KR20210014441A - Method for producing soy sauce using natural food materials - Google Patents

Method for producing soy sauce using natural food materials Download PDF

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KR20210014441A
KR20210014441A KR1020190092526A KR20190092526A KR20210014441A KR 20210014441 A KR20210014441 A KR 20210014441A KR 1020190092526 A KR1020190092526 A KR 1020190092526A KR 20190092526 A KR20190092526 A KR 20190092526A KR 20210014441 A KR20210014441 A KR 20210014441A
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extract
soy sauce
savory
weight
cumin
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KR1020190092526A
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Korean (ko)
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KR102334733B1 (en
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진종식
이광진
이기원
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전북대학교산학협력단
옹고집영농조합법인
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/50Soya sauce
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof

Abstract

The present invention relates to a method for manufacturing seasoned soy sauce suitable for preference of consumers, and seasoned soy sauce manufactured thereby. According to the present invention, the method comprises the following steps: (1) adding onion skins, black beans, kelp, and shiitake mushrooms to water and boiling and filtering the mixture to manufacture a seasoning solution; (2) adding water to green tea leaves, basil, savory, ginkgo leaves, and cumin, respectively, and extracting and filtering each mixture to manufacture a green tea extract, a basil extract, a savory extract, a ginkgo leaf extract, and a cumin extract; (3) mixing the green tea extract, the basil extract, the savory extract, the ginkgo leaf extract, and the cumin extract manufactured in the step (2) to manufacture a mixed extract; and (4) mixing the seasoning solution manufactured in the step (1), the mixed extract manufactured in the step (3), soy sauce, fish sauce, sugar, Japanese apricot syrup, apples, pears, onions, starch syrup, and garlic with respect to the total weight of seasoned soy sauce, and boiling and filtering the mixture.

Description

천연식품소재를 함유하는 맛간장의 제조방법{Method for producing soy sauce using natural food materials}Method for producing taste soy sauce containing natural food materials {Method for producing soy sauce using natural food materials}

본 발명은 (1) 물에 양파껍질, 검정콩, 다시마 및 표고버섯을 첨가하고 끓인 후 여과하여 조미액을 제조하는 단계; (2) 녹차잎, 바질, 세이보리, 은행잎 및 큐민에 각각 물을 첨가하고 추출한 후 여과하고 농축하여 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 각각 제조하는 단계; (3) 상기 (2)단계의 제조한 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 혼합하여 혼합 추출물을 제조하는 단계; 및 (4) 맛간장 총 중량 기준으로, 상기 (1)단계의 제조한 조미액, 상기 (3)단계의 제조한 혼합 추출물과 진간장, 어간장, 설탕, 매실청, 사과, 배, 양파, 물엿 및 마늘을 혼합하고 끓인 후 여과하는 단계를 포함하여 제조하는 것을 특징으로 하는 맛간장의 제조방법 및 상기 방법으로 제조된 맛간장에 관한 것이다.The present invention comprises the steps of (1) adding onion skin, black beans, kelp and shiitake mushrooms to water, boiling, and filtering to prepare a seasoning solution; (2) adding water to green tea leaves, basil, savory, ginkgo leaves and cumin, respectively, followed by extraction, filtering, and concentrating to prepare green tea extract, basil extract, savory extract, ginkgo leaf extract, and cumin extract, respectively; (3) preparing a mixed extract by mixing the green tea extract, basil extract, savory extract, ginkgo leaf extract and cumin extract prepared in step (2); And (4) based on the total weight of taste soy sauce, the seasoning liquid prepared in step (1), the mixed extract prepared in step (3), and soy sauce, fish soy sauce, sugar, plum green, apple, pear, onion, starch syrup, and garlic. It relates to a method for producing flavored soy sauce, characterized in that it comprises the step of mixing, boiling, and filtering, and to flavored soy sauce prepared by the method.

간장은 콩을 주원료로 하여 발효, 숙성시킨 우리나라의 대표적인 대두 발효식품이다. 간장은 단백질과 아미노산 함량이 높을 뿐만 아니라 저장성이 뛰어나며, 그 특유의 맛과 향을 지니고 있어 우리 조상들의 식생활에 널리 애용되어 왔다. 간장의 숙성과정 중 맛, 향, 색 등의 품질을 결정짓는 데에는 여러 가지 요인이 있으나 크게 원료, 제조방법, 메주 및 이에 적용되는 균주 등으로 나눌 수 있다. 좋은 원료와 숙성과정 중 미생물의 작용에 따라 그 맛과 향 등이 결정되지만, 간장은 제조방법에 따라 품질에 많은 영향을 미친다.Soy sauce is a representative fermented soybean food in Korea that is fermented and aged with soybean as the main ingredient. Soy sauce is not only high in protein and amino acids, but also has excellent storage properties, and has a unique taste and aroma, so it has been widely used in the diet of our ancestors. During the aging process of soy sauce, there are various factors that determine the quality of taste, aroma, and color, but can be largely divided into raw materials, manufacturing methods, meju and strains applied thereto. Although good raw materials and the action of microorganisms during the aging process determine the taste and aroma, the quality of soy sauce depends on the manufacturing method.

그리고, 종래에 시판되는 간장의 경우 염도가 18~19%로서 매우 높은 편이어서 음식에 직접 사용할 경우 강한 짠맛을 부여하게 되며, 염도를 낮출 경우에는 산화가 되어 부패될 우려가 있다. 또한, 기존의 향신 간장의 형태로 시판되거나 요리책에서 제시된 맛간장의 경우는 제조과정에서 영양성분이 파괴되고 염도가 높아지거나 다양한 인공 첨가물에 의하여 향미가 매우 강해지고 건강에도 좋지 않은 문제점이 있었다.And, in the case of conventionally available soy sauce, the salinity of soy sauce is 18 to 19%, which is very high, so when used directly on food, it gives a strong salty taste, and when the salinity is lowered, there is a risk of being oxidized and spoiled. In addition, in the case of flavored soy sauce marketed in the form of conventional spiced soy sauce or presented in cookbooks, nutrients are destroyed during the manufacturing process and saltiness is increased, or the flavor is very strong and unhealthy due to various artificial additives.

한편, 음식 조리시에 간을 맞추기 위해서는 소금이나 간장을 사용하며, 이에 더불어 맛을 더하기 위해서는 각종 양념, 조미료, 파, 양파 등의 부재료를 첨가시킨다. 따라서, 음식의 조리시 각종 양념이나 조미료를 별도로 준비하여야 하는 번거러움과 함께 그 각각의 첨가비율의 산정이 쉽지 않은 문제점이 있으며, 조리시간이 길어지게 되는 단점이 있다.On the other hand, salt or soy sauce is used to season food when cooking, and subsidiary materials such as various seasonings, seasonings, green onions, and onions are added to add flavor. Therefore, there is a problem in that it is difficult to calculate each addition ratio, along with the hassle of separately preparing various seasonings or seasonings when cooking food, and there is a disadvantage in that the cooking time is lengthened.

한국등록특허 제1664268호에는 도라지 맛간장의 제조방법이 개시되어 있고, 한국등록특허 제1924366호에는 흑미 맛간장의 제조방법이 개시되어 있으나, 본 발명의 천연식품소재를 함유하는 맛간장의 제조방법과는 상이하다.Korean Patent No. 1664268 discloses a method of manufacturing bellflower flavored soy sauce, and Korean Registered Patent No. 1924366 discloses a method of manufacturing black rice flavored soy sauce, but a method of manufacturing flavored soy sauce containing natural food ingredients of the present invention It is different from

본 발명은 상기와 같은 요구에 의해 안출된 것으로서, 본 발명의 목적은 다양한 식품 소재를 이용하여 영양성과 기호도가 우수한 새로운 형태의 맛간장을 제조하기 위해, 부재료 선정, 배합비, 추출, 가열 등의 제조조건을 최적화하여 기존의 맛간장에 비해 영양성분이 증진되고 깊은맛과 감칠맛이 우수하여 소비자들의 기호도에 적합한 맛간장의 제조방법을 제공하는 데 있다.The present invention was conceived by the above demands, and an object of the present invention is to manufacture a new type of taste soy sauce with excellent nutritional and palatability using various food materials, such as selection of subsidiary materials, mixing ratio, extraction, heating, etc. By optimizing the conditions, the nutrients are improved compared to the existing taste soy sauce, and its deep and umami taste is excellent, so that it provides a method of manufacturing taste soy sauce suitable for consumers' preference.

상기 과제를 해결하기 위해, 본 발명은 (1) 물에 양파껍질, 검정콩, 다시마 및 표고버섯을 첨가하고 끓인 후 여과하여 조미액을 제조하는 단계; (2) 녹차잎, 바질, 세이보리, 은행잎 및 큐민에 각각 물을 첨가하고 추출한 후 여과하고 농축하여 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 각각 제조하는 단계; (3) 상기 (2)단계의 제조한 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 혼합하여 혼합 추출물을 제조하는 단계; 및 (4) 맛간장 총 중량 기준으로, 상기 (1)단계의 제조한 조미액, 상기 (3)단계의 제조한 혼합 추출물과 진간장, 어간장, 설탕, 매실청, 사과, 배, 양파, 물엿 및 마늘을 혼합하고 끓인 후 여과하는 단계를 포함하여 제조하는 것을 특징으로 하는 맛간장의 제조방법을 제공한다.In order to solve the above problems, the present invention comprises the steps of: (1) adding onion skin, black beans, kelp and shiitake mushrooms to water, boiling, and filtering to prepare a seasoning solution; (2) adding water to green tea leaves, basil, savory, ginkgo leaves and cumin, respectively, followed by extraction, filtering, and concentrating to prepare green tea extract, basil extract, savory extract, ginkgo leaf extract, and cumin extract, respectively; (3) preparing a mixed extract by mixing the green tea extract, basil extract, savory extract, ginkgo leaf extract and cumin extract prepared in step (2); And (4) based on the total weight of taste soy sauce, the seasoning liquid prepared in step (1), the mixed extract prepared in step (3), and soy sauce, fish soy sauce, sugar, plum green, apple, pear, onion, starch syrup, and garlic. It provides a method for producing flavored soy sauce, characterized in that it comprises the step of mixing and boiling and then filtering.

또한, 본 발명은 상기 방법으로 제조된 맛간장을 제공한다.In addition, the present invention provides a taste soy sauce prepared by the above method.

본 발명의 맛간장은 인공적인 색소, 방부제 및 조미료 등은 일체 첨가하지 않고 천연재료만 사용하여 자연 그대로의 맛을 가지면서, 맛이 산뜻하고 깔끔하면서 짜지 않고, 감칠맛이 향상되어 기호도가 우수하여, 각종 국, 찌개, 조림, 무침 등의 다양한 요리에 유용하게 사용할 수 있으며, 항당뇨 활성이 있는 식품 소재를 사용하여 항당뇨 활성이 우수한 맛간장을 제공할 수 있다.Flavored soy sauce of the present invention does not add any artificial colors, preservatives, seasonings, etc., and uses only natural ingredients to have the natural taste, while the taste is refreshing and neat and not salty, and the taste is improved, so that the taste is improved and the preference is excellent. It can be usefully used in various dishes such as soup, stew, stew, and seasoned, and it is possible to provide taste soy sauce with excellent anti-diabetic activity by using food materials with anti-diabetic activity.

도 1은 녹차 추출물의 농도별 처리에 따른 췌장세포 생존률을 비교한 그래프이다.
도 2는 혈중 인슐린 농도를 비교한 그래프이다.
도 2의 맛간장: 혼합 추출물 무첨가 맛간장, 개발 간장: 제조예 1의 맛간장, Mix: 제조예 1의 (3)단계의 혼합 추출물을 의미한다.
1 is a graph comparing pancreatic cell survival rates according to concentrations of green tea extract.
2 is a graph comparing the insulin concentration in blood.
Fig. 2 taste soy sauce: taste soy sauce without mixed extract, developed soy sauce: taste soy sauce in Preparation Example 1, Mix: refers to the mixed extract in step (3) of Preparation Example 1.

본 발명의 목적을 달성하기 위하여, 본 발명은In order to achieve the object of the present invention, the present invention

(1) 물에 양파껍질, 검정콩, 다시마 및 표고버섯을 첨가하고 끓인 후 여과하여 조미액을 제조하는 단계;(1) adding onion skin, black beans, kelp and shiitake mushrooms to water, boiling, and filtering to prepare a seasoning solution;

(2) 녹차잎, 바질, 세이보리, 은행잎 및 큐민에 각각 물을 첨가하고 추출한 후 여과하고 농축하여 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 각각 제조하는 단계;(2) adding water to green tea leaves, basil, savory, ginkgo leaves and cumin, respectively, followed by extraction, filtering, and concentrating to prepare green tea extract, basil extract, savory extract, ginkgo leaf extract, and cumin extract, respectively;

(3) 상기 (2)단계의 제조한 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 혼합하여 혼합 추출물을 제조하는 단계; 및(3) preparing a mixed extract by mixing the green tea extract, basil extract, savory extract, ginkgo leaf extract and cumin extract prepared in step (2); And

(4) 맛간장 총 중량 기준으로, 상기 (1)단계의 제조한 조미액, 상기 (3)단계의 제조한 혼합 추출물과 진간장, 어간장, 설탕, 매실청, 사과, 배, 양파, 물엿 및 마늘을 혼합하고 끓인 후 여과하는 단계를 포함하여 제조하는 것을 특징으로 하는 맛간장의 제조방법을 제공한다.(4) Based on the total weight of taste soy sauce, the seasoning liquid prepared in step (1), the mixed extract prepared in step (3), and soy sauce, fish soy sauce, sugar, plum green, apple, pear, onion, starch syrup, and garlic It provides a method for producing taste soy sauce, characterized in that it comprises the step of mixing, boiling and filtering.

본 발명의 맛간장의 제조방법에서, 상기 (1)단계의 조미액은 바람직하게는 물 80~120 mL에 양파껍질 0.05~0.07 g, 검정콩 1.8~2.2 g, 다시마 0.08~0.12 g 및 표고버섯 0.06~0.08 g을 첨가한 후 90~100℃에서 8~12분 동안 끓인 후 여과하여 제조할 수 있으며, 더욱 바람직하게는 물 100 mL에 양파껍질 0.06 g, 검정콩 2.06 g, 다시마 0.1 g 및 표고버섯 0.07 g을 첨가한 후 100℃에서 10분 동안 끓인 후 여과하여 제조할 수 있다. 상기와 같은 재료 종류, 배합비 및 추출조건으로 제조한 조미액은 풍미 및 감칠맛이 우수하여 맛간장 제조 시 적정량 첨가할 경우 맛간장의 맛을 더욱 풍부하게 하는 역할을 할 수 있다.In the method for producing taste soy sauce of the present invention, the seasoning solution of step (1) is preferably 0.05 to 0.07 g of onion skin, 1.8 to 2.2 g of black beans, 0.08 to 0.12 g of kelp, and 0.06 to shiitake mushrooms in 80 to 120 mL of water. After adding 0.08 g, it can be boiled at 90 to 100°C for 8 to 12 minutes and then filtered. More preferably, 0.06 g of onion peel, 2.06 g of black beans, 0.1 g of kelp, and 0.07 g of shiitake mushrooms are added to 100 mL of water. It can be prepared by adding and boiling for 10 minutes at 100° C. and filtering. The seasoning liquid prepared with the above material types, mixing ratios and extraction conditions has excellent flavor and umami, and thus, when added in an appropriate amount during the production of taste soy sauce, it can serve to enrich the taste of taste soy sauce.

또한, 본 발명의 맛간장의 제조방법에서, 상기 (2)단계는 바람직하게는 녹차잎, 바질, 세이보리, 은행잎 및 큐민에 각각 물을 8~12배(v/w) 첨가한 후 55~65℃에서 4~6시간 동안 추출한 후 여과하고 75~85℃에서 농축하여 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 각각 제조할 수 있으며, 더욱 바람직하게는 녹차잎, 바질, 세이보리, 은행잎 및 큐민에 각각 물을 10배(v/w) 첨가한 후 60℃에서 5시간 동안 추출한 후 여과하고 80℃에서 농축하여 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 각각 제조할 수 있다.In addition, in the method for producing taste soy sauce of the present invention, the step (2) is preferably 55 to 65 after adding 8 to 12 times (v/w) water to green tea leaves, basil, savory, ginkgo leaves and cumin, respectively. After extracting for 4 to 6 hours at °C, filtered and concentrated at 75 to 85 °C to prepare green tea extract, basil extract, savory extract, ginkgo leaf extract and cumin extract, respectively, more preferably green tea leaves, basil, savory, After adding 10 times (v/w) water to each ginkgo leaf and cumin, extracting at 60°C for 5 hours, filtering and concentrating at 80°C to prepare green tea extract, basil extract, savory extract, ginkgo leaf extract and cumin extract, respectively. I can.

또한, 본 발명의 맛간장의 제조방법에서, 상기 (3)단계의 혼합 추출물은 바람직하게는 녹차 추출물 18~22 mL, 바질 추출물 18~22 mL, 세이보리 추출물 8~12 mL, 은행잎 추출물 8~12 mL 및 큐민 추출물 8~12 mL를 혼합하여 제조할 수 있으며, 더욱 바람직하게는 녹차 추출물 20 mL, 바질 추출물 20 mL, 세이보리 추출물 10 mL, 은행잎 추출물 10 mL 및 큐민 추출물 10 mL를 혼합하여 제조할 수 있다.In addition, in the method for producing taste soy sauce of the present invention, the mixed extract of step (3) is preferably 18 to 22 mL of green tea extract, 18 to 22 mL of basil extract, 8 to 12 mL of savory extract, 8 to 12 of ginkgo leaf extract. mL and 8 to 12 mL of cumin extract can be mixed, and more preferably, 20 mL of green tea extract, 20 mL of basil extract, 10 mL of savory extract, 10 mL of ginkgo leaf extract, and 10 mL of cumin extract can be prepared by mixing. have.

상기 (2) 및 (3)의 재료 종류, 추출 및 배합비로 혼합한 혼합 추출물을 적정량 첨가하여 맛간장을 제조하는 것이 맛간장의 감칠맛과 풍미를 더해주고, 천연재료만을 사용하여 친환경적인 맛과 건강지향형의 고부가가치의 맛간장을 소비자들에게 제공할 수 있다.To prepare taste soy sauce by adding an appropriate amount of the mixed extract mixed with the material type, extraction and mixing ratio of the above (2) and (3) adds the umami and flavor of taste soy sauce, and eco-friendly taste and health using only natural ingredients. Oriented, high-value added taste soy sauce can be provided to consumers.

또한, 본 발명의 맛간장의 제조방법에서, 상기 (4)단계는 바람직하게는 맛간장 총 중량 기준으로, 조미액 16~17 중량%, 혼합 추출물 19~21 중량%, 진간장 23~26 중량%, 어간장 1.8~2.4 중량%, 설탕 3~4 중량%, 매실청 1.8~2.4 중량%, 사과 9~10 중량%, 배 9~10 중량%, 양파 9~10 중량%, 물엿 3~4 중량% 및 마늘 0.1~0.3 중량%를 혼합하고 90~100℃에서 4~6분 동안 끓인 후 여과할 수 있으며, 더욱 바람직하게는 맛간장 총 중량 기준으로, 조미액 16.5 중량%, 혼합 추출물 20 중량%, 진간장 24.4 중량%, 어간장 2.1 중량%, 설탕 3.4 중량%, 매실청 2.1 중량%, 사과 9.3 중량%, 배 9.3 중량%, 양파 9.3 중량%, 물엿 3.4 중량% 및 마늘 0.2 중량%를 혼합하고 100℃에서 5분 동안 끓인 후 여과하는 단계를 포함할 수 있다. 상기와 같은 재료 종류, 배합 및 가열조건으로 제조한 맛간장은 재료들이 잘 어우러지면서 맛이 더욱 깊고 단맛, 짠맛 및 감칠맛이 조화를 이룬 맛간장으로 제조할 수 있었다.In addition, in the manufacturing method of taste soy sauce of the present invention, step (4) is preferably based on the total weight of taste soy sauce, seasoning liquid 16 to 17 wt%, mixed extract 19 to 21 wt%, soy sauce 23 to 26 wt%, Fish soy sauce 1.8-2.4% by weight, sugar 3-4% by weight, plum green 1.8-2.4% by weight, apple 9-10% by weight, pear 9-10% by weight, onion 9-10% by weight, starch syrup 3-4% by weight and 0.1 to 0.3% by weight of garlic may be mixed and boiled at 90 to 100°C for 4 to 6 minutes, and then filtered. More preferably, based on the total weight of taste soy sauce, seasoning liquid 16.5% by weight, mixed extract 20% by weight, and soy sauce 24.4 Mixing% by weight, 2.1% by weight of fish soy sauce, 3.4% by weight of sugar, 2.1% by weight of plum green, 9.3% by weight of apples, 9.3% by weight of pears, 9.3% by weight of onion, 3.4% by weight of starch syrup, and 0.2% by weight of garlic, and 5 at 100°C After boiling for a minute, it may include filtering. Flavored soy sauce prepared with the above material types, formulations and heating conditions could be prepared as flavored soy sauce in which the ingredients were well harmonized and the taste was deeper, and the sweet, salty and umami tastes were harmonized.

본 발명의 맛간장의 제조방법은, 보다 구체적으로는The method for producing taste soy sauce of the present invention, more specifically

(1) 물 80~120 mL에 양파껍질 0.05~0.07 g, 검정콩 1.8~2.2 g, 다시마 0.08~0.12 g 및 표고버섯 0.06~0.08 g을 첨가한 후 90~100℃에서 8~12분 동안 끓인 후 여과하여 조미액을 제조하는 단계;(1) After adding 0.05 to 0.07 g of onion skin, 1.8 to 2.2 g of black beans, 0.08 to 0.12 g of kelp, and 0.06 to 0.08 g of shiitake mushrooms in 80 to 120 mL of water, boil at 90 to 100°C for 8 to 12 minutes. Filtering to prepare a seasoning liquid;

(2) 녹차잎, 바질, 세이보리, 은행잎 및 큐민에 각각 물을 8~12배(v/w) 첨가한 후 55~65℃에서 4~6시간 동안 추출한 후 여과하고 75~85℃에서 농축하여 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 각각 제조하는 단계;(2) Add 8 to 12 times (v/w) water to green tea leaves, basil, savory, ginkgo leaves and cumin, respectively, extract at 55 to 65°C for 4 to 6 hours, filter, and concentrate at 75 to 85°C. Preparing green tea extract, basil extract, savory extract, ginkgo leaf extract, and cumin extract, respectively;

(3) 상기 (2)단계의 제조한 녹차 추출물 18~22 mL, 바질 추출물 18~22 mL, 세이보리 추출물 8~12 mL, 은행잎 추출물 8~12 mL 및 큐민 추출물 8~12 mL를 혼합하여 혼합 추출물을 제조하는 단계; 및(3) Mixed extract by mixing 18 to 22 mL of green tea extract prepared in step (2), 18 to 22 mL of basil extract, 8 to 12 mL of savory extract, 8 to 12 mL of ginkgo leaf extract, and 8 to 12 mL of cumin extract Manufacturing a; And

(4) 맛간장 총 중량 기준으로, 상기 (1)단계의 제조한 조미액 16~17 중량%, 상기 (3)단계의 제조한 혼합 추출물 19~21 중량%와 진간장 23~26 중량%, 어간장 1.8~2.4 중량%, 설탕 3~4 중량%, 매실청 1.8~2.4 중량%, 사과 9~10 중량%, 배 9~10 중량%, 양파 9~10 중량%, 물엿 3~4 중량% 및 마늘 0.1~0.3 중량%를 혼합하고 90~100℃에서 4~6분 동안 끓인 후 여과하는 단계를 포함할 수 있으며,(4) Based on the total weight of taste soy sauce, 16 to 17% by weight of seasoning liquid prepared in step (1), 19 to 21% by weight of mixed extract prepared in step (3) and 23 to 26% by weight of soy sauce, fish soy sauce 1.8 ~2.4 wt%, sugar 3~4 wt%, plum green 1.8~2.4 wt%, apple 9-10 wt%, pear 9-10 wt%, onion 9-10 wt%, starch syrup 3-4 wt% and garlic 0.1 Mixing ~0.3% by weight, boiling for 4-6 minutes at 90-100°C, and then filtering,

더욱 구체적으로는More specifically

(1) 물 100 mL에 양파껍질 0.06 g, 검정콩 2.06 g, 다시마 0.1 g 및 표고버섯 0.07 g을 첨가한 후 100℃에서 10분 동안 끓인 후 여과하여 조미액을 제조하는 단계;(1) adding 0.06 g of onion skin, 2.06 g of black beans, 0.1 g of kelp, and 0.07 g of shiitake mushrooms to 100 mL of water, boiling at 100°C for 10 minutes, and filtering to prepare a seasoning solution;

(2) 녹차잎, 바질, 세이보리, 은행잎 및 큐민에 각각 물을 10배(v/w) 첨가한 후 60℃에서 5시간 동안 추출한 후 여과하고 80℃에서 농축하여 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 각각 제조하는 단계;(2) After adding 10 times (v/w) water to green tea leaves, basil, savory, ginkgo leaves and cumin, extracting for 5 hours at 60°C, filtering and concentrating at 80°C, green tea extract, basil extract, savory extract , Preparing each of the ginkgo leaf extract and the cumin extract;

(3) 상기 (2)단계의 제조한 녹차 추출물 20 mL, 바질 추출물 20 mL, 세이보리 추출물 10 mL, 은행잎 추출물 10 mL 및 큐민 추출물 10 mL를 혼합하여 혼합 추출물을 제조하는 단계; 및(3) preparing a mixed extract by mixing 20 mL of green tea extract prepared in step (2), 20 mL of basil extract, 10 mL of savory extract, 10 mL of ginkgo leaf extract and 10 mL of cumin extract; And

(4) 맛간장 총 중량 기준으로, 상기 (1)단계의 제조한 조미액 16.5 중량%, 상기 (3)단계의 제조한 혼합 추출물 20 중량%와 진간장 24.4 중량%, 어간장 2.1 중량%, 설탕 3.4 중량%, 매실청 2.1 중량%, 사과 9.3 중량%, 배 9.3 중량%, 양파 9.3 중량%, 물엿 3.4 중량% 및 마늘 0.2 중량%를 혼합하고 100℃에서 5분 동안 끓인 후 여과하는 단계를 포함할 수 있다.(4) Based on the total weight of taste soy sauce, 16.5% by weight of the seasoning liquid prepared in step (1), 20% by weight of the mixed extract prepared in step (3) and 24.4% by weight of soy sauce, 2.1% by weight of fish soy sauce, and 3.4% sugar %, 2.1% by weight of plum, 9.3% by weight of apples, 9.3% by weight of pears, 9.3% by weight of onion, 3.4% by weight of starch syrup, and 0.2% by weight of garlic are mixed, boiled at 100°C for 5 minutes, and then filtered. have.

본 발명은 또한, 상기 방법으로 제조된 맛간장을 제공한다.The present invention also provides a taste soy sauce prepared by the above method.

이하, 본 발명의 제조예 및 실시예를 들어 상세히 설명한다. 단, 하기 제조예 및 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 제조예 및 실시예에 한정되는 것은 아니다.Hereinafter, preparation examples and examples of the present invention will be described in detail. However, the following Preparation Examples and Examples are only illustrative of the present invention, and the content of the present invention is not limited to the following Preparation Examples and Examples.

제조예Manufacturing example 1. One. 맛간장Soy sauce

(1) 물 100 mL에 양파껍질 0.06 g, 검정콩 2.06 g, 다시마 0.1 g 및 표고버섯 0.07 g을 첨가한 후 100℃에서 10분 동안 끓인 후 여과하여 조미액을 제조하였다.(1) 0.06 g of onion skin, 2.06 g of black beans, 0.1 g of kelp, and 0.07 g of shiitake were added to 100 mL of water, and then boiled at 100° C. for 10 minutes and filtered to prepare a seasoning solution.

(2) 녹차잎, 바질(basil), 세이보리(savory), 은행잎 및 큐민(cumin)에 각각 정제수를 10배(v/w) 첨가한 후 60℃에서 5시간 동안 추출한 후 필터 페이퍼(Advantec, No. 2)에 필터한 후 1/10 부피가 될 때까지 80℃에서 감압 농축하여 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 각각 제조하였다.(2) After 10 times (v/w) of purified water was added to green tea leaves, basil, savory, ginkgo leaves and cumin, respectively, extracted for 5 hours at 60°C, and filter paper (Advantec, No. After filtering in 2), it was concentrated under reduced pressure at 80° C. until 1/10 volume, to prepare green tea extract, basil extract, savory extract, ginkgo biloba extract and cumin extract, respectively.

(3) 상기 (2)단계의 제조한 녹차 추출물 20 mL, 바질 추출물 20 mL, 세이보리 추출물 10 mL, 은행잎 추출물 10 mL 및 큐민 추출물 10 mL를 혼합하여 혼합 추출물을 제조하였다.(3) A mixed extract was prepared by mixing 20 mL of the green tea extract prepared in step (2), 20 mL of basil extract, 10 mL of savory extract, 10 mL of ginkgo leaf extract, and 10 mL of cumin extract.

(4) 맛간장 총 중량 기준으로, 상기 (1)단계의 제조한 조미액 16.5 중량%, 상기 (3)단계의 제조한 혼합 추출물 20 중량%와 진간장(옹고집 조선간장) 24.4 중량%, 어간장(옹고집 어간장) 2.1 중량%, 설탕 3.4 중량%, 매실청(참존식품) 2.1 중량%, 사과 9.3 중량%, 배 9.3 중량%, 양파 9.3 중량%, 물엿 3.4 중량% 및 다진 마늘 0.2 중량%를 혼합한 후 100℃에서 5분 동안 끓인 후 여과하였다.(4) Based on the total weight of taste soy sauce, 16.5% by weight of the seasoning liquid prepared in step (1), 20% by weight of the mixed extract prepared in step (3) and 24.4% by weight of Jin Soy Sauce (Ongojip Chosun Soy Sauce), Fish Soy Sauce Fish soy sauce) 2.1% by weight, sugar 3.4% by weight, plum green (charmzone food) 2.1% by weight, apple 9.3% by weight, pear 9.3% by weight, onion 9.3% by weight, starch syrup 3.4% by weight, and minced garlic 0.2% by weight After boiling for 5 minutes at 100 ℃ filtered.

조미액 및 혼합 추출물 배합비Mixing ratio of seasoning liquid and mixed extract 재료 종류Material type 제조예 1Manufacturing Example 1 비교예 1Comparative Example 1 비교예 2Comparative Example 2 비교예 3Comparative Example 3 조미액Seasoning water 100 mL100 mL 100 mL100 mL 100 mL100 mL 100 mL100 mL 양파껍질Onion peel 0.06 g0.06 g 0.76 g0.76 g 0.06 g0.06 g 0.19 g0.19 g 검정콩Black beans 2.06 g2.06 g -- 1.86 g1.86 g 1.7 g1.7 g 다시마Kelp 0.1 g0.1 g 0.76 g0.76 g 0.1 g0.1 g 0.2 g0.2 g 표고버섯Shiitake mushrooms 0.07 g0.07 g 0.76 g0.76 g 0.07 g0.07 g 0.2 g0.2 g 마늘garlic -- -- 0.1 g0.1 g -- radish -- -- 0.1 g0.1 g -- 혼합 추출물Mixed extract 녹차 추출물Green tea extract 20 mL20 mL 40 mL40 mL 10 mL10 mL 10 mL10 mL 바질 추출물Basil extract 20 mL20 mL 40 mL40 mL 10 mL10 mL 15 mL15 mL 세이보리 추출물Savory extract 10 mL10 mL -- 10 mL10 mL 5 mL5 mL 은행잎 추출물Ginkgo biloba extract 10 mL10 mL -- 10 mL10 mL 20 mL20 mL 큐민 추출물Cumin extract 10 mL10 mL 20 mL20 mL 10 mL10 mL 20 mL20 mL 로즈마리 추출물Rosemary extract -- -- 10 mL10 mL -- 대추 추출물Jujube extract -- -- 10 mL10 mL --

비교예Comparative example 1 내지 3. 1 to 3. 맛간장Soy sauce

상기 제조예 1의 방법으로 맛간장을 제조하되, 상기 (1)단계의 조미액과 (3)단계의 혼합 추출물 제조 시 상기 표 1의 재료 및 배합비로 제조한 조미액 및 혼합 추출물을 이용하여 비교예 1 내지 3의 맛간장을 각각 제조하였다.To prepare taste soy sauce by the method of Preparation Example 1, Comparative Example 1 using the seasoning liquid and mixed extract prepared with the ingredients and blending ratio of Table 1 when preparing the seasoning liquid in step (1) and the mixed extract in step (3). Each of the taste soy sauce of 3 was prepared.

비교예Comparative example 4 및 5. 4 and 5. 맛간장Soy sauce

상기 제조예 1의 방법으로 맛간장을 제조하되, 상기 (4)단계의 재료 배합시 하기 표 2의 재료 및 배합비로 배합하여 비교예 4 및 5의 맛간장을 각각 제조하였다.Flavored soy sauce was prepared by the method of Preparation Example 1, but the taste soy sauce of Comparative Examples 4 and 5 was prepared by mixing with the ingredients and mixing ratios of Table 2 below when the ingredients in step (4) were mixed.

재료 배합비(중량%)Material mixing ratio (% by weight) 재료 종류Material type 제조예 1Manufacturing Example 1 비교예 4Comparative Example 4 비교예 5Comparative Example 5 조미액Seasoning 16.516.5 16.516.5 19.519.5 혼합 추출물Mixed extract 2020 2020 1515 진간장Soy sauce 24.424.4 24.424.4 2020 어간장Fish soy sauce 2.12.1 2.12.1 66 설탕Sugar 3.43.4 3.43.4 22 매실청Plum Cheong 2.12.1 2.12.1 44 사과Apple 9.39.3 9.39.3 88 ship 9.39.3 9.39.3 1212 양파onion 9.39.3 4.34.3 1212 물엿corn syrup 3.43.4 1.41.4 1One 마늘garlic 0.20.2 0.20.2 0.50.5 대파Green Onion -- 55 -- 감초licorice -- 22 --

실험방법Experiment method

1. 세포보호 효과1. Cell protection effect

녹차 추출물의 췌장세포에 대한 보호 효과를 확인하기 위하여, 한국세포주은행에서 분양받은 췌장 세포인 RINm5F 세포 및 MIN6 세포와 위 5종 추출물을 처리하여 세포 보호 효과 정도를 시판되는 분석 키트를 이용하여 확인하였다. 녹차잎 추출물을 10 ㎍/mL~100 mg/mL의 농도로 배양 배지에 첨가하여 시료를 제조하였다. 그리고, RINm5F 세포를 10% FBS, 1% 페니실린-스트렙토마이신을 첨가한 RPMI 배지를 포함하는 96-웰 플레이트 내에 5×104으로 분주하여 37℃, 5% CO2가 유지되도록 배양하였다. MIN6 세포는 10% FBS, 1% 페니실린-스트렙토마이신, 1% 55 mM 2-메르캅토에탄올(2-Mercaptoethanol)을 첨가한 DMEM 배지를 포함하는 96-웰 플레이트 내에서 37℃, 5% CO2가 유지되도록 배양하였다. 약 1일 동안 RINm5F 세포, MIN6 세포를 안정화시킨 뒤, 상기 제조한 추출물 시료를 96 웰 플레이트의 한 웰 볼륨 200 ㎕의 100분의 1의 농도인 2 ㎕씩 첨가하였다. 4시간 후 RINm5F 세포에 STZ(Streptozotocin) 100 mM의 농도로 96 웰 플레이트의 한 웰 볼륨 200 ㎕의 100분의 1의 농도인 2 ㎕씩 첨가하였다. 그리고 MIN6 세포에 STZ(Streptozotocin) 300 mM의 농도로 96 웰 플레이트의 한 웰 볼륨 200 ㎕의 100분의 1의 농도인 2 ㎕씩 첨가하였다. 24시간 후 RINm5F 세포, MIN6 세포에 CCK 96-웰 플레이트의 한 웰 볼륨 200 ㎕의 20분의 1의 농도인 10 ㎕씩 각 웰에 첨가하였다. 4시간 정도 37℃, 5% CO2가 유지된 인큐베이터에서 반응시켜 준 뒤 플레이트 리더를 이용하여 540 nm에서 흡광도를 측정하였다.In order to confirm the protective effect of green tea extract on pancreatic cells, the degree of cytoprotective effect was confirmed using a commercially available assay kit by treating the pancreatic cells, RINm5F cells and MIN6 cells, and the above five types of extracts, which are pancreatic cells distributed from the Korea Cell Line Bank. . A sample was prepared by adding green tea leaf extract to the culture medium at a concentration of 10 μg/mL to 100 mg/mL. Then, RINm5F cells were dispensed at 5×10 4 into a 96-well plate containing RPMI medium to which 10% FBS and 1% penicillin-streptomycin were added, and cultured to maintain 37° C. and 5% CO 2 . MIN6 cells were added at 37° C. and 5% CO 2 in a 96-well plate containing DMEM medium to which 10% FBS, 1% penicillin-streptomycin, and 1% 55 mM 2-Mercaptoethanol were added. Incubated to maintain. After stabilizing RINm5F cells and MIN6 cells for about 1 day, the prepared extract sample was added at a concentration of 1/100 of 200 μl in a well volume of a 96-well plate. After 4 hours, a concentration of 100 mM STZ (Streptozotocin) was added to RINm5F cells at a concentration of 1/100 of 200 µl of a well volume of a 96-well plate. Then, a concentration of 300 mM STZ (Streptozotocin) was added to MIN6 cells at a concentration of 2 µl, which is 1/100 of a well volume of 200 µl of a 96-well plate. After 24 hours, RINm5F cells and MIN6 cells were added to each well at a concentration of 1/20 of 200 μl of a well volume of a CCK 96-well plate. After reacting in an incubator maintained at 37°C and 5% CO 2 for about 4 hours, absorbance was measured at 540 nm using a plate reader.

2. 관능분석2. Sensory analysis

제조조건에 따른 맛간장의 관능검사는 전문분석기관에 의뢰하여 훈련된 무슬림 패널 39명을 대상으로 7점 척도법으로 실시하였다.The sensory test of taste soy sauce according to the manufacturing conditions was conducted on a 7-point scale for 39 Muslim panelists who were trained by a professional analysis agency.

3. 추출물 및 3. Extract and 맛간장의Soy sauce 혈중 인슐린 증진 효과 검증 Verification of blood insulin enhancement effect

ICR수컷 5주령 마우스에 STZ(streptozotocin) 100 mg/kg을 7일에 한번씩 복강주사하여 당뇨를 유도하였다. 실험 시작과 동시에 마우스 꼬리 정맥에서 혈액을 채취하여 혈당을 측정하였다. 총 14일간 실험을 수행한 후, 마우스 혈청과 췌장을 채취하여 실험 분석에 사용하였다. Diabetes was induced by intraperitoneal injection of 100 mg/kg of streptozotocin (STZ) once every 7 days to 5-week-old ICR male mice. At the same time as the experiment was started, blood was collected from the mouse tail vein and blood glucose was measured. After performing the experiment for a total of 14 days, mouse serum and pancreas were collected and used for experimental analysis.

마우스 혈청 인슐린 농도 측정은 mouse insulin ELISA kit (ALPCO, Salem, MA, USA)을 사용하여 측정하였다. 1차항체가 코팅된 96 웰 플레이트에 standard (6.9 ng/ml, 3.7 ng/ml, 1.25 ng/ml, 0.5 ng/ml, 0.18 ng/ml) 및 혈청 10 ㎕를 로딩하였다. Standard 및 혈청이 로딩된 웰에 반응 conjugate를 각각 75 ㎕씩 로딩한 뒤, 2시간 정도 반응시켰다. 이후 TMB solution 100 ㎕를 로딩하여 발색반응을 유도한 뒤, stop solution 100 ㎕를 넣어주어 반응을 종료시켰다. 인슐린 농도에 따른 발색 정도를 450 nm 파장 아래 흡광도를 측정하여 기록하였다.The mouse serum insulin concentration was measured using a mouse insulin ELISA kit (ALPCO, Salem, MA, USA). Standard (6.9 ng/ml, 3.7 ng/ml, 1.25 ng/ml, 0.5 ng/ml, 0.18 ng/ml) and 10 μl of serum were loaded into a 96-well plate coated with the primary antibody. 75 µl of each reaction conjugate was loaded into the wells loaded with standard and serum, and then reacted for about 2 hours. Thereafter, 100 µl of TMB solution was loaded to induce a color reaction, and then 100 µl of stop solution was added to terminate the reaction. The degree of color development according to the insulin concentration was recorded by measuring the absorbance under the 450 nm wavelength.

실시예Example 1. One. 녹차잎Green tea leaves 물 추출물의 Of water extract 췌장베타세포주Pancreatic beta cell line 사멸보호 효능 Death protection efficacy

췌장베타세포주에 STZ(Streptozotocin) 처리 시 세포사멸로 인해 세포생존에 영향을 나타내는지를 CCK 탐색을 통해 RINm5F 세포와 MIN6 세포에서 확인하였다(도 1). RINm5F 세포에 1000 uM STZ 처리 시, 세포생존율이 약 40%로 감소하였고 STZ와 녹차 물 추출물을 병행 처리하였을 때, 1000 ㎍/ml의 농도에서 세포생존율이 60% 증가함에 따라 세포손상을 억제함을 확인할 수 있었다. MIN6 세포에 3000 uM STZ 처리 시, 세포생존율이 약 60%로 감소하였고 STZ와 녹차 물 추출물을 병행 처리하였을 때, 1000 ㎍/ml의 농도에서 세포생존율이 40% 증가함에 따라 세포손상을 억제함을 확인할 수 있었다. 녹차 물 추출물은 RINm5F 세포와 MIN6 세포에서 STZ 처리를 하지 않은 Blank군과 같은 세포생존율을 보여 항당뇨 효능을 가지는 소재로서 적합하였다.It was confirmed in RINm5F cells and MIN6 cells through CCK screening whether or not the pancreatic beta cell line had an effect on cell survival due to apoptosis when STZ (Streptozotocin) treatment was performed (FIG. 1 ). When 1000 uM STZ treatment on RINm5F cells, the cell viability decreased to about 40%, and when STZ and green tea water extract were treated in parallel, the cell viability increased by 60% at a concentration of 1000 ㎍/ml to suppress cell damage. I could confirm. When MIN6 cells were treated with 3000 uM STZ, the cell viability decreased to about 60%, and when the STZ and green tea water extract were treated in parallel, the cell viability increased by 40% at a concentration of 1000 ㎍/ml to suppress cell damage. I could confirm. Green tea water extract was suitable as a material having antidiabetic efficacy in RINm5F cells and MIN6 cells as it showed the same cell viability as in the blank group without STZ treatment.

실시예Example 2. 2. 조미액Seasoning 및 혼합 추출물의 재료 배합비에 따른 And according to the material mixing ratio of the mixed extract 맛간장의Soy sauce 관능검사 Sensory test

조미액 및 혼합 추출물의 재료 배합비에 따른 맛간장을 이용하여 연두부와 함께 섭취하도록 한 후 관능검사를 실시한 결과는 하기 표 3과 같다.Table 3 shows the results of the sensory test after ingesting it together with the soft tofu using taste soy sauce according to the ingredient mixing ratio of the seasoning liquid and the mixed extract.

조미액 및 혼합 추출물의 재료 배합비에 따른 맛간장의 관능검사Sensory evaluation of taste soy sauce according to the ingredient mixing ratio of seasoning liquid and mixed extract 구분division color incense flavor 전체적 기호도Overall acceptability 제조예 1Manufacturing Example 1 4.024.02 4.304.30 4.644.64 4.504.50 비교예 1Comparative Example 1 3.923.92 3.603.60 3.903.90 3.743.74 비교예 2Comparative Example 2 3.903.90 3.703.70 3.943.94 3.823.82 비교예 3Comparative Example 3 3.883.88 3.843.84 4.184.18 3.903.90

상기 표 3에서 나타낸 바와 같이, 제조예 1의 조미액 및 혼합 추출물을 이용하여 제조한 맛간장이 비교예들의 맛간장에 비해 향, 맛 및 전체적 기호도에서 높은 점수를 나타내어, 본 발명의 재료 종류 및 배합비로 배합한 조미액 및 혼합 추출물을 이용하여 맛간장을 제조하는 것이 기호성에 있어서 상당히 개선되었음을 알 수 있었다.As shown in Table 3, flavored soy sauce prepared using the seasoning liquid and mixed extract of Preparation Example 1 showed a high score in flavor, taste, and overall acceptability compared to the flavored soy sauce of Comparative Examples, and the material type and mixing ratio of the present invention It was found that preparing taste soy sauce using the seasoning liquid and mixed extract was significantly improved in palatability.

실시예Example 3. 재료 배합비에 따른 3. According to the material mixing ratio 맛간장의Soy sauce 관능검사 Sensory test

제조예 1과 비교예 4 및 5의 맛간장을 가지고 연두부와 함께 섭취하도록 한 후 관능검사를 실시한 결과는 하기 표 4와 같다.After the taste soy sauce of Preparation Example 1 and Comparative Examples 4 and 5 was taken together with soft tofu, the sensory test was performed.

재료 배합비에 따른 맛간장의 관능검사Sensory test of taste soy sauce according to the ingredient mixing ratio 구분division color incense flavor 전체적 기호도Overall acceptability 제조예 1Manufacturing Example 1 4.024.02 4.304.30 4.644.64 4.504.50 비교예 4Comparative Example 4 3.783.78 3.483.48 3.603.60 3.543.54 비교예 5Comparative Example 5 3.963.96 3.763.76 3.843.84 3.803.80

상기 표 4에서 나타낸 바와 같이, 비교예 4 및 5의 맛간장에 비해 제조예 1의 맛간장이 더 높은 기호도를 나타내어, 제조예 1의 재료 종류 및 배합비로 맛간장을 제조하는 것이 맛간장의 감칠맛 및 풍미를 더욱 향상시킬 수 있음을 확인할 수 있었다.As shown in Table 4, compared to the taste soy sauce of Comparative Examples 4 and 5, the taste soy sauce of Preparation Example 1 exhibited a higher preference, and thus, the taste of soy sauce was prepared with the material type and mixing ratio of Preparation Example 1. And it was confirmed that the flavor can be further improved.

실시예Example 4. 인슐린 증진 효과 4. Insulin enhancing effect

혼합 추출물과 맛간장을 가지고 인슐린 증진 효과를 비교한 결과는 도 2와 같다. 마우스에 STZ 복강주사로 인하여 혈청 내 인슐린 농도가 유의적으로 감소하였으며, 제조예 1의 맛간장(개발간장) 200 mg/kg 경구투여로 인하여 STZ 단독 투여군 대비 202% 증가하였으며, 양성대조군으로 사용한 치커리 경구투여군 대비 51.06% 높은 효능을 나타내었다(치커리 인슐린 증가율: 134.37%, 개발 맛간장 200 mg/kg 인슐린 증가율: 202.98%).The results of comparing the insulin enhancing effect with the mixed extract and taste soy sauce are shown in FIG. 2. Intraperitoneal injection of STZ in mice significantly reduced the serum insulin concentration, and 202% increased compared to the STZ alone group due to oral administration of 200 mg/kg of taste soy sauce (developed soy sauce) of Preparation Example 1, and chicory used as a positive control. It showed 51.06% higher efficacy compared to the oral administration group (chicory insulin increase rate: 134.37%, developed taste soy sauce 200 mg/kg insulin increase rate: 202.98%).

Claims (5)

(1) 물에 양파껍질, 검정콩, 다시마 및 표고버섯을 첨가하고 끓인 후 여과하여 조미액을 제조하는 단계;
(2) 녹차잎, 바질, 세이보리, 은행잎 및 큐민에 각각 물을 첨가하고 추출한 후 여과하고 농축하여 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 각각 제조하는 단계;
(3) 상기 (2)단계의 제조한 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 혼합하여 혼합 추출물을 제조하는 단계; 및
(4) 맛간장 총 중량 기준으로, 상기 (1)단계의 제조한 조미액, 상기 (3)단계의 제조한 혼합 추출물과 진간장, 어간장, 설탕, 매실청, 사과, 배, 양파, 물엿 및 마늘을 혼합하고 끓인 후 여과하는 단계를 포함하여 제조하는 것을 특징으로 하는 맛간장의 제조방법.
(1) adding onion skin, black beans, kelp and shiitake mushrooms to water, boiling, and filtering to prepare a seasoning solution;
(2) adding water to green tea leaves, basil, savory, ginkgo leaves and cumin, respectively, followed by extraction, filtering, and concentrating to prepare green tea extract, basil extract, savory extract, ginkgo leaf extract, and cumin extract, respectively;
(3) preparing a mixed extract by mixing the green tea extract, basil extract, savory extract, ginkgo leaf extract and cumin extract prepared in step (2); And
(4) Based on the total weight of taste soy sauce, the seasoning liquid prepared in step (1), the mixed extract prepared in step (3), and soy sauce, fish soy sauce, sugar, plum green, apple, pear, onion, starch syrup, and garlic Mixing, boiling, and filtering the method for producing flavored soy sauce, characterized in that it comprises the step of filtration.
제1항에 있어서, 상기 (4)단계의 혼합은 맛간장 총 중량 기준으로, 조미액 16~17 중량%, 혼합 추출물 19~21 중량%, 진간장 23~26 중량%, 어간장 1.8~2.4 중량%, 설탕 3~4 중량%, 매실청 1.8~2.4 중량%, 사과 9~10 중량%, 배 9~10 중량%, 양파 9~10 중량%, 물엿 3~4 중량% 및 마늘 0.1~0.3 중량%를 혼합하는 것을 특징으로 하는 맛간장의 제조방법.The method of claim 1, wherein the mixing in step (4) is based on the total weight of taste soy sauce, seasoning liquid 16 to 17 wt%, mixed extract 19 to 21 wt%, soy sauce 23 to 26 wt%, fish soy sauce 1.8 to 2.4 wt%, Sugar 3-4 wt%, plum green 1.8-2.4 wt%, apple 9-10 wt%, pear 9-10 wt%, onion 9-10 wt%, starch syrup 3-4 wt%, and garlic 0.1-0.3 wt% A method for producing taste soy sauce, characterized in that mixing. 제2항에 있어서,
(1) 물 80~120 mL에 양파껍질 0.05~0.07 g, 검정콩 1.8~2.2 g, 다시마 0.08~0.12 g 및 표고버섯 0.06~0.08 g을 첨가하고 끓인 후 여과하여 조미액을 제조하는 단계;
(2) 녹차잎, 바질, 세이보리, 은행잎 및 큐민에 각각 물을 8~12배(v/w) 첨가하여 추출한 후 여과하고 농축하여 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 각각 제조하는 단계;
(3) 상기 (2)단계의 제조한 녹차 추출물 18~22 mL, 바질 추출물 18~22 mL, 세이보리 추출물 8~12 mL, 은행잎 추출물 8~12 mL 및 큐민 추출물 8~12 mL를 혼합하여 혼합 추출물을 제조하는 단계; 및
(4) 맛간장 총 중량 기준으로, 상기 (1)단계의 제조한 조미액 16~17 중량%, 상기 (3)단계의 제조한 혼합 추출물 19~21 중량%와 진간장 23~26 중량%, 어간장 1.8~2.4 중량%, 설탕 3~4 중량%, 매실청 1.8~2.4 중량%, 사과 9~10 중량%, 배 9~10 중량%, 양파 9~10 중량%, 물엿 3~4 중량% 및 마늘 0.1~0.3 중량%를 혼합하고 끓인 후 여과하는 단계를 포함하여 제조하는 것을 특징으로 하는 맛간장의 제조방법.
The method of claim 2,
(1) adding 0.05 to 0.07 g of onion skin, 1.8 to 2.2 g of black beans, 0.08 to 0.12 g of kelp, and 0.06 to 0.08 g of shiitake mushrooms to 80 to 120 mL of water, boiling, and filtering to prepare a seasoning solution;
(2) Add 8-12 times (v/w) of water to green tea leaves, basil, savory, ginkgo leaves and cumin, extract, filter, and concentrate to extract green tea extract, basil extract, savory extract, ginkgo leaf extract and cumin extract, respectively. Manufacturing steps;
(3) Mixed extract by mixing 18 to 22 mL of green tea extract prepared in step (2), 18 to 22 mL of basil extract, 8 to 12 mL of savory extract, 8 to 12 mL of ginkgo leaf extract, and 8 to 12 mL of cumin extract Manufacturing a; And
(4) Based on the total weight of taste soy sauce, 16 to 17% by weight of seasoning liquid prepared in step (1), 19 to 21% by weight of mixed extract prepared in step (3) and 23 to 26% by weight of soy sauce, fish soy sauce 1.8 ~2.4 wt%, sugar 3~4 wt%, plum green 1.8~2.4 wt%, apple 9-10 wt%, pear 9-10 wt%, onion 9-10 wt%, starch syrup 3-4 wt%, and garlic 0.1 Mixing ~0.3% by weight, boiling and filtering the method for producing taste soy sauce, characterized in that it comprises the step of.
제3항에 있어서,
(1) 물 80~120 mL에 양파껍질 0.05~0.07 g, 검정콩 1.8~2.2 g, 다시마 0.08~0.12 g 및 표고버섯 0.06~0.08 g을 첨가한 후 90~100℃에서 8~12분 동안 끓인 후 여과하여 조미액을 제조하는 단계;
(2) 녹차잎, 바질, 세이보리, 은행잎 및 큐민에 각각 물을 8~12배(v/w) 첨가한 후 55~65℃에서 4~6시간 동안 추출한 후 여과하고 75~85℃에서 농축하여 녹차 추출물, 바질 추출물, 세이보리 추출물, 은행잎 추출물 및 큐민 추출물을 각각 제조하는 단계;
(3) 상기 (2)단계의 제조한 녹차 추출물 18~22 mL, 바질 추출물 18~22 mL, 세이보리 추출물 8~12 mL, 은행잎 추출물 8~12 mL 및 큐민 추출물 8~12 mL를 혼합하여 혼합 추출물을 제조하는 단계; 및
(4) 맛간장 총 중량 기준으로, 상기 (1)단계의 제조한 조미액 16~17 중량%, 상기 (3)단계의 제조한 혼합 추출물 19~21 중량%와 진간장 23~26 중량%, 어간장 1.8~2.4 중량%, 설탕 3~4 중량%, 매실청 1.8~2.4 중량%, 사과 9~10 중량%, 배 9~10 중량%, 양파 9~10 중량%, 물엿 3~4 중량% 및 마늘 0.1~0.3 중량%를 혼합하고 90~100℃에서 4~6분 동안 끓인 후 여과하는 단계를 포함하여 제조하는 것을 특징으로 하는 맛간장의 제조방법.
The method of claim 3,
(1) After adding 0.05 to 0.07 g of onion skin, 1.8 to 2.2 g of black beans, 0.08 to 0.12 g of kelp, and 0.06 to 0.08 g of shiitake mushrooms in 80 to 120 mL of water, boil at 90 to 100°C for 8 to 12 minutes. Filtering to prepare a seasoning liquid;
(2) Add 8 to 12 times (v/w) water to green tea leaves, basil, savory, ginkgo leaves and cumin, respectively, extract at 55 to 65°C for 4 to 6 hours, filter, and concentrate at 75 to 85°C. Preparing green tea extract, basil extract, savory extract, ginkgo leaf extract, and cumin extract, respectively;
(3) Mixed extract by mixing 18 to 22 mL of green tea extract prepared in step (2), 18 to 22 mL of basil extract, 8 to 12 mL of savory extract, 8 to 12 mL of ginkgo leaf extract, and 8 to 12 mL of cumin extract Manufacturing a; And
(4) Based on the total weight of taste soy sauce, 16 to 17% by weight of seasoning liquid prepared in step (1), 19 to 21% by weight of mixed extract prepared in step (3) and 23 to 26% by weight of soy sauce, fish soy sauce 1.8 ~2.4 wt%, sugar 3~4 wt%, plum green 1.8~2.4 wt%, apple 9-10 wt%, pear 9-10 wt%, onion 9-10 wt%, starch syrup 3-4 wt% and garlic 0.1 Mixing ~0.3% by weight, boiling for 4-6 minutes at 90 ~ 100 ℃, and then filtering the method for producing flavored soy sauce.
제1항 내지 제4항 중 어느 한 항의 방법으로 제조된 맛간장.Flavored soy sauce prepared by the method of any one of claims 1 to 4.
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