KR20220028314A - A preparation method of Peanut sprout extract with increased content of nucleobase - Google Patents

Abstract

Provided are a method for producing a peanut sprout extract having an increased nucleobase content, a peanut sprout extract manufactured by the manufacturing method, a pharmaceutical composition for treating or preventing osteoporosis comprising the same, and a health functional food for preventing or alleviating osteoporosis comprising the same. The present invention can provide an optimal extraction method capable of extracting a physiologically active substance effective for the treatment, prevention or alleviation of osteoporosis from peanut sprouts with a low cost and a simple process.

Description

Method for producing peanut sprout extract with increased nucleobase content {A preparation method of Peanut sprout extract with increased content of nucleobase}

The present invention relates to a method for producing a peanut sprout extract having an increased nucleobase content.

With the recent advances in medicine and the improvement of living standards, the average lifespan of human beings is increasing, and the world has already entered an aging society. The incidence of diseases highly associated with Therefore, health management has become more important, and modern people are paying much attention not only to the treatment of diseases but also to the prevention of diseases through functional foods. As a material for functional food, there is a growing tendency to prefer natural-derived materials that have stability and physiological effects on the human body.

Peanuts are an annual herbaceous plant in the genus of beans and peanuts, and when germination from peanuts into peanut sprouts, they biosynthesize beneficial nutrients to protect them from the external environment. Peanut sprouts are known to have anti-inflammatory, antibacterial and antioxidant properties, and to help prevent various diseases such as cardiovascular disease, cancer, and Alzheimer's. As such, peanut sprouts are a medicinal food material, and various products have been produced due to their excellent physiological effects, whereas research on efficient peanut extraction methods is insufficient.

The present inventors have completed the present invention at the end of an effort to develop a method for producing a peanut extract having an increased nucleobase content at a low cost in consideration of the material characteristics of the peanut sprout.

Republic of Korea Patent Publication No. 10-2018-0133997

An object of the present invention is to provide a method for preparing a peanut sprout extract having an increased nucleobase content.

In addition, an object of the present invention is to provide a peanut sprout extract prepared by the above method, a pharmaceutical composition for treating or preventing osteoporosis comprising the extract, and a health functional food for preventing or improving osteoporosis.

One aspect provides a method for producing a peanut sprout extract with increased nucleobase content.

Another aspect provides a peanut sprout extract prepared by the above manufacturing method, a pharmaceutical composition for treating or preventing osteoporosis using the same, and a health functional food for preventing or improving osteoporosis.

In one aspect, it was confirmed that the manufacturing method can increase the nucleobase content, which is an active ingredient for bone health, from the peanut sprout extract, and increase the total content of uracil and adenine, and peanuts with low cost and simple process It is possible to provide an optimal extraction method capable of extracting a physiologically active substance effective for the treatment, prevention or improvement of osteoporosis from the sprout.

In addition, the extract prepared according to this can be used as a pharmaceutical or health functional food for the treatment, prevention, or improvement of osteoporosis, and since it uses an edible natural product-derived extract as its active ingredient, there is no problem with side effects or cytotoxicity There are advantages. In addition, the extract can be used as a dairy product, food and beverage, or health functional food showing a remarkable effect in preventing or improving osteoporosis.

Figure 1 (a) shows the HPLC chromatogram of uracil (Uracil) and adenine (Adenine) contained in the peanut sprout extract according to the present invention. 1(b) and 1(c) show calibration curves prepared using uracil and adenine as standard materials, respectively.
Figures 2 (a) to (d) are, respectively, the concentration % (v / v) of the ethanol extraction solvent, the concentration % (v / v) of the isopropanol extraction solvent, the pH of the ethanol extraction solvent, or peanuts according to the germination period of peanut sprouts The yield (%) (w/w) of the sprout extract is shown.
Figure 3 shows the total content (㎍ / g) of uracil and adenine obtained from the peanut sprout extract according to the concentration % (v / v) of the ethanol extraction solvent.
4 shows the total content (㎍/g) of uracil and adenine obtained from the peanut sprout extract according to the concentration % (v/v) of the isopropanol extraction solvent.
Figure 5 shows the total content (㎍ / g) of uracil and adenine obtained from the peanut sprout extract according to the pH of the extraction solvent.
Figure 6 shows the total content (㎍ / g) of uracil and adenine obtained from the peanut sprout extract according to the germination period (growth day) of the peanut sprout.
Figure 7 (a) shows the total content (㎍ / g) of uracil and adenine obtained by extracting peanut sprouts 10 days after germination with 30% ethanol or 30% isopropanol adjusted to pH 4, 5, or 7.
7(b) shows the total content (㎍/g) of uracil and adenine obtained by extracting peanut sprouts 14 days after germination with 30% ethanol or 30% isopropanol adjusted to pH 4, 5, or 7.
Figure 7 (c) shows the total content (㎍ / g) of uracil and adenine obtained by extracting peanut sprouts 17 days after germination with 30% ethanol or 30% isopropanol adjusted to pH 4, 5, or 7.

Hereinafter, the present invention will be described in more detail.

All technical terms used in the present invention, unless otherwise defined, have the meanings commonly understood by one of ordinary skill in the art of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention. The contents of all publications herein incorporated by reference are hereby incorporated by reference in their entirety.

One aspect of the present invention is

Extracting the peanut sprout with an extraction solvent to obtain a peanut sprout extract,

The extraction solvent comprises an alcohol having 1 to 3 carbon atoms, characterized in that the pH is 4 to 5,

Provided is a method for preparing a peanut sprout extract having an increased nucleobase content.

The alcohol having 1 to 3 carbon atoms may be methanol, ethanol, propanol, or isopropanol, but is not limited thereto. In one embodiment, the alcohol may be ethanol or isopropanol.

The extraction solvent may contain alcohol in an amount of 10 to 100% (v/v). The alcohol content in the extraction solvent may be 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100% (v/v), or including these values as an upper limit or a lower limit. there is.

In one embodiment, the extraction solvent may contain alcohol in an amount of 20 to 40% (v/v). In another embodiment, the extraction solvent may include alcohol in an amount of 25 to 35% (v/v). In another embodiment, the extraction solvent may include alcohol in an amount of 30% (v/v). By including the extraction solvent in the above concentration range, it is possible to prepare a peanut sprout extract having an increased nucleobase content.

The extraction solvent may be a mixture further comprising water or an organic solvent.

In one embodiment, the extraction solvent may further include an acid. For example, the acids include inorganic acids such as hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, acetic acid and boric acid, or citric acid, malic acid, tartaric acid, tartaric acid, lactic acid, malic acid, tosylic acid, succinic acid, ascorbic acid, glutamic acid, alginic acid, maleic acid and phosphonic acid. It may be an organic acid such as dipic acid.

In one embodiment, the extraction solvent may be a mixture of alcohol, water, and acid having 1 to 3 carbon atoms.

In one embodiment, the extraction solvent may be a mixture containing an alcohol having 1 to 3 carbon atoms, water, and an acid in a volume ratio (%) of 1 to 4: 5 to 8: 0.005 to 0.02.

In one embodiment, the extraction solvent may be a mixture containing alcohol, water, and acid having 1 to 3 carbon atoms in a volume ratio (%) of 3: 7: 0.005 to 0.01.

For example, the extraction solvent may be a solvent having a pH of 4 to 5 prepared by mixing ethanol and water at a ratio of 30:70 and adding 0.0005 to 0.001 mL of 1N hydrochloric acid based on 1 mL of the mixture volume.

By including alcohol, water and acid in the extraction solvent in the above volume ratio range, it is possible to prepare a peanut sprout extract having an increased nucleobase content.

In one embodiment, the acid included in the extraction solvent may be hydrochloric acid.

Peanut is an annual herbaceous plant belonging to the legume family. Peanut sprout according to the present invention refers to a peanut in a sprouted state by germinating peanuts. As the raw material of the sprouted peanuts, cultivated or commercially available ones may be used without limitation.

In one embodiment, the peanut sprout may be living or dried. The dry means that the peanut sprouts are dried for 10 to 24 hours in a dryer at a temperature of 40 to 70 ℃.

The peanut sprouts may be 2 to 20 days old after germination, and in one embodiment, preferably 10 to 17 days after germination. When using peanut sprouts germinated outside the above range, the content of nucleobases obtained from the peanut sprout extract may be reduced.

In the present specification, a nucleobase is an active ingredient obtained from a peanut sprout extract and may be effective in treating, preventing, or improving osteoporosis.

In one embodiment, the nucleobase may be uracil, adenine, or a combination thereof. Uracil and adenine are representative ingredients that can be used for treatment, prevention, or improvement of osteoporosis among active ingredients obtained from peanut sprouts.

The manufacturing method according to one embodiment may prepare a peanut sprout extract having an increased total content of uracil and adenine.

In the manufacturing method according to one embodiment, the peanut sprout may be provided as a ground peanut sprout. In one embodiment, the peanut sprout pulverized product may be extracted with an extraction solvent to obtain a peanut sprout extract. The extraction solvent may include an alcohol having 1 to 3 carbon atoms, and may have a pH of 4 to 5.

In one embodiment, the peanut sprout extract extracted with the extraction solvent may further include a filtration step or a concentration step.

In one embodiment, the method for producing a peanut sprout extract with increased nucleobase content,

pulverizing peanut sprouts to obtain a pulverized product;

extracting the pulverized product with an extraction solvent to obtain an extract;

filtering the extract to obtain a filtrate; and

It may include the step of concentrating the filtrate under reduced pressure.

In one embodiment, the extraction solvent may be 30% ethanol or 30% isopropanol.

In one embodiment, the peanut sprouts may be 10 to 17 days old after germination.

The extraction temperature of the extract may be carried out at 4 ℃ to 150 ℃. In addition, the extraction time may be 12 hours to 120 hours. If the extraction temperature is lower than 4 ℃, the extraction may be difficult, and if it exceeds 150 ℃, the extract may be denatured. If the extraction time is less than 12 hours, some of the active ingredients may not be extracted, and if it exceeds 120 hours, impurities other than the active ingredients may be extracted together, which is not preferable.

The peanut sprout extract grinds the peanuts together with the peel and extracts them with an extraction solvent, removes the peanut residues, filters with filter paper, and then concentrates the filtrate using a vacuum concentrator, a vacuum rotary concentrator or a vacuum dryer, and then stores it at room temperature. can

When the extract is obtained by extracting the extract from the raw material, as the extraction method, all conventionally known extraction methods such as solvent extraction, ultrasonic extraction, filtration, and reflux extraction may be used, and preferably by using a solvent extraction method or reflux extraction method can be manufactured. The extract may be prepared by adding a fractionation solvent to an extract prepared by extracting plants, etc. with an extraction solvent or extracting them with an extraction solvent. The extraction process may be repeated several times, and thereafter, a step such as concentration or freeze-drying may be additionally performed. Specifically, the obtained extract is concentrated under reduced pressure to obtain a concentrate, and after freeze-drying the concentrate, a high concentration extract powder can be prepared using a grinder.

The method for producing the peanut sprout extract may additionally use a conventional extraction device, an ultrasonic crushing extractor or a fractionator, if necessary.

Another aspect provides a peanut sprout extract prepared by the above manufacturing method.

Another aspect provides a pharmaceutical composition for treating or preventing osteoporosis comprising the peanut sprout extract prepared by the above manufacturing method as an active ingredient.

Another aspect provides a health functional food for preventing or improving osteoporosis comprising the peanut sprout extract prepared by the above manufacturing method as an active ingredient.

The pharmaceutical composition or health functional food according to one embodiment may exhibit a therapeutic, preventive, or ameliorating effect on osteoporosis.

The pharmaceutical composition or health functional food according to one embodiment may be to promote osteoblast differentiation. The pharmaceutical composition may be to increase bone density.

In the present specification, osteoporosis includes all diseases caused by abnormality of osteoblasts such as undifferentiated, low expression, and non-expression of osteoblasts, and does not specifically mean only weakening of bone tissue that progresses with human aging.

The pharmaceutical composition or health functional food comprising the peanut sprout extract according to the present invention as an active ingredient may be applied to animals including humans.

The dosage of the pharmaceutical composition for the treatment or prevention of osteoporosis can be determined in consideration of the administration method, the age, sex, severity, condition of the patient, the absorption of the active ingredient in the body, the inactivation rate, and the drug to be used in combination, 1 Based on the daily active ingredient, 0.1 mg/kg (body weight) to 500 mg/kg (body weight), 0.1 mg/kg (body weight) to 400 mg/kg (body weight), or 1 mg/kg (body weight) to 300 mg It may be administered in /kg (body weight), and may be administered once or divided into several doses, but is not limited thereto.

The peanut sprout extract according to the present invention may be used alone in the pharmaceutical composition for treating or preventing osteoporosis, and may further include other pharmaceutically acceptable carriers. In this case, the content of the peanut sprout extract in the pharmaceutical composition for treating or preventing osteoporosis may be 0.001% to 99.9% by weight, 0.1% to 99% by weight, or 1% to 50% by weight, but is limited thereto No, the content of the compound may be appropriately adjusted to a desired content depending on the mode of use and the method of use of the composition.

Examples of the pharmaceutically acceptable carrier include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose , microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium One or more selected from the group consisting of carbonate, propylene glycol, liquid paraffin and physiological saline may be mentioned, but the present invention is not limited thereto, and any conventional pharmaceutically acceptable carrier may be used.

In addition, the pharmaceutical composition for the treatment or prevention of osteoporosis comprising the peanut sprout extract is a conventional filler, extender, binder, disintegrant, anti-aggregant, lubricant, wetting agent, pH adjuster, nutrients, vitamins, electrolytes, alginic acid and salts thereof, pect Acids and salts thereof, protective colors, glycerin, fragrances, emulsifiers or preservatives and the like may be further included.

In addition, the pharmaceutical composition for the treatment or prevention of osteoporosis of the present invention is a substance known in the art to have a known osteoporosis therapeutic effect in addition to the active ingredient, for example, human parathyroid hormone (hPTH), growth hormone, estrogen, bisphosphonate, calcitonin, etc. may additionally include a material of

When the peanut sprout extract according to the present invention is used as a pharmaceutical, the administration method includes both oral and parenteral administration. In addition, the formulation of the pharmaceutical composition for the treatment or prevention of osteoporosis may vary depending on the method of use, and it is well known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. It can be formulated using known methods.

The pharmaceutical composition according to one embodiment may be for oral or parenteral administration.

Solid preparations for oral administration include tablets, soft or hard capsules, pills, powders, suspending agents, syrups, injections, and granules, and these preparations include one or more excipients, for example, starch, calcium carbonate, sucrose or It may be prepared by mixing lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Forms for parenteral administration may be in the form of creams, lotions, ointments, warning agents, liquids, aerosols, liquid extracts, elixirs, needles, sachets, patches, or injections.

The health functional food for preventing or improving osteoporosis containing the peanut sprout extract of the present invention as an active ingredient is a food group with added value added to act and express the function of the food for a specific purpose using physical, biochemical, bioengineering methods, etc. It refers to food that has been designed and processed to sufficiently express the biological control functions of the food composition, such as regulation of biological defense rhythm, disease prevention and recovery, etc.

The health functional food for the prevention or improvement of osteoporosis may include a food supplementary additive that is pharmaceutically acceptable, and may further include an appropriate carrier commonly used in the manufacture of health functional food.

The health functional food composition for preventing or improving osteoporosis of the present invention may contain the sprouted peanut extract of the present invention in an amount of 0.001 to 99% by weight of the total food weight.

Hereinafter, examples will be given to describe the present invention in detail. However, the following examples only illustrate the present invention, and the scope of the present invention is not limited by the following examples.

Example 1. Preparation of peanut sprout extract according to the type and concentration of the extraction solvent

500 mL of an extraction solvent was added to 100 g of a peanut sprout biological sample, put together in a grinder, pulverized, and extracted by stirring at room temperature for 12 hours, followed by filtration. After repeating the extraction twice under the same conditions, it was concentrated under reduced pressure to obtain an extract.

At this time, ethanol or isopropanol was used as the extraction solvent. The concentration of each extraction solvent was adjusted to 30, 50, 60, 70, 80, 90, or 100% (v/v).

Example 2. Preparation of peanut sprout extract according to the pH of the extraction solvent

To 100 g of the peanut sprout biological sample, 500 mL of 30% ethanol as an extraction solvent was added, put together in a grinder, and then pulverized, extracted by stirring at room temperature for 12 hours, and filtered. Extraction was repeated twice under the same conditions and then concentrated under reduced pressure to obtain an extract.

At this time, the pH of the extraction solvent was adjusted to pH 3, 4, 5, 6, or 7 by adding hydrochloric acid, respectively. Mix ethanol and water 30:70, add 0.1, 0.011, 0.0006, 0.0003, and 0 mL of 1N hydrochloric acid, respectively, based on 1 mL of mixture volume, respectively, to extract having a pH of 3, 4, 5, 6, or 7, respectively A solvent was prepared.

Example 3. Preparation of peanut sprout extract according to the germination period (by growth date) of peanut sprout

To 100 g of a peanut sprout biological sample, 500 mL of 100% isopropanol as an extraction solvent was added, put together in a grinder, pulverized, and extracted by stirring at room temperature for 12 hours, followed by filtration. Extraction was repeated twice under the same conditions and then concentrated under reduced pressure to obtain an extract.

At this time, peanut sprouts germinating for 2, 4, 7, 9, 10, 12, 14, 17, or 20 days were used.

Preparation Example 1: Nucleobase Content Analysis Method of Peanut Sprout Extract

A pretreatment process was performed to measure the content of nucleobases, an active ingredient for improving osteoporosis, contained in the peanut sprout extract. Such a pretreatment process increases the content of nucleobases having basicity contained in the extract, thereby facilitating analysis.

(1) Pre-treatment process of peanut sprout extract

About 1 g of the peanut sprout extract prepared in Examples 1 to 3 was suspended in distilled water and acidified to pH 4. Then, ethyl acetate (EA) was added to perform layer separation, and a water layer was taken from the fraction. To remove acidic substances contained in the obtained water layer, the water layer was basified to pH 10, ethyl acetate (EA) was added again, and the layers were separated to obtain an EA layer containing nucleobases.

(2) Analysis of the content of nucleobases

After obtaining about 1 g of the peanut sprout extract prepared in Examples 1 to 3 through the above pretreatment process, it was dissolved in 2 mL of 50% methanol, respectively, and quantitative analysis was performed using HPLC. A calibration curve was prepared using uracil and adenine, which have relatively high contents among the nucleobases contained in the extract, as standard materials. Using this, the contents of uracil and adenine contained in the peanut sprout extract were converted and expressed.

Table 1 shows the HPLC analysis conditions of uracil and adenine.

[Table 1]

Figure 1 (a) shows the HPLC chromatogram of uracil (Uracil) and 　 adenine (Adenine) contained in the peanut sprout extract according to the present invention. 1(b) and 1(c) show calibration curves prepared using uracil and adenine as standard materials, respectively.

Test Example 1: Measurement result of yield of peanut sprout extract

The yield of the peanut sprout extract from the extracts prepared in Examples 1 to 3 was measured. 2 (a) to (d) are, respectively, the concentration % (v / v) of the ethanol extraction solvent, the concentration % (v / v) of the isopropanol extraction solvent, the pH of the ethanol-containing extraction solvent, or peanut sprouts by germination period of peanut sprouts The yield (%) (w/w) of the extract is shown.

(1) Yield of ethanol extract

As a result of preparing the extract using ethanol 30, 50, 60, 70, 80, 90, or 100% (v/v) in Example 1, 3 to 4 g of peanut sprout extract per about 100 g of raw peanut sprout obtained.

(2) Yield of isopropanol extract

As a result of preparing the extract using isopropanol 30, 50, 60, 70, 80, 90, or 100% (v/v) in Example 1, 3 to 5 g of peanut sprout extract per 100 g of raw peanut sprout obtained.

(3) Yield of extract by pH

As a result of preparing a peanut sprout extract using a 30% ethanol extraction solvent having a pH of 3, 4, 5, 6, or 7 in Example 2, 3 to 5 g of the extract per 100 g of raw peanut sprout was obtained.

(4) Yield of extract by germination period (growth date) of peanut sprout

As a result of preparing a peanut sprout extract using peanut sprouts germinated for 2, 4, 7, 9, 10, 12, 14, 17, or 20 days in Example 3, 1 to 5 g per about 100 g of raw peanut sprouts of the extract was obtained.

Test Example 2: Nucleobase content analysis result of peanut sprout extract

The content of nucleobases (content of uracil and adenine) contained in the peanut sprout extract was measured in the same manner as in Preparation Example 1 using the peanut sprout extract prepared in Examples 1 to 3.

(1) Nucleobase content in ethanol extract

In Example 1, a peanut sprout extract was prepared using ethanol 30, 50, 60, 70, 80, 90, or 100% (v/v), and the content of nucleobases contained in each extract was pre-treated as in Preparation Example 1. measured.

Figure 3 shows the total content (㎍ / g) of uracil and adenine obtained from the peanut sprout extract according to the concentration % (v / v) of the ethanol extraction solvent.

3, the 30% ethanol extract showed the highest content (93.6 μg/g) of the sum of uracil and adenine.

(2) Content of nucleobases in isopropanol extract

In Example 1, a peanut sprout extract prepared using isopropanol 30, 50, 60, 70, 80, 90, or 100% (v/v) was prepared, and the nucleobases contained in each extract were pretreated as in Preparation Example 1. The content was measured.

Figure 4 shows the total content (㎍ / g) of uracil and adenine obtained from the peanut sprout extract according to the concentration % (v / v) of the isopropanol extraction solvent.

4, the 30% isopropanol extract showed the highest content (107.1 μg/g) of the sum of uracil and adenine.

(3) Content of nucleobases in extracts by pH

In Example 2, a peanut sprout extract was prepared using a 30% ethanol extraction solvent having a pH of 3, 4, 5, 6, or 7, respectively, and the nucleobase content contained in each extract was measured by pretreatment as in Preparation Example 1. .

Figure 5 shows the total content (㎍ / g) of uracil and adenine obtained from the peanut sprout extract according to the pH of the extraction solvent.

5, the pH 4 extract showed the highest content (116.7 μg/g) of the sum of uracil and adenine.

As shown in the above results, the peanut sprout extract prepared using an extraction solvent having a pH of 4 to 5 showed a high content of nucleobases.

(4) Nucleobase content in the extract by germination period (growth date) of peanut sprouts

In Example 3, a peanut sprout extract was prepared using peanut sprouts germinated for 2, 4, 7, 9, 10, 12, 14, 17, or 20 days, and the nucleobases contained in each extract were pre-treated as in Preparation Example 1. The content was measured.

Figure 6 shows the total content (㎍ / g) of uracil and adenine obtained from the peanut sprout extract according to the germination period (growth day) of the peanut sprout.

As shown in FIG. 6 , the highest content (137.8 μg/g) of the sum of uracil and adenine was shown in the extract on the 12th day of germination.

As shown in the above results, the content of nucleobases contained in the peanut sprouts germinated for 10 to 17 days was relatively higher than in the case of germination during other periods.

Test Example 3: Content of nucleobases contained in the extract extracted with 30% ethanol or 30% isopropanol adjusted to pH 4, 5, 7 of germinated peanut sprouts on the 10th to 17th day

After extracting the peanut sprouts 10, 14, and 17 days after germination using 30% ethanol or isopropanol (pH 4, pH 5), which are the optimal extraction solvents confirmed in Test Examples 1 and 2, uracil and uracil as the content of nucleobases in the extract The total content (μg/g) of adenine is shown in FIG. 7 .

Figure 7 (a) shows the total content (㎍ / g) of uracil and adenine obtained by extracting peanut sprouts 10 days after germination with 30% ethanol or 30% isopropanol adjusted to pH 4, 5, or 7.

Figure 7 (b) shows the total content (㎍ / g) of uracil and adenine obtained by extracting peanut sprouts 14 days after germination with 30% ethanol or 30% isopropanol adjusted to pH 4, 5, or 7.

Figure 7 (c) shows the total content (㎍ / g) of uracil and adenine obtained by extracting peanut sprouts 17 days after germination with 30% ethanol or 30% isopropanol adjusted to pH 4, 5, or 7.

From the results of Figs. 7(a) to (c), higher contents of uracil and adenine were obtained from peanut sprout extract using 30% ethanol or 30% isopropanol adjusted to pH 4 to 5 than under the condition adjusted to pH 7 It was confirmed that it is possible.

This study is the result of a research conducted as a project to foster a wide-area cooperative area supported by the Ministry of Trade, Industry and Energy and the Korea Institute for Advancement of Technology (P0008626).

Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (20)

Extracting the peanut sprout with an extraction solvent to obtain a peanut sprout extract,
The extraction solvent comprises an alcohol having 1 to 3 carbon atoms, characterized in that the pH is 4 to 5,
Method for producing peanut sprout extract with increased nucleobase content. The method according to claim 1, wherein the alcohol is ethanol or isopropanol. The method according to claim 1, wherein the extraction solvent comprises alcohol in an amount of 20 to 40% (v/v). The method according to claim 1, wherein the extraction solvent comprises alcohol in an amount of 25 to 35% (v/v). The method according to claim 1, wherein the extraction solvent comprises alcohol in an amount of 30% (v/v). The method according to claim 1, wherein the extraction solvent further comprises an acid. The method according to claim 1, wherein the extraction solvent is a mixture of alcohol, water, and acid having 1 to 3 carbon atoms. The method according to claim 1, wherein the extraction solvent is a mixture containing alcohol, water and acid having 1 to 3 carbon atoms in a volume ratio (%) of 1 to 4: 5 to 8: 0.005 to 0.02. The method according to claim 1, wherein the extraction solvent is a mixture containing alcohol, water and acid having 1 to 3 carbon atoms in a volume ratio (%) of 3: 7: 0.005 to 0.01. The method according to claim 6 to 9, wherein the acid is hydrochloric acid. The method according to claim 1, wherein the peanut sprout is 10 to 17 days after germination, characterized in that the manufacturing method. The method according to claim 1, wherein the peanut sprout is a living body or a dry body, characterized in that the manufacturing method. The method of claim 1, wherein the nucleobase is uracil, adenine, or a combination thereof. The method of claim 1, wherein the total content of uracil and adenine is increased. The method according to claim 1,
pulverizing peanut sprouts to obtain a pulverized product;
extracting the pulverized product with an extraction solvent to obtain an extract;
filtering the extract to obtain a filtrate; and
Concentrating the filtrate under reduced pressure; comprising, a method for producing a peanut sprout extract with an increased content of nucleobases. The method according to claim 15, wherein the extraction solvent comprises 30% ethanol or 30% isopropanol. The method according to claim 15, wherein the peanut sprout is 10 to 17 days after germination, characterized in that the manufacturing method. A peanut sprout extract prepared by the manufacturing method of claim 1. A pharmaceutical composition for treating or preventing osteoporosis comprising the peanut sprout extract prepared by the method of claim 1 as an active ingredient. A health functional food for preventing or improving osteoporosis comprising the peanut sprout extract prepared by the manufacturing method of claim 1 as an active ingredient.

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