KR20200115370A - Method for capturing fine material using condensation and relaxation of biopolymer molecular structure - Google Patents

Abstract

The present invention relates to a method for capturing a fine material using condensation and relaxation of a biopolymer molecular structure. Specifically, by using a biopolymer in which the condensation degree or relaxation degree of a molecular structure changes according to an environmental change, a fine material comes into contact with a biopolymer in which a molecular structure is relaxed, and the molecular structure of the biopolymer is condensed, such that the fine material is mixed with the biopolymer. When the method for capturing a fine material or a composition for capturing a fine material of the present invention is used, a fine material, specifically, fine dust can be effectively collected. In particular, when skin is applied to a human body, fine dust can be effectively prevented from being deposited to skin, or pre-deposited fine dust can be effectively removed. A collection effect with respect to microorganisms is also excellent, and thus the present invention can also be applied to health hygiene. In addition, when the method for producing a fine material captured structure of the present invention is used, a captured structure having an excellent capturing effect or suitable to be used as an active ingredient of a fine material collecting composition or to apply the fine material collecting method of the present invention can be produced.

Description

Method for capturing fine material using condensation and relaxation of biopolymer molecular structure

The present invention relates to a method of collecting microscopic substances using condensation and relaxation of a biopolymer molecular structure, and specifically, a living body having a relaxed molecular structure by using a biopolymer whose degree of condensation or relaxation of the molecular structure varies according to changes in the environment. The present invention relates to a method for collecting micromaterials, characterized in that the micromaterials are woven into the biopolymer by contacting a micromaterial with a polymer and condensing the molecular structure of the biopolymer, and a technology using the same.

Recently, the concentration of fine matter, that is, fine dust in the atmosphere, has increased, threatening the health of modern people. Accordingly, there is a demand for the development of technology to reduce the damage caused by fine dust.

Dust refers to particulate matter that floats or scatters in the air. It is often generated when burning fossil fuels such as coal and petroleum, or from exhaust gases such as factories and automobiles. Dust is classified into total dust (TSP, Total Suspended Particles), which is less than 50㎛, and fine dust (PM, Particulate Matter), which has a very small particle size, depending on the size of the particles. Fine dust is further divided into fine dust (PM10) whose diameter is smaller than 10㎛ and fine dust (PM2.5) whose diameter is smaller than 2.5㎛. Ultrafine dust is expressed as PM0.1 and nano dust is expressed as PM0.05. In general, PM2.5 is 50 to 70% of PM10 amount.

The components of fine dust have been researched and investigated as soot, sulfate, nitrate, metals, and organic matter. These substances alone form dust, but most of them combine with each other to have a composite structure (see Fig. 1).

Fine dust enters and deposits through the skin, eyes and respiratory tract, or is distributed throughout the body through blood. Body organs where fine dust is mainly accumulated are skin, eyes, tissue cell surfaces, cell membranes or cytoplasm, and fat layers (see FIG. 2).

Fine dust has an adverse effect on the environment, but when the body is deposited or penetrated into the body, the effects on human health include early death due to heart and lung disease, non-fatal heart attack, irregular heartbeat, worsening asthma, decreased lung function, and coughing and breathing. It has been reported as respiratory symptoms such as difficulty.

Looking at the way fine dust penetrates into the skin, it penetrates into the body through hair follicles, skin wounds, or bronchi, causing inflammation (see FIG. 3).

On the other hand, in living tissues, the expression of inflammation is more severe for fine dust, especially for fat-soluble PM10, than for PM2.5, and there is inflammation in the ultrafine dust, but the degree of it is being studied. In addition, the action against the human body's fine dust promotes the generation of lymphocytes through an immune response, thereby releasing the infiltrating fine dust.

In order to prevent serious problems caused by the penetration of fine dust into the body, a technology that blocks fine dust before contacting the body, for example, a technology that collects fine dust in the air, is also important, but fine dust that has already contacted the body It is also very important to remove particles or prevent fine dust from penetrating into the body from the surface of the body. And for this purpose, it must be able to be applied safely to the human body in particular, and it is necessary to increase its effectiveness as it can be applied to everyday life products.

Platinum Metals Rev., 2009, 53, (1), 27. Biomed Res Int. 2013;2013:279371. Journal of Dermatological Science, Volume 91, Issue 2, August 2018, Pages 175-183.

The main object of the present invention is to effectively collect fine substances, particularly fine dust, by using a substance derived from a living body, so that when applied to the skin of the human body, it is possible to prevent deposits of fine substances or to easily remove fine substances. It is to provide a method of collecting fine substances.

Another object of the present invention is to provide a composition for collecting fine substances for applying the method for collecting fine substances.

Another object of the present invention is to provide a method for preparing a micromaterial collector for applying the method for collecting micromaterials or for use as an active ingredient in the composition for collecting micromaterials.

According to one aspect of the present invention, the present invention uses a biopolymer whose degree of condensation or relaxation of the molecular structure varies according to changes in the environment, and contacts a micromaterial with a biopolymer having a relaxed molecular structure. It provides a method for collecting micromaterials, characterized in that the micromaterial is woven into the biopolymer by condensing the structure.

In the method for collecting microscopic substances of the present invention, it is preferable that the biopolymer is beta glucan, and the relaxation and condensation of the molecular structure is caused by a change in pH.

According to another aspect of the present invention, the present invention contains as an active ingredient a biopolymer whose degree of condensation or relaxation of the molecular structure varies according to changes in the environment, and forms an environment in which the molecular structure of the biopolymer is relaxed. It provides a composition for collecting fine substances.

In the composition for collecting microscopic substances of the present invention, the biopolymer is beta-glucan, and the environment in which the molecular structure is relaxed is preferably an acidic environment having a pH of 5 or less.

In the composition for collecting fine matter of the present invention, it is preferable that the composition is used for preventing or removing the deposition of fine dust by collecting fine dust on the body surface.

In the composition for collecting fine substances of the present invention, the composition is preferably a cream, lotion, foundation, soap, shampoo, toothpaste, gargle, or a mask pack formulation.

According to another aspect of the present invention, the present invention extracts the biopolymer from a biological material containing a biopolymer whose degree of condensation or relaxation of the molecular structure varies according to changes in the environment, wherein the molecular structure of the biopolymer is relaxed. It provides a method for producing a micromaterial collector, characterized in that the biopolymer is separated from other components of the biological material and obtained by creating an environment to be obtained.

In the method for producing a microscopic substance collector of the present invention, it is preferable that the biopolymer is beta glucan, the biological material is mushroom, and the environment in which the molecular structure is relaxed is an acidic environment of pH 5 or less.

When the method for collecting fine substances or the composition for collecting fine substances of the present invention is used, fine substances, particularly fine dust, can be effectively collected. In particular, when applied to the skin of the human body, it can effectively prevent deposits of fine dust on the skin or effectively remove already deposited fine dust, and has an advantage that can be applied to health and hygiene as it has excellent trapping effects on microorganisms. . In addition, the use of the method for producing a fine substance collector of the present invention can produce a collector having excellent collecting effect suitable for use as an active ingredient in a composition for collecting fine substances or applying the method for collecting fine substances of the present invention. .

1 shows the configuration of fine dust (particularly fine particles discharged from automobiles).
Figure 2 shows the size of the fine dust penetrating due to breathing and the target body organs.
3 shows a method of penetration of fine dust.
4 shows the helix structure and superhelix structure of a biopolymer having a helix structure such as protein, nucleic acid, and starch.
Figure 5 shows the conversion between the super-helix structure and the general helix structure of the biopolymer.
6 shows a process in which a biopolymer having a superhelix structure present in a state of being combined with another material is disassembled into a coil-type single molecule or, conversely, is converted from a coil-type single molecule to a superhelix structure.
7 shows how fine dust is woven into the biopolymer.
8 shows the principle and application range of the biopolymer (betaglucan) weaving micromaterials according to the present invention.
9 shows a comparison of the extraction results according to the difference in �™� diameter of the pH during the process of extracting beta glucan using chaga according to an embodiment of the present invention.
10 is a SEM (Scanning Electron Microscopy) photograph of a biopolymer (betaglucan) induced to form a superhelix structure according to an embodiment of the present invention.
11 is a result of an experiment in which water-soluble anthocyanins are collected with a biopolymer (betaglucan) according to an embodiment of the present invention.
12 is a result of an experiment in which a fat-soluble marigold extract is collected with a biopolymer (betaglucan) according to an embodiment of the present invention.
13 is a result of an experiment in which lactic acid bacteria having a size of about 1 μm are collected with a biopolymer (betaglucan) according to an embodiment of the present invention.
14 is a result of an experiment in which fine dust or lactic acid bacteria are collected with a biopolymer (betaglucan) according to an embodiment of the present invention.
FIG. 15 is a result of testing the effect of a biopolymer (betaglucan) for preventing fine dust skin deposition according to an embodiment of the present invention.
16 is a result of an experiment on the effect of removing fine skin dust of a biopolymer (betaglucan) according to an embodiment of the present invention.

The helix structure is a screw-shaped structure that biopolymers such as proteins, nucleic acids, and starches take, and the super-helix structure is a helix structure in which several unit helix structures are formed again in several layers (see FIG. 4).

The helix structure, a structure famous for the double helix of DNA, is partially present in protein molecules, and in chitosan processed from proteoglycan and crustacean chitin, it is found in multi-layered helix state, grains, bacteria, yeast, and mushroom Beta glucan, which is a component of animal hair, keratin, and collagen found in various tissues of animals exist in triple helix helix structure and super helix structure. Proteoglycan and chitosan have a helix structure, but they become macromolecules due to intermolecular aggregation. Unlike this, DNA, beta-glucan, keratin, and collagen form super-helix through hydrogen bonds between molecules and between helixes.

The characteristics of the super-helix structure of a biopolymer discovered by the present inventors are that the super-helix structure is disassembled in an alkaline state for keratin and in an acidic state for beta-glucan and collagen to form a coiled monomolecule. It develops into a multi-layered super-helix structure (see Fig. 5). And proteoglycan and chitosan aggregate in an acidic state and dissociate in an alkaline state.

In the natural state, super-helix and agglomerates that exist in a woven state of other substances become single-helix structures and coil-type monomolecules under certain conditions, releasing the woven material, and when they become helix or super-helix structures and aggregates, Another material is weaved (see Fig. 6).

By disassembling the super-helix structure in which other materials are woven by using the characteristics of this structure transformation, the single helix or coil-type single molecule without the woven material approaches fine dust and forms a super-helix structure. Can weave. Using this principle, fine dust that approaches the skin or respiratory tract can be woven into the super helix to prevent the deposition of fine dust, and even the deposited fine dust can be woven into the super helix and wiped off to remove the deposited fine dust. .

In connection with the application of the present invention, particularly the pH in the path of fine dust is an important issue. In the human body, the nasal cavity and bronchi have homeostasis that always maintains a pH of 7.4, which is a weak alkali, and the skin maintains homeostasis with a weak acidity of pH 5.5. The nasal cavity and bronchi maintain pH 7.4 by controlling the amount of body fluid containing carbonates, and the skin maintains pH 5.5 homeostasis with salts of sweat and water, and fatty acids of sebum. In addition, the nasal and bronchial nose hairs and cilia protect the nasal cavity and bronchi by filtering adsorption or discharge of external impurities.

By helping the body's defense ability, the conversion of the helix structure according to the pH of biopolymers including beta-glucans, especially when converting to a super-helix structure, becomes a complex helix by tying the surrounding substances, and furthermore, it becomes a larger super-helix. It can be effectively used to prevent direct deposition or to remove deposited fine dust.

Looking at the method of combining the fine dust and the biopolymer according to the present invention, when the helix is formed, the fine dust is trapped to form a combination, and the combination is entangled with each other to form a larger structure (see FIG. 7).

In order to maximize the efficiency of these properties, biopolymers including beta-glucan must be purified in a state that is not woven with foreign substances. This is because a biopolymer in a state that is highly interwoven with other materials has a poor ability to bind other materials by itself. Therefore, a method of manufacturing a biopolymer material in a coiled or single-helix state is also very important.

Looking at the representative biopolymers as described above, including beta glucan, are as follows.

Beta-glucan is a material that firmly maintains the outer skin of cereals or the cell membrane of mushrooms, and is a polysaccharide polymer having a molecular weight of 150,000 to 500,000 Da in which monosaccharides have beta 1-3 bonds as the main axis and beta 1-6 bonds in the middle. In reality, proteins and other types of low-molecular substances are woven together in a living body.

Collagen is one of the hard proteins of approximately 300,000 Da and is present in bones and skin as a main component of connective tissues of animals. There are several types of biopolymers that have a triple helix structure.

Chondroitin sulfate is a kind of sulfated viscous protein that forms cartilage, and its basic units are N-acetylgalactosamine and glucuronic acid, and its average molecular weight is about 15000 to 30,000 Da, which has a similar structure. Like proteins, they exist in a double, quad, or eight-fold helix structure.

Keratin exists in body hair such as hair, animal horns, and nails, toenails, and such keratin forms a physically hard structure by disulfide bonds. Human keratin has a molecular weight of approximately 44000 to 66,000 Da and has a triple helix structure.

Chitin or crustacean (甲殼素) is a polymer polysaccharide in which N-acetylglucosamine is bound in a long chain form. It is an important component of the hard epidermis of arthropods, the shells of mollusks, and the cell walls of fungi. Plant cells have a cell wall called cellulose, while the cell wall of fungi contains a hard component of chitin contained in shells such as crabs and shrimp. Chitosan is called chitosan, which is relatively low molecular weight by treating chitin with an alkali, etc., and the average molecular weight of chitosan is 3800 ~ 20,000 Da. Chitosan also has a helix structure.

These biopolymers exist as a super-helix structure in which other materials are interwoven, and thus maintain firmness and elasticity in cells.

Representatively, explaining through the experimental results of beta-glucan, it is twisted from a random coil structure in an acidic solvent to a helix structure in an alkaline solution, and transformed into a superhelix structure in a stronger alkali. And when there is a low molecular weight substance in the solution, when it is converted to a helix structure, the low molecular weight substance is interposed in the structure to form an initial primary conjugate. After that, as the structure conversion develops, it becomes a super-helix structure, and it becomes a complex in which more and more low-molecular substances are stuck.

Another of the characteristics of the structural change of beta-glucan is that the structure changes in organic solvents such as ethanol, methanol, and acetone, rather than in aqueous solutions, and changes into a superhelix structure by inserting a low-molecular substance. At this time, when the concentration of the organic solvent is gradually increased, a superhelix structure is gradually formed. At this time, the amount of the material inserted is approximately the same weight as that of beta-glucan. In other words, it becomes a high-concentration material complex.

By utilizing these characteristics, not only water-soluble fine dust but also poorly soluble fine dust can be inserted into the helix structure.

Until now, methods of removing fine dust have been introduced to reduce the amount of fine dust discharged by dust collection, filtration, and electrochemical methods in terms of environmental protection, but the filtration method using a mask is the main technology in terms of health protection.

As a technique similar to the present invention, there are cosmetics containing viscous natural extracts and additives with anti-inflammatory action. However, this method prevents skin deposition of fine dust by applying a viscous substance to the skin and prevents inflammation with an anti-inflammatory agent, which is quite different from the present invention.

The technology of the present invention is unique in that it cannot pass through the skin layer physicochemically by inserting it into a biopolymer helix structure such as beta glucan, so that it cannot pass through the skin layer physicochemically by inserting it into a biopolymer helix structure such as beta glucan. .

The technology of the present invention can form a super-helix by combining fine dust and ultra-fine dust deep in the skin with a coil type or single helix having an average size of about 150 to 170 nanometers, making it easy to remove already deposited fine dust. do.

When explaining the characteristics of the superhelix structure discovered by the present inventors, the superhelix of keratin and viscous proteins is in an alkaline state, and the superhelix of beta-glucan, collagen, chitosan, etc. is dissolved in the acidic state, resulting in a coiled single molecule. Becomes. In addition, beta-glucan is extracted in the form of a random coil in which the woven material is minimized in acidic conditions, and in the form of a complex containing the woven material in alkaline conditions. The interwoven material can be changed to a minimized random coil shape. In addition, when beta-glucan is changed from a random coil structure to a helix structure, it ties the surrounding materials and then becomes a super-helix structure. In the super-helix structure, when the concentration of an organic solvent is increased or dried, the super-helix structure becomes larger and more surrounding materials can be woven under the conditions under which the alkali condition or water activity decreases. In acidic conditions, this superhelix structure can unravel and release the interwoven material. By using this property, it is possible to insert fine dust into the beta glucan, so that it is possible to prevent deposition and remove the deposited fine dust. In addition, it can be used to clean waste products, toxins, and germs by using beta-glucan in a random coil state.

Based on the above principle, the present invention uses a biopolymer whose degree of condensation or relaxation of the molecular structure varies according to changes in the environment, and contacts a micromaterial with a biopolymer having a relaxed molecular structure. It provides a method for collecting micromaterials, characterized in that the micromaterial is woven into the biopolymer by condensing the structure.

In the method for collecting micromaterials of the present invention, the biopolymer may be, for example, beta glucan, collagen, chondroitin sulfate, keratin, chitin, or any combination thereof, preferably beta glucan.

Relaxation or condensation of the molecular structure in the method for collecting microscopic substances of the present invention may be performed according to environmental changes such as pH, temperature, water activity, etc., and preferably, according to the change of pH. For example, when the biopolymer is beta-glucan, the molecular structure can be made a relaxed state by lowering the pH of the solution containing beta-glucan (for example, pH 5 or less) to make the molecular structure relaxed. Alternatively, alkalinization can lead to a condensed molecular structure.

The condensation in the method of collecting micro-materials of the present invention should be understood as a relative concept for relaxation. For example, from a helix structure to a superhelix structure, from a single helix structure to a double or triple or more multiple helix structure, from a monomer to a dimer or a higher polymer (such as a trimer ), tetramers, etc.] may correspond to condensation.

In the method for collecting fine substances of the present invention, the fine substances include compounds such as fine dust and pigments, microorganisms, viruses, etc., preferably fine dust, and more preferably fine dust having an average diameter of 10 μm or less. , More preferably, the particles have an average diameter of 2.5 µm or less, 0.1 µm or less, or 0.05 µm or less.

The present invention is also a composition for applying the method of collecting micromaterials, containing a biopolymer whose degree of condensation or relaxation of the molecular structure varies according to changes in the environment as an active ingredient, wherein the molecular structure of the biopolymer is relaxed. It provides a composition for collecting micro-materials, characterized in that forming a.

In the composition for collecting micro-materials of the present invention, matters related to biopolymers, relaxation or condensation of molecular structures, micro-materials, etc. may be applied in the same manner as suggested in the method for collecting micro-materials.

The environment in which the molecular structure is relaxed in the composition for collecting micromaterials of the present invention may be an acidic environment in which the pH of a solution containing beta-glucan is 5 or less, 4 or less, 3 or less, or 2 or less, for example, when the biopolymer is beta glucan. have.

The composition for collecting fine substances of the present invention, in particular, the biopolymer is beta-glucan, and the composition is formed in an acidic environment under the above pH condition, especially for use in preventing or removing fine dust deposits by collecting fine dust on the body surface. If used, more effective application is possible. In fact, the present inventors collect the fine dust when the fine dust comes into contact after the composition of the present invention is applied to the skin so that the fine dust is not deposited on the skin, and the composition of the present invention is applied to the skin after the fine dust comes into contact with the skin. If so, it was confirmed that the fine dust existing between the skin surface as well as the skin gap was collected so that it could be washed easily. Therefore, when the composition of the present invention is formulated and applied in a form that is maintained in contact with the skin, such as a cream, lotion, foundation, etc., it can effectively prevent the deposition of fine dust by collecting fine dust in contact with the skin. When it is formulated and applied in a form that is removed after contact with the skin such as soap, shampoo, toothpaste, gargle, mask pack, etc., it can effectively remove fine dust deposited on the skin. The composition for collecting fine substances of the present invention is preferably a cosmetic composition.

The present invention is also a method for preparing a collector for applying the method for collecting micromaterials or for use as an active ingredient in the composition for collecting micromaterials, wherein the degree of condensation or relaxation of the molecular structure varies depending on the environment. Extracting the biopolymer from a biological material containing a biopolymer, creating an environment in which the molecular structure of the biopolymer is relaxed, and collecting the biopolymer by separating the biopolymer from other components of the biological material. Provides a method of making a sieve.

In the method of manufacturing a micromaterial collector of the present invention, matters related to the biopolymer, the environment in which the molecular structure is relaxed, micromaterials, etc. may be applied in the same manner as suggested in the method for collecting micromaterials or the composition for collecting micromaterials.

Therefore, in the method for producing a micromaterial collector of the present invention, the biological material may be a biological material containing the biopolymer, that is, beta glucan, collagen, chondroitin sulfate, keratin, chitin, or any combination thereof. For example, the biological material may be the outer skin of cereals or mushrooms containing beta-glucan, may be bones or skin of animals containing collagen, may be cartilage of animals containing chondroitin sulfate, and keratin It may be the body hair, horns, nails or toenails of the containing animal, and may be the hard epidermis of an arthropod containing chitin, the shell of a mollusk, or the cell wall of a fungus. It is preferably a mushroom.

In the method of manufacturing a micromaterial collector of the present invention, an existing conventional extraction method according to the type of biological material may be used for the extraction of the biopolymer. For example, a method of extracting beta-glucan from mushrooms using a solvent may be used, but may be a method of extracting by mixing mushrooms and a solvent and adjusting the pH to 5 or less, or a conventional extraction method According to the method of extracting beta-glucan, adding a solvent to the extracted beta-glucan, adjusting the pH to 5 or less, and separating other components bound to the beta-glucan may be added. The existing technology for separating beta glucans was introduced by, for example, Wenyan Leong et al. ( Coloids and Surfaces B: Biointerfaces , Volume 146, 1 October 2016, Pages 334-342).

In the method for producing a micromaterial collector of the present invention, the separation, that is, separating the biopolymer from other components of the biological material is by using a conventional separation method using differences in specific gravity, size, solubility, etc. of the material, for example It may be one using filtration or centrifugation.

Hereinafter, the present invention will be described in more detail through examples. Since these examples are for illustrative purposes only, the scope of the present invention is not to be construed as being limited by these examples.

[Example]

1. Beta glucan extraction

1) For fresh or dried mushrooms, add purified water 20 times the weight of fresh mushrooms, and 30 times the weight of dry mushrooms.

2) After adjusting the pH to 1 to 5 with hydrochloric acid for food additives or organic acid, etc., pulverize with a homogeneous mixer.

3) Extract by stirring or circulating for 2 to 5 hours at 40 to 90°C. It is filtered or centrifuged to take a supernatant.

4) Concentrate the supernatant in vacuum to adjust the solid content to about 5%, then filter or centrifuge again to take the supernatant.

5) Here, 95% alcohol is added 2 to 4 times as compared to the supernatant to centrifuge the beta-glucan with minimized binding and recover as a precipitate.

6) A small amount of purified water and edible hydrochloric acid or organic acid are used as a mushroom beta-glucan solution with a pH of 3 to 5 with minimal binding.

In the case of producing purified beta-glucan with a further minimized woven state, the processes 5) and 6) are repeated.

2. Investigation of differences according to pH during beta glucan extraction

After adding 200 ml of water to 5 g of chaga mushrooms, the solution was adjusted to pH 10.5 with caustic soda or caustic garri, and then boiled for 2 hours. Thereafter, the solids were removed by filtration and centrifugation to obtain 150 ml of a dark brown extract (see Fig. 9A). Of these, 5 ml of each was taken, 10 ml of purified water was added, one was adjusted to pH 3.0 with hydrochloric acid, and the other was adjusted to pH 10.5. As a result of this, as shown in B of FIG. 9, blackish brown turned to light brown at liquid pH 3.0, and blackish brown at liquid pH 10.5.

These were transferred to a 50 ml tube, 30 ml of 95% alcohol was added, mixed well, and centrifuged for 5 minutes at 8000 rpm in a high-speed centrifuge. As shown in C of FIG. 9, a small amount of yellowish brown precipitate was generated in the acidic liquid phase, and dark blackish brown precipitate was generated in the alkaline liquid phase.

This supports that beta-glucan extracted in a woven state in an alkaline liquid releases the woven material in an acidic state.

3. Beta-glucan superhelix structure

A 1% beta-glucan aqueous solution (pH 5.0) was prepared, and NaOH solution was added thereto to make pH 7.4, and then left to stand for 10 hours to create a beta-glucan superhelix structure. This was photographed by SEM (Scanning Electron Microscopy) (see FIG. 10).

In the upper left of FIG. 10, a small beta-glucan superhelix structure and a larger beta-glucan superhelix structure in the middle can be seen.

4. Anthocyanin capture

Anthocyanin solution (Brix 1%) extracted with water from blueberry fruit was prepared, divided into two, purified water in one (a) and beta glucan in the other (b) to make 5%, then a was concentrated, and b was The precipitated beta-glucan and the woven material were obtained by centrifugation, dried on a glass plate, and then confirmed by SEM (see FIG. 11).

In Figure 11, the anthocyanin concentrate dried product (a) and the beta-glucan conjugate were dried in distinctly different forms. The beta-glucan conjugate formed a large and small super-helix structure by weaving the blueberry concentrate (b).

5. Capture of fat-soluble marigold extract

The marigold pigment was extracted with olive oil, and the oil layer in which the pigment was dissolved was recovered, suspended in purified water, and divided into two. A certain purified water was added to one side and beta glucan was added to the other to obtain a 5% beta glucan solution, and both sides were made to have the same volume. After leaving for 5 hours, the properties of the product were observed, and the product was recovered, dried on a glass plate, and observed by SEM (see FIG. 12).

As shown in the left test tube of FIG. 12 (a), the marigold olive oil extract was attached to the wall of the test tube in the form of oil in the water layer, and as in the right test tube, the product woven with beta glucan was in a suspended state.

When observed by SEM, the marigold olive oil extract was in a dry state as it was in the SEM (b), but the beta-glucan and the products of the marigold olive oil extract were in a woven state (c). If you look at the enlarged photo (d), you can see that it is woven with beta-glucan helix.

6. Lactobacillus collection

Lactic acid bacteria are approximately 1㎛ in size. Lactobacillus was cultured and the binding form with beta-glucan was observed by SEM (see FIG. 13).

In FIG. 13, it can be seen that the lactic acid bacteria are also woven into beta glucan to form a super-helix structure (a) as in the photograph (c) taken at high magnification.

7. Collection of fine dust

The diesel vehicle was started and fine dust from the exhaust tank was attached to a vacuum filter using a membrane filter to collect fine dust. This was mixed with beta glucan and the conditions were compared under a microscope. Fine dust was woven into beta-glucan to form a complex (see FIG. 14).

8. Prevention of fine dust deposition

8-1. Preparation of fine dust solution

0.1 g of fine dust collected by the method of 7 was dissolved in 10 ml of 95% alcohol and stored in a sealed manner.

8-2. Experiment to confirm the prevention of fine dust deposition

Using a pipette and a spatula, 1 ml of the beta-glucan solution obtained by the above method was evenly applied to the human skin of the experimental group, and the same amount of water was applied to the skin of the control group.

After drying under blowing, 0.2 ml of a fine dust solution was evenly applied, dried by blowing again, washed with water, and observed under a microscope.

As a result, in the control group, a large amount of fine dust was observed on the skin surface, but in the experimental group, fine dust was not observed on the skin surface (see FIG. 15).

9. Deposited fine dust removal experiment

0.2 ml of the fine dust solution of 8-1 was evenly applied to human skin and dried for 5 hours. Thereafter, the experimental group was subjected to the beta-glucan solution obtained by the method of 1 above, and the control group was observed with a microscope before and after washing after rolling water with a finger for 60 seconds.

As a result, a considerable amount of fine dust remained in the control group, but almost all fine dust was removed in the experimental group (see FIG. 16).

Claims (8)

Using a biopolymer whose degree of condensation or relaxation of the molecular structure varies according to changes in the environment, a micromaterial is brought into contact with the biopolymer having a relaxed molecular structure, and the molecular structure of the biopolymer is condensed, so that the micromaterial is transferred to the biopolymer. A method of collecting fine substances, characterized in that it is woven into a polymer. The method of claim 1,
The biopolymer is beta glucan,
The method of collecting fine substances, characterized in that the relaxation and condensation of the molecular structure is caused by a change in pH. A composition for collecting micromaterials, characterized in that it contains as an active ingredient a biopolymer whose degree of condensation or relaxation degree of a molecular structure varies according to changes in environment, but forms an environment in which the molecular structure of the biopolymer is relaxed. The method of claim 3,
The biopolymer is beta glucan,
The composition, characterized in that the environment in which the molecular structure is relaxed is an acidic environment of pH 5 or less. The method of claim 4,
The composition is a composition, characterized in that the use for preventing or removing the deposition of fine dust by collecting the fine dust on the body surface. The method of claim 5,
The composition is a composition characterized in that the formulation of a cream, lotion, foundation, soap, shampoo, toothpaste, gargle or mask pack. Extracting the biopolymer from a biological material containing a biopolymer whose degree of condensation or relaxation of the molecular structure varies according to changes in the environment, creating an environment in which the molecular structure of the biopolymer is relaxed, and from other components of the biological material A method for producing a micromaterial collector, characterized in that obtained by separating the biopolymer. The method of claim 7,
The biopolymer is beta glucan,
The biological material is a mushroom,
The environment in which the molecular structure is relaxed is an acidic environment having a pH of 5 or less.

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