KR20210103813A - Triple layered core-shell capsule and method for preparing the same - Google Patents

Abstract

According to the specification, disclosed are a triple layer core-shell capsule containing no plastic and a method for manufacturing the same. In one aspect, the capsule of the present disclosure is made of a biopolymer and has an eco-friendly character because the capsule does not use plastic materials that are known to cause environmental disturbance, for example, PMMA. In one aspect, the capsule of the present disclosure has ease of transport and use by supporting a liquid material by controlling one-time usage of the liquid material that is difficult to transport, and the capsule of the present disclosure can be burst with grip force, thereby being used in the form of a squeeze. Moreover, in one aspect, since the capsule of the present disclosure has pH sensitivity and temperature sensitivity, the capsule is completely decomposed within 40 hours just by being thrown into a toilet after use, thereby making post-treatment easy.

Description

Triple layered core-shell capsule and method for preparing the same

Disclosed herein are a triple-layered core-shell capsule containing no plastic and a method for manufacturing the same.

In most conventional product technologies, polymethyl methacrylate (PMMA, polymethyl methacrylate), acrylate synthetic polymer, PEG-based material, etc. have been mainly used for the form of carriers carrying internal liquid active ingredients and stability of the formulation. The macro carrier produced in this way does not decompose within a few days and there is a problem that plastic residues remain, and the problem that microplastics such as PMMA released as residues cause ecological disturbance is becoming an issue. Although many eco-friendly carriers have been developed, the addition of synthetic polymers such as acrylate or PEG is inevitable for formulation stability, so there are still issues of environmental and human safety risks.

Accordingly, the present inventors have been conducting research to develop a carrier that sufficiently secures stability without using a microplastic material that causes ecosystem disturbance, a triple-layered core-shell containing pectin, alginate, and chitosan of the present disclosure The capsule was completed.

KR Patent Publication No. 10-2010-0040864 (2010.04.21.)

In one aspect, an object of the present disclosure is the core is a liquid composition, the shell is a first shell layer consisting of a pectin membrane surrounding the core; and a second shell layer surrounding the first shell layer, wherein the second shell layer is a core-shell capsule containing alginate and chitosan and a method for manufacturing the same.

In order to achieve the above object, the present disclosure in one aspect is a core-shell capsule, wherein the core is a liquid composition, the shell is a first shell layer consisting of a pectin membrane surrounding the core; and a second shell layer surrounding the first shell layer, wherein the second shell layer includes alginate and chitosan.

In one aspect, the present disclosure provides a method for preparing a liquid marble by dropping a liquid composition on a pectin powder; forming an alginate film surrounding the liquid marble by treating the liquid marble with an alginate solution; and forming a chitosan film surrounding the alginate film by treating the chitosan solution on the liquid marble surrounded by the alginate film.

In one aspect, the capsule of the present disclosure is made of a biopolymer, and does not use a plastic material known to cause environmental disturbance, for example, PMMA, and has an eco-friendly nature. In one aspect, the capsule of the present disclosure has ease of transport and use by controlling and supporting the one-time usage of a liquid material that is difficult to transport, and since the capsule of the present disclosure can be popped with a grip force, it can be used in a squeezed form. Moreover, since the capsule of the present disclosure in one aspect has pH sensitivity and temperature sensitivity, it is completely decomposed within 40 hours just by throwing it into the toilet after use, thereby making post-treatment easy.

1 is a view showing the results of observing the appearance of the triple-layer core-shell capsule of the present disclosure.
2 is a diagram schematically illustrating a process for manufacturing a triple-layer core-shell capsule of the present disclosure.
3 is a diagram showing the results of observing the appearance of the three-layered core-shell capsule of the present disclosure prepared by varying the volume of the supported emulsion to 1 μl, 5 μl, 10 μl, 50 μl, and 100 μl, respectively.
4 is a view showing the result of confirming the change of the triple-layer core-shell capsule of the present disclosure according to the grip force.
5 is a view showing the results of confirming the stability of the triple-layer core-shell capsule of the present disclosure.
6 is a diagram showing the results of confirming the stability and structural change of the triple-layered core-shell capsule of the present disclosure prepared by varying the treatment concentrations of the alginate solution and the chitosan solution during manufacture.
7 is a view showing the results of confirming the pH sensitivity of the triple-layer core-shell capsule of the present disclosure.
8 is a view showing the results of confirming the pH sensitivity of the triple-layer core-shell capsule of the present disclosure.
9 is a view showing the result of confirming the temperature sensitivity of the triple-layer core-shell capsule of the present disclosure.
10 is a view showing the result of confirming the pH sensitivity of the lyophilized triple-layer core-shell capsule of the present disclosure.

Hereinafter, it will be described in detail.

In one aspect, the present disclosure provides a core-shell capsule, wherein the core is a liquid composition, and the shell includes a first shell layer comprising a pectin membrane surrounding the core; and a second shell layer surrounding the first shell layer, wherein the second shell layer includes alginate and chitosan.

In one embodiment, the triple layer refers to a capsule composed of a core, a first shell layer, and a second shell layer in a capsule having a core-shell structure.

In one embodiment, 'capsule' refers to a closed container.

Alginate and chitosan of the second shell layer form a mixed structure through electrostatic attraction (coacervation), the ratio of the alginate component is higher toward the inside, and the ratio of the chitosan component is higher toward the outside. are doing Accordingly, in one embodiment, the concentration of alginate of the second shell layer decreases while having a continuous concentration gradient toward the outside, and the concentration of chitosan in the second shell layer has a continuous concentration gradient toward the outside. increases

In one embodiment, the thickness of the first shell layer may be 50 μm to 100 μm.

In one embodiment, the thickness of the second shell layer may be 200 μm to 50 mm.

In one embodiment, the diameter of the capsule is 1 mm or more, 1.5 mm or more, 2 mm or more, 2.5 mm or more, 3 mm or more, 3.5 mm or more, 4 mm or more, 4.5 mm or more, 5 mm or more, 5.5 mm or more, 6 mm or more, 6.5 mm or more, 7 mm or more, 7.5 mm or more, 8 mm or more, 8.5 mm or more, 9 mm or more, 9.5 mm or more, 10 mm or more, 10.5 mm or less, 11 mm or less, 11.5 mm or less, 12 mm or less , 12.5 mm or less, 13 mm or less, 13.5 mm or less, 14 mm or less, 14.5 mm or less, 15 mm or less, 15.5 mm or less, 16 mm or less, 16.5 mm or less, 17 mm or less, 17.5 mm or less, 18 mm or less, 18.5 mm or less, 19 mm or less, 19.5 mm or less, 20 mm or less, preferably 1 mm to 20 mm, more preferably 1.5 mm to 6 mm.

In one embodiment, the liquid composition may be included without limitation as long as it is a material in a liquid state, and preferably any material that can form a liquid marble together with pectin or can be replaced with the formed liquid marble may be included without limitation. , For example, it may be a gel, cream, emulsion composition, water dispersion composition or oil dispersion composition. For example, the liquid composition may be at least one of a cosmetic composition, a food composition, and a pharmaceutical composition. For example, the pharmaceutical composition may include cells, DNA, RNA, and the like.

In one embodiment, the liquid composition is 1 μl or more, 2 μl or more, 3 μl or more, 4 μl or more, 5 μl or more, 6 μl or more, 7 μl or more, 8 μl or more, 9 μl or more, 10 μl or more, 11 μl or more, 12 μl or more, 13 μl or more, 14 μl or more, 15 μl or more, 16 μl or more, 17 μl or more, 18 μl or more, 19 μl or more, 20 μl or more, 30 μl or more, 40 μl or more, 50 μl or more, 60 μl or more, 70 μl or more, 80 μl or more, 90 μl or more, 100 μl or more, 110 μl or more, 120 μl or more, 130 μl or more, 140 μl or more, 150 μl or more, 160 μl or more, 170 μl or more, 180 μl or more, 190 μl or more, 200 μl or more, 210 μl or less, 220 μl or less, 230 μl or less, 240 μl or less, 250 μl or less, 260 μl or less, 270 μl or less, 280 μl or less, 290 μl or less, 300 μl or less, 310 μl or less, 320 μl or less, 330 μl or less, 340 μl or less, 350 μl or less, 360 μl or less, 370 μl or less, 380 μl or less, 390 μl or less, 400 μl or less, 410 μl or less, 420 μl or less, 430 μl or less, 440 μl or less, 450 μl or less, 460 μl or less, 470 μl or less, 480 μl or less, 490 μl or less, 500 μl or less, preferably in a volume of 1 μl to 200 μl can

In one embodiment, the capsule is at room temperature at least 0.5 kPa, at least 0.6 kPa, at least 0.7 kPa, at least 0.8 kPa, at least 0.9 kPa, at least 1 kPa, at least 1.1 kPa, at least 1.2 kPa, at least 1.3 kPa, at least 1.4 kPa, 1.5 It bursts by pressure of kPa or more, 1.6 kPa or more, 1.7 kPa or more, 1.8 kPa or more, 1.9 kPa or more, 2.0 kPa or more.

In one embodiment, the capsule has pH susceptibility.

In one example, the capsule is stored in an aqueous solution having a pH of 7.4 at room temperature and atmospheric pressure for more than 7 hours, 8 hours or more, 9 hours or more, 10 hours or more, 11 hours or more, 12 hours or more, 13 hours or more, 14 hours or more, 15 hours or more. or more, 16 hours or more, 17 hours or more, 18 hours or more, 19 hours or more, 20 hours or more, 21 hours or more, 22 hours or more, 23 hours or more, 24 hours or more, 25 hours or more, 26 hours or more, 27 hours or more, 28 hours or more, 29 hours or more, 30 hours or more, 31 hours or more, 32 hours or more, 33 hours or more, 34 hours or more, 35 hours or more, 36 hours or more, 37 hours or more, 38 hours or more, 39 hours or more, or 40 hours or more. In the above, the liquid composition in the capsule may be released.

For example, the capsules are stored in an aqueous solution having a pH of 7.4 at room temperature and atmospheric pressure for 40 hours or less, 39 hours or less, 38 hours or less, 37 hours or less, 36 hours or less, 35 hours or less, 34 hours or less, 33 hours or less, 32 hours or less. or less, 31 hours or less, 30 hours or less, 29 hours or less, 28 hours or less, 27 hours or less, 26 hours or less, 25 hours or less, 24 hours or less, 23 hours or less, 22 hours or less, 21 hours or less, 20 hours or less, It may be completely decomposed in 19 hours or less, 18 hours or less, 17 hours or less, 16 hours or less, 15 hours or less, or 14 hours or less. For example, the 'completely decomposed' refers to a state in which the shape of the capsule cannot be confirmed because the binding of the structure is divided into capsules made of a natural biodegradable composition in which nano-scale microplastic particles are not likely to remain.

In one embodiment, the capsule is one having a temperature susceptibility (tempo-susceptibility). For example, the capsule may have a thickness of 271.10 μm to 348.32 μm of the second shell layer when 15 hours have elapsed in an aqueous solution having a room temperature, atmospheric pressure, and pH of 7.4.

In one embodiment, the capsule may be lyophilized. In this case, for example, the liquid composition in the capsule may also be freeze-dried.

In one embodiment, the capsule may be one or more of a cosmetic delivery system, a food delivery system, and a drug delivery system.

In one aspect, the present disclosure provides a method for preparing a liquid marble by dropping a liquid composition on a pectin powder; forming an alginate film surrounding the liquid marble by treating the liquid marble with an alginate solution; and forming a chitosan film surrounding the alginate film by treating the chitosan solution on the liquid marble surrounded by the alginate film.

In one embodiment, the liquid marble refers to droplets that do not have adhesiveness. In one embodiment, the liquid marble has elasticity, but does not agglomerate into a larger mass when lightly pressed. Also, in one example, the liquid marble is stable on solid and liquid surfaces.

In one embodiment, the treatment concentration of the alginate solution and the treatment concentration of the chitosan solution are 0.3 or more, 0.4 or more, 0.5 or more, 0.6 or more, 0.7 or more, 0.8 or more, 0.9 or more, 1 or more, 1.1 or less, 1.2 or less, 1.3 or less, 1.4 or less, 1.5 or less, 1.6 or less, 1.7 or less, 1.8 or less, 1.9 or less, 2 or less, 2.1 or less, 2.2 or less, 2.3 or less, 2.4 or less, preferably alginate The treatment concentration and the treatment concentration of the chitosan solution may have a weight ratio of 1: 0.3 to 2.4, and more preferably, have a weight ratio of 1:0.5 to 2.

Redundant content is omitted in consideration of the complexity of the present specification, and terms not defined otherwise in the present specification have the meanings commonly used in the technical field to which the present disclosure pertains. In addition, 'or' used in this specification is a concept including 'and', unless explicitly stated to the contrary.

Hereinafter, examples and experimental examples will be described in detail to help the understanding of the present disclosure. However, the following Examples and Experimental Examples are merely illustrative of the contents of the present disclosure, and the scope of the present disclosure is not limited to the following Examples and Experimental Examples. Examples and experimental examples of the present disclosure are provided to more completely explain the present disclosure to those of ordinary skill in the art.

Example 1. Preparation of Triple Layer Core-Shell Capsule-1

Liquid marble was prepared by dropping 1-200 μl of PBS (phosphate buffer saline), a liquid emulsion, on pectin powder (Signa-aldrich) to form a powdery thin film (first shell layer) (Liquid marble technology). Then, liquid (oil) marble was added to a 2% (w/v) alginate solution (Wako) to form an alginate surface film, which was then added to a 2% (w/v) chitosan solution (Sigma-aldrich) to give a negative charge. formed a chitosan film through electrostatic coacervation between alginate and positively charged chitosan (second shell layer). After that, it was soaked in secondary distilled water to wash the remaining residual chitosan and alginate without coacervation. After the washing was completed, it was dried at room temperature until the maximum amount of moisture on the surface of the membrane was removed to obtain a capsule containing the emulsion. The result of observing the appearance of the finally manufactured capsule is shown in FIG. 1 , and the series of manufacturing processes are schematically shown in FIG. 2 .

Example 2. Triple Layer Core-Shell Capsule Preparation-2

A triple-layered core-shell capsule was prepared in the same manner as in Example 1, except that a blue dye (Trypan blue (Sigma Aldrich)) was mixed with the emulsion when preparing liquid marble.

Experimental Example 1. Confirmation of size change of triple-layered core-shell capsules prepared according to the volume of the supported emulsion

It was confirmed whether the size of the finally prepared triple-layered core-shell capsule was controlled by controlling the volume of the supported emulsion. For this, a triple-layered core-shell capsule was prepared in the same manner as in Example 1, except that PBS as a liquid emulsion was added in volumes of 1 μl, 5 μl, 10 μl, 20 μl, and 50 μl, respectively. Meanwhile, a double-layered core-shell capsule was prepared in the same manner as in Example 1, except that PBS as a liquid emulsion was added in volumes of 100 μl, 200 μl, and 500 μl, respectively, and a pectin powdery thin film was not formed. The results of observing the appearance of the prepared capsules are shown in FIG. 3 . As can be seen in FIG. 3 and Table 1 below, it was confirmed that as the volume of the supported liquid emulsion increased, the size of the finally prepared triple-layer or double-layer core-shell capsule gradually increased.

Liquid emulsion volume (μl) One 5 10 20 50 100 200 500 of the capsules
Diameter (mm) 1.78 ± 0.14 2.85 ± 0.01 3.60 ± 0.12 4.13 ± 0.19 5.83 ± 0.21 8.56 ± 0.15 10.98 ± 0.94 14.86 ± 1.37

Experimental Example 2. Confirmation of change in triple-layer core-shell capsule according to grip force

Three healthy women in their 30s at room temperature applied strong pressure (about 1.1 kPa at ^{1 s −1} frequency) to the triple-layered core-shell capsule prepared in Example 1 by hand. As a result, as can be seen in FIG. 4, the capsule bursts with the grip force, which means that the triple-layered core-shell capsule of the present disclosure is transported in the form of a capsule and the user squeezes it by hand to use the supported liquid material. suggest

Experimental Example 3. Confirmation of stability of triple-layer core-shell capsules

In order to confirm the stability of the triple-layered core-shell capsule of the present disclosure, a double-layered pectin capsule (Comparative Example 1) and a triple-layered, but not containing chitosan, pectin-alginate capsule (Comparative Example 2) were prepared. In Comparative Example 1, a blue dye (Trypan blue (Sigma Aldrich)) was mixed with 150 μl of PBS, which is a liquid emulsion, and then dropped on pectin powder (Signa-aldrich) to form a powdery thin film to prepare liquid marble, and comparison In Example 2, a blue dye (Trypan blue (Sigma Aldrich)) was mixed with 150 μl of PBS, which is a liquid emulsion, and then dropped on pectin powder (Signa-aldrich) to form a powdery thin film. ) was prepared by putting marble in a 2% (w/v) alginate solution (Wako) and forming an alginate surface film. On the other hand, it was attempted to prepare a capsule containing only chitosan in the shell, but in this case, the capsule was not formed in the first place.

The three-layered core-shell capsules of Example 2 and the capsules of Comparative Examples 1 and 2 were then placed at room temperature and atmospheric pressure for 30 minutes. The results are shown in FIG. 5 .

As can be seen in FIG. 5 , the triple-layered core-shell capsule of the present disclosure (Example 2) was stable without breaking its structure even after several minutes and several tens of hours. However, in the double-layered pectin capsule (Comparative Example 1), the capsule structure was broken within minutes after manufacture, and the triple-layered pectin-alginate capsule (Comparative Example 2), which does not contain chitosan, had the capsule structure broken within minutes. . This indicates that the triple-layer core-shell capsule of the present disclosure is a capsule in which structural stability is secured.

Experimental Example 4. Confirmation of stability and structural change of triple-layer core-shell capsules prepared by different treatment concentrations of alginate solution and chitosan solution

When the three-layer core-shell capsules were formed, the stability and structural changes of the capsules were confirmed when the treatment concentrations of the alginate solution and the chitosan solution were different. For this, a triple-layered core-shell capsule (Comparative Example 3) in the same manner as in Example 1, except that a 2% (w/v) concentration of alginate solution was treated and a 5% (w/v) concentration of chitosan solution was treated. , the left capsule in Figure 6) was prepared, in the same manner as in Example 1, except that 5% (w/v) concentration of alginate solution was treated and 2% (w/v) concentration of chitosan solution was treated. A middle layer core-shell capsule (Comparative Example 4, right capsule in FIG. 6 ) was prepared. Thereafter, the structures of the capsules of Comparative Examples 3 and 4 prepared using a microscope (Nikon Eclipse Ti-S; Nikon, Tokyo, Japan) were confirmed. The results are shown in FIG. 6 .

As shown in FIG. 6, the capsule of Example 1, in which the ratio of the treated alginate solution and the chitosan solution converges to 1:1, has a first shell layer made of a pectin membrane, an alginate layer and a second shell layer made of chitosan 50 each While maintaining the thickness of μm and 500 μm, it formed a strong triple layer. However, in Comparative Examples 3 and 4 prepared by treating the alginate solution and the chitosan solution in a weight ratio of 2:5 and 5:2, respectively, the polymers penetrated the inside of the capsule and the triple layer was broken. This indicates that the triple-layer core-shell capsule of the present disclosure is a capsule in which structural stability is secured.

Experimental Example 5. Confirmation of pH Sensitivity of Triple Layer Core-Shell Capsule

5-1. Confirmation of pH Sensitivity of Triple-Layered Core-Shell Capsule Containing Colorless Emulsion

In order to check whether the triple-layer core-shell capsule of the present disclosure has pH sensitivity, several capsules of Example 1 were placed in distilled water with different pHs of 1.5, 2.1, 5.2, 7.4, and 9, respectively, and Changes were observed visually. At this time, this experiment was performed at room temperature and atmospheric pressure conditions. The results are shown in FIG. 7 . As can be seen in FIG. 7 , it was confirmed that in the case of a weak base or neutral pH, for example, a pH value of 7.4, decomposition occurs after a certain period of time. This indicates that the present capsule has pH sensitivity.

5-2. Confirmation of pH Sensitivity of Triple-Layered Core-Shell Capsule Containing Blue Emulsion

In order to more closely check whether the triple-layer core-shell capsule of the present disclosure has pH sensitivity, several capsules of Example 2 were placed in distilled water with different pH values of 2.54, 5.3, 7.4, and 8.54, respectively, and the time The change of the capsule was observed with the naked eye. At this time, this experiment was performed at room temperature and atmospheric pressure conditions. The results are shown in FIG. 8 . As can be seen in FIG. 8 , it is possible to control the release of the internal support material with a time difference in various pH ranges.

As can be seen through the above series of experiments, this capsule is a plastic-free carrier material capable of pH-sensitive sustained-release release, and it is decomposed by throwing the capsule into neutral or weakly basic water, for example, by throwing it in a toilet bowl, etc. This suggests that the capsules have the advantage of being easy to handle after use.

Experimental Example 6. Confirmation of temperature sensitivity of triple-layer core-shell capsules

In order to check whether the triple-layer core-shell capsule of the present disclosure has temperature sensitivity, the temperature of the capsule of Example 2 was changed to room temperature (about 25° C.) and 37° C., respectively, and then the change of the capsule was measured 15 time was observed (at atmospheric pressure). At this time, observation was performed in distilled water with pH 5.4, 7.4, and 8.8, and the outermost shell of the capsule was enlarged and observed using a microscope (Nikon Eclipse Ti-S; Nikon, Tokyo, Japan). The results are shown in FIG. 9, and in particular, the thickness measurement results of the second shell in the capsule are shown in Table 2 below.

Thickness of the second shell (μm) Room temperature (about 25 degrees Celsius) 37℃ pH 5.4 pH 7.4 pH 8.8 pH 5.4 pH 7.4 pH 8.8 1 hours 258.95 ± 4.42 312.04 ± 24.24 684.54 ± 12.02 283.84 ± 6.95 349.29 ± 5.59 679.75 ± 13.53 15 hours 370.93 ± 63.03 309.71 ± 38.61 840.62 ± 19.66 232.97 ± 7.65 522.09 ± 18.32 1174.55 ± 16.72

As can be seen in FIG. 9 and Table 2, the membrane structure of the capsule was loosened and stretched at 37° C., which is similar to the body temperature, unlike at room temperature. That is, it was confirmed that it has temperature sensitivity. This generally shows a morphological and functional difference different from other alginate beads that do not exhibit temperature sensitivity, and such temperature sensitivity has the advantage of increasing the delivery rate of capsule contents when applied to a living body of a capsule carrying a drug or the like.

Experimental Example 7. Confirmation of pH sensitivity of lyophilized triple-layer core-shell capsules

In order to determine whether the pH sensitivity of the present capsule changes when freeze-drying the capsule having the triple-layered core-shell structure of the present disclosure, first, the triple-layered core-shell capsule prepared in Example 1 was completely prepared at -80°C. After freezing, it was freeze-dried using a lyophilizer (IlShinBioBase) at -88°C and 5 m Torr gauge for one day so that the liquid composition inside could be freeze-dried. Then, several lyophilized capsules were placed in distilled water having a pH of 1.5, 2.1, 5.2, 7.4, and 9, respectively, and changes in the capsules over time were visually observed. The results are shown in FIG. 10 . As shown in FIG. 10 , using the triple-layer core-shell capsule of the present disclosure, it was possible to prepare a delivery system carrying an active ingredient inside in a shape in which the freeze-dried form was maintained. In addition, similar to the case of not freeze-drying, it was rapidly decomposed within 40 hours at a pH above neutral.

Claims (17)

A core-shell capsule comprising:
The core is a liquid composition,
The shell may include a first shell layer comprising a pectin film surrounding the core; and
a second shell layer surrounding the first shell layer;
The second shell layer comprises alginate and chitosan, the capsule. According to claim 1,
The concentration of alginate of the second shell layer decreases while having a continuous concentration gradient toward the outside, and the concentration of chitosan in the second shell layer increases while having a continuous concentration gradient toward the outside. According to claim 1,
The thickness of the first shell layer is 50 μm to 100 μm capsule. According to claim 1,
The thickness of the second shell layer is 200 μm to 50 mm capsule. According to claim 1,
The capsule has a diameter of 1 mm to 2 cm. According to claim 1,
The liquid composition is a gel, cream, emulsion composition, an aqueous dispersion composition, or an oil dispersion composition. According to claim 1,
The liquid composition is a capsule at least one of a cosmetic composition, a food composition, and a pharmaceutical composition. According to claim 1,
The capsule will burst by a pressure of 0.5 kPa or more at room temperature. According to claim 1,
The capsule is a capsule having a pH sensitivity (pH susceptibility). 10. The method of claim 9,
The capsule is a capsule in which the liquid composition in the capsule is released when it exceeds 7 hours in an aqueous solution having a pH of 7.4 at room temperature and atmospheric pressure. 10. The method of claim 9,
The capsule is a capsule that is completely decomposed in 40 hours or less in an aqueous solution having a pH of 7.4 at room temperature and atmospheric pressure. According to claim 1,
The capsule is a capsule having a temperature susceptibility (tempo-susceptibility). 13. The method of claim 12,
When the capsule is in an aqueous solution of room temperature, atmospheric pressure, and pH of 7.4 for 15 hours, the thickness of the second shell layer is 271.10 μm to 348.32 μm. According to claim 1,
The capsule is a lyophilized capsule. According to claim 1,
The capsule is at least one of a cosmetic carrier, a food carrier, and a drug carrier. Dropping the liquid composition on the pectin powder to prepare a liquid marble (liquid marble);
forming an alginate film surrounding the liquid marble by treating the liquid marble with an alginate solution; and
16. A method of manufacturing a core-shell capsule according to any one of claims 1 to 15, comprising the steps of: forming a chitosan film surrounding the alginate film by treating the chitosan solution on the liquid marble surrounded by the alginate film. 17. The method of claim 16,
The treatment concentration of the alginate solution and the treatment concentration of the chitosan solution 1: How to have a weight ratio of 0.3 to 2.4.

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