KR20220033093A - Portable real-time gene analysis system with improved reaction efficiency, and Grinding unit therefor - Google Patents

Portable real-time gene analysis system with improved reaction efficiency, and Grinding unit therefor Download PDF

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KR20220033093A
KR20220033093A KR1020200114932A KR20200114932A KR20220033093A KR 20220033093 A KR20220033093 A KR 20220033093A KR 1020200114932 A KR1020200114932 A KR 1020200114932A KR 20200114932 A KR20200114932 A KR 20200114932A KR 20220033093 A KR20220033093 A KR 20220033093A
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gene analysis
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time
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gene
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박성식
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바이오뱅크 주식회사
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Abstract

The present invention relates to a portable real-time gene analysis system having improved reaction efficiency and a grinding unit used therefor. The gene analysis system according to the present invention includes: a grinding unit (10); a gene extractor (30); and a real-time gene analyzer (40). The real-time gene analysis system according to the present invention is provided as a single system integrated with the grinding unit, and thus reduces the time required for reaction with a reagent, time required for amplification and the time required for reading the analysis result. In this manner, it is possible to reduce the time required for a series of processes, including extracting DNA gene substances promptly from a test sample, separating multiple specific genes at once and reading the result from the separated images, thereby allowing rapid analysis in the field.

Description

반응효율이 향상된 휴대용 실시간 유전자 분석시스템 및 이에 사용되는 그라인딩유닛{Portable real-time gene analysis system with improved reaction efficiency, and Grinding unit therefor}Portable real-time gene analysis system with improved reaction efficiency, and grinding unit therefor used therefor

본 발명은 실시간 유전자 분석시스템에 관한 것으로, 보다 상세하게는 그라인딩 유닛을 이용하여 추출된 샘플의 세포벽을 파쇄함으로써, 시약과의 핵산 추출반응을 높임으로써 실시간으로 DNA분석이 필요한 장소에서 손쉽게 간편하게 직접 이를 수행할 수 있도록 구성된 반응효율이 향상된 휴대용 실시간 유전자 분석시스템 및 이에 사용되는 그라인딩유닛에 관한 것이다.The present invention relates to a real-time gene analysis system, and more particularly, by breaking the cell wall of the extracted sample using a grinding unit, thereby increasing the nucleic acid extraction reaction with the reagent, it can be easily and conveniently directly carried out at a place requiring real-time DNA analysis. It relates to a portable real-time gene analysis system with improved reaction efficiency configured to perform and a grinding unit used therein.

일반적으로, 대상체로부터 채취한 DNA의 분석은 중합 효소 연쇄반응(PCR) 방법이 광범위하게 사용되고 있다. 이와 같은 PCR 방법을 진행하기 위해서는 채취한 DNA를 증폭할 수 있는 써멀 싸이클러(thermal cycler) 장비와 채취한 DNA의 분석을 제어하는 제어 장비가 필요하게 된다.In general, a polymerase chain reaction (PCR) method is widely used for analysis of DNA collected from a subject. In order to proceed with such a PCR method, a thermal cycler equipment capable of amplifying the collected DNA and a control equipment controlling the analysis of the collected DNA are required.

그러나, 종래의 PCR 방법이 적용된 실시간 PCR 장비는 데스크톱용으로 개발되어 크기가 매우 크고, 데이터 처리를 위하여 외부의 컴퓨터와 연결되어 동작하도록 구성되어 있으며, 내부 구조는 공간적 효율성이 낮게 설계되어 있다. 따라서, 실제 현장에서 사용하기에는 비효율적이고 불필요한 부분이 많다고 할 수 있다.However, the real-time PCR equipment to which the conventional PCR method is applied is developed for desktop and has a very large size, is configured to operate in connection with an external computer for data processing, and the internal structure is designed to have low spatial efficiency. Therefore, it can be said that there are many parts that are inefficient and unnecessary to use in the actual field.

특히, 구제역과 같은 유전성 전염병을 판정하기 위해서 구제역 의심 동물의 DNA를 분석하고자 할 경우 또는 이 DNA를 실시간 PCR 장비가 설치된 장소로 이동하여 분석해야 하므로, 해당 지역을 이탈하게 되어 2차 전염이 발생될 우려가 있을 뿐만 아니라, 이동 시간에 따른 방역 관리도 어려운 심각한 문제점이 발생될 수 있다.In particular, if you want to analyze the DNA of an animal suspected of foot-and-mouth disease in order to determine a hereditary infectious disease such as foot-and-mouth disease, or you need to move the DNA to a place where real-time PCR equipment is installed and analyze it, you may leave the area and cause secondary transmission. Not only is there a concern, but serious problems may arise that make it difficult to manage quarantine according to travel time.

또한, 감염체의 유전물질과 같은 직접 인자의 존재유무를 확인하는 것으로 조기진단이 가능한 검사법인 분자생물학적 검사는, 검사자 또는 오염에 의한 거짓 양성 결과가 도출되는 위험이 있다.In addition, molecular biological tests, which are tests capable of early diagnosis by confirming the presence or absence of direct factors such as genetic material of infectious agents, have a risk of deriving false positive results due to testers or contamination.

이에 따라, 최근에는 WHO에서 감염성 질환 진단을 위한 현장검사의 필요성을 역설하고 있고, 대략 10여 년 전부터 분자생물학적 방법을 이용한 현장 검사용 시스템은 꾸준히 개발이 진행되고 있다.Accordingly, the WHO has recently emphasized the necessity of a point-of-care test for diagnosing infectious diseases, and a system for point-of-care testing using a molecular biological method has been steadily developed since about 10 years ago.

더욱이, 일반적으로 검체로부터 유전물질의 추출, 유전자증폭 반응, 증폭 유무의 진단 등으로 이루어지는 일체의 분자진단 절차를 하나의 시스템에서 이루어지도록 하는 랩온어칩(LOC) 기술의 출현에 따라, 현장진단이 가능한 고효율, 고감도의 시스템에 대한 연구진행이 더욱 활발히 진행되고 있다.Furthermore, with the advent of lab-on-a-chip (LOC) technology, which allows all molecular diagnostic procedures, such as extraction of genetic material from a specimen, gene amplification reaction, and diagnosis of amplification presence or absence, to be performed in one system, on-site diagnosis has become Research on the possible high-efficiency and high-sensitivity system is being conducted more actively.

이와 같은 연구결과물로, 한국등록특허 제10-1767978호에 공개된 분자진단 현장검사 장치용 스트립센서모듈, 한국등록특허 제10-1302353호에 공개된 유전자 정량 검출 실시간 PCR 및 유전자 분석 DNA 마이크로어레이를 복합적으로 수행할 수 있는 분자진단 자동분석기기 등이 있다.As a result of such research, a strip sensor module for molecular diagnostics on-site inspection device disclosed in Korean Patent No. 10-1767978 and a real-time PCR and gene analysis DNA microarray disclosed in Korean Patent No. 10-1302353 were developed. There are molecular diagnostic automatic analysis devices that can perform complex tasks.

그러나, 이와 같은 종래의 분자진단 현장검사 장치의 경우, 일반적으로 DNA칩 및 측정장치 구성이 복잡하여 제품제조 단가비용이 높아 1회 테스트 사용비용도 높은 단점이 있고, PCR생성물의 결과를 확인하기 위해서는 별도의 측정장치로 옮겨야 하므로 옮기는 과정에서의 오염에 의한 측정신뢰도가 낮아지는 단점이 있으며, 유전자 추출, 증폭, 분리, 판독이 하나의 시스템내에서 이루어지지 않아 초기 판정은 어느 정도 가능하지만 최종 결과를 얻기까지 많은 시간이 소요되어 현장검사에 적합하지 않은 단점이 있다.However, in the case of such a conventional molecular diagnostic on-site inspection device, in general, the configuration of the DNA chip and measuring device is complicated, and the product manufacturing cost is high, so the cost of using one test is high. Since it has to be moved to a separate measuring device, there is a disadvantage in that the measurement reliability is lowered due to contamination during the transfer process. It takes a lot of time to obtain, so it has the disadvantage that it is not suitable for on-site inspection.

더욱이, 현재 현장검사용으로 Real time PCR이 많이 개발되고 있으나, 유전자 추출에 많은 시간이 소요되어 현장검사에 적합하지 않은 단점도 있고, 우리나라의 경우 현실상 기술적인 면에서 선진국가와 차이가 나고 고가 장비와 시약으로 인하여 후발주자로서의 한계에 직면하고 있다.Moreover, although many real-time PCRs are currently being developed for on-site inspection, there is a disadvantage that it takes a lot of time for gene extraction and is not suitable for on-site inspection. Due to reagents, we are facing limitations as a latecomer.

또한, 핵산을 이용한 분자진단법은 높은 정확성과 재현성, 신속성 등의 장점이 있어 최근 각 연구분야에서 많은 이슈가 되고 있는 방법이나, 구현하는 데 있어 가장 큰 장애물은 DNA를 추출하기 위한 세포 파괴(cell lysis) 공정의 집적화이다.In addition, molecular diagnostics using nucleic acids has advantages such as high accuracy, reproducibility, and speed, which is a method that has recently become a hot topic in each research field, but the biggest obstacle to implementation is cell lysis (cell lysis) for extracting DNA. ) is the integration of the process.

최근 세포 파괴를 위한 여러 집적 기술이 개발되어 효율성 및 경제성이 개선되었으나, 정밀한 온도 제어가 필요한 화학적 세포 파괴 방법에 비해 구현이 훨씬 수월하고 세포벽 파쇄가 신속하게 가능하여 반응효율을 높일 수 있는 물리적 세포 파괴를 이용한 방법에 대한 필요성이 요구되고 있다.Recently, several integration technologies for cell destruction have been developed, improving efficiency and economic feasibility, but compared to chemical cell destruction methods that require precise temperature control, implementation is much easier and cell wall disruption is possible quickly, resulting in physical cell destruction that can increase reaction efficiency There is a need for a method using

한국등록특허 제10-1767978호Korean Patent Registration No. 10-1767978 한국등록특허 제10-1302353호Korean Patent Registration No. 10-1302353

본 발명은 상술한 문제점을 해결하기 위해 안출된 것으로서, 본 발명의 목적은 그라인딩유닛과 일체화된 하나의 장치시스템을 통해 검체로부터 DNA 유전자물질을 신속하게 추출하고, 이를 증폭시켜 다수개의 특이 유전자를 한번에 분리하고, 분리된 이미지로부터 결과를 판독하는 일련의 과정을 현장에서 신속하게 실시간으로 수행할 수 있도록 구성된 반응효율이 향상된 휴대용 실시간 유전자 분석시스템 및 이에 사용되는 그라인딩유닛을 제공하는 것이다.The present invention has been devised to solve the above problems, and an object of the present invention is to rapidly extract DNA genetic material from a specimen through a single device system integrated with a grinding unit, and amplify it to generate a plurality of specific genes at once. An object of the present invention is to provide a portable real-time gene analysis system with improved reaction efficiency and a grinding unit used therein, configured to perform a series of processes of separating and reading results from the separated images quickly and in real time in the field.

상술한 문제를 해결하기 위하여, 본 발명에 따른 반응효율이 향상된 휴대용 실시간 유전자 분석시스템은,In order to solve the above problems, the portable real-time gene analysis system with improved reaction efficiency according to the present invention,

그라인딩유닛(10); 유전자 추출기(30); 및 실시간 유전자 분석기(40);를 포함하여 구성되는 것을 특징으로 한다.grinding unit 10; gene extractor 30; And a real-time gene analyzer 40; characterized in that it is configured to include.

또한, 본 발명에 따른 휴대용 실시간 유전자 분석시스템에서,In addition, in the portable real-time gene analysis system according to the present invention,

상기 그라인딩유닛(10)은, The grinding unit 10,

회동지지대(11); 상기 회동지지대(11)의 일단에 결합되는 회동체(12); 및 상기 회동체의 전단에 결합되며, 상기 회동체(12)와 함께 회동하는 파쇄봉(15);을 포함하여 구성되는 것을 특징으로 한다.rotation support (11); a rotating body 12 coupled to one end of the rotating support 11; and a crushing rod (15) coupled to the front end of the rotating body and rotating together with the rotating body (12).

또한, 본 발명에 따른 휴대용 실시간 유전자 분석시스템에서,In addition, in the portable real-time gene analysis system according to the present invention,

상기 회동지지대(11)에는 모터가 내장되어 인가되는 전력에 의해 모터의 회전이 제어되며,The rotation support 11 has a built-in motor, and the rotation of the motor is controlled by the applied power,

상기 회동체(12)는 후단은 상기 회동지지대(11)의 일단을 통해 모터의 샤프트에 연결되어 모터의 제어에 따라 회동이 제어되고, 전단에는 상기 파쇄봉(15)의 타단이 결합되는 결합홀(13)이 형성되며,The rear end of the rotating body 12 is connected to the shaft of the motor through one end of the rotation support 11 so that rotation is controlled according to the control of the motor, and the front end has a coupling hole to which the other end of the crushing rod 15 is coupled. (13) is formed,

상기 파쇄봉(15)은 후단에는 상기 결합홀(13)에 결합되는 결합부(15a)가 형성되고, 전단에는 테이퍼형상의 파쇄부(15b)가 형성되는 것을 특징으로 한다.The crushing rod 15 is characterized in that a coupling portion 15a coupled to the coupling hole 13 is formed at the rear end, and a tapered crushing portion 15b is formed at the front end.

또한, 본 발명에 따른 휴대용 실시간 유전자 분석시스템에서,In addition, in the portable real-time gene analysis system according to the present invention,

상기 유전자 추출기(30)는, 다수개의 시약이 구비된 시약부(31)와 주사기(32) 및 다수개의 마이크로튜브(20)를 포함하여 구성되어, 세포벽 또는 세포막이 파쇄된 혈액검체에 시약을 투입하여 반응을 일으키도록 하는 것을 특징으로 한다.The gene extractor 30 includes a reagent unit 31 provided with a plurality of reagents, a syringe 32 and a plurality of microtubes 20, and injects reagents into a blood sample in which cell walls or cell membranes are disrupted. It is characterized in that it causes a reaction.

또한, 본 발명에 따른 휴대용 실시간 유전자 분석시스템에서,In addition, in the portable real-time gene analysis system according to the present invention,

상기 실시간 유전자 분석기(40)는, 원심분리부(41)와 유전자분석부(42) 및 디스플레이부(43)로 구성되어, 시약이 분주된 다수개의 마이크로튜브(20)로부터 실시간으로 유전자분석을 수행하고 그 결과를 표시하는 것을 특징으로 한다.The real-time gene analyzer 40 is composed of a centrifuge unit 41, a gene analysis unit 42, and a display unit 43, and performs gene analysis in real time from a plurality of microtubes 20 in which reagents are dispensed. and displaying the result.

또한, 본 발명에 따른 휴대용 실시간 유전자 분석시스템에서,In addition, in the portable real-time gene analysis system according to the present invention,

상기 유전자분석부(42)에는, 혈청의 항원-항체반응을 측정하여 암이나 바이러스를 진단하고 검사를 수행하도록, 분리된 혈청만이 공급되는 구조를 구비하거나The gene analysis unit 42 has a structure in which only the separated serum is supplied to diagnose cancer or a virus by measuring the antigen-antibody reaction of the serum and perform the test, or

반응후 이미지 캡쳐나 흡광도 측정이 용이하도록 카메라 및 조명을 구비하는 것을 특징으로 한다.It is characterized in that it is equipped with a camera and lighting to facilitate image capture or absorbance measurement after the reaction.

또한, 본 발명에 따른 휴대용 실시간 유전자 분석시스템에서,In addition, in the portable real-time gene analysis system according to the present invention,

상기 디스플레이부(43)는, 상기 유전자분석부(42)로부터 전송받은 흡광도, 캡쳐된 이미지 자료 및 분석된 정보나 저장된 기준자료와 비교한 자료를 디스플레이하는 것을 특징으로 한다.The display unit 43 is characterized in that it displays the absorbance received from the gene analysis unit 42, captured image data, and analyzed information or data compared with stored reference data.

또한, 본 발명에 따른 휴대용 실시간 유전자 분석시스템에 사용되는 그라인딩유닛은,In addition, the grinding unit used in the portable real-time gene analysis system according to the present invention,

회동지지대(11); 상기 회동지지대(11)의 일단에 결합되는 회동체(12); 및 rotation support (11); a rotating body 12 coupled to one end of the rotating support 11; and

상기 회동체의 전단에 결합되며, 상기 회동체(12)와 함께 회동하는 파쇄봉(15);을 포함하여 구성되며, 마이크로튜브내에 투입된 혈액검체의 세포벽이나 세포막을 파쇄시키는 것을 특징으로 한다.It is coupled to the front end of the rotating body and is configured to include;

또한, 본 발명에 따른 그라인딩유닛은,In addition, the grinding unit according to the present invention,

상기 회동지지대(11)에는 모터가 내장되어 인가되는 전력에 의해 모터의 회전이 제어되며,The rotation support 11 has a built-in motor, and the rotation of the motor is controlled by the applied power,

상기 회동체(12)는 후단은 상기 회동지지대(11)의 일단을 통해 모터의 샤프트에 연결되어 모터의 제어에 따라 회동이 제어되고, 전단에는 상기 파쇄봉(15)의 타단이 결합되는 결합홀(13)이 형성되며,The rear end of the rotating body 12 is connected to the shaft of the motor through one end of the rotation support 11 so that rotation is controlled according to the control of the motor, and the front end has a coupling hole to which the other end of the crushing rod 15 is coupled. (13) is formed,

상기 파쇄봉(15)은 후단에는 상기 결합홀(13)에 결합되는 결합부(15a)가 형성되고, 전단에는 테이퍼형상의 파쇄부(15b)가 형성되는 것을 특징으로 한다.The crushing rod 15 is characterized in that a coupling portion 15a coupled to the coupling hole 13 is formed at the rear end, and a tapered crushing portion 15b is formed at the front end.

또한, 본 발명에 따른 휴대용 실시간 유전자 분석방법은,In addition, the portable real-time gene analysis method according to the present invention,

채취된 혈액검체를 마이크로튜브(20)에 투입한 후, 그라인딩유닛(10)의 회동하는 파쇄봉(15)을 이용하여 검체의 세포벽 또는 세포막을 파쇄시키는 세포벽 파쇄단계(S1);After putting the collected blood sample into the microtube 20, the cell wall crushing step (S1) of crushing the cell wall or membrane of the sample using the crushing rod 15 that rotates of the grinding unit 10;

다수개의 마이크로튜브(20)의 파쇄된 혈액샘플에 시약을 공급, 염색시약처리하여 핵산이 감별되도록 하는 DNA/RNA 추출단계(S2); 및A DNA/RNA extraction step (S2) of supplying reagents to the shredded blood samples of a plurality of microtubes 20 and treating with a dyeing reagent to discriminate nucleic acids; and

시약이 분주된 다수개의 마이크로튜브(20)를 원심분리부(41)에 투입하여, 혈액샘플을 적혈구(RBC), 백혈구(WBC)와 혈소판(Platelet), 혈장으로 분획하고, 유전자분석부(42)에서 혈청의 항원-항체반응을 측정하여 암이나 바이러스를 진단하고 분석하며, 분석된 정보나 저장된 기준자료와 비교한 자료는 디스플레이부(43)에 디스플레이하는 실시간 유전자 분석단계(S3);를 포함하여 구성되는 것을 특징으로 한다.A plurality of microtubes 20 in which reagents are dispensed are put into the centrifuge unit 41, and the blood sample is fractionated into red blood cells (RBC), white blood cells (WBC), platelets, and plasma, and the gene analysis unit 42 ) by measuring the antigen-antibody reaction of the serum to diagnose and analyze cancer or viruses, and a real-time gene analysis step (S3) of displaying the analyzed information or data compared with the stored reference data on the display unit 43; includes; It is characterized in that it is configured.

본 발명에 따른 반응효율이 향상된 휴대용 실시간 유전자 분석시스템은, 그라인딩유닛과 일체화된 하나의 장치시스템을 통해 검체로부터 DNA 유전자물질을 신속하게 추출하고, 이를 증폭시켜 다수개의 특이 유전자를 한번에 분리하고, 분리된 이미지로부터 결과를 판독하는 일련의 과정의 시간을 절감시켜 현장에서 신속하게 수행할 수 있는 효과가 있다.The portable real-time gene analysis system with improved reaction efficiency according to the present invention rapidly extracts DNA genetic material from a specimen through a single device system integrated with a grinding unit, and amplifies it to isolate and separate multiple specific genes at once It has the effect of being able to quickly perform in the field by reducing the time of the series of processes of reading the results from the image.

또한, 본 발명에 따른 그라인딩유닛은, 순식간에 세포벽 파쇄가 가능하도록 구성되어, 시약반응소요시간, 증폭소요시간 및 분석판독까지의 시간을 절감시키는 효과가 있다.In addition, the grinding unit according to the present invention is configured to break the cell wall in an instant, and has the effect of reducing the time required for reagent reaction, time required for amplification, and time required for analysis and reading.

도 1은 본 발명의 일실시예에 따른 휴대용 실시간 유전자 분석시스템의 전체구성을 간략하게 보여주는 도면.
도 2는 도 1의 그라인딩유닛의 결합관계를 나타내는 도면.
도 3은 도 1의 그라인딩유닛의 작동상태를 나타내는 도면.
도 4는 본 발명의 일실시예에 따른 휴대용 실시간 유전자 분석시스템의 공정단계를 간략하게 보여주는 도면.1 is a diagram schematically showing the overall configuration of a portable real-time gene analysis system according to an embodiment of the present invention.
FIG. 2 is a view showing a coupling relationship of the grinding unit of FIG. 1; FIG.
Fig. 3 is a view showing an operating state of the grinding unit of Fig. 1;
4 is a diagram schematically showing the process steps of a portable real-time gene analysis system according to an embodiment of the present invention.

이하, 첨부된 도면을 참조하여 본 발명인 휴대용 실시간 유전자 분석시스템의 바람직한 실시예를 더욱 상세하게 설명한다.Hereinafter, a preferred embodiment of the present invention's portable real-time gene analysis system will be described in more detail with reference to the accompanying drawings.

도 1은 본 발명의 일실시예에 따른 휴대용 실시간 유전자 분석시스템의 전체구성을 간략하게 보여주는 도면, 도 2는 도 1의 그라인딩유닛의 결합관계를 나타내는 도면, 도 3은 도 1의 그라인딩유닛의 작동상태를 나타내는 도면, 도 4는 본 발명의 일실시예에 따른 휴대용 실시간 유전자 분석시스템의 수행단계를 간략하게 보여주는 도면이다.1 is a diagram schematically showing the overall configuration of a portable real-time gene analysis system according to an embodiment of the present invention, FIG. 2 is a diagram showing the coupling relationship of the grinding unit of FIG. 1, and FIG. 3 is the operation of the grinding unit of FIG. 4 is a diagram schematically showing the steps of performing a portable real-time gene analysis system according to an embodiment of the present invention.

도 1 내지 도 4에 도시된 바와 같이, 본 발명에 따른 반응효율이 향상된 휴대용 실시간 유전자 분석시스템(100)은 그라인딩유닛(10), 유전자 추출기(30) 및 실시간 유전자 분석기(40)를 포함하여 구성된다.1 to 4, the portable real-time gene analysis system 100 with improved reaction efficiency according to the present invention includes a grinding unit 10, a gene extractor 30, and a real-time gene analyzer 40. do.

여기서, 그라인딩유닛(10)은, 추출된 혈액 검체의 세포벽을 물리적인 방법으로 신속하게 파쇄시키는 것으로, 회동지지대(11), 회동지지대(11)의 일단에 탈착식으로 끼워 결합되는 회동체(12), 회동체의 전단에 탈착식으로 끼워 결합되며, 회동체(12)와 함께 회동하는 파쇄봉(15)을 포함하여 구성된다.Here, the grinding unit 10 is to rapidly crush the cell wall of the extracted blood sample by a physical method, and the rotational support 11, the rotational support 11, which is detachably fitted to one end of the rotational support 11, is coupled to the rotational body 12. , is coupled detachably to the front end of the rotating body, and is configured to include a crushing rod 15 that rotates together with the rotating body (12).

이때, 회동지지대(11)에는 모터(미도시)가 내장되어 인가되는 전력에 의해 모터의 회전이 제어될 수 있다. 또한, 회동체(12)는 후단은 회동지지대(11)의 일단을 통해 모터의 샤프트에 연결되어 모터의 제어에 따라 회동이 제어되며, 전단에는 파쇄봉의 타단이 끼워 결합되는 결합홀(13)이 형성되어 있다.At this time, the rotation support 11 has a motor (not shown) built in, the rotation of the motor can be controlled by the applied power. In addition, the rear end of the rotating body 12 is connected to the shaft of the motor through one end of the rotation support 11 so that the rotation is controlled according to the control of the motor, and the front end has a coupling hole 13 through which the other end of the crushing rod is fitted and coupled. is formed

또한, 파쇄봉(15)은 소정 길이를 가진 봉형상으로 후단에는 결합홀(13)에 끼워 맞춤결합되는 결합부(15a)가 형성되어 있고, 전단에는 테이퍼형상의 파쇄부(15b)가 형성되어 있고, 중간 소정위치에는 넘침방지부가 형성되어 있다. 파쇄부(15b)의 형상은 마이크로튜브(20)의 하부 내면 형상에 대응되는 형상으로, 이는 투입된 검체(P)의 파쇄를 용이하게 하기 위함이다.In addition, the crushing rod 15 has a rod shape having a predetermined length, at the rear end is formed a coupling portion 15a to be fitted into the coupling hole 13, and a tapered crushing portion 15b is formed at the front end, and an overflow prevention part is formed at a predetermined intermediate position. The shape of the crushing part 15b is a shape corresponding to the shape of the lower inner surface of the microtube 20, which is to facilitate crushing of the injected specimen P.

유전자 추출기(30)는 세포벽 또는 세포막이 파쇄된 혈액검체에 시약을 투입하여 반응을 일으키는 장치로서, 다수개의 시약이 구비된 시약부(31)와 주사기(32) 및 다수개의 마이크로튜브(20)를 포함하여 구성된다.The gene extractor 30 is a device that causes a reaction by injecting a reagent into a blood sample whose cell wall or cell membrane is disrupted. A reagent unit 31 equipped with a plurality of reagents, a syringe 32 and a plurality of microtubes 20 is comprised of

시약부(31)에는 측정하고자 하는 검사항목에 맞는 다수개의 지시약이 구비되어, 이를 마이크로튜브의 파쇄된 혈액샘플에 공급, 염색시약처리하여 5가지 백혈구를 염색하여 염색된 캡쳐 이미지로부터 백혈구를 감별, 계수할 수 있도록 한다. 다수개의 마이크로튜브(20)에는 세포벽 또는 세포막이 파쇄된 혈액검체가 구비되어 있어, 1번에 다량의 검사가 가능하도록 한다.The reagent unit 31 is provided with a plurality of indicators suitable for the test item to be measured, supplied to the shredded blood sample of the microtube, treated with a dyeing reagent to stain 5 types of white blood cells, and differentiated white blood cells from the captured images, to be able to count. A plurality of microtubes 20 are provided with a blood sample in which the cell wall or cell membrane is disrupted, so that a large amount of examination is possible at one time.

다수개의 마이크로튜브(20)에는 주사기(32)를 통해 시약부(31)에서 측정항목에 맞는 시약이 각각 분주된다.Reagents corresponding to the measurement items in the reagent unit 31 are dispensed into the plurality of microtubes 20 through the syringe 32 , respectively.

실시간 유전자 분석기(40)는, 시약이 분주된 다수개의 마이크로튜브(20)로부터 실시간으로 유전자분석을 수행하고 그 결과를 표시하는 것으로, 원심분리부(41)와 유전자분석부(42) 및 디스플레이부(43)로 구성된다.The real-time gene analyzer 40 performs gene analysis in real time from a plurality of microtubes 20 in which reagents are dispensed and displays the results, and includes a centrifuge unit 41, a gene analysis unit 42 and a display unit. It consists of (43).

시약이 분주된 다수개의 마이크로튜브(20)를 원심분리부(41)에 투입하고 소정시간 동안 소정 rpm으로 회전시키게 되면, 혈액샘플은 원심력에 의해 원심분리되어 적혈구(RBC), 백혈구(WBC)와 혈소판(Platelet), 혈장으로 분획된다. When a plurality of microtubes 20 in which reagents are dispensed are put into the centrifuge unit 41 and rotated at a predetermined rpm for a predetermined time, the blood sample is centrifuged by centrifugal force to produce red blood cells (RBC), white blood cells (WBC) and It is fractionated into platelets and plasma.

이를 유전자분석부(42)에 투입하게 되면, 분리된 이미지를 캡쳐하여 구간별 길이 등을 측정하여 용적량으로 전체 %를 산출함으로써 적혈구, 백혈구와 혈소판 등의 혈구계수를 계산한다. 이를 통해 혈액샘플의 적혈구나 백혈구 등의 숫자 및 변형 정도가 측정 분석된다.When this is input to the gene analysis unit 42, the blood cell count of red blood cells, white blood cells, platelets, etc. is calculated by capturing the separated images, measuring the length of each section, and calculating the total % by volume. Through this, the number and degree of deformation of red blood cells or white blood cells in the blood sample are measured and analyzed.

이때, 유전자분석부(42)에는, 혈청의 항원-항체반응을 측정하여 암이나 바이러스를 진단하고 검사를 수행하도록, 분리된 혈청만이 공급되는 구조(미도시)를 구비할 수 있다.In this case, the gene analysis unit 42 may have a structure (not shown) in which only the separated serum is supplied so as to diagnose cancer or a virus by measuring the antigen-antibody reaction of the serum and perform the test.

유전자분석부(42)에는 반응후 이미지 캡쳐나 흡광도 측정이 용이하도록 카메라 및 조명을 구비할 수 있다.The gene analysis unit 42 may be provided with a camera and lighting to facilitate image capture or absorbance measurement after the reaction.

디스플레이부(43)는, 유전자분석부(42)로부터 전송받은 흡광도, 캡쳐된 이미지 자료 및 분석된 정보나 저장된 기준자료와 비교한 자료를 디스플레이한다. The display unit 43 displays the absorbance received from the gene analysis unit 42, captured image data, and data compared with analyzed information or stored reference data.

이하, 본 발명에 따른 휴대용 실시간 유전자 분석시스템의 공정단계를 간략하게 설명하면 다음과 같다.Hereinafter, the process steps of the portable real-time gene analysis system according to the present invention will be briefly described as follows.

(S1) 세포벽 파쇄단계(S1) cell wall disruption step

채취된 혈액검체를 마이크로튜브(20)에 투입한 후, 그라인딩유닛(10)의 회동하는 파쇄봉(15)을 이용하여 신속하게 검체의 세포벽 또는 세포막을 파쇄시킨다. After the collected blood sample is put into the microtube 20, the cell wall or membrane of the sample is rapidly crushed using the crushing rod 15 that rotates of the grinding unit 10.

(S2) DNA/RNA 추출단계(S2) DNA/RNA extraction step

마이크로튜브(20)의 파쇄된 혈액샘플에 검사항목에 맞는 다수개의 지시약을 공급, 염색시약처리하여 핵산이 감별되도록 한다.Nucleic acids are discriminated by supplying a plurality of indicators suitable for test items to the shredded blood sample of the microtube 20 and treating them with a dyeing reagent.

(S3) 실시간 유전자 분석단계(S3) Real-time gene analysis step

시약이 분주된 다수개의 마이크로튜브(20)를 원심분리부(41)에 투입하여, 혈액샘플이 적혈구(RBC), 백혈구(WBC)와 혈소판(Platelet), 혈장으로 분획되도록 한다.A plurality of microtubes 20 in which reagents are dispensed are put into the centrifuge unit 41 so that the blood sample is fractionated into red blood cells (RBC), white blood cells (WBC), platelets (Platelet), and plasma.

그럼 다음, 유전자분석부(42)에서 혈청의 항원-항체반응을 측정하여 암이나 바이러스를 진단하고 분석한다.Then, the gene analysis unit 42 measures the antigen-antibody reaction of the serum to diagnose and analyze cancer or viruses.

분석된 정보나 저장된 기준자료와 비교한 자료는 디스플레이부(43)에 디스플레이한다. The analyzed information or data compared with the stored reference data is displayed on the display unit 43 .

본 발명의 실시간 유전자 분석시스템에 따르면, 그라인딩유닛과 일체화된 하나의 장치시스템으로 구성되어, 시약반응소요시간, 증폭소요시간 및 분석판독까지의 시간을 절감시켜, 검체로부터 DNA 유전자물질을 신속하게 추출이 가능하며, 이로 인하여 증폭 및 다수개의 특이 유전자를 한번에 분리하는 것이 가능하고, 분리된 이미지로부터 결과를 판독하는 일련의 과정의 시간을 절감시켜 현장에서 신속하게 수행할 수 있게 된다.According to the real-time gene analysis system of the present invention, it is composed of a single device system integrated with the grinding unit, and the reagent reaction time, amplification time, and time until analysis and reading are reduced, and DNA genetic material is rapidly extracted from the sample. This is possible, whereby it is possible to amplify and isolate a plurality of specific genes at once, and it is possible to quickly perform in the field by reducing the time of a series of processes of reading the results from the separated images.

또한, 간편하고 신속하게 검사가 가능하면서도 장비의 간소화되어 휴대가 용이하므로 현장에서 실시가 신속하고 간편하게 이루어질 수 있다.In addition, simple and quick inspection is possible, and since the equipment is simplified and easy to carry, it can be carried out quickly and easily in the field.

본 발명은 도면을 참조하여 실시예를 참고로 설명하였으나, 이는 예시적인 것에 불과하며, 당해 분야에서 통상의 지식을 가진자라면 이로부터 다양한 변형 및 균등한 실시예가 가능하다는 점을 이해할 것이다. 따라서, 본 발명의 진정한 기술적 보호범위는 첨부된 특허청구범위의 기술적 사상에 의해 정해져야 할 것이다.Although the present invention has been described with reference to the embodiments with reference to the drawings, it will be understood that these are merely exemplary, and that various modifications and equivalent embodiments are possible therefrom by those skilled in the art. Accordingly, the true technical protection scope of the present invention should be defined by the technical spirit of the appended claims.

100: 휴대용 실시간 유전자 분석시스템
10: 그라인딩유닛 11: 회동지지대
12: 회동체 13: 결합홀
15: 파쇄봉
15a: 결합부 15b: 파쇄부
20: 마이크로튜브
30: 유전자 추출기 31: 시약부
32: 주사기
40: 실시간 유전자 분석기
41: 원심분리부 42: 유전자분석부
43: 디스플레이부100: portable real-time gene analysis system
10: grinding unit 11: rotation support
12: rotating body 13: coupling hole
15: crushing rod
15a: coupling part 15b: crushing part
20: microtube
30: gene extractor 31: reagent unit
32: syringe
40: Real-time genetic analyzer
41: centrifugation unit 42: gene analysis unit
43: display unit

Claims (10)

그라인딩유닛(10);
유전자 추출기(30); 및
실시간 유전자 분석기(40);를 포함하여 구성되는 것을 특징으로 하는 반응효율이 향상된 휴대용 실시간 유전자 분석시스템.
grinding unit 10;
gene extractor 30; and
A portable real-time gene analysis system with improved reaction efficiency, characterized in that it comprises a real-time gene analyzer (40).
 제1 항에 있어서,
상기 그라인딩유닛(10)은,
회동지지대(11); 상기 회동지지대(11)의 일단에 결합되는 회동체(12); 및 상기 회동체의 전단에 결합되며, 상기 회동체(12)와 함께 회동하는 파쇄봉(15);을 포함하여 구성되는 것을 특징으로 하는 반응효율이 향상된 휴대용 실시간 유전자 분석시스템.
According to claim 1,
The grinding unit 10,
rotation support (11); a rotating body 12 coupled to one end of the rotating support 11; and a crushing rod (15) coupled to the front end of the rotating body and rotating together with the rotating body (12).
 제2 항에 있어서,
상기 회동지지대(11)에는 모터가 내장되어 인가되는 전력에 의해 모터의 회전이 제어되며,
상기 회동체(12)는 후단은 상기 회동지지대(11)의 일단을 통해 모터의 샤프트에 연결되어 모터의 제어에 따라 회동이 제어되고, 전단에는 상기 파쇄봉(15)의 타단이 결합되는 결합홀(13)이 형성되며,
상기 파쇄봉(15)은 후단에는 상기 결합홀(13)에 결합되는 결합부(15a)가 형성되고, 전단에는 테이퍼형상의 파쇄부(15b)가 형성되는 것을 특징으로 하는 반응효율이 향상된 휴대용 실시간 유전자 분석시스템.
3. The method of claim 2,
The rotation support 11 has a built-in motor, and the rotation of the motor is controlled by the applied power,
The rear end of the rotating body 12 is connected to the shaft of the motor through one end of the rotation support 11 so that rotation is controlled according to the control of the motor, and the front end has a coupling hole to which the other end of the crushing rod 15 is coupled. (13) is formed,
The crushing rod 15 is a portable real-time with improved reaction efficiency, characterized in that a coupling portion 15a coupled to the coupling hole 13 is formed at the rear end, and a tapered crushing portion 15b is formed at the front end. Genetic analysis system.
 제1 항에 있어서,
상기 유전자 추출기(30)는, 다수개의 시약이 구비된 시약부(31)와 주사기(32) 및 다수개의 마이크로튜브(20)를 포함하여 구성되어, 세포벽 또는 세포막이 파쇄된 혈액검체에 시약을 투입하여 반응을 일으키도록 하는 것을 특징으로 하는 반응효율이 향상된 휴대용 실시간 유전자 분석시스템.
According to claim 1,
The gene extractor 30 includes a reagent unit 31 provided with a plurality of reagents, a syringe 32 and a plurality of microtubes 20, and injects reagents into a blood sample in which cell walls or membranes are disrupted. A portable real-time gene analysis system with improved reaction efficiency, characterized in that it causes a reaction.
 제1 항에 있어서,
상기 실시간 유전자 분석기(40)는, 원심분리부(41)와 유전자분석부(42) 및 디스플레이부(43)로 구성되어, 시약이 분주된 다수개의 마이크로튜브(20)로부터 실시간으로 유전자분석을 수행하고 그 결과를 표시하는 것을 특징으로 하는 반응효율이 향상된 휴대용 실시간 유전자 분석시스템.
According to claim 1,
The real-time gene analyzer 40 is composed of a centrifuge unit 41, a gene analysis unit 42, and a display unit 43, and performs gene analysis in real time from a plurality of microtubes 20 in which reagents are dispensed. and a portable real-time gene analysis system with improved reaction efficiency, characterized in that it displays the result.
 제5 항에 있어서,
상기 유전자분석부(42)에는, 혈청의 항원-항체반응을 측정하여 암이나 바이러스를 진단하고 검사를 수행하도록, 분리된 혈청만이 공급되는 구조를 구비하거나
반응후 이미지 캡쳐나 흡광도 측정이 용이하도록 카메라 및 조명을 구비하는 것을 특징으로 하는 반응효율이 향상된 휴대용 실시간 유전자 분석시스템.
6. The method of claim 5,
The gene analysis unit 42 has a structure in which only the separated serum is supplied to diagnose cancer or a virus by measuring the antigen-antibody reaction of the serum and perform the test, or
A portable real-time gene analysis system with improved reaction efficiency, characterized in that it is equipped with a camera and lighting for easy image capture or absorbance measurement after reaction.
 제5 항에 있어서,
상기 디스플레이부(43)는, 상기 유전자분석부(42)로부터 전송받은 흡광도, 캡쳐된 이미지 자료 및 분석된 정보나 저장된 기준자료와 비교한 자료를 디스플레이하는 것을 특징으로 하는 반응효율이 향상된 휴대용 실시간 유전자 분석시스템.
6. The method of claim 5,
The display unit 43 is a portable real-time gene with improved reaction efficiency, characterized in that it displays the absorbance received from the gene analysis unit 42, captured image data, and analyzed information or data compared with the stored reference data. analysis system.
 회동지지대(11);
상기 회동지지대(11)의 일단에 결합되는 회동체(12); 및
상기 회동체의 전단에 결합되며, 상기 회동체(12)와 함께 회동하는 파쇄봉(15);을 포함하여 구성되며,
마이크로튜브내에 투입된 혈액검체의 세포벽이나 세포막을 파쇄시키는 것을 특징으로 하는 그라인딩유닛.
rotation support (11);
a rotating body 12 coupled to one end of the rotating support 11; and
It is coupled to the front end of the rotating body, and the crushing rod 15 that rotates together with the rotating body 12; is configured to include,
A grinding unit characterized in that it crushes the cell wall or the cell membrane of the blood sample injected into the microtube.
 제8 항에 있어서,
상기 회동지지대(11)에는 모터가 내장되어 인가되는 전력에 의해 모터의 회전이 제어되며,
상기 회동체(12)는 후단은 상기 회동지지대(11)의 일단을 통해 모터의 샤프트에 연결되어 모터의 제어에 따라 회동이 제어되고, 전단에는 상기 파쇄봉(15)의 타단이 결합되는 결합홀(13)이 형성되며,
상기 파쇄봉(15)은 후단에는 상기 결합홀(13)에 결합되는 결합부(15a)가 형성되고, 전단에는 테이퍼형상의 파쇄부(15b)가 형성되는 것을 특징으로 하는 그라인딩유닛.
9. The method of claim 8,
The rotation support 11 has a built-in motor, and the rotation of the motor is controlled by the applied power,
The rear end of the rotating body 12 is connected to the shaft of the motor through one end of the rotation support 11 so that rotation is controlled according to the control of the motor, and the front end has a coupling hole to which the other end of the crushing rod 15 is coupled. (13) is formed,
The crushing rod (15) is a grinding unit, characterized in that a coupling portion (15a) coupled to the coupling hole (13) is formed at the rear end, and a tapered crushing portion (15b) is formed at the front end.
 채취된 혈액검체를 마이크로튜브(20)에 투입한 후, 그라인딩유닛(10)의 회동하는 파쇄봉(15)을 이용하여 검체의 세포벽 또는 세포막을 파쇄시키는 세포벽 파쇄단계(S1);
다수개의 마이크로튜브(20)의 파쇄된 혈액샘플에 시약을 공급, 염색시약처리하여 핵산이 감별되도록 하는 DNA/RNA 추출단계(S2); 및
시약이 분주된 다수개의 마이크로튜브(20)를 원심분리부(41)에 투입하여, 혈액샘플을 적혈구(RBC), 백혈구(WBC)와 혈소판(Platelet), 혈장으로 분획하고, 유전자분석부(42)에서 혈청의 항원-항체반응을 측정하여 암이나 바이러스를 진단하고 분석하며, 분석된 정보나 저장된 기준자료와 비교한 자료는 디스플레이부(43)에 디스플레이하는 실시간 유전자 분석단계(S3);를 포함하여 구성되는 것을 특징으로 하는 반응효율이 향상된 휴대용 실시간 유전자 분석방법.After putting the collected blood sample into the microtube 20, the cell wall crushing step (S1) of crushing the cell wall or the cell membrane of the sample using the crushing rod 15 that rotates of the grinding unit 10;
A DNA/RNA extraction step (S2) of supplying reagents to the shredded blood samples of a plurality of microtubes 20 and treating with a dyeing reagent to differentiate nucleic acids; and
A plurality of microtubes 20 in which reagents are dispensed are put into the centrifuge unit 41, and the blood sample is fractionated into red blood cells (RBC), white blood cells (WBC), platelets, and plasma, and the gene analysis unit 42 ) by measuring the antigen-antibody reaction of the serum to diagnose and analyze cancer or viruses, and a real-time gene analysis step (S3) of displaying the analyzed information or data compared with the stored reference data on the display unit 43; includes; A portable real-time gene analysis method with improved reaction efficiency, characterized in that it is constructed by
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