KR20220033568A - Microorganism for promoting the immunity of Newcastle disease vaccine - Google Patents

Abstract

The present invention relates to a composition for enhancing immunity of a chicken Newcastle disease vaccine, and when Pseudomcenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas caeni JLRI 03, and Rhodobacter spheroides JLRI 04 are treated, a chicken Newcastle disease vaccine antibody formation rate is increased in laying hens and broilers, and at the same time it is confirmed that an odor reduction effect is also excellent, thereby being able to be used to enhance vaccine immunity and reduce odor of chickens.

Description

Microorganism for promoting the immunity of Newcastle disease vaccine

The present invention relates to a composition for enhancing immunity of a chicken Newcastle disease vaccine and to a use thereof.

Newcastle disease is a deadly and highly contagious first-class livestock infectious disease in poultry, with a 100% mortality rate when infected with unvaccinated chickens, respiratory and digestive symptoms, and egg laying rates in laying hens if not properly vaccinated. It is a fatal disease that causes economic damage due to deterioration. Laying hens or breeders with low vaccine antibody titers may experience reduced or even discontinuation of egg production if appropriate vaccines are not given. In addition, even if vaccinated, the timing or method of inoculation is wrong, and in chickens that do not have high antibody levels, neurological symptoms such as paralysis of the legs and neck may appear.

Korea is an area where Newcastle disease virus (NDV) is common, and although an outbreak warning is issued every year, the incidence continues to increase and is occurring nationwide, causing great damage to poultry farms. In addition, since various Newcastle disease viruses are prevalent in Southeast Asia, China, and Taiwan, which are actively trading with Korea, they exist as potential harmful factors, so the development of a Newcastle disease vaccine is urgently needed.

In order to improve the productivity of poultry, the development of related drugs such as various growth promoters is being made steadily. When the drug is continuously used, the number of resistant microorganisms increases and the efficacy thereof decreases, or due to problems such as remaining in livestock products, the current situation in Europe and the like is refraining from using it as much as possible. As an alternative to this, research is being actively conducted to develop a probiotic that inhibits the growth of harmful bacteria in the intestine and has the effect of promoting the growth of poultry, improving productivity, and reducing odor. Probiotics play a role in preventing imbalances in the intestinal microflora that may be caused by various stresses, such as known live microorganism feed additives that have a beneficial effect on the host by improving the balance of the intestinal microflora.

As a result of repeated research, the present inventors have identified microorganisms that enhance the immune effect of the chicken Newcastle disease vaccine and completed the present invention.

Republic of Korea Patent No. 10-1200235

The problem to be solved by the present invention is a composition for enhancing immunity of chicken Newcastle disease vaccine including Leukonostok pseudomesenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strains And to provide a method for enhancing immunity of chicken Newcastle disease vaccine using the same.

In order to solve the above problems, the present invention provides at least one selected from the group consisting of Leukonostok Pseudomescenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strains. It provides a composition for enhancing immunity of chicken Newcastle disease vaccine comprising a.

The composition may be used for odor reduction at the same time.

It also provides a feed for enhancing immunity of chicken Newcastle disease vaccine comprising the composition.

It also provides a feed additive for enhancing immunity of chicken Newcastle disease vaccine comprising the composition.

In addition, the present invention provides a step of adding a first microbial agent comprising Leuconostok Pseudomesenteroides JLRI 01 to feed or beverage, and Bacillus thuringiensis JLRI 02, Pseudomonas caenie JLRI 03 and Rhodobacter spheroides JLRI 04 It provides a method for enhancing immunity of chicken Newcastle disease vaccine comprising the step of spraying a microbial agent containing one or more selected from the strain.

The second microorganism preparation may contain 1×10 ⁶ cfu/ml to 1×10 ⁸ cfu/ml to microorganisms.

The second microbial agent may be sprayed to the chicken house at intervals of 1 to 5 days.

The present invention relates to a composition for enhancing immunity of a chicken Newcastle disease vaccine, and when treated with Leukonostok Pseudomescenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaenie JLRI 03 and Rhodobacter spheroides JLRI 04 strains, chicken Newcastle disease It increases the vaccine antibody formation rate of the vaccine and at the same time reduces odor substances and has an excellent odor reduction effect.

1 is a result of analyzing the odor reduction effect of microbial treatment in a broiler house in Farmhouse 1 (Yeonggwang).
2 is a result of analyzing the odor reduction effect according to the microbial treatment in the broiler house of Farmhouse 2 (Muan).
3 is a result of analyzing the vaccine antibody formation rate and titer of broilers according to microbial treatment in Farmhouse 1 (Yeongwang).
4 is a result of analyzing the vaccine antibody formation rate and titer of broilers according to microbial treatment in Farmhouse 2 (Muan).
5 is a result of analyzing the odor reduction effect according to the microbial treatment in the laying hen house of Farmhouse 3 (Muan).
6 is a result of analyzing the odor reduction effect according to the microbial treatment in the laying hen house of Farmhouse 4 (Muan).
7 is a result of analyzing the vaccine antibody formation rate and titer of laying hens according to microbial treatment in Farmhouse 3 (Muan).
8 is a result of analyzing the vaccine antibody formation rate and titer of laying hens according to microbial treatment in Farmhouse 4 (Muan).

The present invention is a chicken Newcastle disease vaccine comprising at least one selected from the group consisting of Leukonostok Pseudomcentroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strains. It provides a composition for enhancing immunity.

The composition for enhancing immunity of the present invention contains at least one selected from the group consisting of Leukonostok Pseudomesenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strains. do.

In the present invention, Leuconostoc pseudomesenteroides JLRI 01 ( Leuconostoc pseudomesenteroides JLRI 01) is a microorganism with accession number KCCM 11443P of the Korea Microbial Conservation Center.

In the present invention, Bacillus thuringiensis JLRI 02 ( Bacillus thuringiensis JLRI 02) is a microorganism of the Korea Microorganism Conservation Center accession number KCCM 11490P.

In the present invention, Pseudomonas caeni JLRI 03 ( Pseudomonas caeni JLRI 03) is a microorganism of the Korea Microorganism Conservation Center accession number KCCM 11491P.

In the present invention, Rhodobacter sphaeroides JLRI 04 is a microorganism of the Korea Microorganism Conservation Center accession number KCCM 11492P.

In the present invention, immune enhancement is meant to enhance the protective effect against disease, specifically, means to increase the antibody formation rate of the chicken Newcastle disease vaccine by microbial treatment.

In the present invention, chicken Newcastle disease is a kind of deadly and highly contagious first-class livestock infectious disease in poultry, and when infected with unvaccinated chickens, causes 100% mortality, It is a fatal disease that causes economic damage due to digestive symptoms and a decrease in the laying rate in laying hens. If an appropriate vaccine is not administered, laying hens or breeders with low vaccine antibody titers have a problem in that the laying rate is reduced or stopped.

The composition for enhancing immunity may be added to feed additives including feed or drinking water, or it may be used for spraying livestock.

In an embodiment of the present invention, treatment of the microorganisms of the Leukonostok Pseudomcenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strain microorganisms ND vaccine antibody formation rate in broilers and laying hens It was confirmed that this increased, and at the same time it was confirmed that the odor substance was reduced, Leukonostok Pseudomcenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaenie JLRI 03 and Rhodobacter spheroides JLRI 04 strain microorganisms Newcastle Vaccine It can be used to enhance immunity.

In addition, it was confirmed that the odor reduction effect during the microbial treatment was confirmed, and the composition may be used for reducing odor at the same time.

In the present invention, the odor reduction means reducing ammonia or amines.

In an embodiment of the present invention, treatment of microorganisms with Leukonostok Pseudomesenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strain microorganisms in broilers and laying hens in an embodiment of the present invention for broilers and laying hens As it was confirmed that it does not affect the productivity, the composition for enhancing immunity of the present invention does not reduce the productivity of broilers and laying hens, and may exhibit vaccine immunity enhancing effects.

It also provides a feed for enhancing immunity of chicken Newcastle disease vaccine comprising the composition.

The feed of the present invention contains one or more selected from the group consisting of Leukonostok Pseudomcenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strains, including at least one selected from chicken Newcastle disease It is characterized by increasing the rate of antibody formation against the vaccine.

The feed of the present invention can enhance the immune effect of the Newcastle disease vaccine in chickens to enhance resistance to the chicken Newcastle disease, an infectious disease, and reduce the mortality rate.

In the above-mentioned examples, it was confirmed that the treatment of Leukonostok Pseudomesenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 reduced the odorous substances in the cage, The feed of the present invention can exhibit the effect of reducing the odor of the cage.

It also provides a feed additive for enhancing immunity of chicken Newcastle disease vaccine comprising the composition.

The feed additive of the present invention is a product intended to be ingested as a supplement to a normal diet in order to overcome the lack of daily intake of a specific compound, or an antibiotic and a substance for improving immunity that are ingested to improve disease persistence, In the present invention, it refers to a substance added to feed to enhance the immune effect of chickens.

The feed additive of the present invention may further include a material used as a conventional feed additive other than the composition.

In the above-described embodiment of the present invention, Leuconostok Pseudomesenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 Microbial treatment of the Newcastle disease vaccine of chickens without reducing the productivity of the chickens To improve the antibody formation rate, the feed additive of the present invention increases the immunity of chickens, and specifically, it is characterized in that it has an immune enhancing effect against Newcastle disease.

In addition, the present invention is a step of adding a first microbial preparation comprising Leukonostok Pseudomcenteroides JLRI 01 to feed or beverage and Bacillus thuringiensis JLRI 02, Pseudomonas caenie JLRI 03 and Rhodobacter spheroides JLRI 04 strains It provides a method for enhancing immunity of chicken Newcastle disease vaccine comprising the step of spraying a microbial agent comprising at least one selected from.

In the present invention, the descriptions of Leukonostok Pseudomenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaenie JLRI 03 and Rhodobacter spheroides JLRI 04 are the same as described above.

In an embodiment of the present invention, by selecting broilers and laying hens, Leuconostoc pseudomesenteroides JLRI 01 for feed was added to feed or negative water at 0.2 wt%, and Bacillus thuringiensis JLRI 02 for spraying, Pseudomonas caeni JLRI 03 and Rhodobacter sphaeroides JLRI 04) were added to 0.2L/ It was sprayed twice a week in m 2 , and the treated strain was treated with a microorganism preparation at 1×10 ⁶ cfu/ml (treatment 1) and 1×10 ⁸ cfu/ml (treatment 2).

In the present invention, the cfu represents a colony forming unit.

Therefore, the step of adding of the present invention is to add the first microbial preparation comprising Leukonostok Pseudomescenteroides JLRI 01 to feed or negative water, specifically 1×10 ⁶ cfu/ml to 1×10 ⁸ cfu/ It may be added in a ratio of 0.01 wt% to 10 wt% of the first microbial preparation containing the microbes in ml.

The spraying step of the present invention is a second microbial preparation comprising at least one of Bacillus thuringiensis JLRI 02, Pseudomonas caenie JLRI 03 and Rhodobacter spheroides JLRI 04 with respect to the chicken house using a liquid or It can be sprayed directly in powder form, and in general, the second microorganism preparation in which the three kinds of microorganisms are mixed can be sprayed by diluting an appropriate multiple, and in the case of a cage, the microorganism preparation in which the three kinds of microorganisms are mixed is 1 During the week, it can be sprayed inside the house every two days.

Specifically, the second microbial preparation may contain microorganisms in a range of 1×10 ⁶ cfu/ml to 1×10 ⁸ cfu/ml to.

The spraying step may be spraying the second microbial agent to the chicken house at intervals of 1 to 5 days.

In the above-mentioned examples, in broilers and laying hens, Leukonostok Pseudomesenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas caenie JLRI 03 and Rhodobacter spheroides JLRI 04 strain microorganisms treated with odorant ammonia, As it was confirmed that the amines were reduced, the immune enhancing method of the present invention may exhibit a odor reduction effect at the same time.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

<Materials and Methods>

Broiler and laying hen farms were selected as shown in Table 1, and the laboratory's own developed strains ( Leuconostoc pseudomesenteroides JLRI 01 for feed, Bacillus thuringiensis JLRI 02 for spraying, Pseudomonas caeni JLRI 03 and Rhodobacter sphaeroides JLRI 04) were treated without treatment and 1 (1×10) ⁶ cfu/ml), treatment 2 (1×10 ⁸ cfu/ml) was performed as shown in Table 2 by placing three test plots.

Table 1 shows the status of test farms for broilers and laying hens.

Table 2 shows the contents of microbial treatment. Referring to Table 2, leukonostok pseudomesenteroides were added in 0.2 wt% to feed or drinking water, and Bacillus thuringiensis, Pseudomonas caenie, and Rhodobacter spheroids were sprayed at 0.2 L/m ² . In addition, by varying the treatment concentration of the strain, treatment group 1 (1×10 ⁶ cfu/ml) and treatment group 2 (1×10 ⁸ cfu/ml) were treated with microorganisms in the test group.

Body weight was measured at the time of insertion and weekly, and the total weight for each repetition was weighed and divided by the number of individuals to determine the average weight. For feed intake, the cumulative feed intake for each week was investigated. The growth rate was expressed as a percentage by dividing the number of harvests by the number of shipments and then multiplying by 100. The production index was calculated by dividing the growth rate multiplied by the average body weight by the feed demand rate multiplied by the number of shipping days, and multiplied by 100.

Average weight = total weight / number of individuals

Feed demand rate = feed intake/weight gain

Growth rate (%) = (number of shipments / number of harvests) × 100

Production index = {(average weight × breeding rate)/(feed demand rate × number of days shipped)} × 100

For broilers, from 3 weeks of age to the time of shipment, and for laying hens from 1 week after the start of the experiment to 8 weeks after the experiment is finished, ammonia (3La) and amines (180) are collected in the central corridor of the breeding building using a malodorous gas detector (Gastec, Japan). The odor reduction effect was analyzed by measuring once a week and three times a week.

In addition, in order to examine whether the antibody titer to the vaccine has risen, Newcastle disease (ND), infectious bronchitis (IB) is NDV/IBV ELISA kit (Median Diagnostics, Republic of Korea), and infectious bursitis (IBD) is IBDV ab test kit ( IDEXX, USA) was used.

<Results and considerations>

Culture of strains and evaluation of contamination level

Table 3 and Table 4 show the results of evaluation of the number of viable cells and contamination after mass-culturing the test strain to be applied to the farm at 35 to 37° C. in an aerobic condition for 3 to 5 days.

In Table 3, the number of viable strains applied to broiler farms is Leukonostok Pseudomesenteroides JLRI 01 strain 7.8×10 ¹⁰ cfu/ml, Bacillus thuringiensis JLRI 02 strain 6.1×10 ⁹ cfu/ml, Pseudomonas caenie JLRI 03 strain 2.3×10 ⁹ cfu/ml and Rhodobacter spheroides JLRI 04 strain 1.6×10 ⁹ cfu/ml were confirmed, and it was confirmed that it was not contaminated with pathogenic microorganisms and molds such as E. coli and Salmonella. Table 3 shows the number of viable microorganisms and the degree of contamination of broiler farms.

In addition, in Table 4, the number of viable strains applied to the laying hen farms is Leukonostok Pseudomescenteroides JLRI 01 strain 6.7×10 ¹⁰ cfu/ml, Bacillus thuringiensis JLRI 02 strain 5.1×10 ⁹ cfu/ml, Pseudomonas Kaeni JLRI 03 It was confirmed that the strain was 3.8×10 ⁹ cfu/ml and Rhodobacter spheroides JLRI 04 strain 2.9×10 ⁹ cfu/ml, and it was confirmed that it was not contaminated with pathogenic microorganisms and molds such as E. coli and Salmonella. Table 4 shows the number of viable microorganisms and the degree of contamination of the laying hen farms.

The effect of improving the productivity of broilers

Table 5 shows the results of the investigation of broiler productivity (farm 1, farm 2). In Farmhouse 2, the production index improved by 4% (454.9 → 472.9) due to an increase in weight gain by 2% (1,806g → 1832.8g) in the 2nd treatment group (high concentration, 1×10 ⁸ ). there was no Table 5 shows the broiler productivity according to the concentration of microorganisms applied.

Reduction of odor substances in broiler farms

The results of investigating the effect of reducing odor substances in broiler farms are shown in FIGS. 1 and 2 . In 2 groups (1×10 ⁸ ) treated compared to the control group, farmhouse 1 (Yeonggwang) reduced ammonia by 60% (4ppm → 2.5ppm) and amines by 64% (5ppm → 1.8ppm), and farmhouse 2 (Muan) reduced ammonia by 34% ( 5ppm → 3.3ppm), amines were reduced by 37% (10ppm → 6.3ppm). Amines are generated when a part of the feed consumed by livestock (protein source) is not digested but excreted as feces and decomposed by anaerobes. is considered to be the cause.

Vaccine immunity boosting effect of broilers

Vaccine antibody results for each broiler disease are shown in FIGS. 3 and 4. This can be seen as a result of vaccination according to the vaccination program, and in two farms, Newcastle disease (ND), Infectious bronchitis virus (IBV), or Infectious bursal disease virus, Vaccine antibody formation rate and vaccine antibody titer against IBDV) were compared.

As a result of the analysis, it was confirmed that the ND vaccine antibody formation rate was increased in both farmhouse 1 and farmhouse 2 when microorganisms were treated. For the IBV vaccine, the vaccine antibody formation rate was 100% in the control group in both farms, and the vaccine antibody formation rate was 100% after treatment with microorganisms, so the immune enhancing effect of the vaccine by the microorganisms could not be confirmed. .

In the case of IBDV, it was confirmed that the vaccine antibody formation rate was significantly increased in the 2 treated groups (1×10 ⁸ ) compared to the control in Farmhouse 1 (Yeongwang), but in Farmhouse 2 (Muan), the vaccine antibody formation rate was significantly increased in the 2 treated groups (1×10 ⁸ ). It was confirmed that the antibody formation rate was reduced.

There was no significant difference in the ND vaccine antibody titers in both farms 1 and 2 (p>0.05), but the IB vaccine antibody titers increased in the 2 treated groups (1×108) compared to the control in Farm 1 (Yeongwang) ( 4.9 → 5.6) (p<0.05), and in farmhouse 2 (Muan), it was higher in the control group than in the treatment groups 1 and 2 (6.3/6.6 → 7.3) (p<0.05).

That is, it was confirmed that the treatment of Leukonostok Pseudomenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strain microorganisms increases the ND vaccine antibody formation rate in broilers. At the same time, it was confirmed that the odor substances were reduced. Leukonostok Pseudomcenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strain microorganisms for the purpose of enhancing immunity in Newcastle vaccine can be used

In addition, it may be characterized in that the odor reduction effect is exhibited at the same time as the microorganism treatment.

In addition, in broiler farms, the treatment of microorganisms with strains of Leukonostok Pseudomcenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas caeni JLRI 03 and Rhodobacter spheroides JLRI 04 increases or affects the productivity of broilers. As it was confirmed that it did not affect , shows the effect of enhancing vaccine immunity.

Analysis of impact on laying hen productivity

Table 6 shows the results of investigating changes in the productivity of laying hens for the treatment with microorganisms, and there was no difference in feed intake, feed requirement, egg laying rate, and egg weight in both farms 3 and 4 according to the concentration of microorganisms. That is, it was confirmed that the microbial treatment did not affect the productivity of the laying hens.

Odor reduction effect of laying hens

The results of investigation of the odorant reduction effect in the laying hen farms of the treatment with microorganisms are shown in FIGS. 5 and 6 . When both farms of farmhouse 3 and farmhouse ⁴ were applied at high concentrations, odor substances were reduced. → 14.3) decreased, and in farmhouse 4, ammonia 43% (7.5ppm → 4.3) and amines decreased by 33% (18ppm → 12.1).

Vaccine immunity boosting effect in laying hens

Newcastle disease (ND), chicken infectious bronchitis virus (IBV), chicken pneumovirus (Avian Pneumovirus, AP) vaccine antibodies of laying hens are shown in Figures 7 and 8. It was detected in all treatment groups of both farms 3 and 4, which can be seen as a result of performing the vaccine according to the vaccination program, and the vaccine antibody formation rate and vaccine antibody titer by disease were compared.

As a result of the analysis, it was confirmed that the ND vaccine antibody formation rate was increased in both farmhouse 3 and farmhouse 3 when microorganisms were treated. For the IBV vaccine, the vaccine antibody formation rate was 100% in the control group in both farms, and the vaccine antibody formation rate was 100% after treatment with microorganisms, so the immune enhancing effect of the vaccine by the microorganisms could not be confirmed. . In the case of AP, it was confirmed that the vaccine antibody formation rate decreased in the 2 treatment groups (1×10 ⁸ ) compared to the control group in farmhouse 3, but in farmhouse 4, the vaccine antibody formation rate increased in the 2 treatment groups (1×10 ⁸ ) in farmhouse 4 Confirmed.

The ND vaccine antibody titer increased by 13% (4.6 → 5.3) in the 2 treated groups (high concentration, 1×10 ⁸ ) compared to the control in farmhouse 4, and the IB vaccine antibody titer was the highest in the control group in farmhouse 3 (6.3/7.3 → 7.7).

That is, it was confirmed that the treatment of Leukonostok Pseudomesenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strain microorganisms increased the ND vaccine antibody formation rate in laying hens. At the same time, it was confirmed that the odor substances were reduced. Leukonostok Pseudomcenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strain microorganisms for the purpose of enhancing immunity in Newcastle vaccine can be used In addition, it may be characterized in that the odor reduction effect is exhibited at the same time as the microorganism treatment.

In addition, it was confirmed that the treatment of Leukonostok pseudomesenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strain microorganisms in the laying hen farms did not affect the viability of the laying hens. As said, the treatment of the Leukonostok Pseudomenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas Kaeni JLRI 03 and Rhodobacter spheroides JLRI 04 strain microorganisms does not reduce the productivity of laying hens, and enhances vaccine immunity. show the effect.

Korea Microorganism Conservation Center (Overseas) KCCM11443P 20130814 Korea Microorganism Conservation Center (Overseas) KCCM11490P 20131127 Korea Microorganism Conservation Center (Overseas) KCCM11491P 20131127 Korea Microorganism Conservation Center (Overseas) KCCM11492P 20131127

Claims (7)

For immune enhancement of chicken Newcastle disease vaccine comprising at least one selected from the group consisting of Leukonostok Pseudomenteroides JLRI 01, Bacillus thuringiensis JLRI 02, Pseudomonas caenie JLRI 03 and Rhodobacter spheroides JLRI 04 strain composition. The method of claim 1,
The composition is for the purpose of reducing odor at the same time, the composition. A feed for enhancing immunity of a chicken Newcastle disease vaccine comprising the composition of claim 1 or 2. A feed additive for enhancing immunity of a chicken Newcastle disease vaccine comprising the composition of claim 1 or 2. adding a first microbial agent comprising leukonostok pseudomesenteroides JLRI 01 to the feed or beverage; and
Bacillus thuringiensis JLRI 02, Pseudomonas Kaenie JLRI 03 and Rhodobacter spheroids JLRI 04 A method of enhancing immunity of a chicken Newcastle disease vaccine comprising the step of spraying a microbial agent comprising at least one selected from the strain. 6. The method of claim 5,
Wherein the second microbial preparation comprises 1 × 10 ⁶ cfu / ml to 1 × 10 ⁸ cfu / ml to microorganisms,
How to boost immunity. 6. The method of claim 5,
The second microbial agent is to be sprayed to the cage at intervals of 1 to 5 days,
How to boost immunity.

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