KR20220072167A - Scafold for restoration of cartilage - Google Patents

Abstract

The present invention is a plate-shaped base; and a cartridge connected to one surface of the base and formed in a hollow shape to contain a plurality of compositions constituting a cartilage culture inducing material in a plurality of layers therein; It is possible to provide a scaffold for cartilage regeneration, characterized in that it becomes.

Description

Scaffold for cartilage regeneration {SCAFOLD FOR RESTORATION OF CARTILAGE}

The present invention relates to a scaffold for cartilage regeneration.

Cartilage (cartilage) is a tissue composed of chondrocytes and a large amount of matrix surrounding it, and is a concept corresponding to the tibia (or bone tissue), which is a bone containing calcium as a main component. The action of cartilage includes preventing friction of the epiphysis as seen in the articular cartilage covering the epiphysis of the joint, maintaining elasticity such as organs and auricles, and providing resistance to pressure such as costal cartilage and pubis cartilage. can Cartilage is aneurysmal, avascular, and non-lymphatic, and once damaged, it cannot be restored to its original state. For example, cartilage within joints such as the knee, hip joint, and temporomandibular joint may be damaged by shock absorption or wear due to degenerative arthritis, and in this case, the damaged cartilage is not naturally restored.

Therefore, when cartilage is worn or damaged, it is necessary to replace the worn or damaged cartilage using an artificial cartilage member, cartilage collected from the body, or artificially cultured cartilage.

However, in the case of artificially culturing cartilage at the laboratory level, only artificial growth factors are used for culturing the cartilage, and the growth factors used are also limited. In most cases, it is inappropriate in terms of quality to be applied to the human body, and the three-dimensional culture is very difficult and the growth rate is also not satisfactory.

For example, when chondrocytes were isolated and cultured in vitro and cartilage replacement was performed, the tissue was weak compared to the existing cartilage in good condition, so it was difficult to expect its function. Thus, even when converted into cartilage, in the case of actual application, the tissue was not dense and crushed. That is, in the case of the conventional cartilage culture method at the laboratory level, there was a problem of very low efficiency compared to the high cost.

An object of the present invention is to provide a scaffold for cartilage regeneration that can be implanted in a site requiring cartilage regeneration to improve adhesion to surrounding normal cartilage.

In addition, the cartilage culture inducer is formed of three layers including stem cells, chondrogenesis inducers and growth factors, and the stem cells are sequentially absorbed with the chondrogenesis inducers and growth factors so that the stem cells absorb nutrients It is to provide a scaffold for cartilage regeneration that can grow by doing so.

Another object of the present invention is to provide a scaffold for cartilage regeneration that has directionality and can promote cartilage regeneration toward a specific surface.

In addition, it is to provide a scaffold for cartilage regeneration that can obtain the effect of inducing and promoting cartilage culture in a specific direction by including a cartridge for continuously discharging the cartilage culture inducing material on one surface of the base.

According to an embodiment of the present invention, a plate-shaped base; and a cartridge connected to one surface of the base and formed in a hollow shape to contain a plurality of compositions constituting a cartilage culture inducing material in a plurality of layers therein; including, implants in areas requiring cartilage regeneration It provides a scaffold for cartilage regeneration, characterized in that it becomes.

In addition, a plurality of compositions constituting the cartilage culture inducing material may provide a scaffold for cartilage regeneration, characterized in that it includes stem cells, chondrogenesis inducers, and growth factors.

In addition, it is possible to provide a scaffold for cartilage regeneration, characterized in that the stem cells, the chondrogenesis inducer and the growth factor are divided into three layers sequentially from top to bottom and accommodated in the cartridge inside.

In addition, the chondrogenesis inducer may provide a scaffold for cartilage regeneration, characterized in that it comprises at least one of Runx2 and Osterix.

In addition, the growth factor may provide a scaffold for cartilage regeneration, characterized in that it comprises at least one of TGF-b1, FGF, BMP-2, Hap and IGF.

In addition, it is possible to provide a scaffold for cartilage regeneration, characterized in that a differentiation enhancing material is formed between each layer of the stem cells, the chondrogenesis inducer and the growth factor.

In addition, in a state in which the stem cells, the chondrogenesis inducer and the growth factor form each layer, the layer between the stem cells and the chondrogenesis inducer is decomposed after the first time, and the stem cells are the cartilage Mixed with the generation inducer, the layer between the chondrogenesis inducer and the growth factor is decomposed after a second time, and the stem cells mixed with the chondrogenesis inducer are mixed with the growth factor A scaffold for cartilage regeneration can be provided.

In addition, on the outer surface of the cartridge, it is possible to provide a scaffold for cartilage regeneration, characterized in that the adhesion reinforcement material for increasing the adhesion to the tissue around the site where cartilage regeneration is required is formed.

In addition, the base and the cartridge may provide a scaffold for cartilage regeneration, characterized in that formed of a biodegradable material.

According to the embodiment of the present invention, it is possible to provide a scaffold for cartilage regeneration that can be implanted in a site requiring cartilage regeneration and can improve adhesion to surrounding normal cartilage.

In addition, the cartilage culture inducer is formed of three layers including stem cells, chondrogenesis inducers and growth factors, and the stem cells are sequentially absorbed with the chondrogenesis inducers and growth factors so that the stem cells absorb nutrients By doing so, it is possible to provide a scaffold for cartilage regeneration that can grow.

In addition, it is possible to provide a scaffold for cartilage regeneration that has directionality and can promote cartilage regeneration toward a specific surface.

In addition, it is possible to provide a scaffold for cartilage regeneration that can obtain the effect of inducing and promoting cartilage culture in a specific direction by including a cartridge for continuously discharging the cartilage culture inducing material on one surface of the base.

1 is a view showing a scaffold for cartilage regeneration according to an embodiment of the present invention;
Figure 2 is a view showing a cross-section of a cartridge in which the cartilage culture inducing material forming a plurality of layers is accommodated therein according to an embodiment of the present invention;
3 is a view showing that the scaffold for cartilage regeneration according to an embodiment of the present invention is inserted into the cartilage damaged site;
4 is a view showing a cross-section of a scaffold for cartilage regeneration according to an embodiment of the present invention;

Hereinafter, specific embodiments of the present invention will be described with reference to the drawings. However, this is merely an example, and the present invention is not limited thereto.

In the description of the present invention, if it is determined that the detailed description of the known technology related to the present invention may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted. In addition, the terms to be described later are terms defined in consideration of functions in the present invention, which may vary according to intentions or customs of users and operators. Therefore, the definition should be made based on the content throughout this specification.

The technical spirit of the present invention is determined by the claims, and the following examples are only one means for efficiently explaining the technical spirit of the present invention to those of ordinary skill in the art to which the present invention pertains.

1 is a view showing a scaffold 1 for cartilage regeneration according to an embodiment of the present invention.

Referring to FIG. 1 , the scaffold 1 for cartilage regeneration may be implanted in a site requiring cartilage regeneration. Cartilage regeneration scaffold (1) may include a base (10) and a cartridge (20).

The base 10 may be formed in a plate shape. Specifically, the base 10 may be formed in a plate shape to facilitate application to the affected area requiring cartilage regeneration.

The cartridge 20 is connected to one surface of the base 10, and is formed in a hollow shape so that a plurality of compositions constituting the cartilage culture inducing material 30 can be accommodated in a plurality of layers. Specifically, the cartridge 20 may be formed in a shape protruding from one surface of the base 10 in a cylindrical shape in one direction. However, the cartridge 20 is not limited to being formed in a cylindrical shape having a circular cross-section, and the cartridge 20 may be formed in various shapes such as a square, a pentagonal, and the like in cross-section.

A hollow inner space is formed inside the cartridge 20 so that the cartilage culture inducing material 30 can be accommodated.

The base 10 and the cartridge 20 may be made of a biodegradable material. For example, the biodegradable material may be any one of hyaluronic acid (glycosaminoglycan), alginate, fibrin, and collagen. In addition, the base 10 and the cartridge 20 may be formed integrally, and may be manufactured by bio 3D printing technology.

2 is a view showing a cross-section of the cartridge 20 in which the cartilage culture inducing material 30 forming a plurality of layers is accommodated therein according to an embodiment of the present invention.

Referring to FIG. 2 , the cartilage culture inducing material 30 made of a plurality of compositions may be accommodated inside the cartridge 20 . Specifically, the plurality of compositions constituting the cartilage culture inducing material 30 may include stem cells 31 , chondrogenesis inducers 32 , and growth factors 33 .

Here, the chondrogenesis inducer 32 may include at least one of Runx2 and Osterix. In addition, the growth factor 33 may include at least one of TGF-bi, FGF, BMP-2, Hap, and IGF.

The stem cells 31, the chondrogenesis inducer 32 and the growth factor 33 may be separated into three layers sequentially from the top to the bottom of the cartridge 20 and accommodated.

Specifically, based on that the portion connected to the base 10 is on the lower side, the growth factor 33 is accommodated in the lowermost layer, the chondrogenesis inducer 32 is accommodated in the middle layer, and the stem cells 31 are accommodated in the uppermost layer. ) can be accepted.

In addition, the differentiation enhancing material 34 may be formed between each layer of the stem cells 31 , the chondrogenesis inducer 32 , and the growth factor 33 . In the differentiation enhancing material 34, the stem cells 31, the chondrogenesis inducer 32 and the growth factor 33 are separated and accommodated in each layer, and as time passes, each layer is decomposed and the stem cells (31) may be mixed with the chondrogenesis inducer 32 to be differentiated, and then mixed with the growth factor 33 to be differentiated.

Specifically, in a state in which the stem cells 31 , the chondrogenesis inducer 32 , and the growth factor 33 form each layer and are accommodated in the cartridge 20 , it may be transplanted to a site requiring cartilage regeneration. After the first time in this state, the layer between the stem cells 31 and the chondrogenic flow factor 32 is decomposed so that the stem cells 31 are mixed with the chondrogenesis inducer 32, absorb nutrients, and differentiate can be

After the second time, which is a predetermined time longer than the first time, the layer between the chondrogenesis inducer 32 and the growth factor 33 is decomposed, and the stem cells 31 mixed with the chondrogenesis inducer 32 may be mixed with the growth factor 33 .

As such, as time passes, the stem cells 31 are mixed with the chondrogenesis inducing factor 32 and the growth factor 33, absorbing nutrients, and more stably bonding and cartilage can be generated at sites requiring cartilage regeneration. have.

3 is a view showing that the scaffold (1) for cartilage regeneration according to an embodiment of the present invention is inserted into the cartilage damaged site.

Referring to FIG. 3 , an adhesion reinforcing material 40 may be formed on the outer surface of the cartridge 20 . Specifically, the adhesion strengthening material 40 may be connected to the tissue around the site requiring cartilage regeneration to increase the adhesion of the cartridge 20 . That is, it is possible to improve the ability to attach to the normal cartilage around the cartridge 20 .

In addition, the scaffold 1 for cartilage regeneration according to the present invention is implanted in a site requiring cartilage regeneration, and can be inserted and implanted up to the subchondral bone, that is, the subchondral bone 53 .

That is, when the scaffold (1) for cartilage regeneration is implanted, the base 10 is implanted to be positioned on the subchondral bone 53, and the cartridge 20 is cartilage (normal cartilage) 51, cartilage It can be implanted so as to be hung over the boundary surface (tide mark) 52 of the sublingual bone.

4 is a view showing a cross-section of the scaffold 1 for cartilage regeneration according to an embodiment of the present invention.

Referring to FIG. 4 , the cartridge 20 may be formed to further include a cover portion 22 . Specifically, the cartridge 20 may include a body portion 21 and a cover portion 22 .

The body portion 21 is formed to protrude in one direction from one surface of the base 10 and is a portion in which the cartilage culture inducing material 30 is accommodated.

Here, referring to FIG. 4A , the cover part 22 is formed to protrude from the upper end of the body part 21 toward the inside of the body part 21 , and is inclined downward toward the inner center of the body part 21 . can be At this time, the cover part 22 does not completely shield the upper part of the body part 21 , and at the upper end of the body part 21 , the body part 21 may have an open center so that the inner space of the body part 21 can communicate with the outside. A predetermined length may be formed to protrude into the part 21 .

As such, the cover portion 22 is formed to be inclined downwardly into the body portion 21, thereby serving as a check valve. That is, as the cover part 22 is inclined downwardly to the inside, it is easy for the material outside the cartridge 20 to flow in, and the material inside the cartridge 20 can be induced to slowly flow out. Therefore, the supply rate of the cartilage culture inducing material 30 stored inside the cartridge 20 can be adjusted.

Here, referring to FIG. 4B , the cover part 22 may be formed to separate the inside of the body part 21 into three layers. Specifically, when the cartilage culture inducing material 30 in which each of the stem cells 31, the chondrogenesis inducer 32 and the growth factor 33 is separated into three layers is accommodated in the body portion 21, the cover The part 22 may be formed to protrude from three height points on the inner surface of the body part 21 so as to separate each layer of the stem cell 31 , the chondrogenesis inducer 32 and the growth factor 33 . That is, the cover part 22 may function as a check valve for each layer by dividing the inside of the body part 21 into three layers.

Although representative embodiments of the present invention have been described in detail above, those of ordinary skill in the art to which the present invention pertains will understand that various modifications are possible within the limits without departing from the scope of the present invention with respect to the above-described embodiments. . Therefore, the scope of the present invention should not be limited to the described embodiments, and should be defined by the claims and equivalents as well as the claims to be described later.

1: Scaffold for cartilage regeneration
10: base
20: cartridge
21: body part
22: cover part
30: cartilage culture inducer
31: stem cells
32: chondrogenesis inducer
33: growth factor
34: differentiation enhancing substance
40: adhesion strengthening material
51: cartilage (normal cartilage)
52: the interface between the cartilage and the sublingual bone (tide mark)
53: subchondral bone

Claims (9)

plate-shaped base; and
A cartridge that is connected to one surface of the base and is formed in a hollow shape to contain a plurality of compositions constituting a cartilage culture inducing material in a plurality of layers to be accommodated; including,
A scaffold for cartilage regeneration, characterized in that it is implanted in a site requiring cartilage regeneration.
The method according to claim 1,
A plurality of compositions constituting the cartilage culture inducing material is
A scaffold for cartilage regeneration, comprising stem cells, chondrogenesis inducers, and growth factors.
3. The method according to claim 2,
Inside the cartridge,
Cartilage regeneration scaffold, characterized in that the stem cells, the chondrogenesis inducer and the growth factor are divided into three layers sequentially from the top to the bottom and accommodated;
4. The method according to claim 3,
The chondrogenesis inducer is a scaffold for cartilage regeneration, characterized in that it comprises at least one of Runx2 and Osterix.
4. The method according to claim 3,
The growth factor is a scaffold for cartilage regeneration, characterized in that it comprises at least one of TGF-b1, FGF, BMP-2, Hap and IGF.
4. The method according to claim 3,
A scaffold for cartilage regeneration, characterized in that a differentiation enhancing material is formed between each layer of the stem cells, the chondrogenesis inducer and the growth factor.
4. The method according to claim 3,
In a state in which the stem cells, the chondrogenesis inducer and the growth factor form each layer,
After the first time, the layer between the stem cells and the chondrogenesis inducer is decomposed, and the stem cells are mixed with the chondrogenesis inducer,
After a second time, the layer between the chondrogenesis inducer and the growth factor is decomposed, and the stem cells mixed with the chondrogenesis inducer are mixed with the growth factor.
3. The method according to claim 2,
On the outer surface of the cartridge,
A scaffold for cartilage regeneration, characterized in that an adhesion-reinforcing material is formed to increase adhesion with tissues around a site requiring cartilage regeneration.
The method according to claim 1,
The base and the cartridge is a scaffold for cartilage regeneration, characterized in that formed of a biodegradable material.

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