KR20210058550A - Intermediate Parent for improvement of hilum color of soy-paste bean varieties, and breeding method thereof - Google Patents

Abstract

The present invention relates to an intermediate parent for improving a hilum color of soybean paste bean varieties and a breeding method thereof and, more specifically, to an HBC_2 Daepung 0723-based plant, which is an intermediate parent for improving a hilum color of soybean paste bean varieties and medium-sized bean varieties and a breeding method thereof. According to the present invention, in comparison with the existing breeding through backcrossing which takes more than 6 to 7 generations, since the present invention uses molecular markers together to use an excellent intermediate parent, individuals can be selected from the second generation of backcrossing at the earliest, so that a period of breeding new varieties can be reduced and effort and cost required for crop improvement can be reduced, thereby providing an advantage of increasing the scale and efficiency of breeding. In addition, HBC_2 Daepung 0723 series produced by the present invention does not have a hilum color while having similar yield characteristics to the existing Daepung variety, thereby being expected to be usefully utilized in improving soybean paste bean varieties.

Description

[Intermediate Parent for improvement of hilum color of soy-paste bean varieties, and breeding method thereof]

The present invention relates to an intermediate model for improving the coloration of soybean varieties for soybeans and a method for breeding the same, and to breed HBC ₂ Daepoong0723 lineage plants, which is an intermediate model for color improvement of soybean varieties for soybeans and mesophilic varieties, and breeding the lineage plants. It's about the method.

Soybeans are a very important crop in the world economically, and are widely used for food and feed that humans always consume, and in recent years, many studies have been conducted as a raw material for biodiesel. Therefore, many researchers around the world are concentrating on cultivar development and breeding while discovering and researching numerous useful genes in soybeans.

In the food industry, soybeans are used for a variety of purposes, especially in Korea, mainly used for soybean paste and tofu. Soybean varieties suitable for the above use should be used, and varieties are usually selected only with the phenotype of beans, and traits used for this include the size of seeds, color of seed coat, and color. In general, varieties with high protein content, large seeds, and colorless (yellow) are treated as good varieties. For example,'Daewon Bean' has a large seed and high quality, so consumers prefer it for soy sauce and tofu, but it has the disadvantage of being vulnerable to diseases such as fire blight, purpura, and black root rot. On the other hand, varieties such as'Daepung' and'Shin Paldal No. 2'are strong against diseases such as SMV, fire blight, and black root rot, and their cultivation stability is high, so their yield is very good. There is a problem.

On the other hand, the current soybean breeding program is largely operated in 4 groups: for soybean paste and tofu, for namul, for rice with a focus on black beans, and for short-term growth. Soybeans for soybean paste and tofu, which have the largest consumption of more than 70% in Korea, are promoting variety development in two directions. In other words, the development of varieties with thick bean grains and excellent appearance quality favored by former owners, as well as the development of multiple varieties adapted to large-scale mechanized cultivation. The grown soybean varieties contain a unique crude product species region within the chromosome through the process of cross breeding. As this area goes through a process of reshuffling through a continuous breeding process, a pattern peculiar to the cultivar emerges.

Currently, the female breeding method is mainly used as a breeding method used to improve the shortcomings of the cultivar or to introduce only the desired trait. The most important factor when developing a customized variety using this breeding method is to accurately introduce only the region containing the target trait in the donor parent into the recurrent parent. This is because if the region to be introduced is large, there is a high possibility of linkage drag in which genes of other traits are introduced together.

Therefore, the present inventors used the full-length genome information of williams82, a standard genome cultivar, and five varieties including Baegunkong, Shinpaldalkong No.2, Shingi, Daepung, and Golden Bean in the previous study, and finally discovered based on the result of searching for the region of mutation in the genome. Using one 202 insertion/deletion (Indel) markers (Sohn et al., 2017, Front.Plant Sci. 8:520), we developed a soybean breeding method that shortens the number of crossbreeding while introducing the relevant genetic domain to a minimum. And, using this, an intermediate model line that can improve the color of soybean varieties for soybeans and mesophilic varieties was established.

As described above, the present inventors have developed HBC, an excellent intermediate model having disaster resistance, high yield, and pale yellow color through a genomics-assisted breeding process using a molecular breeding platform based on a mutation region developed for each soybean variety. _{2 The} Daepung0723 system was established, and the present invention was completed based on this.

Accordingly, an object of the present invention is to provide a _{HBC 2} Daepung 0723 lineage plant for improving color production of soybean varieties deposited with the registration number KACC 98065P to the National Academy of Agricultural Sciences Agricultural Genetic Resource Center.

In addition, another object of the present invention is _{to provide a seed according to the HBC 2} Daepung0723 lineage plant and a progeny of the seed.

In addition, it is another object of the _{present invention to provide a method for breeding the HBC 2 Daepung0723 lineage plants.}

However, the technical problem to be achieved by the present invention is not limited to the problems mentioned above, and other problems that are not mentioned will be clearly understood by those skilled in the art from the following description.

_{In order to achieve the object of the present invention as described above, the present invention provides a HBC 2} Daepung 0723 lineage plant for improving the color of soybean varieties deposited with the registration number KACC 98065P to the National Institute of Agricultural Sciences Agricultural Genetic Resource Center.

In addition, the present invention provides _{a seed according to the HBC 2} Daepung0723 lineage plant and a progeny of the seed.

In one embodiment of the present invention, the beans may be for soybeans and mesophilic species.

In another embodiment of the present invention, the color may be yellow.

In still another embodiment of the present invention, the HBC ₂ Daepung0723 lineage plant may have a white grain weight of 23 to 26 g.

In another embodiment of the present invention, the HBC ₂ Daepung0723 lineage plant may have an average length of 57 to 62 cm, and an average narrow number of 22 to 26.

In addition, the present invention provides a _{method for breeding the HBC 2} Daepung 0743 lineage plants comprising the following steps.

_{(a) cultivating a hybrid (F 6} ) by artificial crossing using Daepung bean as a crossing model and golden bean as a crossing copy, and then selecting a line in which the target region corresponding to the color-making-related locus is minimally introduced;

_{(b) performing cross-breeding twice using the plant (BC 1} F ₂ ) obtained by sowing the selected lineage plant as a model, and using Daepung bean as a backup;

(c) selecting a plant having the highest similarity to Daepung from among the plants obtained after the overcrossing; And

(d) obtaining seeds from the selected plants and developing them to select a line whose color is yellow in _{BC 2} F _{2 generation.}

As an embodiment of the present invention, in step (a), the step of selecting a lineage in which a target region corresponding to a color-removal-related locus has been introduced at a minimum may be performed through next-generation sequencing (NGS).

In another embodiment of the present invention, in step (c), selecting a plant having the highest similarity to Daepung is a single base variation (SNV) dense region using full-length genome information obtained through next-generation sequencing (NGS). It may be based on information on the entire mutation area analyzed for.

In still another embodiment of the present invention, the HBC ₂ Daepung0723 lineage plant may have a color-related genetic region of 2.45 Mb inserted therein.

According to the present invention, compared to the conventional breeding through crossbreeding takes 6-7 generations or more, the present invention uses an excellent intermediate model by using a molecular marker together, so that individuals can be selected from the second generation of crossbreeding as soon as possible. It has the advantage of shortening the period for cultivating new varieties, and increasing the scale and efficiency of breeding by reducing the effort and cost required for crop improvement. In addition, the HBC ₂ Daepung0723 line produced by the present invention is expected to be useful in improving soybean varieties for soybeans because the yield characteristics are similar and no color production compared to the existing Daepung, which is a conventional variety.

FIG. 1 shows the structure of chromosome 1 of 24 soybean varieties by analyzing a variation block (VB).
Figure 2 is a result of analyzing the similarity between varieties based on the region of mutation in order to select a mating parent varieties for improving the color of Daepung bean.
3 is a diagram showing the process of fostering the _{HBC 2} Daepung0723 system, which is an intermediate model according to the present invention.
4 is a table showing the similarity of each chromosome between the breeding line and the Daepung variety.
5 is a diagram showing the degree of similarity of chromosomes using full-length genome information (blue: dafeng, red: golden, yellow: hetero).
6 is a result of confirming the degree of insertion of a color-relevant genetic region into _{HBC 2 Daepung0723 line through chromosome resequencing.}
Figure 7 shows the length (cm), 100 grains (g), and yield (g/individual) of growing line plants, including _{HBC 2} Daepung 0923, which is a middle model for improving the coloration of mesophilic and mesophilic varieties, Daepung and Golden varieties It is the result shown in comparison with.

The present inventors use the breeding method, which is a breeding method that is used to improve the shortcomings of the variety or introduce only the desired trait, while using the molecular breeding platform based on the mutation region developed for each soybean variety to minimize the related genetic region. During the introduction, a bean breeding method was developed to shorten the number of crossbreeding, and an intermediate seedling system was established that could improve the coloration of namul and full-grown soybean varieties.

_{Accordingly, the present invention provides HBC 2} Daepung 0923 lineage plants for improving color production of soybean varieties deposited with the registration number KACC 98065P to the National Academy of Agricultural Sciences Agricultural Genetic Resource Center.

In addition, the present invention provides a seed according to _{the HBC 2 Daepung0723 lineage plants.}

In addition, the present invention provides the offspring of seeds according _{to the HBC 2 Daepung0723 lineage plants.}

The present inventors secured an intermediate parent plant of _{the HBC 2} Daepung 0723 line through a genomics-assisted breeding method using a molecular breeding platform based on a mutation region as follows.

The term “intermediate mother” used in the present invention refers to a higher-generation line that is not used as a cultivar but is improved to a certain level and used as a mating parent.

The method developed in the present invention selects the most genetically similar breed through genome analysis in the selection process of the mating and mating copies compared to the conventional method, and the mutation region of the smallest size containing the target trait in the _{F 2 isolated generation.} Individuals with (VB) can be selected, and through this, excellent intermediate seedling plants having disaster resistance, high yield, and pale yellow color were obtained.

More specifically, in one embodiment of the present invention, a breed that is strong against diseases and has high cultivation stability and has excellent yield, but has a low preference due to brown color, is used as a breeding model, and a breed that is genetically most similar thereto is used. In order to select, the full-length genome information of 24 soybean varieties was obtained through next-generation sequencing analysis. Based on this, the single base variation (SNV) cluster area was analyzed to search the entire variation area of each of 24 varieties, and the similarity between varieties was analyzed based on the divided variation block. As a result, gold, which had the highest genetic similarity to Daepung and excellent in color-free seed-out appearance characteristics, was selected as the mating copy (see Example 1).

In another embodiment of the present invention, in accordance with the process shown in FIG. 3, a middle model was grown using Daepung beans as a mating model and golden beans as a mating copy. _{Briefly, among the lines obtained through the Daepung/Golden recombination, 6} lines of HD-487-F with the minimum target region corresponding to the color-making-related locus were selected, and the plant of the selected line was selected as a model, and Daepung bean was a copy. As a result, female crossings were carried out twice. After that, one individual having a high similarity to Daepung is selected from the individuals obtained through the two crossbreedings, and after securing a plant that has been confirmed to be crosscrossed twice with Daepung, the plant with the highest similarity to Daepung is selected and the seeds thereof. _{HBC 2} Daepung 0923, whose color was pale yellow, was selected (see Example 2).

In another embodiment of the present invention, the color-making genetic region of the growing lineage plants including _{HBC 2} Daepung 0923 was identified and the genetic similarity was analyzed, and the color-related genetic region of _{HBC 2 Daepung 0722 through resequencing was 2.45.} It was found that Mb was inserted (see Example 3).

In another embodiment of the present invention, _{in order to analyze the agricultural traits of HBC 2} Daepung 0722, intrinsic characteristics, general characteristics, nutritional quality, and quantity were compared with the existing cultivar Daepung, and as a result, representatively, HBC ₂ Daepung 0743 It was confirmed that the color of silver was replaced with a pale yellow golden bean type compared to the Daepung bean, and the weight of the 100 grains was slightly increased, and the enteral length, the number of narrow numbers per individual, and the yield decreased somewhat (see Example 4).

_{The present inventors deposited the HBC 2} Daepung 0722 obtained through the above example to the Agricultural Genetic Resource Center of the National Academy of Agricultural Sciences on September 27, 2019, and received the accession number KACC 98065P.

In the present invention, the HBC ₂ Daepung0723 lineage plant has similar yield characteristics and no color control compared to Daepung, so it can be used for color improvement of soybean varieties for soybeans and mesophilic varieties.

The term “Hilum color” as used in the present invention is one of the characteristics of beans that affects the appearance quality and processing aptitude of the beans, and refers to the color of the eye area of the bean seeds. In the present invention, the colorless bean means yellowish color, more preferably pale yellow color.

_{The HBC 2} Daepung0723 lineage plant according to the present invention may have a weight of 23 to 26 g, preferably 24 to 25 g, and most preferably 24.6 g, but is not limited thereto.

A plant characterized in that it is a short-lived species of 57 to 62 cm, and the average narrow number is 22 to 26.

_{The HBC 2} Daepung0723 lineage plants according to the present invention have an average length of 57 to 62 cm and an average of 22 to 26, preferably an average length of 58 to 60 cm and an average of 23 to 25 It may be a dog, most preferably, the average length of the length is 59 cm and the average narrow number may be 24, but is not limited thereto.

As another aspect of the present invention, the present invention provides a _{method for breeding the HBC 2} Daepung 0743 lineage plants comprising the following steps.

_{(a) cultivating a hybrid (F 6} ) by artificial crossing using Daepung bean as a crossing model and golden bean as a crossing copy, and then selecting a line in which the target region corresponding to the color-making-related locus is minimally introduced;

_{(b) performing cross-breeding twice using the plant (BC 1} F ₂ ) obtained by sowing the selected lineage plant as a model, and using Daepung bean as a backup;

(c) selecting a plant having the highest similarity to Daepung from among the plants obtained after the overcrossing; And

(d) obtaining seeds from the selected plants and developing them to select a line whose color is yellow in _{BC 2} F _{2 generation.}

As used in the present invention, the term "bred parent" refers to parents used as mating materials in order to cultivate excellent descendants in breeding, and in the present invention, Daepung bean varieties grown by crossing of Baegunkong/Shinpaldalkong No.2 were used. , "Mating copy" means to be used as a mating material having a trait to be introduced, and in the present invention, a golden bean variety grown by crossing of Gwanggyo / Clark 63 / / Baekmokjangyeop was used.

In the step (a), the step of selecting a line in which the target region corresponding to the color-making-related locus has been introduced to the minimum may be performed through next-generation sequencing (NGS).

In the above step (c), only those confirmed to have been crossed are selected for the hybridization obtained after two times of crossbreeding, and individuals with high similarity to Dapoong are selected using 202 insertion/deletion (Indel) molecular markers, and then, It may further include the process of selecting a plant in which the second cross-breeding of the Daepung bean family has been confirmed in the individual.

In the step (c), selecting the plant having the highest similarity to Daepung is for the entire mutant area obtained by analyzing the single base variation (SNV) dense area using full-length genome information obtained through next-generation sequencing analysis (NGS). It can be done based on information.

Hereinafter, a preferred embodiment is presented to aid the understanding of the present invention. However, the following examples are provided for easier understanding of the present invention, and the contents of the present invention are not limited by the following examples.

[Example]

Example 1. Selection of mating parental copy

The inventors of the present invention used'Daepoong', a cultivar that is resistant to diseases and has high cultivation stability, and has excellent yield, but has a low preference for brown color, and selected 24 soybean cultivars in order to select the most genetically similar breed. Chromosomes were reanalyzed. To this end, next-generation sequencing (NGS) is performed on the 24 cultivars to obtain full-length genome information of each cultivar, and single nucleotide mutations based on the full-length genome information of the decoded cultivar. variant; SNV) clusters were analyzed to search for the entire variation block (VB) of 24 varieties. More specifically, single nucleotide variation (SNV) was detected while comparing the nucleotide sequence information of the cultivar to be analyzed with the standard genome in units of 10 kb. At this time, when the number of single base variants (SNVs) showing differences from the standard genome genome information was 4 or more per 10 kb, this was classified as a density variation block (dVB). When the adjacent mutation region is within a 90 kb interval, it is combined and expressed as one mutation region. In addition, the regions other than the mutation region were classified as a sparse variation block (sVB), and when the neighboring common regions were within a 30 kb interval, they were marked as the same region. By the above method, the mutation regions for each of the 24 varieties were analyzed, and as a result, as shown in FIG. 1, it could be divided into 6,147 mutation blocks.

Next, in order to use a cultivar that is genetically similar to the mating parent, Dapoong, but without color, as a mating copy, similarity between cultivars based on the mutation block obtained as a result of the analysis was analyzed. As a result, as shown in Figure 2, the bean varieties without color are Daewon (DW), Golden (HK), Jangproteinmok (JDBM), Jinpum 2 (JP2), Jangyeop (JY), Gwanggyo (KG), and Shinrok (SR). ) And Taekwang (TK) were identified as eight of them, of which Gold and Taekwang had the highest similarity to Daepung (DP). Accordingly, from the above two varieties, gold, which has excellent seed appearance characteristics, was selected as the mating copy.

Example 2. Process of fostering intermediate mothers

The inventors of the present invention have grown a middle model according to the process shown in Figure 3 to improve the color of the Daepung bean variety. More specifically, the cultivar selected in Example 1, gold, is a cultivar grown by crossing of Gwanggyo/Clark 63// Baekmokjangyeop, and Daepung is a cultivar grown by crossing of Baegunkkong/Shinpaldalkong No.2, and the National Institute of Food Science and Technology. The original species were used to cultivate the Golden/Dafeng recombinant (RIL) line. _{Among them, HD-487-F 6} strains with the least target region for color-making-related loci were selected, and female crossings were carried out using Daepung Kong as a backbone, and two of the six crosses were selected as a model. It was confirmed to have been mated. Next, by sowing the two confirmed matings (BC ₁ F _{1 ) and performing crossing on the BC 1} F ₂ plants, all 30 mating grains were obtained, of which 15 were finally mated. Confirmed. For the above 15 subjects, one of the 15 subjects with high similarity to'Daepung' was selected by background selection using 202 InDel molecular markers discovered by the present inventor through previous research. Seven plants whose matings were confirmed by secondary crossbreeding were secured. Subsequently, among the seven plants, 202 InDels were used to select plants having the highest similarity of 88% (98% including hetero) to'Daepoong', and three seeds were obtained therefrom. By developing this _{, 4 plants with BC 2} F _{2 were obtained, and HBC 2} Daepung 0923 with a pale yellow color was selected.

Example 3. Identification of colorimetric genetic region of breeding line and analysis of genetic similarity

_{In order to confirm the coloration genetic region of HBC 2} Daepung 0923, which is the breeding line obtained through Example 2 above, and to analyze its genetic similarity, the present inventors used the next-generation sequencing analysis to analyze Geum, Daepung, Line 1, and HBC ₂ Daepung. As a result of comparative analysis using Glycine_max_v2.0 (GCA_000004515.3) and 933.09 (Mb) as a standard genome (Williams 82), and performing a genome analysis on 0723 and 4 line, first, as shown in Table 1 below, gold (Average depth, 39.8X), Daepung (39.67), Line 1 (40.87), HBC ₂ Daepung 0723 (39.67) and Line 4 (43.16) were all deciphered to a level suitable for platform development.

Sample ID Total reads Clean reads (%) Total bases (bp) Clean bases (%) Mapped reads Average depth Gold 336104502 327980134
(97.58) 50415675300 48597133177 (96.39) 258577057
(96.74) 39.8 Great wind 334613914 328239672
(98.10) 50192087100 48653655301
(96.93) 257209324
(97.69) 39.67 System 1 336446804 329068812
(97.81) 50467020600 48743548801
(96.58) 265232887
(97.54) 40.87 HBC ₂ Daepung0723 336666594 329661444
(97.92) 50499989100 48863146199
(96.76) 257277045
(96.88) 39.61 Line 4 371279438 363836554
(98.00) 55691915700 53939580151
(96.85) 280167326
(96.96) 43.16

Further, as a result of comparing the similarity of the genome with Daepung, it was confirmed that the first line (86.57%), HBC ₂ Daepung 0743 (85.71%), and the fourth line (80.34%) as shown in FIG. In addition, as can be seen in Figure 5, the number of common SNPs in the distribution and number of SNPs of the four lines was similar, but it was confirmed that there are many unique SNPs of each line. In addition, in _{the case of HBC 2} Daepung0723, as shown in FIG. 6, it was verified through resequencing that 2.45 Mb was inserted into the color-related genetic region.

Example 4. Analysis of agricultural traits of breeding line

The present inventors compared various characteristics with the existing variety, Daepung, in order to analyze the agricultural traits of the breeding line obtained through Examples 1 to 3, that is, HBC _{2 Daepung 0722.}

First, the differences between the breeding system and the existing varieties according to the present invention are summarized and shown in Table 2 below. More specific results of comparative analysis by characteristics are as follows.

division Upbringing system Existing varieties Variety (line name) (HBC ₂ Daepung0723) Great wind Nurturing process HD487 ^*1 /2/Daefeng Bean Baekwoon Bean/Shin Paldal Bean No. 2 Color Buff Soak Color genetic region Replaced with golden bean type Daepung bean type ^*2 Out of 100 (g) 24.6 23.0 Yield (kg/10a) 410 459

4-1. Comparison of intrinsic characteristics

Intrinsic characteristics between the breeding line and the existing cultivar were compared, and the results are shown in Table 3 below. _{More specifically, it was confirmed that the peculiar characteristic of HBC 2} Daepung 0723, which is a breeding line, is a finite new growth type, a white flower color, an ovate leaf type, and an oval shape of a seed thread. Also, the seed coat color is yellow and the hair color is brown. In particular, _{it can be seen that the color of HBC 2} Daepung 0923 is yellow, and the Daepung bean is light brown, showing a distinct difference.

Variety name New type Flower color Leaf type Hair color Pod color Bell color Bell shape Color Degranulation HBC ₂ Daepung0723 Finite White Ovate grey Brown yellow rectangle yellow I Daepung Bean Finite White Ovate grey Brown yellow rectangle Light brown I

4-2. General characteristics comparison

As a result of comparing the general characteristics between the breeding line and the existing cultivars, as shown in Table 4 below, _{the flowering and maturation period of HBC 2} Daepung 0923 is a middle-aged species similar to the comparison variety Daepung Bean, and the length of the plant is 59 cm, compared to 63 cm of Daepung Bean. It can be seen that it is a 4 cm small short-lived bell that is resistant to uniforms. The number of nymphs per individual was 24, which was slightly decreased compared to Daepungkong, and the weight of 100 grains was 24.6 g, which was confirmed to be a medium allele which slightly increased compared to 23.0 g of Daepungkong. Figure 7 shows the difference between the breeding system and some varieties, including the existing varieties (g), among the hard length and 100 grains. The results of this comparison of characteristics are summarized on the basis of the results analyzed by sowing on June 10 in Jinbuyeon, Pyeongchang-gun, Gangwon-do, 2018.

Variety name Flowering period (Monday) Maturity (Monday) Length (cm) Uniform Main light saving water (pcs)) Narrow number (pcs) Out of 100 (g) HBC ₂ Daepung0723 7.26 10.19 59 River 13 24 24.6 Daepung Bean 7.26 10.17 63 River 16 34 23.0

4-3. Quality comparison

Next, the nutrient content of the breeding system according to the present invention and the existing variety, Daepungwa, was analyzed and compared. As a result, as shown in Tables 5 and 6, it was confirmed that _{the protein and lipid content of HBC 2} Daepung 0923 was similar to that of Daepung bean, and the isoflavone content was somewhat lower than that of Daepung Bean. On the other hand, vitamins B ₁ , B ₂ and niacin were higher than that of Daepung bean, and in the case of calcium and phosphorus among inorganic ingredients, HBC ₂ Daepung 0923 had the advantage of containing more than Daepung bean.

Variety name protein
(g/100g) Fat
(g/100g) Ash
(g/100g) Isoflavones
(㎍/g) Vitamin (mg/100g) Vitamin B _One Vitamin B ₂ Niacin HBC ₂ Daepung0723 39.00 14.23 5.35 5,376 1.05 0.32 1.89 Daepung Bean 39.74 15.73 4.68 5,516 0.81 0.23 1.34

Variety name Inorganic ingredients (mg/100g) Amino acid composition (%) calcium sign iron potassium Methionine Cysteine Lysine HBC ₂ Daepung0723 176.79 735.66 6.04 1802.63 0.48 0.55 2.43 Daepung Bean 156.22 692.10 7.31 1909.55 0.47 0.58 2.48

4-4. Quantitative comparison

Finally, the yield was compared between the breeding line and the existing variety, and the results are shown in Table 7 and FIG. 7 below. As a result of the analysis, it was confirmed that _{the yield of HBC 2} Daepung 0722 was 410 kg/10a when cultivated vinyl mulching in Jinbu in 2018, which was 11% lower than that of Daepung Bean.

Variety name Quantity (kg/10a) Indices(%) HBC ₂ Daepung0723 410 89 Daepung Bean 459 100

The above-described description of the present invention is for illustrative purposes only, and those of ordinary skill in the art to which the present invention pertains can understand that it is possible to easily transform it into other specific forms without changing the technical spirit or essential features of the present invention. There will be. Therefore, it should be understood that the embodiments described above are illustrative and non-limiting in all respects.

National Institute of Agricultural Sciences Agricultural Genetic Resource Center KACC98065P 20190927

Claims (11)

_{HBC 2} Daepung0723 lineage plant for color improvement of soybean varieties deposited with the registration number KACC 98065P at the Agricultural Genetic Resource Center of the National Academy of Agricultural Sciences.
The method of claim 1,
The bean is characterized in that for long and mesophilic species, plants.
The method of claim 1,
Plant, characterized in that the color is yellow.
The method of claim 1,
The HBC ₂ Daepung0723 lineage plant is characterized in that 23 to 26 g of the white grain, plant.
The method of claim 1,
The HBC ₂ Daepung0723 lineage plants are short-lived species with an average length of 57 to 62 cm, and an average narrow number of 22 to 26, a plant.
The seeds according to any one of claims 1 to 5 of the HBC ₂ Daepung0723 line plant.
The progeny of a seed _{according to the HBC 2} Daepung0723 line plant according to any one of claims 1 to 5.
The breeding method of the _{HBC 2} Daepung0723 line plant of claim 1, comprising the following steps:
_{(a) cultivating a hybrid (F 6} ) by artificial crossing using Daepung bean as a crossing model and golden bean as a crossing copy, and then selecting a line in which the target region corresponding to the color-making-related locus is minimally introduced;
_{(b) performing cross-breeding twice using the plant (BC 1} F ₂ ) obtained by sowing the selected lineage plant as a model, and using Daepung bean as a backup;
(c) selecting a plant having the highest similarity to Daepung from among the plants obtained after the overcrossing; And
(d) obtaining seeds from the selected plants and developing them to select a line whose color is yellow in _{BC 2} F _{2 generation.}
The method of claim 8,
In the step (a), the step of selecting the lineage in which the target region corresponding to the color-removing-related locus has been introduced to the minimum is performed through next-generation sequencing (NGS), a breeding method.
The method of claim 8,
In the step (c), selecting the plant having the highest similarity to Daepung is for the entire mutant area obtained by analyzing the single base variation (SNV) dense area using the full-length genome information obtained through next-generation sequencing analysis (NGS). Characterized in that based on information, breeding method.
The method of claim 8,
The HBC ₂ Daepung0723 lineage plant is characterized in that 2.45 Mb is inserted into the color-related genetic region, breeding method.

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