KR20200068181A - a manganese detecting method - Google Patents
a manganese detecting method Download PDFInfo
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- KR20200068181A KR20200068181A KR1020180154846A KR20180154846A KR20200068181A KR 20200068181 A KR20200068181 A KR 20200068181A KR 1020180154846 A KR1020180154846 A KR 1020180154846A KR 20180154846 A KR20180154846 A KR 20180154846A KR 20200068181 A KR20200068181 A KR 20200068181A
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- 239000011572 manganese Substances 0.000 title claims abstract description 92
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 title claims abstract description 90
- 229910052748 manganese Inorganic materials 0.000 title claims abstract description 89
- 238000000034 method Methods 0.000 title claims abstract description 66
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 49
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 46
- 238000005259 measurement Methods 0.000 claims abstract description 27
- 238000002156 mixing Methods 0.000 claims abstract description 22
- 238000011088 calibration curve Methods 0.000 claims abstract description 19
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims abstract description 17
- 230000008569 process Effects 0.000 claims abstract description 17
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- 235000011152 sodium sulphate Nutrition 0.000 claims abstract description 14
- 239000012086 standard solution Substances 0.000 claims abstract description 14
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims abstract description 11
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- 229910000162 sodium phosphate Inorganic materials 0.000 claims abstract description 8
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- OXTNCQMOKLOUAM-UHFFFAOYSA-N 3-Oxoglutaric acid Chemical compound OC(=O)CC(=O)CC(O)=O OXTNCQMOKLOUAM-UHFFFAOYSA-N 0.000 description 1
- AYKYXWQEBUNJCN-UHFFFAOYSA-N 3-methylfuran-2,5-dione Chemical compound CC1=CC(=O)OC1=O AYKYXWQEBUNJCN-UHFFFAOYSA-N 0.000 description 1
- OFNISBHGPNMTMS-UHFFFAOYSA-N 3-methylideneoxolane-2,5-dione Chemical compound C=C1CC(=O)OC1=O OFNISBHGPNMTMS-UHFFFAOYSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
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- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
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- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- WAEMQWOKJMHJLA-UHFFFAOYSA-N Manganese(2+) Chemical compound [Mn+2] WAEMQWOKJMHJLA-UHFFFAOYSA-N 0.000 description 1
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- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
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- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 150000001735 carboxylic acids Chemical class 0.000 description 1
- GTZCVFVGUGFEME-IWQZZHSRSA-N cis-aconitic acid Chemical compound OC(=O)C\C(C(O)=O)=C\C(O)=O GTZCVFVGUGFEME-IWQZZHSRSA-N 0.000 description 1
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- 230000001939 inductive effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- IEECXTSVVFWGSE-UHFFFAOYSA-M iron(3+);oxygen(2-);hydroxide Chemical compound [OH-].[O-2].[Fe+3] IEECXTSVVFWGSE-UHFFFAOYSA-M 0.000 description 1
- YHGPYBQVSJBGHH-UHFFFAOYSA-H iron(3+);trisulfate;pentahydrate Chemical compound O.O.O.O.O.[Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O YHGPYBQVSJBGHH-UHFFFAOYSA-H 0.000 description 1
- 229910000360 iron(III) sulfate Inorganic materials 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000002535 lyotropic effect Effects 0.000 description 1
- KQFUCKFHODLIAZ-UHFFFAOYSA-N manganese Chemical compound [Mn].[Mn] KQFUCKFHODLIAZ-UHFFFAOYSA-N 0.000 description 1
- 239000000463 material Substances 0.000 description 1
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- 238000012544 monitoring process Methods 0.000 description 1
- 235000014571 nuts Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
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- 150000004686 pentahydrates Chemical class 0.000 description 1
- HWGNBUXHKFFFIH-UHFFFAOYSA-I pentasodium;[oxido(phosphonatooxy)phosphoryl] phosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O HWGNBUXHKFFFIH-UHFFFAOYSA-I 0.000 description 1
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- 229910052979 sodium sulfide Inorganic materials 0.000 description 1
- GRVFOGOEDUUMBP-UHFFFAOYSA-N sodium sulfide (anhydrous) Chemical compound [Na+].[Na+].[S-2] GRVFOGOEDUUMBP-UHFFFAOYSA-N 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/29—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/1813—Specific cations in water, e.g. heavy metals
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
Description
본 발명은 망간(Manganese)농도 검출 방법에 관한 것으로, 더욱 구체적으로는 측정하고자 하는 망간(Manganese)를 함유한 시료에 망간결합 발색제(Manganese Coloring Reagent)를 이용하여 망간(Manganese)농도를 산출하는 망간(Manganese)농도 검출 방법에 관한 것이다.The present invention relates to a method for detecting manganese concentration, and more specifically, manganese for calculating manganese concentration using a manganese coloring agent in a sample containing manganese to be measured. (Manganese) It relates to a concentration detection method.
망간은 지각에 세 번째로 많이 포함된 전이금속이고 모든 원소 중에서는 열두 번째로 많다. 다른 전이금속들과 마찬가지로 망간도 모든 생명체에게 필수적인 원소지만 많은 양을 필요로 하지는 않는다. Manganese is the third-largest transition metal in the earth's crust and is the twelfth largest among all elements. Manganese, like other transition metals, is an essential element for all living things, but does not require large amounts.
효소들이 제대로 기능하기 위해서는 망간 원자가 있어야 한다. 식물에서 일어나는 광합성 작용의 일부인 물을 분해하여 산소를 방출하는 ‘산소 방출 복합체’도 그런 효소 중 하나다. 사람에게는 망간이 연결 조직의 형성, 혈액 응고인자의 생성, 대사 작용의 조절과 같은 다양한 과정에 관여한다. Manganese atoms are required for enzymes to function properly. One of these enzymes is the “oxygen-releasing complex,” which releases oxygen by decomposing water, which is part of the photosynthetic action in plants. In humans, manganese is involved in a variety of processes, such as the formation of connective tissue, the formation of blood clotting factors, and the regulation of metabolism.
성인의 몸에는 약 12mg의 망간이 있으며 매일 음식물을 통해 5mg을 섭취해야 한다. 달걀, 잣, 올리브오일, 콩, 가재에 많으며 너무 많은 망간을 섭취하면 건강에 해가 될 수도 있다. 장기간 매일 10mg 이상의 망간을 섭취하면 신경 장애를 유발하고 철분 흡수를 방해하는 등 여러 가지 부작용이 나타날 수 있다. An adult's body has about 12mg of manganese and should consume 5mg daily through food. It is found in eggs, pine nuts, olive oil, soybeans, and crayfish, and eating too much manganese can harm your health. If you consume more than 10mg of manganese every day for a long period of time, it can cause neurological disorders and interfere with iron absorption.
최근 화학, 생명공학 및 환경공학적 공정에서의 중요성으로 인해 금속 이온을 검출할 수 있는 선택적인 검출제의 개발은 환경 및 생물학적 분야에서 큰 화두로 떠오르고 있다. 하나 이상의 금속이온을 검출할 수 있는 단일 화합물을 개발하려는 노력이 많이 이루어지고 있고, 여러 금속이온들을 형광이나 색 변화와 같이 다른 검출방법으로 검출할 수 있는 장치의 개발 역시 많이 연구되고 있다. 그 중에서도 형광센서는 높은 민감도와 선택성, 빠른 반응 속도 및 간단한 조작 방법 등의 장점을 가지므로 훌륭한 이온 검출 도구로 알려져 있다. 또한, 색 변화 화학센서 역시 가격이 저렴하고 육안으로 쉽게 표적 이온을 탐지할 수 있는 등 검출을 위한 별도의 기기를 필요로 하지 않는다는 장점으로 인해 상당한 관심을 받고 있는 추세이다.Due to the importance in chemical, biotechnological and environmental engineering processes, the development of selective detection agents capable of detecting metal ions has become a hot topic in the environment and biological fields. Efforts have been made to develop a single compound capable of detecting one or more metal ions, and development of a device capable of detecting various metal ions by different detection methods such as fluorescence or color change has also been studied. Among them, the fluorescence sensor is known as a good ion detection tool because it has advantages such as high sensitivity, selectivity, fast reaction speed, and simple operation method. In addition, the color change chemical sensor is also in the trend of receiving considerable attention due to the advantage that it does not require a separate device for detection, such as low cost and easy detection of target ions by the naked eye.
본 발명과 관련된 기술로 등록특허(10-1618448호, 이하 선행기술)는 "줄로리딘계 화합물, 이를 이용한 망간이온, 아연이온 및 알루미늄 이온 검출제, 검출방법 및 검출장치"로서,As a technology related to the present invention, the registered patent (10-1618448, hereinafter referred to as prior art) is a "zurolidine-based compound, a manganese ion, zinc ion and aluminum ion detection agent, detection method and detection device using the same"
"8-하이드록시줄로리딘-9-카복스알데하이드 및 2,2'-옥시비스(에틸아민)을 반응시켜서 제조한 줄로리딘 화합물로 망간이온을 검출하는 방법"을 제공한바 있다."A method for detecting manganese ions with a girolidine compound prepared by reacting 8-hydroxyzoloridine-9-carboxaldehyde and 2,2'-oxybis(ethylamine)" has been provided.
상기한 종래기술 및 선행기술은 시료의 망간 농도를 측정하는데 다양한 시약과 복잡한 절차로 측정과정이 매우 복잡하고 또한 다양한 시약을 사용함에 따라 그 비용면에서도 경제적이지 못할 뿐만 아니라 측정시간이 오래 걸리는 단점이 있다.The above-mentioned prior art and prior art measure a manganese concentration of a sample, and the measurement process is very complicated with various reagents and complicated procedures, and the use of various reagents is not economical in terms of its cost, but also has a disadvantage that it takes a long measurement time. have.
따라서 본 발명은 본 발명은 다양한 시약과 복잡한 절차로 측정과정이 매우 복잡하고 또한 다양한 시약을 사용함에 따라 그 비용면에서도 비경제적인 면 그리고 측정시간이 오래 걸리는 단점을 해결하는 망간 농도를 측정할 수 있는 방법을 제공하고자 한다.Therefore, the present invention can measure the concentration of manganese, which solves the disadvantages of costly and inefficient economical aspects of the measurement process by using various reagents and complicated procedures. I want to provide a method.
또한 종래기술 및 선행기술은 망간 농도를 검출하는 검출시약을 이용하여 망간 농도를 측정하는 경우 시료 중에 포함된 다른 종류의 이온에 의한 간섭(Interfere)의 영향이 많아 측정의 정확도가 떨어지는 문제점이 있다.In addition, in the prior art and the prior art, when measuring the concentration of manganese using a detection reagent that detects the concentration of manganese, there is a problem in that the accuracy of the measurement is deteriorated due to the influence of interference by different types of ions included in the sample.
본 발명은 상기한 문제점 및 요구를 해결하기 위하여,The present invention to solve the above problems and needs,
측정하고자 하는 망간이 포함된 시료와 망간 농도 검출 시약을 혼합하여 발색시키는 과정(1과정),The process of mixing the sample containing manganese to be measured and the manganese concentration detection reagent to develop color (step 1),
표준액에 대한 검량선을 작성하는 과정(2과정),The process of preparing a calibration curve for the standard solution (2 steps),
측정용기에 상기한 시료와 망간 농도 검출 시약을 혼합하여 발색된 측정 시료를 측정용기에 넣어 시료에 포함된 망간의 농도를 측정하는 과정(3과정)을 포함하는 망간 농도 측정 방법을 제공한다.It provides a method for measuring the concentration of manganese, including a process (step 3) of measuring the concentration of manganese contained in the sample by mixing the sample and the manganese concentration detection reagent in the measuring container and putting the colored measurement sample in the measuring container.
또한 본 발명은 상기한 망간 농도 검출 시약은 소디움 포스페이트, 시트릭 애씨드, 소디움 설페이트를 혼합하여 조성한 망간 농도 검출 시약인 것을 특징으로 하는 망간 농도 측정 방법을 제공한다.In addition, the present invention provides a method for measuring manganese concentration, wherein the above-described manganese concentration detection reagent is a mixture of sodium phosphate, citric acid, and sodium sulfate, and is a manganese concentration detection reagent.
또한 본 발명은 상기한 3과정의 측정용기에 상기한 시료와 망간 농도 검출 시약을 혼합하여 발색된 측정 시료를 측정용기에 넣어 시료에 포함된 망간의 농도를 측정하는 과정은 흡광광도법 또는 비색표대비를 통하여 측정하는 것을 특징으로 하는 망간 농도 측정 방법을 제공한다.In addition, in the present invention, the process of measuring the concentration of manganese contained in a sample by mixing the sample and the manganese concentration detection reagent in the measuring vessel of the above-described three steps and putting the colored measurement sample in the measuring container is compared with the absorbance method or the colorimetric table. It provides a method for measuring the concentration of manganese, characterized in that through the measurement.
본 발명에 따른 망간 농도 검출 방법은 망간 측정시에 발생할 수 있는 다른 종류의 이온에 의한 간섭의 영향을 현저히 배제할 수 있는 효과가 나타난다.The method for detecting the concentration of manganese according to the present invention has an effect of remarkably excluding the effect of interference by other types of ions that may occur during manganese measurement.
또한 본 발명에 따른 망간 농도 검출 방법에서 사용하는 망간 농도 검출시약을 사용하여 측정하는 경우 망간(Manganese)과 망간 농도 검출 시약의 반응시간을 획기적으로 단축하여 과도한 측정시간을 요하지 않고 그 절차도 매우 간단히 수행할 수 있는 효과가 나타난다.In addition, when measuring using the manganese concentration detection reagent used in the method for detecting manganese concentration according to the present invention, the reaction time of manganese and manganese concentration detection reagents is significantly shortened, and the procedure is very simple without requiring excessive measurement time. The effect can be achieved.
또한 본 발명에 따른 망간 농도 검출 방법을 이용하여 망간 농도를 측정할 경우 기존의 측정방법과 대비하여 동등 이상의 정확도를 확보할 수 있는 효과를 나타낸다.In addition, when measuring the concentration of manganese using the method for detecting the concentration of manganese according to the present invention, it shows an effect capable of securing an accuracy equal to or greater than that of the conventional measurement method.
도 1은 본 발명에 따른 표준액에 대한 검량선의 일 실시예.1 is an embodiment of a calibration curve for a standard solution according to the present invention.
이하 본 발명을 도면을 참고하여 상세히 설명하고자 한다.Hereinafter, the present invention will be described in detail with reference to the drawings.
본 발명은 시료에 포함되어 있는 망간의 농도를 측정하기 위한 대표적인 방법인 흡광광도법 또는 비색대비법을 이용하여 망간의 농도를 검출하는 방법에 관한 발명이다.The present invention relates to a method for detecting the concentration of manganese using an absorbance method or a colorimetric contrast method, which are representative methods for measuring the concentration of manganese contained in a sample.
종래의 수질환경보전법에 의한 망간의 측정방법은 아래의 3가지 방법으로 측정하게 된다.The method of measuring manganese by the conventional water environment conservation method is measured by the following three methods.
1. 원자흡광광도법1. Atomic Absorption Spectroscopy
1.1 측정원리1.1 Measuring principle
망간을 원자흡광광도법에 따라 정량하는 방법이다.It is a method of quantifying manganese according to atomic absorption spectrometry.
정량범위는 사용하는 장치 및 측정조건에 따라 다르지만 279.5㎚에서 0.1～4㎎/ℓ이고 표준편차율은 10～2%이다. 이 방법에 따라 시험할 경우 유효측정농도는 0.005㎎/ℓ 이상으로 한다.The quantitative range varies depending on the device and measurement conditions used, but is 0.1 to 4 mg/ℓ at 279.5 nm and the standard deviation rate is 10 to 2%. When tested according to this method, the effective measurement concentration is 0.005 mg/L or more.
1.2 기구 및 기기1.2 Instruments and appliances
㈎ 원자흡광 분석장치㈎ Atomic Absorption Analysis Device
㈏ 램프:망간중공음극램프㈏ Lamp: Manganese hollow cathode lamp
㈐ 가스:가연성가스:아세틸렌Gas: Combustible gas: Acetylene
조연성가스:공기Combustible gas: Air
1.3 시료의 전처리1.3 Sample preparation
㈎ 제2장 제4항 시료의 전처리 방법에 의한다.장 According to
㈏망간함유량이 미량일 경우에는 시료 적당량을 비커에 넣어 가열하여 액온을 90℃로 하고 황산제이철 용액(Fe3 +, 2㎎/㎖) 5㎖와 과산화수소수(30%) 5～10㎖를 넣는다. 10% 수산화나트륨용액 또는 암모니아수(1+1)를 혼화하면서 넣어 생성된 수산화제이철 침전을 여과한다.일 When the manganese content is very small, put the appropriate amount of sample in a beaker and heat it to bring the liquid temperature to 90℃, and add 5 ml of ferric sulfate solution (Fe 3 + , 2 mg/ml) and 5-10 ml of hydrogen peroxide (30%). . The resulting ferric hydroxide precipitate is filtered by mixing with 10% sodium hydroxide solution or ammonia water (1+1).
침전을 온수로 씻고 과산화수소수(3%) 소량을 넣은 염산(1+2) 소량으로 녹이고 거름종이를 온수로 씻어 준다. 이액 및 씻은 액을 합하여 0.1～1N의 염산산성으로 하여 일정량으로 한다.The precipitate is washed with hot water, dissolved in a small amount of hydrochloric acid (1+2) with a small amount of hydrogen peroxide (3%), and the filter paper is washed with hot water. The lyotropic liquid and the washed liquid are combined to give a hydrochloric acid of 0.1 to 1 N to a certain amount.
㈐용해성 망간을 측정할 경우에는 시료채취 즉시 여과하여 여액을 ㈎ 또는 ㈏에 따라 전처리 한다.When measuring soluble manganese, filter it immediately after sampling and pretreat the filtrate according to ㈎ or ㈏.
1.4 시험방법1.4 Test method
제3장 제2항 원자흡광광도법에 따라 279.5㎚에서 전처리한 시료용액의 흡광도를 측정하고 미리 작성한 검량선으로 부터 망간의 양을 구하고 농도(㎎/ℓ)를 산출한다. Chapter 3
바탕시험을 행하여 보정한다.Perform a background test to calibrate.
검량선의 작성 Preparation of calibration curve
망간 표준액(0.01㎎ Mn/㎖) 1～40㎖를 단계적으로 취하여 100㎖ 용량플라스크에 넣고 시료와 같은 양의 산을 넣어 물로 표선을 채운 다음 이하 시료의 시험방법에 따라 시험하고 망간의 농도와 흡광도와의 관계선을 작성한다.Manganese standard solution (0.01 mg Mn/ml) Take 1 to 40 ml step by step, put it in a 100 ml volumetric flask, add the same amount of acid as the sample, fill the mark with water, and then test according to the test method of the sample. Create a relationship line with.
2. 흡광광도법(과요오드산 칼륨법)2. Absorption spectrophotometry (potassium periodate)
2.1 측정원리2.1 Measuring principle
망간이온을 황산산성에서 과요오드산 칼륨으로 산화하여 생성된 과망간산 이온의 흡광도를 525㎚에서 측정하는 방법이다.This method measures the absorbance of permanganate ions produced by oxidizing manganese ions from sulfuric acid to potassium periodate.
정량범위는 0.04～0.5㎎이고 표준편차율은 10～3%이다.Quantitative range is 0.04~0.5mg and standard deviation rate is 10~3%.
2.2 기구 및 기기2.2 Instruments and appliances
광전광도계 또는 광전분광광도계Photophotometer or photospectrophotometer
2.3 시료의 전처리2.3 Sample preparation
㈎제2장 제4항 시료의 전처리 방법중 질산-황산에 의한 분해에 따른다.㈎ In
㈏망간 함유량이 미량일 경우에는 1.3 ㈏에 따라 시험하여 여과한 침전을 온수로 씻은 다음 침전물을 주의하여 비커에 옮기고, 거름종이에 붙어 있는 침전물을 소량의 과산화수소수(1+10)를 넣은 황산(1+9) 50㎖로 씻어서 녹이고 물로 거름종이를 씻어준다.경우 When the manganese content is very small, wash the filtered precipitate with hot water after testing in accordance with 1.3 다음, carefully transfer the precipitate to a beaker, and sulfuric acid containing a small amount of hydrogen peroxide (1+10) in the precipitate attached to the filter paper. 1+9) Wash with 50 ml, dissolve, and wash the filter paper with water.
여액과 씻은 액을 침전물이 들어 있는 비커에 넣고 가열하여 침전물을 녹인 다음 과산화수소수를 분해하기 위하여 액량 약 40㎖까지 가열 농축한다.The filtrate and the washed solution are placed in a beaker containing precipitate, heated to dissolve the precipitate, and concentrated to heat up to about 40 ml to dissolve the hydrogen peroxide solution.
㈐용해성 망간을 측정할 경우에는 시료채취 즉시 여과하여 여액을 ㈎또는 ㈏에 따라 전처리 한다.When measuring soluble manganese, filter it immediately after sampling and pretreat the filtrate according to ㈎ or ㈏.
2.4 시험방법2.4 Test method
시료(주1) 또는 전처리한 시료 적당량(망간으로서 0.5㎎ 이하)을 취하여 비커에 넣고 황산의 총량이 5㎖가 되도록 황산(1+1)을 넣어주고(주2) 가열하여 황산의 백연을 발생시킨다. 방냉한 다음 물 약 20㎖와 인산 1㎖를 넣고 가열하여 내용물을 녹인다. 불용물이 있을 경우에는 여과하여 온수로 씻어주고 여액과 씻은 액을 합하여 물을 넣어 약 45㎖로 한다. 여기에 과요오드산칼륨 0.5g을 넣고 끓는 물중탕 중에서 정확히 30분간 가열하여 발색시킨다.Take a sample (Note 1) or an appropriate amount of pre-treated sample (0.5 mg or less as manganese), put it in a beaker, add sulfuric acid (1+1) so that the total amount of sulfuric acid is 5 ml (Note 2) and heat it to generate white smoke of sulfuric acid. Order. After cooling, add about 20 ml of water and 1 ml of phosphoric acid, and heat to dissolve the contents. If there is insoluble matter, filter it, wash it with hot water, add the filtrate and the washed solution, and add water to make 45 ml. Add 0.5 g of potassium periodate to it and heat it in boiling water bath for exactly 30 minutes.
속히 물로 냉각한 다음 50㎖ 용량플라스크에 옮겨 물로 표선을 채운다. 이 용액 일부를 층장 10㎜ 흡수셀에 옮겨 시료용액으로 하고, 따로 물 약 30㎖를 취하여 황산(1+1) 10㎖와 인산 1㎖를 넣어준 다음 시료의 시험방법에 따라 시험하여 바탕시험액으로 한다. 바탕시험액을 대조액으로 하여 525㎚에서 시료용액의 흡광도를 측정하고 미리 작성한 검량선으로 부터 망간의 양을 구하여 농도(㎎/ℓ)를 산출한다.Cool quickly with water, then transfer to a 50 ml volumetric flask to fill the markings with water. A part of this solution is transferred to a 10 mm absorbent cell in a layer length to be used as a sample solution. Separately, about 30 ml of water is taken, 10 ml of sulfuric acid (1+1) and 1 ml of phosphoric acid are added, and then tested according to the test method of the sample to serve as the background test solution. do. Using the background test solution as a control solution, measure the absorbance of the sample solution at 525 nm and calculate the concentration (mg/ℓ) by obtaining the amount of manganese from a previously prepared calibration curve.
검량선의 작성 Preparation of calibration curve
망간 표준액(0.02㎎ Mn/㎖) 2～25㎖를 단계적으로 취하여 비커에 넣고 물을 넣어 약 30㎖로 한 다음 황산(1+1) 10㎖ 및 인산 1㎖를 넣어 이하 시료의 시험방법에 따라 시험하고 망간의 양과 흡광도와의 관계선을 작성한다.Manganese standard solution (0.02 mg Mn/ml) 2-25 ml is taken step by step, placed in a beaker, water is added to about 30 ml, then 10 ml of sulfuric acid (1+1) and 1 ml of phosphoric acid are added according to the test method of the sample below. Test and draw the relationship between the amount of manganese and absorbance.
주1)시료중 이 시험방법에 영향이 큰 유기물질이나 기타 방해물질이 존재하지 않을 경우에는 전처리를 생략 할 수도 있다.Note 1) If there are no organic substances or other interfering substances in the sample that have a large influence on this test method, the pretreatment may be omitted.
2)시료를 전처리 하지 않고 시험할 경우에는 황산(1+1) 10㎖를 넣어주며, 2.3 ㈏에 따라 전처리 한 시료로서 전처리 한 용액 전량을 취하여 시험할 경우에는 황산을 넣어주지 않는다.2) If the sample is tested without pre-treatment, 10 ml of sulfuric acid (1+1) is added. As a sample pre-treated according to 2.3 ㈏, the total amount of the pre-treated solution is taken and sulfuric acid is not added.
3. 유도결합플라스마 발광광도법3. Inductively coupled plasma emission photometric method
3.1 측정원리3.1 Measuring principle
망간을 유도결합플라스마 발광광도법에 따라 정량하는 방법이다. 정량범위는 사용하는 장치 및 측정조건에 따라 다르지만 257.61㎚에서 0.002～50㎎/ℓ이다.It is a method of quantifying manganese according to an inductively coupled plasma emission photometric method. The quantitative range varies depending on the device used and the measurement conditions, but is 0.002-50 mg/l at 257.61 nm.
3.2 기구 및 기기3.2 Instruments and appliances
㈎ 유도결합플라스마 발광광도분석장치㈎ Inductively coupled plasma luminescence intensity analyzer
㈏ 아르곤 가스:액화 또는 압축아르곤으로서 99.99V/V% 이상㈏ Argon gas: 99.99V/V% or more as liquefied or compressed argon
3.3 시료의 전처리3.3 Sample preparation
㈎ 제2장 제4항 시료의 전처리 방법에 의한다.장 According to
㈏용해성 망간을 측정할 경우에는 시료채취 즉시 여과하여 여액을 ㈎에 따라 전처리하여 시료로 한다.측정 When measuring soluble manganese, filter it immediately after sampling and pretreat the filtrate according to ㈎.
3.4 시험방법3.4 Test method
제3장 제3항 유도결합플라스마 발광광도법에 따라 257.61㎚에서 시료용액의 발광강도를 측정하고 미리 작성한 검량선으로부터 망간의 양을 구하여 농도(㎎/ℓ)를 산출한다.Chapter 3 Paragraph 3 Measure the luminescence intensity of the sample solution at 257.61 nm according to the inductively coupled plasma luminescence photometric method, and calculate the concentration (mg/ℓ) by obtaining the amount of manganese from a previously prepared calibration curve.
바탕시험을 행하여 보정한다.Perform a background test to calibrate.
검량선의 작성Preparation of calibration curve
망간 표준액(0.05㎎ Mn/㎖) 0, 2, 10, 20㎖를 정확히 취하여 100㎖ 용량플라스크에 넣고 질산(1+1) 2㎖, 염산(1+1) 10㎖ 및 물을 넣어 표선을 채운 다음 이하 시료의 시험방법에 따라 시험하여 망간의 농도와 발광광도와의 관계선을 작성한다.Manganese standard solution (0.05mg Mn/ml) Take exactly 0, 2, 10, 20ml, put it into a 100ml volumetric flask, add 2ml of nitric acid (1+1), 10ml of hydrochloric acid (1+1) and water to fill the mark. The following test is performed according to the test method of the sample to prepare the relationship line between the concentration of manganese and the luminous intensity.
본 발명은 측정하고자 하는 망간이 포함된 시료와 망간 농도 검출 시약을 혼합하여 발색시키는 과정을 수행한다.(1과정)The present invention performs a process of coloring by mixing a sample containing manganese to be measured and a manganese concentration detection reagent. (Step 1)
망간이 포함된 시료는 10 중량부에 망간 농도 검출 시약은 20~120 중량부를 혼합하는 것이 시료의 발색이 잘 되는 효과가 나타난다.When the sample containing manganese is mixed with 20 to 120 parts by weight of the manganese concentration detection reagent, the color of the sample is well exhibited.
상기한 망간이 포함된 시료와 망간 농도 검출 시약을 혼합하여 반응을 시키는 시간은 10~20분 바람직하게는 15분 정도로 하는 것이 좋다.The time for mixing the manganese-containing sample and the manganese concentration detection reagent to react is preferably 10 to 20 minutes, preferably about 15 minutes.
본 발명의 기술적 특징은 상기한 망간 농도 검출 시약이 방해 이온의 간섭없이 망간 농도를 잘 측정할 수 있는 시약으로 된 점이다.The technical feature of the present invention is that the above-described manganese concentration detection reagent is a reagent capable of measuring manganese concentration well without interference of interfering ions.
본 발명의 검출시약은 소디움 포스페이트, 시트릭 애씨드, 소디움 설페이트를 혼합하여 조성한 것을 특징으로 한다.The detection reagent of the present invention is characterized by mixing sodium phosphate, citric acid, and sodium sulfate.
본 발명의 소디움 포스페이트(Sodium phosphate)는 제삼인산나트륨이라고도 한다. 넓은 의미로는, Na2HPO4ㆍ12H2O(인산수소이나트륨)를 포함하는 경우도 있다. 수화물 Na3HPO4ㆍNH2O에서는 n이 0.5, 6, 10, 12의 것이 있다. 인산수소이나트륨의 수용액에 적정량의 수산화나트륨을 가해 얻을 수 있는 생성물을 실온에서 재결정하면 십이수화물을 얻을 수 있다. 열수로부터는 육수화물을 얻을 수 있다. 또 가열(180℃)에 의해 무수염이 된다. 이들 모두 백색에서 무색의 고체, 수용액은 강알칼리성(수소염은 미알칼리성)을 띈다. 물 100g에 대한 용해도는, 14.5g(25℃ 무수염으로써), 도금에서는 탈지세정제, 주석도금 등의 도금피막의 변색 방지제로써 이용된다.Sodium phosphate of the present invention is also referred to as sodium triphosphate. In a broad sense, Na2HPO4·12H2O (disodium hydrogen phosphate) may also be included. In hydrate Na3HPO4.NH2O, n is 0.5, 6, 10, 12. A product obtained by adding an appropriate amount of sodium hydroxide to an aqueous solution of disodium hydrogen phosphate is recrystallized at room temperature, whereby a dihydrate can be obtained. The hexahydrate can be obtained from hot water. Moreover, it becomes an anhydrous salt by heating (180 degreeC). All of them are white to colorless solid, and the aqueous solution has strong alkalinity (hydrogen salt is non-alkaline). The solubility in 100 g of water is 14.5 g (as an anhydrous salt at 25° C.), and is used as a color change preventing agent for plating films such as degreasing cleaners and tin plating in plating.
본 발명의 시트릭 애씨드(citric acid)는 하이드록시기(-OH)를 가지는 다염기(多鹽基) 카복실산의 하나로 구연산(枸祁酸)이라고도 한다. 화학식 C6H8O7. 많은 식물의 씨나 과즙 속에 유리상태의 산으로 함유되어 있다. 구연이란 시트론 citron의 한자명이며, 시트론을 비롯하여 레몬이나 덜 익은 감귤 등 감귤류의 과일에 특히 많이 함유되어 있는 데에서 연유한다. 영어명인 citric acid의 citric도 감귤류를 뜻하는 그리스어인 citrus에서 유래한 것이다. 물에서 결정시키면 1분자의 결정수를 지닌 큰 주상 결정이 생긴다. 가열하면 무수물(無水物)이 되는데, 이것은 녹는점이 153℃이다. 온도를 더 올리면 175℃에서 아코니트산이 되고, 고온에서는 아이타콘산 무수물이나 전위 생성물인 시트라콘산 무수물 및 아세톤다이카복실산을 생성한다. 물·에탄올에 잘 녹는다. 당류를 기질로 하여 미생물을 배양했을 때, 배양액 속에 시트르산이 축적되는 현상을 볼 수 있는데 이것을 시트르산발효라 한다. 여러 배양 방법이 연구되어 지금은 세계에서 생산되는 시트르산 총량의 90%가 이 발효법에 의해서 만들어진다. 시트르산발효를 일으키는 미생물로는 보통 검정곰팡이가 사용되는데, 산성(pH 2∼3)에서 약 30℃, 7∼10일간 발효시키면 시트르산을 얻을 수 있다. 또, TCA 회로를 구성하는 한 요소로 시트르산은 고등동물의 물질대사에서 중요한 구실을 한다. 또한, 체내의 칼슘 흡수를 촉진시키는 작용도 알려져 있다. 용도로는 과즙·청량음료에 첨가하거나, 의약품·이뇨성 음료에 신맛을 내는 외에 분석 시약으로도 사용된다. 또, 혈액 응고에는 칼슘이온이 필요한데, 시트르산은 칼슘이온을 포착(捕捉)하므로 혈액응고저지제로 사용된다.The citric acid of the present invention is also referred to as citric acid as one of the polybasic carboxylic acids having a hydroxy group (-OH). Formula C6H8O7. It is contained as a free acid in the seeds or juices of many plants. Citrus is a Chinese character for citron citron, and is derived from citron, as it is particularly contained in citrus fruits such as lemons and underripe citrus fruits. The citric acid citric acid in English is also derived from the Greek citrus, citrus. Crystallization in water results in large columnar crystals with one molecule of crystallized water. When heated, it becomes anhydrous, which has a melting point of 153°C. When the temperature is further increased, it becomes aconitic acid at 175°C, and at high temperatures, itaconic acid anhydride or translocation product citraconic anhydride and acetone dicarboxylic acid are produced. It is soluble in water and ethanol. When a microorganism is cultured using a saccharide as a substrate, citric acid accumulates in the culture medium. This is called fermentation of citric acid. Several culture methods have been studied and now 90% of the total amount of citric acid produced in the world is produced by this fermentation method. As a microorganism that causes fermentation of citric acid, black mold is usually used, and if it is fermented at about 30°C for 7 to 10 days in acidic (pH 2-3), citric acid can be obtained. In addition, citric acid plays an important role in the metabolism of higher animals as a component of the TCA circuit. In addition, it is also known to promote the absorption of calcium in the body. In addition, it is used as analytical reagents in addition to adding juice to soft drinks and sour to pharmaceuticals and diuretic beverages. In addition, calcium ions are required for blood clotting. Citric acid is used as a blood clotting inhibitor because it captures calcium ions.
본 발명의 소디움 설페이트(Sodium sulphate)는 황산나트륨으로 나트륨의 황산염로 무색의 결정이다. 공업적으로 비스코스 인견(人絹) 제조 때에 사용되는 방사욕 속의 황산나트륨을 4∼7℃로 냉각하여 결정을 석출시키고 분리 ·탈수하여 제품화하며, 유리나 황화나트륨의 제조 등에 사용된다. Sodium sulphate of the present invention is sodium sulfate, a colorless crystal of sodium sulfate. Industrially, sodium sulfate in the spinning bath used in the production of viscose human dogs is cooled to 4 to 7°C to precipitate crystals, separated and dehydrated, and then used for production of glass or sodium sulfide.
화학식 Na2SO4. 무수물은 무색 결정으로 분자량 142.02, 비중 2.698이다. 100g의 물에는 0℃에서 5g, 100℃에서 42g 녹으며, 알코올에는 녹지 않는다. 습한 공기 중에 방치하면 수분을 흡수하여 10수화물로 변한다. 10수화물 Na2SO4·10H2O는 비중 1.464이다. 건조한 공기 중에서는 풍해하여 무수물이 된다. 32.4℃로 가열하면 결정수에 녹아서 융해하고, 100℃로 가열하면 무수물이 된다. 100g의 물에 15℃에서 36g, 34℃에서 412g 녹으며, 무수물과 마찬가지로 알코올에는 녹지 않는다. 7수화물 Na2SO4·7H2O는 과포화 상태의 용액을 5℃ 이하로 냉각하거나 알코올을 가하면 얻는다.Chemical Formula Na2SO4. The anhydride is a colorless crystal with a molecular weight of 142.02 and a specific gravity of 2.698. It dissolves 5g at 0℃ and 42g at 100℃ in 100g of water, and insoluble in alcohol. When left in humid air, it absorbs moisture and turns into hexahydrate. The pentahydrate Na2SO4·10H2O has a specific gravity of 1.464. In dry air, it winds up and becomes anhydrous. When heated to 32.4°C, it melts and melts in crystalline water, and when heated to 100°C, it becomes an anhydride. It dissolves in 100g of water at 15℃ to 36g, and at 34℃ at 412g, and like anhydride, does not dissolve in alcohol. Heptahydrate Na2SO4·7H2O is obtained by cooling the supersaturated solution to 5°C or lower or adding alcohol.
본 발명은 바람직하게는 소디움 포스페이트 100중량부에 시트릭 애씨드 20~40중량부, 소디움 설페이트 40~70중량부를 혼합하여 조성한 망간 농도 측정 검출 시약이 측정 감도가 높고 측정 정확도가 높게 된다.The present invention is preferably a mixture of 20 to 40 parts by weight of citric acid and 40 to 70 parts by weight of sodium sulfate, and a manganese concentration measurement detection reagent composed of 100 parts by weight of sodium phosphate has high measurement sensitivity and high measurement accuracy.
상기한 소디움 포스페이트 100중량부에 시트릭 애씨드 20~40중량부, 소디움 설페이트 40~70중량부를 혼합하여 조성하면 환원 분말(Reduction Powder)이 되고, 이 환원 분말(Cadmium Reduction Powder) 일정 양과 망간(Manganese)함유 시료 수와 반응시킨 후 흡광광도법 또는 비색표 대조를 이용하여 망간(Manganese)의 농도를 산출하게 된다.When the composition is prepared by mixing 20 to 40 parts by weight of citric acid and 40 to 70 parts by weight of sodium sulphate, 100 parts by weight of sodium phosphate is reduced powder, and a certain amount of Cadmium Reduction Powder and manganese (Manganese) ) After reacting with the number of samples containing, the concentration of manganese is calculated using absorbance or colorimetric control.
본 발명은 표준액에 대한 검량선을 작성하는 과정을 수행한다.(2과정)The present invention performs a process of preparing a calibration curve for a standard solution.
검량선은 단계별로 망간 농도가 제시되어 있는 시료에 혼합하여 본 발명의 검출시약을 첨가 혼합하여 작성한다.The calibration curve is prepared by mixing the sample with the manganese concentration presented step by step and adding and mixing the detection reagent of the present invention.
본 발명은 측정용기에 상기한 시료와 망간 농도 검출 시약을 혼합하여 발색된 측정 시료를 측정용기에 넣어 측정파장의 흡광도를 측정하고 상기한 표준액에 대한 검량선과 대비하여 시료에 포함된 망간의 농도를 측정하는 과정을 수행하게 된다.(3과정)The present invention is to measure the absorbance of the measurement wavelength by mixing the sample and the manganese concentration detection reagent in a measuring container and putting a colored sample into the measuring container, and compare the concentration of manganese contained in the sample against the calibration curve for the standard solution. Measurement process will be performed.
본 발명에서 사용하는 흡광광도법은 이미 앞에서 언급한 바와 같이 시료 용액 중의 목적 성분을 적당한 시약으로 광흡수 물질로 변화시켜 특정 파장에서 광흡수 정도를 측정하여 목적 성분의 농도를 구하는 통상의 방법을 의미한다.The absorbance method used in the present invention means a conventional method of obtaining the concentration of the target component by measuring the degree of light absorption at a specific wavelength by changing the target component in the sample solution to a light absorbing material with a suitable reagent, as already mentioned above. .
본 발명의 흡광광도법은 람버트 비어 법칙을 적용하여 목적 성분의 농도를 구하는 것으로 통상의 흡광광도 분석장치는 통상 광원부, 파장선택부, 시료부, 측광부 등으로 이루어져 있다.The absorbance method of the present invention is to obtain the concentration of the target component by applying the Lambert Beer law, and a conventional absorbance analysis device is usually composed of a light source unit, a wavelength selection unit, a sample unit, and a metering unit.
흡광광도법에서 농도를 측정하는 방법으로는 색의 발색정도를 이용하는 비색법, 시료 용액의 현탁정도를 이용하는 비탁법 등이 있다. 흡광광도법은 시료액에 대응하는 대조액을 만들어 흡광도를 측정하고 이에 따라 검량선을 작성한 후 시료액의 흡광광도를 측정하여 상기 검량선과 비교하여 농도를 측정하게 된다.As a method for measuring the concentration in the absorbance method, there are a colorimetric method using a color development degree, and a turbidity method using a suspension degree of a sample solution. The absorbance method measures the absorbance by making a control solution corresponding to the sample solution, prepares a calibration curve accordingly, measures the absorbance of the sample solution, and measures the concentration compared to the calibration curve.
본 발명에서는 상기 비색법, 비탁법 모두 다 사용이 가능하며 바람직하게는 비색법으로 하는 것이 정확도가 높다. In the present invention, both the colorimetric method and the turbidity method can be used, and preferably, the colorimetric method has high accuracy.
또한 비색표 대조법은 다음과 같다.The colorimetric control method is as follows.
상기한 바와 같이 목적하는 성분을 여러 가지 농도에 따라 표준액을 만들고 이 표준액에 대하여 본 발명의 망간 농도 검출시약을 투입하여 기준 비색표를 설정한다. As described above, a standard solution is prepared according to various concentrations of a desired component, and a reference colorimetric table is set by introducing the manganese concentration detection reagent of the present invention to the standard solution.
그리고 시료에 상기한 망간 농도 검출시약을 혼합하여 혼화 반응시킨 나온 발색정도를 상기 기준 비색표와 비교하여 시료액의 농도를 측정하는 방법을 의미한다.And it refers to a method of measuring the concentration of the sample solution by comparing the color development degree obtained by mixing and reacting with the above-described manganese concentration detection reagent in the sample with the reference colorimetric table.
따라서 본 발명은 본 발명의 망간 농도 검출시약을 시료에 혼화 반응시켜 그 발색하는 정도를 이용하여 그 농도를 측정하는 그 어떤 방법도 본 발명의 기술적 내용에 포함된다 할 것이다.Therefore, the present invention will be included in the technical content of the present invention any method for measuring the concentration of the manganese concentration detection reagent of the present invention by mixing and reacting with the sample.
<실시예><Example>
1. 망간 농도 측정 검출 시약의 조성1. Composition of manganese concentration measurement detection reagent
<시약1> <Reagent 1>
Synonyms: Sodium phosphate Synonyms: Sodium phosphate
CAS No.: 7758-79-4 CAS No.: 7758-79-4
<시약2><
Synonyms: Citric acid Synonyms: Citric acid
CAS No.: 77-92-9 CAS No.: 77-92-9
<시약3><Reagent 3>
Synonyms: Sodium sulfate Synonyms: Sodium sulfate
CAS No.: 7757-82-6 CAS No.: 7757-82-6
을 혼합하여 망간 농도 측정 검출 시약을 조성한다.Mixing to form a manganese concentration measurement detection reagent.
상기한 시약별 조합 구성비는 다음과 같다. The combination composition ratio for each reagent is as follows.
2. 망간 농도 검출 방법2. Manganese concentration detection method
(1)흡광광도법 이용(1) Using absorbance photometry
1) 망간이 포함된 시료 10ml에 상기 준비된 검출시약 70mg을 혼합하여 반응을 시켜서 발색을 유도한다.1) A mixture of 70 mg of the prepared detection reagent is mixed with 10 ml of a sample containing manganese to react to induce color development.
반응시간은 약 15분 정도로 하였다.The reaction time was about 15 minutes.
상기의 시료를 흡광광도법을 이용하여 측정한다.The above sample is measured using an absorbance method.
측정파장 : 430nm 이고 측정용기는 직경 1 Inch 원형 유리병이다.Measuring wavelength: 430nm, measuring container is a diameter 1 Inch round glass bottle.
2)표준액에 대한 검량선을 작성한다.2) Prepare a calibration curve for the standard solution.
검량선은 단계별로 망간 농도가 제시되어 있는 시료에 혼합하여 본 발명의 검출시약을 첨가 혼합하여 작성한다.The calibration curve is prepared by mixing the sample with the manganese concentration presented step by step and adding and mixing the detection reagent of the present invention.
표준액에 대한 대조액 검량선은 도 1에 나타난 바와 같다.The control solution calibration curve for the standard solution is as shown in FIG. 1.
상기한 대조액 검량선과 시료에 대한 흡광광도를 비교하여 정확한 망간 농도를 측정하게 된다.An accurate manganese concentration is measured by comparing the absorbance of the control solution calibration curve and the sample.
(2) 비색표 대비법(2) Colorimetric contrast method
상기한 바와 같이 표준액에 대한 비색표를 만들어 상기한 발색이 유발된 시료에 대한 발색 정도를 비교하여 망간 농도를 산출한다.As described above, a colorimetric table for the standard solution is prepared to compare the color development degree of the sample inducing the color development to calculate the manganese concentration.
본 발명은 아래의 [표 2]에서 보는 바와 같이 이온의 간섭의 영향을 배제할 수 있는 높은 한계치를 나타내고 있다.As shown in [Table 2] below, the present invention shows a high limit value that can exclude the influence of ionic interference.
따라서 본 발명에 따른 망간 검출방법을 이용하는 경우 시료 중에 포함된 다른 종류의 이온에 의한 간섭의 영향을 현저히 감소시키는 효과가 있음을 알 수 있다.Therefore, it can be seen that when the manganese detection method according to the present invention is used, the effect of interference by other types of ions contained in the sample is significantly reduced.
본 발명은 상기한 구성과 기능으로 이루어진 망간 농도 검출 방법을 제공한다.The present invention provides a method for detecting manganese concentration having the above-described configuration and function.
본 발명은 수질환경기본법과 관련된 정수 및 하폐수처리사업, 공해방지업, 교육사업 등에 널리 사용가능하다.The present invention can be widely used in water purification and wastewater treatment projects related to the Framework Act on Water Quality, Pollution Prevention, and Education.
또한 본 발명은 공해방지모니터링업, 국가 및 지방정부의 환경정책관련업 등에 널리 이용가능하다.In addition, the present invention can be widely used in pollution prevention monitoring, environmental policy related industries of national and local governments, and the like.
이와 더불어, 수질분석약품사업, 수질분석약품제조업, 환경감리업, 환경모델링업, 환경영향평가업 및 교육용 자료 제공업 등에 매우 유용한 발명이다.In addition, it is a very useful invention in the water quality analysis chemicals business, water quality analysis chemicals manufacturing industry, environmental supervision business, environmental modeling business, environmental impact evaluation business, and educational data providing business.
본 발명은 특히 망간 농도를 측정하는 장치를 생산, 제조, 가공, 유통, 연구하는 산업에 매우 유용하다.The present invention is particularly useful in industries that produce, manufacture, process, distribute, and research devices that measure manganese concentration.
Claims (2)
표준액에 대한 검량선을 작성하는 과정(2과정),
측정용기에 상기한 시료와 망간 농도 검출 시약을 혼합하여 발색된 측정 시료를 측정용기에 넣어 시료에 포함된 망간의 농도를 측정하는 과정(3과정)을 포함하는 망간 농도 측정 방법.
The process of mixing the sample containing manganese to be measured and the manganese concentration detection reagent to develop color (step 1),
The process of preparing a calibration curve for the standard solution (2 steps),
A method for measuring the concentration of manganese, comprising the step of measuring the concentration of manganese contained in the sample by mixing the sample and the manganese concentration detection reagent in the measuring container and putting the colored measurement sample in the measuring container.
상기한 망간 농도 검출 시약은 소디움 포스페이트, 시트릭 애씨드, 소디움 설페이트를 혼합하여 조성한 망간 농도 검출 시약인 것을 특징으로 하는 망간 농도 측정 방법.
According to claim 1,
The manganese concentration detection reagent is a method for measuring the concentration of manganese, characterized in that a mixture of sodium phosphate, citric acid, sodium sulfate, and a concentration detection reagent.
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| CN113567367A (en) * | 2021-07-07 | 2021-10-29 | 合肥供水集团有限公司 | Method for rapidly detecting manganese content in water |
| CN114324314A (en) * | 2021-10-22 | 2022-04-12 | 徐慎怡 | Kit for quickly detecting aluminum element, preparation method thereof and quick detection method |
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