KR20210056013A - Pharmaceutical composition for preventing or treating retinal disease comprising extract of black rice - Google Patents

Abstract

The present invention provides a pharmaceutical composition for preventing or treating retinal disease containing an extract or a fraction thereof of black rice, a food composition for preventing or alleviating retinal disease containing the extract or the fraction thereof, a feed additive composition for preventing or alleviating retinal disease containing the extract or the fraction thereof, and a method for preventing or treating retinal diseases using the pharmaceutical composition. The composition containing the extract of black rice or the fraction thereof provided in the present invention has no side effects and can prevent or alleviate retinal diseases, thereby being able to be widely used in the development of formulations for preventing or treating various types of ophthalmic diseases, including retinal diseases.

Description

Pharmaceutical composition for preventing or treating retinal disease comprising extract of black rice}

The present invention relates to a pharmaceutical composition for preventing or treating retinal diseases comprising a black rice extract, and more specifically, the present invention, a pharmaceutical composition for preventing or treating retinal diseases comprising an extract of black rice or a fraction thereof, the extract or its It relates to a food composition for preventing or improving retinal disease comprising a fraction, a composition for preventing or improving retinal disease including the extract or a fraction thereof, and a method for preventing or treating retinal disease using the pharmaceutical composition.

The eyes of animals, including humans, play an important role in transmitting information through recognition of surrounding information. In other words, it performs various functions to induce actions appropriate to the situation by constantly receiving accurate images of the surrounding environment through the eyes and processing the information received from the brain. Like other organs in the body, various eye diseases occur in the eye.

These ophthalmic diseases are mainly caused by impaired functions of the cornea or retina. Since the cornea can be easily damaged by the external environment, it is known that the majority of patients with ophthalmic diseases are due to corneal damage. Such corneal damage can be recovered by drug or surgical treatment, and a representative example of such surgical treatment is refractive corneal surgery using an excimer laser.

In contrast, the retina is hardly affected by the external environment, and it is known that retinal diseases are caused by physical or genetic factors. A typical example of such a physical factor is aging, and it is known that when aging progresses, the likelihood of developing macular damage caused by a decrease in the function of the macula present in the retina increases. Unlike corneal diseases, since the retinal disease is difficult to treat by an ectopic surgical treatment, drug therapy, gene therapy, or stem cell therapy, which has been actively developed in recent years, are generally used.

For example, Korean Patent Laid-Open No. 2017-0028132 discloses a pharmaceutical composition for preventing and treating retinal diseases including extracts derived from small groups, and Korean Patent Publication No. 2017-0049775 discloses a retinal containing miR-410 inhibitor. A composition for inducing pigment epithelial differentiation is disclosed, and Korean Patent Publication No. 2017-0055012 discloses self-assembled and condensed high molecular weight siRNA core particles and a large amount inside the retina by sequentially coating the outside with cationic polymer and hyaluronic acid. Particles that efficiently deliver siRNA of and a method for preparing the same are disclosed. However, these therapeutic agents are known to have an effect of regenerating or restoring damaged retinal tissue after the retinal tissue is damaged due to the onset of retinal disease, but not having an effect of suppressing damage to the retinal tissue.

Under this background, the present inventors have made diligent research efforts to develop a formulation capable of effectively preventing or treating retinal diseases, and as a result, it has been confirmed that the recently developed extract of black rice has an effect of suppressing damage to retinal pigment epithelial cells, The present invention has been completed.

One object of the present invention is to provide a pharmaceutical composition for preventing or treating retinal diseases comprising an extract of black rice or a fraction thereof.

Another object of the present invention is to provide a method for preventing or treating retinal diseases by using the pharmaceutical composition.

Another object of the present invention is to provide a food composition for preventing or improving retinal diseases comprising an extract of black rice or a fraction thereof.

Another object of the present invention is to provide a composition for adding feed for preventing or improving retinal diseases comprising an extract of black rice or a fraction thereof.

As an embodiment of achieving the above object, the present invention provides a pharmaceutical composition for preventing or treating retinal diseases comprising an extract of black rice (KACC 98047P) or a fraction thereof.

The term "heukmi rice (KACC 98047P)" of the present invention is a new variety of heukmi rice selected by artificial crossing Hana-reum rice and early heuk-chal, and the watering period from August 20 to September 5; Soy sauce of 70 to 85 cm; A head of 15 to 20 cm; 15 to 20 sorghum; 75 to 95 allowances; Brown rice quantity of 450 to 550 kg/10a; Protein content of 4 to 6%; And 15 to 25% amylose content, and a patent was deposited at the Agricultural Genetic Resource Center of the National Academy of Agricultural Sciences located in Seodun-dong, Gwonseon-gu, Suwon-si, Gyeonggi-do on April 18, 2016 under the deposit number KACC 98047P. It can be easily obtained through an institution or the like.

The term "extract" of the present invention is an extract obtained by extracting the fruits, stems, leaves, roots or pulverized products of the black rice, the dilution or concentrate of the extract, the dried product obtained by drying the extract, and the adjustment of the extract. It includes extracts of all formulations that can be formed using the extract itself and the extract, such as preparations, purified products, or mixtures thereof.

In the present invention, the method of extracting the black rice is not particularly limited as long as an extract exhibiting an effect of preventing or treating retinal diseases can be obtained, and can be extracted according to a method commonly used in the art. have. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination of two or more methods.

In the present invention, the type of extraction solvent used to extract the black rice is not particularly limited as long as an extract exhibiting an effect of preventing or treating retinal diseases can be obtained, and any solvent known in the art may be used. I can. As an example, as the extraction solvent, water or alcohol may be used alone or in combination of one or more, and as another example, water or an alcohol having 1 to 4 carbon atoms may be used alone or as the extraction solvent. It may be mixed and used, and as another example, water may be used as the extraction solvent.

The term "fraction" in the present invention means a result obtained by performing fractionation in order to separate a specific component or a specific group of components from a mixture containing several different constituents.

In the present invention, the fraction may be a result of fractionation of the black rice extract by various methods such as a solvent fractionation method, an ultrafiltration fractionation method, and a chromatography fractionation method.

The method of obtaining the fraction is also not particularly limited as long as it can obtain a fraction exhibiting an effect of preventing or treating retinal diseases, and may be performed according to a method commonly used in the art. As an example, a method of obtaining a fraction from a hot water extract of black rice using a solvent fractionation method may be used.

The type of solvent fraction used to obtain the fraction in the present invention is not particularly limited as long as a fraction exhibiting an effect of preventing or treating retinal diseases can be obtained, and it is performed according to a method commonly used in the art. Can be. As an example, the fractionation solvent may include a polar solvent such as water and alcohol; Non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane may be used alone or in combination of one or more.

The term "retinal disease" of the present invention refers to a disease caused by damage to the retina due to physical or genetic factors.

In the present invention, the retinal disease is not particularly limited as long as it can be prevented or treated by an extract of black rice or a fraction thereof, but as an example, it may be a retinal disease caused by damage to retinal pigment epithelial cells, and other examples As an example, it may be retinal pigment epithelial cell damage caused by retinal pigment epithelial cell damage, senile macular degeneration, NCMD (North Carolina Macular), and the like.

The term "prevention" of the present invention refers to any action of inhibiting or delaying the onset of retinal disease by administration of a pharmaceutical composition comprising the extract of black rice or a fraction thereof of the present invention.

The term "treatment" of the present invention refers to any action in which symptoms of retinal disease are improved or beneficially changed by administration of the pharmaceutical composition.

The pharmaceutical composition of the present invention may contain 0.0001 to 50% by weight of a black rice extract or a fraction thereof based on the weight of the total composition, and specifically 0.01% to 10% by weight, but is not limited thereto.

The pharmaceutical composition of the present invention may further include a pharmaceutically acceptable carrier, excipient, or diluent commonly used in the manufacture of pharmaceutical compositions, and the carrier may include a non-naturally occurring carrier. I can.

The term "pharmaceutically acceptable" of the present invention means exhibiting properties that are not toxic to cells or humans exposed to the composition.

Specifically, the pharmaceutical composition is formulated in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, and sterile injectable solutions according to a conventional method. I can.

In the present invention, the carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, Calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations include at least one excipient, such as starch, calcium carbonate, sucrose, or lactose. It is prepared by mixing (lactose) and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include water and liquid paraffin, which are simple diluents commonly used for suspensions, liquid solutions, emulsions, syrups, etc., and various excipients such as wetting agents, sweetening agents, fragrances, and preservatives. have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations, and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogelatin, and the like may be used.

In another aspect, the present invention provides a method for preventing or treating retinal diseases comprising administering the pharmaceutical composition to an individual other than a human who has or is likely to develop retinal disease.

The term "administration" of the present invention means introducing a given substance to a subject in an appropriate manner.

The term "individual" of the present invention means all animals such as rats, mice, livestock, including humans who have or may develop retinal disease. As a specific example, it may be a mammal including a human.

The method for preventing or treating retinal diseases of the present invention specifically includes the step of administering a pharmaceutical composition for preventing or treating retinal diseases including an extract of black rice or a fraction thereof to an individual other than humans in a pharmaceutically effective amount. I can.

As used herein, the term "pharmaceutically effective amount" refers to an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment and does not cause side effects, and the effective dose level is the gender of the patient. , Age, weight, health condition, type of disease, severity, activity of the drug, sensitivity to the drug, method of administration, time of administration, route of administration, and rate of excretion, duration of treatment, factors including drugs used in combination or simultaneously, and others It can be readily determined by a person skilled in the art according to factors well known in the medical field.

Specifically, the composition of the present invention may be administered in an amount of 0.0001 to 100 mg/kg body weight per day based on solid content, and more specifically at 0.001 to 100 mg/kg body weight per day. Administration may be administered once a day at the above recommended dosage, or may be divided several times.

In the method for preventing or treating retinal diseases of the present invention, the route of administration and the method of administration for administering the composition are not particularly limited, and any route of administration and administration as long as the composition containing the composition can reach the target site. It can be according to the mode of administration. Specifically, the composition may be administered through various routes, either oral or parenteral, and non-limiting examples of the route of administration include oral, rectal, topical, intravenous, intraperitoneal, intramuscular, intraarterial, transdermal, non- And those administered through intralateral or inhalation.

In another aspect, the present invention provides a food composition for preventing or improving retinal diseases comprising an extract of black rice or a fraction thereof.

Since the black rice can be consumed for food, its extracts or fractions can also be prepared and consumed in the form of foods capable of preventing or improving immune tissue or organ damage while having common sense.

The term "improvement" of the present invention refers to all actions of at least reducing the severity of a parameter related to the condition to be treated, for example, symptoms, by administration of a composition comprising an extract of black rice or a fraction thereof of the present invention.

The term "food" in the present invention means meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages , Vitamin complexes, health functional foods, and health foods, and all foods in the usual sense are included.

Since the food composition of the present invention can be consumed on a daily basis, a high retinal disease improvement effect can be expected, and thus it can be very usefully used for health promotion purposes.

The health function (functional food) is the same term as food for special health use (FoSHU). In addition to supplying nutrition, functional food is a medicine that is processed so that the bioregulatory function is effectively displayed, and has high medical effects. Means food. Here, the term'function (sex)' means to control nutrients for the structure and function of the human body or to obtain useful effects for health purposes such as physiological actions. The food product of the present invention can be prepared by a method commonly used in the art, and during the production, raw materials and ingredients commonly added in the art may be added to prepare the food product. In addition, the formulation of the food may be prepared without limitation as long as it is a formulation recognized as a food. The food composition of the present invention can be prepared in various forms of formulation, and unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long time using food as a raw material, and is excellent in portability. The food of the present invention can be ingested as an adjuvant for enhancing the effect of improving retinal diseases.

The health food refers to a food having an active health maintenance or promotion effect compared to a general food, and a health supplement food refers to a food for the purpose of health supplementation. In some cases, the terms health functional food, health food, and health supplement are used interchangeably.

Specifically, the health functional food is a food prepared by adding the compound of the present invention to food materials such as beverages, teas, spices, chewing gum, confectionery, or encapsulating, powdering, suspension, etc. It means bringing about an effect, but unlike general drugs, it has the advantage of having no side effects that may occur when taking the drug for a long time by using food as a raw material.

The food composition may further include a physiologically acceptable carrier, and the kind of the carrier is not particularly limited, and any carrier commonly used in the art may be used.

In addition, the food composition may include additional ingredients that are commonly used in food compositions to improve smell, taste, vision, and the like. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid, and the like may be included. In addition, minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu), and chromium (Cr); And amino acids such as lysine, tryptophan, cysteine, and valine.

In addition, the food composition includes preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), disinfectants (bleaching and highly bleaching, sodium hypochlorite, etc.), antioxidants (butylhydroxyanisol (BHA), butylhydride, etc.) Oxytoleuene (BHT), etc.), coloring agent (tar color, etc.), coloring agent (sodium nitrite, sodium nitrite, etc.), bleach (sodium sulfite), seasoning (MSG sodium glutamate, etc.), sweetener (dulsin, cyclamate, saccharin, etc.) , Sodium, etc.), flavoring (vanillin, lactones, etc.), expanding agents (alum, D-potassium hydrogen stannate, etc.), reinforcing agents, emulsifying agents, thickening agents (smelting agents), coating agents, gum base agents, foam inhibitors, solvents, improving agents, etc. It may contain food additives. The additive may be selected according to the type of food and used in an appropriate amount.

As an example of the food composition of the present invention, it may be used as a health drink composition, and in this case, it may contain various flavoring agents or natural carbohydrates, etc. as an additional component, such as a conventional beverage. The natural carbohydrates described above include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Polysaccharides such as dextrin and cyclodextrin; It may be a sugar alcohol such as xylitol, sorbitol, and erythritol. Sweeteners include natural sweeteners such as taumatin and stevia extract; Synthetic sweeteners such as saccharin and aspartame can be used. The ratio of the natural carbohydrate may be generally about 0.01 to 0.04 g, specifically about 0.02 to 0.03 g per 100 mL of the health beverage composition of the present invention.

In addition to the above, health drink compositions include various nutrients, vitamins, electrolytes, flavoring agents, colorants, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, It may contain alcohol or a carbonation agent. In addition, it may contain flesh for the manufacture of natural fruit juice, fruit juice beverage, or vegetable beverage. These ingredients may be used independently or in combination. Although the ratio of these additives is not very important, it is generally selected from 0.01 to 0.1 parts by weight per 100 parts by weight of the health beverage composition of the present invention.

In another aspect, the present invention provides a composition for adding feed for preventing or improving retinal diseases, including an extract of black rice or a fraction thereof.

Since the extract of black rice or its fractions can suppress damage to retinal pigment epithelial cells, the composition of a feed additive composition that can be added to animal breeding feeds that can prevent or improve retinal diseases caused in livestock, etc. It is manufactured in a form, and can be fed to animals by preparing a feed for animal breeding comprising the composition for feed addition.

The composition for feed addition of the present invention may further include excipients and diluents that can be used in the manufacture of feed. The excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, Polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, stevia extract and natural material extract.

In addition, the composition for feed addition of the present invention includes not only the extract of the black rice or its fractions, but also nucleotides, enzymes, and trace components necessary to completely supply essential nutrients necessary for improving the productivity or health of livestock, as well as the growth of livestock. It may further include a microorganism that assists. For example, as nucleotides, 5'-adenylic acid, xanthic acid, ribonucleic acid, deoxyribonucleic acid, and the like can be used alone or in combination; Enzymes include arabinase, arabinofuranosidase, galactanase, pectinase, maltase, invertase, and lipase. ) And the like may be used alone or in combination; As trace components, amino acids, calcium, phosphoric acid, organic acids, etc. may be used alone or in combination; Microorganisms include Leukonostoc sp. strain, Weisella sp. strain, Enterococcus sp. strain, Streptomyces sp. strain, and Nocardia sp. Nocardia sp.) strains and the like may be used alone or in combination.

In another aspect, the present invention provides a feed for livestock comprising the composition for adding feed and exhibiting a preventive or improving effect of retinal diseases.

As described above, when the extract of black rice or a fraction thereof provided in the present invention is fed to animals, it can prevent or improve retinal disease without special toxicity. It can have the effect of improving the health of the body.

Livestock capable of feeding the feed for livestock provided by the present invention is not particularly limited as long as it exhibits an effect of preventing or improving retinal diseases by an extract of black rice or a fraction thereof, but as an example, chicken, duck, goose , Cows, goats, sheep, horses, dogs, pigs, etc.

The term "raw material feed" of the present invention means a feed to which the feed additive composition is added.

In the present invention, the raw material feed is not particularly limited as long as the feed additive composition is added to show an effect of preventing or improving retinal diseases of livestock, but as an example, rice, fermented soybean meal, soybean, Italian grass, Sudangrass, rye, rice straw, corn, wheat bark, rice bran, sugar bark, etc. may be used alone or in combination to be a compounded feed containing as main ingredients, and these main ingredients are included in the appropriate range generally used in raw material feed for livestock. I can.

The term "mixed feed" of the present invention refers to a feed made by blending so that nutrients suitable for the purpose of raising livestock are evenly supplied, and the blended feed is nutrients such as energy, protein, lipid, vitamins, minerals, etc. required by livestock. Includes. When making mixed feed, from a nutritional point of view, an appropriate amount of solids is indispensable in order to enhance the appetite of livestock and to promote the normal functioning of the digestive system, and to fully demonstrate the production activities of livestock. Proper amount of protein must be added, calories must be included to maintain the body temperature and production activities of livestock normally, and other minerals such as calcium or phosphorus and vitamins must be sufficiently contained.

Meanwhile, the content of the feed additive composition included in the feed is not particularly limited thereto, but as an example, it may be 0.01 to 10% (w/w) based on the final weight of the feed, and as another example, 0.1 to 5 It can be% (w/w), and as another example, it can be 1% (w/w).

In addition, the feed contains plasma proteins, fish meal, whey, complex dairy products, soybean oil, amino acid premix, acidulant premix, red ginseng meal, salt, vitamin-mineral mixture, etc. It may be further included as or mixed.

Since the composition containing the extract of black rice or its fraction provided by the present invention can prevent or improve retinal diseases without side effects, it is widely used in the development of preparations for preventing or treating various types of ophthalmic diseases including retinal diseases. It will be able to be utilized.

1 is a photomicrograph showing a proliferation result over time after culturing retinal pigment epithelial cells (ARPE-19 cells).
Figure 2a is a graph showing the change in the damage level of the retinal pigment epithelial cells (ARPE-19 cells) according to the treatment of the general black rice extract.
Figure 2b is a graph showing the change in the damage level of the retinal pigment epithelial cells (ARPE-19 cells) according to the treatment of the extract of the black rice of the present invention.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example One: Black rice Preparation of extract

First, an extraction solvent in which 0.1% acetic acid was added to 20 to 80% alcohol was obtained.

The extraction solvent was added to brown rice of black rice (KACC 98047P), followed by extraction with stirring at room temperature for 24 or 48 hours to obtain respective extracts.

Each of the obtained extracts was filtered to obtain a liquid component, the obtained liquid component was concentrated, and then freeze-dried to obtain a powdery extract, and the content of the obtained powdery extract was quantitatively analyzed (Table 1).

Quantitative analysis result of black rice extract (extract amount (g)/sample 100g) Extraction solvent 24 hours, room temperature 48 hours, room temperature 100% water (0.1% acetic acid) 4.2±0.4 4.3±0.2 20% alcohol (0.1% acetic acid) 6.2±0.8 6.4±0.4 50% alcohol (0.1% acetic acid) 10.5±0.5 10.8±0.4 80% alcohol (0.1% acetic acid) 11.4±0.3 12.8±0.7

As shown in Table 1, it was confirmed that the extract having the best content can be obtained when extracted at room temperature for 48 hours using an extraction solvent in which 0.1% acetic acid was added to 80% alcohol.

Example 2: Retinal pigment epithelial cells Damaging Black rice ( KACC 98047P) Effect analysis of extract

Example 2-1: Retinal pigment epithelial cells culture

In order to confirm the safety of the black rice extract obtained in Example 1, retinal pigment epithelial cells (ARPE-19) were cultured.

Specifically, maniac pigment epithelial cells were counted and dispensed into a 96-well plate at 2×10 ² cells/well, and black rice extract at concentrations of 10, 50 and 100 μg/mL were treated with the cells and cultured for 48 hours (Fig. One).

1 is a photomicrograph showing the results of proliferation over time after culturing retinal pigment epithelial cells (ARPE-19 cells).

Example 2-2: Black rice ( KACC 98047P) Effect of extract

First, according to the results of Example 1, extracts of black rice (KACC 98047P) brown rice and general black rice brown rice (control) were obtained, respectively, using an extraction solvent in which 0.1% acetic acid was added to 80% alcohol.

(General used as the control Black rice Please tell me the breed)

Next, the ARPE-19 cells were damaged by adding A2E (Pyridinum Bisretinoid) and blue light (BL) to the ARPE-19 cells cultured in Example 2-1, and each of the obtained extracts was applied to the damaged ARPE-19 cells. Was added at various concentrations (10 to 100 μg/ml) and cultured, and then the cell viability of the cultured ARPE-19 cells was compared (FIGS. 2A and 2B ).

Figure 2a is a graph showing the change in the damage level of the retinal pigment epithelial cells (ARPE-19 cells) according to the treatment of the general black rice extract, Figure 3b is the retinal pigment epithelium according to the treatment of the extract of the black rice of the present invention It is a graph showing the change in the damage level of cells (ARPE-19 cells).

As shown in Fig. 2a, when the general black rice extract was treated, it was confirmed that the survival rate of damaged ARPE-19 cells did not change even when treated at a high concentration (100 µg/ml).

On the other hand, as shown in FIG. 2B, when the extract of black rice (KACC 98047P) was treated, the survival rate of damaged ARPE-19 cells was increased even when the extract was treated with a low concentration (25 μg/ml). It was confirmed that as the treatment concentration increased, the survival rate of damaged ARPE-19 cells increased proportionally.

Therefore, it was found that the extract of Black Rice (KACC 98047P) showed an effect of suppressing the damage of retinal pigment epithelial cells caused by external factors in a concentration-dependent manner.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features thereof. In this regard, the embodiments described above are illustrative in all respects and should be understood as non-limiting. The scope of the present invention should be construed as including the meaning and scope of the claims to be described later rather than the above detailed description, and all changes or modifications derived from the equivalent concepts within the scope of the present invention.

Korea Agricultural Microbial Resource Center KACC98047P 20160418

Claims (11)

A pharmaceutical composition for preventing or treating retinal diseases comprising an extract of black rice (KACC 98047P) or a fraction thereof.
The method of claim 1,
The extract is obtained by extracting black rice (KACC 98047P) with at least one solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof.
The method of claim 1,
The fraction is obtained by fractionating the extract with at least one solvent selected from the group consisting of a polar solvent, a non-polar solvent, and a mixed solvent thereof.
The method of claim 3,
The polar solvent is water or alcohol.
The method of claim 3,
The non-polar solvent is one or more solvents selected from the group consisting of hexane, ethyl acetate, chloroform, dichloromethane, and mixed solvents thereof.
The method of claim 1,
The retinal disease is a disease caused by damage to retinal pigment epithelial cells, pharmaceutical composition.
The method of claim 6,
The disease caused by the retinal pigment epithelial cell damage is one or more diseases selected from the group consisting of retinal pigmentation, senile macular degeneration, NCMD (North Carolina Macular), and combinations thereof.
The method of claim 1,
The composition further comprises a pharmaceutically acceptable carrier, excipient, or diluent.
A method for preventing or treating retinal diseases, comprising administering the composition of any one of claims 1 to 8 to an individual other than a human having or likely to develop retinal disease.
Black rice (KACC 98047P) extract or a food composition for improving retinal diseases comprising a fraction thereof.
Black rice (KACC 98047P) extract or a feed additive composition for improving retinal diseases comprising a fraction thereof.

Images