KR20210042664A - Method for opuntia humifusa extract containing antioxidant carbon nanoparticles and reduced viscosity using microwave - Google Patents

Abstract

The purpose of the present invention is to provide a method for producing an Opuntia humifusa extract, wherein viscosity-related problems of Opuntia humifusa can be resolved. Also, the purpose of the present invention is to provide a cosmetic composition or a food composition comprising an extract of Opuntia humifusa as an active ingredient using a method for producing an extract with reduced cytotoxicity of Opuntia humifusa. The method for producing an Opuntia humifusa extract containing antioxidant carbon nanoparticles using microwaves, wherein the method comprises the steps of: (a) pulverizing Opuntia humifusa; (b) producing an Opuntia humifusa solid by irradiating microwaves to the pulverized Opuntia humifusa; and (c) producing an Opuntia humifusa extract containing antioxidant carbon nanoparticles by adding distilled water to the Opuntia humifusa solid.

Description

A method of manufacturing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles and reduced viscosity using microwaves {METHOD FOR OPUNTIA HUMIFUSA EXTRACT CONTAINING ANTIOXIDANT CARBON NANOPARTICLES AND REDUCED VISCOSITY USING MICROWAVE}

The present invention relates to a method for producing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles using microwave, and more particularly, by a simple process of pulverizing the original Cheonnyeoncho and microwave treatment, processing and commercialization by lowering the viscosity of Cheonnyeoncho It relates to a method for preparing an extract to facilitate.

Opuntia humifusa (Opuntia humifusa) is a Korean native cactus that grows naturally in Chungnam and other places. Opuntia ficus-indica var saboten, generally known as opuntia cactus, grows to 1~2 m in size and has many long and thick thorns, whereas cheonnyeoncho is downy thorns. Take it and grow it to a size of about 30 cm.

Baengnyeoncho grown in Jeju Island is native to the tropical region and is difficult to cultivate in the open field because it is weak at low temperatures, whereas the Korean native cactus, Cheonnyeoncho, survives in the open field even in the dormant winter and has strong characteristics against pests and diseases.

There has not been much research on the activity of Cheonnyeoncho compared to the naturalized plant, Baengnyeoncho, but recently, the antioxidant activity of the stem extract of Cheonnyeoncho by hot water and the effect of preventing liver damage from carbon tetrachloride were reported. There has been a case where the acetate fraction has been reported to exhibit an antioxidant activity similar to that of α-tocopherol.

However, while Baengnyeoncho is actively used as food such as chocolate, Cheonnyeoncho is hardly processed and commercialized as foods and cosmetics despite the aforementioned physiological activity, which is due to the viscosity of pectin, the main ingredient of Cheonnyeoncho.

Up to now, attempts to remove the viscosity of Cheonnyeoncho have been suggested by radiation extraction, ultra-high pressure extraction, filtration, enzymatic hydrolysis, fermentation, and freezing and powdering of Cheonnyeoncho. Methods such as filtration or powdering have a problem that viscosity is not effectively removed, and enzymatic hydrolysis is cumbersome and takes a lot of time and cost. In addition, the radiation extraction method has a problem that may cause emotional antagonism due to radiation irradiation, and the ultra-high pressure extraction method requires a lot of cost and requires a separate mechanical device.

In addition, in the powdering method after freezing, when water is added to the powder and kneaded, the adhesive strength is strong, so that it sticks to the molding machine, and the granules stick to each other, making it difficult to formulate, so there is a problem that it is difficult to process and commercialize the millennial plant.

An object of the present invention is to provide a method for preparing an extract of Cheonnyeoncho that can solve the viscosity problem of Cheonnyeoncho.

In addition, it is an object of the present invention to provide a cosmetic composition and a food composition comprising an extract extracted using a manufacturing method with reduced cytotoxicity of Cheonnyeoncho.

In order to achieve the above object, the present invention comprises the steps of: (a) pulverizing Cheonnyeoncho; (b) irradiating microwaves on the pulverized cheonnyeoncho to prepare a cheonnyeoncho solid; And (c) adding distilled water to the solid material of Cheonnyeoncho to prepare a Cheonnyeoncho extract containing antioxidant carbon nanoparticles. It provides a manufacturing method of Cheonnyeoncho using a microwave comprising a.

In the step (a), leaves, fruits, and roots of Cheonnyeoncho may be used.

In step (b), the microwave irradiation may be performed at a temperature of 80 to 120°C.

In step (b), the microwave irradiation may be performed for 3 to 15 minutes.

In step (b), the microwave may be irradiated with 300 to 800 W.

The Cheonnyeoncho extract may have a viscosity of 1.0 to 3.0 mPa.S.

In addition, in order to achieve the above object, the present invention comprises the steps of: (a) pulverizing Cheonnyeoncho; (b) irradiating microwaves on the pulverized cheonnyeoncho to prepare a cheonnyeoncho solid; And (c) adding distilled water to the solid material of Cheonnyeoncho to provide a cosmetic composition comprising the Cheonnyeoncho extract prepared by the step of preparing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles as an active ingredient.

In addition, in order to achieve the above object, the present invention comprises the steps of: (a) pulverizing Cheonnyeoncho; (b) irradiating microwaves on the pulverized cheonnyeoncho to prepare a cheonnyeoncho solid; And (c) adding distilled water to the solid material of Cheonnyeoncho to provide a food composition comprising the Cheonnyeoncho extract prepared by the step of preparing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles as an active ingredient.

According to the method for preparing an extract of Cheonnyeoncho according to the present invention, it is possible to reduce the degree of cytotoxicity of Cheonnyeoncho while sufficiently lowering the viscosity of Cheonnyeoncho through a simple process.

According to the method for preparing a cheonnyeoncho extract according to the present invention, there is an advantage in that cheonnyeoncho, which has antioxidant and skin inflammation improvement effects, can be easily processed into cosmetics or foods and commercialized.

1 is a photograph showing the extract of Cheonnyeoncho according to Comparative Example 1 and Examples 1 to 4.
2 is a photograph comparing the viscosity of Cheonnyeoncho extracts according to Comparative Example 1 and Examples 1 to 4.
3 is a photograph showing the result of irradiating ultraviolet rays on the extract of Cheonnyeoncho according to Example 2.
4 is a graph showing the results of a cytotoxicity experiment when the Cheonnyeoncho extract according to Comparative Examples 1 and 2 was treated on HaCaT cells for 24 hours.
5 is a graph showing the results of a cytotoxicity experiment when the Cheonnyeoncho extract according to Comparative Examples 1 and 2 was treated on HaCaT cells for 48 hours.
6 is a graph comparing the results of apoptosis (apoptosis) when the Cheonnyeoncho extract according to Comparative Examples 1 and 2 was treated on HaCaT cells for 24 hours.
7 is a graph comparing the results of apoptosis (apoptosis) when the Cheonnyeoncho extract according to Comparative Examples 1 and 2 was treated on HaCaT cells for 48 hours.

In the following description, it should be noted that only parts necessary for understanding the embodiments of the present invention will be described, and descriptions of other parts will be omitted without distracting the gist of the present invention.

The terms or words used in the present specification and claims described below should not be construed as being limited to a conventional or dictionary meaning, and the inventor is appropriate as a concept of terms in order to describe his own invention in the best way. It should be interpreted as a meaning and concept consistent with the technical idea of the present invention on the basis of the principle that it can be defined. Therefore, the embodiments described in the present specification and the configurations shown in the drawings are only preferred embodiments of the present invention, and do not represent all the technical spirit of the present invention, and various equivalents that can replace them at the time of application It should be understood that there may be variations and variations.

The present invention comprises the steps of: (a) pulverizing Cheonnyeoncho; (b) irradiating microwaves on the pulverized cheonnyeoncho to prepare a cheonnyeoncho solid; And (c) adding distilled water to the solid material of Cheonnyeoncho, to prepare a Cheonnyeoncho extract containing antioxidant carbon nanoparticles. It provides a method for producing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles using microwaves.

Cheonnyeoncho (Opuntia humifusa, Eastern prickly pear) is a cactus that can winter in inland among the Opuntia sp. The cheonnyeoncho has a shape similar to the palm of the hand, so it is also called a palm cactus.

The cheonnyeoncho is a fleshy perennial plant, that is, a perennial flowering plant, and the stem is fleshy like a general cactus, and has several points of constriction like a node. They are also classified according to their shape. The ganglia has a hetmul tube, a vascular bundle near the epidermis, and the leaves on the shoots are shaped like needles, and are fleshy, and fall after they are fully grown. Cotyledons are lanceolate or triangular, and have the same flesh, and have different flowers and bracts. In general, it is known that the cheonnyeoncho reduces its own moisture to less than half, so that wintering is possible without freezing and dying even at -20°C, thereby maintaining vitality.

Cheonnyeoncho is known to have the effect of treating constipation, diuretic effect, activating bowel movement, and increasing appetite through conventional Dongchogangmok, etc., and it has antioxidant activity, anti-obesity activity, and liver protection effect using Cheonnyeoncho stem. Is known. Studies on these cheonnyeonchos have been mainly conducted on stems and fruits, especially stems, and as a result of these studies, the flesh and stems of cheonnyeonchos are sold as food. As described above, since Cheonnyeoncho, which has been used in various ways since ancient times, has been used as a food or medicine, its safety is evaluated to be guaranteed.

In the step (a), preferably, leaves, fruits, and roots of Cheonnyeoncho may be used.

The step (b) is a step of preparing a solid material of cheonnyeoncho by irradiating microwaves on the pulverized cheonnyeoncho.

The fruits of Cheonnyeoncho contain a large amount of viscous substances of high viscosity, and the composition of this viscous substance is pectin, a polymer dietary fiber composed of arabinose, galactose, xylose, galacturonic acid, and rhamnose.

It is reported that the viscous material is relatively stable to heat and maintains the stability of physical properties and red pigment under acidic conditions, but it is difficult to use Cheonnyeoncho itself as a food material due to the viscous material of high viscosity.

According to an embodiment of the present invention, when microwaves are irradiated to the mixture of Cheonnyeoncho, the viscosity of Cheonnyeoncho due to pectin may be reduced.

The microwave irradiation refers to a thermal reaction in which the extract of Cheonnyeoncho is irradiated with microwaves and heated. The microwave may be a radio wave having a wavelength of 1 m to 1 mm, or a file having a frequency of 300 MHz to 300 GHz. Heating by microwave may vary depending on the amount or state of the reactants.

As an example, the microwave irradiation may be performed at a temperature of 80 to 120 °C.

In addition, the microwave irradiation may be performed for 3 to 15 minutes.

In addition, the microwave irradiation may be performed under normal pressure.

The microwave irradiation may be irradiated in a neutral solution, for example, an aqueous solution. The term “aqueous solution” refers to a solution containing water, which may include water or water and ethanol.

The output of the microwave may be appropriately increased or decreased depending on the amount of the reactant, but may preferably be 300 to 800 W. If it is less than 300 W, it takes a long time to prepare a solid material of Cheonnyeoncho, and if it exceeds 800 W, it is not produced as a solid material (cookie form), and an excessive carbonization process proceeds, so it is preferable to irradiate within the above range.

In step (c), the solid substance of Cheonnyeoncho is extracted by a solvent extraction method to prepare a Cheonnyeoncho extract. The solvent extraction may be any one solvent selected from the group consisting of distilled water, an organic solvent, and a mixture thereof, and it is preferable to use distilled water.

The organic solvent may be an alcohol having 1 to 5 carbon atoms, a polar solvent such as dilute alcohol, ethyl acetate or acetone, and a non-polar solvent of ether, chloroform, benzene, hexane, or dichloromethane, or a mixed solvent thereof. The alcohol having 1 to 5 carbon atoms may be a glycol, such as a dihydric alcohol such as ethylene glycol, propylene glycol, butylene glycol, or pinacol, or a monohydric alcohol such as methanol, ethanol, propanol, butanol, and isopropanol, and an organic solvent is used. If so, it is preferable to use ethanol.

The ethanol may be diluted in water at 10% (v/v) to 99.9% (v/v), but it is preferable to use ethanol diluted at 80% (v/v).

The viscosity of the Cheonnyeoncho extract extracted by the above step may be 1.0 to 3.0 mPa.S.

According to another aspect of the present invention, the present invention comprises the steps of: (a) pulverizing cheonnyeoncho; (b) irradiating microwaves on the pulverized cheonnyeoncho to prepare a cheonnyeoncho solid; And (c) adding distilled water to the solid material of Cheonnyeoncho to provide a cosmetic composition comprising a Cheonnyeoncho extract as an active ingredient comprising the step of preparing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles.

The overlapping description of the method for preparing the Cheonnyeoncho extract will be omitted.

The cosmetic composition according to the present invention preferably contains a microwave extract of Cheonnyeoncho in an amount of 0.001 to 1.0% by weight based on the total weight of the composition.

The cosmetic composition of the present invention is a lotion, lotion, cream, essence, pack, makeup base, foundation, body oil, body lotion, cleansing oil, cleansing foam by adding various known ingredients using a method known in the art. , Hair oil, hair shampoo and hair rinse can be prepared in any one formulation selected from.

Specifically, when the formulation of the cosmetic composition of the present invention is a paste, cream, gel, or pack, known ingredients include animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, Silica, talc, or zinc oxide may be used.

When the formulation of the cosmetic composition of the present invention is a powder, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent may be used, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or non-propionic acid ester of sorbitan may be used.

When the formulation of the cosmetic composition of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene srbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, and the like may be used.

According to another aspect of the present invention, the present invention comprises the steps of: (a) pulverizing cheonnyeoncho; (b) irradiating microwaves on the pulverized cheonnyeoncho to prepare a cheonnyeoncho solid; And (c) by adding distilled water to the solid material of the cheonnyeoncho, preparing a cheonnyeoncho extract containing antioxidant carbon nanoparticles; provides a food composition comprising the cheonnyeoncho extract as an active ingredient.

The food is a health supplement food, a health functional food, a functional food, etc., but is not limited thereto, and includes adding the microwave extract of the present invention to natural foods, processed foods, and general food materials.

Food containing the microwave extract of Cheonnyeoncho according to the present invention may be added as it is or used with other foods or food compositions, and may be appropriately used according to a conventional method. For example, the cheonnyeoncho extract according to the present invention may be added in an amount of 0.1 to 90 parts by weight, preferably 1 to 60 parts by weight, based on 100 parts by weight of raw materials for food or beverage when preparing food or beverage.

There is no particular limitation on the type of the food. The food containing the Cheonnyeoncho extract can be used in the form of preparations for oral administration such as tablets, hard or soft capsules, solutions, suspensions, etc., and these preparations are acceptable conventional carriers, for example, preparations for oral administration. In this case, it may be prepared using an excipient, a binder, a disintegrant, a lubricant, a solubilizing agent, a suspending agent, a preservative or a bulking agent.

Examples of foods to which the Cheonnyeoncho extract can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, and drinks. , Alcoholic beverages and vitamin complexes, and other nutritional supplements, but are not limited to these types of foods.

Example

Hereinafter, examples will be described in detail in order to describe the present invention in detail. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided to more completely describe the present invention to those of ordinary skill in the art.

Comparative Example 1: Preparation of Cheonnyeoncho extract

The leaves, fruits, and roots of Cheonnyeoncho were collected, thorns were removed, and then washed thoroughly with water. Then, it was finely cut into 1 cm or less, and then put into a grinder and pulverized evenly. The pulverized Cheonnyeoncho and 80% ethanol were added to the extractor at a ratio of 1:9 and extraction was performed for 90 minutes while maintaining a pressure of 0.5 atm or less. After the extracted liquid was filtered through a filter for 3 times, water was further evaporated in a concentrator, and 2.5% of a natural preservative was mixed as a preservation stabilizer to finally complete the Cheonnyeoncho extract.

Example 1: Preparation of microwave extract of Cheonnyeoncho

17 g of the raw material of Cheonnyeoncho was subjected to microwave heating treatment at a temperature of 100° C. at 800 w (frequency 2450 MHz) using a microwave oven for 5 minutes. 15 ml of distilled water was added to the microwave-heated cheonnyeoncho solids to obtain the same volume as the cheonnyeoncho extract of Comparative Example 1, and the supernatant was used.

Example 2: Preparation of microwave extract of Cheonnyeoncho

A microwave extract was prepared in the same manner as in Example 1, except that the microwave heat treatment was performed for 10 minutes.

Example 3: Preparation of microwave extract of Cheonnyeoncho

A microwave extract was prepared in the same manner as in Example 1, except that the microwave heat treatment was performed for 15 minutes.

Example 4: Preparation of microwave extract of Cheonnyeoncho

A microwave extract of Cheonnyeoncho was prepared in the same manner as in Example 1, except that the microwave heat treatment was performed for 20 minutes.

Experimental example One: Millennium Viscosity evaluation according to extraction method

The viscosity of the Cheonnyeoncho extract according to Examples 1 to 4 and the Cheonnyeoncho extract of Comparative Example 1 was compared.

1 is a photograph showing the extract of Cheonnyeoncho according to Comparative Example 1 and Examples 1 to 4, and FIG. 2 is a photograph comparing the viscosity of the extract of Cheonnyeoncho according to Comparative Example 1 and Examples 1 to 4.

Referring to Figures 1 and 2, it was confirmed that the extract of Comparative Example 1 without microwave treatment exhibits a high viscosity. On the other hand, in the case of the extracts of Cheonnyeoncho of Examples 1 to 4 subjected to microwave treatment, it was confirmed that the sediment and the supernatant were separated and the viscosity decreased.

Accordingly, it was confirmed that the viscosity of Cheonnyeoncho was significantly reduced when extracted by microwave treatment, and the longer the microwave treatment time was, the more effective it was in reducing the viscosity of the extract.

Experimental example 2: Millennium Within the extract Carbon nanoparticles Confirmation experiment

3 is a photograph showing the result of irradiating ultraviolet rays on the extract of Cheonnyeoncho according to Example 2.

Since carbon nanomaterials exhibit blue-based fluorescence when exposed to ultraviolet rays, ultraviolet rays (UV) were irradiated on the Cheonnyeoncho extract to confirm whether antioxidant carbon nanoparticles were contained in the Cheonnyeoncho extract according to Example 2.

Referring to FIG. 3, it can be seen that blue fluorescence appears as a result of irradiation with ultraviolet rays on the Cheonnyeoncho extract, and according to the present invention, carbon nanoparticles having an antioxidant effect of Cheonnyeoncho can be obtained from the Cheonnyeoncho extract.

Experimental example 3: Millennium Microwave extract HaCaT Evaluation of cytotoxicity on keratinocytes

The cytotoxicity of the microwave extract of Cheonnyeoncho to HaCaT keratinocytes was evaluated. 5 × 10 ⁴ HaCaT cells were added to a 96 well plate and cultured, and the Cheonnyeoncho extract of Example 2 was inoculated into each well at concentrations (10, 50, 100, 300, 500 μg/mL). After incubation for 48 hours, it was washed once with DMEM medium without FBS. On the other hand, as a negative control group, the extract of Cheonnyeoncho of Comparative Example 1 was treated with HaCaT cells and tested.

The HaCaT cells treated with the Cheonnyeoncho extract were treated with 10 µl of CCK-8 assay solution per well, and after 1 to 4 hours, the absorbance was measured at 540 nm using a spectrophotometer to calculate the cell viability.

4 is a graph showing the results of a cytotoxicity experiment when the Cheonnyeoncho extract according to Comparative Examples 1 and 2 was treated on HaCaT cells for 24 hours, and FIG. 5 is a graph showing the results of the Cheonnyeoncho extract according to Comparative Examples 1 and 2 This is a graph showing the results of cytotoxicity experiments when HaCaT cells were treated for 48 hours.

4 and 5, the cell viability of the Cheonnyeoncho extract of Comparative Example 1 gradually decreased as the treatment concentration increased, and when the Cheonnyeoncho extract was treated at a concentration of 500 μg/mL, the cell survival rate was 40%. It was confirmed that it exhibits toxicity to keratinocytes.

On the other hand, the microwave extract of Cheonnyeoncho of Example 2 maintained a certain level of cell viability or higher even when the concentration was increased. Compared to the case where the Cheonnyeoncho extract of Comparative Example 1 was treated at a concentration of 500 μg/mL, when the Cheonnyeoncho extract of Example 2 was treated at the same concentration, the cell survival rate was 70% or more. It was confirmed that the cytotoxicity was low.

Experimental example 4: Millennium Microwave extract HaCaT Evaluation of the degree of apoptosis on keratinocytes

In order to confirm the amount of apoptotic cells generated through UVB-induced apoptosis, it was confirmed by flow cytometry using annexin-V/PI.

HaCaT cells were cultured in a 60 mm dish, and after irradiation with UVB, the untreated Cheonnyeoncho extract and the Cheonnyeoncho microwave extract of Example 2 were treated at different concentrations (100, 300 μg/mL). After 6 hours, pipetting the cell line into a ^{test tube as much as 1×10 6} cells/ml and washing with PBS, discard the supernatant and add a binding buffer (10 mM Hepes/NaOH pH 7.4, 140 mM NaCl, It was pipetted again with 2.5 mM CaCl _{2 ).} Thereafter, FITC-conjugated annexin V Apoptosis Detection Kit (BD Pharmigen, 556547, USA) and propiium Ioddide (PI) (including FITC annexin V kit) were added and incubated for 15 minutes in the dark for staining. Flow cytometer; Fluorescence-activated cell sorting, FACS calibur; Becton-Dickinson), and the percentage of apoptotic cells was measured using the software (Cell Quest software).

6 is a graph comparing the results of apoptosis when the Cheonnyeoncho extract according to Comparative Examples 1 and 2 was treated on HaCaT cells for 24 hours, and FIG. 7 is a graph comparing the Cheonnyeoncho extract according to Comparative Examples 1 and 2 This is a graph comparing the results of apoptosis when treated with HaCaT cells for 48 hours.

Live cell (%) Apoptosis cell (%) Late apoptosis cell (%) Necrosis cell (%) Positive control 1.3 61.7 26.3 10.7 Negative control 91.2 6.8 1.9 0 Example 2 (100 μg/mL) 88.2 8.6 3.1 0 Example 2 (300 μg/mL) 71.4 26.7 1.8 0.1 Comparative Example 1 (100 μg/mL) 54.2 41.7 4.0 0.1 Comparative Example 1 (300 μg/mL) 33.8 60.8 5.4 0.1

Live cell (%) Apoptosis cell (%) Late apoptosis cell (%) Necrosis cell (%) Positive control 1.3 61.7 26.3 10.7 Negative control 91.2 6.8 1.9 0 Example 2 (100 μg/mL) 62.6 35.4 1.8 0.2 Example 2 (300 μg/mL) 51.6 43.6 4.7 0 Comparative Example 1 (100 μg/mL) 16.1 78.8 5 0.1 Comparative Example 1 (300 μg/mL) 13.5 78.3 8 0.3

Tables 1 and 2 are tables summarizing the cell death results when the Cheonnyeoncho extracts of Comparative Examples 1 and 2 were treated with HaCaT keratinocytes.

6 to 7 and Tables 1 to 2, the microwave extract of Cheonnyeoncho of Example 2 inhibits cell death due to UVB of apoptosis cells compared to the extract of Cheonnyeoncho of Comparative Example 1, thereby increasing the viability of cells in a concentration-dependent manner. I could see the facts. In addition, it was confirmed that the cell death rate significantly increased as the exposure time by the Cheonnyeoncho extract in HaCaT keratinocytes increased.

Until now, specific examples of a method for preparing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles using microwaves according to an embodiment of the present invention have been described, but various implementation modifications within the limit not departing from the scope of the present invention This possibility is self-evident.

Therefore, the scope of the present invention should not be defined by being limited to the described embodiments, and should be defined by the claims and equivalents as well as the claims to be described later.

That is, it should be understood that the above-described embodiments are illustrative in all respects and not limiting, and the scope of the present invention is indicated by the claims to be described later rather than the detailed description, and the meaning and scope of the claims and All changes or modified forms derived from the equivalent concept should be construed as being included in the scope of the present invention.

Claims (8)

(a) pulverizing Cheonnyeoncho;
(b) irradiating microwaves on the pulverized cheonnyeoncho to prepare a cheonnyeoncho solid; And
(c) adding distilled water to the solid material of Cheonnyeoncho to prepare an extract of Cheonnyeoncho containing antioxidant carbon nanoparticles;
Method for producing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles using a microwave comprising a. The method of claim 1,
In step (a)
A method for producing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles using microwaves, characterized in that the leaves, fruits, and roots of Cheonnyeoncho are used. The method of claim 1,
In step (b)
The microwave irradiation method for producing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles using microwave, characterized in that carried out at a temperature of 80 to 120 ℃. The method of claim 1,
In step (b)
The microwave irradiation is a method for producing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles using microwave, characterized in that performed for 3 to 15 minutes. The method of claim 1,
In step (b)
Method for producing a Cheonnyeoncho extract containing antioxidant carbon nanoparticles using microwave, characterized in that irradiating the microwave with 300 to 800 W. The method of claim 1,
The cheonnyeoncho extract is a method for producing a cheonnyeoncho extract containing antioxidant carbon nanoparticles using microwave, characterized in that it has a viscosity of 1.0 to 3.0 mPa.S. A cosmetic composition comprising an extract of Cheonnyeoncho prepared by the method of any one of claims 1 to 6 as an active ingredient. A food composition comprising the extract of Cheonnyeoncho prepared by the method of any one of claims 1 to 6 as an active ingredient.

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