KR20220095932A - High hlb lecithin manufacture system and high hlb lecithin manufactured by using the same - Google Patents

Abstract

The present invention relates to a high hydrophilic-lipophilic balance (HLB) lecithin manufacturing system, and high HLB lecithin manufactured by using the same and, more specifically, to a high HLB lecithin manufacturing system comprising: a reaction device that performs transesterification by adding a biocatalyst to a reaction solution containing standard lecithin and fatty acids; and a degreasing device for removing lipids from a transesterification reaction product, and to a high HLB lecithin manufactured by using the same.

Description

High HLB lecithin manufacturing system and high HLB lecithin manufactured using the same

The present invention relates to a high HLB lecithin production system and high HLB lecithin prepared using the same, and it is possible to prepare lecithin with improved solubility and formulation stability by controlling the HLB (hydrophilic-lipophilic balance) of lecithin. It relates to a system for producing high HLB lecithin and to high HLB lecithin produced using the same.

Lecithin is a purified product obtained by removing fatty acids and triglycerides from oil obtained from soybeans, sunflower seeds, egg yolks, etc. to be. Phospholipids are glycerol alcohols, in which the hydroxyl groups on carbon 1 and 2 are ester-bonded with fatty acids, and phosphoric acid, ethanolamine phosphate, phosphocholine, etc. It is a typical hydrophilic head and hydrophobic tail type surfactant. Phospholipids found in nature are characterized by forming a lamellar structure, a layered repeating structure composed of a phospholipid bilayer in an aqueous solution phase topologically in which two fatty acids are arranged side by side. Because of these properties, lecithin is used in the processing of liposomes, which are carriers of various hydrophilic materials. Considering the market situation where lecithin is mainly used for products based on water-soluble solvents such as pharmaceuticals, foods, cosmetics, and water-soluble paints, the technology to control the solubility and emulsification performance of lecithin increases the total amount of liposomes in the formulation. It is important to increase the concentration of the component as a carrier or to increase the amount of hydrophobic component that can be emulsified as it is helpful.

On the other hand, the type and amount of material in which the surfactant can be emulsified is affected by the hydrophilic-lipophilic balance (HLB). The stronger the hydrophilicity of the material used for emulsification, the more advantageous the surfactant with a high HLB value. The HLB value of lecithin is between 4 and 8, and the higher the degree of purification, the closer to 8. Lecithin derivatives with an HLB value of 12, such as hydroxylated lecithin, have also been developed, but there are concerns about harm because they are easily converted into harmful peroxides, and the hydroxyl groups prevent the hydrophobic tails from aligning side by side Therefore, it is disadvantageous in forming stable liposomes. As a hydrophilic lecithin product that does not use a chemical method, there is a hydrolyzed lecithin or lysolecithin prepared through a hydrolysis reaction. Hydrolyzed lecithin is a material in which one of the hydrophobic tails of lecithin is decomposed with phospholipase A2. It has high biocompatibility due to low cytotoxicity and 5% water solubility because it has a water solubility of 10% or more. Eggplant has the advantage of being a product with improved performance as an emulsifier compared to the existing lecithin. However, there is a limitation in that it is not suitable as a raw material for a liposome formulation because it has a characteristic of forming a micelle structure instead of a liposome.

On the other hand, in order to develop a liposome formulation with high formulation stability and improved emulsification capacity, it is expected that a process for exchanging fatty acids with shorter fatty acids instead of hydrolysis in which they are completely decomposed can be developed, which will help solve this problem. In this regard, there is a process called transesterification. Hydrolysis breaks the ester bond into alcohol and fatty acid and consumes water, but transesterification replaces the fatty acid bound to the ester with another free fatty acid in the reaction solution to generate a new ester without exchanging water molecules. The transesterification process is advantageous in that it is not necessary to control the moisture in the reaction solution and that the reaction is possible even in a non-aqueous solvent. This process is mainly used in the field of synthesis of vitamin derivatives or esters and biofuels, but there is no case applied to HLB control of lecithin.

1. US Patent Registration US6797673B1 2. US Patent Registration US7666618B2

1. Willem van Nieuwenhuyzen et al. (2008) Update on vegetable lecithin and phospholipid technologies. Eur. J. Lipid Sci. Technol. 110: 472-486 2. Dario M. Cabezas et al. (2015) sunflower lecithin mixtures. Eur. J. Lipid Sci. Technol. 117(7) (lecithins and HLB)

An object of the present invention is to provide a system for producing high HLB lecithin capable of producing lecithin with improved solubility and formulation stability by controlling the HLB of lecithin.

And, the technical problem to be achieved by the present invention is to provide a high HLB lecithin manufactured using the high HLB lecithin production system.

In addition, the technical problem to be achieved by the present invention is to provide a cosmetic composition, a food composition, a pharmaceutical composition, a fragrance composition or a liposome composition prepared using the high HLB lecithin.

The problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

According to embodiments of the present invention for achieving the above object, a reaction device for performing transesterification by adding a biocatalyst to a reaction solution containing standard lecithin and fatty acids; and a degreasing device for removing lipids from the transesterification reaction product.

The reaction solution may include the standard lecithin and the fatty acid in a mass ratio of 1:0.1 to 1:20.

The reaction solution may further include a reaction solvent within 100 times the mass of the standard lecithin.

The reaction solvent is acetone, 1-butanol (1-butanol), t-butanol (t-butanol), ethyl acetate (ethyl acetate), ethanol (ethanol), isopropyl acetate (isopropyl acetate), 2- Propanol (2-propanol), 1-propanol (1-propanol), methanol (methanol), methyl ethyl ketone (methyl ethyl ketone), acetonitrile (acetonitrile), acetic acid (acetic acid), cyclohexane (cyclohexane), di Methyl sulfoxide (dimethyl sulfoxide), ethylene glycol (ethylene glycol), heptane (heptane), isooctane (isooctane), methylcyclohexane (methylcyclohexane), 2-methyltetrahydrofuran (2-methyltetrahydrofuran), Methyl t-butyl ether, toluene, tetrahydrofuran, xylenes, benzene, chloroform, carbon tetrachloride, di Chloromethane, diethyl ether, petroleum ether, dichloroethane, di-isopropylether, dimethylformamide, dioxane (dioxane), dimethyl acetate, dimethoxyethane, hexane, N-methylpyrrolidinone, pentane and pyridine consisting of It may be any one or more selected from the group.

The reaction solution may further include moisture in an amount of 0.0001 to 10% by weight based on the total content of the reaction solution.

The fatty acid may include a chain-type carboxylic acid containing 1 to 24 carbons and having 12 or less double bonds.

The biocatalyst may be a transesterification enzyme having an activity of binding the fatty acid to the primary alcohol or secondary alcohol of glycerol.

The transesterification reaction enzyme may be at least one selected from the group consisting of esterase, lipase, phospholipase A1 and phospholipase A2.

The esterase is derived from the genus Mucor, from the genus Rhizopus, from the genus Penicillium, from the genus Aspergillus, from the genus Candida (Candida), derived from the genus Cromobacterium. (Chromobacterium) genus origin, Bulkholderia genus origin, Escherichia genus origin, Chlorella genus origin, Pseudomonas genus origin, Proteobacterium genus origin, Archidia Derived from the genus Acidianus, from the genus Caldanaerobacter, from the genus Fervidobacterium, from the genus Methanocaldococcus, from the genus Methanothermobacter, Pyrova From the genus Pyrobaculum, from the genus Pyrococcus, from the genus Pyrolobus, from the genus Sulfolobus, from the genus Thermoanaerobacter, from the genus Thermococcus Derived from, Thermoproteus (Thermoproteus) genus, Thermomyces (Thermomyces) genus derived, Bacillus (Bacillus) genus derived, Malassezia (Malassezia) genus derived and pig (Porcine) genus derived from any one or more selected from the group consisting of have.

The transesterification enzyme is adsorption to a carrier, entrapment, encapsulation, covalent bonding, and non-covalent bonding Any one or more methods selected from the group consisting of It may be used by being immobilized or used without being immobilized.

The transesterification may be carried out at a temperature of 15 to 80 ℃.

The reactor may be in the form of a batch reactor, a continuous stirred tank reactor (CSTR), or a packed-bed reactor.

The reaction device may be one in which one or more gases selected from the group consisting of nitrogen, argon and helium are supplied therein to block oxygen contact with the inside of the reaction solution or the surface of the reaction solution.

The degreasing apparatus may be in the form of a sedimentation tank, a batch reactor, or a continuous stirred tank reactor (CSTR).

The degreasing apparatus includes water, acetone, 1-butanol, t-butanol, ethyl acetate, ethanol, isopropyl acetate, 2-propanol, 1-propanol, methanol, methyl ethyl ketone, acetonitrile, acetic acid, cyclohexane , dimethyl sulfoxide, ethylene glycol, heptane, isooctane, methylcyclohexane, 2-methyltetrahydrofuran (2-methyltetrahydrofuran) ), methyl t-butyl ether, toluene, tetrahydrofuran, xylenes, benzene, chloroform, carbon tetrachloride , dichloromethane, diethyl ether, petroleum ether, dichloroethane, di-isopropylether, dimethylformamide, Dioxane, dimethyl acetate, dimethoxyethane, hexane, N-methylpyrrolidinone, pentane and pyridine Any one or more solvents selected from the group consisting of may be added to separate lecithin and fatty acids.

According to other embodiments of the present invention, there is provided a high HLB lecithin prepared using the manufacturing system of the present invention described above.

The high HLB lecithin may have an HLB value of 7 to 20.

The high HLB lecithin may have a water solubility of 5 to 30 g/mL at 25°C.

According to other embodiments of the present invention, there is provided a cosmetic composition, a food composition, a pharmaceutical composition, a fragrance composition or a liposome composition prepared using the high HLB lecithin of the present invention described above.

According to embodiments of the present invention, 5 to 90 mol% of lecithin of the total lecithin may be substituted with only fatty acid (short chain fatty acid). Through this, the conventional lecithin having an HLB of less than 7 can be adjusted to a high HLB lecithin having an HLB in the range of 7 to 20, preferably 8 to 15.

Unlike conventional lecithin, which has only 5% by weight of water solubility, this high HLB lecithin has improved water solubility to about 30% by weight, so it is possible to increase the content of liposomes when developing a liposome formulation, and use other types of hydrophilic surfactants. Since there is no need to mix, it has the advantage of expanding the range of formulations that can be composed only with lecithin.

In addition, since the bio-based reaction process is used, there is an advantage of high biocompatibility because an organic synthesis reaction catalyst with high cytotoxicity is not used.

1 is a reaction scheme showing a reaction in which a target component is prepared in a system for producing high HLB lecithin according to an embodiment of the present invention.
Figure 2 is a process flow chart made in the manufacturing system of high HLB lecithin according to an embodiment of the present invention.
3 is a graph showing a comparison of the water solubility of the conventional lecithin (standard lecithin) and high HLB lecithin according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Examples of the present invention to be described below are provided to more clearly explain the present invention to those of ordinary skill in the art, and the scope of the present invention is not limited by the following examples, The embodiment may be modified in many different forms.

The terminology used herein is used to describe specific embodiments, not to limit the present invention. As used herein, terms in the singular form may include the plural form unless the context clearly dictates otherwise. Also, as used herein, the terms “comprise” and/or “comprising” refer to a referenced shape, step, number, action, member, element, and/or group that specifies the existence of these groups. and does not exclude the presence or addition of one or more other shapes, steps, numbers, acts, elements, elements, and/or groups thereof. In addition, as used herein, the term “connection” not only means that certain members are directly connected, but also includes indirectly connected members with other members interposed therebetween.

In addition, in the present specification, when a member is said to be located "on" another member, this includes not only a case in which a member is in contact with another member but also a case in which another member is present between the two members. As used herein, the term “and/or” includes any one and any combination of one or more of those listed items. In addition, as used herein, terms such as "about", "substantially", etc. are used in the meaning of the range or close to the numerical value or degree, in consideration of inherent manufacturing and material tolerances, and to help the understanding of the present application The exact or absolute figures provided for this purpose are used to prevent the infringer from using the mentioned disclosure unfairly.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The size or thickness of the regions or parts shown in the accompanying drawings may be slightly exaggerated for clarity and convenience of description. Like reference numerals refer to like elements throughout the detailed description.

1 is a reaction scheme showing a reaction in which a target component is prepared in the high HLB lecithin manufacturing system according to an embodiment of the present invention, and FIG. 2 is a process made in the high HLB lecithin manufacturing system according to an embodiment of the present invention It is a flow chart.

1 and 2, in the system for producing high HLB (hydrophilic-lipophilic balance) lecithin according to an aspect of the present invention, a biocatalyst is added to a reaction solution containing standard lecithin and fatty acids. a reactor for performing transesterification; and a degreasing device for removing lipids from the reaction product of the transesterification.

Here, the standard lecithin may be obtained from plant seeds and/or fruits such as soybean (soybean), sunflower, and rapeseed, or obtained from purified natural raw materials such as egg yolk and/or animal yolk and/or lipids removed therefrom.

In addition, the reaction solution may include the standard lecithin as a main reactive substrate and the fatty acid as a secondary reactive substrate in a mass ratio of 1:0.1 to 1:20. When such a mass ratio is satisfied, the transesterification reaction may be properly performed.

In addition, the reaction solution may further include a reaction solvent within 100 times the mass of the standard lecithin.

The reaction solvent is acetone, 1-butanol (1-butanol), t-butanol (t-butanol), ethyl acetate (ethyl acetate), ethanol (ethanol), isopropyl acetate (isopropyl acetate), 2- Propanol (2-propanol), 1-propanol (1-propanol), methanol (methanol), methyl ethyl ketone (methyl ethyl ketone), acetonitrile (acetonitrile), acetic acid (acetic acid), cyclohexane (cyclohexane), di Methyl sulfoxide (dimethyl sulfoxide), ethylene glycol (ethylene glycol), heptane (heptane), isooctane (isooctane), methylcyclohexane (methylcyclohexane), 2-methyltetrahydrofuran (2-methyltetrahydrofuran), Methyl t-butyl ether, toluene, tetrahydrofuran, xylenes, benzene, chloroform, carbon tetrachloride, di Chloromethane, diethyl ether, petroleum ether, dichloroethane, di-isopropylether, dimethylformamide, dioxane (dioxane), dimethyl acetate, dimethoxyethane, hexane, N-methylpyrrolidinone, pentane and pyridine consisting of It may be any one or more selected from the group, but is not particularly limited as long as it is a solvent in which the transesterification reaction according to the present invention can be performed.

In addition, the reaction solution may further contain 0.0001 to 10% by weight of water based on the total content of the reaction solution. can be done more efficiently.

On the other hand, the fatty acid may be a low molecular weight fatty acid, in this case, the low molecular weight fatty acid may be composed of 1 to 24 carbon atoms, preferably including 1 to 16 carbon atoms. And, it may include a chain-type carboxylic acid having 12 or less double bonds between the carbons.

In addition, the fatty acid may be supplied to the reactor by itself, but may be supplied to the reactor in the form of a metal salt of a fatty acid or an ester compound of a fatty acid, and the supply form is not particularly limited.

On the other hand, the biocatalyst may be a transesterification enzyme having an activity of binding the fatty acid to the primary alcohol or secondary alcohol of glycerol.

At this time, the biocatalyst is a transesterification enzyme having an activity of binding fatty acids to the primary alcohol and/or secondary alcohol of glycerol in an oil containing 0.0001 to 10% by weight of water, an organic solvent, or a non-solvent environment. can be

Specifically, the transesterification reaction enzyme may be any one or more selected from the group consisting of esterase, lipase, phospholipase A1 and phospholipase A2. In more detail, the esterase is derived from the genus Mucor, from the genus Rhizopus, from the genus Penicillium, from the genus Aspergillus, derived from the genus Aspergillus, Candida. genus origin, Chromobacterium genus origin, Burkholderia genus origin, Escherichia genus origin, Chlorella genus origin, Pseudomonas genus origin, Proteobacterium ( From the genus Proteobacterium, from the genus Acidianus, from the genus Caldanaerobacter, from the genus Fervidobacterium, from the genus Methanocaldococcus, derived from the genus Methanocaldococcus, Methanothermobacter ( Derived from the genus Methanothermobacter, from the genus Pyrobaculum, from the genus Pyrococcus, from the genus Pyrolobus, from the genus Sulfolobus, from the genus Thermoanaerobacter , Thermococcus (Thermococcus) genus derived, Thermoproteus (Thermoproteus) genus derived, Thermomyces (Thermomyces) genus derived, Bacillus (Bacillus) genus derived, Malassezia (Malassezia) genus derived and pig (Porcine) genus derived from the group consisting of It may be any one or more selected from.

Specifically, lipozyme 435 (Lipozyme 435) manufactured by Novozyme may be used as a biocatalyst.

As such, by using a biocatalyst, it is possible to exclude the use of an organic synthesis reaction catalyst with high cytotoxicity, so there is an advantage in that the biocompatibility of high HLB lecithin prepared therefrom can be improved.

And, the transesterification reaction enzyme, adsorption to a carrier (adsorption), entrapment (entrapment), encapsulation (encapsulation), covalent bonding (covalent bonding) and any one or more selected from the group consisting of non-covalent bonding (non-covalent bonding) It may be used by being immobilized through the method, or may be used without being immobilized.

In addition, the transesterification may be carried out at a temperature of 15 to 80 ° C., if it is less than the temperature range, the transesterification reaction may not be made, and if it exceeds the temperature range, an unwanted reaction occurs, It is undesirable because the yield of the product may be lowered.

On the other hand, the reaction apparatus, a batch reactor (batch reactor), continuous stirred tank reactor (CSTR; continuous stirred tank reactor), or may include the form of a packed-bed reactor (packed-bed reactor), but is not limited thereto.

And, in the reactor, any one or more gases selected from the group consisting of nitrogen, argon, and helium are supplied therein, so that oxygen existing in the reaction chamber can be removed. Thereby, it is possible to block the oxygen contact with the inside of the reaction solution or the surface of the reaction solution in the reactor, and through this, it is possible to prevent a side reaction from occurring.

In addition, the degreasing apparatus may include a sedimentation tank, a batch reactor, or a continuous stirred tank reactor (CSTR) type, but is not limited thereto.

In addition, the degreasing apparatus can be operated within a temperature range of -40 to 50 °C, and when the temperature range is satisfied, there is an advantage in that the lipid component can be effectively removed from the reaction product.

And, the degreasing apparatus is characterized in that by separating lecithin and lipids from the reaction product of transesterification to remove lipids, the degreasing apparatus includes water, acetone, 1-butanol, t-butanol, ethyl acetate, ethanol, isopropyl acetate, 2-propanol, 1-propanol, methanol ), methyl ethyl ketone, acetonitrile, acetic acid, cyclohexane, dimethyl sulfoxide, ethylene glycol, heptane (heptane), isooctane, methylcyclohexane, 2-methyltetrahydrofuran, methyl t-butyl ether, toluene, tetra Hydrofuran, xylene, benzene, chloroform, carbon tetrachloride, dichloromethane, diethyl ether, petroleum ether ( petrolenium ether) dichloroethane, di-isopropylether, dimethylformamide, dioxane, dimethyl acetate, dimethoxyethane , hexane (hexane), N-methylpyrrolidinone (N-methylpyrrolidinone), pentane (pentane) and selected from the group consisting of pyridine (pyridine) Any one or more solvents may be added to separate lecithin and fatty acids.

On the other hand, high HLB lecithin according to another embodiment of the present invention is characterized in that it was prepared using the above-described high HLB lecithin production system according to the present invention, in which case, the HLB value of high HLB lecithin is 7 to 20, Preferably it may be 8 to 15. In addition, the high HLB lecithin may be in a form in which two fatty acids are bound.

Unlike the conventional lecithin having an HLB of less than 7, the high HLB lecithin according to the present invention has the advantage that there is no need to separately mix other types of hydrophilic surfactants because water solubility is improved, and it is composed only of the high HLB lecithin according to the present invention. It has the advantage of widening the range of possible formulations.

Specifically, the high HLB lecithin may have a water solubility of 5 to 30 g/mL at 25°C.

Meanwhile, the composition according to another embodiment of the present invention may be a composition prepared using the high HLB lecithin according to the present invention described above.

The composition may be a cosmetic composition, a food composition, a pharmaceutical composition, a fragrance composition, or a liposome composition, but is not limited thereto, and in some cases, may be a paint composition.

In this case, the high HLB lecithin according to the present invention can be utilized as a surfactant among the formulations of the compositions, and water solubility is greatly improved compared to the conventional lecithin. Therefore, compared to the prior art, it is possible to prepare an emulsion or liposome with a high concentration.

Hereinafter, preferred examples are presented to help the understanding of the present invention. However, the following examples are only provided for easier understanding of the present invention, and the content of the present invention is not limited by the examples.

Example

A reaction substrate was prepared by mixing 100 g of standard lecithin with 500 g of acetic acid. Thereafter, 500,000 units of Lipozyme 435 (Lipozyme 435, Novozyme) were added, followed by reaction at 35° C. and 300 rpm. The reaction solution was sampled at 6, 12, 24, and 48 hours after initiation of the reaction. After vacuum drying the recovered precipitate, 0.5 g was quantified and diluted in ethanol to make 50 mL, and this was analyzed by HPLC. The analysis method of HPLC is as follows.

- Column: Waters Resolve C18 silica column 10㎛, 4.6 X 250mm

- Mobile phase: n-hexane:isopropanol:DW (30:60:8)

- Flow rate: 0.5 mL/min

- Detector: ELSD

- Carrier gas(N ₂ ) pressure: 30 psi

- Nebulization temp: 60 ℃

- Drift tube temp: 75 ℃

As a result of HPLC analysis, it was confirmed that, after 48 hours from the start of the reaction, fatty acids were substituted with acetate in 72 mol% of lecithin.

After concentrating the reaction solution, 200 mL of acetone was added to recover the precipitate at 4° C. to remove the fatty acid generated through the reaction. After the recovered precipitate was vacuum dried and analyzed by HPLC, it was confirmed that 98 mol% of the standard lecithin mol (mol) added before the reaction was recovered in the form of lecithin or high HLB lecithin.

comparative example

As standard lecithin, soybean lecithin (98% phosphatidylcholine) was prepared.

Experimental example

After quantifying 10 g of each of the lecithin prepared in Examples and Comparative Examples, water was added until completely dissolved while stirring at 25 ° C. at 200 rpm. As a result, the aqueous solubility of lecithin according to Examples was 22.8 g/ It was analyzed as 100mL, and the aqueous solubility of lecithin according to Comparative Example was analyzed as 4.5g/100mL (see FIG. 3). For reference, the formula for water solubility is as follows.

Water solubility (g/100mL) = {mass of lecithin (g)}/{amount of water added (mL)} * 100

In the present specification, preferred embodiments of the present invention have been disclosed, and although specific terms are used, these are only used in a general sense to easily describe the technical content of the present invention and help the understanding of the present invention, and to limit the scope of the present invention. It is not meant to be limiting. It is apparent to those of ordinary skill in the art to which the present invention pertains that other modifications based on the technical spirit of the present invention can be implemented in addition to the embodiments disclosed herein. For example, those of ordinary skill in the art may modify the high HLB lecithin production system according to the embodiment described with reference to FIGS. 1 to 3 and the high HLB lecithin prepared using the same. will be able to know In addition, it is understood that the high HLB lecithin manufacturing system according to the embodiment and the high HLB lecithin prepared using the same can be applied for various purposes in other fields other than cosmetic compositions, food compositions, pharmaceutical compositions, fragrance compositions, and liposome compositions. will be able Therefore, the scope of the invention should not be defined by the described embodiments, but should be defined by the technical idea described in the claims.

Claims (23)

a reaction device for performing transesterification by adding a biocatalyst to a reaction solution containing standard lecithin and fatty acids; and
A system for producing high HLB (hydrophilic-lipophilic balance; hydrophilic-lipophilic balance) lecithin comprising a degreasing device for removing lipids from the transesterification reaction product. According to claim 1,
The reaction solution, the standard lecithin and the fatty acid in a mass ratio of 1:0.1 to 1:20, a system for producing high HLB lecithin. The method of claim 1,
The reaction solution, high HLB lecithin production system further comprising a reaction solvent within 100 times the mass of the standard lecithin. 4. The method of claim 3,
The reaction solvent is acetone, 1-butanol (1-butanol), t-butanol (t-butanol), ethyl acetate (ethyl acetate), ethanol (ethanol), isopropyl acetate (isopropyl acetate), 2- Propanol (2-propanol), 1-propanol (1-propanol), methanol (methanol), methyl ethyl ketone (methyl ethyl ketone), acetonitrile (acetonitrile), acetic acid (acetic acid), cyclohexane (cyclohexane), di Methyl sulfoxide (dimethyl sulfoxide), ethylene glycol (ethylene glycol), heptane (heptane), isooctane (isooctane), methylcyclohexane (methylcyclohexane), 2-methyltetrahydrofuran (2-methyltetrahydrofuran), Methyl t-butyl ether, toluene, tetrahydrofuran, xylenes, benzene, chloroform, carbon tetrachloride, di Chloromethane, diethyl ether, petroleum ether, dichloroethane, di-isopropylether, dimethylformamide, dioxane (dioxane), dimethyl acetate, dimethoxyethane, hexane, N-methylpyrrolidinone, pentane and pyridine A system for producing high HLB lecithin at least one selected from the group. According to claim 1,
The reaction solution, the system for producing high HLB lecithin further comprising 0.0001 to 10% by weight of moisture relative to the total content of the reaction solution. According to claim 1,
The fatty acid is a high HLB lecithin production system comprising 1 to 24 carbons, and a chain carboxylic acid having 12 or less double bonds. According to claim 1,
The biocatalyst is a transesterification enzyme having the activity of binding the fatty acid to the primary alcohol or secondary alcohol of glycerol, high HLB lecithin production system. 8. The method of claim 7,
The transesterification enzyme is at least one selected from the group consisting of esterase, lipase, phospholipase A1 and phospholipase A2, high HLB lecithin system. 9. The method of claim 8,
The esterase is derived from the genus Mucor, from the genus Rhizopus, from the genus Penicillium, from the genus Aspergillus, from the genus Candida (Candida), derived from the genus Cromobacterium. (Chromobacterium) genus derived, Burkholderia genus derived, Escherichia genus derived, Chlorella genus derived, Pseudomonas genus derived, Proteobacterium genus derived, Archidia Derived from the genus Acidianus, from the genus Caldanaerobacter, from the genus Fervidobacterium, from the genus Methanocaldococcus, from the genus Methanothermobacter, Pyrova From the genus Pyrobaculum, from the genus Pyrococcus, from the genus Pyrolobus, from the genus Sulfolobus, from the genus Thermoanaerobacter, from the genus Thermococcus Derived from, Thermoproteus genus, Thermomyces genus derived, Bacillus genus derived, Malassezia genus derived, and pig (Porcine) genus derived from any one or more selected from the group consisting of HLB lecithin manufacturing system. 8. The method of claim 7,
The transesterification enzyme is, adsorption to a carrier, entrapment, encapsulation, covalent bonding (covalent bonding) and any one or more methods selected from the group consisting of non-covalent bonding (non-covalent bonding) A system for producing high HLB lecithin that is used by being immobilized or used without immobilization. According to claim 1,
The transesterification is a system for producing high HLB lecithin that is performed at a temperature of 15 to 80 ℃. According to claim 1,
The reaction apparatus is a batch reactor, a continuous stirred tank reactor (CSTR) or a packed-bed reactor. According to claim 1,
The reaction device is a system for producing high HLB lecithin in which one or more gases selected from the group consisting of nitrogen, argon and helium are supplied therein to block oxygen contact with the inside of the reaction solution or the surface of the reaction solution. According to claim 1,
The degreasing apparatus is a system for producing high HLB lecithin, including in the form of a sedimentation tank, a batch reactor, or a continuous stirred tank reactor (CSTR). According to claim 1,
The degreasing apparatus includes water, acetone, 1-butanol, t-butanol, ethyl acetate, ethanol, isopropyl acetate, 2-propanol, 1-propanol, methanol, methyl ethyl ketone, acetonitrile, acetic acid, cyclohexane , dimethyl sulfoxide, ethylene glycol, heptane, isooctane, methylcyclohexane, 2-methyltetrahydrofuran (2-methyltetrahydrofuran) ), methyl t-butyl ether, toluene, tetrahydrofuran, xylenes, benzene, chloroform, carbon tetrachloride , dichloromethane, diethyl ether, petroleum ether, dichloroethane, di-isopropylether, dimethylformamide, Dioxane, dimethyl acetate, dimethoxyethane, hexane, N-methylpyrrolidinone, pentane and pyridine A system for producing high HLB lecithin in which any one or more solvents selected from the group consisting of are added to separate lecithin and fatty acids. High HLB lecithin prepared using the manufacturing system of claim 1. 17. The method of claim 16,
The high HLB lecithin is high HLB lecithin having an HLB value of 7 to 20. 17. The method of claim 16,
The high HLB lecithin is high HLB lecithin having a solubility in water at 25°C of 5 to 30 g/mL. A cosmetic composition prepared using the high HLB lecithin of claim 16. A composition for food prepared using the high HLB lecithin of claim 16. A pharmaceutical composition prepared using the high HLB lecithin of claim 16. A fragrance composition prepared using the high HLB lecithin of claim 16. A liposome composition prepared using the high HLB lecithin of claim 16.

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