KR20220104403A - Functional feed composition for poultry containing sophora koreensis extract as an active ingredient and a method for producing the same - Google Patents
Functional feed composition for poultry containing sophora koreensis extract as an active ingredient and a method for producing the same Download PDFInfo
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- KR20220104403A KR20220104403A KR1020210006619A KR20210006619A KR20220104403A KR 20220104403 A KR20220104403 A KR 20220104403A KR 1020210006619 A KR1020210006619 A KR 1020210006619A KR 20210006619 A KR20210006619 A KR 20210006619A KR 20220104403 A KR20220104403 A KR 20220104403A
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- poultry
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- feed
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S426/00—Food or edible material: processes, compositions, and products
- Y10S426/807—Poultry or ruminant feed
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Abstract
Description
본 발명은 개느삼(sophora koreensis) 추출물을 유효성분으로 포함하는 가금용 기능성 사료 조성물 및 이의 제조방법에 관한 것이다.The present invention relates to a functional feed composition for poultry comprising an extract of sophora koreensis as an active ingredient and a method for preparing the same.
한국의 식품 소비는 소득수준의 향상과 건강에 대한 관심의 증가로 1980년대부터 질적인 성장단계로 전환되었다. 이는 식품 자체의 품질도 중요하지만, 도시로 인구가 집중되면서 식품 소비의 간소화와 저장성이 높은 식품에 대한 선호도가 증가하고 있다. 또한 곡물 위주의 소비에서 육류 위주의 소비로 전환되었으며, 육류의 소비량은 지속적으로 증가하고 있다. 특히 닭고기는 실제 도계수수가 2000년 연간 약 4억 수에서 2020년 10억 수까지 증가할 만큼 지속적인 성장을 하였으며, 저렴하면서도 저지방, 저칼로리, 고단백 식품으로써 건강식 및 다이어트 식품으로써 각광받으며 그 소비량은 꾸준히 증가하고 있다. 육계의 소비량 증가와 더불어 토종닭에 대한 관심 또한 증가하는 상황으로 토종닭은 육계에 비하여 지방함량이 낮고 담백하며, 육질이 단단하여 아미노산 및 핵산물이 다량 함유되어 있어 일반 육계보다 기호성이 좋은 것으로 알려져 있다. 하지만 육계 보다 긴 사육기간과 높은 가격으로 인해 소비자들이 쉽게 접하지 못하는 실정이다. 따라서 토종닭 사양에 있어 사육기간을 단축시키거나 증체량을 증가시키는 방안이 요구되고 있다.Food consumption in Korea has shifted to a qualitative growth stage since the 1980s due to an increase in income level and increased interest in health. Although the quality of food itself is important, as the population is concentrated in cities, preference for food with high storability and simplification of food consumption is increasing. In addition, the consumption of grain-centered consumption has shifted to meat-centered consumption, and the consumption of meat is continuously increasing. In particular, chicken has continued to grow so that the actual slaughter count has increased from about 400 million per year in 2000 to 1 billion by 2020. are doing As the consumption of broilers increases, interest in native chickens is also increasing. Compared to broilers, native chickens have a lower fat content and are lighter, and their meat is firmer and contains a large amount of amino acids and nucleic acids. have. However, due to the longer breeding period and higher price than broilers, consumers cannot easily access them. Therefore, there is a need for a method to shorten the breeding period or increase the weight gain in breeding native chickens.
공급량을 증가시키기 위해서는 단위면적 당 사육수수를 증가시키는 것이 가장 효과적이나, 고밀도 사육의 경우 사료 및 음수경쟁으로 인해 사회적 스트레스로 인하여 문제가 되고 있다. 이러한 스트레스는 닭의 사양성적 감소와 더불어 골격근 발달 저하 및 지방축적의 증가를 유발한다고 알려져 있다. 또한 열악한 사육환경에 의한 산화적 스트레스가 유발되어 단백질 및 지방세포의 구조와 기능을 파괴하는 등의 문제가 발생되었다. Free radical을 조절할 수 있는 물질로 항산화제의 개발 연구가 활발히 진행 중이다. 산화적 스트레스를 완화하기 위해 항산화제로써 주로 비타민 E 혹은 합성 항산화제를 첨가하는데, 비타민 E는 경제적 측면에서 사용이 제한적이며 합성 항산화제는 안전성의 문제가 제기되었다. 이러한 안전성 문제로 천연 식물이 항산화제로써 이용되는 연구가 진행 중이다. 천연 식물유래 사료첨가제는 대부분이 항산화 기능을 가지고 있는데, Jamroz 등 (2005)은 항산화 활성을 위한 식물체를 가금류의 사료 첨가제로 사용 시 사료섭취량의 증가 및 면역력이 증가한다는 연구결과가 보고되어 있다. 또한 닭고기에 항산화제로써 포도씨 추출물을 첨가 시 지질 과산화물 생성과 육색의 변색이 방지되었다는 연구 보고도 있다. 또한 개똥쑥 분말을 육계 사료 내 첨가 시 지질과산화물 함량이 감소하고, 혈액의 항산화 활성이 유의적으로 증가하는 등의 효과가 나타났지만 그 기전은 명확히 밝혀지지 않았다.In order to increase the supply, it is most effective to increase the number of breeding per unit area, but in the case of high-density breeding, it becomes a problem due to social stress due to competition for feed and drinking water. It is known that such stress causes a decrease in skeletal muscle development and an increase in fat accumulation along with a decrease in the breeding performance of chickens. In addition, oxidative stress was induced by the poor breeding environment, causing problems such as destroying the structure and function of proteins and fat cells. Research on the development of antioxidants as substances that can control free radicals is being actively conducted. Vitamin E or synthetic antioxidants are mainly added as antioxidants to relieve oxidative stress, but vitamin E has limited use in economic terms, and the safety of synthetic antioxidants has been raised. Due to these safety concerns, studies are underway to use natural plants as antioxidants. Most of the natural plant-derived feed additives have antioxidant functions, and Jamroz et al. (2005) reported that the use of plants for antioxidant activity as a feed additive for poultry increases feed intake and immunity. There is also a study report that the addition of grape seed extract as an antioxidant to chicken prevented the formation of lipid peroxide and discoloration of the meat. In addition, when wormwood powder was added to broiler feed, effects such as a decrease in lipid peroxide content and a significant increase in blood antioxidant activity were found, but the mechanism was not clearly elucidated.
한편, 개느삼(Sophora koreensis)은 쌍떡잎식물 장미목 콩과의 낙엽 관목으로 한국의 특산식물로 분류되고, 강원도 양구와 인제, 이북지방의 평안남도, 함경남도 등지에 분포한다. 개느삼은 항균효과 및 항산화 효과가 우수한 알칼로이드와 플라보노이드 성분들을 함유하고 있는 것으로 알려져 있다. 또한 강력한 항산화물질인 스코폴레틴(scopoletin)이 함유되어 있으며, 스코폴레틴(scopoletin)은 쿠마린(coumarin) 계열의 물질로 노니와 개똥쑥 등의 식물성 항산화제 원료의 주성분이다. 하지만 개느삼은 멸종위기야생동식물 Ⅱ급으로 지정되어 보호되고 있었고 2012년 해제되었으나 개체 수가 매우 적고 재배법이 개발되지 않아 개느삼에 관련한 연구는 매우 부족한 실정이며 특히 개느삼이 육계의 사료로써 연구된 사례는 없었다. 따라서 본 연구에서는 사료 내 개느삼 첨가 시 토종닭의 도체특성, 혈액성상, 육질 및 항산화 활성을 조사하였다.On the other hand, Sophora koreensis is a deciduous shrub of the family Rosaceae, a dicotyledonous plant, classified as a Korean endemic plant, and distributed in Yanggu and Inje, Gangwon-do, and South Pyongan Province and South Hamgyong Province in the northern region. Ginseng is known to contain alkaloids and flavonoids with excellent antibacterial and antioxidant effects. It also contains scopoletin, a powerful antioxidant, and scopoletin, a coumarin-based substance, is the main component of plant antioxidants such as noni and wormwood. However, ginseng was designated as an endangered wild plant and animal class II and protected, and although it was released in 2012, the number of individuals was very small and the cultivation method was not developed. Therefore, in this study, carcass characteristics, blood characteristics, meat quality and antioxidant activity of native chickens were investigated when ginseng was added to the feed.
즉, 본 발명에서는 개느삼(sophora koreensis) 추출물을 유효성분으로 포함하는 가금용 기능성 사료 조성물 및 이의 제조방법에 관한 것으로 본 발명의 개느삼 추출물을 가금류 사료에 첨가하는 경우 토종닭의 혈청분석에서 항산화 기능이 증진되고, 계육의 품질도 일반사료를 먹인 것보다 향상됨을 확인하였다. 즉, 이러한 결과를 토대로 개느삼 추출물을 유효성분으로 포함하는 가금용 기능성 사료 조성물로 사용될 수 있을 것이다.That is, the present invention relates to a functional feed composition for poultry comprising an extract of sophora koreensis as an active ingredient and a method for preparing the same. It was confirmed that the quality of chicken meat was also improved compared to that fed with general feed. That is, based on these results, it will be able to be used as a functional feed composition for poultry containing the ginseng extract as an active ingredient.
상기와 같은 문제점을 해결하기 위한 본 발명의 목적은, 개느삼 추출물을 유효성분으로 포함하는 가금류 품질 개선용 기능성 사료 조성물을 제공하는 것이다.It is an object of the present invention to solve the above problems, to provide a functional feed composition for improving the quality of poultry comprising an extract of ginseng as an active ingredient.
또한, 본 발명의 다른 목적은 개느삼 추출물을 유효성분으로 포함하는 가금류 품질 개선용 기능성 사료 첨가제를 제공하는 것이다.In addition, another object of the present invention is to provide a functional feed additive for improving the quality of poultry comprising an extract of ginseng as an active ingredient.
상기 목적을 달성하기 위하여, 본 발명은 개느삼 추출물을 유효성분으로 포함하는 가금류 품질 개선용 기능성 사료 조성물을 제공한다.In order to achieve the above object, the present invention provides a functional feed composition for improving the quality of poultry comprising an extract of ginseng extract as an active ingredient.
본 발명의 일 실시예에 있어서, 상기 ‘추출물’은 물 또는 유기용매 추출법으로 추출되는 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the 'extract' may be characterized in that it is extracted by water or organic solvent extraction method, but is not limited thereto.
본 발명의 일 실시예에 있어서, 상기 ‘유기용매 추출법’은 60 내지 80% 에탄올을 이용한 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the 'organic solvent extraction method' may be characterized by using 60 to 80% ethanol, but is not limited thereto.
본 발명의 일 실시예에 있어서, 상기 ‘조성물’은 가금류의 복강 내 지방을 감소시키는 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the 'composition' may be characterized in that it reduces the fat in the abdominal cavity of poultry, but is not limited thereto.
본 발명의 일 실시예에 있어서, 상기 ‘조성물’은 가금류의 비장, F낭 및 갑상선의 무게를 증가시켜 면역 기능을 향상시키는 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the 'composition' may be characterized in that it increases the weight of the poultry's spleen, F sac and thyroid gland to improve immune function, but is not limited thereto.
본 발명의 일 실시예에 있어서, 상기 ‘조성물’은 가금류의 보수력, 가열감량, 전단력 및 pH를 개선시키는 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the 'composition' may be characterized by improving the water holding capacity, heating loss, shear force and pH of poultry, but is not limited thereto.
본 발명의 일 실시예에 있어서, 상기 ‘조성물’은 가금류 혈청의 MDA, SOD 및 TA를 증가시키는 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the 'composition' may be characterized by increasing the MDA, SOD and TA of poultry serum, but is not limited thereto.
본 발명의 일 실시예에 있어서, 상기 ‘조성물’은 가금류 다리육의 SOD, TA 및 CAT를 증가시키는 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the 'composition' may be characterized by increasing the SOD, TA and CAT of poultry leg meat, but is not limited thereto.
본 발명의 일 실시예에 있어서, 상기 ‘조성물’은 가금류의 혈청 및 다리육의 ABTS+ 소거능을 증가시키는 것을 특징으로 할 수 있으나 이에 한정되는 것은 아니다.In one embodiment of the present invention, the 'composition' may be characterized in that it increases the ABTS + scavenging ability of poultry serum and leg meat, but is not limited thereto.
또한, 본 발명은 개느삼 추출물을 유효성분으로 포함하는 가금류 품질 개선용 기능성 사료 첨가제에서, 상기 추출물은 물 또는 유기용매 추출법으로 추출되는 것을 특징으로 하고, 상기 사료 첨가제는 가금류의 복강 내 지방을 감소시키는 것을 특징으로 하며, 상기 사료 첨가제는 가금류의 비장, F낭 및 갑상선의 무게를 증가시켜 면역 기능을 향상시키는 것을 특징으로 하고, 상기 사료 첨가제는 가금류의 보수력, 가열감량, 전단력 및 pH를 개선시키는 것을 특징으로 하며, 상기 사료 첨가제는 가금류 혈청의 MDA, SOD 및 TA를 증가시키는 것을 특징으로 하고, 상기 사료 첨가제는 가금류 다리육의 SOD, TA 및 CAT를 증가시키는 것을 특징으로 하며, 상기 사료 첨가제는 가금류의 혈청 및 다리육의 ABTS+ 소거능을 증가시키는 것을 특징으로 하는, 가금류 품질 개선용 기능성 사료 첨가제를 제공한다.In addition, the present invention is a functional feed additive for improving the quality of poultry comprising a ginseng extract as an active ingredient, wherein the extract is extracted by water or an organic solvent extraction method, and the feed additive reduces intra-abdominal fat of poultry. Characterized in that, the feed additive improves immune function by increasing the weight of the poultry's spleen, F sac and thyroid gland, and the feed additive improves the water holding capacity, heating loss, shearing force and pH of poultry Characterized in that, the feed additive is characterized in that it increases the MDA, SOD and TA of poultry serum, the feed additive is characterized in that it increases the SOD, TA and CAT of poultry leg meat, the feed additive is poultry It provides a functional feed additive for improving poultry quality, characterized in that it increases the ABTS + scavenging ability of serum and leg meat.
본 발명은 개느삼(sophora koreensis) 추출물을 유효성분으로 포함하는 가금용 기능성 사료 조성물 및 이의 제조방법에 관한 것으로 본 발명의 개느삼 추출물을 가금류 사료에 첨가하는 경우 토종닭의 혈청분석에서 항산화 기능이 증진되고, 계육의 품질도 일반사료를 먹인 것보다 향상됨을 확인하였다. 즉, 이러한 결과를 토대로 개느삼 추출물을 유효성분으로 포함하는 가금용 기능성 사료 조성물로 유용하게 이용될 수 있을 것이다.The present invention relates to a functional feed composition for poultry comprising an extract of sophora koreensis as an active ingredient and a method for preparing the same. , it was confirmed that the quality of chicken meat was also improved compared to that fed with general feed. That is, based on these results, it will be usefully used as a functional feed composition for poultry containing the extract of ginseng extract as an active ingredient.
도 1은 본 발명의 사료내 개느삼 첨가에 따른 가슴육의 지방함량 변화를 나타낸 도이다(a, 개느삼 0ppm처리; b, 개느삼 10ppm처리; c, 개느삼 20ppm처리; d, 개느삼 30ppm처리).
도 2는 본 발명의 사료내 개느삼 첨가에 따른 다리육의 지방함량 변화를 나타낸 도이다(a, 개느삼 0ppm처리; b, 개느삼 10ppm처리; c, 개느삼 20ppm처리; d, 개느삼 30ppm처리).
도 3은 토종닭 사료 내 개느삼의 수준별 첨가가 혈청과 다리육에서의 ABTS+ 소거능 분석 결과를 나타낸 도이다.1 is a diagram showing changes in the fat content of breast meat according to the addition of ginseng in the feed of the present invention (a, ginseng 0ppm treatment; b, ginseng 10ppm treatment; c, ginseng ginseng 20ppm treatment; d, ginseng ginseng 30ppm treatment).
2 is a diagram showing the change in the fat content of leg meat according to the addition of ginseng in the feed of the present invention (a, ginseng 0ppm treatment; b, ginseng 10ppm treatment; c, ginseng ginseng 20ppm treatment; d, ginseng ginseng 30ppm treatment).
3 is a diagram showing the results of analysis of ABTS + scavenging ability in serum and leg meat of each level of ginseng added in native chicken feed.
이하, 첨부된 도면을 참조하여 본 발명의 구현예로 본 발명을 상세히 설명하기로 한다. 다만, 하기 구현 예는 본 발명에 대한 예시로 제시되는 것으로, 당업자에게 주지 저명한 기술 또는 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명을 생략할 수 있고, 이에 의해 본 발명이 제한되지는 않는다. 본 발명은 후술하는 특허 청구범위의 기재 및 그로부터 해석되는 균등 범주 내에서 다양한 변형 및 응용이 가능하다.Hereinafter, the present invention will be described in detail by way of embodiments of the present invention with reference to the accompanying drawings. However, the following embodiments are presented as examples of the present invention, and when it is determined that detailed descriptions of well-known techniques or configurations known to those skilled in the art may unnecessarily obscure the gist of the present invention, the detailed description may be omitted, and , the present invention is not limited thereby. Various modifications and applications of the present invention are possible within the scope of equivalents interpreted therefrom and the description of the claims to be described later.
또한, 본 명세서에서 사용되는 용어(terminology)들은 본 발명의 바람직한 실시 예를 적절히 표현하기 위해 사용된 용어들로서, 이는 사용자, 운용자의 의도 또는 본 발명이 속하는 분야의 관례 등에 따라 달라질 수 있다. 따라서 본 용어들에 대한 정의는 본 명세서 전반에 걸친 내용을 토대로 내려져야 할 것이다. 명세서 전체에서, 어떤 부분이 어떤 구성요소를 “포함”한다고 할 때, 이는 특별히 반대되는 기재가 없는 한 다른 구성요소를 제외하는 것이 아니라 다른 구성 요소를 더 포함할 수 있는 것을 의미한다.In addition, the terms used in this specification are terms used to properly express a preferred embodiment of the present invention, which may vary according to the intention of a user or operator, or a custom in the field to which the present invention belongs. Therefore, definitions of these terms should be made based on the content throughout this specification. Throughout the specification, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.
본 명세서 전체에 걸쳐, 특정 물질의 농도를 나타내기 위하여 사용되는 '%'는 별도의 언급이 없는 경우, 고체/고체는(w/w) %, 고체/액체는(w/v) %, 그리고 액체/액체는(v/v) %이다.Throughout this specification, '%' used to indicate the concentration of a specific substance is solid/solid (w/w) %, solid/liquid (w/v) %, and Liquid/liquid is (v/v) %.
일 측면에서, 본 발명은 개느삼 추출물을 유효성분으로 포함하는 가금용 기능성 사료 조성물을 제공한다.In one aspect, the present invention provides a functional feed composition for poultry comprising a ginseng extract as an active ingredient.
본 발명의 사료 조성물은 토종닭의 혈청분석에서 항산화 기능이 증진되고, 계육의 품질도 일반사료를 먹인 것보다 향상됨을 확인 하였다. 본 발명의 사료 조성물은 발효사료, 배합사료, 펠렛 형태 및 사일레지 등의 형태로 제조될 수 있다. 상기 발효사료는 본 발명의 펩타이드 이외의 여러 가지 미생물군 또는 효소들을 첨가함으로서 유기물을 발효시켜 제조할 수 있으며, 배합사료는 여러 종류의 일반사료와 본 발명의 펩타이드를 혼합하여 제조할 수 있다. 펠렛 형태의 사료는 상기 배합사료 등을 펠렛기에서 열과 압력을 가하여 제조할 수 있으며, 사일레지는 청예 사료를 본 발명에 따른 미생물로 발효시킴으로써 제조할 수 있다. 습식발효사료는 음식물 쓰레기 등과 같은 유기물을 수집 및 운반하여 살균과정과 수분조절을 위한 부형제를 일정비율로 혼합한 후, 발효에 적당한 온도에서 24시간 이상 발효하여, 수분함량이 약 70%으로 포함되도록 조절하여 제조할 수 있다. 발효건조사료는 습식 발효 사료를 건조과정을 추가로 거쳐 수분함량이 30% 내지 40% 정도 함유되도록 조절하여 제조할 수 있다.It was confirmed that the feed composition of the present invention improved the antioxidant function in the serum analysis of native chickens, and the quality of chicken was also improved compared to that fed with general feed. The feed composition of the present invention may be prepared in the form of fermented feed, compound feed, pellet form, silage, and the like. The fermented feed can be prepared by fermenting organic matter by adding various microbial groups or enzymes other than the peptide of the present invention, and the compounded feed can be prepared by mixing several types of general feed with the peptide of the present invention. The feed in the form of pellets may be prepared by applying heat and pressure to the compounded feed, etc. in a pellet machine, and silage may be prepared by fermenting the cheongye feed with the microorganism according to the present invention. Wet fermented feed collects and transports organic matter such as food waste, mixes excipients for sterilization process and moisture control in a certain ratio, and ferments it at a temperature suitable for fermentation for 24 hours or more so that the moisture content is about 70%. It can be adjusted and manufactured. Fermented dry feed can be prepared by adjusting the moisture content of the wet fermented feed to 30% to 40% through an additional drying process.
본 발명의 사료 조성물은 종래 사료에 첨가되는 성분을 더 포함할 수 있다. 이러한 사료에 첨가되는 성분의 일예로서 곡류분말, 고기분말, 및 두류 등을 포함할 수 있다. 상기에서 곡류분말은 쌀가루, 밀가루, 보리가루, 및 옥수수가루 중에서 선택된 1종 이상을 사용할 수 있다. 상기에서 고기분말은 닭고기, 소고기, 돼지고기, 및 타조고기 중에서 선택된 어느 하나 이상을 분말화한 고기분말을 사용할 수 있다. 상기에서 두류는 대두, 강낭콩, 완두콩, 및 검정콩 중에서 선택된 1종 이상을 사용할 수 있다.The feed composition of the present invention may further include a component added to a conventional feed. Examples of the ingredients added to the feed may include cereal powder, meat powder, and beans. In the above, the grain powder may be one or more selected from rice flour, wheat flour, barley flour, and corn flour. In the above, the meat powder may be a meat powder obtained by pulverizing any one or more selected from chicken, beef, pork, and ostrich meat. In the above-mentioned beans, one or more selected from soybeans, kidney beans, peas, and black beans may be used.
본 발명의 사료 조성물은 상기에서 언급한 종래 사료에 첨가되는 성분인 곡류분말, 고기분말, 및 두류 이외에도 사료의 영양성을 증대시키기 위해 영양제, 및 무기물 중에서 선택된 어느 하나 이상을 첨가할 수 있으며, 사료 품질의 저하를 막기 위해 항곰팡이제, 항산화제, 항응고제, 유화제, 및 결착제 중에서 선택된 1종 이상을 포함할 수 있다.In the feed composition of the present invention, any one or more selected from nutrients and inorganic substances may be added to the feed composition in addition to the above-mentioned ingredients added to conventional feed, such as grain powder, meat powder, and beans, to increase the nutritional value of the feed, and feed quality It may include at least one selected from an antifungal agent, an antioxidant, an anticoagulant, an emulsifier, and a binder in order to prevent the deterioration.
이하, 실시예를 이용하여 본 발명을 더욱 상세하게 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로 본 발명의 범위가 이들에 의해 제한되지 않는다는 것은 당해 기술분야에서 통상의 지식을 가진 자에게 있어 자명한 것이다.Hereinafter, the present invention will be described in more detail using examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited thereto.
준비예 1. 개느삼(Preparation Example 1. ginseng ( sophora koreensissophora koreensis ) 추출물의 제조) Preparation of extract
개느삼의 지상부(잎 및 줄기)를 70% 에탄올 10배수를 가하여 24시간 동안 상온에서 침지하여 추출한 후 여과지(Watman No.1)를 이용하여 필터한 후 감압원심농축기를 이용하여 40℃에서 농축하여 유기용매를 제거하였다. 농축된 시료는 동결건조기로 건조하여 분말상으로 제조하여 사료에 첨가하였다.After adding 10 times 70% ethanol to the above-ground parts (leaf and stem) of ginseng, 10 times 70% ethanol was added, extracted by immersion at room temperature for 24 hours, filtered using filter paper (Watman No. The solvent was removed. The concentrated sample was dried with a freeze dryer to form a powder and added to the feed.
준비예 2. 시험설계, 공시동물 및 사양관리Preparation Example 2. Test design, test animals and breeding management
본 연구는 강원대학교 동물실험윤리위원회(Institutional Animal care and use committee, Kangwon National University, Korea)의 승인을 받아 수행하였다. 사료 내 개느삼 첨가수준(0, 10, 20, 30ppm)에 따른 효과를 알아보기 위해 4처리 6반복, 반복 당 13수 씩 총 312수의 토종닭(수컷, 1914 ±1.7g, 49일령)을 공시하여 총 35일간 사양실험을 수행하였다(표 1). 시험 사료는 가루 형태이며, 영양소 수준은 한국 사양표준에서 제시된 영양소 요구량을 충족하거나 초과하도록 배합하였다(표 2).This study was conducted with the approval of the Institutional Animal care and use committee, Kangwon National University, Korea. In order to examine the effect according to the level of ginseng added in the feed (0, 10, 20, 30ppm), 4 treatments, 6 repetitions, 13 numbers per repetition, a total of 312 native chickens (male, 1914 ±1.7g, 49 days of age) were disclosed. Thus, the specification experiment was performed for a total of 35 days (Table 1). The test feed was in the form of powder, and the nutrient level was formulated to meet or exceed the nutrient requirements suggested in the Korean specification standard (Table 2).
본 시험에 사용된 개느삼을 제외하고는 다른 첨가제나 약품은 사용하지 않았다. 시험 사료는 자유급이를 하였으며, 물은 니플을 통하여 자유롭게 마실 수 있게 하였다. 점등은 시험 기간 동안 간헐점등(1L:2D) 하였으며, 조도는 10∼15lux로 조정하였다. 시험 기간 중 계사 내 평균온도는 24℃로 유지하였고, 습도는 평균 약 70%를 유지하였다. 기타 사양 관리는 본 연구실의 관행에 준하여 실시하였다.Except for the ginseng used in this test, no other additives or drugs were used. The test food was fed ad libitum, and water was freely drinkable through the nipple. Lighting was performed intermittently (1L:2D) during the test period, and the illuminance was adjusted to 10~15lux. During the test period, the average temperature in the house was maintained at 24°C, and the average humidity was maintained at about 70%. Management of other specifications was carried out according to the practice of this laboratory.
실험예 1. 도체특성Experimental Example 1. Conductor Characteristics
70일령에 처리 당 6수 씩 무작위로 선정 후 경추 탈골을 통해 도계하여 방혈한 뒤 깃털과 다리를 제거한 후 도체특성 및 상대적 장기 무게를 조사하였다. 도체율은 내장을 제거한 도체를 생체중으로 나누어 백분율로 환산하여 계산하였고, 가슴육 및 다리육, 복강 내 지방, 간, 비장, F낭 및 갑상선을 적출 한 뒤 생체중으로 나누어 백분율로 환산하여 표현하였다.At 70 days of age, 6 animals per treatment were randomly selected, slaughtered through cervical dislocation, exsanguinated, feathers and legs were removed, and carcass characteristics and relative organ weights were investigated. The carcass percentage was calculated by dividing the carcass from which the intestines were removed by the live weight and converted into a percentage.
토종닭 사료 내 개느삼의 수준별 첨가가 도체특성에 미치는 영향은 표 2와 같다. 도체성적의 경우 개느삼 첨가에 따른 유의적인 차이는 발견되지 않았다(p<0.05). 하지만 복강 내 지방은 개느삼 첨가수준에 따라 유의적으로 감소하였으며(p>0.05), 20ppm을 첨가한 처리구에서 그 효과가 가장 높게 나타났다. 상대적 장기 무게의 경우 개느삼의 수준별 첨가가 비장, F낭 및 갑상선의 상대적 무게를 증가시키고(p<0.05), 비장/F낭의 비율에도 유의적인 차이가 나타났다(p<0.05). 하지만 토종닭 사료 내 개느삼의 첨가에 따른 간의 무게에는 영향을 미치지 않는 것으로 나타났다(p<0.05).Table 2 shows the effects of the addition of ginseng by level in the feed for native chickens on carcass characteristics. In case of carcass performance, no significant difference was found according to the addition of ginseng ( p< 0.05). However, intra-abdominal fat was significantly decreased according to the level of ginseng addition ( p >0.05), and the effect was highest in the treatment group added with 20 ppm. In the case of relative organ weight, the addition of ginseng by level increased the relative weights of the spleen, F sac, and thyroid gland ( p <0.05), and there was a significant difference in the ratio of spleen/F sac ( p <0.05). However, it was found that the addition of ginseng in native chicken feed did not affect the liver weight ( p <0.05).
비장과 F낭은 육계의 주요 면역 관련 기관으로써 무게가 증가할수록 면역기능이 향상되는 것으로 알려져 있다. 비장/F낭의 비율은 가금에 있어서 면역 억제를 나타내는 지표로, 비장이 더 클 경우, 면역 억제상태로써 호흡기 전염병 등 발생이 쉽게 나타난다. 갑상선은 갑상선 호르몬을 분비하여 체내 호르몬 대사를 조절하는 기관이며, 체내 산화작용을 촉진시켜 가축의 성장과 체내 가슴 및 복강 내 지방축적과도 밀접한 관계를 가지고 있다. Selye 등(1951)은 가축이 스트레스 요인에 강하게 장기간 주어졌을 때 갑상선, 비장, F낭의 무게가 감소한다고 보고하였다. 본 실험에서 개느삼의 첨가는 비장과 F낭의 무게를 증가시켰으며, 이는 개느삼의 첨가가 토종닭의 면역력 강화에 긍정적인 영향을 미치는 것으로 사료된다.The spleen and F sac are the main immune-related organs of broilers and are known to improve immune function as their weight increases. The ratio of spleen/F cyst is an indicator of immunosuppression in poultry, and if the spleen is larger, respiratory infections, etc. easily appear as an immunosuppressed state. The thyroid gland is an organ that regulates hormone metabolism in the body by secreting thyroid hormone. Selye et al. (1951) reported that the weight of the thyroid gland, spleen, and F sac decreased when livestock was given a strong and prolonged stressor. In this experiment, the addition of ginseng increased the weight of the spleen and F sac, which is thought to have a positive effect on strengthening the immunity of native chickens.
실험예 2. 혈액성상Experimental Example 2. Blood Characteristics
실험 종료일에 익하정맥에서 혈액 3ml를 SST 튜브에 채취 후 2,500rpm, 4℃에서 15분 간 원심분리하여 상층액을 분리하였다. 분석 전까지 -80℃에서 보관하였다. 자동 혈액 분석기(AU480, Beckman Coulter, Japan)를 이용하여 총 콜레스테롤, 단백질, 중성지방, 글루코스, GPT, GOT, 알부민, 인 및 칼슘을 분석하였고, 혈청 내 스트레스 호르몬인 콜티코스테론 농도는 corticosterone ELISA kit(Mybiosource inc, USA)를 이용해 측정하였다.On the end of the experiment, 3 ml of blood was collected from the subdylar vein in an SST tube, and the supernatant was separated by centrifugation at 2,500 rpm and 4° C. for 15 minutes. Stored at -80°C until analysis. Total cholesterol, protein, triglyceride, glucose, GPT, GOT, albumin, phosphorus and calcium were analyzed using an automatic blood analyzer (AU480, Beckman Coulter, Japan). (Mybiosource inc, USA) was used.
토종닭 사료 내 개느삼의 수준별 첨가가 혈액성상에 미치는 영향은 표 3과 같다. 개느삼의 첨가에 따른 혈액성상의 변화는 유의적인 차이가 나타나지 않았다.Table 3 shows the effect of the addition of ginseng by level in the native chicken feed on blood properties. There was no significant difference in the change of blood properties according to the addition of ginseng.
실험예 3. 육질분석Experimental Example 3. Meat quality analysis
3-1. pH3-1. pH
pH 측정은 Kim 등(2018)의 방법에 따라 다리육 10g에 증류수 90ml를 가한 후 pH를 측정하였다. 모든 시료는 Homogenizer(Bihon seiki, Ace, Japan)로 7,000rpm으로 30초간 균질시킨 후, pH meter(Orion 230A, Thermo Fisher Scientific, Inc., Waltham, MA, USA)로 측정하였다.The pH was measured after adding 90 ml of distilled water to 10 g of leg meat according to the method of Kim et al. (2018). All samples were homogenized for 30 seconds at 7,000 rpm with a homogenizer (Bihon seiki, Ace, Japan), and then measured with a pH meter (Orion 230A, Thermo Fisher Scientific, Inc., Waltham, MA, USA).
3-2. 보수력(Water holding capacity)3-2. Water holding capacity
보수력은 Jang 등(2011)의 방법에 따라 시험관에 근막을 제거한 다리육을 약 0.5 g 측량하여 80℃의 항온수조에서 20분간 가열하였다. 가열 후 10분동안 실온에서 방냉하였으며, 2,000×g에서 20분간 원심분리한 다음 시료의 무게를 측정하였다. 보수력은 다음의 수학식 1을 이용하여 계산하였다.According to the method of Jang et al. (2011), about 0.5 g of leg meat with fascia removed was measured in a test tube and heated in a constant temperature water bath at 80° C. for 20 minutes. After heating, it was allowed to cool at room temperature for 10 minutes, centrifuged at 2,000 × g for 20 minutes, and then the weight of the sample was measured. The water holding capacity was calculated using Equation 1 below.
[수학식 1][Equation 1]
보수력(%) = [(총 수분 - 유리수분)/총 수분] × 100Water holding capacity (%) = [(total moisture - free moisture)/total moisture] × 100
유리수분 = [(원심분리 전 무게 - 원심분리 후 무게)/(시료×지방계수)] × 100Free moisture = [(weight before centrifugation - weight after centrifugation)/(sample × fat coefficient)] × 100
지방계수 = 1 - (지방함량)/100Fat coefficient = 1 - (fat content)/100
3-3. 가열감량(Cooking loss)3-3. Cooking loss
가열감량은 Kim 등(2017)의 방법에 따라 시험관에 근막을 제거한 다리육을 약 0.5g 측량하여 80℃의 항온수조에서 45분간 가열하였다. 가열감량 값은 가열 전*?*후의 중량 차이를 백분율로 하여 계산하였다(수학식 2).According to the method of Kim et al. (2017), about 0.5 g of leg meat with fascia removed was weighed in a test tube and heated in a constant temperature water bath at 80° C. for 45 minutes. The heating loss value was calculated as a percentage of the weight difference before *?* after heating (Equation 2).
[수학식 2][Equation 2]
가열감량(%) = (가열 전 무게 - 가열 후 무게)/가열 전 무게 × 100Loss on heating (%) = (weight before heating - weight after heating)/weight before heating × 100
3-4. 육색3-4. six colors
가슴육의 육색은 Minolta Chromameter CR-300(Minolta Camera Co., Ltd., Osaka, Japan)를 사용하여 명도 값(Lightness)을 나타내는 L*값, 적색도(Redness)를 나타내는 a*값과 황색도(Yellowness)를 나타내는 b*값을 각각 3회 반복하며 측정하였다. Minolta chroma meter의 표준화 작업은 표준색판(Y = 92.8, x = 0.3134, y = 0.3193)을 이용하였다.The color of the breast meat was determined by using a Minolta Chromameter CR-300 (Minolta Camera Co., Ltd., Osaka, Japan) to determine the L * value indicating the lightness, the a * value indicating the redness, and the yellowness (Yellowness) b * value was measured by repeating each three times. For standardization of the Minolta chroma meter, a standard color plate (Y = 92.8, x = 0.3134, y = 0.3193) was used.
3-5. 실험결과3-5. Experiment result
토종닭 사료 내 개느삼의 수준별 첨가가 가슴육의 육색과 다리육의 육질에 미치는 영향은 표 4, 도 1 및 도 2와 같다. 개느삼의 첨가에 따른 가슴육 육색의 변화는 유의적인 차이가 나타나지 않았다(p<0.05). 하지만 개느삼의 첨가에 따른 다리육의 육질 변화의 경우, 개느삼의 첨가에 따라 보수력, 가열감량, 전단력 및 pH가 개선되는 것으로 나타났다(p<0.05).Table 4, Figs. 1 and 2 show the effects of the addition of ginseng by level in native chicken feed on the color of breast meat and meat quality of leg meat. There was no significant difference in the color change of breast meat according to the addition of ginseng ( p <0.05). However, in the case of changes in the meat quality of leg meat according to the addition of ginseng, water holding capacity, heating loss, shearing force and pH were improved according to the addition of ginseng ( p <0.05).
보수력은 외부 자극으로부터 수분을 유지하는 능력으로(Trout, 1988), pH가 낮을수록 보수력이 감소하고 가열감량이 증가한다는 보고가 있었는데(Northcutt 등, 1994; Palansk 와 Nosal, 1991), 이는 본 실험의 결과와 일치했다.Water holding capacity is the ability to retain moisture from external stimuli (Trout, 1988), and it has been reported that the lower the pH, the lower the water holding capacity and increase the loss on heating (Northcutt et al., 1994; Palansk and Nosal, 1991). consistent with the results.
실험예 4. 혈청 및 다리육 항산화 활성도 조사Experimental Example 4. Investigation of antioxidant activity of serum and leg meat
혈청 및 다리육 항산화 활성도를 알아보기 위해 Malondialdehyde(MDA), Superoxide dismutase(SOD), Catalase(CAT) 및 Total antioxidant(TA) 를 조사하였다. 먼저 근막이 제거된 다리육을 약 5g을 채취하여 15mL의 PBS와 균질 후 분석에 이용하였다. 혈청과 균질된 다리육 내 MDA측정은 Buege 와 Aust(1978)의 방법을 이용하여 진행하였다. 균질된 다리육 및 혈청 1mL에 50μL 7.2% BHA를 넣고 산화반응을 정지시킨 후 2mL의 20mM 2-thiobarbituric acid(15% trichloroacetic acid) 시약 첨가 후, 항온수조 85℃에서 15분간 가열하였다. 가열 후 얼음 위에서 10분간 정치 후 원심분리기를 이용하여 2,000×g 로 10분간 원심 분리하였다. 원심 분리한 시료의 상층을 회수하여 531nm에서 흡광도를 측정하여 TBARS 값을 계산하였다. SOD, CAT 및 TA는 각각 Cayman 사의 kit(SOD: 706002; CAT: 707002; TA: 709001)를 이용해 분석하였다.Malondialdehyde (MDA), Superoxide dismutase (SOD), Catalase (CAT), and Total antioxidant (TA) were investigated to determine the antioxidant activity of serum and leg meat. First, about 5 g of leg meat from which the fascia was removed was used for analysis after homogenization with 15 mL of PBS. MDA measurement in serum and homogenized leg meat was performed using the method of Buege and Aust (1978). 50 μL of 7.2% BHA was added to 1 mL of homogenized leg meat and serum, and after stopping the oxidation reaction, 2 mL of 20 mM 2-thiobarbituric acid (15% trichloroacetic acid) reagent was added, followed by heating at 85° C. in a constant temperature water bath for 15 minutes. After heating and standing on ice for 10 minutes, centrifugation was performed at 2,000 × g for 10 minutes using a centrifuge. The upper layer of the centrifuged sample was recovered and absorbance was measured at 531 nm to calculate the TBARS value. SOD, CAT, and TA were analyzed using Cayman's kit (SOD: 706002; CAT: 707002; TA: 709001), respectively.
토종닭 사료 내 개느삼의 수준별 첨가가 혈청과 다리육에서 항산화 활성도 분석의 결과는 표 5와 같다. 혈청의 경우, 개느삼의 첨가에 따라 MDA, SOD 및 TA가 유의적으로 증가하였다(p>0.05). 그러나 CAT의 경우 유의적인 차이가 나타나지 않았다(p<0.05). 다리육의 경우, 개느삼의 첨가에 따라 MDA가 감소하고 SOD 및 TA가 유의적으로 증가하였다(p>0.05). CAT의 경우 유의적인 차이는 나타나지 않았지만, 개느삼의 첨가가 CAT의 활성을 증가시키는 경향이 나타났다(p=0.078).Table 5 shows the results of analysis of antioxidant activity in serum and leg meat added by level of ginseng in native chicken feed. In the case of serum, MDA, SOD and TA were significantly increased with the addition of ginseng ( p >0.05). However, there was no significant difference in CAT (p<0.05). In the case of leg meat, MDA decreased and SOD and TA significantly increased with the addition of ginseng ( p >0.05). In the case of CAT, there was no significant difference, but the addition of ginseng showed a tendency to increase the activity of CAT ( p = 0.078).
활성산소종이 세포막과 반응하면서 지질과산화가 발생하며 그 결과로 MDA가 생성된다. 이는 세포막과 조직의 손상을 유발하여 세포의 노화 및 세포 파괴 등의 주된 요인이며, 수치가 증가할수록 지방의 산패 정도를 나타낸다. 한 등(2012)은 육계에 뽕잎을 첨가한 연구결과에서 혈액 내 MDA가 유의적으로 감소하였으며, 김 등(2014)은 야콘 부산물 분말과 쑥 분말을 육계 사료에 첨가한 실험에서 계육 내 MDA가 유의적으로 감소한 결과가 있었는데, 본 실험에서도 개느삼 추출물 첨가 사료를 먹인 계육 내 MDA가 유의적으로 감소하였다.. SOD는 항산화 효소의 일종으로 세포 내 활성산소종을 과산화수소로 전환시키는 반응을 촉매하는 효소이다. SOD로 인해 생성된 과산화수소는 CAT에 의해 무해한 물분자로 분해됨으로 유해산소로부터 생체를 보호하는 것으로 알려져 있다. 김 등(2015)의 식물의 대사산물인 quercetin을 육계 사료 내 첨가한 실험에서 혈액 내 SOD 유사 활성이 유의적으로 증가하였는데 본 실험에서도 SOD 유사활성이 증가하였다.As reactive oxygen species react with the cell membrane, lipid peroxidation occurs, and as a result, MDA is produced. This causes damage to cell membranes and tissues, and is a major factor in cell aging and cell destruction. Han et al. (2012) found that MDA in the blood was significantly reduced in the study result of adding mulberry leaves to broilers, and Kim et al. (2014) found that MDA in chicken meat was significantly reduced in an experiment in which yacon by-product powder and mugwort powder were added to broiler feed. In this experiment, MDA was significantly decreased in chickens fed with ginseng extract-added feed. SOD is an antioxidant enzyme that catalyzes the conversion of intracellular reactive oxygen species to hydrogen peroxide. . Hydrogen peroxide generated by SOD is decomposed into harmless water molecules by CAT and is known to protect the living body from harmful oxygen. Kim et al. (2015) showed that SOD-like activity in blood was significantly increased in an experiment in which quercetin, a metabolite of plants, was added to broiler feed, and this experiment also increased SOD-like activity.
실험예 5. ABTSExperimental Example 5. ABTS ++ 소거활성 scavenging activity
ABTS+ 측정방법은 Kim 등(2013)의 방법으로, ABTS의 양이온 라디칼의 흡광도가 항산화제에 의해 억제되어 특유의 청록색으로 탈색되는 현상을 이용한 방법이다. ABTS+ 라디칼 소거능 측정은 Roberta 등(2011)의 방법으로 측정하였다. 7mM ABTS 용액과 2.45mM potassium persulfate 용액을 빛을 차단한 상태로 16시간 동안 상온에서 반응시켜 ABTS 양이온을 형성시킨 후 흡광도 값이 0.70±0.02가 되도록 무수에탄올을 사용하여 조절하여 시료 10μL에 첨가하고 6분 동안 반응시켜 microplate reader(Molecular Devices)를 사용하여 750nm에서 흡광도의 값을 측정하였다. 항산화 활성은 시료를 녹인 용매인 Ascorbic acid(Ac)를 대조군으로 사용하여 대조군에 대한 라디칼 소거능을 백분율로 나타내었다.The ABTS + measurement method is the method of Kim et al. (2013), which utilizes the phenomenon that the absorbance of the cationic radical of ABTS is suppressed by an antioxidant and thus discolors to a characteristic blue-green color. ABTS + radical scavenging activity was measured by the method of Roberta et al. (2011). 7mM ABTS solution and 2.45mM potassium persulfate solution were reacted at room temperature for 16 hours in a state of blocking light to form ABTS cations. After reacting for minutes, the absorbance value was measured at 750 nm using a microplate reader (Molecular Devices). As for the antioxidant activity, ascorbic acid (Ac), a solvent in which the sample was dissolved, was used as a control, and the radical scavenging ability of the control was expressed as a percentage.
토종닭 사료 내 개느삼의 수준별 첨가가 혈청과 다리육에서의 ABTS+ 소거능 분석 결과는 도 3과 같다. 혈청의 경우, 개느삼 첨가에 따라 ABTS+ 소거능이 유의적으로 증가하였다(p<0.05). 다리육의 경우, 개느삼을 10ppm 첨가 시 유의적인 차이는 나타나지 않았지만, 20, 30ppm 첨가 시 ABTS+ 소거능이 유의적으로 증가하였다(p<0.05).The results of analysis of ABTS + scavenging ability in serum and leg meat added by level of ginseng in native chicken feed are shown in FIG. 3 . In the case of serum, ABTS + scavenging ability was significantly increased according to the addition of ginseng ( p <0.05). In the case of leg meat, there was no significant difference when 10 ppm of ginseng was added, but ABTS + scavenging ability was significantly increased when 20 and 30 ppm was added ( p <0.05).
조 등(2009)의 실험에서 육계에게 약용식물 추출물을 0.3% 또는 1.0% 급여 시 다리육에서 저장기간에 따른 ABTS+ 소거능이 증가하는 결과와 본 실험의 결과가 일치하였다.In the experiment of Cho et al. (2009), when 0.3% or 1.0% of medicinal plant extracts were fed to broilers, the results of this experiment were consistent with the results of increasing ABTS + scavenging ability in leg meat according to the storage period.
실험예 6. 통계분석Experimental Example 6. Statistical Analysis
본 시험에서 얻은 자료에 대한 통계분석은 SAS(2012)를 이용하여 일원분류 분산분석(one-way ANOVA)을 하였고, 평균간 비교는 Tukey의 다중검정을 실시하였다.One-way ANOVA was performed for statistical analysis of the data obtained in this study using SAS (2012), and Tukey's multiple test was performed for comparison between means.
이제까지 본 발명에 대하여 그 바람직한 실시 예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시 예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구 범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.So far, the present invention has been looked at with respect to preferred embodiments thereof. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than in the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.
Claims (10)
상기 추출물은 물 또는 유기용매 추출법으로 추출되는 것을 특징으로 하는, 가금류 품질 개선용 기능성 사료 조성물.According to claim 1,
The extract is a functional feed composition for improving poultry quality, characterized in that extracted by water or organic solvent extraction method.
상기 유기용매 추출법은 60 내지 80% 에탄올을 이용한 것을 특징으로 하는, 가금류 품질 개선용 기능성 사료 조성물.3. The method of claim 2,
The organic solvent extraction method is a functional feed composition for improving poultry quality, characterized in that 60 to 80% ethanol is used.
상기 조성물은 가금류의 복강 내 지방을 감소시키는 것을 특징으로 하는, 가금류 품질 개선용 기능성 사료 조성물.According to claim 1,
The composition is a functional feed composition for improving poultry quality, characterized in that it reduces the fat in the abdominal cavity of poultry.
상기 조성물은 가금류의 비장, F낭 및 갑상선의 무게를 증가시켜 면역 기능을 향상시키는 것을 특징으로 하는, 가금류 품질 개선용 기능성 사료 조성물.According to claim 1,
The composition is a functional feed composition for improving poultry quality, characterized in that it improves immune function by increasing the weight of the poultry's spleen, F sac and thyroid gland.
상기 조성물은 가금류의 보수력, 가열감량, 전단력 및 pH를 개선시키는 것을 특징으로 하는, 가금류 품질 개선용 기능성 사료 조성물.According to claim 1,
The composition is a functional feed composition for improving poultry quality, characterized in that it improves the water holding capacity, heating loss, shear force and pH of poultry.
상기 조성물은 가금류 혈청의 MDA, SOD 및 TA를 증가시키는 것을 특징으로 하는, 가금류 품질 개선용 기능성 사료 조성물.According to claim 1,
The composition is a functional feed composition for improving poultry quality, characterized in that it increases the MDA, SOD and TA of poultry serum.
상기 조성물은 가금류 다리육의 SOD, TA 및 CAT를 증가시키는 것을 특징으로 하는, 가금류 품질 개선용 기능성 사료 조성물.According to claim 1,
The composition is a functional feed composition for improving poultry quality, characterized in that it increases the SOD, TA and CAT of poultry leg meat.
상기 조성물은 가금류의 혈청 및 다리육의 ABTS+ 소거능을 증가시키는 것을 특징으로 하는, 가금류 품질 개선용 기능성 사료 조성물.According to claim 1,
The composition is a functional feed composition for improving poultry quality, characterized in that it increases the ABTS + scavenging ability of poultry serum and leg meat.
상기 사료 첨가제는 가금류의 복강 내 지방을 감소시키는 것을 특징으로 하며,
상기 사료 첨가제는 가금류의 비장, F낭 및 갑상선의 무게를 증가시켜 면역 기능을 향상시키는 것을 특징으로 하고,
상기 사료 첨가제는 가금류의 보수력, 가열감량, 전단력 및 pH를 개선시키는 것을 특징으로 하며,
상기 사료 첨가제는 가금류 혈청의 MDA, SOD 및 TA를 증가시키는 것을 특징으로 하고,
상기 사료 첨가제는 가금류 다리육의 SOD, TA 및 CAT를 증가시키는 것을 특징으로 하며,
상기 사료 첨가제는 가금류의 혈청 및 다리육의 ABTS+ 소거능을 증가시키는 것을 특징으로 하는, 가금류 품질 개선용 기능성 사료 첨가제.
In the functional feed additive for improving the quality of poultry comprising an extract of ginseng extract as an active ingredient, the extract is extracted by water or organic solvent extraction method,
The feed additive is characterized in that it reduces the fat in the abdominal cavity of poultry,
The feed additive is characterized in that it improves immune function by increasing the weight of the spleen, F sac and thyroid gland of poultry,
The feed additive is characterized in that it improves the water holding capacity, heating loss, shear force and pH of poultry,
The feed additive is characterized in that it increases the MDA, SOD and TA of poultry serum,
The feed additive is characterized in that it increases the SOD, TA and CAT of poultry leg meat,
The feed additive is a functional feed additive for improving poultry quality, characterized in that it increases the ABTS + scavenging ability of poultry serum and leg meat.
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KR20240053392A (en) | 2022-10-17 | 2024-04-24 | 강원대학교산학협력단 | Composition for feeding poultry to improve the productivity and egg quality of laying hens |
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KR20090018048A (en) | 2006-05-02 | 2009-02-19 | 니혼 이타가라스 가부시키가이샤 | Glass composition and glass spacer using the same |
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KR20090018048A (en) | 2006-05-02 | 2009-02-19 | 니혼 이타가라스 가부시키가이샤 | Glass composition and glass spacer using the same |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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KR20240053392A (en) | 2022-10-17 | 2024-04-24 | 강원대학교산학협력단 | Composition for feeding poultry to improve the productivity and egg quality of laying hens |
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