KR20220017060A - Composition comprising the extract of Gossypium nanking seed for preventing or treating neuro degenerative disease - Google Patents

Abstract

The present invention relates to a composition for preventing or treating degenerative brain diseases which comprises a cottonseed extract. When a cottonseed (Gossypium hirsutum L. seed) extract of the present invention is administered to a mouse whose forgetfulness is induced with Scopolamine, it is confirmed that the mouse shows the increased ability for alternation behavior in a Y-maze experiment and the inhibitory activity of acetylcholinesterase, which is an important neurotransmitter related to memory, of the mouse is exhibited. Therefore, the cottonseed (Gossypium hirsutum L. seed) extract can be useful as a food composition as well as a pharmaceutical composition for learning or memory improvement or cognitive function improvement, and treatment and prevention of degenerative diseases.

Description

Composition comprising the extract of Gossypium nanking seed for preventing or treating neurodegenerative disease, comprising a cottonseed extract

The present invention relates to a composition for preventing or treating degenerative brain disease comprising a cotton ( Gossypium nanking ) seed extract.

As living standards improve and human lifespan increases, interest in aging and various diseases related to aging is growing, and in recent years, degenerative brain diseases of the central nervous system such as Alzheimer's syndrome, Parkinson's syndrome, and Huntington's syndrome have become a major social problem. .

In particular, among degenerative brain diseases, dementia is defined as a complex of symptoms that affects both mental, cognitive and social behavioral abilities, leading to disturbances in normal daily life. It is emerging as a serious social and economical health issue for the elderly. Depending on the cause, dementia is classified into Alzheimer's disease, in which cognitive decline progresses without a clear cause, vascular dementia caused by cerebrovascular diseases such as recurrent cerebral infarction, and dementia caused by other diseases. It is known that 50-60% of all dementia patients are caused by Alzheimer's disease. In general, Alzheimer's disease patients show clinically distinct impairments in intellectual ability, emotional and behavioral changes, etc., and then show signs of serious cortical dysfunction such as loss of direction, memory impairment, and aphasia.

As the main cause of cerebrovascular dementia is infarction caused by arteriosclerosis, symptoms can be improved by treatment with thrombolytic drugs, etc. However, Alzheimer's disease rarely shows prognostic signs before the age of 50, but the prevalence tends to increase with age. It is a typical degenerative brain disease that worsens as brain cells are gradually destroyed, and the final diagnosis of this disease can only be made through pathological examination through post-mortem autopsy. Although many researchers are actively conducting research, the cause of the disease has not been fully elucidated so far.

In addition, the decline of memory and cognitive function, the primary symptom of dementia, is known to be closely related to the cholinergic nervous system, and according to reports, scopolamine, an antagonist of muscarinic receptors in postsynapse (scopolamine) temporarily blocks information transmission by inhibiting the binding of acetylcholine, a neurotransmitter released from the presynapse, with muscarinic receptors, thereby impairing learning and memory. It is commonly used in animal models of memory loss to be verified.

Currently, reversible AChE inhibitors such as donepezil, galantamine, rivastigmine, and tacrine are used as treatment for Alzheimer's disease, but these drugs only provide moderate symptom relief. There are side effects such as nausea, diarrhea, vomiting, anorexia, abdominal pain and muscle spasms related to peripheral choline excitatory effects on the gastrointestinal tract. have. Therefore, there is a need to develop a new therapeutic agent that can safely and effectively treat degenerative brain diseases such as forgetfulness and memory improvement.

Accordingly, the present inventors confirmed that the cottonseed (cottonja) extract has an effect of treating degenerative brain diseases such as forgetfulness and memory improvement, and completed the present invention.

Korean Patent Registration No. 10-1725150

It is an object of the present invention to provide a health functional food composition for preventing or improving degenerative brain disease.

Another object of the present invention is to provide a health food composition for preventing or improving degenerative brain disease.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating degenerative brain disease.

Another object of the present invention is to provide a health functional food composition for improving learning or memory or improving cognitive function.

Another object of the present invention is to provide a health food composition for improving learning or memory or improving cognitive function.

In order to achieve the above object,

The present invention provides a health functional food composition for preventing or improving degenerative brain disease, comprising a cotton ( Gossypium nanking ) seed extract.

Furthermore, the present invention provides a health food composition for preventing or improving degenerative brain disease, comprising a cotton ( Gossypium nanking ) seed extract.

In addition, the present invention provides a pharmaceutical composition for preventing or treating degenerative brain disease, comprising the cotton ( Gossypium nanking ) seed extract.

Furthermore, the present invention provides a health functional food composition for improving learning or memory or improving cognitive function, comprising a cotton ( Gossypium nanking ) seed extract.

In addition, the present invention provides a health food composition for improving learning or memory or cognitive function, comprising the cotton ( Gossypium nanking ) seed extract.

When the cotton seed (cotton seed) extract of the present invention was administered to mice induced by scopolamine for forgetfulness, it was confirmed that altered behavior increased in the Y-maze experiment, and acetyl, an important neurotransmitter related to memory As the cholinesterase inhibitory activity is confirmed, the cottonseed (cotton seed) extract may be useful as a pharmaceutical composition as well as a food composition for improving learning or memory or improving cognitive function and treating and preventing degenerative diseases.

1 shows the results of measuring the memory improvement effect of the cottonseed (cottonseed; cottonseed) extract of the present invention through a Y maze experiment, and the degree of behavioral change according to the treatment of the cottonseed extract ( Gossypium nanking ) is analyzed. (DNPZ in all figures indicates the group administered with donepezil).
Figure 2 is the result of confirming the acetylcholinesterase inhibitory activity of the cotton ( Gossypium nanking ) seed extract of the present invention.
3 is a result confirming the antioxidant activity of the cotton ( Gossypium nanking ) seed extract of the present invention.

Hereinafter, the present invention will be described in detail.

Health food or health functional food composition for preventing or improving degenerative brain disease

The present invention provides a health food or health functional food composition for preventing or improving degenerative brain disease, comprising a cotton ( Gossypium nanking ) seed extract.

The cotton ( Gossypium nanking ) seed extract according to the present invention can be used to isolate and obtain an extract from cotton ( Gossypium nanking ) seeds by using an extraction and separation method known in the art, and as defined in the present invention, ' The 'extract' can be extracted from cotton (Gossypium nanking) seeds using an appropriate solvent. In this case, as a suitable solvent that can be used to obtain the extract, any solvent acceptable in the art may be used, and extraction may be performed with water, an organic solvent, or a mixed solvent thereof. Examples of the organic solvent include, but are not limited to, various solvents such as C ₁ to C ₆ lower alcohol, hexane, acetone, ethyl acetate, chloroform, and diethyl ether, either alone or in combination. Preferably, in the case of a cotton ( Gossypium nanking ) seed extract, hexane may be used as a solvent.

When the cotton ( Gossypium nanking ) seed extract of the present invention is used as a health functional food and health food composition, the type of food is not particularly limited. Examples of health functional food to which the cotton (Gossypium nanking) seed extract of the present invention can be added may be in the form of tablets, capsules, granules, pills or liquids, and examples of health food include various drinks, meat, sausage, bread, There are candies, snacks, noodles, ice cream, dairy products, soups, ionic drinks, beverages, alcoholic beverages, gum, tea, and vitamin complexes, and includes all health functional foods and health food compositions in a conventional sense.

The health functional food and health food composition containing the cotton ( Gossypium nanking ) seed extract according to the present invention may be added to food as it is or used together with other food or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the cotton ( Gossypium nanking ) seed extract may be appropriately determined according to the purpose of its use (for prevention or improvement). In general, the amount of the composition in the health functional food and health food composition may be added in an amount of 0.1 to 90 parts by weight based on the total weight of the food. However, in the case of long-term intake for health maintenance or health control, the above amount may be less than the above range, and since there is no problem in terms of safety, the cotton ( Gossypium nanking ) seed extract can be used in an amount above the above range. can be used

The health functional food and health food composition of the present invention is not particularly limited in other ingredients except for containing the present invention cotton ( Gossypium nanking ) seed extract as an essential ingredient in the indicated ratio, and various flavoring agents or natural carbohydrates like conventional beverages and the like as additional ingredients. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 health functional food and health food composition of the present invention.

In addition to the above, the health functional food and health food composition containing the cotton ( Gossypium nanking ) seed extract of the present invention are various nutrients, vitamins, minerals (electrolytes), synthetic flavoring agents and flavoring agents such as natural flavoring agents, coloring agents and thickeners (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated drinks, etc. . In addition, the health functional food and health food composition of the present invention may contain natural fruit juice, fruit juice for the production of fruit juice drinks, and vegetable drinks.

These components may be used independently or in combination. Although the proportion of these additives is not so important, it is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the health functional food and health food composition containing the cotton ( Gossypium nanking ) seed extract of the present invention.

Pharmaceutical composition for preventing or treating degenerative brain disease

The present invention provides a pharmaceutical composition for preventing or treating degenerative brain disease, comprising a cotton ( Gossypium nanking ) seed extract.

The cotton ( Gossypium nanking ) seed extract according to the present invention can be used to isolate and obtain an extract from cotton ( Gossypium nanking ) seeds by using an extraction and separation method known in the art, and as defined in the present invention, ' The 'extract' can be extracted from cotton ( Gossypium nanking ) seeds using an appropriate solvent. In this case, as a suitable solvent that can be used to obtain the extract, any solvent acceptable in the art may be used, and extraction may be performed with water, an organic solvent, or a mixed solvent thereof. Examples of the organic solvent include, but are not limited to, various solvents such as C ₁ to C ₆ lower alcohol, hexane, acetone, ethyl acetate, chloroform, and diethyl ether, either alone or in combination. Preferably, in the case of a cotton ( Gossypium nanking ) seed extract, hexane may be used as a solvent.

As the extraction method, any one of methods such as hot water extraction, cold extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression may be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process, and may be purified using a conventional purification method. There is no limitation on the method for preparing the cotton ( Gossypium nanking ) seed extract of the present invention, and any known method may be used.

In addition, the pharmaceutical composition of the present invention may be prepared using a pharmaceutically suitable and physiologically acceptable adjuvant in addition to the active ingredient, and the adjuvant includes an excipient, a disintegrant, a sweetener, a binder, a coating agent, and a swelling agent. , lubricants, lubricants or flavoring agents may be used.

The pharmaceutical composition may be preferably formulated as a pharmaceutical composition by including one or more pharmaceutically acceptable carriers in addition to the active ingredients described above for administration.

Formulations of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops or injectables. For formulation in the form of, for example, tablets or capsules, the active ingredient may be combined with an orally, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or required, suitable binders, lubricants, disintegrants and color-developers may also be included in the mixture. Suitable binders include, but are not limited to, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tracacanth or sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like. In the composition formulated as a liquid solution, acceptable pharmaceutical carriers are sterile and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostats may be added as needed. In addition, diluents, dispersants, surfactants, binders and lubricants may be additionally added to form an injectable formulation such as an aqueous solution, suspension, emulsion, etc., pills, capsules, granules or tablets. Furthermore, by using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA by an appropriate method in the field, it can be preferably formulated according to each disease or component.

Examples of degenerative brain diseases that the cotton ( Gossypium nanking ) seed extract of the present invention can treat, prevent, and improve include Parkinson's disease, Huntington's disease, Alzheimer's disease, mild cognitive impairment ( Mild cognitive impairment, senile dementia, vascular dementia, Frontotemporal Dementia, Lewy Body Dementia, amyotrophic lateral sclerosis, spinocerebellar motor Spinocerebellar Atrophy, Tourette's Syndrome, Friedrich's Ataxia, Machado-Joseph's disease, Dystonia, Progressive supranuclear palsy Supranuclear Palsy, Pick's disease or Creutzfeldt-Jakob disease.

Health functional food or health food composition for learning or memory improvement or cognitive function improvement

The present invention provides a health functional food or health food composition for improving learning or memory or cognitive function, comprising a cotton ( Gossypium nanking ) seed extract.

The cotton ( Gossypium nanking ) seed extract according to the present invention can be used to isolate and obtain an extract from cotton ( Gossypium nanking ) seeds by using an extraction and separation method known in the art, and as defined in the present invention, ' The 'extract' can be extracted from cotton ( Gossypium nanking ) seeds using an appropriate solvent. In this case, as a suitable solvent that can be used to obtain the extract, any solvent acceptable in the art may be used, and extraction may be performed with water, an organic solvent, or a mixed solvent thereof. Examples of the organic solvent include, but are not limited to, various solvents such as C ₁ to C ₆ lower alcohol, hexane, acetone, ethyl acetate, chloroform, and diethyl ether, either alone or in combination. Preferably, in the case of a cotton ( Gossypium nanking ) seed extract, hexane may be used as a solvent.

In one embodiment of the present invention, 'cognition' refers to all processes of receiving and storing certain information in the brain, finding and using the stored information, and we think, speak, remember, and judge in our lives. and all processes to be executed are included in 'cognition'. Cognitive function refers to the ability of the brain to remember, think, judge, and execute all processes of receiving and storing information, locating and using the stored information. These cognitive functions can be broadly divided into attention, language, spatiotemporal, memory, and executive functions (or management functions).

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples only illustrate the present invention, and the content of the present invention is not limited by the following examples.

<Junbeye 1> Experimental animals

A 4-week-old ICR mouse was supplied from Daehan Biolink and used after acclimatization in a light/dark cycle for 12 hours in a breeding room maintained at temperature (22±2℃) and humidity (50±10%). Water and feed were freely ingested during the experiment period.

<Preparation Example 2> Administration of the drug

For the experimental group, motherwort was orally administered 1 hour before the start of the experiment, and 1 mg/kg of scopolamine was administered intraperitoneally 30 minutes later to induce forgetfulness in the experimental animals.

<Example 1> Preparation of cotton (Gossypium nanking) seed (cottonja) extract

Domestically produced cotton ( Gossypium nanking ) seeds (cotton jasmine) were purchased from the Korea Herbal Medicine Association (Seoul, Korea) and used. 5 L of hexane was added to 500 g of medicinal material, leached at room temperature for 24 hours, and the extract was filtered through filter paper (Advantec No. The extract was concentrated under reduced pressure at 30°C to remove the solvent and stored at -20°C for use in the experiment (yield: 12.61%).

<Experimental Example 1> Y-maze test (Y-maze test)

Cotton ( Gossypium nanking ) of the present invention In order to evaluate the short-term memory and behavioral ability in the forgetfulness-induced mouse of the seed (cottonja) extract, a Y-maze experiment was performed. The measuring instrument consists of three branches, each branch is 42 cm long, 3 cm wide, and 12 cm high, and the angle of the three branches is 120°. This device is made of black polyvinyl plastic, and three branches were assigned to A, B, and C, respectively, and then the experiment was conducted. One point (actual alternation) was given by counting the number of times the animals entered each branch from the tail to the tail of the experimental animal and the cases in which they entered each branch in turn for 8 minutes. Alteration behavior is defined as not overlapping all three, and is calculated by the following equation.

Change action (%) = actual alternation / maximum alternation × 100 (maximum change: total number of entries - 2)

As a result, as shown in Figure 1, the CON group administered with scopolamine was 60.7±1.2%, compared to 73.8±1.75% of the normal NOR group, the spontaneous alterations (%) were significantly reduced, Example In the group administered with 50, 100, or 200 mg/kg of cotton ( Gossypium nanking ) seed (cotton seed) extract of 1, the altered behavior was significantly increased at 62.7±3.3%, 73.0±2.6%, and 71.0±1.4% at high concentrations. could see In the donepezil-administered group, which was used as a positive control, it was 73.5±2.3%, which was similar to that of the normal group. The increase in altered behavior means that learning and memory were restored, while the total number of entry into each branch did not change, suggesting that this result was not caused by the change in the activity of the mouse.

Therefore, the restoration of short-term memory and behavioral ability of cotton ( Gossypium nanking ) seed (cottonja) extract was confirmed through the experimental results.

<Experimental Example 2> Measurement of acetylcholinesterase inhibitory activity

Acetylcholinesterase is an enzyme that decomposes acetylcholine, an important neurotransmitter related to memory. In order to confirm this, Ellman's method was tested by colorimetric analysis using acetylthiocholine iodide (ASCh) as a substrate. After cervical dislocation of the experimental animal not used for the experiment, the whole brain was removed, homogenized using cold sodium phosphate buffer (100 mM, pH 8.0), and the AChE activity was measured using the supernatant obtained after centrifugation at 12,500 rpm for 20 minutes. It was used as an enzyme for measurement. The sample and donepezil (DNPZ) used as a positive control were diluted stepwise and used. Add 134 µL of sodium phosphate buffer, 1 µL of 75 mM ASCh, 5 µL of 10 mM Ellman's solution (5,5'-dithiobis-2-nitrobenzoic acid), 50 µl of sample or DNPZ, and react at 25°C for 10 minutes, followed by 10 µL of enzyme source was added and reacted at 25 °C for 5 minutes, and absorbance was measured at 405 nm.

As a result, as shown in FIG. 2, the IC50 value for inhibiting acetylcholinesterase of the cotton ( Gossypium nanking ) seed (cottonja) extract of Example 1 was 0.9127 μg/mL and donepezil used as a positive control. ) was found to be 0.1459 μg/mL, confirming that the cotton ( Gossypium nanking ) seed (cottonja) extract of Example 1 of the present invention inhibited the activity of acetylcholinesterase.

<Experimental Example 3> Measurement of antioxidant activity

The brain is fatal to oxidative stress compared to other organs, but dementia-related amyloid beta ( Aβ) damages mitochondria and generates many reactive oxygen species. The antioxidant activity of the cotton ( Gossypium nanking ) seed (cottonza) extract of the present invention was measured.

<3-1> DPPH radical scavenging activity measurement

1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was measured to measure the antioxidant effect of the cotton ( Gossypium nanking ) seed (cottonja) extract of the present invention. DPPH is a chemically stabilized free radical, dark purple DPPH, which is reduced by aromatic amines and loses color to measure the activity of antioxidants. Dissolve each sample in distilled water and add 0.2 mM DPPH solution and 100 μL After mixing in equal amounts and reacting in a dark room for 30 minutes, absorbance was measured at 517 nm. The positive control group was comparatively analyzed using ascorbic acid, and the free radical scavenging ability was calculated using the following equation.

DPPH radical scavenging activity (%) = 100-[(absorbance of sample addition / absorbance of no addition) × 100]

<3-2> ABTS radical scavenging activity measurement

In order to measure the antioxidant effect of the cotton ( Gossypium nanking ) extract of the present invention, the radical scavenging activity of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) was measured. By reacting with ABTS and potassium persulfate to generate dark blue-green stabilized ABTS free radicals, which are decolorized by antioxidants, the activity of antioxidants is measured. 7 mM ABTS and 2.4 mM potassium persulfate are mixed 1:1 ( v:v) and reacted in a cool and dark place for 12 to 16 hours to form ABTS+, and then an ABTS substrate solution diluted with ethanol to an absorbance of 0.7±0.03 at 734 nm was used. After dissolving each sample in distilled water, 100 μL of ABTS+ solution was mixed in the same amount, reacted in the dark for 7 minutes, and absorbance was measured at 734 nm. The positive control group was comparatively analyzed using ascorbic acid, and the free radial scavenging ability was calculated using the following equation.

ABTS radical scavenging activity (%) = 100-[(absorbance of sample addition / absorbance of no-addition group) × 100]

As a result of measuring the DPPH and ABTS radical scavenging activity of the Gossypium nanking seed (cottonja) extract of the present invention, as shown in FIG. 3 , it was confirmed that the antioxidant activity was increased in a concentration-dependent manner.

Statistical processing

All experimental results were statistically processed using one way analysis of variance, and if significance was recognized, significance was tested at p<0.05 level or less using the Student-Newman-Keuls test between groups. was carried out.

So far, the present invention has been looked at with respect to preferred embodiments thereof. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is particularly indicated in the claims rather than the foregoing description, and all differences within an equivalent scope should be construed as being included in the present invention.

Preparation example of drug

The cotton seed (cotton seed) extract according to the present invention can be formulated in various forms depending on the purpose. The following exemplifies several formulation methods containing the extract of cottonseed (cotton seed) according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Pharmaceutical Preparation Example 1> Preparation of powder

Cottonseed (Cotton Seed) Extract 2 g

lactose 1 g

After mixing the above ingredients, the powder was prepared by filling in an airtight cloth.

<Pharmaceutical Preparation Example 2> Preparation of tablets

Cottonseed (Cotton Seed) Extract 100 mg

corn starch 100 mg

lactose 100 mg

magnesium stearate 2 mg

After mixing the above ingredients, tablets were prepared by tableting according to a conventional manufacturing method of tablets.

<Pharmaceutical Preparation Example 3> Preparation of capsules

Cottonseed (Cotton Seed) Extract 100 mg

corn starch 100 mg

lactose 100 mg

magnesium stearate 2 mg

After mixing the above ingredients, the capsules were prepared by filling in gelatin capsules according to a conventional manufacturing method of capsules.

<Pharmaceutical Preparation Example 4> Preparation of injection

Cottonseed (Cotton Seed) Extract 10 μg/ml

dilute hydrochloric acid BP until pH 3.5

Sodium Chloride BP for Injection up to 1 ml

Dissolve the cottonseed (cotton seed) extract according to the present invention in an appropriate volume of sodium chloride BP for injection, and adjust the pH of the resulting solution to pH 3.5 with dilute hydrochloric acid BP, and adjust the volume with sodium chloride BP for injection and sufficiently mixed. The solution was filled in a 5 ml Type I ampoule made of clear glass, sealed under an upper grid of air by dissolving the glass, and sterilized by autoclaving at 120° C. for 15 minutes or more to prepare an injection solution.

<Pharmaceutical Preparation Example 5> Preparation of nasal absorbent (Nasal spray)

Cottonseed (Cotton Seed) Extract 1.0 g

sodium acetate 0.3 g

methylparaben 0.1 g

Propylparaben 0.02 g

sodium chloride appropriate amount

HCl or NaOH pH adjustment appropriate amount

Purified water appropriate amount

According to a conventional method for preparing nasal absorbents, 3 mg of cottonseed (cotton seed) extract per 1 mL of saline (0.9% NaCl, w/v, solvent is purified water) is prepared, filled in an opaque spray container, and sterilized to prepare a nasal absorbent.

<Pharmaceutical Preparation Example 6> Preparation of liquid preparation

Cottonseed (Cotton Seed) Extract 100 mg

Lee Seonghwadang 10 g

mannitol 5 g

Purified water appropriate amount

According to a conventional liquid preparation method, each component is added to purified water to dissolve, lemon flavor is added, the above components are mixed, and purified water is added to adjust the total volume to 100 mL, then filled in a brown bottle and sterilized to prepare a liquid preparation did

Manufacturing example of health food

The cottonseed (cottonja) extract according to the present invention can be prepared into various types of health food depending on the purpose. The following exemplifies the manufacturing method of several health foods containing the extract of cottonseed (cotton seed) according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Health food production example 1> Production of dairy products

0.01-1 parts by weight of the cottonseed extract of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<Health food production example 2> Preparation of wire

Brown rice, barley, glutinous rice, and barley radish were pregelatinized by a known method and dried, and then roasted and prepared as a powder having a particle size of 60 mesh with a grinder. Black soybeans, black sesame, and perilla were also steamed and dried by a known method, and then roasted and prepared into powder having a particle size of 60 mesh with a grinder. The cotton seed extract of the present invention was concentrated under reduced pressure in a vacuum concentrator to obtain a dry powder. It was prepared by mixing the dry powder of the above-prepared grains, seeds, and cottonseed (cottonja) extract in the following proportions.

Grains (34 parts by weight of brown rice, 19 parts by weight of barley, 20 parts by weight of barley),

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame),

Cottonseed (cotton seed) extract (2 parts by weight),

Reishi (1.5 parts by weight), and

Rehmannia (1.5 parts by weight).

Manufacturing example of health functional food

The cotton seed (cotton seed) extract according to the present invention can be prepared into various types of health functional foods according to the purpose. The following exemplifies the manufacturing method of several health functional foods containing the extract of cottonseed (cotton seed) according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Health functional food manufacturing example 1> Manufacturing of health functional food

Cottonseed (Cotton Seed) Extract 100 mg

vitamin mixture appropriate amount

vitamin A acetate 70 μg

vitamin E 1.0 mg

vitamin B1 0.13 mg

vitamin B2 0.15 mg

vitamin B6 0.5 mg

vitamin B12 0.2 μg

vitamin C 10 mg

biotin 10 μg

Nicotinamide 1.7 mg

folic acid 50 μg

Calcium Pantothenate 0.5 mg

mineral mixture appropriate amount

ferrous sulfate 1.75 mg

zinc oxide 0.82 mg

magnesium carbonate 25.3 mg

monobasic potassium phosphate 15 mg

dicalcium phosphate 55 mg

potassium citrate 90 mg

calcium carbonate 100 mg

magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for health functional food, but the composition is mixed in a preferred embodiment. Then, the granules can be prepared and used in the production of a health functional food composition according to a conventional method.

<Health functional food production example 2> Preparation of health functional beverage

Cottonseed (Cotton Seed) Extract 100 mg

citric acid 100 mg

oligosaccharide 100 mg

Plum Concentrate 2 mg

Taurine 100 mg

Purified water is added to 500 mL

After mixing the above ingredients according to a conventional health drink manufacturing method, stirring and heating at 85° C. for about 1 hour, the resulting solution is filtered and obtained in one sterilized container, sealed and sterilized, and then refrigerated. used in the manufacture of health beverage compositions. Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demand class, demanding country, and use purpose.

Claims (16)

Cotton ( Gossypium nanking ) Health functional food composition for the prevention or improvement of degenerative brain disease comprising a seed extract. The method of claim 1,
The degenerative brain disease is Parkinson's disease, Huntington's disease, Alzheimer's disease, mild cognitive impairment, senile dementia, vascular dementia, frontotemporal lobe Frontotemporal Dementia, Lewy Body Dementia, amyotrophic lateral sclerosis, Spinocerebellar Atrophy, Tourette's Syndrome, Friedrich's ataxia s Ataxia), Machado-Joseph's disease, Dystonia, Progressive Supranuclear Palsy, Pick's disease and Creutzfeldt-Jakob disease Health functional food composition, characterized in that any one disease selected from the group consisting of. Cotton ( Gossypium nanking ) Health functional food composition for improving learning or memory or cognitive function, comprising a seed extract. 4. The method of claim 1 or 3,
The extract is a health functional food composition, characterized in that extracted with water, an organic solvent, or a mixed solvent thereof. 5. The method of claim 4,
The organic solvent is C ₁ to C ₆ Health functional food composition, characterized in that at least one solvent selected from the group consisting of lower alcohol, hexane, acetone, ethyl acetate, chlorophor and diethyl ether. 4. The method of claim 1 or 3,
The health functional food is a health functional food composition, characterized in that the food in the form of tablets, capsules, pills or liquids. Cotton ( Gossypium nanking ) Health food composition for preventing or improving degenerative brain disease comprising a seed extract. 8. The method of claim 7,
The degenerative brain disease is Alzheimer's disease, dementia, Parkinson's disease, amyotrophic lateral sclerosis, Huntington's disease, Pick's disease, Creutzfeldt-Jakob disease, selected from the group consisting of forgetfulness and memory impairment A health food composition, characterized in that it is any one disease. Cotton ( Gossypium nanking ) A health food composition for improving learning or memory or cognitive function comprising a seed extract. 10. The method according to claim 7 or 9,
The extract is a health food composition, characterized in that extracted with water, an organic solvent, or a mixed solvent thereof. 11. The method of claim 10,
The organic solvent is C ₁ to C ₆ Health food composition, characterized in that at least one solvent selected from the group consisting of lower alcohol, hexane, acetone, ethyl acetate, chlorophor and diethyl ether. 10. The method according to claim 7 or 9,
The health food is selected from various drinks, meat, sausage, bread, candy, snacks, noodles, ice cream, dairy products, soup, ionic beverage, beverage, alcoholic beverage, gum, tea and vitamin complex. health food composition. Cotton ( Gossypium nanking ) A pharmaceutical composition for the prevention or treatment of degenerative brain disease comprising a seed extract. 14. The method of claim 13,
The extract is a pharmaceutical composition for the prevention or treatment of degenerative brain disease, characterized in that extracted with water, an organic solvent, or a mixed solvent thereof. 15. The method of claim 14,
The organic solvent is C ₁ to C ₆ A pharmaceutical composition for the prevention or treatment of degenerative brain disease, characterized in that at least one solvent selected from the group consisting of lower alcohol, hexane, acetone, ethyl acetate, chloropo and diethyl ether . 14. The method of claim 13,
The degenerative brain disease is Parkinson's disease, Huntington's disease, Alzheimer's disease, mild cognitive impairment, senile dementia, vascular dementia, frontotemporal lobe Frontotemporal Dementia, Lewy Body Dementia, amyotrophic lateral sclerosis, Spinocerebellar Atrophy, Tourette's Syndrome, Friedrich's s Ataxia), Machado-Joseph's disease, Dystonia, Progressive Supranuclear Palsy, Pick's disease and Creutzfeldt-Jakob disease Pharmaceutical composition, characterized in that any one disease selected from the group consisting of.

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