KR20220074809A - UV block for animal with skin and fur comprising mixture of plant extract as effective component - Google Patents

Abstract

The present invention relates to a quasi-drug for UV protection for coat animals comprising a mixture of Rose of Jericho ( Selaginella lepidophylla ) extract, broccoli extract, and Icelandic moss extract as an active ingredient. and non-irritating to the skin such as edema.

Description

UV block for animal with skin and fur comprising mixture of plant extract as effective component

The present invention relates to a sunscreen for coat animals comprising a mixture of plant extracts as an active ingredient.

Skin hygiene, which is the first barrier to the external environment of the living body, is very important for the health of companion animals. Because the skin of fur animals is weaker than humans and the nature of the skin is different, even if the ingredients are suitable for humans, they are often not suitable for coat animals. . In the case of removing the hair of the fur animal as described above, when the skin from which the hair is directly removed is exposed to sunlight, various skin diseases may be caused. In the case of animals, sunscreens are needed for the prevention of skin diseases rather than for skin whitening. For example, in the case of dogs (dogs), the epidermis of the skin consists of 3 to 5 layers, which is weaker than human skin, and the nature of the skin is weakly acidic in the case of humans, whereas in the case of dogs, it has weak alkaline properties.

As such, for reasons such as removing hair for human pleasure, there is a problem in that the number of times the skin of a dog or cat is directly exposed to ultraviolet rays increases. However, skin protectants applied to companion animals and the like are still at the level of moisturizing agents or cleaning agents.

On the other hand, Korean Patent Publication No. 2020-0065711 discloses a composition for improving animal skin diseases, and Korean Patent Publication No. 2020-0016691 discloses a natural composition used for improving skin diseases and antibacterial in pets, Korean Patent No. 2007762 discloses a composition for managing the fur of companion animals, which is made with an active ingredient mixed with a natural product complex extract and deep sea bedrock mineral water based on the extracellular matrix extracted from the pig bladder endothelium. There is no disclosure of a sunscreen for coat animals comprising a mixture of a plant extract of the present invention as an active ingredient.

The present invention has been derived from the above needs, and the present invention provides a sunscreen for coat animals comprising a mixture of plant extracts as an active ingredient, and the active ingredient not only blocks UV rays, but also skin erythema, scab and edema By confirming that it does not irritate the skin, the present invention has been completed.

In order to achieve the above object, the present invention provides a quasi-drug for protecting the skin of a coat animal comprising a mixture of Rose of Jericho ( Selaginella lepidophylla ) extract, broccoli extract, and Icelandic moss extract as an active ingredient.

The present invention relates to a sunscreen for coat animals comprising a mixture of plant extracts as an active ingredient. It is effective in preventing skin erythema, crusting, and edema.

1 is a result of observing erythema and edema for 24 to 72 hours after administering the moisture mist of the present invention to abrasion sites.
Figure 2 is the result of observing erythema and edema for 24 to 72 hours after administering the sunscreen for pets of Comparative Example to the abrasion site.
3 is a result confirming the UV B blocking effect of a mixture of Rose of Jericho ( Selaginella lepidophylla ) extract, broccoli extract, and Icelandic moss extract of the present invention.

The present invention relates to a quasi-drug for protecting the skin of a coat animal comprising a mixture of an extract of Rose of Jericho ( Selaginella lepidophylla ), an extract of broccoli and an Icelandic moss extract as an active ingredient.

In addition to the active ingredient mixture, butylene glycol, saccharide isomerate, avocado fruit extract, coconut palm fruit extract, wormwood extract, propanediol, polyglyceryl-10 laurate, glycerin, jojoba seed extract, mexican chia seed extract, basil seed extract, esculinta lentil seed extract, port marigold extract, allantoin, chamomile flower oil, atlas cedar oil, 1,2-hexanediol, coconut palm tree, fructan, olive extract, setrol, diol Sodium EDT, Aloe Vera Leaf Extract, Dipropylene Glycol, Shea Butter, Olive Oil, Caprylic/Capric Triglyceride, Black Sesame Extract, Eye Horse Riding Extract, Thief's Wand Root Extract, Mulberry Bark Extract, Spanish Licorice Root Extract, Peony Root Extract, Mud Mushroom Extract, Angelica Root, Polyglyceryl-10 Stearate, Succulent Root Extract, Golden Extract, Squalane, Panthenol, Sodium Ascorbyl Phosphate, Hydrogenated Lecithin, Longi Pollen, Sodium Citrate , Citric Acid, Avocado Oil, Coconut Palm Duck, Jojoba Oil, Niacinamide, Ceramide NP, Cholesterol, Biotin, Folic Acid, Pyridoxine, Phytosphingosine, Cyanocobalamine, Tocopherol, Linoleic Acid, Fiboflavin , beta-carotene and thiamine HCL may be further included.

The extraction solvent for each of the extracts is preferably water, a C ₁ to C ₄ lower alcohol or a mixture thereof, and more preferably a water extract, but is not limited thereto.

Extracting each natural product (plant) by adding an extraction solvent;

2) filtering the extract of step 1); and

3) Concentrating the filtered extract of step 2) under reduced pressure and drying to prepare an extract.

In step 1), the extraction solvent is preferably water, C ₁ to C ₄ lower alcohol, or a mixture thereof, but is not limited thereto.

In the above preparation method, the extraction may use any conventional method known in the art, such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction. It is preferable to use a vacuum vacuum concentrator or a vacuum rotary evaporator for the concentration under reduced pressure in step 3), but is not limited thereto. In addition, drying under reduced pressure, vacuum drying, boiling drying, spray drying or freeze drying is preferable, but is not limited thereto.

Preferably, the skin protection of the coat animal is skin protection against ultraviolet B, but is not limited thereto.

The coat animal is preferably a dog or a cat, but is not limited thereto.

Hereinafter, the present invention will be described in more detail using examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited thereto.

Example 1. Manufacture of sunscreen for fur animals

88% by weight of Rose of Jericho ( Selaginella lepidophylla ) extract, 0.1% by weight of broccoli extract, and 0.05% by weight of Icelandic moss extract as active ingredients As a sunscreen for coat animals, a moisture mist was prepared. In addition to the above active ingredients, butylene glycol, saccharide isomerate, avocado fruit extract, coconut palm fruit extract, wormwood extract, propanediol, polyglyceryl-10 laurate, glycerin, jojoba seed extract, mexican chia seed extract, basil Seed Extract, Esculinta Lentils Seed Extract, Port Marigold Extract, Allantoin, Chamomile Flower Oil, Atlassida Wood Oil, 1,2-Hexanediol, Coconut Palm Tree, Fructan, Olive Extract, Setrol, Disodium EDIT A, Aloe Vera Leaf Extract, Dipropylene Glycol, Shea Butter, Olive Oil, Caprylic/Capric Triglyceride, Black Sesame Extract, Eye Horse Riding Extract, Thief's Wand Root Extract, Mulberry Bark Extract, Spanish Licorice Root Extract, Peony Root Extract, Mud Mushroom Extract, Angelica Root, Polyglyceryl-10 Stearate, Succulent Root Extract, Golden Extract, Squalane, Panthenol, Sodium Ascorbyl Phosphate, Hydrogenated Lecithin, Longi Pollen, Sodium Citrate, Citric Acid, avocado oil, coconut palm duck, jojoba seed oil, niacinamide, ceramide NP, cholesterol, biotin, folic acid, pyridoxine, phytosphingosine, cyanocobalamine, tocopherol, linoleic acid, fiboflavin, beta- A moisture mist was prepared to contain 0.15% by weight of carotene and thiamine HCL, and 2.7% by weight of distilled water. The extracts used water as a solvent.

As a comparative example, a liquid pet sunscreen containing distilled water, DMDM hydrate, glycol triotonite, propylene glycol, and dicaprylate/dicaprate was prepared.

Example 2. Analysis of skin irritation

[Experimental animals]

(1) strain and species: NZW-Rabbit (Clean animal)

(2) Gender and age: female, 4 months old;

(3) The weight of the model animal at the time of drug administration: 2.2~2.5kg

[Breeding Environment]

This test was conducted in the non-rodent animal breeding room of the Hoseo University Safety Evaluation Center set under environmental conditions of temperature 20±3℃, relative humidity 50±20%, ventilation frequency 10-15 times/hour, illumination cycle 12 hours, and illuminance 150-300 Lux. Experimental animals were bred in

After obtaining the test animal, it was used for the experiment after confirming that there was no abnormality such as weight loss during the acclimatization process for at least 7 days.

Composition of the experimental group experimental group number of animals Applicable substances compartment Dosage (ml) G1 3 sterile distilled water abrasion 0.5 3 biceps 0.5 3 Moisture mist of the present invention abrasion 0.5 3 biceps 0.5 G2 3 sterile distilled water abrasion 0.5 3 biceps 0.5 3 Sunscreen for pets (comparative example) abrasion 0.5 3 biceps 0.5

The dosage of the test substance used in this example is the liquid dosage recommended by the Ministry of Agriculture, Food and Rural Affairs (No. 2016-22, 2016.03.09) and OECD Acute Dermal Irritation/Corrosion (OECD 404, 2015. 07. 28) 0.5 ml of phosphorus was used, and 24 hours before the test substance was applied, the hair on the back was removed to a width of 15 × 15 cm using an animal electric epilator. It was divided into upper and lower parts, and the lower part was used as a test substance administration compartment, and the upper part was used as a control compartment. The administration compartment and the control compartment were again divided into non-scratch and abrasion sites, and the same abrasion sites were distributed diagonally to each other. Each area was 3x2cm wide and the boundary of the area was marked with an oil-based pen. In the abrasion site, using the tip of a syringe needle, the epidermis was damaged but the dermis was not damaged enough to prevent bleeding (Table 1).

On the upper side (control compartment), 3×2 cm gauze coated with sterile distilled water (0.5 mL) was applied, and on the lower side, gauze coated with a test substance (0.5 mL) was applied to the skin. To prevent loss of the test substance, it was covered with non-irritating tape (Tegarderm, 3M) on the gauze and fixed to the skin with a medical bandage, and removed after 4 hours. At this time, the administration site was washed with sterile distilled water in lukewarm water in a range that did not affect the test results so that no test substance remained, and the day of administration was set as Day 0.

For all treated animals, death and clinical symptoms were observed individually at least once a day from the time of administration to the end of the test.

(1) Weighing

Body weight was measured once before administration, on the day of administration, and on days 1 and 3 after initiation of administration.

As a result, no dead animals were observed during the test period, no specific symptoms by the test substance were observed during the test period, and no change in body weight was observed (Table 2).

Weight change by the moisture mist of the present invention and the pet sunscreen of the comparative example Weight (kg) Day 0 Day 1 Day 3 Moisture mist of the present invention 2.394±0.080 2.414±0.106 2.475±0.061 Comparative example pet sunscreen 2.444±0.095 2.45±0.050 2.510±0.107

(2) Observation of skin reaction

At 24, 48, and 72 hours after application of the test substance, the presence or absence of skin erythema, crusting, and edema of all animals was evaluated. The evaluation of the skin lesions at the application site was observed and evaluated according to the 'Evaluation Rating Table for Skin Reaction' disclosed in Table 3.

Skin reaction rating table Erythema and crust formation no erythema at all 0 Very mild erythema (only visible to the naked eye) One clear erythema 2 mildly severe erythema 3 Severe erythema (blushless redness) and mild crusting (deep damage) 4 Total possible erythema score 4 the formation of edema no swelling at all 0 Very mild edema (only visible to the naked eye) One Mild edema (if the margins are clearly demarcated due to pronounced swelling_ 2 Moderate edema (if the swelling is about 1 mm) 3 Severe edema (swelling more than about 1 mm and extending outside the exposed area) 4 Total possible edema score 4

As a result, no skin reaction was observed in the non-abrasive and abrasive regions where sterile distilled water was applied. Erythema and edema were not observed for 72 hours at the non-abrasion site applied with the moisture mist of the present invention, and very mild erythema was observed in 2 animals 24 hours after exposure to the test substance at the abrasion site, but from 48 hours on, all subjects No erythema was observed in Edema at the abrasion site was not observed in all subjects (FIG. 1).

Erythema and edema were not observed for 72 hours at the non-abrasion site applied with the pet sunscreen of the comparative example, but erythema was observed in 2 animals 24 hours after exposure to the test substance in the abrasion site, and one of them was observed up to 72 hours. Clear erythema was observed, and no erythema was observed on the 4th day, and no erythema was observed from the 48th hour on the other animal. In addition, edema was observed in one animal immediately after exposure to the test substance for 4 hours, but no edema was observed in all subjects from 24 hours to 72 hours ( FIG. 2 ).

(3) Determination of skin irritation

The skin irritation determination for the test substance was evaluated by using the primary skin irritation index, as shown in Table 4, by 'skin irritation determination index'. The primary skin irritation index was taken as the average value by adding the erythema and edema scores of all treated animals for each observation period.

Skin irritation determination index Primary skin irritation index classification 0.0-0.5 non-irritating 0.6~2.0 pharmaceutical irritant 2.1~5.0 Moderate irritation 5.1~8.0 strong irritant

In this Example 2, in order to investigate the degree of skin irritation or corrosiveness of the sunscreen mist for dogs, the test material was applied to the skin of a female white rabbit for 4 hours and then observed for 72 hours, and the degree of skin irritation of the test material was confirmed. .

As a result, the primary skin irritation index (P.I.I.) observed for 72 hours after application of the moisture mist according to the present invention and the comparative example pet sunscreen was calculated as 0.167 and 0.584, respectively, so the moisture mist and pet sunscreen of the present invention were non-irritating. considered to be material.

Example 3. UV protection performance test

In Example 3, the UV protection performance index SPF (Sun Protection Factor) index for the moisturizing mist according to the present invention and the pet sunscreen as a comparative example was confirmed.

MPF (Monochromatic Protection Factor) is the ability to protect against ultraviolet wavelengths, meaning the same ability as the SPF index, and the higher the MPF index, the higher the UV protection index. In addition, SPF index (MPF index) 2 is to block 50% UV rays, and in the case of coat animals, it is known that only 50% UV protection is safe, so 50% UV protection was used as a standard, and the results measured in Example 3, The average MPF index in the wavelength range of 290-320 nm, which is the UVB region, was found to be 2.3-2.5, and it was determined that there was a 50% UV-blocking effect.

Claims (5)

Rose of Jericho ( Selaginella lepidophylla ) A quasi-drug for protecting the skin of a coat animal comprising a mixture of extract, broccoli extract, and Icelandic moss extract as an active ingredient. According to claim 1, in addition to the mixture, butylene glycol, saccharide isomerate (Saccharide Isomerate), avocado fruit extract, coconut palm fruit extract, wormwood extract, propanediol, polyglyceryl-10 laurate, glycerin, Jojoba seed extract, Mexican chia seed extract, basil seed extract, esculinta lentil seed extract, port marigold extract, allantoin, chamomile flower oil, atlassida wood oil, 1,2-hexanediol, coconut palm, fructan, Olive Extract, Setrol, Disodium EDIT, Aloe Vera Leaf Extract, Dipropylene Glycol, Shea Butter, Olive Oil, Caprylic/Capric Triglyceride, Black Sesame Extract, Eye Horse Riding Extract, Thief's Wand Root Extract , Mulberry Bark Extract, Spanish Licorice Root Extract, Peony Root Extract, Mud Mushroom Extract, Angelica Root, Polyglyceryl-10 Stearate, H. Root Extract, Golden Extract, Squalane, Panthenol, Sodium Ascorbyl Phosphate, Hydrogenated Lecithin , Longi Pollen, Sodium Citrate, Citric Acid, Avocado Oil, Coconut Palm Duck, Jojoba Oil, Niacinamide, Ceramide NP, Cholesterol, Biotin, Folic Acid, Pyridoxine, Phytosphingosine, Cyanocobalamine, Tocopherol, Linoleic acid, fiboflavin, beta-carotene, and quasi-drugs for protecting the skin of fur animals further containing thiamine HCL. According to claim 1, wherein the extraction solvent of the extract is water, C ₁ ~ C ₄ A quasi-drug for skin protection of animals, characterized in that the lower alcohol or a mixture thereof. The quasi-drug for protecting the skin of a fur animal according to claim 1, wherein the skin protection of the coat animal is skin protection against UVB. The quasi-drug for protecting the skin of a fur animal according to claim 1, wherein the coat animal is a dog or a cat.

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