KR20220061404A - magnetic rack device - Google Patents

Abstract

A magnetic rack device is provided. The magnetic rack device according to an exemplary embodiment of the present invention comprises: a holder member having a plurality of mounting holes formed at predetermined intervals so that a tube in which a certain amount of a mixture including a magnetic bead-containing material is stored can be individually mounted; and a main body including a pedestal and a magnetic fixing table movably coupled to the pedestal so that a plurality of permanent magnets are mounted at predetermined intervals and the position of the permanent magnets can be changed at a lower part of the holder member. According to the present invention, the position of the permanent magnets is changed according to the size and shape of the tube so that the permanent magnets are located at a position lower than the storage height of the magnetic bead-containing material included in the tube and thus the magnetic beads can be smoothly collected.

Description

magnetic rack device

The present invention relates to a magnetic rack device.

Molecular diagnostic testing involves a sample pre-treatment process of extracting nucleic acids from a sample. Polymerase Chain Reaction (hereinafter referred to as 'PCR') can amplify a large amount of genetic material with the same base sequence from a small amount of genetic material through the polymerase chain reaction, It can be used to diagnose various types of genetic diseases, or it can be applied to the DNA of bacteria, viruses, or fungi to diagnose infectious diseases.

PCR diagnostic kit using polymerase chain reaction uses magnetic beads such as magnetic nano silica particles to determine whether or not infection is present, and the accuracy of diagnosis is determined according to the structure and functional group of magnetic particles.

In such a diagnostic test using magnetic beads, the magnetic bead-containing material containing a certain amount of magnetic beads is mixed with the nucleic acid and reagent extracted from the sample, and then, after a certain period of time has elapsed, the magnetic bead-containing material is used using the magnetic force of a permanent magnet. It proceeds through the process of separating the magnetic bead from

In general, the magnetic bead-containing material containing a certain amount of magnetic beads is stored in a tube of a predetermined volume, although the amount of magnetic bead-containing material contained in the tube is the same, the size or shape of the tube varies depending on the manufacturer.

Accordingly, when the same amount of magnetic bead-containing material is stored in the tube, a deviation occurs in the storage height of the magnetic bead-containing material contained in the tube according to the size or shape of the tube.

For this reason, if one magnetic rack device is used to separate magnetic beads from the magnetic bead-containing material that has been reacted with the nucleic acid in the tube, the permanent magnet is higher than the storage height of the magnetic bead-containing material contained in the tube according to the size and shape of the tube. There is a problem in that it is not possible to smoothly collect the magnetic beads because it occurs in a high position.

The present invention has been devised in view of the above, and it is an object of the present invention to provide a magnetic rack device capable of changing the permanent magnet to a position where the magnetic beads in the tube can be smoothly collected even when the size and shape of the tube are different. .

In order to solve the above problems, the present invention provides a holder member having a plurality of mounting holes formed at predetermined intervals so that a tube in which a predetermined amount of a mixture containing a magnetic bead-containing material is stored can be individually mounted; and a body including a pedestal and a magnetic holder movably coupled to the pedestal so that a plurality of permanent magnets are mounted at a predetermined interval and the position of the permanent magnet can be changed at a lower position of the holder member. It provides a magnetic rack device that

In addition, the holder member may be detachably coupled to the main body via at least one fastening bar, the lower end of which is coupled to the pedestal.

In addition, the holder member may include at least one cutout extending from the mounting hole to allow entry of the tube when a tube having a relatively larger cross-sectional area than the mounting hole is inserted.

In addition, the plurality of mounting holes may be formed to have the same size as each other.

In addition, the pedestal may include a hollow protrusion extending upward from one surface to a certain height, and the magnetic holder may include a coupling portion inserted into the protrusion and a mounting portion to which the plurality of permanent magnets are fixed. , the mounting portion may be formed to have a relatively greater thickness than the coupling portion so that the downward movement through the upper edge of the protrusion can be constrained.

In addition, the permanent magnet may be coupled to the magnetic holder so that both ends are exposed to the outside.

In addition, the permanent magnet may be fixed to the magnetic holder so that one end side faces the side of the tube when the tube is inserted into the mounting hole.

In addition, the magnetic rack device includes at least one spring member installed on the main body to press the magnetic holder upward, and the rotation member rotated about a hinge axis by moving the magnetic holder downwards. It may further include an operation member for restricting the upward movement of the magnetic holder while compressing the spring member.

In addition, the operation member includes a support coupled to the main body so that at least a portion is located on the upper side of the magnetic fixture, a rotation member rotatably coupled to the support via a hinge shaft, and a predetermined length outward from the rotation member It may include a protruding locking bar, and the magnetic holder may be moved downward by the pressing force provided from the rotation member when the rotation member is rotated.

In addition, the support may further include an insertion groove of a long hole that is formed through to be located on the upper side of the magnetic holder and an insertion groove that is formed by a predetermined depth from one surface to communicate with the placement hole of the long hole and into which the locking bar is inserted, The rotation member may be coupled to the support to be rotated about the hinge shaft within the placement hole.

In addition, the insertion groove may include a first insertion groove and a second insertion groove respectively formed on the left and right both sides of the hinge shaft as a boundary, and through the rotation of the rotating member, the locking bar is inserted into the first insertion groove. When coupled, the permanent magnet may be located at a first position, and when the locking bar is coupled to the second insertion groove, the permanent magnet may be located at a second position relatively lower than the first position.

According to the present invention, the magnetic beads can be smoothly collected by changing the position of the permanent magnet according to the size and shape of the tube so that the permanent magnet is located at a position lower than the storage height of the magnetic bead-containing material contained in the tube. Through this, even if the size or shape of the tube is different, it is possible to easily collect the magnetic beads using a single device, thereby ensuring compatibility.

1 is a view showing a magnetic rack device according to an embodiment of the present invention;
Figure 2 is an exploded view of Figure 1;
3 is a view of FIG. 2 viewed from another direction;
4 is a plan view showing a holder member that can be applied to a magnetic rack device according to an embodiment of the present invention;
5 is a view showing an operating member that can be applied to the magnetic rack device according to an embodiment of the present invention;
6 is a view showing a state in which the holder member is removed from the magnetic rack device according to an embodiment of the present invention;
7 is an operating state diagram showing a state in which the permanent magnet is located in the first position in FIG. 6;
8 is an operation state diagram showing a state in which the permanent magnet is located in the second position in FIG. 6;
9 is a state diagram of a magnetic rack device according to an embodiment of the present invention, and,
10 is another use state diagram of the magnetic rack device according to an embodiment of the present invention.

Hereinafter, with reference to the accompanying drawings, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted, and the same reference numerals are added to the same or similar elements throughout the specification.

The magnetic rack apparatus 100 according to an embodiment of the present invention includes a holder member 120 and a body 110 as shown in FIGS. 1 to 3 .

The holder member 120 may be individually mounted with a plurality of tubes 10 and 20 in which a predetermined amount of a mixture containing a magnetic bead-containing material is stored, respectively.

That is, the tubes 10 and 20 can accommodate a certain amount of a mixture of the magnetic bead-containing material and the nucleic acid and reagent extracted from the sample, and the magnetic bead-containing material contains a certain amount of magnetic beads in a solution such as water or alcohol. may be contained. In addition, the mixture may be used in a diagnostic test using a polymerase chain reaction.

To this end, the holder member 120 may include a plurality of mounting holes 122 to which the tubes 10 and 20 are individually mounted, and the plurality of mounting holes 122 are spaced apart from each other at a predetermined interval. may be formed, and the holder member 120 may be disposed to be positioned on the upper side of the body 110 .

For example, the holder member 120 may be a plate-shaped member having a predetermined area, the plurality of mounting holes 122 may be formed to pass through the plate-shaped member, and the holder member 120 may be At least one fastening bar 150 having a predetermined length may be disposed so as to be positioned on the upper side of the main body 110 o.

Here, the lower end of the fastening bar 150 may be coupled to the main body 110 , more specifically, the pedestal 111 , and the upper end may be fastened to the holder member 120 side.

Through this, the holder member 120 may be detachably coupled to the main body 110 by being separated from the fastening bar 150 or separated from the main body 110 together with the fastening bar 150 .

Through this, when the plurality of tubes 10 and 20 are respectively inserted into the mounting hole 122 , the tubes 10 and 20 may be arranged so that a part of the length is located on the lower side of the holder member 120 . In addition, the side of at least a portion of the portion protruding downward from the holder member 120 by a predetermined length may be disposed to face the permanent magnet 113 to be described later.

In this case, the plurality of mounting holes 122 may be formed to have the same size as each other so that a plurality of tubes 10 and 20 having the same size can be respectively mounted, and the plurality of mounting holes 122 may be formed to have the same size. may be formed on the holder member 120 so as to be arranged in the form of a matrix structure.

For example, the plurality of mounting holes 122 may be formed in the holder member 120 to have a 2×8 matrix structure in the holder member 120 . However, the arrangement relationship and the formation method of the mounting holes 122 are not limited thereto, and an appropriate number may be changed.

At this time, as shown in FIG. 4 , the holder member 120 is the tube 10 and 20 having a relatively larger cross-sectional area than the mounting hole 122 is inserted into the mounting hole 122 side. At least one cutout 123 extending from the mounting hole 122 may be included to allow the (10, 20) to enter the mounting hole 122 side.

That is, when the tubes 10 and 20 having a relatively larger cross-sectional area than the mounting hole 122 are inserted into the mounting hole 122 , the mounting hole 122 is formed by the at least one cutout 123 . can increase the cross-sectional area.

In such a case, the rim portion of the mounting hole 122 in which the cutout 123 is formed moves downward through the pressing force generated while the tubes 10 and 20 are inserted into the mounting hole 122, so that the mounting Tubes 10 and 20 having a relatively larger cross-sectional area than the hole 122 may be inserted into the mounting hole 122 .

Through this, the magnetic rack apparatus 100 according to an embodiment of the present invention uses the cutout 123 even though the plurality of mounting holes 122 formed in the holder member 120 are formed to have the same size as each other. Since the size can be changed, tubes 10 and 20 having various sizes can be mounted in the mounting hole 122 (see FIGS. 9 and 10 ).

For this reason, in the magnetic rack apparatus 100 according to an embodiment of the present invention, even if the sizes and shapes of the tubes 10 and 20 differ depending on the manufacturer, the mounting hole 122 using the cutout 123 is By changing the size, the tubes 10 and 20 of various sizes can be respectively mounted in the mounting hole 122, thereby enhancing compatibility.

The body 110 can support the holder member 120 to which the tubes 10 and 20 are mounted, and at least so that the magnetic beads contained in the mixture of the tubes 10 and 20 can be collected using magnetic force. One permanent magnet 113 may be mounted.

The body 110 may include a pedestal 111 and a magnetic holder 112 coupled to the pedestal 111 , and the permanent magnet 113 may be mounted on the magnetic holder 112 .

Here, the permanent magnets 113 may be provided in plurality to be matched one-to-one with the plurality of tubes 10 and 20 mounted in the mounting hole 122 of the holder member 120 , and the plurality of permanent magnets may be provided. The magnets 113 may be respectively mounted on the magnetic holder 112 at predetermined intervals along one direction.

In addition, when the magnetic holder 112 is formed to have a matrix structure having a plurality of rows and a plurality of columns in the holder member 120 in which the plurality of mounting holes 122 are disposed between two adjacent rows It can be arranged to be positioned.

In this case, the permanent magnet 113 fixed to the mounting portion 112b may be coupled to the magnetic holder 112 so that both ends are exposed to the outside.

Specifically, the magnetic holder 112 includes a coupling portion 112a coupled to the pedestal 111 and a mounting portion 112b extending from the coupling portion 112a so that the plurality of permanent magnets 113 can be mounted. ), and the plurality of permanent magnets 113 may be fixedly installed to the mounting portion 112b at predetermined intervals along the longitudinal direction of the mounting portion 112b.

Through this, when the tubes 10 and 20 are mounted on the side of the holder member 120 , the permanent magnet 113 has one end side of the tubes 10 and 20 mounted on the holder member 120 . When the plurality of mounting holes 122 formed in the holder member 120 are formed to have a matrix structure having a plurality of rows and a plurality of columns, both end sides of the permanent magnet 113 are respectively It can face the side of the tube (10, 20) mounted on the holder member (120).

For this reason, when the tubes 10 and 20 are mounted on the side of the holder member 120 , the end side of the permanent magnet 113 where the magnetic flux is concentrated may face the side of the tubes 10 and 20 . As a result, the magnetic beads contained in the mixture contained in the tubes 10 and 20 can be smoothly collected.

At this time, the magnetic holder 112 may be movably coupled to the pedestal 111 so that the position of the permanent magnet 113 can be changed. That is, the magnetic holder 112 is movable on the pedestal 111 so that the position of the permanent magnet 113 in the lower part of the holder member 120 , and more specifically, the height of the permanent magnet 113 can be changed. can be tightly coupled.

To this end, the pedestal 111 may include a hollow protrusion 111a extending upward from one surface to a predetermined height, and the coupling portion 112a of the magnetic holder 112 is on the protrusion 111a side. can be inserted.

Accordingly, the magnetic holder 112 can move up and down within the protrusion 111a using the coupling portion 112a inserted into the protrusion 111a side, and is mounted on the mounting portion 112b. The mounted permanent magnet 113 may be changed in position (height) by moving the magnetic holder 112 up and down.

In this case, the downward movement of the magnetic holder 112 through the protrusion 111a may be restricted. That is, when the magnetic holder 112 moves downward, the mounting portion 112b may not be inserted into the protrusion 111a.

To this end, as shown in FIG. 3 , the coupling part 112a and the mounting part 112b may be provided to have different thicknesses, and the thickness t2 of the mounting part 112b is the coupling part 112a. ) may be formed to have a relatively thicker thickness than the thickness t1.

In addition, the thickness t1 of the coupling portion 112a may have a thickness that can be inserted into the protrusion 111a, and the thickness t2 of the mounting portion 112b is the thickness t2 of the protrusion 111a. It may be formed to have a size relatively larger than the width.

Accordingly, when the magnetic holder 112 moves downward through the coupling portion 112a, the coupling portion 112a may enter the inside of the protrusion 111a, and the mounting portion 112b is The downward movement may be restricted through the upper edge of the protrusion 111a.

At this time, in the magnetic rack device 100 according to an embodiment of the present invention, at least one spring member ( 130) and an operation member 140 for compressing the spring member 130 by moving the magnetic holder 112 downward may be further included.

That is, the magnetic holder 112 may move upward through the elastic force provided from the spring member 130 . In addition, the magnetic holder 112 moves downward through the operation member 140 and compresses the spring member 130, while upward movement by the elastic force stored in the spring member 130 may be constrained. .

Through this, in the magnetic rack apparatus 100 according to an embodiment of the present invention, the permanent magnet 113 is changed to the first position and the second position through the movement of the magnetic holder 112 in the up and down directions. can be

That is, as shown in FIG. 7 , the magnetic holder 112 moves upward through the pressure of the spring member 130 so that the permanent magnet 113 is changed to the first position or as shown in FIG. 8 . By moving downward through the operation member 140 , the permanent magnet 113 may be changed to a second position relatively lower than the first position.

To this end, the spring member 130 may be disposed on the protrusion 111a so that both ends thereof are in contact with the bottom surfaces of the magnetic holder 112 and the protrusion 111a, respectively, and the operation member 140 is a hinge shaft. At least a portion, including a rotation member 142 that is rotated around 144 , may be coupled to the main body 110 so as to be located on the upper side of the magnetic holder 112 .

That is, as shown in FIGS. 5 and 6 , the operation member 140 includes at least a portion of the support 141 coupled to the pedestal 111 so as to be positioned on the upper side of the magnetic holder 112 , and the hinge It may include a rotation member 142 rotatably coupled to the support 141 via the shaft 144 and a locking bar 143 protruding outward by a predetermined length from the rotation member 142 .

In this case, the rotation member 142 may be rotated about the hinge shaft 144 and come into contact with the magnetic holder 112 to press the magnetic holder 112 downward.

Through this, when the magnetic holder 112 moves downward, the spring member 130 may be compressed as described above.

In this case, the support 141 may include an arrangement hole 145 of a long hole that is formed to be penetrated so as to be positioned on the upper side of the magnetic fixture 112 , and the rotation member 142 is disposed in the arrangement hole 145 . It may be coupled to the support 141 via a hinge shaft 144 to be rotated in the .

Through this, when the rotation member 142 rotates about the hinge shaft 144 in the arrangement hole 145 , the rotation radius of the rotation member 142 may be limited through the locking bar 143 . .

That is, the locking bar 143 may limit the rotation radius of the rotation member 142 by being in contact with the edge defining the arrangement hole 145 when the rotation member 142 is rotated.

In this case, the support 141 may include insertion grooves 146a and 146b into which the locking bar 143 is inserted by being drawn in from one surface to a predetermined depth so as to communicate with the placement hole 145, and the insertion The grooves 146a and 146b may include a first insertion groove 146a and a second insertion groove 146b respectively formed on both left and right sides of the hinge shaft 144 as a boundary.

Accordingly, through the rotation of the rotation member 142, the locking bar 143 may be inserted into the first insertion groove 146a or into the second insertion groove 146b, and the locking bar 143 may be inserted into the second insertion groove 146b. ) is inserted into the first insertion groove (146a) and the second insertion groove (146b), the rotation member 142 may be restricted from rotation in one direction.

Through this, the magnetic holder 112 may maintain the state in which the permanent magnet 113 is located in the first position or the permanent magnet 113 may maintain the state in which the permanent magnet 113 is located in the second position.

For example, as shown in FIG. 7 , when the rotation member 142 rotates so that the locking bar 143 is inserted into the first insertion groove 146a, the magnetic holder 112 is the spring member 130 . ) can be moved upward through the pressing force provided from the Through this, the permanent magnet 113 can be changed to the first position, and the rotation member 142 is limited in rotation through the locking bar 143 inserted into the first insertion groove 146a, so that the permanent magnet 113 is rotated. The magnet 113 may be maintained at the first position.

Conversely, as shown in FIG. 8 , when the rotation member 142 is rotated so that the locking bar 143 is inserted into the second insertion groove 146b, the magnetic holder 112 is the rotation member 142 . It can move downward by the pressing force provided from the rotating member 142 through contact with the . Through this, the spring member 130 may be compressed, the permanent magnet 113 may be changed to a second position, and the rotation member 142 may be caught in the second insertion groove 146b. By restricting rotation through the bar 143 , the permanent magnet 113 may be maintained at the second position.

Accordingly, the magnetic rack device 100 according to an embodiment of the present invention is permanently mounted on the magnetic holder 112 even if the size or shape of the tubes 10 and 20 mounted on the holder member 120 is changed. The position of the magnet 113 may be changed. Through this, even if the storage height (h1, h2) of the mixture containing the magnetic bead-containing material contained in the tube (10, 20) is different depending on the size or shape of the tube (10, 20) of the permanent magnet (113) One end of the tube (10, 20) may face the side of the tube (10, 20) at a position lower than the storage height (h1, h2) of the mixture contained in the tube (10, 20). Through this, it is possible to smoothly collect the magnetic beads M included in the mixture of the tubes 10 and 20 by using the magnetic force generated from the permanent magnet 113 .

For this reason, in the magnetic rack device 100 according to an embodiment of the present invention, the storage height (h1, h2) of the mixture contained in the tube (10, 20) is changed according to the size and shape of the tube (10, 20). By changing the position of the permanent magnet 113 to a position where the magnetic bead (M) can be smoothly collected, even if the size and shape of the tubes (10, 20) are different, the magnetic bead can be easily collected using one device. can Through this, the magnetic rack device 100 of the present invention can be applied to both tubes 10 and 20 having different sizes and shapes, thereby ensuring compatibility.

For example, as shown in FIGS. 9 and 10 , heterogeneous tubes 10 and 20 having different shapes and sizes are respectively mounted on the side of the holder member 120 , and are respectively mounted on the heterogeneous tubes 10 and 20 , respectively. Even if the storage heights (h1, h2) of the mixture contained therein are different, the permanent magnet 113 mounted on the magnetic holder 112 is located below the storage heights (h1, h2) of the mixture contained in the tubes 10 and 20. can be changed.

For this reason, in the magnetic rack device 100 according to an embodiment of the present invention, the storage height (h1, h2) of the mixture contained in the tube (10, 20) is changed according to the size and shape of the tube (10, 20). Even if the magnetic bead (M) can be easily collected by changing the position of the permanent magnet 113 to a position that can smoothly collect the magnetic beads contained in the mixture.

Although one embodiment of the present invention has been described above, the spirit of the present invention is not limited to the embodiments presented herein, and those skilled in the art who understand the spirit of the present invention can add components within the scope of the same spirit. , changes, deletions, additions, etc. may easily suggest other embodiments, but this will also fall within the scope of the present invention.

100: magnetic rack device 110: body
111: pedestal 111a: protrusion
112: magnetic fixture 112a: coupling part
112b: mounting part 113: permanent magnet
120: holder member 122: mounting hole
123: cutout 130: spring member
140: operation member 141: support
142: rotation member 143: catch bar
144: hinge shaft 145: placement hole
146: insertion groove 146a: first insertion groove
146b: second insertion groove 150: fastening bar

Claims (11)

a holder member in which a plurality of mounting holes are formed at predetermined intervals so that a tube in which a mixture containing a magnetic bead-containing material is stored in a predetermined amount can be individually mounted; and
A body including a pedestal and a magnetic holder movably coupled to the pedestal so that a plurality of permanent magnets are mounted at a predetermined interval and the position of the permanent magnet can be changed at a lower position of the holder member magnetic rack unit. According to claim 1,
The holder member is a magnetic rack device in which the lower end is detachably coupled to the main body via at least one fastening bar coupled to the pedestal. According to claim 1,
The holder member includes at least one cutout extending from the mounting hole to allow entry of the tube when a tube having a relatively larger cross-sectional area than the mounting hole is inserted. According to claim 1,
A magnetic rack device in which the plurality of mounting holes are formed to have the same size. According to claim 1,
The pedestal includes a hollow protrusion extending upward from one surface to a certain height,
The magnetic holder includes a coupling portion inserted into the protrusion and a mounting portion to which the plurality of permanent magnets are fixed,
The mounting portion is a magnetic rack device formed to have a relatively larger thickness than the coupling portion so that the downward movement through the upper rim of the protrusion can be constrained. According to claim 1,
The permanent magnet is a magnetic rack device coupled to the magnetic holder so that both ends are exposed to the outside. According to claim 1,
The permanent magnet is a magnetic rack device that is fixed to the magnetic holder so that one end side faces the side of the tube when the tube is inserted into the mounting hole. According to claim 1,
The magnetic rack device includes at least one spring member installed on the main body to press the magnetic holder upward, and the rotation member rotated about a hinge shaft by moving the magnetic holder downward through the rotation of the spring member. Magnetic rack device further comprising an operation member for restricting the upward movement of the magnetic fixture while compressing. 9. The method of claim 8,
The operation member includes a support coupled to the main body so that at least a portion is located on the upper side of the magnetic fixture, a rotation member rotatably coupled to the support via a hinge shaft, and a predetermined length protruding outward from the rotation member including a hook bar;
The magnetic holder is a magnetic rack device that moves downward by the pressing force provided from the rotating member when the rotating member rotates. 10. The method of claim 9,
The support further includes an insertion groove of a long hole that is formed through to be positioned on the upper side of the magnetic holder and an insertion groove that is inserted from one surface to a certain depth so as to communicate with the placement hole of the long hole and into which the locking bar is inserted,
The rotating member is a magnetic rack device coupled to the support so that it can be rotated about the hinge axis in the arrangement hole. 11. The method of claim 10,
The insertion groove includes a first insertion groove and a second insertion groove respectively formed on both left and right sides of the hinge axis as a boundary,
When the locking bar is coupled to the first insertion groove through rotation of the rotation member, the permanent magnet is positioned at a first position, and when the locking bar is coupled to the second insertion groove, the permanent magnet is more A magnetic rack device positioned at a relatively low second position.

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