KR20200061000A - A composition for preventing light-induced damage comprising quercetin, genistein and alpha-lipoic acid - Google Patents

Abstract

The present invention relates to a composition for preventing photodamage containing quercetin, genistein and alpha lipoic acid. More specifically, the present invention relates to a composition for preventing and ameliorating skin damage due to ultraviolet rays, which contains quercetin, genistein and alpha lipoic acid as an active ingredient, and has an effect of suppressing the toxicity of skin cells caused by ultraviolet rays and alleviating symptoms of aging in skin cells caused by ultraviolet rays.

Description

A composition for preventing light-induced damage comprising quercetin, genistein and alpha-lipoic acid}

The present invention relates to a composition for preventing light damage containing quercetin, genistein, and alpha lipoic acid, and more specifically, quercetin, genistein, and alpha lipoic acid as active ingredients to suppress cytotoxicity caused by ultraviolet light and skin caused by light damage on the skin. It relates to a cosmetic composition exhibiting an excellent light damage preventing effect by showing a synergistic effect on alleviating aging symptoms.

Ultraviolet rays in sunlight cause light damage to the skin and adversely affect the beauty and skin health of the skin.

Light damage is a phenomenon that occurs when the skin is exposed to light and causes a photobiological reaction. It is divided into acute light damage that appears immediately after exposure to light and chronic light damage that occurs after long exposure to light. Chronic light damage may include light aging or skin cancer such as pigmentation or roughening of the skin, and acute light damage may include light burn or pigmentation.

Most of the light damage to the skin occurs in the epidermal layer and the upper layer of the dermis where ultraviolet rays penetrate, and the oxygen free radicals generated by ultraviolet irradiation are closely related to the light damage caused by ultraviolet rays such as pigmentation caused by ultraviolet rays, aging and skin cancer. Is known. Among the ultraviolet rays, ultraviolet ray B (UVB; 290-320 nm) is the main source of photo-biological effects on the human body and causes light damage to the skin, and ultraviolet ray C (UVC; 200-290 nm) is the power to destroy cells. This is very strong, but the wavelength is short, so the penetration power is weak, and the impact of UV A (UVA; 320-400 nm) is about 1/1000 of that of UVB, so its effect is minimal.

As part of a method for preventing skin light damage caused by ultraviolet rays, research on sunscreens has been continuously conducted. Most of the light damage preventing agents developed so far are applied to the skin, and agents that can reduce the absorption of light by absorbing, dispersing, or reflecting sunlight are used chemically or physically.

Previously developed sunscreens have the function of protecting cells by blocking the penetration of ultraviolet light, but it is impossible to suppress the effective light damage since UV light cannot inhibit the cell damage action after penetrating the epidermal layer and the upper layer of the dermis. . Research into effective compositions for suppressing light damage to skin exposed to UV light has been conducted, but the results have yet to be achieved.

Meanwhile, quercetin is a flavonoid system belonging to polyphenols, and is generally distributed as a glycoside of quercetin, such as vegetables and fruits. Usually bound to sugars such as quercetin-3-rutinoside or glucose (isoquercetrin) (Anderson and Coyle, (1994) Trends Pharmacol Sci 15:324-332; Faccioli et al., (1996) Mediators Inflamm 5 :24-31; Wills-Karp, (1999) Annu Rev Immunol 17:255-281). It has anti-hypertensive and anti-carcinogenic effects (Formica and Regelson, (1995) Food Chem Toxicol 33:1061-1080; Murota and Terao, (2003) Arch Biochem Biophys 417:12-17; Nakayama, (1994) Cancer Res 54 :1991s-1993s) (Kandaswami and Middleton, (1994) Adv Exp Med Biol 366:351-376; Robak and Gryglewski, (1988) Biochem Pharmacol 37:837-841). In addition, quercetin decreases Th2-induced-IL4 expression in normal peripheral blood mononuclear cells and has anti-inflammatory treatment effects in animal models of asthma, a complex inflammation-related abnormality (Dorsch et al., (1992) Int Arch Allergy Immunol 97:1-7; Rogerio et al., (2007) Inflamm Res 56:402-408).

In addition, genistein is one of isoflavones and is mainly distributed in legumes and has estrogen-like activity. Binds to estrogen receptors and inhibits tyrosine kinase activity (Filipeanu CM et al. Eur J Pharmacol 1995; 281: 29-35) and prevents breast cancer and prostate cancer (Whitsett Jr TG, Lamartiniere CA. Expert Rev Anticancer Ther 2006; 6 : 1699-1706; Sarkar FH et al. Mini Rev Med Chem 2006; 6: 401-407). Genistein also has anti-inflammatory properties by down-regulating cytokine-induced signaling in immune system cells (Hernandez-Montes E et al. Biochem Biophys Res Commun 2006; 346: 851-859; Verdrengh M et al. Inflamm Res 2003; 52: 341-346). Pretreatment of RAW264.7 macrophages with genistein inhibits the release of TNF-α (tumor necrosis factor-α) and IL-6 (interleukin-6) stimulated by LPS. In addition, genistein inhibits endotoxin-induced inflammatory responses in in vivo liver and intestine (Paradkar PN et al. Cancer Lett 2004; 215: 21-28). In addition, genistein has been found to modulate genes important for cell proliferation, cell cycle, apoptosis and transcription regulation (Cooke PS et al. J Nutr 2006; 136: 704-708).

In addition, alpha-lipoic acid (α-lipoic acid) is a fatty acid synthesized in cells of animals and plants, and has a chemical structure having 8 carbons and 2 sulfurs. Alpha lipoic acid is the latest ingredient attracting attention as an antioxidant, exfoliant, and anti-inflammatory agent, but it is an antioxidant that has been widely used in food for a long time. Alpha lipoic acid is absorbed into the stratum corneum of the skin in a very stable form and reduced to dihydrolipoic acid. Using the efficacy of this alpha-lipoic acid, it is currently used in the treatment of diabetes, neurological diseases, cataracts, heart attacks, atherosclerosis, and promotes ceramide production, so it has excellent moisturizing effect (US Patent No. 5472698) and skin It is effective for inflammation, necrosis, tumors and allergies (German Patent No. 441708), has an effect on allergies, inflammation, and tanning (International Publication WO 9508564), and has skin whitening and tyrosinase inhibitory effects. (Japanese Patent No. 6308315), it is known that it is effective in preventing and alleviating acne by maintaining normal homeostasis (Korean Patent Publication No. 1999-025848).

Thus, the present inventors were working to find a material that is excellent in preventing skin damage caused by ultraviolet rays. When quercetin, genistein and alpha-lipoic acid are mixed, they are effective in preventing skin damage caused by ultraviolet rays. Was excellent, and the present invention was completed by confirming that any one of these components can exhibit a remarkably excellent effect compared to the case of using alone.

An object of the present invention is to provide a composition for preventing skin light damage containing quercetin, genistein and alpha lipoic acid as active ingredients.

In order to achieve the above object, the present invention provides a cosmetic composition for preventing and improving skin damage caused by ultraviolet rays containing quercetin, genistein and alpha lipoic acid as active ingredients.

In addition, the present invention provides a skin external preparation for preventing and improving skin damage caused by ultraviolet rays containing quercetin, genistein and alpha lipoic acid as active ingredients.

In addition, the present invention provides a quasi-drug for preventing and improving skin damage caused by ultraviolet rays containing quercetin, genistein and alpha lipoic acid as active ingredients.

The composition containing quercetin, genistein and alpha lipoic acid according to the present invention as an active ingredient suppresses cytotoxicity by ultraviolet irradiation and exhibits a synergistic effect on alleviating skin oxidative damage caused by light damage on the skin, thereby providing excellent light damage preventing effect. Shows.

Specifically, the composition containing quercetin, genistein and alpha lipoic acid according to the present invention as an active ingredient can suppress the decrease in the survival rate of keratinocytes induced by ultraviolet irradiation, and lipid, a product of oxidative skin damage induced by ultraviolet irradiation By reducing the amount of peroxidation products (TBARS), it shows an excellent effect on alleviating skin damage caused by light damage on the skin.

In particular, the composite composition of quercetin, genistein, and alphalipoic acid according to the present invention suppresses the decrease in keratinocyte survival rate caused by ultraviolet light compared to the single treatment of quercetin, genistein, and alphalipoic acid, or a combination treatment of two or more of these components. It has a remarkably excellent effect by synergistically in the effect and the effect of reducing the amount of lipid peroxide, TBARS.

Hereinafter, the present invention will be described in detail.

The present invention provides a cosmetic composition for preventing and improving skin damage caused by ultraviolet rays containing quercetin, genistein and alpha lipoic acid as active ingredients.

The quercetin, genistein and alpha lipoic acid are preferably included in a ratio of 1: 2: 1: 2: 1 ~ 10, and more preferably 1: 1: 1-5.

The quercetin, genistein and alpha lipoic acid are preferably included in an amount of 0.001 to 10% by weight, respectively, based on the total weight of the total cosmetic composition, and when it is less than 0.001% by weight, the effect is negligible. Problems such as solubility in the formulation may occur.

The cosmetic composition may be added with the quercetin, genistein and alpha lipoic acid as it is, or may be used together with other similarly active ingredients, and may be suitably used according to conventional methods. The mixing amount of the active ingredient can be appropriately determined depending on the intended use.

The cosmetic composition is a solution, gel, solid or dough anhydrous product, emulsion obtained by dispersing an oil phase in an aqueous phase, suspension, microemulsion, microcapsule, microgranules or ionic (liposomes), nonionic vesicle dispersant, cream , Skin, lotion, powder, ointment, essence, spray or conceal stick. It can also be prepared in the form of a foam or aerosol composition further containing a compressed propellant.

In order to use the cosmetic composition as a cosmetic composition for suppressing skin damage caused by ultraviolet rays and protecting the skin in each formulation, various cosmetically acceptable ingredients may be arbitrarily selected and blended according to the formulation or purpose of use. The mixing amount of the active ingredient may be appropriately determined depending on the intended use, and may include, for example, thickening agents, stabilizers, solubilizing agents, conventional adjuvants such as vitamins, pigments and flavoring agents and carriers.

In one embodiment of the present invention, in addition to quercetin, genistein, and alpha lipoic acid, ethanol, polysorbate 80, allantoin, glycerin, tyrphenine, lavender oil, and purified water were added, and these additional components were appropriately added to some or all of them. Can be used.

In addition, the present invention provides a skin external preparation for preventing and improving skin damage caused by ultraviolet rays containing quercetin, genistein and alpha lipoic acid as active ingredients.

The quercetin, genistein and alpha lipoic acid are preferably included at a ratio of 1: 2: 1: 2: 1 ~ 10, and more preferably 1: 1: 1 ~ 5.

The quercetin, genistein and alpha lipoic acid preferably contain 0.001 to 10% by weight, respectively, based on the total weight of the total composition, and when it is less than 0.001% by weight, the effect is minimal, and when it exceeds 10% by weight, skin side effects and formulations Problems such as solubility may occur.

The external preparation for skin is not particularly limited in its formulation, such as the form of a skin coating agent, liquid formulation, powder formulation, capsule, tablet, syrup, etc., and is used in various formulations known in the art as exhibiting a skin protection effect by ultraviolet rays. It is possible.

The external preparation for skin may be formulated to include one or more pharmaceutically acceptable carriers in addition to the active ingredients described above.

In addition, the present invention provides a quasi-drug for preventing and improving skin damage caused by ultraviolet rays containing quercetin, genistein and alpha lipoic acid as active ingredients.

The quercetin, genistein and alpha lipoic acid are preferably included at a ratio of 1: 2: 1: 2: 1 ~ 10, and more preferably 1: 1: 1 ~ 5.

The quercetin, genistein and alpha lipoic acid preferably contain 0.001 to 10% by weight, respectively, based on the total weight of the total composition, and when it is less than 0.001% by weight, the effect is minimal, and when it exceeds 10% by weight, skin side effects and formulations Problems such as solubility may occur.

The quasi-drug is not particularly limited in its formulation, and may be variously formulated in the form of a quasi-drug known in the art as exhibiting a skin protection effect by ultraviolet rays. The formulated products include shampoo, rinse, body wash, hand cleaner, detergent soap, disinfectant cleaner, wipes, mask, patch or filter filler, and include all quasi-drugs in the usual sense.

The quasi-drug may be formulated by including one or more acceptable carriers as a component of the quasi-drug in addition to the active ingredients described above.

Hereinafter, the present invention will be described in detail by the following examples and experimental examples.

However, the following examples and experimental examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following examples and experimental examples.

< Experimental Example  1> Quercetin , Genistein  And Alpha lipoic acid  Effect of UV irradiation on keratinocytes on cellular activity

The effect of quercetin, genistein and alpha lipoic acid on the cell activity of keratinocytes irradiated with ultraviolet light was observed by measuring the cell activity after ultraviolet irradiation by MTT analysis.

 The keratinocytes (HaCat keratinocyte) used in this experiment were purchased from the American Type Culture Collection and used for quercetin (Q4951), Genistein (G6776) and alpha lipoic acid. : T5625) was purchased from Sigma-Aldrich.

 For keratinocytes, Dulbecco's Modified Eagle Medium (DMEM) medium (Gibco BRL NY. USA) containing 10% fetal calf serum, streptomycin (100 U/mL), and penicillin (100 U/mL) was used, and at 37°C. Cells were cultured in a maintained CO2 incubator.

 Quercetin, genistein and alphalipoic acid were dissolved in DMSO (dimethylsulfoxide), diluted in PBS (phosphate buffered saline) and added to the culture.

After putting 1 x 10 ⁴ keratinocytes into each well of a cell culture container (96 well plate) and incubating for 24 hours, quercetin (20 μM/mL), genistein (20 μM/mL) and alpha lipoic acid (100 μM/mL) was added and incubated for 1 hour, followed by irradiation with 40 mJ/Cm ² using a UV irradiator (Philips TL/12RS 40 W: Philips Medical Systems, Netherlands), followed by further incubation for 20 hours. After the cell activity was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide) method. After incubating for 4 hours by adding MTT to a final concentration of 1 mM in the cell culture solution, the culture solution is removed, washed 3 times with HBSS (Hank's balanced salt solution), and then the MTT solution [50% N,N-dimethylformamide ( v/v); 20% sodium dodecyl sulfate (w/v), pH 4.7] The absorbance was measured at 540 nm with an ELISA plate reader (TECAN. Mannedorf, Switzerland) by adding 200 μL. The cell viability was calculated by calculating the absorbance measured by the following equation and was expressed as a comparative cell activity.

Relative vitality= (Experimental absorbance / Control absorbance) × 100

The results are shown in Table 1 below (Table 1).

Prevention of cell damage by UV irradiation of quercetin, genistein and alpha lipoic acid in keratinocytes (HaCat keratinocyte) Experimental group Additives and capacity  Comparative cell activity (%) Normal control - 100 UV irradiation
(UVB 40 mJ/Cm ² )
Control - 45.5 ±4.3  Quercetin County Quercetin (20 μM/mL) 47.2 ±5.6  Genistein Genistein (20 μM/mL) 53.3 ±5.9  Alpha lipoic acid  Alpha lipoic acid (100 μM/mL) 49.5 ±5.3 Quercetin + Genistein Quercetin (20 μM/mL)
Genistein (20 μM/mL) 61.7 ±6.1 Quercetin+Alphafolic Acid Quercetin (20 μM/mL)
Alpha lipoic acid (100 μM/mL) 55.9 ±4.5 Genistein + Alfaic Acid Genistein (20 μM/mL)
Alpha lipoic acid (100 μM/mL) 60.2 ±5.3 Quercetin + Genistein + Alpha Lipoic Acid Quercetin (20 μM/mL)
Genistein (20 μM/mL)
Alpha lipoic acid (100 μM/mL) 76.8 ±6.2

HaCat keratinocyte is a human-derived keratinocyte, and when the cells are damaged by irradiating ultraviolet rays, the cell activity is reduced.

Comparing cell viability is the cell viability of the non-irradiated with ultraviolet rays the control group as a value indicated by a 100% irradiated by ultraviolet ray (UVB 40 mJ / Cm ²⁾ Comparative cell activity of Group (UV control group) will show up as a 45.5% by UV irradiation It was found that the cell activity was reduced and the cell damage appeared.

The comparative cell activity of the quercetin group was 47.2%, and there was no difference from the comparative cell activity of the ultraviolet control group 45.5%, so there was no difference in cell damage between the quercetin group and the ultraviolet control group.

The comparative cell activity of the genistein group was 53.3%, and there was no difference from the comparative cell activity of 45.% of the UV control group, so there was no difference in cell damage between the genistein group and the UV control group.

The comparative cell activity of the alpha lipoic acid group was 49.5%, and there was no difference from the comparative cell activity of the ultraviolet control group 45.5%, so there was no difference in cell damage between the alpha lipoic acid group and the ultraviolet control group.

The comparative cell activity of the quercetin + genistein group was 61.7%, and the comparative cell activity of the ultraviolet control group was increased from 45.5%, and it was found that the quercetin + genistein group had a reduced cell damage than the ultraviolet control group.

The comparative cell activity of the quercetin+alphalipoic acid group was 55.9%, and the comparative cell activity of the ultraviolet control group was increased from 45.5%, and it was found that the quercetin+alpha lipoic acid group had a reduced cell damage than the ultraviolet control group.

The comparative cell activity of the genistein+alphalipoic acid group was increased to 60.2%, and the comparative cell activity of the ultraviolet control group was increased to 45.5%, indicating that the cell damage was decreased in the genistein+alphalipoic acid group than the ultraviolet control group.

The comparative cell activity of the quercetin + genistein + alfafoic acid group was 76.8%, compared to the ultraviolet control group, the quercetin group, genistein group, alpha lipoic acid group, quercetin + genistein group, quercetin + alipophosphoric acid group, and genistein + alfaphosphoric acid group. Increased, the quercetin + genistein + alfafoic acid group showed the greatest effect of preventing cell damage by ultraviolet irradiation.

< Example  1> Light damage  Prevention Cosmetic  Preparation of the composition

Quercetin 0.2% by weight, genistein 0.2% by weight, alphalipoic acid 0.2% by weight, ethanol 1.7% by weight, polysorbate 80 1.0% by weight, allantoin 1.0% by weight, glycerin 5.0% by weight, tyrphenine 0.01% by weight, lavender oil 0.01% % And purified water were added and adjusted to 100% by weight, followed by mixing to prepare a cosmetic composition.

In Table 2 below, (A) was mixed and stirred for 30 minutes at 70°C with a stirrer, and (B) was added to emulsify uniformly using a homomixer for 30 minutes, and gradually cooled to room temperature to prepare a cosmetic composition. .

< Comparative example  1> Quercetin , Genistein  And Alpha lipoic acid  Do not contain Cosmetic  Preparation of the composition

Quercetin, genistein and alpha lipoic acid were excluded from the preparation method of <Example 1>, and the rest were prepared in the same manner.

< Comparative example  2> Genistein  And Alpha lipoic acid  Do not contain Cosmetic  Preparation of the composition

In the method of <Example 1>, genistein and alpha lipoic acid were excluded, and the rest were prepared in the same manner.

<Comparative Example 3> Preparation of a cosmetic composition containing no quercetin and alpha lipoic acid

In the preparation method of <Example 1>, quercetin and alpha lipoic acid were excluded, and the rest were prepared in the same manner.

<Comparative Example 4> Preparation of a cosmetic composition containing no quercetin and genistein

Quercetin and genistein were excluded from the production method of <Example 1>, and the rest were prepared in the same manner.

<Comparative Example 5> Preparation of cosmetic composition containing no alpha lipoic acid

In the preparation method of <Example 1>, alpha lipoic acid was excluded, and the rest were prepared in the same manner.

<Comparative Example 6> Preparation of a cosmetic composition containing no genistein

In the method of <Example 1>, genistein was excluded, and the rest were prepared in the same manner.

<Comparative Example 7> Preparation of a cosmetic composition containing no quercetin

In the method of <Example 1>, genistein was excluded, and the rest were prepared in the same manner.

Lotion ingredient list  ingredient Comparative example
One
(weight%) Comparative example
2
(weight%) Comparative example
3
(weight%) Comparative example
4
(weight%) Comparative example
5
(weight%) Comparative example
6
(weight%) Comparative example
7
(weight%) Example
One
(weight%) end
Quercetin -  0.20 -   -  0.20  0.20  0.20 Genistein - -  0.20   -  0.20   -  0.20  0.20 Alpha lipoic acid - -   -   0.20   -  0.20  0.20  0.20 ethanol  2.30  2.10  2.10  2.10  1.90  2.10  1.90  1.70 Polysorbate 80  1.00  1.00  1.00  1.00  1.00  1.00  1.00  1.00 Allantoin  1.00  1.00  1.00  1.00  1.00  1.00  1.00  1.00 glycerin  5.00  5.00  5.00  5.00  5.00  5.00  5.00  5.00 I Turpinin  0.01  0.01  0.01  0.01  0.01  0.01  0.01  0.01 Lavender oil  0.01  0.01  0.01  0.01  0.01  0.01  0.01  0.01 Purified water  to 100  to 100  to 100  to 100  to 100  to 100  to 100  to 100

< Experimental Example  2> Quercetin , Genistein  And Alphalipoic  Effect of the mixture on skin lipid peroxide levels in UV-irradiated mice

After adapting the mice (hairless mouse, HRS/J, 25-30g male) to the breeding environment, the amount of 120 mJ/Cm ^{2 was} used on the 2nd day using a UV irradiator (Philips TL/12RS 40 W: Philips Medical Systems, Netherlands). It was investigated three times, once. The above-mentioned <Example 1> and <Comparative Examples 1 to 7> each of the cosmetic water prepared in each of the mice 0.4 mL capacity (0.4 mL / mouse) to the back of the mouse 1 hour before and 4 hours after UV irradiation for a total of 6 times each Applied. Five mice were irradiated with UV light for each lotion, and ileum water was applied. After the final irradiation of UV light on the experimental animal, after 48 hours (6 days after the start of the experiment), mice were sacrificed to excise the skin irradiated with UV light to measure the amount of lipid peroxide used as an indicator of oxidative cell damage.

Skin tissue lipid peroxidation was measured by the amount of TBARS (thiobarbituric acid reactive substance). The skin tissue was excised and added to a 10-fold tissue weight (W/V) of 0.1M phosphate buffer (pH 7.4), homogenized with a polytrone homogenizer, and used as a sample for measuring TBARS amount. The amount of TBARS was measured according to the manufacturer's measuring method using the OxiSelect™ TBARS Assay Kit (Goma Biotech Co., Ltd. Seoul Korea).

The results are shown in Table 3 below (Table 3).

The amount of lipid peroxide (TBARS) in mouse skin tissue Experimental group TBARS
(μM/g wet tissue) UV irradiation Control 127.05 ± 21.54 Ultraviolet irradiation
(UVB 100 mJ/Cm ² )
Comparative Example 1 312.31 ± 48.20 Comparative Example 2 298.23 ± 41.92 Comparative Example 3 302.14 ± 45.12 Comparative Example 4 281.89 ± 46.80 Comparative Example 5 252.13 ± 36.53 Comparative Example 6 249.21 ± 31.32 Comparative Example 7 237.17 ± 30.11 Example 1 178.64 ± 25.87

Free radical generation by ultraviolet irradiation is an important factor in cell damage, and lipid peroxide content is a substance used as an indicator of cell damage due to free radicals, and TBARS is widely used as a method for measuring lipid peroxide.

The amount of TBARS in the skin tissue of the ultraviolet irradiation group coated with the cosmetic composition (Comparative Example 1) containing no quercetin, genistein and alpha lipoic acid was increased by 145% compared to the control group not irradiated with ultraviolet light, and oxidized to the skin tissue by ultraviolet irradiation. Enemy damage was found to increase.

The amount of TBARS in the skin tissue of the UV irradiation group to which the cosmetic composition containing quercetin (Comparative Example 2) was applied was not different from the UV irradiation group to which Comparative Example 1 was applied.

The amount of TBARS in the skin tissue of the UV irradiation group to which the cosmetic composition containing genistein (Comparative Example 3) was applied was not different from the UV irradiation group to which Comparative Example 1 was applied.

The amount of TBARS in the skin tissue of the UV irradiation group coated with the cosmetic composition containing alpyrifoic acid (Comparative Example 4) was not different from the UV irradiation group coated with Comparative Example 1.

It was found that the amount of TBARS in the skin tissue of the ultraviolet irradiation group coated with the cosmetic composition containing quercetin and genistein (Comparative Example 5) was reduced by 19% compared to the ultraviolet irradiation group coated with Comparative Example 1, thereby reducing oxidative damage.

It was found that the amount of TBARS in the skin tissue of the UV irradiation group coated with the cosmetic composition containing quercetin and alpha lipoic acid (Comparative Example 6) was reduced by 20% compared to the UV irradiation group applied with Comparative Example 1 to reduce oxidative damage.

The skin tissue TBARS amount of the UV irradiation group coated with the cosmetic composition containing genistein and alpha lipoic acid (Comparative Example 7) was reduced by 24% compared to the UV irradiation group coated with Comparative Example 1, and the oxidative damage was reduced.

The amount of skin tissue TBARS in the ultraviolet irradiation group coated with the cosmetic composition containing quercetin, genistein, and alpha lipoic acid (Example 1) was reduced by 42% compared to the ultraviolet irradiation group coated with Comparative Example 1, Comparative Example 2, Comparative Example 3, compared to Comparative Example 4, Comparative Example 5, Comparative Example 6 and Comparative Example 7, the skin tissue TBARS amount was reduced, it was found that the oxidative damage of the skin tissue is reduced than these.

Claims (5)

A cosmetic composition for preventing and improving skin damage caused by ultraviolet rays containing quercetin, genistein and alpha lipoic acid as active ingredients.
According to claim 1, wherein the quercetin, genistein and alpha lipoic acid is 1 to 2: 1 to 2: 1: 1 to 10 cosmetic composition for preventing and improving skin damage caused by ultraviolet rays, characterized in that it comprises.
The method according to claim 1, further comprising any one or more selected from the group consisting of ethanol, polysorbate 80, allantoin, glycerin, tyrphenine, lavender oil, and purified water. Cosmetic composition.
A skin external preparation for preventing and improving skin damage caused by ultraviolet rays containing quercetin, genistein and alpha lipoic acid as active ingredients.
A quasi-drug for the prevention and improvement of skin damage caused by UV rays containing quercetin, genistein and alpha lipoic acid as active ingredients.