KR20220078026A - Culture Reactor Of Microbe For Wastewater Treatment And Cultivation Method Using thereof - Google Patents

Abstract

The present invention relates to an apparatus for culturing microorganisms for wastewater treatment and a culture method using the same, and more particularly, a raw water inlet 111 to which raw water is supplied is provided on one side and a treated water outlet 112 through which treated water is discharged on the other side. ) of the reactor body 100 having a predetermined shape provided; a partition wall 200 positioned in the internal space of the reaction tank body part 100; and a stirrer 300 provided in the inner space of the reaction tank main body 100; relates to a microorganism culture apparatus for wastewater treatment, characterized in that it comprises a, and a culturing method using the same.

Description

Culture Reactor Of Microbe For Wastewater Treatment And Cultivation Method Using thereof

The present invention relates to an apparatus for culturing microorganisms for wastewater treatment and a culture method using the same, and more particularly, to an apparatus for culturing anammox microorganisms that remove nitrogen from water by simultaneously ingesting ammonia and nitrous acid, and a culture method using the same.

Nitrogen flowing into the ecosystem is continuously accumulating according to industrial development, and the average total nitrogen inflow concentration in Korea has increased from 35.0 mg/L in 2012 to 36.2 mg/L in 2013.

The standards for effluent water quality from domestic public sewage treatment facilities are specified in Paragraph 1 of Article 3 (Water quality standards, etc.) of the 「Enforcement Regulations of the Sewerage Act」, and the revised [Water Quality Standards for Discharge from Public Sewage Treatment Facilities, Attached Table] in February 2020. 1], the current effluent quality consists of 7 items: BOD, COD, SS, TN, TP, and total coliform count. In the case of total nitrogen, a sewage treatment plant with a daily sewage treatment capacity of 50 m3 or less should treat it at 40 mg/L or less, and a sewage treatment plant of 50 m3 or more should treat it at a rate of 20 mg/L or less, regardless of location.

However, after January 1, 2021, a new standard will be applied. The analytical standard for chemical oxygen demand is changed from COD to TOC, and the standard value is also significantly strengthened. Although fortified 10 times below L, total nitrogen has been maintained at 20 mg/L for about 20 years since 2000.

This is because the vague judgment that total phosphorus, not total nitrogen, was the cause of mass generation of algae in public appeals, and it was relatively effective to control total phosphorus compared to total nitrogen. However, algae outbreaks still occur even after the total phosphorus discharge water quality regulation is strengthened, because the sole regulation of total phosphorus cannot effectively inhibit the growth of algae. Accordingly, experts judge that nitrogen emission standards will become stricter in the future.

The domestic eutrophication problem is expected to be an important cause of the increase in nitrogen and phosphorus outflow, but since most domestic sewage treatment plant designs are designed only for the purpose of removing organic matter and solid matter, the introduction of an advanced sewage treatment process capable of removing nitrogen and phosphorus It is essential.

There are various methods such as physicochemical methods and biological treatment methods for removing nitrogen in water, but the biological treatment method has the advantage of low secondary pollution and economical efficiency.

In particular, recently, water treatment using anaerobic ammonia oxidation (hereinafter, anamox) microorganisms has been in the spotlight. Anammox technology is being developed for high-intensity electronics industry wastewater treatment through partial nitrification and anammox process, and ecologically, it is being studied at the functional genome and transcription level with respect to the global nitrogen cycle occurring in the sea. .

Anamox microorganisms are autotrophic microorganisms that generate nitrogen gas by simultaneously ingesting ammonia and nitrous acid. By mixing and culturing with ammonia-Oxidizing Bacteria (AOB) at low oxygen concentration, the process can be simplified as well as external It has the advantage of reducing overall operating costs, such as not requiring carbon source supply.

However, since the growth rate of anammox microorganisms is slow, it is not easy to secure the amount of microorganisms, and in order to operate effectively, excellent sedimentation is required.

Korean Patent Publication No. 2014-0091997

An object of the present invention is to provide a culture apparatus and a culture method capable of effectively culturing anammox microorganisms having excellent sedimentation properties in order to solve the above problems.

The microorganism culture apparatus for wastewater treatment according to the present invention for achieving the above object is provided with a raw water inlet 111 through which raw water is supplied on one side and a treated water outlet 112 through which treated water is discharged on the other side. a reactor body 100 having a; a partition wall 200 positioned in the internal space of the reaction tank body part 100; and a stirrer 300 provided in the internal space of the reaction tank body part 100 .

In addition, in the microorganism culture apparatus for wastewater treatment of the present invention, the reaction tank body part 100 includes a reaction tank side part 110 , a reaction tank lower surface part 120 sealing the lower end of the reaction tank side part 110 , and the reaction tank side part 110 . ) is composed of the upper surface portion 130 of the reaction tank that seals the upper end, the horizontal cross-section has a wide egg-shaped area on one side and a relatively narrow area on the other side, like the shape of cutting an egg vertically, and the partition wall 200 is a first space portion (S1) and the second space (S2) are arranged to partition a wide area and a relatively narrow area of the reaction tank body part 100 to form, the upper edge is in close contact with the reaction tank upper surface part 130, the side edge is in close contact with the reaction tank side part 110, the lower edge is positioned to be spaced apart from the reaction tank lower surface part 120 by a predetermined distance, and the stirrer 300 is composed of a first stirrer 310 and a second stirrer 320, The first stirrer 310 is positioned in the first space S1, and the second stirrer 320 is positioned in the second space S2.

In addition, in the microorganism culture apparatus for wastewater treatment of the present invention, the microorganism for wastewater treatment is an anammox microorganism.

In addition, as a method for culturing microorganisms using the microorganism culture apparatus for wastewater treatment of the present invention, the microorganism culture apparatus is characterized in that it is operated as a continuous stirred tank reactor (CSTR).

In addition, as a method of culturing microorganisms using the microorganism culturing apparatus for wastewater treatment of the present invention, the microorganisms to be cultured are characterized in that they are of a granular type having an average size of 1 mm or more.

According to the microorganism culture apparatus for wastewater treatment and the culture method using the same according to the present invention, a continuously stirred tank using an egg-shaped reaction tank with a horizontal cross section wide as in the shape of an egg cut vertically and a relatively narrow area on the other side By operating in a reactor type, it is possible to obtain granular anammox microorganisms having an average particle diameter of 1 mm or more, so that not only has excellent sedimentation property, but also excellent nitrogen removal efficiency can be achieved even when dissolved oxygen is high.

1 is a perspective view of an apparatus for culturing microorganisms according to a preferred embodiment of the present invention.
2 is a plan view of the microorganism culture apparatus shown in FIG.
3 is a front view of a microorganism culturing apparatus according to Comparative Example 1.
4 is a photograph of anammox microorganisms cultured under the conditions of Example 1 and Comparative Example 1.
5 is a nitrogen removal result of anammox microorganisms cultured under the conditions of Example 1 and Comparative Example 1.
6 is a result of nitrogen removal according to whether or not dissolved iron is added.

Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings. Prior to this, the terms or words used in the present specification and claims should not be construed as being limited to their ordinary or dictionary meanings, and the inventor should properly understand the concept of the term in order to best describe his invention. Based on the principle that can be defined, it should be interpreted as meaning and concept consistent with the technical idea of the present invention.

Therefore, the embodiments described in this specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention, and do not represent all of the technical spirit of the present invention. It should be understood that there may be variations.

Hereinafter, a microorganism culturing apparatus for wastewater treatment and a culturing method using the same according to the present invention will be described with reference to the accompanying drawings.

1 is a perspective view of a microorganism culturing apparatus according to a preferred embodiment of the present invention, and FIG. 2 is a plan view of the microorganism culturing apparatus shown in FIG. 1 . As shown in FIGS. 1 and 2 , the microorganism culture apparatus of the present invention is configured to include a reaction tank body part 100 , a partition wall 200 , and a stirrer 300 .

First, the reaction tank body part 100 is to provide a space for culturing microorganisms while supplying raw water as a substrate, and the reaction tank side part 110, the reaction tank lower surface part 120 that seals the lower end of the reaction tank side part 110, and a reaction tank upper surface part 130 sealing the upper end of the reaction tank side part 110 .

Here, a raw water inlet 111 for supplying raw water containing a substrate serving as food for microorganisms to be cultured is provided at the lower end of one side of the side part 110 of the reactor, and the treated water outlet through which the treated water from which the substrate is removed is discharged at the upper end of the other side (112) is provided.

In addition, one or more sample outlets 113 may be additionally provided between the raw water inlet 111 and the treated water outlet 112 in a vertical direction to analyze the water quality in the reaction tank intermittently or non-intermittently.

One or more openings, specifically, the first opening 121 and the second opening 122 for supplying nitrogen, are provided in the lower surface of the reaction vessel 120 coupled along the lower edge of the side surface of the reaction vessel 110 by a predetermined distance. It may be provided in a spaced apart state.

Anamox microorganisms grow under anaerobic or anaerobic conditions, and may be exposed to air when the reactor is opened for internal inspection or sensor replacement. In this case, degassing or degassing may be achieved by supplying nitrogen through the first and second openings 121 and 122 for a predetermined time. Of course, it is self-evident that the first opening 121 and the second opening 122 may act as passages when the microorganisms are discharged.

One or more openings, specifically, the third opening 131 and the fourth opening 132, are spaced apart from each other by a predetermined distance in the upper surface 130 of the reaction vessel coupled along the upper edge of the side surface 110 of the reaction vessel. Various measurement sensors (not shown) for measuring water temperature, pH, dissolved oxygen, etc. inside the reaction tank are mounted through the third opening hole 131 and the fourth opening hole 132 .

On the other hand, when viewed from above, the reaction tank body 100 of the present invention has a horizontal cross-section in which one area is wide and the other area is relatively narrow, ie, an egg shape, like a shape in which an egg is cut vertically.

And the partition wall 200 has an upper edge in close contact with the reaction tank upper surface 130, and a side edge in close contact with the reaction tank side surface 110 so as to divide the reaction tank body 100 into a wide area and a relatively narrow area. , in addition, the lower edge is positioned to be spaced apart from the lower surface of the reactor 120 by a predetermined distance. As described above, since the partition wall 200 is provided in the egg-shaped reaction tank body portion 100, a first space portion S1 is formed in a wide area and a second space portion S2 is formed in a relatively narrow area, and the raw water inlet ( 111) is a structure in which the first space S1 and the treated water outlet directly communicate with the second space S2.

The stirrer 300 is composed of a first stirrer 310 and a second stirrer 320 , the first stirrer 310 is the aforementioned first space S1 , and the second stirrer 320 is a second space Located in the section S2, it induces the flow of microbial flocs while homogenizing the substrate concentration.

Although not shown in the drawings, a known heating means (not shown) such as a heating wire may be additionally provided on the outer surface of the reaction tank body 100 so that the temperature necessary for the inhabitation and proliferation of microorganisms is maintained.

The cross section of the microbial reaction tank is generally circular, square, or oval, but in the present invention, it is egg-shaped and further includes a partition wall 200 so that a wide area is a first space (S1), and a relatively narrow area is a second space By dividing into (S2), there is no dead-zone and it is easy to control the flow of the fluid, so it is possible to minimize the outflow of the cultured microbial flocs.

As a culturing method using the microorganism culturing apparatus having the structure as described above, after seeding microorganisms in the reactor body part 100, raw water, that is, a substrate serving as food is continuously supplied while treated water in which the substrate has been consumed is continuously supplied. It operates in a so-called continuous stirred tank reactor (CSTR), which discharges to a

Here, the microorganism is an anammox microorganism that simultaneously ingests ammonia and nitrous acid and converts it into nitrogen gas. As described above, the microorganism is operated under anaerobic conditions, and cultured for a predetermined period until the flocs of microorganisms grow into granules having an average size of 1 mm or more. do.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and the present invention is not limited thereto.

Example 1

Anamox microorganisms were cultured while the microorganism culture apparatus shown in FIG. 1 was operated as a continuous stirred tank reactor (CSTR).

Anamox microorganisms cultured in the T sewage treatment plant were used as seed microorganisms. The initial MLSS concentration was about 5600 mg/L, and ammonia nitrogen concentration 100 mg-N/L and nitrite nitrogen 100 mg-N/L as a substrate source were used. The containing synthetic wastewater was continuously introduced (Table 1).

And the hydraulic residence time (HRT) was operated while maintaining the temperature of the reactor at 35-37° C. for 24 hours, pH 7-8, and using a temperature controller and a heating wire.

substance name Concentration (mg/L) NH ₄ Cl 100 NaNO ₂ 100 NaHCO ₃ 95 KH ₂ PO ₄ 6 MgSO ₄ 7H ₂ O 12 CaCl ₂ ·2H ₂ O 48

Example 2

After seeding the anammox microorganisms obtained in Example 1 in the medium, the amount of nitrogen removal was investigated. At this time, the specific composition of the medium contains 4 mg-Fe/L of dissolved iron as shown in <Table 2>.

That is, while culturing under the conditions of Example 1, the anammox microorganism concentration at the time of 260 days was injected into the medium so that the concentration was 3 g/L, and then anaerobic conditions were induced by nitrogen aeration. After culturing for 12 hours at 35-37°C, the supernatant was collected and ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen were measured.

substance name Concentration (mg/L) NH ₄ Cl 100 NaNO ₂ 100 NaHCO ₃ 95 KH ₂ PO ₄ 6 MgSO ₄ 7H ₂ O 12 CaCl ₂ ·2H ₂ O 48 FeCl ₂ 4

Comparative Example 1

Except for the reactor used and the operation method, the rest was carried out in the same manner as in Example 1. That is, as shown in FIG. 3 , the anammox microorganisms were cultured while the microorganism culture apparatus having a circular cross section was operated as a Sequencing Batch Reactor (SBR).

The operating conditions of each cycle are Fill and react, React, Settling, Withdrawal, and Idle. It was driven for 24 hours.

Comparative Example 2

Except that dissolved iron was not contained in the medium, the rest were carried out under the same conditions as in Example 2.

Experimental Example 1

While culturing under the conditions of Example 1 and Comparative Example 1, anammox microorganisms (stereoscopic microscope) were photographed at 260 days elapsed, and the results are shown in FIG. 4 .

In the case of the microorganism cultured according to Example 1 (FIG. 4 (a)), it can be expected that the loss of microorganisms can be minimized due to excellent sedimentation in the form of granules with an average of 1 mm or more, whereas the microorganism obtained in Comparative Example 1 ((b) of FIG. 4) was only around 0.1 mm.

Experimental Example 2

The amount of nitrogen removal according to whether or not contact with dissolved oxygen was evaluated for the anammox microorganisms isolated at the time of 260 days elapsed while culturing under the conditions of Example 1 and Comparative Example 1, and the results are shown in FIG. 5 .

The size of the separated microorganisms is that the microorganisms cultured according to Example 1 are in the form of granules with an average of 1 mm or more, the microorganisms obtained in Comparative Example 1 are flocs of approximately 0.1 mm, and dissolved oxygen 7.5 mg/L for each microorganism was acclimatized for 12 hours under conditions of anaerobic conditions (nitrogen aeration).

Thereafter, the synthetic raw water having the same composition as in <Table 1> was seeded so that the amount of microorganisms acclimatized to each condition was 3 g/L.

As shown in FIG. 5 , when not exposed to dissolved oxygen, the nitrogen removal amount of the microorganisms cultured according to Example 1 and Comparative Example 1 was within an error range and showed no significant difference.

However, when exposed to dissolved oxygen, the nitrogen removal amount of the microorganism according to Example 1 was 154 mg-N/L, whereas when the microorganism according to Comparative Example 1 was used, it was only 55 mg-N/L.

When operating the anammox process, oxygen should be aerated at about 2.0 mg/L so that ammonia-Oxidizing Bacteria (AOB) oxidizes ammonia nitrogen. Under these conditions, the presence of dissolved oxygen inhibits the anammox microorganisms. may act as a factor.

However, it can be confirmed that the microorganisms cultured according to Example 1 of the present invention maintain excellent nitrogen removal ability even in contact with a high concentration of dissolved oxygen.

Experimental Example 3

Under the conditions of Example 2 and Comparative Example 2, the amount of nitrogen removed at the time of 12 hours was confirmed, and the results are shown in FIG. 6 .

In the medium containing dissolved iron (Example 2), 201.06 mg/L of nitrogen was removed, whereas in the medium without dissolved iron (Comparative Example 2), 190.262 mg/L of nitrogen was removed. It was confirmed that the activity of the MOX microorganism was improved.

As described above, although the present invention has been described with reference to limited embodiments and drawings, the present invention is not limited thereto, and the technical idea of the present invention and the following by those of ordinary skill in the art to which the present invention pertains. Of course, various modifications and variations are possible within the scope of equivalents of the claims to be described.

100: reaction tank body part
110: reaction tank side part
111: raw water inlet 112: treated water outlet
113: sample outlet
120: reaction tank lower part
121: first opening 122: second opening
130: reaction tank upper surface
131: third opening hole 132: fourth opening hole
200: bulkhead
300: agitator
310: first stirrer
320: second stirrer
S1: first space part
S2: second space part

Claims (6)

a reactor body 100 having a predetermined shape provided with a raw water inlet 111 through which raw water is supplied on one side and a treated water outlet 112 through which treated water is discharged on the other side;
a partition wall 200 positioned in the inner space of the reaction tank body part 100; and
A microbial culture apparatus for wastewater treatment, characterized in that it comprises a;
According to claim 1,
The reaction tank body part 100 includes a reaction tank side part 110, a reaction tank lower surface part 120 for sealing the lower end of the reaction tank side part 110, and a reaction tank upper surface part 130 for sealing the upper part of the reaction tank side part 110. It is composed, but the horizontal cross section is egg-shaped with a wide area on one side and a relatively narrow area on the other side, like the shape of cutting an egg vertically,
The partition wall 200 is disposed to partition a wide area and a relatively narrow area of the reaction tank body 100 so that a first space portion S1 and a second space portion S2 are formed, and the upper edge is the upper surface of the reaction tank It is in close contact with the part 130, the side edge is in close contact with the reaction tank side part 110, and the lower edge is positioned to be spaced apart from the reaction tank lower surface part 120 by a predetermined distance,
The stirrer 300 includes a first stirrer 310 and a second stirrer 320 , the first stirrer 310 is located in the first space S1 , and the second stirrer 320 is the second stirrer 320 . 2 A microorganism culture apparatus for wastewater treatment, characterized in that it is located in the space (S2).
3. The method of claim 2,
The microorganism culture apparatus for wastewater treatment, characterized in that the microorganism for wastewater treatment is an anammox microorganism.
As a microorganism culturing method using the microorganism culturing apparatus for wastewater treatment according to claim 2 or 3,
The microorganism culture method for wastewater treatment, characterized in that the microorganism culture device is operated as a continuous stirred tank reactor (CSTR, continuous stirred tank reactor).
5. The method of claim 4,
The cultured microorganism is a microorganism culture method for wastewater treatment, characterized in that the granular type having an average size of 1 mm or more.
5. The method of claim 4,
The cultured microorganism is a microorganism culture method for wastewater treatment, characterized in that the granular type having an average size of 1 mm or more.

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