KR20220096212A - Apparatus for identifying species of microbe in real-time and identifying method using the same - Google Patents

Abstract

According to the present invention, provided are an apparatus for identifying species of a microbe in real time and a method for identifying species of a microbe using the same, wherein the apparatus may include: a chamber casing supporting a heterogeneous chamber in which a part of a circular mirror and a part of an elliptical mirror are interconnected with each other; a sample entering and exiting unit which is coupled to the heterogeneous chamber, and in which an empty plate and one of sample plates enter or exit, wherein the sample plates accommodate an original sample to which salvia extracted from a subject is applied, an antigen sample in which a specific antibody corresponding to a specific antigen is coupled to the original sample, or a PCR sample obtained by processing the original sample with a PCR technique, respectively; a UV light source unit connected to one side of the chamber cashing to transmit an input light source to the heterogeneous chamber; and a fluorescence light receiving unit A and fluorescence light receiving unit B detecting fluorescence light emitted from the heterogeneous chamber. The apparatus for identifying species of a microbe in real time and the method for identifying species of a microbe using the same provided by the present invention can quickly identify species of a specific microbe by producing the antigen sample or the PCR sample from the salvia of the subject, exposing the antigen sample or the PCR sample to the input light source, and collecting scattered and refracted fluorescence light wavelengths, and can enable light detection based on the input light source with a short wavelength to be detected with high-resolution precision.

Description

Real-time microbial species identification device and microbial species identification method using the same

The present invention relates to a real-time microbial species identification apparatus and a microbial species identification method using the same, and more particularly, to a real-time microbial species identification capable of species identification with a precise resolution of 300 nm using a fluorescence photodetection technique of a specific microorganism It relates to a device and a microbial species identification method using the same.

In recent years, new infectious diseases have been spreading almost every other year around the world. For example, virus-related diseases, including allogeneic infectious diseases such as foot-and-mouth disease, African swine fever, and avian influenza, and heterogeneous infectious diseases such as cold virus, severe acute respiratory syndrome, and Middle East respiratory syndrome, are representative.

In particular, the COVID-19 situation, which started in China in 2019 and has become a global catastrophe, is currently in progress and is threatening the health of all mankind. Accordingly, rapid and rapid diagnosis and accurate identification technology for these viruses and microorganisms are very urgent.

In general, as a method for detecting microorganisms or checking for infection, a sample is obtained from the upper and lower respiratory tract of the subject, sputum or saliva from the bronchus is collected, and the PCR technique (polymerase chain reaction or reverse transcription polymerase chain reaction, etc.) to detect and discriminate viruses by increasing the sensitivity. Although this detection method has a reliable high sensitivity, it requires a replication time to amplify the virus several tens to hundreds of times, and requires a pretreatment process such as centrifugation, so it takes a long time for identification.

Alternatively, a rapid antigen diagnosis method has also been disclosed. Antibodies corresponding to specific microorganisms are prepared on a sample plate, and when an antigen is input, it is a technique to determine whether or not a virus is infected by checking whether or not it reacts. An example may be a test method that detects influenza virus nucleoprotein as an antigen. This rapid antigen diagnosis method has the advantage of being a simple test method and being able to know the test result within a short period of time (usually within 30 minutes), but it is less sensitive than RT-PCR and cannot accurately determine the type of microorganism. It is also true that there are clear limitations.

Meanwhile, in Korean Patent Registration No. 10-1878094, the present applicant has disclosed an apparatus for rapidly and precisely detecting microorganisms in the air using a fluorescence photodetection technique. This technology can monitor atmospheric conditions in real time by irradiating light sources to microorganisms in the air to detect scattered and refracted fluorescence.

Therefore, as a method for diagnosing and discriminating microorganisms including viruses, which are currently a worldwide issue, sampling the saliva of a subject and grafting the fluorescence photodetection technique of the present applicant to quickly and accurately identify the species of a specific microorganism There is a need for technological development.

The present invention has been proposed to solve the above problems, and by producing an antigen sample or PCR sample from the saliva of a test subject and exposing it to an input light source to receive the scattered and refracted fluorescence wavelengths, the species of a specific microorganism can be identified quickly Provided are a real-time microbial species identification device and a microbial species identification method using the same, which can be possible and can be detected with high-resolution precision by enabling photodetection based on a short-wavelength input light source.

Real-time microbial species identification apparatus according to the present invention for achieving the above object, a chamber casing for supporting a heterogeneous chamber in which a part of a circular diameter and a part of an ellipsoid are coupled to each other; An empty plate coupled to the heterogeneous chamber and coated with saliva extracted from the test subject, an antigenic sample in which a specific antibody corresponding to a specific antigen is bound to the round sample, or a PCR sample processed by PCR on the round sample a sample input/output unit to which any one of the sample sample plates among the received sample sample plates is entered and exited; a UV light source unit connected to one side of the chamber casing to transmit an input light source to the heterogeneous chamber; and a fluorescence light receiving unit A and a fluorescence light receiving unit B for detecting fluorescence light emitted from the heterogeneous chamber.

The fluorescence light receiving unit A may detect the total amount of microorganisms in the circular sample, and the fluorescent light receiving unit B may detect a specific wavelength band of the antigen sample or PCR sample.

a light receiving block provided between the fluorescence light receiving unit A and the fluorescence light receiving unit B and including a beam splitter for separating the fluorescence light receiving unit according to the density of a wavelength band; and a recovery dump unit provided on the opposite side of the UV light source unit with the heterogeneous chamber interposed therebetween.

The sample input/output unit may include: a plate support unit on which the sample specimen plate is supported; and a plate drive coupled to the plate support to finely align the plate support.

The light receiving block further includes a diffuse reflection reducing unit for extracting the input light source, the diffuse reflection reducing unit, the light source splitter for reflecting the input light source; and a light source light receiving unit configured to receive the input light source.

A center of the circular mirror may be a first focal point of the ellipsoid.

The input light source of the UV light source unit is a UV light source, and the wavelength band of the input light source may be 275 nm to 405 nm.

In the species identification method using the real-time microbial species identification device according to the present invention for achieving the above object, the UV light source unit transmits the corresponding light source and detects the emitted fluorescence light, but transmittance of the sample plate and an empty plate inspection step of checking device errors; a circular specimen testing step of examining a circular specimen coated with saliva extracted from a subject on the sample specimen plate; And it may include a processing sample testing step of testing the antigen sample in which a specific antibody corresponding to a specific antigen is bound to the original sample, or a PCR sample processed by the PCR technique on the original sample.

In the circular sample testing step or the processed sample testing step, the total amount of microorganisms in the circular sample may be detected, or a specific wavelength band of the antigen sample or PCR sample may be detected.

The input light source of the UV light source unit is a UV light source, and the wavelength band of the input light source may be 275 nm to 405 nm.

The real-time microbial species identification device and the microbial species identification method using the same according to the present invention produce an antigen sample or PCR specimen from the saliva of a subject and expose it to an input light source to receive scattered and refracted fluorescence wavelengths to determine the species of a specific microorganism This can be done quickly, and photodetection based on a short-wavelength input light source becomes possible, so that it can be detected with high resolution and precision.

1 is a front view of a real-time microbial species identification device according to an embodiment of the present invention.
Figure 2 is a side view of a real-time microbial species identification device according to an embodiment of the present invention.
FIG. 3 is a view schematically illustrating a heterogeneous chamber in FIG. 1 .
4 is a conceptual diagram schematically illustrating a real-time microbial species identification device according to an embodiment of the present invention.
5 is a diagram illustrating an example of a sample plate in the real-time microbial species discrimination apparatus according to an embodiment of the present invention.
6 is a diagram schematically illustrating a state in which a prototype sample, an antigen sample, and a PCR sample are applied to a sample plate in the real-time microbial species discrimination apparatus according to an embodiment of the present invention.
7 is a block diagram of a real-time microbial species identification apparatus according to an embodiment of the present invention.
8 is a conceptual diagram of a real-time microbial species identification device according to another embodiment of the present invention.
9 is a flowchart of a species identification method using a real-time microbial species identification apparatus according to an embodiment of the present invention.
10 is a view showing the results of species identification of a specific microorganism by the species identification method using the real-time microbial species identification apparatus according to an embodiment of the present invention.

Hereinafter, an embodiment of a real-time microbial species identification apparatus and a microbial species identification method using the same according to the present invention will be described in detail with reference to the accompanying drawings. Like reference numerals in each figure indicate like elements.

1 is a front view of a real-time microbial species identification apparatus according to an embodiment of the present invention, FIG. 2 is a side view of a real-time microbial species identification apparatus according to an embodiment of the present invention, and FIG. 3 schematically shows the heterogeneous chamber in FIG. 4 is a conceptual diagram schematically illustrating a real-time microbial species identification apparatus according to an embodiment of the present invention, and FIG. 5 shows an example of a sample plate in the real-time microbial species identification apparatus according to an embodiment of the present invention 6 is a diagram schematically illustrating a state in which a circular sample, an antigen sample, and a PCR sample are applied to a sample plate in the real-time microbial species discrimination apparatus according to an embodiment of the present invention, and FIG. 7 is a diagram of the present invention It is a block diagram of a real-time microbial species identification device according to an embodiment.

A real-time microbial species identification apparatus according to an embodiment of the present invention is a chamber casing that supports the heterogeneous chamber 100 in which a part of the oval mirror 102 and a part of the ellipsoid 101 are coupled to each other with reference to FIGS. 1 to 7 . (110); Bind to the heterogeneous chamber 100, an empty plate, a circular specimen coated with saliva extracted from the subject, an antigenic specimen in which a specific antibody corresponding to a specific antigen is bound to the circular specimen, or the circular specimen processed by PCR a sample input/output unit 200 into which a sample sample plate 210 in which any one of the PCR samples is accommodated; a UV light source unit 300 connected to one side of the chamber casing 110 to transmit an input light source to the heterogeneous chamber 100; a fluorescence light receiving unit A 500 and a fluorescence light receiving unit B 510 for detecting fluorescence light emitted from the heterogeneous chamber 100; a light receiving block 400 provided between the fluorescent light receiving unit A 500 and the fluorescent light receiving unit B 510 and including a beam splitter 420 that separates fluorescent wavelengths according to the density of wavelength bands; and a recovery dump unit 500 provided on the opposite side of the UV light source unit 300 with the heterogeneous chamber 100 interposed therebetween.

As shown in FIGS. 1 to 2 and 4 , the real-time microbial species discrimination apparatus according to an embodiment of the present invention includes a heterogeneous chamber 100 in which a sample sample plate 210 is accommodated, and a heterogeneous chamber 100 . It may be composed of a UV light source unit 300 for irradiating an input light source, a fluorescence light receiving unit A 500 and a fluorescence light receiving unit B 510 for detecting fluorescence refracted and reflected by saliva.

The heterogeneous chamber 100 may be mainly provided inside the chamber casing 110 as shown in FIGS. 1 and 2 .

Referring to FIG. 3 , the heterogeneous chamber 100 may be configured such that a part of the circular mirror 102 and a part of the ellipsoid 101 are coupled to each other. That is, the right side of the heterogeneous chamber 100 may be provided as an ellipsoidal mirror 101 having two focal points F1 and F2, so that the input light source incident from the UV light source unit 300 is substantially an ellipsoid mirror ( 101) may converge to the first focus F1.

In addition, the left part of the heterogeneous chamber 100 is provided with a circular mirror 102 , and the ellipsoid 101 and the circular mirror 102 may be configured to be coupled to each other. Here, the center of the ellipsoidal mirror 102 may be the first focus of the ellipsoidal mirror 101 .

Accordingly, the input light source is irradiated to the saliva (liquid) of the sample plate 210, and the collided input light source is scattered and refracted. and may be emitted to the first light outlet 104 of the heterogeneous chamber 100 toward the second focus F2 of the ellipsoid 101 through the opening of the circular mirror 102 .

The UV light source unit 300 may be connected to the upper portion of the chamber casing 110 . The UV light source unit 300 may transmit an input light source to the heterogeneous chamber 100 . The input light source of the UV light source unit 300 is a UV light source, and may be made of an LED or LD device. In addition, the wavelength band of the input light source may be 275 nm to 405 nm, and preferably, it is possible to minimize the fluorescence of a non-living object (inanimate object) by emitting light in the UV region of 365 nm.

A first incident lens 310 and a second incident lens 320 are respectively provided in the UV light source unit 300 to collect the input light source irradiated into the heterogeneous chamber 100 to the first focus F1 of the heterogeneous chamber 100 . make it possible

The sample input/output unit 200 is a part for allowing the sample sample plate 210 to enter and exit the heterogeneous chamber 100 . As shown in FIG. 2 , a through hole 111 that enters the first focal point F1 is provided on one side of the heterogeneous chamber 100 , and the sample specimen plate 210 is inserted and exited using the through hole 111 . can be provided.

The sample input/output unit 200 includes a plate support unit 220 on which the sample specimen plate 210 is supported; and a plate drive 240 coupled to the plate support 220 to finely align the plate support 220 .

1, 2 and 5, the sample sample plate 210 is provided in a plate-like plate type, and a saliva receiving part 211 that is formed to be rounded inwardly on one side is provided, and a saliva receiving part ( 211) A saliva receiving hole 212 may be formed in the center. Since the saliva is liquid, it does not flow through the saliva receiving hole 212 even if a through hole is drilled.

In addition, the bottom portion of the sample plate 210 having the saliva receiving hole 212 may be configured to correspond to the position of F1, which is one focus of the heterogeneous chamber 100 . Accordingly, the saliva of the examinee can be more easily and conveniently aligned to the F1 focus, and discrimination precision can be improved.

The sample plate 210 may be made of a material that transmits an input light source and a fluorescence wavelength, and may be made of a material having a transmittance of 95% or more, and has a high transmittance when classifying a fluorescence light-receiving unit A and a fluorescent light-receiving unit B. noise generation can be minimized.

On the other hand, the sample sample plate 210 may be configured with only the saliva receiving part 211 without the saliva receiving hole 212, or a cover plate (not shown) covering the upper surface of the saliva receiving part 211, unlike the above-described configuration. city) may be additionally configured. In this case, the focus of the heterogeneous chamber 100 will also need to be changed to the center of the position where the saliva is disposed.

Referring to FIG. 6 , a state in which a circular sample, an antigen sample, and a PCR sample are applied to the sample sample plate 210 is shown. An empty plate in which no specimen is accommodated or a circular specimen coated with saliva extracted from a subject as shown in FIG. 6(a) may be prepared.

Alternatively, as shown in (b) of FIG. 6, an antigenic sample in which a specific antibody corresponding to a specific antigen is bound to the circular sample, or a PCR sample processed by PCR technique in the circular sample as in (c). can

Here, the specific antibody may be, for example, a coronavirus, and may be changed to an antibody configuration corresponding to a specific antigen to be discriminated. A method of discriminating an antigenic sample using such an antigenic sample may be similar to the rapid antigen test, and in this embodiment, it may be possible to discriminate within minutes using the fluorescence photodetection technique.

In addition, in the case of a PCR sample, real-time PCR is applied to the prototype antibody from the outside for a maximum of several minutes, and the liquid phase is injected, which is relatively quick compared to the test time that is repeated several dozen times or more and the PCR is performed at least several hours. Species discrimination may be possible. This is because, by performing the photodetection technique, the discrimination resolution is higher (up to 300 nm level) compared to the existing inspection method, so that the discrimination can be performed more quickly than in the prior art.

The plate support part 220 is a part on which the sample sample plate 210 is supported, and a seating step 230 on which the sample sample plate 210 is seated and accommodated may be provided.

In addition, mainly referring to FIGS. 2 and 7 , a plate drive 240 for finely aligning the plate support part 220 may be provided in the plate support part 220 . The plate drive 240 may be configured to make the plate support part 220 finely moved (um/step) or to be oscillated as much as a preset clearance (Hz/sec).

In more detail, the amount of fluorescence detected may be inaccurate or unstable because saliva is received in the sample plate 210 in a biased manner in the saliva receiving unit 211 . In this case, the plate drive 240 is controlled to be driven through the MCU 700 so that the saliva can be correctly aligned with the saliva receiving part 211 , and one focus of the heterogeneous chamber 100 at the center of the saliva receiving hole 212 . (F1) can be aligned, so that the fluorescence detection signal can be stabilized and the species discrimination precision can be improved.

When viewed with reference to FIG. 1 , the light receiving block 400 may be provided on the left side of the chamber casing 110 . The light receiving block 400 is mainly provided between the light receiving lens 410 and the fluorescent light receiving unit A 500 and the fluorescent light receiving unit B 510 as shown in FIG. 1 to separate the fluorescence wavelengths according to the density of the wavelength band. A splitter 420 may be included.

The output light source emitted to the second focal point F2 may pass through the second light exit hole 103 to ensure straightness by the light receiving lens 410 .

The beam splitter 420 is provided between the fluorescence light receiving unit A 500 and the fluorescent light receiving unit B 510 to separate the fluorescence wavelengths according to the density of the wavelength bands.

The beam splitter 420 changes the wavelength of the fluorescence from among the output light sources that are output differently from the wavelength of the input light source by 90 degrees and sends it to the fluorescence light receiving unit A 500 , and the wavelength among the output light sources that are different from the wavelength of the input light source This high-density specific wavelength band passes through the beam splitter 420 and serves to transmit it to the fluorescence light receiving unit B 510 .

Each of the fluorescence light receiving unit A 500 and the fluorescent light receiving unit B 510 detects the presence or absence of microorganisms and the amount thereof from the light transmitted through the first and second condensing lenses 501 and 511 .

That is, the fluorescence light receiving unit A 500 may count the target number of microorganisms by detecting the presence and total amount of microorganisms in the circular specimen, antigen specimen, or PCR specimen. In addition, the fluorescence light receiving unit B 510 may detect the density of a specific wavelength band of the circular sample, the antigen sample or the PCR sample, or a peak value greater than or equal to a preset value.

The fluorescence light receiving unit A 500 and the fluorescence light receiving unit B 510 respectively receive the fluorescence emitted to the outside of the heterogeneous chamber 100, generate a detection signal for the received light, and transmit it to a signal processing unit (not shown). On the other hand, since autofluorescence by microorganisms is a very fine signal compared to scattered light, the fluorescence light receiving unit A 500 and the fluorescence light receiving unit B 510 may be implemented as a photo multiplier tube (PMT), and detection The resulting fluorescence may include information on the presence or absence of microorganisms and their amount.

The recovery dump part 500 is a part for recovering the diffusely reflected and transmitted light source among the chief rays incident into the heterogeneous chamber 100 without passing through the first focus through the second light exit hole 103 .

That is, the recovery dump unit 500 serves to stop the light source that does not collide with the particles of the measurement sample among the input light sources incident into the heterogeneous chamber 100 , so that ambient light other than the scattered light within the heterogeneous chamber 100 is Inflow into the first light exit hole 170 can be minimized.

The recovery dump unit 500 may include a conical dump member 510 , and the dump member 510 may be disposed such that the vertex points toward the path of the emitted light, so that the light impinging on the conical member 410 is direct. reflection can be prevented. In addition, a member that absorbs light, such as a sponge, may be disposed on the inner wall of the recovery dump unit 500 , and may be implemented in a concave-convex structure.

With such a configuration, antigen samples or PCR samples are produced from the saliva of the subject and exposed to an input light source to receive scattered and refracted fluorescence wavelengths, so that the species of a specific microorganism can be identified quickly, and based on a short wavelength input light source The photodetection can be detected with high resolution and precision.

8 is a conceptual diagram of a real-time microbial species identification device according to another embodiment of the present invention.

The real-time microbial species identification apparatus according to this embodiment is different from the real-time microorganism species identification apparatus according to the first embodiment in configuration. Hereinafter, in order to avoid duplication of description, only a configuration different from the configuration of the above-described first embodiment will be described.

As shown in FIG. 8 , the real-time microbial species discrimination apparatus according to the present embodiment may further include a diffuse reflection reducing unit 600 provided in the light receiving block 400 to extract the input light source.

The diffuse reflection reducing unit 600 includes a light source splitter 620 that reflects the input light source; and a light source light receiving unit 610 for receiving the input light source.

Here, the light source splitter 620 may have a wavelength band of 275 nm to 405 nm in the present embodiment of the input light source, and may preferably reflect light in a UV region of 365 nm.

And the light source light receiving unit 610 is a portion for receiving the reflected input light source.

Accordingly, the diffuse reflection reducing unit 600 removes the same wavelength band as the input light source, so that noise received by the actual fluorescence light receiving unit A 500 and the fluorescence light receiving unit B 510 can be significantly reduced, resulting in a signal-to-noise ratio (SNR). , signal-to-noise ratio) can be improved.

9 is a flowchart of a species identification method using a real-time microbial species identification apparatus according to an embodiment of the present invention, and FIG. 10 is a specific microorganism in a species identification method using a real-time microbial species identification apparatus according to an embodiment of the present invention It is a diagram showing the results of species discrimination.

Hereinafter, a species identification method using the species identification apparatus according to the present embodiment will be described in detail with reference to FIGS. 1 to 10 .

The species identification method using the real-time microbial species identification device according to the present invention transmits the corresponding light source of the UV light source unit 300 and detects the emitted fluorescence light, but transmittance and device of the sample specimen plate 210 Empty plate inspection step (S100) to check for errors; a circular specimen inspection step (S200) of inspecting a circular specimen coated with saliva extracted from a subject on the sample specimen plate 210; And it may include a processed sample testing step (S300) of testing the antigen sample in which a specific antibody corresponding to a specific antigen is bound to the original sample, or a PCR sample processed by the PCR technique on the original sample.

First, an empty plate inspection step may be performed (S100).

The sample sample plate 210 in a state in which no sample is accommodated is accommodated in the plate support part 220 , and the sample input/output part 200 is driven to be put into the heterogeneous chamber 100 .

Information on whether the input light source is irradiated through the UV light source unit 300 and the fluorescence received by the fluorescence light receiving unit A 500 and the fluorescence light receiving unit B 510 is received, that is, information on the presence or absence of microorganisms and the amount thereof is checked.

In the empty plate inspection step, it is possible to check transmittance, device error, and contamination of the sample specimen plate 210 .

Next, the sample input/output unit 200 is driven to remove the sample sample plate 210 .

Next, the circular specimen inspection step is performed (S200).

The circular specimen refers to saliva extracted from the subject as shown in (a) of FIG. 6 , and is received by the sample specimen plate 210 , and then is injected into the heterogeneous chamber 100 by driving the sample input and output unit 200 .

Then, the input light source is irradiated through the UV light source unit 300 and the fluorescence light receiving unit A 500 and the fluorescent light receiving unit B 510 receive fluorescence, that is, the presence or absence of microorganisms and information on the amount thereof. .

As shown in FIG. 10 , in the case of a normal microorganism, fluorescence photodetection is generated, and a fluorescence wavelength band is generated while being scattered and refracted from an input light source, and the fluorescence light receiving units A 500 and P3 may receive the light. However, since a fluorescence reaction of a specific microorganism does not occur in the fluorescence light receiving units B 510 and P2, light is not received at all.

Through this, it is possible to count the target number of microorganisms by detecting the presence and total amount of general microorganisms.

After removing the sample sample plate 210 by driving the sample input/output unit 200, the processing sample inspection step is performed (S300).

First, an antigen sample or PCR sample is produced and accommodated in the sample sample plate 210 as shown in FIGS. 6 (b) and (c). That is, when a specific microorganism (eg, corona virus, etc.) is contained in saliva, an antigen sample containing an antibody corresponding to the specific microorganism is produced, or a PCR sample amplified several times by PCR technique is introduced.

Then, the sample input/output unit 200 is driven to insert the sample sample plate 210 into the heterogeneous chamber 100 .

In the circular sample testing step or the processed sample testing step, the total amount of microorganisms in the circular sample may be detected or a specific wavelength band of the antigen sample may be detected.

That is, the fluorescent light receiving unit A 500 can count the target number of microorganisms by detecting the presence and total amount of microorganisms in the antigen sample or PCR sample, and at the same time, the fluorescent light receiving unit B 510 is the antigen sample However, it is possible to detect the density and concentration of a specific wavelength band of a PCR sample.

Referring to FIG. 10 , a specific microorganism is also light-received by the fluorescence light-receiving units A 500 (P3), and is also received by the fluorescence light-receiving units B 510 (P2).

Here, when light is also received by the fluorescence light receiving unit A 500 (P3), it means that the antigen sample is photodetected by the fluorescence reaction of a specific microorganism like a general microorganism, and the existence of the microorganism may be confirmed. However, in this case, the presence of a specific microorganism is not determined only by the received signal received by the fluorescence light receiving unit A 500 (P3).

When light is received by the fluorescence light receiving unit B 510 (P2), it can be determined that the concentration and density of a specific fluorescence wavelength band are increased by being reacted by the antigen sample and amplified by the PCR sample. Accordingly, it may be possible to discriminate a specific species from the saliva of the subject.

A method of discriminating an antigenic sample using such an antigenic sample may be similar to the rapid antigen test, and in this embodiment, it may be possible to discriminate within minutes using the fluorescence photodetection technique.

In addition, in the case of a PCR sample, real-time PCR is applied to the prototype antibody from the outside for up to several minutes, and the liquid phase is added. Species discrimination may be possible.

By going through these steps, the antigen sample or PCR sample is produced from the saliva of the subject and exposed to the input light source to receive the scattered and refracted fluorescence wavelength, so that the species of a specific microorganism can be identified quickly, and the short wavelength input light source Based on photodetection, it can be detected with high resolution and precision.

As mentioned above, although the present invention has been described in detail using preferred embodiments, the scope of the present invention is not limited to specific embodiments and should be construed according to the appended claims. In addition, those skilled in the art should understand that many modifications and variations are possible without departing from the scope of the present invention.

100: heterogeneous chamber 101: oval mirror
102: Jeongwon-gyeong 103: 2nd light exit
104: first light output port 110: chamber casing
200: sample input/output unit 210: sample sample plate
211: saliva receiving unit 212: saliva receiving hole
220: plate support 230: seating step
240: plate drive 300: UV light source unit
310: first incident lens 320: second incident lens
400: light receiving block 410: light receiving lens
420: beam splitter 500: fluorescent light receiving unit A
501: first condensing lens 510: fluorescent light receiving unit B
511: second condensing lens 530: recovery dump unit
540: dump member 600: diffuse reflection reducing part
610: light source receiving unit 620: light source splitter

Claims (10)

a chamber casing for supporting a heterogeneous chamber in which a portion of a circular mirror and a portion of an ellipsoid are coupled to each other;
Among the circular specimens bound to the heterogeneous chamber and coated with empty plates, saliva extracted from the test subject, antigenic specimens in which a specific antibody corresponding to a specific antigen is bound to the circular specimens, or PCR samples processed by PCR on the circular specimens a sample input/output unit in which any one of the accommodated sample specimen plates is input/output;
a UV light source unit connected to one side of the chamber casing to transmit an input light source to the heterogeneous chamber; and
Real-time microbial species discrimination device comprising a fluorescence light receiving unit A and a fluorescence light receiving unit B for detecting fluorescence light emitted from the heterogeneous chamber. According to claim 1,
The fluorescence light receiving unit A detects the total amount of microorganisms in the circular specimen,
The fluorescence light receiving unit B is a real-time microbial species identification device, characterized in that for detecting the density (density) of a specific wavelength band of the antigen sample. 3. The method of claim 2,
a light receiving block provided between the fluorescent light receiving unit A and the fluorescent light receiving unit B and including a beam splitter configured to separate fluorescent wavelengths according to the density of wavelength bands; and
Real-time microbial species identification device, characterized in that it further comprises a recovery dump provided on the opposite side of the UV light source with the heterogeneous chamber interposed therebetween. According to claim 1,
The sample input and output unit,
a plate support part on which the sample plate is supported; and
A real-time microbial species identification device coupled to the plate support and comprising a plate drive for finely aligning the plate support. 4. The method of claim 3,
Further comprising a diffuse reflection reducing unit provided on the light receiving block for extracting the input light source,
The diffuse reflection reducing unit,
a light source splitter reflecting the input light source; and
Real-time microbial species identification device comprising a light source light receiving unit for receiving the input light source. According to claim 1,
The center of the garden mirror is a real-time microbial species identification device, characterized in that the first focus of the ellipsoid. According to claim 1,
The input light source of the UV light source is a UV light source,
The wavelength band of the input light source is a real-time microbial species identification device, characterized in that 275 nm ~ 405 nm. An empty plate inspection step of transmitting the corresponding light source from the UV light source and detecting the emitted fluorescence light, but checking the transmittance and device errors of the sample plate;
a circular specimen testing step of examining a circular specimen coated with saliva extracted from a subject on the sample specimen plate; and
Real-time microbial species identification method comprising the step of testing an antigen sample in which a specific antibody corresponding to a specific antigen is bound to the original sample or a PCR sample processed by PCR on the original sample. 9. The method of claim 8,
The real-time microbial species identification method, characterized in that the circular sample testing step or the processed sample testing step detects the total amount of microorganisms in the circular sample or a specific wavelength band of the antigen sample. 9. The method of claim 8,
The input light source of the UV light source is a UV light source,
The wavelength band of the input light source is a real-time microbial species identification method, characterized in that 275 nm ~ 405 nm.

Images