KR20210146469A - Tri-layered liposome composition comprising cationic polymer conjugate and liposome, and cosmetic composition comprising same - Google Patents

Abstract

The present invention relates to a tri-layer liposome composition comprising liposomes coated with a cationic polymer conjugate through electrostatic bonds. In addition, the present invention relates to a cosmetic composition comprising the tri-layer liposome composition. The tuplesome of the present invention efficiently delivers an active ingredient into the skin in a very stable form, so that even with a small amount of the active ingredient, it can show a very excellent effect for a long period of time.

Description

Triple-layer liposome composition comprising a cationic polymer conjugate and liposome, and a cosmetic composition comprising the same

The present invention relates to a triple-layered liposome composition in which a liposome is coated with a cationic polymer conjugate through electrostatic bonding, and more particularly, i) an active ingredient, hydrogenated lecithin, PEG-5 rape seed sterol, cholesterol, three liposomes comprising test-3, cetes-5, plant sterols and water; And ii) as a coating layer, it relates to a triple-layered liposome composition comprising a pullulan acetate-polyethyleneimine conjugate.

In addition, the present invention relates to a cosmetic composition comprising the triple-layered liposome composition.

It is not easy to penetrate the active (effective) substances of cosmetics into the skin. Human skin plays a role in preventing intrusion from the outside and preventing evaporation of moisture inside the skin. Due to this, the delivery of the active substance to the dermis is difficult due to the stratum corneum and epidermis acting as a barrier, and the efficiency is lowered, so that it is difficult to deliver the active substance. To this end, in order to stabilize the active ingredient while enhancing the transdermal permeation efficiency, efforts and research to utilize the transdermal delivery system (TDS) in functional cosmetics are active. Due to the development of functional materials, various functionalization methods that can impart higher stability to these materials have been extensively studied.

This transdermal delivery system is mainly used as a cosmetic material because of the advantage that lipids are a component of biological membranes, and are mainly used as materials such as surfactants, lipids, and polymers to make and apply vesicles containing effective substances. have. Lipids are common compounds in nature, and nanoemulsions and liposomes using lipid vesicles (liposomes and pro liposomes) and nonionic surfactant vesicles (niosomes and pro niosomes) are used as penetration methods using them.

Liposomes are composed of a limited lipid bilayer by a lipid bilayer, and this lipid bilayer may have reduced stability due to external environments (pH, temperature, osmosis, enzymes, etc.). Various efforts have been made to promote liposome stabilization or skin permeation, and studies related to cationic polymers were first started in 1989. As an example of using such a cationic polymer, in Korean Patent Application Laid-Open No. 10-2018-0012119, a PEI (polyethyleneimine)-CA (cinnamic acid) salt bridge conjugate showing heat sensitivity is immobilized on the surface of the liposome to respond to temperature. Disclosed is an invention that releases contents in response to temperature changes by making liposomes.

Republic of Korea Patent Publication No. 10-2012-0001214 Republic of Korea Patent Publication No. 10-2018-0011219

An object of the present invention is to provide a novel liposome composition for transdermal delivery that can efficiently deliver an active ingredient into the skin in a stable state.

Another technical object of the present invention is to provide a cosmetic composition comprising the liposome composition for transdermal delivery.

In order to solve the above problems, the present invention provides: i) a liposome comprising an active ingredient, hydrogenated lecithin, PEG-5 leucid sterol, cholesterol, ceteth-3, cetes-5, plant sterol and water; and ii) a pullulan acetate-polyethyleneimine conjugate as a coating layer.

In addition, the present invention provides a cosmetic composition comprising the triple-layered liposome composition.

Hereinafter, the present invention will be described in detail.

According to one aspect of the present invention, i) active ingredient, hydrogenated lecithin, PEG-5 rapeseed sterol, cholesterol, ceteth-3, ceteth-5 ( ceteth-5), liposomes comprising plant sterols and water; And ii) a pullulan acetate-polyethyleneimine conjugate as a coating layer is provided as a triple-layered liposome composition.

A liposome is a spherical vesicle composed of a lipid bilayer, and the triple-layered liposome composition according to the present invention contains hydrogenated lecithin, PEG-5 leipseed sterol, cholesterol, ceteth-3, ceteth-5 and vegetable It has excellent stability including sterol as well as a pullulan acetate-polyethyleneimine conjugate bonded to the surface of the liposome by electrostatic attraction as a coating layer to improve stability and skin permeability. named “Terplosome” in

The tuplesome of the present invention preferably contains 0.0005 to 50% by weight, more preferably 0.0008 to 45% by weight, and still more preferably 0.001 to 40% by weight of the active ingredient. In the present invention, when the active ingredient is included in an amount of less than 0.0005% by weight, the resulting efficacy may not be properly exhibited, and when included in an amount exceeding 50% by weight, there may be a problem in the formation of liposomes.

The tuplesome of the present invention preferably contains 0.1 to 20% by weight, more preferably 0.5 to 18% by weight, and still more preferably 1 to 15% by weight of hydrogenated lecithin. Lecithin refers to a mixture of various phospholipids, and the composition of the phospholipids may vary depending on their origin. Hydrogenated lecithin can be obtained by adding hydrogen to lecithin. Hydrogenated lecithin in the present invention forms a lipid bilayer of liposomes. In the present invention, when hydrogenated lecithin is included in less than 0.1 wt%, there may be a problem in the formation of liposomes, and when included in excess of 20 wt%, the amount of active ingredient encapsulated in the liposome is reduced due to an excess of membrane components. There may be a problem.

The tuplesome of the present invention contains PEG-5 rapeseed sterol in an amount of preferably 0.1 to 15% by weight, more preferably 0.3 to 12% by weight, more preferably 0.5 to 10% by weight. include In the present invention, PEG-5 rape seed sterol helps to form liposomes and provides stability to the double membrane of liposomes. In the present invention, when PEG-5 rapeseed sterol is included in an amount of less than 0.1% by weight, the effect of PEG-5 rapeseed sterol may be weak when forming liposomes, and when it contains more than 15% by weight, liposomes may become unstable. have.

The tuplesome of the present invention contains cholesterol in an amount of preferably 0.01 to 10% by weight, more preferably 0.05 to 8% by weight, and still more preferably 0.1 to 5% by weight. In the present invention, cholesterol imparts stability to the double membrane of the liposome. In the present invention, when cholesterol is included in less than 0.01% by weight, the effect by cholesterol may be weak, and when included in excess of 10% by weight, there may be a problem in the formation of liposomes.

The tuplersome of the present invention is ceteth-3 (ceteth-3, IUPAC name: 2-[2-(2-hexadecyloxy)ethoxy]ethanol) and ceteth-5 (ceteth-5, IUPAC name: 3,6, 9,12,15-pentaoxahentriacontan-1-ol) each preferably in an amount of 0.01 to 5% by weight, more preferably 0.05 to 4% by weight, still more preferably 0.1 to 3% by weight. In the present invention, Ceteth-3 and Ceteth-5 impart stability to the double membrane of the liposome. In the present invention, when Ceteth-3 and Ceteth-5 are included in an amount of less than 0.01% by weight or more than 5% by weight, respectively, there may be a problem in the formation of liposomes.

The tuplesome of the present invention preferably contains 0.1 to 15% by weight of plant sterols, more preferably 0.5 to 12% by weight, and still more preferably 1 to 10% by weight. In the present invention, plant sterols impart stability to the double membrane of liposomes. In one embodiment according to the present invention, the plant sterols are, for example, rapeseed sterol, avocadosterol, PEG-15 phytosterol, acipamsterol, phytosteryl macadamate, soysterol, dolconsterol, canola. It may be at least one selected from the group consisting of sterols, rice bran sterols and pomegranate sterols, but is not limited thereto. In the present invention, when the plant sterol is included in an amount of less than 0.1% by weight, the effect of imparting stability by the plant sterol may be weak, and when it is included in an amount exceeding 15% by weight, there may be a problem in the formation of liposomes.

The tuplesome of the present invention preferably contains 40 to 95% by weight of water, more preferably 43 to 93% by weight, and still more preferably 45 to 90% by weight of water.

There is no specific limitation depending on the active ingredient in the tuplesome of the present invention. In the present invention, the active ingredient is, for example, a moisturizer, a whitening agent, an anti-wrinkle agent, a sunscreen agent, a hair restorer, a vitamin or derivative thereof, an amino acid or peptide, an anti-inflammatory agent, an acne treatment agent, a bactericide, a female hormone agent, a keratin exfoliating agent, and a group consisting of natural products. It may be one or more selected from, but is not limited thereto.

Moisturizers include, but are not limited to, creatine, polyglutamic acid, sodium lactate, hydroproline, 2-pyrrolidone-5-carbonate sodium, hyaluronic acid, sodium hyaluronate, ceramide, phytosterol, cholesterol, sitosterol, proteoglycan, etc. it is not Examples of the whitening agent include, but are not limited to, arbutin and arbutin derivatives, kojic acid, bisabolol, niacinamide, vitamin C and vitamin C derivatives, placenta, allantoin, and the like. The anti-wrinkle agent includes, for example, retinol, a retinol derivative, adenosine, a licorice extract, a red ginseng extract, a ginseng extract, and the like, but is not limited thereto. Examples of the sunscreen include, but are not limited to, benzophenone derivatives, para-aminobenzoic acid derivatives, methoxycinnamic acid derivatives, and salicylic acid derivatives. It will be said that there is no particular limitation as a hair restorer, but a blood circulation promoter and/or a local stimulant are preferable, and the blood circulation promoter includes, for example, Monnaceae extract, ceparatin, vitamin E and its derivatives, gamma oryzanol, etc., but are limited thereto. No, local irritants include, but are not limited to, red pepper tincture, ginger tincture, cantalis tincture, nicotinic acid benzyl ester, and the like. Vitamins or derivatives thereof include, for example, vitamin A (retinol) and its derivatives, vitamins B1, B2, B6, vitamin E and its derivatives, vitamin D, vitamin H, vitamin K, pantothenic acid and its derivatives, biotin, panthenol, Coenzyme Q ₁₀ , idebenone, and the like, but is not limited thereto. Amino acids or peptides include, for example, cystine, cysteine, methionine, serine, lysine, tryptophan, amino acid extract, epidermal growth factor (EGF), insulin-like growth factor (IGF), fibroblast growth factor (FGF), Cooper tree peptide-1, dipeptide-15, tripeptide-29, tripeptide-1, acetyl hexapeptide-8, nicotinoyl tripeptide-35, hexapeptide-12, hexapeptide-9. Palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, palmitoyl tripeptide-29, palmitoyl tripeptide-1, palmitoyl pentapeptide-3, nonapeptide-7, tripeptide-10 citrulline, sh - Polypeptide-15, palmitoyl tripeptide-5, diaminopropioyl tripeptide-33, r-spider polypeptide-1, etc., but are not limited thereto. Examples of the anti-inflammatory agent include, but are not limited to, beta-glycylic acid, glitylitanic acid derivatives, aminocaproic acid, hydrocortisone, beta-glucan, madekaside, licorice, and the like. Acne treatment agents include, but are not limited to, for example, estradiol, estrogen, ethynyl estrariol, tricrosan, azelic acid, and the like. The disinfectant includes, for example, benzalkonium chloride, benzethonium chloride, halocalban, and the like, but is not limited thereto. It will be said that there is no particular limitation as a female hormone agent, but estrogen is suitable, and estradiol, ethinyl estradiol, and isoflavones, which are phytoestrogens, etc. are preferable as estrogen. Examples of the exfoliating agent include, but are not limited to, sulfur, salicylic acid, AHA, BHA, resorcin, and the like. Extracts from natural products or ingredients obtained from them, for example, fern, chrysanthemum, chrysanthemum, rhubarb, licorice, aloe, chamomile, rosehip, ginkgo leaf, horse chestnut, ginseng, loofah, cucumber, seaweed, seaweed, kelp, hemp, snail , young woman, extracts such as green tea, curcumin, hinokitiol, beta-carotene, etc., but is not limited thereto.

In addition, as an active ingredient, a cosmetic ingredient such as oil, wax, butter, paraffin, a higher fatty acid such as stearic acid, an ester such as cetylethylhexanoate, or silicone may be used. The oil includes, for example, olive oil, camellia oil, avocado oil, macadamia oil, castor oil, sunflower oil, jojoba oil, almond oil, apricot seed oil, green tea oil, meadowfoam seed oil or argan oil. However, the present invention is not limited thereto. The wax includes, for example, carnauba wax, candelilla wax, jojoba oil, beeswax, lanolin, soy wax, rice wax, silicone wax, and the like, but is not limited thereto. The butter includes, for example, shea butter, mango butter, green tea butter, or soy butter, but is not limited thereto. The hydrocarbon includes, for example, liquid paraffin, paraffin, petrolatum, ceresin, microcrystalline wax or squalane, but is not limited thereto. Examples of the higher fatty acids include, but are not limited to, lauric acid, myristic acid, palmitic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, or linolenic acid. Examples of the higher alcohol include, but are not limited to, lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, oleyl alcohol, or behenyl alcohol. Esters include, but are not limited to, for example, 2-octyldodecyl myristate, diisostearyl maleate, or cetyl ethylhexanoate. The silicone includes, for example, dimethicones, cyclomethicones, silicone polymers, or silicone oil, but is not limited thereto.

Others include yeast extract, collagen, elastin, aluminum sucrose octasulfate, DHA, EPA, and fragrance ingredients.

The tuplesome of the present invention includes a pullulan acetate-polyethyleneimine conjugate as a coating layer. In the tuplesome of the present invention, a triple-layered liposome is formed by coating the surface of an anionic liposome with a cationic pullulan acetate-polyethyleneimine conjugate through electrostatic bonding (FIG. 1). The anionicity of the liposome can be confirmed by measuring the zeta potential, and in the liposome of the present invention, it is considered to be anionic by the (-) charge of _{the phosphate (PO 4 ) group of lecithin.} Pullulan is known as a polysaccharide with high biocompatibility and biodegradability without toxicity, and has excellent adhesion properties, so that it can adhere well to the skin. In one embodiment according to the present invention, 0.01 to 5 parts by weight of pullulan acetate-polyethyleneimine conjugate is included with respect to 100 parts by weight of the liposome.

The pullulan acetate-polyethyleneimine conjugate has the structure of Formula 1 below, synthesized by acetylating pullulan to synthesize a hydrophobic pullulan derivative, polymerizing the hydrophobic pullulan derivative and a crosslinking agent, and then polymerizing the polymer with polyethyleneimine can be synthesized by:

[Formula 1]

The content related to the synthesis of pullulan acetate-polyethyleneimine conjugate is described in detail in Korean Patent Application Laid-Open No. 10-2012-0001214 (published date: January 04, 2012), and the document is herein incorporated in its entirety. It is incorporated by reference.

According to another aspect of the present invention, there is provided a cosmetic composition comprising the tuplesome of the present invention. In the present invention, the cosmetic composition may be formulated as, for example, skin, lotion, body lotion, cream, essence, etc., but is not limited thereto.

The cosmetic composition of the present invention preferably contains 1 to 60% by weight of the tuplesome according to the present invention, more preferably 2 to 50% by weight. In the present invention, if the cosmetic composition contains less than 1% by weight of tuplersome, the effect by the active ingredient may be insignificant, and even if it contains more than 60% by weight, the effect of the active ingredient increases in proportion to the addition thereof. It is not desirable from an economic point of view because it is difficult to expect any more.

The tuplesome of the present invention efficiently delivers the active ingredient into the skin in a very stable form, so that even a small amount of the active ingredient can show a very excellent effect for a long period of time.

1 is a schematic diagram showing the structures of a tupleosome and a liposome (left: tuplesome, right: liposome).
2 is a result of measuring the particle diameter size of the tuplesome using Photal, ELS-Z.
3 is an enlarged photograph of tuplersomes using a frozen electron microscope.
4 is a result of measuring the zeta potential using Photal and ELS-Z to measure the stability of the tuplesome.
5 is a result of measuring the stability of the tuplersome with Turbiscan.
6 is a result showing a comparison of the skin permeability of a general liposome and tuplesome.

Hereinafter, the present invention will be described in more detail through examples. However, the following examples are merely illustrative to aid the understanding of the present invention, and the scope of the present invention is not limited thereto.

Preparation Example: Preparation of pullulan acetate-polyethyleneimine (PEI) conjugate

Pullulan acetate was synthesized by reacting pullulan with pyridine and acetic anhydride in the same manner as in Example 1 of Korean Patent Application Laid-Open No. 10-2012-0001214, and then the hydroxy (-OH) group of pullulan acetate was activated. Then, it was reacted with polyethyleneimine to synthesize a pullulan acetate-polyethyleneimine conjugate.

Example 1: Preparation of tuplesome containing plant natural products

After introducing the components of the liposome with the composition of Table 1 below to a container and dissolving it at a temperature of 80 ° C., after mixing for 5 minutes using a homomixer, it is passed three times at 1,000 bar in a high-pressure homogenizer Cooling and defoaming were performed to form liposomes, and a pullulan acetate-polyethyleneimine (PEI) conjugate was added and stirred to prepare tuplarsomes.

Example 2: Preparation of tuplersomes containing marine natural products

It was prepared in the same manner as in Example 1 with the composition shown in Table 2 below.

Example 3: Preparation of tuplesomes containing oil

It was prepared in the same manner as in Example 1 with the composition shown in Table 3 below.

Example 4: Preparation of Tuplesomes Containing Wax

It was prepared in the same manner as in Example 1 with the composition shown in Table 4 below.

Example 5: Preparation of tuplersomes containing butter

It was prepared in the same manner as in Example 1 with the composition shown in Table 5 below.

Example 6: Preparation of tuplersomes containing paraffin

It was prepared in the same manner as in Example 1 with the composition shown in Table 6 below.

Example 7: Preparation of tuplersomes containing higher fatty acids

It was prepared in the same manner as in Example 1 with the composition shown in Table 7 below.

Example 8: Preparation of tuplesomes containing esters

It was prepared in the same manner as in Example 1 with the composition shown in Table 8 below.

Example 9: Preparation of tuplersomes containing silicone

It was prepared in the same manner as in Example 1 with the composition shown in Table 9 below.

Example 10: Preparation of Tuplesomes Containing Moisturizer

It was prepared in the same manner as in Example 1 with the composition shown in Table 10 below.

Example 11: Preparation of tuplersomes containing a whitening agent

It was prepared in the same manner as in Example 1 with the composition shown in Table 11 below.

Example 12: Preparation of tuplersomes containing sunscreen

It was prepared in the same manner as in Example 1 with the composition shown in Table 12 below.

Example 13: Preparation of tuplersomes containing vitamins

It was prepared in the same manner as in Example 1 with the composition shown in Table 13 below.

Example 14: Preparation of tuplesomes containing amino acids and peptides

It was prepared in the same manner as in Example 1 with the composition shown in Table 14 below.

Example 15: Preparation of tuplesomes containing peptides

It was prepared in the same manner as in Example 1 with the composition shown in Table 15 below.

Example 16: Preparation of tuplesomes containing anti-inflammatory agents

It was prepared in the same manner as in Example 1 with the composition shown in Table 16 below.

Example 17: Preparation of tuplersomes containing an acne treatment agent

It was prepared in the same manner as in Example 1 with the composition shown in Table 17 below.

Example 18: Preparation of Tuplesomes Containing Fungicides

It was prepared in the same manner as in Example 1 with the composition shown in Table 18 below.

Experimental Example 1: Measurement of particle distribution

The particle distribution of the tuplesome prepared in Example 1 was measured using Photal, ELS-Z, and is shown in FIG. 2 . As a result of the measurement, it was found that the average particle size of the composite was 858.6 nm.

Experimental Example 2: Freeze electron microscopy

The tuplersomes prepared in Example 1 were photographed. Since the particle size is too fine to measure with a general optical microscope, the image was taken using a frozen electron microscope (JEM 1010, JEOL, Japan) (FIG. 3). It was found that the tuplarsome was well formed.

Experimental Example 3: Measurement of stability of tuplesomes

To measure the stability of the tuplesome prepared in Example 1, the zeta potential was measured using Photal and ELS-Z, and the results are shown in FIG. 4 . As a result of the measurement, it was found that the potential of the particle was stable at 6.04 mV. On the other hand, the zeta potential of the liposome before the pullulan acetate-polyethyleneimine conjugate was added was -57.92 mV.

Experimental Example 4: Measurement of stability of tuplesomes

As a result of the measurement using Turbiscan for measuring the stability of the tuplersomes prepared in Example 1, the TSI (Turbiscan Stability Index) value was 0.03, which is 0.5 or less, indicating that the tuplersomes are stable (FIG. 5).

Experimental Example 5: Transdermal absorption promotion effect test

A tuplesome (liposome A) and a general liposome (liposome B) containing 5% glabridin were prepared with the composition shown in Table 19 below, respectively.

Neoderm of Taego Science, an artificial skin, was tested by mounting it in a Franz-type diffusion cell (Lab fine instruments, Korea). The temperature of the Franz-type diffusion cell was set to 33°C, and the solvent of the receptor cell was set to PBS (pH 7.4):ethanol = 1:1 in consideration of the solubility of the hydrophobic substance glabridin. After placing the artificial skin on the chamber and applying 90 mg of the sample to the doner cell, 3 ml of the solvent was extracted from the receptor cell for each time period, and 3 ml of the new solvent was injected. The extracted solvent was mixed with acetonitrile in a 1:1 ratio, and quantitative analysis was performed using HPLC under the following conditions.

- Column: C18 (4.6 × 200 mm, 5 μm)

- Mobile phase: acetonitrile: distilled water = 1:1

- Flow rate: 0.8 ml/min

- Detector: UV 282 nm

The transmittance at 0.5, 1, 2, 3, 4, 5, 6 and 8 hours was calculated by the following formula, and the results are shown in FIG. 6 .

Transmittance = (Transmission amount measured by HPLC (mg) × 3 ml × 2/90 mg) × 100%

As can be seen from FIG. 6 , as a result of application to artificial skin for 8 hours, 2.33% was transmitted in liposome B (general liposome), whereas 3.24% was transmitted in liposome A (tuplesome of the present invention).

Claims (24)

i) active ingredient, hydrogenated lecithin, PEG-5 rapeseed sterol, cholesterol, ceteth-3, ceteth-5, plant sterol and liposomes containing water; and
ii) A triple-layered liposome composition comprising a pullulan acetate-polyethyleneimine conjugate as a coating layer. The method according to claim 1, wherein the liposome contains 0.0005 to 50% by weight of active ingredient, 0.1 to 20% by weight of hydrogenated lecithin, 0.1 to 15% by weight of PEG-5 rapeseed sterol, and 0.01 to 10% by weight of cholesterol. , 0.01 to 5% by weight of Ceteth-3, 0.01 to 5% by weight of Ceteth-5, 0.1 to 15% by weight of plant sterol and 40 to 95% by weight of water Tri-layer liposome composition comprising . The method according to claim 2, wherein the liposome contains 0.0008 to 45% by weight of active ingredient, 0.5 to 18% by weight of hydrogenated lecithin, 0.3 to 12% by weight of PEG-5 rapeseed sterol, and 0.05 to 8% by weight of cholesterol. , 0.05 to 4% by weight of cetes-3, 0.05 to 4% by weight of ceteth-5, 0.5 to 12% by weight of plant sterol, and 43 to 93% by weight of water Tri-layer liposome composition comprising . The method according to claim 3, wherein the liposome contains 0.001 to 40% by weight of active ingredient, 1 to 15% by weight of hydrogenated lecithin, 0.5 to 10% by weight of PEG-5 rapeseed sterol, and 0.1 to 5% by weight of cholesterol. , 0.1 to 3% by weight of Ceteth-3, 0.1 to 3% by weight of Ceteth-5, 1 to 10% by weight of plant sterol and 45 to 90% by weight of water Tri-layer liposome composition comprising . The triple-layered liposome composition according to claim 1, wherein 0.01 to 5 parts by weight of the pullulan acetate-polyethyleneimine conjugate is included with respect to 100 parts by weight of the liposome. The group of claim 1, wherein the active ingredient is a moisturizing agent, whitening agent, wrinkle improving agent, sunscreen agent, hair restorer, vitamin or derivative thereof, amino acid or peptide, anti-inflammatory agent, acne treatment agent, bactericide, female hormone agent, keratin exfoliating agent and natural products. A triple-layered liposome composition, characterized in that at least one selected from 7. The method of claim 6, wherein the humectant is creatine, polyglutamic acid, sodium lactate, hydroproline, 2-pyrrolidone-5-sodium carboxylate, hyaluronic acid, sodium hyaluronate, ceramide, phytosterol, cholesterol, sitosterol, pullulan and proteoglycan. A triple-layered liposome composition, characterized in that at least one selected from the group consisting of. The triple-layered liposome composition according to claim 6, wherein the whitening agent is at least one selected from the group consisting of arbutin and arbutin derivatives, kojic acid, bisabolol, niacinamide, vitamin C and vitamin C derivatives, placenta and allantoin. The triple-layered liposome composition according to claim 6, wherein the anti-wrinkle agent is at least one selected from the group consisting of retinol, retinol derivatives, adenosine, licorice extract, red ginseng extract, and ginseng extract. The triple-layered liposome composition according to claim 6, wherein the sunscreen agent is at least one selected from the group consisting of benzophenone derivatives, para-aminobenzoic acid derivatives, methoxycinnamic acid derivatives, and salicylic acid derivatives. The triple-layered liposome composition according to claim 6, wherein the hair restorer is a blood circulation promoter or a local stimulant. [Claim 12] The triple-layered liposome composition according to claim 11, wherein the blood circulation promoting agent is at least one selected from the group consisting of Monnaceae extract, ceparatin, vitamin E and derivatives thereof, and gamma oryzanol. [Claim 12] The triple-layered liposome composition according to claim 11, wherein the local irritant is at least one selected from the group consisting of red pepper tincture, ginger tincture, cantalis tincture and nicotinic acid benzyl ester. 7. The method according to claim 6, wherein the vitamin or its derivative is vitamin A and its derivatives, vitamins B1, B2, B6, vitamin E and its derivatives, vitamin D, vitamin H, vitamin K, pantothenic acid and its derivatives, biotin, panthenol, and coenzyme. A triple-layered liposome composition, characterized in that at least one selected from the group consisting of Q _{10 and idebenone.} According to claim 6, wherein the amino acid or peptide is cystine, cysteine, methionine, serine, lysine, tryptophan, amino acid extract, epidermal growth factor (EGF), insulin-like growth factor (IGF), fibroblast growth factor (FGF), Cooper tripeptide-1, dipeptide-15, tripeptide-29, tripeptide-1, acetyl hexapeptide-8, nicotinoyl tripeptide-35, hexapeptide-12, hexapeptide-9. Palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, palmitoyl tripeptide-29, palmitoyl tripeptide-1, palmitoyl pentapeptide-3, nonapeptide-7, tripeptide-10 citrulline, sh-polypeptide -15, palmitoyl tripeptide-5, diaminopropioyl tripeptide-33 and r-Spider polypeptide-1 triple-layered liposome composition, characterized in that at least one selected from the group consisting of. [Claim 7] The triple-layered liposome composition according to claim 6, wherein the anti-inflammatory agent is at least one selected from the group consisting of beta-glitylic acid, glitylitanic acid derivative, aminocaproic acid, hydrocortisone, beta-glucan and licorice. [Claim 7] The triple-layered liposome composition according to claim 6, wherein the acne treatment agent is at least one selected from the group consisting of estradiol, estrogen, ethynyl estrariol, tricroic acid, and azelic acid. [Claim 7] The triple-layered liposome composition according to claim 6, wherein the biocide is at least one selected from the group consisting of benzalkonium chloride, benzethonium chloride and halocalban. The triple-layered liposome composition according to claim 6, wherein the female hormone agent is at least one selected from the group consisting of estradiol, ethynyl estradiol and isoflavones. The triple-layered liposome composition according to claim 6, wherein the exfoliating agent is at least one selected from the group consisting of sulfur, salicylic acid, AHA, BHA and resorcin. The method according to claim 6, wherein the natural product is a fern extract, clown beard extract, white flower extract, rhubarb extract, licorice extract, aloe extract, chamomile extract, rosehip extract, ginkgo leaf extract, horse chestnut extract, ginseng extract, loofah extract, cucumber extract, seaweed. A triple-layered liposome composition, characterized in that at least one selected from the group consisting of extract, seaweed extract, kelp extract, hemp extract, snail extract, young woman extract, hinokitiol and beta-carotene. The method according to claim 1, wherein the plant sterols are rapeseed sterol, avocadosterol, PEG-15 phytosterol, acifam sterol, phytosteryl macadamate, soysterol, dolconsterol, canolasterol, rice bran sterol and pomegranate. A triple-layered liposome composition, characterized in that at least one selected from the group consisting of sterols. A cosmetic composition comprising the triple-layered liposome composition of any one of claims 1 to 22. The cosmetic composition according to claim 23, comprising 1 to 60 wt% of the triple-layered liposome composition.

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