatacgtgc aaaaaattcg 1020 gcaaaacgtg cagaagatat agcttcagct gtcgcgcttg aggatgcgga cacaacgaga 1080 aaggggatag tgcagctcag cagtgcaacc aacagcacgt ctgaaacgct tgctgcaacg 1140 ccaaaggcgg ttaaggtggt aatggatgag actaatcgta aggcacctct ggacagtccg 1200 gcactgaccg gaacgccaac agcaccaacc gcgctcaggg gaacaaacaa tacccagatt 1260 gcgaacaccg cttttgtact ggccgcgatt gcagatgtta tcgacgcgtc acctgacgca 1320 ctgaatacgc tgaatgaact ggccgcagcg ctcgggaatg atccagattt tgctaccacc 1380 atgactaacg cgcttgcggg taaacaaccg aagaatgcga cactgacggc gctggcaggg 1440 ctttccacgg cgaaaaataa attaccgtat tttgcggaaa atgatgccgc cagcctgact 1500 gaactgactc aggttggcag ggatattctg gcaaaaaatt ccgttgcaga tgttcttgaa 1560 taccttgggg ccggtgagaa ttcggcggca aatgatggct tcaaattcat cggtcagtgc 1620 ccagacatct tgaccctgcg tactatcgag ccggaaaaaa acggtcagcg tatcacctta 1680 cgtcaacata cgattggcac tggcttaggc ggtggcgttt tccgtgcagt tctggacggc 1740 actggctata ccgatgacga cggtgtggtg atcaaaaccg ctggggggcag cgtttggctg 1800 cgtgtcaacg ctgacaaagt taacccgttc atgttcggtg caaccggagt agcggacgac 1860 accgccgccc tgcaaaaaat gctggaatgc ggtcgtgcgg cggaactggg gactaacgta 1920 tggaaagcaa gcaatctgga actgaacaac aaatcttgct ctctgtccgg cagtggcctg 1980 cacgtttctc gtattgaaca gatttccggt gcaaccggag cattgttaac catcacccaa 2040 gactgttcgc tgatttacct gtccgattgt ggcctgtacg gcgatggcat caccgcaggc 2100 acgagcggtg ttactatgga aacgggtaat ccgggtggcg ctccgtctta ccctttcaat 2160 accgctccgg acgttcgtcg tgacctgtac atctctaacg tgcacatcac gggcttcgac 2220 gagctgggtt ttgattatcc ggaaaccaat ttctctgttt cgacgcatgg cctcttcatc 2280 cgtaacatca aaaaaacggg tgcaaagatt ggtactacgg acttcacttg gactaacctg 2340 caaattgata cttgcggtca ggaatgtctg gtgctggacg gtgcgggtaa ctgccgtatt 2400 attggtgcaa aactgatttg ggcaggtagc gaaaacgaaa cgccatactc tggcctgcgt 2460 attagcaact ctcaaaatgt aaatatgact ggcgtagagt tacaagactg cgcgtatgat 2520 ggtttataca tcaagaactc tacggttgca atttcaggct taaacaccaa tcgcaatagc 2580 gcatcctcta atctgtccta ccataacatg gtattcgaaa attctattgt aactgttgat 2640 ggttatgtgt gtcgtaacta cgcggcgact tcgctgtacg acctgaacag ccaagcaggc 2700 aacgtccgtt gcatcggtag cgacagcacc gttttaatca acggcatcta cgaaagcgaa 2760 gtcaatagcg agcgcctgat gggtgataac aacctgatcc agccgtatag tggtgatctg 2820 atcattaacg gcctgaaaaa ttactacacc tatactggta gcgtaaaaaa caacattccg 2880 accttcgacg gcgttgttac tacggcaacc tatgtgagcg caccgtctat tctgggtcag 2940 ggcaatatgc tcaaactgac ccagtctaat aaagacaaac tgttatttag cgataaagtt 3000 agccgtcatg gctgtaccat cggcttagtt ctgattccgt cctttacggg cgcgaccact 3060 atgacggcgt tcacgctggg tagcggttac tctccatccg gtaactccgc cgtgatgcag 3120 ttcattgtta acagttccgg tgtacaaacc attgcgattt tattatccgg cgacggtatt 3180 acccaaaccc tgaccagcga tctgaccacg gaacaagcac tggcgagcgg tggcgtgtat 3240 cattttgcaa tgggttttgc gccgggtcgt ttatggtgga gcattatcga tattaacacg 3300 ggcaggcgta ttcgtcgcgc ctaccgtcag ccggatctgc acgcggcgtt caactctatc 3360 ttcaactccg gcacgtcgtc tattaccgca tttagcgggc cactggcggg cgacattgct 3420 tgcgaaggtg caggtagcca tgtatacgtt ggcggttttt cgtcggaatc tgattacgcg 3480 gctagccgta tgtatggcct gttcactccg gtcgatctgg acaagcagta tagcttccgt 3540 accctgaacg gtaacatt 3558 <210> 52 <211> 3558 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding STF-V10f <400> 52 atggcagtaa agatttcagg agtcctgaaa gacggcacag gaaaaccggt acagaactgc 60 accattcagc tgaaagccag acgtaacagc accacggtgg tggtgaacac ggtgggctca 120 gagaatccgg atgaagccgg gcgttacagc atggatgtgg agtacggtca gtacagtgtc 180 atcctgcagg ttgacggttt tccaccatcg cacgccggga ccatcaccgt gtatgaagat 240 tcacaaccgg ggacgctgaa tgattttctc tgtgccatga cggaggatga tgcccggccg 300 gaggtgctgc gtcgtcttga actgatggtg gaagaggtgg cgcgtaacgc gtccgtggtg 360 gcacagagta cggcagacgc gaagaaatca gccggcgatg ccagtgcatc agctgctcag 420 gtcgcggccc ttgtgactga tgcaactgac tcagcacgcg ccgccagcac gtccgccgga 480 caggctgcat cgtcagctca ggaagcgtcc tccggcgcag aagcggcatc agcaaaggcc 540 actgaagcgg aaaaaagtgc cgcagccgca gagtcctcaa aaaacgcggc ggccaccagt 600 gccggtgcgg cgaaaacgtc agaaacgaat gctgcagcgt cacaacaatc agccgccacg 660 tctgcctcca ccgcggccac gaaagcgtca gaggccgcca cttcagcacg agatgcggtg 720 gcctcaaaag aggcagcaaa atcatcagaa acgaacgcat catcaagtgc cggtcgtgca 780 gcttcctcgg caacggcggc agaaaattct gccagggcgg caaaaacgtc cgagacgaat 840 gccaggtcat ctgaaacagc agcggaacgg agcgcctctg ccgcggcaga cgcaaaaaca 900 gcggcggcgg ggagtgcgtc aacggcatcc acgaaggcga cagaggctgc gggaagtgcg 960 gtatcagcat cgcagagcaa aagtgcggca gaagcggcgg caatacgtgc aaaaaattcg 1020 gcaaaacgtg cagaagatat agcttcagct gtcgcgcttg aggatgcgga cacaacgaga 1080 aaggggatag tgcagctcag cagtgcaacc aacagcacgt ctgaaacgct tgctgcaacg 1140 ccaaaggcgg ttaaggtggt aatggatgag actaatcgta aggcacctct ggacagtccg 1200 gcactgaccg gaacgccaac agcaccaacc gcgctcaggg gaacaaacaa tacccagatt 1260 gcgaacaccg cttttgtact ggccgcgatt gcagatgtta tcgacgcgtc acctgacgca 1320 ctgaatacgc tgaatgaact ggccgcagcg ctcgggaatg atccagattt tgctaccacc 1380 atgactaacg cgcttgcggg taaacaaccg aagaatgcga cactgacggc gctggcaggg 1440 ctttccacgg cgaaaaataa attaccgtat tttgcggaaa atgatgccgc cagcctgact 1500 gaactgactc aggttggcag ggatattctg gcaaaaaatt ccgttgcaga tgttcttgaa 1560 taccttgggg ccggtgagaa ttcggcggca aatgatggct tcgcattcat cggtcagtgc 1620 ccagacatct tgaccctgcg tactatcgag ccggaaaaaa acggtcagcg tatcacctta 1680 cgtcaacata cgattggcac tggcttaggc ggtggcgttt tccgtgcagt tctggacggc 1740 actggctata ccgatgacga cggtgtggtg atcaaaaccg ctggggggcag cgtttggctg 1800 cgtgtcaacg ctgacaaagt taacccgttc atgttcggtg caaccggagt agcggacgac 1860 accgccgccc tgcaaaaaat gctggaatgc ggtcgtgcgg cggaactggg gactaacgta 1920 tggaaagcaa gcaatctgga actgaacaac aaatcttgct ctctgtccgg cagtggcctg 1980 cacgtttctc gtattgaaca gatttccggt gcaaccggag cattgttaac catcacccaa 2040 gactgttcgc tgatttacct gtccgattgt ggcctgtacg gcgatggcat caccgcaggc 2100 acgagcggtg ttactatgga aacgggtaat ccgggtggcg ctccgtctta ccctttcaat 2160 accgctccgg acgttcgtcg tgacctgtac atctctaacg tgcacatcac gggcttcgac 2220 gagctgggtt ttgattatcc ggaaaccaat ttctctgttt cgacgcatgg cctcttcatc 2280 cgtaacatca aaaaaacggg tgcaaagatt ggtactacgg acttcacttg gactaacctg 2340 caaattgata cttgcggtca ggaatgtctg gtgctggacg gtgcgggtaa ctgccgtatt 2400 attggtgcaa aactgatttg ggcaggtagc gaaaacgaaa cgccatactc tggcctgcgt 2460 attagcaact ctcaaaatgt aaatatgact ggcgtagagt tacaagactg cgcgtatgat 2520 ggtttataca tcaagaactc tacggttgca atttcaggct taaacaccaa tcgcaatagc 2580 gcatcctcta atctgtccta ccataacatg gtattcgaaa attctattgt aactgttgat 2640 ggttatgtgt gtcgtaacta cgcggcgact tcgctgtacg acctgaacag ccaagcaggc 2700 aacgtccgtt gcatcggtag cgacagcacc gttttaatca acggcatcta cgaaagcgaa 2760 gtcaatagcg agcgcctgat gggtgataac aacctgatcc agccgtatag tggtgatctg 2820 atcattaacg gcctgaaaaa ttactacacc tatactggta gcgtaaaaaa caacattccg 2880 accttcgacg gcgttgttac tacggcaacc tatgtgagcg caccgtctat tctgggtcag 2940 ggcaatatgc tcaaactgac ccagtctaat aaagacaaac tgttatttag cgataaagtt 3000 agccgtcatg gctgtaccat cggcttagtt ctgattccgt cctttacggg cgcgaccact 3060 atgacggcgt tcacgctggg tagcggttac tctccatccg gtaactccgc cgtgatgcag 3120 ttcattgtta acagttccgg tgtacaaacc attgcgattt tattatccgg cgacggtatt 3180 acccaaaccc tgaccagcga tctgaccacg gaacaagcac tggcgagcgg tggcgtgtat 3240 cattttgcaa tgggttttgc gccgggtcgt ttatggtgga gcattatcga tattaacacg 3300 ggcaggcgta ttcgtcgcgc ctaccgtcag ccggatctgc acgcggcgtt caactctatc 3360 ttcaactccg gcacgtcgtc tattaccgca tttagcgggc cactggcggg cgacattgct 3420 tgcgaaggtg caggtagcca tgtatacgtt ggcggttttt cgtcggaatc tgattacgcg 3480 gctagccgta tgtatggcct gttcactccg gtcgatctgg acaagcagta tagcttccgt 3540 accctgaacg gtaacatt 3558 <210> 53 <211> 3558 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding STF-V10a <400> 53 atggcagtaa agatttcagg agtcctgaaa gacggcacag gaaaaccggt acagaactgc 60 accattcagc tgaaagccag acgtaacagc accacggtgg tggtgaacac ggtgggctca 120 gagaatccgg atgaagccgg gcgttacagc atggatgtgg agtacggtca gtacagtgtc 180 atcctgcagg ttgacggttt tccaccatcg cacgccggga ccatcaccgt gtatgaagat 240 tcacaaccgg ggacgctgaa tgattttctc tgtgccatga cggaggatga tgcccggccg 300 gaggtgctgc gtcgtcttga actgatggtg gaagaggtgg cgcgtaacgc gtccgtggtg 360 gcacagagta cggcagacgc gaagaaatca gccggcgatg ccagtgcatc agctgctcag 420 gtcgcggccc ttgtgactga tgcaactgac tcagcacgcg ccgccagcac gtccgccgga 480 caggctgcat cgtcagctca ggaagcgtcc tccggcgcag aagcggcatc agcaaaggcc 540 actgaagcgg aaaaaagtgc cgcagccgca gagtcctcaa aaaacgcggc ggccaccagt 600 gccggtgcgg cgaaaacgtc agaaacgaat gctgcagcgt cacaacaatc agccgccacg 660 tctgcctcca ccgcggccac gaaagcgtca gaggccgcca cttcagcacg agatgcggtg 720 gcctcaaaag aggcagcaaa atcatcagaa acgaacgcat catcaagtgc cggtcgtgca 780 gcttcctcgg caacggcggc agaaaattct gccagggcgg caaaaacgtc cgagacgaat 840 gccaggtcat ctgaaacagc agcggaacgg agcgcctctg ccgcggcaga cgcaaaaaca 900 gcggcggcgg ggagtgcgtc aacggcatcc acgaaggcga cagaggctgc gggaagtgcg 960 gtatcagcat cgcagagcaa aagtgcggca gaagcggcgg caatacgtgc aaaaaattcg 1020 gcaaaacgtg cagaagatat agcttcagct gtcgcgcttg aggatgcgga cacaacgaga 1080 aaggggatag tgcagctcag cagtgcaacc aacagcacgt ctgaaacgct tgctgcaacg 1140 ccaaaggcgg ttaaggtggt aatggatgag actaatcgta aggcacctct ggacagtccg 1200 gcactgaccg gaacgccaac agcaccaacc gcgctcaggg gaacaaacaa tacccagatt 1260 gcgaacaccg cttttgtact ggccgcgatt gcagatgtta tcgacgcgtc acctgacgca 1320 ctgaatacgc tgaatgaact ggccgcagcg ctcgggaatg atccagattt tgctaccacc 1380 atgactaacg cgcttgcggg taaacaaccg aagaatgcga cactgacggc gctggcaggg 1440 ctttccacgg cgaaaaataa attaccgtat tttgcggaaa atgatgccgc cagcctgact 1500 gaactgactc aggttggcag ggatattctg gcaaaaaatt ccgttgcaga tgttcttgaa 1560 taccttgggg ccggtgagaa ttcggcggca aatgatggcg cggcacacat cggtcagtgc 1620 ccagacatct tgaccctgcg tactatcgag ccggaaaaaa acggtcagcg tatcacctta 1680 cgtcaacata cgattggcac tggcttaggc ggtggcgttt tccgtgcagt tctggacggc 1740 actggctata ccgatgacga cggtgtggtg atcaaaaccg ctggggggcag cgtttggctg 1800 cgtgtcaacg ctgacaaagt taacccgttc atgttcggtg caaccggagt agcggacgac 1860 accgccgccc tgcaaaaaat gctggaatgc ggtcgtgcgg cggaactggg gactaacgta 1920 tggaaagcaa gcaatctgga actgaacaac aaatcttgct ctctgtccgg cagtggcctg 1980 cacgtttctc gtattgaaca gatttccggt gcaaccggag cattgttaac catcacccaa 2040 gactgttcgc tgatttacct gtccgattgt ggcctgtacg gcgatggcat caccgcaggc 2100 acgagcggtg ttactatgga aacgggtaat ccgggtggcg ctccgtctta ccctttcaat 2160 accgctccgg acgttcgtcg tgacctgtac atctctaacg tgcacatcac gggcttcgac 2220 gagctgggtt ttgattatcc ggaaaccaat ttctctgttt cgacgcatgg cctcttcatc 2280 cgtaacatca aaaaaacggg tgcaaagatt ggtactacgg acttcacttg gactaacctg 2340 caaattgata cttgcggtca ggaatgtctg gtgctggacg gtgcgggtaa ctgccgtatt 2400 attggtgcaa aactgatttg ggcaggtagc gaaaacgaaa cgccatactc tggcctgcgt 2460 attagcaact ctcaaaatgt aaatatgact ggcgtagagt tacaagactg cgcgtatgat 2520 ggtttataca tcaagaactc tacggttgca atttcaggct taaacaccaa tcgcaatagc 2580 gcatcctcta atctgtccta ccataacatg gtattcgaaa attctattgt aactgttgat 2640 ggttatgtgt gtcgtaacta cgcggcgact tcgctgtacg acctgaacag ccaagcaggc 2700 aacgtccgtt gcatcggtag cgacagcacc gttttaatca acggcatcta cgaaagcgaa 2760 gtcaatagcg agcgcctgat gggtgataac aacctgatcc agccgtatag tggtgatctg 2820 atcattaacg gcctgaaaaa ttactacacc tatactggta gcgtaaaaaa caacattccg 2880 accttcgacg gcgttgttac tacggcaacc tatgtgagcg caccgtctat tctgggtcag 2940 ggcaatatgc tcaaactgac ccagtctaat aaagacaaac tgttatttag cgataaagtt 3000 agccgtcatg gctgtaccat cggcttagtt ctgattccgt cctttacggg cgcgaccact 3060 atgacggcgt tcacgctggg tagcggttac tctccatccg gtaactccgc cgtgatgcag 3120 ttcattgtta acagttccgg tgtacaaacc attgcgattt tattatccgg cgacggtatt 3180 acccaaaccc tgaccagcga tctgaccacg gaacaagcac tggcgagcgg tggcgtgtat 3240 cattttgcaa tgggttttgc gccgggtcgt ttatggtgga gcattatcga tattaacacg 3300 ggcaggcgta ttcgtcgcgc ctaccgtcag ccggatctgc acgcggcgtt caactctatc 3360 ttcaactccg gcacgtcgtc tattaccgca tttagcgggc cactggcggg cgacattgct 3420 tgcgaaggtg caggtagcca tgtatacgtt ggcggttttt cgtcggaatc tgattacgcg 3480 gctagccgta tgtatggcct gttcactccg gtcgatctgg acaagcagta tagcttccgt 3540 accctgaacg gtaacatt 3558 <210> 54 <211> 3588 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding STF-V10h <400> 54 atggcagtaa agatttcagg agtcctgaaa gacggcacag gaaaaccggt acagaactgc 60 accattcagc tgaaagccag acgtaacagc accacggtgg tggtgaacac ggtgggctca 120 gagaatccgg atgaagccgg gcgttacagc atggatgtgg agtacggtca gtacagtgtc 180 atcctgcagg ttgacggttt tccaccatcg cacgccggga ccatcaccgt gtatgaagat 240 tcacaaccgg ggacgctgaa tgattttctc tgtgccatga cggaggatga tgcccggccg 300 gaggtgctgc gtcgtcttga actgatggtg gaagaggtgg cgcgtaacgc gtccgtggtg 360 gcacagagta cggcagacgc gaagaaatca gccggcgatg ccagtgcatc agctgctcag 420 gtcgcggccc ttgtgactga tgcaactgac tcagcacgcg ccgccagcac gtccgccgga 480 caggctgcat cgtcagctca ggaagcgtcc tccggcgcag aagcggcatc agcaaaggcc 540 actgaagcgg aaaaaagtgc cgcagccgca gagtcctcaa aaaacgcggc ggccaccagt 600 gccggtgcgg cgaaaacgtc agaaacgaat gctgcagcgt cacaacaatc agccgccacg 660 tctgcctcca ccgcggccac gaaagcgtca gaggccgcca cttcagcacg agatgcggtg 720 gcctcaaaag aggcagcaaa atcatcagaa acgaacgcat catcaagtgc cggtcgtgca 780 gcttcctcgg caacggcggc agaaaattct gccagggcgg caaaaacgtc cgagacgaat 840 gccaggtcat ctgaaacagc agcggaacgg agcgcctctg ccgcggcaga cgcaaaaaca 900 gcggcggcgg ggagtgcgtc aacggcatcc acgaaggcga cagaggctgc gggaagtgcg 960 gtatcagcat cgcagagcaa aagtgcggca gaagcggcgg caatacgtgc aaaaaattcg 1020 gcaaaacgtg cagaagatat agcttcagct gtcgcgcttg aggatgcgga cacaacgaga 1080 aaggggatag tgcagctcag cagtgcaacc aacagcacgt ctgaaacgct tgctgcaacg 1140 ccaaaggcgg ttaaggtggt aatggatgag actaatcgta aggcacctct ggacagtccg 1200 gcactgaccg gaacgccaac agcaccaacc gcgctcaggg gaacaaacaa tacccagatt 1260 gcgaacaccg cttttgtact ggccgcgatt gcagatgtta tcgacgcgtc acctgacgca 1320 ctgaatacgc tgaatgaact ggccgcagcg ctcgggaatg atccagattt tgctaccacc 1380 atgactaacg cgcttgcggg taaacaaccg aagaatgcga cactgacggc gctggcaggg 1440 ctttccacgg cgaaaaataa attaccgtat tttgcggaaa atgatgccgc cagcctgact 1500 gaactgactc aggttggcag ggatattctg gcaaaaaatt ccgttgcaga tgttcttgaa 1560 taccttgggg ccggtgagaa ttcggggagc gctacagacg ttatgattca gctggcggca 1620 aatgatggct tcaaattcat cggtcagtgc ccagacatct tgaccctgcg tactatcgag 1680 ccggaaaaaa acggtcagcg tatcacctta cgtcaacata cgattggcac tggcttaggc 1740 ggtggcgttt tccgtgcagt tctggacggc actggctata ccgatgacga cggtgtggtg 1800 atcaaaaccg ctgggggcag cgtttggctg cgtgtcaacg ctgacaaagt taacccgttc 1860 atgttcggtg caaccggagt agcggacgac accgccgccc tgcaaaaaat gctggaatgc 1920 ggtcgtgcgg cggaactggg gactaacgta tggaaagcaa gcaatctgga actgaacaac 1980 aaatcttgct ctctgtccgg cagtggcctg cacgtttctc gtattgaaca gatttccggt 2040 gcaaccggag cattgttaac catcacccaa gactgttcgc tgatttacct gtccgattgt 2100 ggcctgtacg gcgatggcat caccgcaggc acgagcggtg ttactatgga aacgggtaat 2160 ccgggtggcg ctccgtctta ccctttcaat accgctccgg acgttcgtcg tgacctgtac 2220 atctctaacg tgcacatcac gggcttcgac gagctgggtt ttgattatcc ggaaaccaat 2280 ttctctgttt cgacgcatgg cctcttcatc cgtaacatca aaaaaacggg tgcaaagatt 2340 ggtactacgg acttcacttg gactaacctg caaattgata cttgcggtca ggaatgtctg 2400 gtgctggacg gtgcgggtaa ctgccgtatt attggtgcaa aactgatttg ggcaggtagc 2460 gaaaacgaaa cgccatactc tggcctgcgt attagcaact ctcaaaatgt aaatatgact 2520 ggcgtagagt tacaagactg cgcgtatgat ggtttataca tcaagaactc tacggttgca 2580 atttcaggct taaacaccaa tcgcaatagc gcatcctcta atctgtccta ccataacatg 2640 gtattcgaaa attctattgt aactgttgat ggttatgtgt gtcgtaacta cgcggcgact 2700 tcgctgtacg acctgaacag ccaagcaggc aacgtccgtt gcatcggtag cgacagcacc 2760 gttttaatca acggcatcta cgaaagcgaa gtcaatagcg agcgcctgat gggtgataac 2820 aacctgatcc agccgtatag tggtgatctg atcattaacg gcctgaaaaa ttactacacc 2880 tatactggta gcgtaaaaaa caacattccg accttcgacg gcgttgttac tacggcaacc 2940 tatgtgagcg caccgtctat tctgggtcag ggcaatatgc tcaaactgac ccagtctaat 3000 aaagacaaac tgttatttag cgataaagtt agccgtcatg gctgtaccat cggcttagtt 3060 ctgattccgt cctttacggg cgcgaccact atgacggcgt tcacgctggg tagcggttac 3120 tctccatccg gtaactccgc cgtgatgcag ttcattgtta acagttccgg tgtacaaacc 3180 attgcgattt tattatccgg cgacggtatt acccaaaccc tgaccagcga tctgaccacg 3240 gaacaagcac tggcgagcgg tggcgtgtat cattttgcaa tgggttttgc gccgggtcgt 3300 ttatggtgga gcattatcga tattaacacg ggcaggcgta ttcgtcgcgc ctaccgtcag 3360 ccggatctgc acgcggcgtt caactctatc ttcaactccg gcacgtcgtc tattaccgca 3420 tttagcgggc cactggcggg cgacattgct tgcgaaggtg caggtagcca tgtatacgtt 3480 ggcggttttt cgtcggaatc tgattacgcg gctagccgta tgtatggcct gttcactccg 3540 gtcgatctgg acaagcagta tagcttccgt accctgaacg gtaacatt 3588 <210> 55 <211> 2032 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding A8 <400> 55 atgggtaaag gaagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaagtcc 60 acgcagttgc tgagtgtgat cgatgccatc agcgaagggc cgattgaagg tccggtggat 120 ggcttaaaaa gcgtgctgct gaacagtacg ccggtgctgg acactgaggg gaataccaac 180 atatccggtg tcacggtggt gttccgggct ggtgagcagg agcagactcc gccggaggga 240 tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga cacgccgatc 300 acccgcacca ttacgtctgc aaacatcgac cgtctgcgct ttaccttcgg tgtacaggca 360 ctggtggaaa ccacctcaaa gggtgacagg aatccgtcgg aagtccgcct gctggttcag 420 atacaacgta acggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccacc 480 tcgcagtatc tggcctcggt ggtgatgggt aacctgccgc cgcgcccgtt taatatccgg 540 atgcgcagga tgacgccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg 600 tcatacactg aaatcatcga tgtgaaacag tgctacccga acacggcact ggtcggcgtg 660 caggtggact cggagcagtt cggcagccag caggtgagcc gtaattatca tctgcgcggg 720 cgtattctgc aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg 780 gacggaacgt ttaaaccggc atacagcaac aacatggcct ggtgtctgtg ggatatgctg 840 acccatccgc gctacggcat ggggaaacgt cttggtgcgg cggatgtgga taaatgggcg 900 ctgtatgtca tcggccagta ctgcgaccag tcagtgccgg acggctttgg cggcacggag 960 ccgcgcatca cctgtaatgc gtacctgacc acacagcgta aggcgtggga tgtgctcagc 1020 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg 1080 caggaccgac cgtcggataa gacgtggacc tataaccgca gtaatgtggt gatgccggat 1140 gatggcgcgc cgttccgcta cagcttcagc gccctgaagg accgccataa tgccgttgag 1200 gtgaactgga ttgacccgaa caacggctgg gagacggcga cagagcttgt tgaagatacg 1260 caggccattg cccgttacgg tcgtaatgtt acgaagatgg atgcctttgg ctgtaccagc 1320 cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cagaactgct ggaaacgcag 1380 accgtggatt tcagcgtcgg cgcagaaggg cttcgccatg taccgggcga tgttattgaa 1440 atctgcgatg atgactatgc cggtatcagc accggtggtc gtgtgctggc ggtgaacagc 1500 cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg taccgcgctg 1560 ataagcctgg ttgacggaag tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac 1620 ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag cgtatgggag 1680 ctgaagctgc cgacgctgcg ccagcgactg ttccgctgcg tgagtatccg tgagaacgac 1740 gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaggc catcgtggat 1800 aacggggcgc actttgacgg cgaacagagt ggcacggtga atggtgtcac gccgccagcg 1860 gtgcagcacc tgaccgcaga agtcactgca gacagcgggg aatatcaggt gctggcgcga 1920 tgggacacac cgaaggtggt gaagggcgtg agtttcctgc tccgtctgac cgtaacagcg 1980 gacgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac at 2032 <210> 56 <211> 2045 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding STF lambda-P2 <400> 56 atggcagtaa agatttcagg agtcctgaaa gacggcacag gaaaaccggt acagaactgc 60 accattcagc tgaaagccag acgtaacagc accacggtgg tggtgaacac ggtgggctca 120 gagaatccgg atgaagccgg gcgttacagc atggatgtgg agtacggtca gtacagtgtc 180 atcctgcagg ttgacggttt tccaccatcg cacgccggga ccatcaccgt gtatgaagat 240 tcacaaccgg ggacgctgaa tgattttctc tgtgccatga cggaggatga tgcccggccg 300 gaggtgctgc gtcgtcttga actgatggtg gaagaggtgg cgcgtaacgc gtccgtggtg 360 gcacagagta cggcagacgc gaagaaatca gccggcgatg ccagtgcatc agctgctcag 420 gtcgcggccc ttgtgactga tgcaactgac tcagcacgcg ccgccagcac gtccgccgga 480 caggctgcat cgtcagctca ggaagcgtcc tccggcgcag aagcggcatc agcaaaggcc 540 actgaagcgg aaaaaagtgc cgcagccgca gagtcctcaa aaaacgcggc ggccaccagt 600 gccggtgcgg cgaaaacgtc agaaacgaat gctgcagcgt cacaacaatc agccgccacg 660 tctgcctcca ccgcggccac gaaagcgtca gaggccgcca cttcagcacg agatgcggtg 720 gcctcaaaag aggcagcaaa atcatcagaa acgaacgcat catcaagtgc cggtcgtgca 780 gcttcctcgg caacggcggc agaaaattct gccagggcgg caaaaacgtc cgagacgaat 840 gccaggtcat ctgaaacagc agcggaacgg agcgcctctg ccgcggcaga cgcaaaaaca 900 gcggcggcgg ggagtgcgtc aacggcatcc acgaaggcga cagaggctgc gggaagtgcg 960 gtatcagcat cgcagagcaa aagtgcggca gaagcggcgg caatacgtgc aaaaaattcg 1020 gcaaaacgtg cagaagatat agcttcagct gtcgcgcttg aggatgcgga cacaacgaga 1080 aaggggatag tgcagctcag cagtgcaacc aacagcacgt ctgaaacgct tgctgcaacg 1140 ccaaaggcgg ttaaggtggt aatggatgag actaatcgta aagcgccatt aaacagccct 1200 gcactgaccg gaacgccaac gacgccaact gcgcgacagg gaacgaataa tactcagatc 1260 gcaaacacgg ctttcgttat ggccgcgatt gccgcccttg tagactcgtc gcctgacgca 1320 ctgaatacgc tgaacgagct ggcggcggcg ctgggcaatg acccgaattt tgctaccacc 1380 atgactaatg cgcttgcggg taagcaaccg aaagatgcta ccctgacggc gctggcgggg 1440 cttgctactg cggcagacag gtttccgtat tttacgggga atgatgttgc cagcctggcg 1500 accctgacaa aagtcgggcg ggatattctg gctaaatcga ccgttgccgc cgttatcgaa 1560 tatctcggtt tacaggaaac ggtaaaccga gccgggaacg ccgtgcaaaa aaatggcgat 1620 accttgtccg gtggacttac ttttgaaaac gactcaatcc ttgcctggat tcgaaatact 1680 gactgggcga agattggatt taaaaatgat gccgatggtg acactgattc atacatgtgg 1740 tttgaaacgg gggataacgg caatgaatat ttcaaatgga gaagccgcca gagtaccaca 1800 acaaaagacc tgatgacgtt gaaatgggat gcactaaata ttcttgttaa tgccgtcatt 1860 aatggctgtt ttggagttgg tacgacgaat gcactaggtg gtagctctat tgttcttggt 1920 gataatgata ccggatttaa acagaatgga gacggtattc ttgatgttta tgctaacagt 1980 cagcgtgtat tccgttttca gaatggagtg gctattgctt ttaaaaatat tcaggcaggt 2040 gatag 2045 <210> 57 <211> 175 <212> PRT <213> Artificial Sequence <220> <223> P2 accessory protein 1 <400> 57 Met Gln His Leu Lys Asn Ile Lys Ser Gly Asn Pro Lys Thr Lys Glu 1 5 10 15 Gln Tyr Gln Leu Thr Lys Asn Phe Asp Val Ile Trp Leu Trp Ser Glu 20 25 30 Asp Gly Lys Asn Trp Tyr Glu Glu Val Lys Asn Phe Gln Pro Asp Thr 35 40 45 Ile Lys Ile Val Tyr Asp Glu Asn Asn Ile Ile Val Ala Ile Thr Arg 50 55 60 Asp Ala Ser Thr Leu Asn Pro Glu Gly Phe Ser Val Val Glu Val Pro 65 70 75 80 Asp Ile Thr Ser Asn Arg Arg Ala Asp Asp Ser Gly Lys Trp Met Phe 85 90 95 Lys Asp Gly Ala Val Val Lys Arg Ile Tyr Thr Ala Asp Glu Gln Gln 100 105 110 Gln Gln Ala Glu Ser Gln Lys Ala Ala Leu Leu Ser Glu Ala Glu Asn 115 120 125 Val Ile Gln Pro Leu Glu Arg Ala Val Arg Leu Asn Met Ala Thr Asp 130 135 140 Glu Glu Arg Ala Arg Leu Glu Ser Trp Glu Arg Tyr Ser Val Leu Val 145 150 155 160 Ser Arg Val Asp Pro Ala Asn Pro Glu Trp Pro Glu Met Pro Gln 165 170 175 <210> 58 <211> 525 <212> DNA <213> Artificial Sequence <220> <223> nucleic acid encoding P2 accessory protein 1 <400> 58 atgcagcatt taaagaacat taagtcaggt aatccaaaaa caaaagagca atatcagcta 60 acaaagaatt ttgatgttat ctggttatgg tccgaagacg gaaaaaactg gtatgaggaa 120 gtgaagaact ttcagccaga cacaataaag attgtttacg atgaaaataa tattattgtc 180 gctatcacca gagatgcttc aacgcttaat cctgaaggtt ttagcgttgt tgaggttcct 240 gatattacct ccaaccgacg tgctgacgac tcaggtaaat ggatgtttaa ggatggtgct 300 gtggttaaac ggatttatac ggcagatgaa cagcaacaac aggcagaatc acaaaaggcc 360 gcgttacttt ccgaagcgga aaacgttatt cagccactgg aacgcgctgt caggctgaat 420 atggcgacgg atgaggaacg tgcacgactg gagtcatggg aacgttacag cgttctggtc 480 agccgtgtgg atcctgcaaa tcctgaatgg ccggaaatgc cgcaa 525 <210> 59 <211> 1158 <212> PRT <213> Artificial Sequence <220> <223> Sequence of Z2145 gpJ of Figure 7 <220> <221> MISC_FEATURE <222> (9) <223> Xaa may be any amino acid <220> <221> MISC_FEATURE <222> (495) <223> Xaa may be any amino acid <220> <221> MISC_FEATURE <222> (813)..(820) <223> box 1 <220> <221> MISC_FEATURE <222> (985)..(993) <223> box 2 <400> 59 Met Gly Lys Gly Gly Gly Lys Gly Xaa Thr Pro Val Glu Ala Lys Asp 1 5 10 15 Asn Leu Lys Ser Thr Gln Met Met Ser Val Ile Asp Ala Ile Gly Glu 20 25 30 Gly Pro Ile Glu Gly Pro Val Lys Gly Leu Gln Ser Ile Leu Val Asn 35 40 45 Lys Thr Pro Leu Thr Asp Thr Asp Gly Asn Pro Val Ile His Gly Val 50 55 60 Thr Ala Val Trp Arg Ala Gly Glu Gln Glu Gln Thr Pro Pro Glu Gly 65 70 75 80 Phe Glu Ser Ser Gly Ala Glu Thr Ala Leu Gly Val Glu Val Thr Lys 85 90 95 Ala Lys Pro Val Thr Arg Thr Ile Thr Ser Ala Asn Ile Asp Arg Leu 100 105 110 Arg Val Thr Phe Gly Val Gln Ser Leu Leu Glu Thr Thr Ser Lys Gly 115 120 125 Asp Arg Asn Pro Ser Ser Val Arg Leu Leu Ile Gln Leu Gln Arg Asn 130 135 140 Gly Asn Trp Val Thr Glu Lys Asp Val Thr Ile Asn Gly Lys Thr Thr 145 150 155 160 Ser Gln Phe Leu Ala Ser Val Ile Leu Asp Asn Leu Pro Pro Arg Pro 165 170 175 Phe Asn Ile Arg Met Val Arg Glu Thr Ala Asp Ser Thr Ser Asp Gln 180 185 190 Leu Gln Asn Lys Thr Leu Trp Ser Ser Tyr Thr Glu Ile Ile Asp Val 195 200 205 Lys Gln Cys Tyr Pro Asn Thr Ala Ile Val Gly Leu Gln Val Asp Ala 210 215 220 Glu Gln Phe Gly Gly Gln Gln Met Thr Val Asn Tyr His Ile Arg Gly 225 230 235 240 Arg Ile Ile Gln Val Pro Ser Asn Tyr Asp Pro Glu Lys Arg Thr Tyr 245 250 255 Ser Gly Ile Trp Asp Gly Ser Leu Lys Pro Ala Tyr Ser Asn Asn Pro 260 265 270 Ala Trp Cys Leu Trp Asp Met Leu Thr His Pro Arg Tyr Gly Met Gly 275 280 285 Lys Arg Leu Gly Ala Ala Asp Val Asp Lys Trp Ala Leu Tyr Ala Ile 290 295 300 Ala Gln Tyr Cys Asp Gln Met Val Pro Asp Gly Phe Gly Gly Thr Glu 305 310 315 320 Pro Arg Met Thr Phe Asn Ala Tyr Leu Ser Gln Gln Arg Lys Ala Trp 325 330 335 Asp Val Leu Ser Asp Phe Cys Ser Ala Met Arg Cys Met Pro Val Trp 340 345 350 Asn Gly Gln Thr Leu Thr Phe Val Gln Asp Ser Pro Ser Asp Val Val 355 360 365 Trp Pro Tyr Thr Asn Ser Asp Val Val Val Asp Asp Asn Gly Val Gly 370 375 380 Phe Arg Tyr Ser Phe Ser Ala Leu Lys Asp Arg His Thr Ala Val Glu 385 390 395 400 Val Asn Tyr Thr Asp Pro Gln Asn Gly Trp Gln Thr Ser Thr Glu Leu 405 410 415 Val Glu Asp Pro Glu Ala Ile Leu Arg Tyr Gly Arg Asn Leu Leu Lys 420 425 430 Met Asp Ala Phe Gly Cys Thr Ser Arg Gly Gln Ala His Arg Ala Gly 435 440 445 Leu Trp Val Ile Lys Thr Gly Leu Leu Glu Thr Gln Thr Val Asp Phe 450 455 460 Thr Leu Gly Ser Gln Gly Leu Arg His Thr Pro Gly Asp Ile Ile Glu 465 470 475 480 Ile Cys Asp Asn Asp Tyr Ala Gly Thr Met Thr Gly Gly Arg Xaa Leu 485 490 495 Ser Ile Asp Ala Ala Ser Arg Thr Leu Thr Leu Asp Arg Glu Val Thr 500 505 510 Leu Pro Glu Thr Gly Ala Ala Thr Val Asn Leu Ile Asn Gly Ser Gly 515 520 525 Lys Pro Val Ser Val Ala Ile Thr Ala His Pro Ala Pro Asp Arg Ile 530 535 540 Gln Val Ser Thr Leu Pro Asp Gly Val Glu Thr Tyr Gly Val Trp Gly 545 550 555 560 Leu Ser Leu Pro Ser Leu Arg Arg Arg Leu Phe Arg Cys Val Ser Val 565 570 575 Arg Glu Asn Thr Asp Gly Thr Phe Ala Ile Thr Ala Val Gln His Val 580 585 590 Pro Glu Lys Glu Ala Ile Val Asp Asn Gly Ala Arg Phe Glu Pro Gln 595 600 605 Ser Gly Thr Leu Asn Ser Val Ile Pro Ala Val Gln His Leu Thr 610 615 620 Val Glu Val Ser Ala Ala Asp Gly Gln Tyr Leu Ala Gln Ala Lys Trp 625 630 635 640 Asp Thr Pro Lys Val Val Lys Gly Val Ser Phe Met Leu Arg Leu Thr 645 650 655 Val Ala Ala Asp Asp Gly Ser Glu Arg Leu Val Ser Thr Ala Arg Thr 660 665 670 Thr Glu Thr Thr Tyr Arg Phe Thr Gln Leu Ala Pro Gly Asn Tyr Arg 675 680 685 Leu Thr Val Arg Ala Val Asn Ala Trp Gly Gln Gln Gly Asp Pro Ala 690 695 700 Ser Val Ser Phe Arg Ile Ala Ala Pro Ala Ala Pro Ser Gln Ile Glu 705 710 715 720 Leu Thr Pro Gly Tyr Phe Gln Ile Thr Ala Val Pro Arg Leu Ala Val 725 730 735 Tyr Asp Pro Thr Val Gln Phe Glu Phe Trp Phe Ser Glu Thr Arg Ile 740 745 750 Thr Asp Ile Arg Gln Val Glu Thr Thr Ala Arg Tyr Leu Gly Thr Gly 755 760 765 Leu Tyr Trp Ile Ala Ala Ser Ile Asn Ile Lys Pro Gly His Asp Tyr 770 775 780 Tyr Phe Tyr Ile Arg Ser Val Asn Thr Val Gly Lys Ser Ala Phe Val 785 790 795 800 Glu Ala Val Gly Gln Pro Ser Asp Asp Ala Ser Gly Tyr Leu Asp Phe 805 810 815 Phe Lys Gly Glu Ile Gly Lys Thr His Leu Ala Gln Glu Leu Trp Thr 820 825 830 Gln Ile Asp Asn Gly Gln Leu Ala Pro Asp Leu Ala Glu Ile Arg Thr 835 840 845 Ser Ile Thr Asp Val Ser Asn Glu Ile Thr Gln Thr Val Asn Lys Lys 850 855 860 Leu Glu Asp Gln Ser Ala Ala Ile Gln Gln Ile Gln Lys Val Gln Val 865 870 875 880 Asp Thr Asn Asn Asn Leu Asn Ser Met Trp Ala Val Lys Leu Gln Gln 885 890 895 Met Gln Asp Gly Arg Leu Tyr Ile Ala Gly Ile Gly Ala Gly Ile Glu 900 905 910 Asn Thr Ser Asp Gly Met Gln Ser Gln Val Leu Leu Ala Ala Asp Arg 915 920 925 Ile Ala Met Ile Asn Pro Ala Asn Gly Asn Thr Lys Pro Met Phe Val 930 935 940 Gly Gln Gly Asp Gln Ile Phe Met Asn Glu Val Phe Leu Lys Tyr Leu 945 950 955 960 Thr Ala Pro Thr Ile Thr Ser Gly Gly Asn Pro Pro Ala Phe Ser Leu 965 970 975 Thr Ser Asp Gly Lys Leu Thr Ala Lys Asn Ala Asp Ile Ser Gly Ser 980 985 990 Val Asn Ala Asn Ser Gly Thr Leu Asn Asn Val Thr Ile Asn Glu Asn 995 1000 1005 Cys Arg Val Leu Gly Lys Leu Ser Ala Asn Gln Ile Glu Gly Asp Leu 1010 1015 1020 Val Lys Thr Val Gly Lys Ala Phe Pro Arg Asp Ser Arg Ala Pro Glu 1025 1030 1035 1040 Arg Trp Pro Ser Gly Thr Ile Thr Val Arg Val Tyr Asp Asp Gln Pro 1045 1050 1055 Phe Asp Arg Gln Ile Val Ile Pro Ala Val Ala Phe Ser Gly Ala Lys 1060 1065 1070 His Glu Arg Glu His Thr Asp Ile Tyr Ser Ser Cys Arg Leu Ile Val 1075 1080 1085 Arg Lys Asn Gly Ala Glu Ile Tyr Asn Arg Thr Ala Leu Asp Asn Thr 1090 1095 1100 Leu Ile Tyr Ser Gly Val Ile Asp Met Pro Ala Gly His Gly His Met 1105 1110 1115 1120 Thr Leu Glu Phe Ser Val Ser Ala Trp Leu Val Asn Asn Trp Tyr Pro 1125 1130 1135 Thr Ala Ser Ile Ser Asp Leu Leu Val Val Val Val Met Lys Lys Ala Thr 1140 1145 1150 Ala Gly Ile Ser Ile Ser 1155 <210> 60 <211> 1131 <212> PRT <213> Artificial Sequence <220> <223> Sequence of 1A2 gpJ of Figure 7 <220> <221> MISC_FEATURE <222> (814)..(821) <223> box 1 <220> <221> MISC_FEATURE <222> (958)..(966) <223> box 2 <400> 60 Met Gly Lys Gly Ser Ser Lys Gly His Thr Pro Arg Glu Ala Lys Asp 1 5 10 15 Asn Leu Lys Ser Thr Gln Leu Leu Ser Val Ile Asp Ala Ile Ser Glu 20 25 30 Gly Pro Val Glu Gly Pro Val Asp Gly Leu Lys Ser Val Leu Leu Asn 35 40 45 Ser Thr Pro Val Leu Asp Ser Glu Gly Asn Thr Asn Ile Ser Gly Val 50 55 60 Thr Val Val Phe Arg Ala Gly Glu Gln Glu Gln Thr Pro Pro Glu Gly 65 70 75 80 Phe Glu Ser Ser Gly Ser Glu Thr Val Leu Gly Thr Glu Val Lys Tyr 85 90 95 Asp Thr Pro Ile Thr Arg Thr Ile Thr Ser Ala Asn Ile Asp Arg Leu 100 105 110 Arg Phe Thr Phe Gly Val Gln Ala Leu Val Glu Thr Thr Ser Lys Gly 115 120 125 Asp Arg Asn Pro Ser Glu Val Arg Leu Leu Val Gln Ile Gln Arg Asn 130 135 140 Gly Gly Trp Val Thr Glu Lys Asp Ile Thr Ile Lys Gly Lys Thr Thr 145 150 155 160 Ser Gln Tyr Leu Ala Ser Val Val Val Gly Asn Leu Pro Pro Arg Pro 165 170 175 Phe Asn Ile Arg Met Arg Arg Met Thr Pro Asp Ser Thr Thr Asp Gln 180 185 190 Leu Gln Asn Lys Thr Leu Trp Ser Ser Tyr Thr Glu Ile Ile Asp Val 195 200 205 Lys Gln Cys Tyr Pro Asn Thr Ala Leu Val Gly Val Gln Val Asp Ser 210 215 220 Glu Gln Phe Gly Ser Gln Gln Val Ser Arg Asn Tyr His Leu Arg Gly 225 230 235 240 Arg Ile Leu Gln Val Pro Ser Asn Tyr Asn Pro Gln Thr Arg Gln Tyr 245 250 255 Ser Gly Ile Trp Asp Gly Thr Phe Lys Pro Ala Tyr Ser Asn Asn Met 260 265 270 Ala Trp Cys Leu Trp Asp Met Leu Thr His Pro Arg Tyr Gly Met Gly 275 280 285 Lys Arg Leu Gly Ala Ala Asp Val Asp Lys Trp Ala Leu Tyr Val Ile 290 295 300 Gly Gln Tyr Cys Asp Gln Ser Val Pro Asp Gly Phe Gly Gly Thr Glu 305 310 315 320 Pro Arg Ile Thr Cys Asn Ala Tyr Leu Thr Thr Gln Arg Lys Ala Trp 325 330 335 Asp Val Leu Ser Asp Phe Cys Ser Ala Met Arg Cys Met Pro Val Trp 340 345 350 Asn Gly Gln Thr Leu Thr Phe Val Gln Asp Arg Pro Ser Asp Lys Val 355 360 365 Trp Thr Tyr Asn Arg Ser Asn Val Val Met Pro Asp Asp Gly Ala Pro 370 375 380 Phe Arg Tyr Ser Phe Ser Ala Leu Lys Asp Arg His Asn Ala Val Glu 385 390 395 400 Val Asn Trp Ile Asp Pro Asn Asn Gly Trp Glu Thr Ala Ala Glu Leu 405 410 415 Val Glu Asp Thr Gln Ala Ile Ala Arg Tyr Gly Arg Asn Val Thr Lys 420 425 430 Met Asp Ala Phe Gly Cys Thr Ser Arg Gly Gln Ala His Arg Ala Gly 435 440 445 Leu Trp Leu Ile Lys Thr Glu Leu Leu Glu Thr Gln Thr Val Asp Phe 450 455 460 Ser Val Gly Ala Glu Gly Leu Arg His Val Pro Gly Asp Val Ile Glu 465 470 475 480 Ile Cys Asp Asp Asp Tyr Ala Gly Ile Ser Ile Gly Gly Arg Val Leu 485 490 495 Ala Val Asn Ser Gln Thr Arg Thr Leu Thr Leu Asp Arg Glu Ile Thr 500 505 510 Leu Pro Phe Ser Gly Thr Thr Leu Ile Ser Leu Val Asp Gly Ser Gly 515 520 525 Asn Pro Val Ser Val Glu Val Gln Ser Val Thr Asp Gly Val Lys Val 530 535 540 Lys Val Ser Arg Val Pro Asp Gly Val Ala Glu Tyr Ser Val Trp Gly 545 550 555 560 Leu Lys Leu Pro Thr Leu Arg Gln Arg Leu Phe Arg Cys Val Ser Ile 565 570 575 Arg Glu Asn Asp Asp Gly Thr Tyr Ala Ile Thr Ala Val Gln His Val 580 585 590 Pro Glu Lys Glu Ala Ile Val Asp Asn Gly Ala His Phe Asp Gly Asp 595 600 605 Gln Ser Gly Thr Val Asn Gly Val Thr Pro Pro Ala Val Gln His Leu 610 615 620 Thr Ala Glu Val Thr Ala Asp Ser Gly Glu Tyr Gln Val Leu Ala Arg 625 630 635 640 Trp Asp Thr Pro Lys Val Val Lys Gly Val Ser Phe Leu Leu Arg Leu 645 650 655 Thr Val Thr Ala Asp Asp Gly Ser Glu Arg Leu Val Ser Thr Ala Arg 660 665 670 Thr Thr Glu Thr Thr Tyr Arg Phe Thr Gln Leu Ala Leu Gly Asn Tyr 675 680 685 Arg Leu Thr Val Arg Ala Val Asn Ala Trp Gly Gln Gln Gly Asp Pro 690 695 700 Ala Ser Val Ser Phe Arg Ile Ala Ala Pro Ala Ala Pro Ser Arg Ile 705 710 715 720 Glu Leu Thr Pro Gly Tyr Phe Gln Ile Thr Ala Thr Pro His Leu Ala 725 730 735 Val Tyr Asp Pro Thr Val Gln Phe Glu Phe Trp Phe Ser Glu Lys Arg 740 745 750 Ile Ala Asp Ile Arg Gln Val Glu Thr Thr Ala Arg Tyr Leu Gly Thr 755 760 765 Gly Leu Tyr Trp Ile Ala Ala Ser Ile Asn Ile Lys Pro Gly His Asp 770 775 780 Tyr Tyr Phe Tyr Ile Arg Ser Val Asn Thr Val Gly Lys Ser Ala Phe 785 790 795 800 Val Glu Ala Val Gly Arg Ala Ser Asp Asp Ala Glu Gly Tyr Leu Asp 805 810 815 Phe Phe Lys Gly Lys Ile Thr Glu Ser His Leu Gly Lys Glu Leu Leu 820 825 830 Glu Lys Val Asp Leu Thr Glu Asp Asn Ala Ser Arg Leu Asp Gln Phe 835 840 845 Ser Lys Glu Trp Lys Asp Ala Asn Asp Lys Trp Asn Ala Met Trp Gly 850 855 860 Val Lys Ile Glu Gln Thr Glu Asp Gly Lys His Tyr Val Ala Gly Leu 865 870 875 880 Gly Leu Ser Met Glu Asp Thr Glu Glu Gly Lys Leu Ser Gln Phe Leu 885 890 895 Val Ala Ala Asn Arg Ile Ala Phe Ile Asp Pro Ser Asn Gly Asn Thr 900 905 910 Arg Pro Met Phe Val Gly Gln Gly Asp Gln Ile Phe Met Asn Asp Val 915 920 925 Phe Leu Lys Arg Leu Thr Ala Pro Thr Ile Thr Ser Gly Gly Asn Pro 930 935 940 Pro Ala Phe Ser Leu Thr Pro Asp Gly Arg Leu Thr Ala Lys Asn Ala 945 950 955 960 Asp Ile Ser Gly Asn Val Asn Ala Asn Ser Gly Thr Leu Asn Asn Val 965 970 975 Thr Ile Asn Glu Asn Cys Arg Val Leu Gly Lys Leu Ser Ala Asn Gln 980 985 990 Ile Glu Gly Asp Leu Val Lys Thr Val Gly Lys Ala Phe Pro Arg Asp 995 1000 1005 Ser Arg Ala Pro Glu Arg Trp Pro Ser Gly Thr Ile Thr Val Arg Val 1010 1015 1020 Tyr Asp Asp Gln Pro Phe Asp Arg Gln Ile Val Ile Pro Ala Val Ala 1025 1030 1035 1040 Phe Ser Gly Ala Lys His Glu Lys Glu His Thr Asp Ile Tyr Ser Ser 1045 1050 1055 Cys Arg Leu Ile Val Arg Lys Asn Gly Ala Glu Ile Tyr Asn Arg Thr 1060 1065 1070 Ala Leu Asp Asn Thr Leu Ile Tyr Ser Gly Val Ile Asp Met Pro Ala 1075 1080 1085 Gly His Gly His Met Thr Leu Glu Phe Ser Val Ser Ala Trp Leu Val 1090 1095 1100 Asn Asn Trp Tyr Pro Thr Ala Ser Ile Ser Asp Leu Leu Val Val Val 1105 1110 1115 1120 Met Lys Lys Ala Thr Ala Gly Ile Thr Ile Ser 1125 1130 <210> 61 <211> 1132 <212> PRT <213> Artificial Sequence <220> <223> Sequence of 591 gpJ of Figure 7 <220> <221> MISC_FEATURE <222> (814)..(821) <223> box 1 <220> <221> MISC_FEATURE <222> (958)..(966) <223> box 2 <400> 61 Met Gly Lys Gly Ser Ser Lys Gly His Thr Pro Arg Glu Ala Lys Asp 1 5 10 15 Asn Leu Lys Ser Thr Gln Leu Leu Ser Val Ile Asp Ala Ile Ser Glu 20 25 30 Gly Pro Val Glu Gly Pro Val Asp Gly Leu Lys Ser Val Leu Leu Asn 35 40 45 Ser Thr Pro Val Leu Asp Ser Glu Gly Asn Thr Asn Ile Ser Gly Val 50 55 60 Thr Val Val Phe Arg Ala Gly Glu Gln Glu Gln Thr Pro Pro Glu Gly 65 70 75 80 Phe Glu Ser Ser Gly Ser Glu Thr Val Leu Gly Thr Glu Val Lys Tyr 85 90 95 Asp Thr Pro Ile Thr Arg Thr Ile Thr Ser Ala Asn Ile Asp Arg Leu 100 105 110 Arg Phe Thr Phe Gly Val Gln Ala Leu Val Glu Thr Thr Ser Lys Gly 115 120 125 Asp Arg Asn Pro Ser Glu Val Arg Leu Leu Val Gln Ile Gln Arg Asn 130 135 140 Gly Gly Trp Val Thr Glu Lys Asp Ile Thr Ile Lys Gly Lys Thr Thr 145 150 155 160 Ser Gln Tyr Leu Ala Ser Val Val Val Gly Ser Leu Pro Pro Arg Pro 165 170 175 Phe Asn Ile Arg Met Arg Arg Met Thr Pro Asp Ser Thr Thr Asp Gln 180 185 190 Leu Gln Asn Lys Thr Leu Trp Ser Ser Tyr Thr Glu Ile Ile Asp Val 195 200 205 Lys Gln Cys Tyr Pro Asn Thr Ala Leu Val Gly Val Gln Val Asp Ser 210 215 220 Glu Gln Phe Gly Ser Gln Gln Val Ser Arg Asn Tyr His Leu Arg Gly 225 230 235 240 Arg Ile Leu Gln Val Pro Ser Asn Tyr Asn Pro Gln Thr Arg Gln Tyr 245 250 255 Ser Gly Ile Trp Asp Gly Thr Phe Lys Pro Ala Tyr Ser Asn Asn Pro 260 265 270 Ala Trp Cys Leu Trp Asp Met Leu Thr His Pro Arg Tyr Gly Met Gly 275 280 285 Lys Arg Leu Gly Ala Ala Asp Val Asp Lys Trp Ala Leu Tyr Val Ile 290 295 300 Gly Gln Tyr Cys Asp Gln Ser Val Pro Asp Gly Phe Gly Gly Thr Glu 305 310 315 320 Pro Arg Ile Thr Cys Asn Ala Tyr Leu Thr Thr Gln Arg Lys Ala Trp 325 330 335 Asp Val Leu Ser Asp Phe Cys Ser Ala Met Arg Cys Met Pro Val Trp 340 345 350 Asn Gly Gln Thr Leu Thr Phe Val Gln Asp Arg Pro Ser Asp Lys Val 355 360 365 Trp Thr Tyr Asn Arg Ser Asn Val Val Met Pro Asp Asp Gly Ala Pro 370 375 380 Phe Arg Tyr Ser Phe Ser Ala Leu Lys Asp Arg His Asn Ala Val Glu 385 390 395 400 Val Asn Trp Ile Asp Pro Asn Asn Gly Trp Glu Thr Ala Thr Glu Leu 405 410 415 Val Glu Asp Thr Gln Ala Ile Ala Arg Tyr Gly Arg Asn Val Thr Lys 420 425 430 Met Asp Ala Phe Gly Cys Thr Ser Arg Gly Gln Ala His Arg Ala Gly 435 440 445 Leu Trp Leu Ile Lys Thr Glu Leu Leu Glu Thr Gln Thr Val Asp Phe 450 455 460 Ser Val Gly Ala Glu Gly Leu Arg His Val Pro Gly Asp Val Ile Glu 465 470 475 480 Ile Cys Asp Asp Asp Tyr Ala Gly Ile Ser Thr Gly Gly Arg Val Leu 485 490 495 Ala Val Asn Ser Gln Thr Arg Thr Leu Thr Leu Asp Arg Glu Ile Thr 500 505 510 Leu Pro Ser Ser Gly Thr Thr Leu Ile Ser Leu Val Asp Gly Ser Gly 515 520 525 Asn Pro Val Ser Val Glu Val Gln Ser Val Thr Asp Gly Leu Lys Val 530 535 540 Lys Val Asn Arg Val Pro Asp Gly Val Ala Glu Tyr Ser Val Trp Gly 545 550 555 560 Leu Lys Leu Pro Thr Leu Arg Gln Arg Leu Phe Arg Cys Val Ser Ile 565 570 575 Arg Glu Asn Asp Asp Gly Thr Tyr Ala Ile Thr Ala Val Gln His Val 580 585 590 Pro Glu Lys Glu Ala Ile Val Asp Asn Gly Ala His Phe Asp Gly Asp 595 600 605 Gln Ser Ser Thr Val Asn Gly Val Thr Pro Pro Ala Val Gln His Leu 610 615 620 Thr Ala Glu Val Thr Ala Asp Ser Gly Glu Tyr Gln Val Leu Ala Arg 625 630 635 640 Trp Asp Thr Pro Lys Val Val Lys Gly Val Ser Phe Leu Leu Arg Leu 645 650 655 Thr Val Thr Ala Asp Asp Gly Ser Glu Arg Leu Val Ser Thr Ala Arg 660 665 670 Thr Thr Glu Thr Thr Tyr Arg Phe Thr Gln Leu Ala Leu Gly Asn Tyr 675 680 685 Arg Leu Thr Val Arg Ala Val Asn Ala Trp Gly Gln Gln Gly Asp Pro 690 695 700 Ala Ser Val Ser Phe Arg Ile Ala Ala Pro Ala Ala Pro Ser Arg Ile 705 710 715 720 Glu Leu Thr Pro Gly Tyr Phe Gln Ile Thr Ala Thr Pro His Leu Ala 725 730 735 Val Tyr Asp Pro Thr Val Gln Phe Glu Phe Trp Phe Ser Glu Lys Arg 740 745 750 Ile Thr Asp Ile Arg Gln Val Glu Thr Thr Ala Arg Tyr Leu Gly Thr 755 760 765 Ala Leu Tyr Trp Ile Ala Ala Ser Ile Asn Ile Lys Pro Gly His Asp 770 775 780 Tyr Tyr Phe Tyr Ile Arg Ser Val Asn Thr Val Gly Lys Ser Ala Phe 785 790 795 800 Val Glu Ala Val Gly Arg Ala Ser Asp Asp Ala Glu Gly Tyr Leu Asp 805 810 815 Phe Phe Lys Gly Lys Ile Thr Glu Ser His Leu Gly Lys Glu Leu Leu 820 825 830 Glu Lys Val Glu Leu Thr Glu Asp Asn Ala Ser Arg Leu Glu Glu Phe 835 840 845 Ser Asn Glu Trp Lys Asp Ala Ser Asp Lys Trp Asn Ala Met Trp Ala 850 855 860 Val Lys Ile Glu Gln Thr Lys Asp Gly Lys His Tyr Val Ala Gly Ile 865 870 875 880 Gly Leu Ser Met Glu Asp Thr Glu Glu Gly Lys Leu Ser Gln Phe Leu 885 890 895 Val Ala Ala Asn Arg Ile Ala Phe Ile Asp Pro Ala Asn Gly Asn Glu 900 905 910 Thr Pro Met Phe Val Ala Gln Gly Asn Gln Ile Phe Met Asn Asp Val 915 920 925 Phe Leu Lys Arg Leu Thr Ala Pro Thr Ile Thr Ser Gly Gly Asn Pro 930 935 940 Pro Ala Phe Ser Leu Thr Pro Asp Gly Lys Leu Thr Ala Lys Asn Ala 945 950 955 960 Asp Ile Ser Gly Asn Val Asn Ala Asn Ser Gly Thr Leu Asn Asn Val 965 970 975 Thr Ile Asn Glu Asn Cys Gln Ile Lys Gly Lys Leu Ser Ala Asn Gln 980 985 990 Ile Glu Gly Asp Ile Val Lys Thr Val Ser Lys Ser Phe Pro Arg Thr 995 1000 1005 Asn Ser Tyr Ala Ser Gly Thr Ile Thr Val Arg Ile Ser Asp Asp Gln 1010 1015 1020 Lys Phe Asp Arg Gln Val Met Ile Pro Val Leu Phe Arg Gly Gly 1025 1030 1035 1040 Lys His Glu Asn Phe Asn Ser Asn Asn Gln Gln Ser Tyr Trp Tyr Ser 1045 1050 1055 Thr Cys Arg Leu Arg Val Thr Arg Asn Gly Gln Glu Ile Phe Asn Gln 1060 1065 1070 Ser Thr Thr Asp Ala Gin Gly Val Phe Ser Ser Val Ile Asp Met Pro 1075 1080 1085 Ala Gly Gln Gly Thr Leu Thr Leu Thr Phe Thr Val Ser Ser Ser Gly 1090 1095 1100 Ala Asn Asn Trp Thr Pro Thr Thr Ser Ile Ser Asp Leu Leu Val Val 1105 1110 1115 1120 Val Met Lys Lys Ser Thr Ala Gly Ile Ser Ile Ser 1125 1130 <210> 62 <211> 859 <212> PRT <213> Artificial Sequence <220> <223> Sequence of E6BTD4-ECOLX gpH of Figure 9A <220> <221> MISC_FEATURE <222> (187)..(208) <223> distal region box <220> <221> MISC_FEATURE <222> (301)..(420) <223> proximal region box <400> 62 Met Ala Glu Pro Val Gly Asp Leu Val Val Asp Leu Ser Leu Asp Ala 1 5 10 15 Ala Arg Phe Asp Glu Gln Met Ala Arg Val Arg Arg His Phe Ser Gly 20 25 30 Thr Glu Ser Asp Ala Lys Lys Thr Ala Ala Val Val Glu Gln Ser Leu 35 40 45 Ser Arg Gln Ala Leu Ala Ala Gln Lys Ala Gly Ile Ser Val Gly Gln 50 55 60 Tyr Lys Ala Ala Met Arg Met Leu Pro Ala Gln Phe Thr Asp Val Ala 65 70 75 80 Thr Gln Leu Ala Gly Gly Gln Ser Pro Trp Leu Ile Leu Leu Gln Gln 85 90 95 Gly Gly Gln Val Lys Asp Ser Phe Gly Gly Met Ile Pro Met Phe Arg 100 105 110 Gly Leu Ala Gly Ala Ile Thr Leu Pro Met Val Gly Ala Thr Ser Leu 115 120 125 Ala Val Ala Thr Gly Ala Leu Ala Tyr Ala Trp Tyr Gln Gly Asn Ser 130 135 140 Thr Leu Ser Asp Phe Asn Lys Thr Leu Val Leu Ser Gly Asn Gln Ala 145 150 155 160 Gly Leu Thr Ala Asp Arg Met Leu Val Leu Ser Arg Ala Gly Gln Ala 165 170 175 Ala Gly Leu Thr Phe Asn Gln Thr Ser Glu Ser Leu Thr Ala Leu Val 180 185 190 Asn Ala Gly Val Arg Gly Gly Glu Gln Phe Glu Ala Ile Ser Gln Ser 195 200 205 Val Ala Arg Phe Ser Ser Ala Ser Gly Val Glu Val Asp Lys Val Ala 210 215 220 Glu Ala Phe Gly Lys Leu Thr Thr Asp Pro Thr Ser Gly Leu Thr Ala 225 230 235 240 Met Ala Arg Gln Phe His Asn Val Thr Ala Glu Gln Ile Ala Tyr Val 245 250 255 Ala Gln Leu Gln Arg Ser Gly Asp Glu Ala Gly Ala Leu Gln Ala Ala 260 265 270 Asn Glu Ala Ala Thr Lys Gly Phe Asp Asp Gln Thr Arg Arg Leu Lys 275 280 285 Glu Asn Met Gly Thr Leu Glu Thr Trp Ala Asp Arg Thr Ala Arg Ala 290 295 300 Phe Lys Ser Met Trp Asp Ser Val Leu Asp Ile Gly Arg Pro Asp Thr 305 310 315 320 Ala Gln Gly Met Leu Glu Lys Ala Glu Lys Ala Phe Asp Glu Ala Asp 325 330 335 Lys Lys Trp Gln Trp Tyr Gln Ser Arg Ser His Arg Arg Gly Lys Thr 340 345 350 Ser Ala Phe Leu Ala Asn Leu Arg Gly Ala Trp Glu Asp Arg Ala Asn 355 360 365 Ala Gln Leu Gly Leu Ser Ala Ala Thr Leu Gln Ala Asp Leu Glu Lys 370 375 380 Ala Arg Glu Met Ala Ala Lys Asp Trp Ala Glu Ser Glu Ala Ser Arg 385 390 395 400 Leu Lys Tyr Thr Glu Glu Ala Gln Lys Ala Tyr Glu Arg Leu Gln Thr 405 410 415 Pro Leu Glu Lys Tyr Thr Ala Arg Gln Glu Glu Leu Asn Lys Ala Leu 420 425 430 Lys Asp Gly Lys Ile Leu Gln Ala Asp Tyr Asn Thr Leu Met Ala Ala 435 440 445 Ala Lys Lys Asp Tyr Glu Ala Thr Leu Lys Lys Pro Lys Gln Ser Gly 450 455 460 Val Lys Val Ser Ala Gly Asp Arg Gln Glu Asp Ser Ala His Ala Ala 465 470 475 480 Leu Leu Thr Leu Gln Ala Glu Leu Arg Thr Leu Glu Lys His Ala Gly 485 490 495 Ala Asn Glu Lys Ile Ser Gln Gln Arg Arg Asp Leu Trp Lys Ala Glu 500 505 510 Ser Gln Phe Ala Val Leu Glu Glu Ala Ala Gln Arg Arg Gln Leu Ser 515 520 525 Ala Gln Glu Lys Ser Leu Leu Ala His Lys Asp Glu Thr Leu Glu Tyr 530 535 540 Lys Arg Gln Leu Ala Ala Leu Gly Asp Lys Val Thr Tyr Gln Glu Arg 545 550 555 560 Leu Asn Ala Leu Ala Gln Gln Ala Asp Lys Phe Ala Gln Gln Gln Arg 565 570 575 Ala Lys Arg Ala Ala Ile Asp Ala Lys Ser Arg Gly Leu Thr Asp Arg 580 585 590 Gln Ala Glu Arg Glu Ala Thr Glu Gln Arg Leu Lys Glu Gln Tyr Gly 595 600 605 Asp Asn Pro Leu Ala Leu Asn Asn Val Met Ser Glu Gln Lys Lys Thr 610 615 620 Trp Ala Ala Glu Asp Gln Leu Arg Gly Ser Trp Met Ala Gly Leu Lys 625 630 635 640 Ser Gly Trp Ser Glu Trp Glu Glu Ser Ala Thr Asp Ser Met Ser Gln 645 650 655 Val Lys Ser Ala Ala Thr Gln Thr Phe Asp Gly Ile Ala Gln Asn Met 660 665 670 Ala Ala Met Leu Thr Gly Ser Glu Gln Asn Trp Arg Ser Phe Thr Arg 675 680 685 Ser Val Leu Ser Met Met Thr Glu Ile Leu Leu Lys Gln Ala Met Val 690 695 700 Gly Ile Val Gly Ser Ile Gly Ser Ala Ile Gly Gly Ala Val Gly Gly 705 710 715 720 Gly Ala Ser Ala Ser Gly Gly Thr Ala Ile Gln Ala Ala Ala Ala Lys 725 730 735 Phe His Phe Ala Thr Gly Gly Phe Thr Gly Thr Gly Gly Lys Tyr Glu 740 745 750 Pro Ala Gly Ile Val His Arg Gly Glu Phe Val Phe Thr Lys Glu Ala 755 760 765 Thr Ser Arg Ile Gly Val Gly Asn Leu Tyr Arg Leu Met Arg Gly Tyr 770 775 780 Ala Thr Gly Gly Tyr Val Gly Thr Pro Gly Ser Met Ala Asp Ser Arg 785 790 795 800 Ser Gln Ala Ser Gly Thr Phe Glu Gln Asn Asn His Val Val Ile Asn 805 810 815 Asn Asp Gly Thr Asn Gly Gln Ile Gly Pro Ala Ala Leu Lys Ala Val 820 825 830 Tyr Asp Met Ala Arg Lys Gly Ala Arg Asp Glu Ile Gln Thr Gln Met 835 840 845 Arg Asp Gly Gly Leu Phe Ser Gly Gly Gly Arg 850 855 <210> 63 <211> 686 <212> PRT <213> Artificial Sequence <220> <223> Lambda-STF29 <400> 63 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Glu Thr Ala Ala Ala Ser Ser Lys Asn Ala Ala Lys Thr Ser 130 135 140 Glu Thr Asn Ala Ala Asn Ser Ala Gln Ala Ala Ala Ala Ser Gln Thr 145 150 155 160 Ala Ser Ala Asn Ser Ala Thr Ala Ala Lys Lys Ser Glu Thr Ser Ala 165 170 175 Lys Asn Ser Glu Thr Ala Thr Lys Ala Ser Glu Lys Asn Ala Lys Ser 180 185 190 Ser Gln Thr Ala Ala Lys Thr Ser Glu Thr Asn Ala Lys Asp Ser Glu 195 200 205 Ala Asn Ala Lys Val Ser Glu Thr Ala Ala Ala Asn Ser Ala Lys Ala 210 215 220 Ser Ala Ala Ser Gln Thr Ala Ala Lys Ala Ser Glu Asp Ala Ala Arg 225 230 235 240 Glu Tyr Ala Asn Gln Thr Ala Glu Pro Tyr Arg Tyr Val Leu Gln Pro 245 250 255 Leu Pro Asp Val Trp Ile Pro Phe Asn Asp Ser Leu Asp Met Ile Thr 260 265 270 Gly Tyr Ser Pro Gly Tyr Lys Lys Val Lys Ile Gly Asp Asn Val Val 275 280 285 Gln Val Ala Ser Asp Lys Gln Val Asn Phe Ser Arg Ala Ser Thr Ala 290 295 300 Thr Tyr Ile Asn Lys Ser Gly Glu Leu Lys Thr Ala Glu Ile Asn Glu 305 310 315 320 Pro Arg Phe Glu Lys Glu Gly Leu Leu Ile Glu Gly Gln Arg Thr Asn 325 330 335 Tyr Met Leu Asn Ser Ala Thr Pro Ala Ser Trp Gly Lys Ser Ala Asn 340 345 350 Met Asn Val Ala Glu Val Gly Thr Asp Ser Phe Gly Phe Thr Tyr Gly 355 360 365 Lys Phe Val Cys Asn Glu Ser Leu Ile Gly Gln Ser Thr Thr Leu Asn 370 375 380 Met Ala Val Val Ser Thr Ser Gly Ala Val Asp Val Ser Gly Asp Asn 385 390 395 400 Lys Cys Val Thr Thr Ser Cys Arg Phe Lys Thr Asp Leu Glu Leu Leu 405 410 415 Leu Arg Ile Arg Phe Glu Ala Phe Asp Gly Ser Ala Ser Ser Asn Leu 420 425 430 Gly Tyr Ala Ile Val Asn Thr Arg Ser Leu Leu Val Glu Ile Thr Gly 435 440 445 Val Ala Ala Asp Arg Leu Thr Ala Arg Val Asn Lys Asp Glu Ala Thr 450 455 460 Gly Trp Ile Phe Val Glu Ala Thr Ile Gln Ala Ser Lys Glu Thr Tyr 465 470 475 480 Ile Thr Ser Ala Ile Gln Tyr Ala Pro Lys Lys Gly Gly Val Val Glu 485 490 495 Ser Gly Asp Tyr Ile Tyr Leu Ala Thr Pro Gln Val Glu Asp Gly Ser 500 505 510 Cys Val Ser Ser Phe Ile Ile Ser Gly Thr Thr Ala Ala Thr Arg Ala 515 520 525 Ser Asp Met Val Thr Val Pro Ile Lys Asn Asn Leu Tyr Asn Leu Pro 530 535 540 Phe Thr Val Leu Cys Glu Val His Lys Asn Trp Tyr Lys Thr Pro Asn 545 550 555 560 Ala Ala Pro Arg Val Phe Asp Thr Gly Gly His Gln Thr Gly Ala Ala 565 570 575 Ile Ile Leu Gly Phe Gly Ser Ser Ala Asp Gly Pro Asp Gly Phe Pro 580 585 590 Tyr Cys Asp Ile Gly Gly Ser Asn Arg Arg Val Asn Glu Asn Ala Ser 595 600 605 Leu Lys Lys Met Val Met Gly Met Arg Val Lys Ser Asp Gln Ser Thr 610 615 620 Cys Ala Val Ser Asn Gly Arg Ile Ser Ser Glu Thr Lys Thr Thr Trp 625 630 635 640 Glu Tyr Ile Arg Ser Thr Ala Thr Ile Arg Ile Gly Gly Gln Thr Thr 645 650 655 Ala Gly Leu Arg His Leu Phe Gly His Val Arg Asn Phe Arg Leu Trp 660 665 670 His Lys Glu Leu Thr Asp Ala Gln Leu Gly Glu Val Val Glu 675 680 685 <210> 64 <211> 824 <212> PRT <213> Artificial Sequence <220> <223> Lambda-STF118 <400> 64 Met Ala Val Lys Ile Ser Gly Val Leu Lys Asp Gly Thr Gly Lys Pro 1 5 10 15 Val Gln Asn Cys Thr Ile Gln Leu Lys Ala Arg Arg Asn Ser Thr Thr 20 25 30 Val Val Val Asn Thr Val Gly Ser Glu Asn Pro Asp Glu Ala Gly Arg 35 40 45 Tyr Ser Met Asp Val Glu Tyr Gly Gln Tyr Ser Val Ile Leu Gln Val 50 55 60 Asp Gly Phe Pro Ser His Ala Gly Thr Ile Thr Val Tyr Glu Asp 65 70 75 80 Ser Gln Pro Gly Thr Leu Asn Asp Phe Leu Cys Ala Met Thr Glu Asp 85 90 95 Asp Ala Arg Pro Glu Val Leu Arg Arg Leu Glu Leu Met Val Glu Glu 100 105 110 Val Ala Arg Asn Ala Ser Val Val Ala Gln Ser Thr Ala Asp Ala Lys 115 120 125 Lys Ser Ala Gly Asp Ala Ser Ala Ser Ala Ala Gln Val Ala Ala Leu 130 135 140 Val Thr Asp Ala Thr Asp Ser Ala Arg Ala Ala Ser Thr Ser Ala Gly 145 150 155 160 Gln Ala Ala Ser Ser Ala Gln Glu Ala Ser Ser Gly Ala Glu Ala Ala 165 170 175 Ser Ala Lys Ala Thr Glu Ala Glu Lys Ser Ala Ala Ala Ala Glu Ser 180 185 190 Ser Lys Asn Ala Ala Ala Thr Ser Ala Gly Ala Ala Lys Thr Ser Glu 195 200 205 Thr Asn Ala Ala Ala Ser Gln Gln Ser Ala Ala Thr Ser Ala Ser Thr 210 215 220 Ala Ala Thr Lys Ala Ser Glu Ala Ala Thr Ser Ala Arg Asp Ala Val 225 230 235 240 Ala Ser Lys Glu Ala Ala Lys Ser Ser Glu Thr Asn Ala Ser Ser Ser 245 250 255 Ala Gly Arg Ala Ala Ser Ser Ala Thr Ala Ala Glu Asn Ser Ala Arg 260 265 270 Ala Ala Lys Thr Ser Glu Thr Asn Ala Arg Ser Ser Glu Thr Ala Ala 275 280 285 Glu Arg Ser Ala Ser Ala Ala Ala Asp Ala Lys Thr Ala Ala Ala Gly 290 295 300 Ser Ala Ser Thr Ala Ser Thr Lys Ala Thr Glu Ala Ala Gly Ser Ala 305 310 315 320 Val Ser Ala Ser Gln Ser Lys Ser Ala Ala Glu Ala Ala Ala Ile Arg 325 330 335 Ala Lys Asn Ser Ala Lys Arg Ala Glu Asp Ile Ala Ser Ala Val Ala 340 345 350 Leu Glu Asp Ala Asp Thr Thr Arg Lys Gly Ile Val Gln Leu Ser Ser 355 360 365 Ala Thr Asn Ser Thr Ser Glu Thr Leu Ala Ala Thr Pro Lys Ala Val 370 375 380 Lys Val Val Met Asp Glu Thr Asn Arg Lys Ala Pro Leu Asn Ser Pro 385 390 395 400 Ala Leu Thr Gly Thr Pro Thr Thr Pro Thr Ala Arg Gln Gly Thr Asn 405 410 415 Asn Thr Gln Ile Ala Asn Thr Ala Phe Val Met Ala Ala Ile Ala Ala 420 425 430 Leu Val Asp Ser Ser Pro Asp Ala Leu Asn Thr Leu Asn Glu Leu Ala 435 440 445 Ala Ala Leu Gly Asn Asp Pro Asn Phe Ala Thr Thr Met Thr Asn Ala 450 455 460 Leu Ala Gly Lys Gln Pro Lys Asp Ala Thr Leu Ala Ala Leu Ala Gly 465 470 475 480 Leu Ala Thr Ala Ala Asp Arg Phe Pro Tyr Phe Thr Gly Asn Asp Val 485 490 495 Ala Ser Leu Ala Thr Leu Thr Lys Val Gly Arg Asp Ile Leu Ala Lys 500 505 510 Ser Thr Val Ser Ala Val Ile Glu Tyr Leu Gly Leu Gln Glu Thr Val 515 520 525 Asn Arg Ala Gly Asn Ala Val Gln Lys Asn Gly Asp Thr Leu Ser Gly 530 535 540 Gly Leu Thr Phe Glu Asn Asp Ser Ile Leu Ala Trp Ile Arg Asn Thr 545 550 555 560 Asp Trp Ala Lys Ile Gly Phe Lys Asn Asp Ala Asp Gly Asp Thr Asp 565 570 575 Ser Tyr Met Trp Phe Glu Thr Gly Asp Asn Gly Asn Glu Tyr Phe Lys 580 585 590 Trp Arg Ser Arg Gln Ser Thr Thr Thr Lys Asp Leu Met Asn Leu Lys 595 600 605 Trp Asp Ala Leu Tyr Val Leu Val Lys Ala Leu Phe Ser Ser Glu Val 610 615 620 Lys Ile Ser Thr Val Asn Ala Leu Arg Ile Phe Asn Ser Ser Phe Gly 625 630 635 640 Ala Ile Phe Arg Arg Ser Glu Glu Asn Leu Tyr Ile Ile Pro Thr Arg 645 650 655 Glu Asn Glu Gly Glu Asn Gly Asp Ile Gly Pro Leu Arg Pro Phe Gly 660 665 670 Ile Asn Leu Arg Thr Gly Val Val Ser Val Gly Asn Gly Ala Arg Ile 675 680 685 Asp Gly Gly Leu Ala Leu Gly Thr Asn Asn Ala Leu Gly Gly Asn Ser 690 695 700 Ile Val Leu Gly Asp Asn Asp Thr Gly Phe Lys Gln Asn Gly Asp Gly 705 710 715 720 Asn Leu Asp Val Tyr Ala Asn Asn Val His Val Met Arg Phe Val Ser 725 730 735 Gly Ser Ile Gln Ser Asn Lys Thr Ile Asn Ile Thr Gly Arg Val Asn 740 745 750 Pro Ser Asp Tyr Gly Asn Phe Asp Ser Arg Tyr Val Arg Asp Ile Arg 755 760 765 Leu Gly Thr Arg Val Val Gln Thr Met Gln Lys Gly Val Met Tyr Glu 770 775 780 Lys Ala Gly His Val Ile Thr Gly Leu Gly Ile Val Gly Glu Val Asp 785 790 795 800 Gly Asp Asp Pro Ala Val Phe Arg Pro Ile Gln Lys Tyr Ile Asn Gly 805 810 815 Thr Trp Tyr Asn Val Ala Gln Val 820 <210> 65 <211> 92 <212> PRT <213> Artificial Sequence <220> <223> Lambda-STF29 accessory protein <400> 65 Val Arg Asp Phe Thr Leu Arg Phe Ser Asp Lys Ala Asp Phe Arg Ala 1 5 10 15 Phe Leu Arg Lys Leu Asn Trp Glu Glu Asp Glu Glu Leu Gln Asn Ala 20 25 30 Ile Leu Val Asp Glu Ile Gly Phe Thr Phe Ser Glu Ser Gly Val Ser 35 40 45 Ala Asp Gly Glu Pro Glu Tyr Thr Arg Asp Glu Gly Tyr Phe Val Asn 50 55 60 Ile Arg Leu Leu Asp Asp Gly Phe Asp Glu Ser Val Phe Arg Glu Trp 65 70 75 80 Val Val Thr Pro Glu Arg Pro Leu Arg Glu Trp Phe 85 90 <210> 66 <211> 175 <212> PRT <213> Artificial Sequence <220> <223> Lambda-STF118 accessory protein <400> 66 Met Gln His Leu Lys Asn Ile Thr Ala Gly Asn Pro Lys Thr Val Ala 1 5 10 15 Gln Tyr Gln Leu Thr Lys Asn Phe Asp Val Ile Trp Leu Trp Ser Glu 20 25 30 Glu Gly Lys Asn Trp Tyr Glu Glu Val Ser Asn Phe Gin Glu Asp Thr 35 40 45 Ile Lys Ile Val Tyr Asp Glu Asn Asn Ile Ile Val Gly Ile Thr Arg 50 55 60 Asp Ala Ser Thr Leu Asn Pro Glu Gly Phe Ser Val Val Glu Val Pro 65 70 75 80 Asp Ile Thr Ser Asn Arg Arg Ala Asp Asp Ser Gly Lys Trp Met Phe 85 90 95 Lys Asp Gly Ala Val Ile Lys Arg Ile Tyr Thr Ala Asp Glu Gln Glu 100 105 110 Gln Gln Ala Glu Ser Gln Lys Ala Ala Leu Leu Ser Glu Ala Glu Ser 115 120 125 Val Ile Leu Pro Leu Glu Arg Ala Val Arg Leu Asn Met Ala Thr Asp 130 135 140 Glu Glu Arg Ser Arg Leu Glu Ala Trp Glu Arg Tyr Ser Val Leu Val 145 150 155 160 Ser Arg Val Asp Pro Ala Asn Pro Glu Trp Pro Glu Met Pro Gln 165 170 175

Claims (49)

A lambda-type bacterial delivery vehicle for use in the in vivo delivery of a DNA payload of interest to a targeted bacterial cell. The bacterial delivery vehicle of claim 1 , wherein the bacterial delivery vehicle is a bacteriophage. 3. The bacterial delivery vehicle of claim 1 or 2, wherein the bacterial delivery vehicle is a bacteriophage comprising a wild-type lateral tail fiber (STF) protein, a wild-type gpH protein and a wild-type gpJ protein. 4. The bacterial delivery vehicle according to any one of claims 1 to 3, wherein the bacterial delivery vehicle is a wild-type bacteriophage. The bacterial delivery vehicle of claim 1 , wherein the bacterial delivery vehicle is a packaged phagemid. The bacterium according to any one of claims 1 to 5, comprising at least one protein selected from the group consisting of a functional lambda-type bacteriophage STF protein, a functional lambda-type bacteriophage gpJ protein and a functional lambda-type bacteriophage gpH protein. delivery vehicle. The bacterial delivery vehicle according to claim 6, comprising two or more proteins selected from the group consisting of a functional lambda-type STF protein, a functional lambda-type bacteriophage gpJ protein and a functional lambda-type bacteriophage gpH protein. 8. The method according to any one of claims 1 to 7,
(i) a functional lambda-type bacteriophage STF protein, and
(ii) functional lambda-type bacteriophage gpJ protein
A bacterial delivery vehicle comprising: 9. The method of claim 8,
(iii) functional lambda-type bacteriophage gpH protein
The bacterial delivery vehicle further comprising a. 10. The bacterial delivery vehicle according to any one of claims 3 to 9, wherein the STF protein, gpJ protein and/or gpH protein is a wild-type lambda STF, gpJ and/or gpH protein. 10. The bacterial delivery vehicle according to any one of claims 6 to 9, wherein the STF protein, gpJ protein and/or gpH protein is a non-naturally occurring recombinant protein. The bacterial delivery vehicle of claim 11 , wherein the recombinant STF protein is a chimeric protein comprising a fusion between a portion of the STF protein derived from a lambda-type bacteriophage and a portion of the STF protein derived from the corresponding STF protein from a different bacteriophage. 13. The bacterial delivery according to claim 11 or 12, wherein the recombinant gpJ protein is a chimeric protein comprising a fusion between a portion of a gpJ protein derived from a lambda-type bacteriophage and a portion of a gpJ protein derived from a corresponding gpJ protein from a different bacteriophage. vehicle. 14. The method according to any one of claims 11 to 13, wherein the recombinant gpH protein comprises a fusion between a portion of a gpH protein derived from a lambda-type bacteriophage and a portion of a gpH protein derived from a corresponding gpH protein from a different bacteriophage. Bacterial delivery vehicle that is a chimeric protein. 15. The method according to any one of claims 11 to 14, wherein the STF protein is the amino acid sequence of SEQ ID NO: 14, the amino acid sequence of SEQ ID NO: 16, the amino acid sequence of SEQ ID NO: 17, SEQ ID NO: 19 A bacterial delivery vehicle comprising or consisting of the amino acid sequence of, the amino acid sequence of SEQ ID NO: 21, the amino acid sequence of SEQ ID NO: 44, or the amino acid sequence of SEQ ID NO: 50. 16. The bacterial delivery vehicle according to any one of claims 11 to 15, wherein the gpJ protein comprises or consists of the amino acid sequence of SEQ ID NO: 10, 11, 12, 13 or 49. 17. The bacterial delivery vehicle according to any one of claims 11 to 16, wherein the gpH protein comprises or consists of the amino acid sequence of SEQ ID NO: 23 or 24. 18. The bacterial delivery vehicle according to any one of claims 11 to 17, wherein the recombinant STF protein, gpJ protein and/or gpH protein is engineered to allow delivery of a DNA payload of interest to a targeted bacterial cell. 19. The bacterial delivery vehicle according to any one of claims 11 to 18, wherein the recombinant STF protein has an enzymatic activity such as a depolymerase activity and the targeted bacterial cell is an encapsulated bacterial cell. 20. The bacterial delivery vehicle according to any one of claims 11 to 19, wherein the recombinant STF protein, gpJ protein and/or gpH protein is engineered to increase the efficiency of delivery of the DNA payload to the targeted bacterial cell. 21. The method of any one of claims 1-20, wherein the targeted bacterial cell is Yersinia spp. , Escherichia spp., Klebsiella spp., Acinetobacter spp., Pseudomonas spp., Helicobacter spp., Vibrio spp, Vibrio Salmonella spp., Streptococcus spp., Staphylococcus spp., Bacteroides spp., Clostridium spp., Shigella spp., Selected from the group consisting of Enterococcus spp., Enterobacter spp., and Listeria spp , preferably E. Bacterial delivery vehicle that is E. coli . 22. The bacterial delivery vehicle of any one of claims 1-21, wherein the bacterial delivery vehicle comprises the DNA payload of interest. 23. The method of any one of claims 1-22, wherein the DNA payload is a Cas nuclease gene, a Cas9 nuclease gene, a guide RNA, a CRISPR locus, a toxin gene, an enzyme such as a nuclease or kinase, TALEN, A gene encoding a ZFN, a meganuclease, a recombinase, a bacterial receptor, a membrane protein, a structural protein, a secreted protein, a gene encoding resistance to an antibiotic or over-the-counter drug, a gene encoding a toxic protein or a virulence factor, and a nucleic acid of interest selected from the group consisting of a gene encoding a virulence protein or virulence factor, and any combination thereof. 24. The bacterial delivery vehicle of claim 23, wherein the nucleic acid of interest is a gene encoding a nuclease. 25. The bacterial delivery vehicle of claim 24, wherein the nuclease is selected from the group consisting of Cas nucleases, Cas9 nucleases, TALENs, ZFNs and meganucleases. 26. The bacterial delivery vehicle of claim 24 or 25, wherein the nuclease targets cleavage of a host bacterial cell chromosome or a host bacterial cell plasmid. 27. The bacterial delivery vehicle of any one of claims 24-26, wherein the cleavage occurs in an antibiotic resistance gene. 28. The method of any one of claims 1-27, wherein the DNA payload has a size strictly superior to 10.000 kb and strictly inferior to 12.000 kb, or strictly superior to 12.500 kb and strictly inferior to 16.667 kb. A bacterial delivery vehicle having an inferior size, or having a size of at least 18.000 kb and no more than 25.000 kb. 27. The bacterial delivery vehicle of any one of claims 1-26, wherein the DNA payload comprises or consists of the sequence SEQ ID NO: 47. 27. The method according to any one of claims 1 to 26, comprising a recombinant STF protein comprising or consisting of the sequence SEQ ID NO: 48 and a recombinant gpJ protein comprising or consisting of the sequence SEQ ID NO: 13, The DNA payload comprises or consists of the sequence SEQ ID NO: 47, wherein the targeted bacterial cell is Shiga-toxin producing E. coli (STEC), a bacterial delivery vehicle. 23. The bacterial delivery vehicle of any one of claims 1-22, wherein the DNA payload comprises a nucleic acid of interest encoding a therapeutic protein. 23. The bacterial delivery vehicle of any one of claims 1-22, wherein the DNA payload comprises a nucleic acid of interest encoding an antisense nucleic acid molecule. 33. A pharmaceutical or veterinary composition comprising the bacterial delivery vehicle of any one of claims 1-32 and a pharmaceutically acceptable carrier. A method for in vivo delivery of a DNA payload of interest to a subject, comprising administering to the subject the pharmaceutical or veterinary composition of claim 33 . A method for treating a disease or disorder caused by a bacterium, comprising administering the pharmaceutical or veterinary composition of claim 33 to a subject having a disease or disorder caused by said bacterium in need thereof. Way. 36. The method of claim 35, wherein the disease or disorder is a bacterial infection, a metabolic disorder or a condition involving bacteria of the human microbiome. 37. The method of claim 36, wherein the bacterial infection is a STEC infection. 34. A pharmaceutical or veterinary composition according to claim 33 for use in treating a disease or disorder caused by bacteria. 39. The pharmaceutical or veterinary composition of claim 38, wherein said disease or disorder is a condition involving bacterial infection, metabolic disorder or bacteria of the human microbiome. 40. The pharmaceutical or veterinary composition of claim 39, wherein the bacterial infection is a STEC infection. 33. A method for reducing the amount of virulent and/or antibiotic resistant bacteria in a bacterial population comprising the step of contacting the bacterial population with the bacterial delivery vehicle of any one of claims 1-32. 33. The bacterial delivery vehicle according to any one of claims 1 to 32, for use in a method for reducing the amount of virulent and/or antibiotic resistant bacteria in a bacterial population. 40. The pharmaceutical or veterinary composition of claim 38 or 39, wherein the bacterial delivery vehicle is used to reduce the amount of virulent and/or antibiotic resistant bacteria in the targeted bacterial cell population. 33. A bacterial delivery vehicle according to any one of claims 1 to 32 for use in treating a disease or disorder caused by bacteria. 45. The bacterial delivery vehicle of claim 44, wherein the bacterial delivery vehicle is used for in vivo delivery of a DNA payload of interest to a targeted bacterial cell population. 46. The bacterial delivery vehicle of claim 44 or 45, wherein said disease or disorder is a condition involving bacterial infection, metabolic disorder or bacteria of the human microbiome. 47. The bacterial delivery vehicle of claim 46, wherein said bacterial infection is a STEC infection. 33. A production cell line for producing a bacterial delivery vehicle as defined in any one of claims 1 to 32. 49. The method of claim 48, wherein the production cell line
- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 14 and an associated chaperone thereof comprising or consisting of the amino acid sequence of SEQ ID NO: 15,
- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 16,
- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 17 and an associated chaperone thereof comprising or consisting of the amino acid sequence of SEQ ID NO: 18,
- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 19 and an associated chaperone thereof comprising or consisting of the amino acid sequence of SEQ ID NO: 20,
- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 21 and an associated chaperone thereof comprising or consisting of the amino acid sequence of SEQ ID NO: 22,
- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 44, or
- a STF protein comprising or consisting of the amino acid sequence of SEQ ID NO: 50 and optionally an associated chaperone thereof comprising or consisting of the amino acid sequence of SEQ ID NO: 57
A production cell line that produces

Images