KR20200060070A - Human alphacoronavirus universal primer sets for whole genome amplification method and diagnosis kit - Google Patents

Human alphacoronavirus universal primer sets for whole genome amplification method and diagnosis kit Download PDF

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KR20200060070A
KR20200060070A KR1020180145560A KR20180145560A KR20200060070A KR 20200060070 A KR20200060070 A KR 20200060070A KR 1020180145560 A KR1020180145560 A KR 1020180145560A KR 20180145560 A KR20180145560 A KR 20180145560A KR 20200060070 A KR20200060070 A KR 20200060070A
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Abstract

The present invention relates to a method for obtaining a whole genome sequence for determining the presence of infection by coronavirus, and a use thereof. A method, a kit and a composition of the present invention comprise a set of 11 universal primers capable of amplifying the genomic sequence of coronavirus, thereby enabling easy and rapid identification of the presence of a target virus in a sample and related diseases. In addition, through whole genome sequencing, mutations of novel coronavirus can be identified so as to be effectively used in the identification of novel viruses.

Description

인간 알파코로나바이러스 전장유전체 증폭을 통한 진단키트 및 전장 유전체 서열 확인 방법{Human alphacoronavirus universal primer sets for whole genome amplification method and diagnosis kit} Human alphacoronavirus universal primer sets for whole genome amplification method and diagnosis kit through human alpha coronavirus full-length genome amplification

본 발명은 인간 알파코로나바이러스의 전장유전체를 증폭할 수 있는 프라이머 세트를 활용하여 미량의 시료에서 바이러스 유전체를 증폭하고 염기서열을 분석하여 전장 유전체 정보를 얻어내는 기술에 관한 것이다.The present invention relates to a technique for amplifying a viral genome in a trace amount of samples and analyzing the sequencing to obtain full-length genomic information by utilizing a primer set capable of amplifying the full-length dielectric of human alpha coronavirus.

코로나바이러스(Coronavirus)는 30kb의 positive-sense single-stranded RNA genome을 갖고 있고 있으며 이는 RNA virus 중 가장 크다. 알파코로나바이러스는 코로나바이러의 일종으로 spherical morphology를 가지며, 120-160nm의 capsid를 가지고 있다. 알파코로나바이러스는 인간에게 호흡기 감염을 일으키는 인간 감염 바이러스와 돼지, 고양이, 박쥐 등에서는 호흡 계 감염뿐 아니라 장 감염을 유발하는 동물 감염 바이러스 등이 알려져 있다. 알파코로나 중 인체 감염 종으로는 229E와 NL63 두 종이 있고, 현재 229E의 complete genome과 partial genome이 각 22건 및 5건씩 등록되어 있으며, NL63은 총 45건의 complete genome이 NCBI에 등록되어 있다. Coronavirus has a 30kb positive-sense single-stranded RNA genome, which is the largest RNA virus. Alpha coronavirus is a type of coronavirus, has spherical morphology, and has a capsid of 120-160 nm. Alpha coronavirus is a human infection virus that causes respiratory infections in humans, and animal infection viruses that cause intestinal infections as well as respiratory system infections in pigs, cats, and bats are known. There are 229E and NL63 species of human infections among the alpha corona, and 22 and 5 complete genomes and 2 partial genomes of 229E are registered respectively, and 45 complete genomes of NL63 are registered in NCBI.

2001년 vaccinia virus를 cloning vector로 하여 1973-deposited laboratory-adapted prototype strain of HCoV-229E (VR-740)의 HCoV-229E cDNA clone (Inf-1)과 recombinants를 제작하여 세포에서 증식시킨 뒤 RNA를 추출하고 RT-PCR을 수행하여 complete genome을 추출하였다(Genbank accession: NC_002645). 이후 빠른 분석을 위해 2012년에는 임상샘플에서 HCov-229E total RNA를 추출한 뒤 2001년 시퀀싱 된 genome을 scaffold로 하여 최소 80bp 이상 겹쳐서 합성되는 500bp의 PCR product를 생성하는 bidirectional primer 조합으로 시퀀싱한 complete genome이 보고되었다. In 2001, the vaccinia virus was used as a cloning vector to produce HCoV-229E cDNA clone (Inf-1) and recombinants of the 1973-deposited laboratory-adapted prototype strain of HCoV-229E (VR-740) to proliferate in cells and extract RNA Then, complete genome was extracted by performing RT-PCR (Genbank accession: NC_002645). For rapid analysis, a complete genome sequenced with a bidirectional primer combination that generates a 500-bp PCR product that is synthesized by overlapping at least 80 bp by using a scaffold with a genome sequenced in 2001 after extracting HCov-229E total RNA from a clinical sample in 2012 Was reported.

2017년에는 Haiti의 어린이들의 plasma에서 채취한 HCov-229E를 Vero E6 cell에서 추출하여 Sanger와 Miseq으로 시퀀싱을 수행하였다. HCoV-NL63은 2004년 암스테르담에서 분리되어 Second-strand DNA synthesis를 한 후 restriction enzyme으로 digestion 한 뒤, anchor를 붙이고 primer로 amplify를 하는 cDNA-amplified restriction fragment-length polymorphism technique (VIDISCA : Virus-Discovery-cDNA-AFLP)으로 발견되었고, 475개의 genome fragment를 이용한 complete genome이 보고되었다(Genbank accession: NC_005831). In 2017, HCov-229E collected from plasma of children of Haiti was extracted from Vero E6 cells and sequenced with Sanger and Miseq. HCoV-NL63 was isolated from Amsterdam in 2004, and after second-strand DNA synthesis, digestion with restriction enzyme, followed by anchoring and amplifying with primer, cDNA-amplified restriction fragment-length polymorphism technique (VIDISCA: Virus-Discovery-cDNA-AFLP), and a complete genome using 475 genome fragments was reported (Genbank accession: NC_005831).

덴마크에서는 paediatric patients의 92개의 respiratory specimens로부터 추출한 NL63 RNA를 역전사하고 modified sequence-independent single-primer amplification (SISPA) approach를 이용하여 amplicon을 생성한 후 Hiseq platform으로 시퀀싱을 하여 assembly를 하였다(Genbank accession: JQ765564). In Denmark, NL63 RNA extracted from 92 respiratory specimens from paediatric patients was reverse transcribed and amplicon was generated using a modified sequence-independent single-primer amplification (SISPA) approach, followed by sequencing with Hiseq platform for assembly (Genbank accession: JQ765564 ).

2013년 베타코로나바이러스를 분석하기 위해 기존에 알려진 15개의 베타코로나바이러스를 바탕으로 2.5kb 정도의 15개 amplicon을 만드는 60개의 프라이머 세트를 구성하여 이를 이용하여 reverse transcription을 진행 한 후 NGS로 분석함으로써 기존 방법보다 짧은 시간에 바이러스 전장유전체를 분석하였다. To analyze the beta coronavirus in 2013, based on the 15 beta coronaviruses known previously, 60 sets of primers that make 15 amplicons of about 2.5 kb were constructed, and reverse transcription was performed using this, followed by analysis by NGS. The viral full length was analyzed in a shorter time than the method.

2015년 South Korea에서 Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus를 환자의 객담 샘플에서 바이러스를 추출하여 vero cell로 배양한 후 추출한 RNA로부터 cDNA를 합성하여 평균 300bp 길이의 overlapping PCR primer를 제작하였다. Fragments들은 Illumina MiSeq 50-bp single-end platform으로 시퀀싱되어 complete genome을 얻었다. In 2015, in South Korea, the Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus was extracted from a patient's sputum sample, cultured into a vero cell, and cDNA was synthesized from the extracted RNA to produce an overlapping PCR primer with an average length of 300bp. Fragments were sequenced with the Illumina MiSeq 50-bp single-end platform to obtain a complete genome.

2015년 브라질에서 창궐한 Zika virus는 임산부의 amniotic fluid(양수)에서 채취하여 RNA로부터 double strand cDNA를 합성하고 MiSeq으로 시퀀싱하여 Zika virus genome을 얻었다.Zika virus, which emerged in Brazil in 2015, was collected from amniotic fluid (positive water) of pregnant women, synthesized double strand cDNA from RNA and sequenced with MiSeq to obtain Zika virus genome.

2015년 Sierra Leone, Liberia, Guinea에서 10,000명 이상의 사상자를 낸 Ebola virus의 genome은 38개에서 11개의 primer pair를 이용하여 현장에서 수시간 내에 MinION을 이용하여 시퀀싱하였다. In 2015, the genome of Ebola virus, which caused more than 10,000 casualties in Sierra Leone, Liberia, and Guinea, was sequenced using MinION in the field within a few hours using 38 to 11 primer pairs.

hCoV의 발견은 일찍이 되었지만, 의학적 중요성이 낮고 분리 및 동정의 어려움으로 관심을 받지 못하였다가 2003년 SARS로 진단의 중요성이 커졌다. 그러나 모든 종류의 Human Coronavirus를 검출할 수 있는 sensitive하고 rapid 검출법은 존재하지 않고, monoclonal antibodies를 이용하거나 reverse-transcriptase polymerase chain reaction (RT-PCR)을 이용하여 진단해오고 있다.The discovery of hCoV was early, but its medical significance was low and it was not receiving attention due to difficulties in separation and identification. However, there is no sensitive and rapid detection method capable of detecting all types of human coronavirus, and it has been diagnosed using monoclonal antibodies or reverse-transcriptase polymerase chain reaction (RT-PCR).

알파코로나바이러스에서는 진단을 위해서 2000년대부터 gene 1ab나 gene N을 타켓으로 하는 universal primer를 사용하고자 하였으나, 약 30% 정도만을 정확히 검출하였다. 따라서 각 group별 specific primer를 이용한 diagnosis의 중요성이 대두되었다. For the diagnosis of alpha coronavirus, it was intended to use universal primers targeting gene 1ab or gene N from the 2000s, but only about 30% was accurately detected. Therefore, the importance of diagnosis using specific primers for each group emerged.

1. 대한민국 공개특허공보 제10-2010-0018993호1. Republic of Korea Patent Publication No. 10-2010-0018993

1. Alphacoronavirus. The Springer Index of Viruses: 371-383. doi:10.1007/978-0-387-95919-1_56. Retrieved1. Alphacoronavirus. The Springer Index of Viruses: 371-383. doi: 10.1007 / 978-0-387-95919-1_56. Retrieved 2. Coronavirus Pathogenesis and the Emerging Pathogen Severe Acute Respiratory Syndrome Coronavirus. Microbiol Mol Biol Rev. 2005 Dec; 69(4): 635-6642. Coronavirus Pathogenesis and the Emerging Pathogen Severe Acute Respiratory Syndrome Coronavirus. Microbiol Mol Biol Rev. 2005 Dec; 69 (4): 635-664 3. Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus. J Gen Virol. 2001 Jun;82(Pt 6):1273-813.Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus. J Gen Virol. 2001 Jun; 82 (Pt 6): 1273-81 4. The first complete genome sequences of clinical isolates of human coronavirus 229E. Virus Genes. 2012 Dec;45(3):433-94. The first complete genome sequences of clinical isolates of human coronavirus 229E. Virus Genes. 2012 Dec; 45 (3): 433-9 5. Complete Genome Sequence of Human Coronavirus Strain 229E Isolated from Plasma Collected from a Haitian Child in 2016. Genome Announc. 2017 Nov 22;5(47). pii: e01313-175.Complete Genome Sequence of Human Coronavirus Strain 229E Isolated from Plasma Collected from a Haitian Child in 2016. Genome Announc. 2017 Nov 22; 5 (47). pii: e01313-17 6. Identification of a new human coronavirus. Nat Med. 2004 Apr;10(4):368-736. Identification of a new human coronavirus. Nat Med. 2004 Apr; 10 (4): 368-73 7. Genomic analysis of 16 Colorado human NL63 coronaviruses identifies a new genotype, high sequence diversity in the N-terminal domain of the spike gene and evidence of recombination. J Gen Virol. 2012 Nov;93(Pt 11):2387-987.Genomic analysis of 16 Colorado human NL63 coronaviruses identifies a new genotype, high sequence diversity in the N-terminal domain of the spike gene and evidence of recombination. J Gen Virol. 2012 Nov; 93 (Pt 11): 2387-98 8. Full genome deep sequencing and phylogenetic analysis of novel human betacoronavirus. Emerg Infect Dis. 2013 May;19(5):736-42B8. Full genome deep sequencing and phylogenetic analysis of novel human betacoronavirus. Emerg Infect Dis. 2013 May; 19 (5): 736-42B 9. Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR/KNIH/002_05_2015, Isolated in South Korea. Genome Announc. 2015 Aug 13;3(4). pii: e00787-159.Complete Genome Sequence of Middle East Respiratory Syndrome Coronavirus KOR / KNIH / 002_05_2015, Isolated in South Korea. Genome Announc. 2015 Aug 13; 3 (4). pii: e00787-15 10. Real-time, portable genome sequencing for Ebola surveillance. Nature. 2016 Feb 11;530(7589):228-23210. Real-time, portable genome sequencing for Ebola surveillance. Nature. 2016 Feb 11; 530 (7589): 228-232 11. Genetic variability of human coronavirus OC43-, 229E-, and NL63-like strains and their association with lower respiratory tract infections of hospitalized infants and immunocompromised patients. J Med Virol. 2006 Jul;78(7):938-4911.Genetic variability of human coronavirus OC43-, 229E-, and NL63-like strains and their association with lower respiratory tract infections of hospitalized infants and immunocompromised patients. J Med Virol. 2006 Jul; 78 (7): 938-49

본 발명자들은 NL63과 229E를 포함한 인간 알파코로나 바이러스 및 유사 알파 코로나 바이러스에서 증폭이 가능한 범용 프라이머를 활용하여 미량의 유전체로부터 PCR등을 통하여 증폭하여 증폭된 유전체를 염기서열분석법을 통하여 빠르고 간편하게 바이러스의 전장 유전체 염기서열을 확보하는 방법 및 이를 이용한 진단방법을 개발하여 본 발명을 완성하게 되었다.The present inventors utilize a general-purpose primer that can be amplified in human alpha coronavirus and similar alpha coronavirus, including NL63 and 229E, to amplify the amplified genome through PCR from a small amount of genome through sequencing and quickly and easily through the sequencing method. The present invention was completed by developing a method for securing a genome sequence and a diagnostic method using the same.

본 발명의 목적은 시료 내 알파 코로나 바이러스의 전장 유전체 서열 확인 방법을 제공한다. An object of the present invention is to provide a method for identifying the full-length genome sequence of alpha coronavirus in a sample.

본 발명의 다른 목적은 시료 내 알파 코로나 바이러스의 진단 키트를 제공하는 데 있다.Another object of the present invention is to provide a diagnostic kit for alpha coronavirus in a sample.

본 발명의 또 다른 목적은 알파 코로나 바이러스 유발 질환 검출용 조성물을 제공하는 데 있다. Another object of the present invention is to provide a composition for detecting an alpha corona virus-induced disease.

본 발명의 다른 목적 및 이점은 하기의 발명의 상세한 설명, 청구범위 및 도면에 의해 보다 명확하게 된다.Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

본 발명의 일 양태에 따르면, 본 발명의 다음의 단계를 포함하는 분리된 시료 내 알파 코로나 바이러스의 전장 유전체 서열 확인 방법을 제공한다. (a) 알파 코로나 바이러스 전장 유전체에 상보적인 하나 이상의 정방향 및 역방향 프라이머 세트를 제조하는 단계; (b) 시료에서 RNA를 분리하는 단계; (c) 분리된 RNA를 사용하여 역전사 반응을 수행하는 단계; (d) 역전사 반응이 수행된 시료에 하나 이상의 프라이머 세트를 첨가하여 중합효소 연쇄반응을 실시하는 단계; 및 (e) 차세대 염기서열 분석(Next Generation Seqeuncing, NGS)을 이용한 증폭된 산물의 서열을 분석하는 단계를 포함하는 알파코로나 바이러스의 유전체 서열 확인 방법을 제공한다. According to an aspect of the present invention, there is provided a method for identifying the full-length genome sequence of alpha coronavirus in an isolated sample comprising the following steps of the present invention. (a) preparing one or more forward and reverse primer sets complementary to the alpha corona virus full length genome; (b) isolating RNA from the sample; (c) performing a reverse transcription reaction using the isolated RNA; (d) performing a polymerase chain reaction by adding one or more primer sets to the sample subjected to the reverse transcription reaction; And (e) analyzing the sequence of the amplified product using Next Generation Seqeuncing (NGS).

본 발명에서 상기 프라이머 세트는 서열번호 1 내지 서열번호 20으로 이루어진 군에서 선택된 프라이머 세트를 포함하는 것이 바람직하며, 보다 바람직하게는 상기 프라이머 세트는 서열번호 1 내지 서열번호 20으로 이루어진 10개의 프라이머 세트를 포함하는 것이다. 더불어 상기 (e) 단계는 서열번호 31 내지 87의 프라이머를 사용하는 방법을 제공한다.In the present invention, the primer set preferably includes a primer set selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 20, and more preferably, the primer set comprises 10 primer sets consisting of SEQ ID NO: 1 to SEQ ID NO: 20. To include. In addition, step (e) provides a method using the primers of SEQ ID NOs: 31 to 87.

본 발명에서 상기 시료는 혈액, 혈청, 객담, 소변 또는 생체 조직으로부터 분리된 것일 수 있으나 이에 제한되지는 않는다. In the present invention, the sample may be isolated from blood, serum, sputum, urine, or living tissue, but is not limited thereto.

또한 본 발명에서 상기 알파 코로나 바이러스는 229E 또는 NL63을 포함하는 방법을 제공한다. In addition, in the present invention, the alpha corona virus provides a method comprising 229E or NL63.

본 발명의 다른 양태에 따르면, 본 발명은 알파 코로나 바이러스 전장 유전체에 상보적인 하나 이상의 정방향 및 역방향 프라이머 세트를 포함하는 알파 코로나 바이러스 진단 키트를 제공한다.According to another aspect of the present invention, the present invention provides an alpha corona virus diagnostic kit comprising one or more forward and reverse primer sets complementary to the alpha corona virus full-length genome.

본 발명의 또 다른 양태에 따르면, 본 발명은 알파 코로나 바이러스 전장 유전체에 상보적인 하나 이상의 정방향 및 역방향 프라이머 세트를 포함하는 알파 코로나 바이러스 유발 질환 진단용 조성물을 제공한다.According to another aspect of the present invention, the present invention provides a composition for diagnosing alpha corona virus-induced disease comprising a set of one or more forward and reverse primers complementary to the alpha corona virus full-length genome.

본 발명의 특징 및 이점을 요약하면 다음과 같다:The features and advantages of the present invention are summarized as follows:

(a) 본 발명은 인간 알파 코로나 바이러스 전장 유전체의 서열 획득 방법 및 이의 용도에 관한 것이다.(a) The present invention relates to a method for obtaining sequences of human alpha corona virus full length genome and uses thereof.

(b) 본 발명의 방법, 키트 및 조성물은 인간 알파 코로나 바이러스의 전장 유전체를 증폭시킬 수 있는 정방향 및 역방향 프라이머 세트를 이용하고, 유전체 증폭을 통하기 때문에 미량의 바이러스 시료 및 미배양 임상시료 등에서 인간 알파 코로나 바이러스의 존재 유무 및 변종유무를 간편하게 빠르게 확인할 수 있다. (b) The method, kit, and composition of the present invention uses a set of forward and reverse primers capable of amplifying the full-length genome of human alpha corona virus, and through genome amplification, human alpha in a small amount of viral samples and uncultured clinical samples, etc. The presence or absence of corona virus can be quickly and easily confirmed.

(c) 따라서, 본 발명의 방법, 키트 및 조성물은 인간 알파 코로나 바이러스의 전장 게놈 서열을 매우 정확하고 신속하게 얻을 수 있게 해주며, 이를 통해 인간 알파 코로나 바이러스로 유발된 질환의 진단에 효과적으로 적용될 수 있다.(c) Thus, the methods, kits and compositions of the present invention enable to obtain the full-length genomic sequence of human alpha corona virus very accurately and quickly, and thus can be effectively applied to the diagnosis of diseases caused by human alpha corona virus. have.

도 1은 본 발명을 통한 바이러스 전장 유전체 분석의 개념도로써 프라이머 및 증폭체의 숫자는 도면에 제시된 것으로 제한되는 것은 아니다.
도 2은 본 발명의 실시예를 통한 229E와 NL63을 증폭한 핵산 조각의 전기 영동 분석 결과이다.
도 3은 본 발명의 실시예를 통해 확보된 229E 염기 서열의 계통도로써 확보된 염기서열이 원래 염기서열과 같은 분류에 속함을 보여주는 결과이다.1 is a conceptual diagram of viral full-length genome analysis through the present invention, and the number of primers and amplification products is not limited to those shown in the drawings.
2 is a result of electrophoresis analysis of nucleic acid fragments amplified 229E and NL63 through an embodiment of the present invention.
3 is a result showing that the nucleotide sequence secured as a phylogenetic diagram of the 229E nucleotide sequence obtained through an embodiment of the present invention belongs to the same classification as the original nucleotide sequence.

본 발명의 목적을 달성하기 위하여, 본 발명은In order to achieve the object of the present invention, the present invention

(a) 알파 코로나 바이러스 전장 유전체에 상보적인 하나 이상의 정방향 및 역방향 프라이머 세트를 제조하는 단계; (b) 시료에서 RNA를 분리하는 단계; (c) 분리된 RNA를 사용하여 역전사 반응을 수행하는 단계; (d) 역전사 반응이 수행된 시료에 하나 이상의 프라이머 세트를 첨가하여 중합효소 연쇄반응을 실시하는 단계; 및 (e) 차세대 염기서열 분석(Next Generation Seqeuncing, NGS)을 이용한 증폭된 산물의 서열을 분석하는 단계(a) preparing one or more forward and reverse primer sets complementary to the alpha corona virus full length genome; (b) isolating RNA from the sample; (c) performing a reverse transcription reaction using the isolated RNA; (d) performing a polymerase chain reaction by adding one or more primer sets to the sample subjected to the reverse transcription reaction; And (e) analyzing the sequence of the amplified product using Next Generation Seqeuncing (NGS).

를 포함하는 알파코로나 바이러스의 유전체 서열 확인 방법을 제공한다. It provides a method for identifying the genomic sequence of the alpha corona virus comprising a.

본 발명의 실시예에 따른 방법에 있어서, 상기 (c)단계의 중합효소 연쇄반응 (Polymerase Chain Reaction, PCR)은, 재조합효소 중합효소 증폭(Recombinase Polymerase amplification, RPA)도 가능하다. In the method according to the embodiment of the present invention, the polymerase chain reaction (PCR) of step (c) may also be performed by recombinase polymerase amplification (RPA).

또한 본 발명의 실시예에 따른 방법에 있어서, 상기 (e) 단계의 차세대 염기서열 분석(NGS)은 롱리드 시퀀싱 (Long Read Sequecing) 방법으로 단일 DNA 분자 시퀀싱이고 전류를 염기신호를 읽는 Oxford Nanopore Technologies의 Minion을 사용하였으나, 동일한 롱리드 시퀀싱 방법이며, 단일 DNA 분자 시퀀싱이지만 형광 펄스(Pulse)를 이용한 Pacific Bioscience (Pacbio)의 SMRT (Single molecule real time)도 가능하다.In addition, in the method according to the embodiment of the present invention, the next-generation sequencing (NGS) of step (e) is a single DNA molecule sequencing by Long Read Sequecing method and Oxford Nanopore Technologies that reads the current base signal Using Minion, it is the same long-lead sequencing method, and single DNA molecule sequencing, but single molecule real time (SMRT) of Pacific Bioscience (Pacbio) using fluorescence pulses is also possible.

본 발명의 방법의 전체 개념도는 도1에 나타난 바와 같다. The overall conceptual diagram of the method of the present invention is shown in FIG. 1.

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 실시예에 제한되는 것으로 해석되지는 않는다. Hereinafter, the present invention will be described in more detail through examples. Since these examples are only for illustrating the present invention, it should not be construed that the scope of the present invention is limited to these examples.

<실시예 1> 바이러스 origin<Example 1> Virus origin

1966년에 분리보고된 이 균주의 참조유전체가 존재하지 않으므로, HCoV-229E는 ATCC에서 VR-740을 구입하였고, NL63 RNA는 국가병원체자원은행(NCCP 43214)에서 구입하여 사용하였다.Since the reference genome of this strain reported in 1966 was not present, HCoV-229E purchased VR-740 from ATCC, and NL63 RNA was purchased from the National Pathogen Resource Bank (NCCP 43214).

<실시예 2> 바이러스 세포배양 및 RNA 추출<Example 2> Virus cell culture and RNA extraction

바이러스 세포는 생물안전성 2등급 추출 배양 시설 내 이산화탄소 배양기에서 배양하였고, MRC-5(ATCC CCL171) 및 Caco-2(ATCC HTB-37)을 사용하였다. 바이러스 접종 전 37℃ 에서 MEM 배지를 이용하여 배양하였으며 접종후에는 같은 배지에서 33℃에서 5일간 배양하였다.The virus cells were cultured in a carbon dioxide incubator in a biosafety grade 2 extraction culture facility, and MRC-5 (ATCC CCL171) and Caco-2 (ATCC HTB-37) were used. The cells were cultured using MEM medium at 37 ° C before virus inoculation, and cultured at 33 ° C for 5 days in the same medium after inoculation.

RNA 추출을 위하여 감염된 세포 배양액에서는 QIAmp viral RNA minikit (Qiagen)를 사용하였고, 감염된 세포에서는 RNeasy minikit (Qiagen)를 제조자의 사용설명서에 따라 추출하였다. For RNA extraction, QIAmp viral RNA minikit (Qiagen) was used in the infected cell culture, and RNeasy minikit (Qiagen) was extracted from the infected cells according to the manufacturer's instructions.

<실시예 3> 프라이머 설계 및 선정 기준<Example 3> Primer design and selection criteria

유전체 정렬을 통해 보존된 서열 중심으로 primer 후보군을 표2와 같이 69개 선정하였다. Primer 후보군 가운데 50 ℃ < Tm < 60 ℃이며 degeneracy 가 3개 이하이고 Inosine(N base)가 1개 이하인 primer 가운데 2912-5342bp 사이로 amplicon이 생성되는 총 20개의 primer, 10개 pair를 선정하여 실험에 사용하였다. 선정된 총 20개의 프라이머 세트 (Primer sets)는 표1과 같다. 69 candidate primer groups were selected based on the sequence conserved through genome alignment. Among primer candidate groups, 50 primers <Tm <60 ℃, degeneracy of 3 or less, and inosine (N base) of primers of 2912-5342bp among 20 primers and 10 pairs of amplicon are selected and used in the experiment. Did. Table 20 shows the total of 20 primer sets selected.

프라이머 세트Primer set PrimerPrimer Forward
(5＇-> 3＇)Forward
(5 ＇-> 3＇) Reverse
(5＇-> 3＇)Reverse
(5 ＇-> 3＇) length
(bp)length
(bp) Set 1Set 1 43 (서열번호 1)
(TAGACTTTGTGTCTACTYYTCTC)43 (SEQ ID NO: 1)
(TAGACTTTGTGTCTACTYYTCTC) 5385 (서열번호 2)
(GCATTACCCAACAATTATTT)5385 (SEQ ID NO: 2)
(GCATTACCCAACAATTATTT) 53425342 Set 2Set 2 4358 (서열번호 3)
(TTTAATGTTGTWGGKCCICG)4358 (SEQ ID NO: 3)
(TTTAATGTTGTWGGKCCICG) 9077 (서열번호 4)
(GCATAACAAGCCACGTA)9077 (SEQ ID NO: 4)
(GCATAACAAGCCACGTA) 47194719 Set 3Set 3 8201 (서열번호 5)
(GGTGGTRAYAATGTTTATTGYTA)8201 (SEQ ID NO: 5)
(GGTGGTRAYAATGTTTATTGYTA) 11113 (서열번호 6)
(AGCCAAAATCACTTGTTA)11113 (SEQ ID NO: 6)
(AGCCAAAATCACTTGTTA) 29122912 Set 4Set 4 9077 (서열번호 7)
(TAYCGYTGYGCTTGTTATGC)9077 (SEQ ID NO: 7)
(TAYCGYTGYGCTTGTTATGC) 13477 (서열번호 8)
(GGACACCATCATAAAAC)13477 (SEQ ID NO: 8)
(GGACACCATCATAAAAC) 44004400 Set 5Set 5 12255 (서열번호 9)
(CTGGTARYGGTCARGCTAT)12255 (SEQ ID NO: 9)
(CTGGTARYGGTCARGCTAT) 15343 (서열번호 10)
(CATTAGTCACAACATGG)15343 (SEQ ID NO: 10)
(CATTAGTCACAACATGG) 30883088 Set 6Set 6 13477 (서열번호 11)
(GTKTTTATWGATGGTGTWCC)13477 (SEQ ID NO: 11)
(GTKTTTATWGATGGTGTWCC) 17266 (서열번호 12)
(AACAAACTTGACTCCAGG)17266 (SEQ ID NO: 12)
(AACAAACTTGACTCCAGG) 37893789 Set 7Set 7 15343 (서열번호 13)
(CCKATGTTGTGYACTAARTG)15343 (SEQ ID NO: 13)
(CCKATGTTGTGYACTAARTG) 20047 (서열번호 14)
(CTCCATCTACATATCGA)20047 (SEQ ID NO: 14)
(CTCCATCTACATATCGA) 47044704 Set 8Set 8 17266 (서열번호 15)
(CCTGGYAGTCAYAGTTTGTT)17266 (SEQ ID NO: 15)
(CCTGGYAGTCAYAGTTTGTT) 22457 (서열번호 16)
(TAAGGCTCTTCATGTTTTACA)22457 (SEQ ID NO: 16)
(TAAGGCTCTTCATGTTTTACA) 51915191 Set 9Set 9 19333 (서열번호 17)
(TATGGTGATGTKTCWAAAACTAC)19333 (SEQ ID NO: 17)
(TATGGTGATGTKTCWAAAACTAC) 24066 (서열번호 18)
(TCTAATAAGGAAGTTTAGTTGA)24066 (SEQ ID NO: 18)
(TCTAATAAGGAAGTTTAGTTGA) 47334733 Set 10Set 10 22457 (서열번호 19)
(TGTAAAACYATWGAAGAYGCCTTA)22457 (SEQ ID NO: 19)
(TGTAAAACYATWGAAGAYGCCTTA) polyT (서열번호 20)
(TTTTTTTTTTTTT-GTGTATCCATATC)polyT (SEQ ID NO: 20)
(TTTTTTTTTTTTTTT-GTGTATCCATATC) 50005000

<실시예 4> Reverse Transcription<Example 4> Reverse Transcription

HCoV-229E와 HCoV-NL63 RNA를 Random hexamer와 Superscrip III First-Strand Synthesis SuperMix (Cat No. 18080400) 를 이용하여 Reverse Transcript 반응을 수행하였다. Reverse Transcript reaction was performed using HCoV-229E and HCoV-NL63 RNA using Random hexamer and Superscrip III First-Strand Synthesis SuperMix (Cat No. 18080400).

1μl RNA (conc. 50 ng/ul), 0.5ul random hexamer (5＇ -NNNNNN-3 ＇50ng/ul), 1μl 10mM dNTP mix, 최종 볼륨이 8μl가 되도록 DEPC water를 0.2μl PCR tube에 넣고 가볍게 혼합하였고, 65℃에서 5분간 incubation 한 후 ice에서 최소 1분 이상 식히고 가볍게 centrifugation한 후, 얼음에 tube를 꽂고 10μl 2X first-strand reaction mix, 2μl Superscript III enzyme mix를 넣은 후 가볍게 혼합하였다. Thermal cycler를 이용하여 25℃에서 10분간 incubation 한 후 50℃에서 70분 역전사를 진행한 뒤, 85℃에서 5분간 두어 효소의 불활성화를 진행하였다.1μl RNA (conc. 50 ng / ul), 0.5ul random hexamer (5 ＇-NNNNNN-3＇ 50ng / ul), 1μl 10mM dNTP mix, put DEPC water into 0.2μl PCR tube and lightly mix to make final volume 8μl After incubation at 65 ° C for 5 minutes, cooled for at least 1 minute on ice and centrifuged lightly, then a tube was added to the ice, 10 μl 2X first-strand reaction mix, and 2 μl Superscript III enzyme mix were added and lightly mixed. After incubation at 25 ° C for 10 minutes using a thermal cycler, reverse transcription was performed at 50 ° C for 70 minutes, and then at 85 ° C for 5 minutes, the enzyme was inactivated.

1μl RNase H를 첨가한 후 37℃에서 20분간 incubation하였고, RT 반응을 최적화하기 위하여 다양한 RNA template(50ng, 1ug), random hexamer(1ul, 0.5ul, 0.1ul) 및 RT reaction time(50, 70, 90min) 조건에서 최적화하였다. After adding 1 μl RNase H, incubation was performed at 37 ° C for 20 minutes, and various RNA templates (50 ng, 1 ug), random hexamer (1 ul, 0.5 ul, 0.1 ul) and RT reaction time (50, 70, 90min).

<실시예 5> Polymerase Chain Reaction<Example 5> Polymerase Chain Reaction

설계한 primer set를 이용하여 PCR을 수행하였다. PCR was performed using the designed primer set.

1μl First strand cDNA, 1μl Forward primer (conc. 10 pmol/ul), 1μl Reverse primer (conc. 10 pmol/ul), 25μl KAPA hotstart ready mix (catalog number KR0370), 22μl 멸균증류수를 0.2μl PCR tube에 넣고 혼합하였다. 1μl First strand cDNA, 1μl Forward primer (conc. 10 pmol / ul), 1μl Reverse primer (conc. 10 pmol / ul), 25μl KAPA hotstart ready mix (catalog number KR0370), 22μl sterile distilled water in 0.2μl PCR tube Mixed.

PCR 조건은 94℃ 5분 후, denaturation 94℃ 30초, annealing 55℃ 1분, extension 72℃ 4분으로 30회를 진행하고 final extension 72℃ 10분 동안 수행하였고, QIAquick gel extraction kit (Qiagen, Germantown, MD) 을 이용하여 PCR products를 purified하였으며 동일한 양의 amplicon으로 pooling하였다.PCR conditions were carried out 30 times with 94 ° C 5 minutes, denaturation 94 ° C 30 seconds, annealing 55 ° C 1 minute, extension 72 ° C 4 minutes, and final extension 72 ° C for 10 minutes, QIAquick gel extraction kit (Qiagen, Germantown) , MD) and purified PCR products and pooled with the same amount of amplicon.

PCR 최적화를 위하여 Annealing temperature는 55-60℃ temperature gradient PCR (55, 55.3, 56, 56.9, 58.1, 59, 59.6, 60)을 수행하였고, Extension time은 3, 4, 5분동안 수행하였으며, Number of PCR cycle은 30회, 40회 동안 수행하였다.For PCR optimization, annealing temperature was performed at 55-60 ℃ temperature gradient PCR (55, 55.3, 56, 56.9, 58.1, 59, 59.6, 60), and extension time was performed for 3, 4, and 5 minutes, Number of PCR cycle was performed for 30 times and 40 times.

<실시예 6> Sequencing<Example 6> Sequencing

PCR amplicon은 Oxford nanopore사가 제시한 표준적인 방법에 따라 PCR에 사용된 프라이머를 기반으로 추가로 229E, NL63 확보된 염기서열정보의 끝부분의 서열의 프라이머를 이용하여 primer walking법으로 sequencing을 진행하여 완료하였다. PCR amplicon is completed by sequencing by primer walking method using primers at the end of the sequence information obtained in 229E, NL63 additionally based on primers used for PCR according to the standard method suggested by Oxford nanopore. Did.

Supplement table primer list(229E, NL63)Supplement table primer list (229E, NL63) 서열번호Sequence number NameName Sequence(5＇-> 3＇)Sequence (5 ＇-> 3＇) 2121 Alpha13F Alpha13F TTWTCTATCTAYAGATAGARAATTWTCTATCTAYAGATAGARAA 2222 Alpha41FAlpha41F TTTAGACTTTGTGTCTACTYYTCTCTTTAGACTTTGTGTCTACTYYTCTC 2323 Alpha43FAlpha43F TAGACTTTGTGTCTACTYYTCTCTAGACTTTGTGTCTACTYYTCTC 2424 Alpha62FAlpha62F TCTCAACTAAACGAAATTTTTTCTCAACTAAACGAAATTTTT 2525 Alpha252FAlpha252F AAGCAAMGTTYTGTCKTTGTGGAAGCAAMGTTYTGTCKTTGTGG 2626 Alpha950F Alpha950F GGTTCICCIKTTATKACIAATGGTGGTTCICCIKTTATKACIAATGGT 2727 Alpha1421FAlpha1421F GGTGTYATWGAYATTAGTGGTGTYATWGAYATTAGT 2828 Alpha2981F Alpha2981F GGTGGYAARRTWTCWTTYTCTGAGGTGGYAARRTWTCWTTYTCTGA 2929 Alpha3940F Alpha3940F TRTIGATGGTTTYGGTGTTRTIGATGGTTTYGGTGT 3030 Alpha4358FAlpha4358F TTTAATGTTGTWGGKCCICGTTTAATGTTGTWGGKCCICG 3131 Alpha4904FAlpha4904F TAYATGTGYGTTKTRCCATCTAYATGTGYGTTKTRCCATC 3232 Alpha5385FAlpha5385F AYAATAATTGTTGGGTKAATGCAYAATAATTGTTGGGTKAATGC 3333 Alpha5546FAlpha5546F AAGGGTGATGCTGARGAAAGGGTGATGCTGARGA 3434 Alpha6842FAlpha6842F TATGTTAATGCWAATGGTGGTTATGTTAATGCWAATGGTGGT 3535 Alpha7517FAlpha7517F GCKTGYTGTATGCGYGCGCKTGYTGTATGCGYGC 3636 Alpha8201FAlpha8201F GGTGGTRAYAATGTTTATTGYTAGGTGGTRAYAATGTTTATTGYTA 3737 Alpha8432F Alpha8432F TGGTWTGTTAAYGATGGAMGTGTTGGTWTGTTAAYGATGGAMGTGT 3838 Alpha8933FAlpha8933F GARAGTGCTGCWGCWGGGARAGTGCTGCWGCWGG 3939 Alpha8961FAlpha8961F TWGAYATKCGTTCTTATGATWGAYATKCGTTCTTATGA 4040 Alpha9077FAlpha9077F TAYCGYTGYGCTTGTTATGCTAYCGYTGYGCTTGTTATGC 4141 Alpha9203FAlpha9203F ATGGCRCARCCRTCTGGATGGCRCARCCRTCTGG 4242 Alpha10476F Alpha10476F TGGCGYTTTKCTARAGATGGCGYTTTKCTARAGA 4343 Alpha10814F Alpha10814F AARTAYATGRTWGCTAATGGAARTAYATGRTWGCTAATGG 4444 Alpha11113FAlpha11113F TAARCAYAGTGATTTTGGWCTTAARCAYAGTGATTTTGGWCT 4545 Alpha11189F Alpha11189F TCITCWTTTGTTRGTATGCCTCITCWTTTGTTRGTATGCC 4646 Alpha11360FAlpha11360F AGRATGGCTGAACAAGCTGCAGRATGGCTGAACAAGCTGC 4747 Alpha11507FAlpha11507F GCACGTRATGGTGTTGTGCACGTRATGGTGTTGT 4848 Alpha11624FAlpha11624F TAYGCKGGIGTTGTTTGGACTAYGCKGGIGTTGTTTGGAC 4949 Alpha12179FAlpha12179F GCTGTTARACAWGGYGCAAAACCGCTGTTARACAWGGYGCAAAACC 5050 Alpha12255FAlpha12255F CTGGTARYGGTCARGCTATCTGGTARYGGTCARGCTAT 5151 Alpha12532FAlpha12532F TCTAGTGCMGCTCGACTAGATCTAGTGCMGCTCGACTAGA 5252 Alpha12717FAlpha12717F GGAMCACGAGCARTCCATGTAGGAMCACGAGCARTCCATGTA 5353 Alpha13477FAlpha13477F GTKTTTATWGATGGTGTWCCGTKTTTATWGATGGTGTWCC 5454 Alpha13816FAlpha13816F GGTGATGCTGCWRTTAAAGATTTTGAGGTGATGCTGCWRTTAAAGATTTTGA 5555 Alpha13868FAlpha13868F CTTAATAARAGYGCTGGKTGGCCCTTAATAARAGYGCTGGKTGGCC 5656 Alpha14036FAlpha14036F CARAAIGGTGATGCTGCCARAAIGGTGATGCTGC 5757 Alpha14220FAlpha14220F CTTAATAARAGYGCTGKTTGGCCCTTAATAARAGYGCTGKTTGGCC 5858 Alpha14742FAlpha14742F GGTACRACTTCWGGTGAYGCGGTACRACTTCWGGTGAYGC 5959 Alpha15343FAlpha15343F CCKATGTTGTGYACTAARTGCCKATGTTGTGYACTAARTG 6060 Alpha15532FAlpha15532F GCTGGWAATRTITTTGGTTTGCTGGWAATRTITTTGGTTT 6161 Alpha16114FAlpha16114F ACAATACAGGGTCCICCTGGTAGTGGACAATACAGGGTCCICCTGGTAGTGG 6262 Alpha16561F Alpha16561F CGWATGTGTGCWATAGGMCCCGWATGTGTGCWATAGGMCC 6363 Alpha17266F Alpha17266F CCTGGYAGTCAYAGTTTGTTCCTGGYAGTCAYAGTTTGTT 6464 Alpha17320F Alpha17320F GGTTGGTTRGGWATGGATGTGGTTGGTTRGGWATGGATGT 6565 Alpha17989F Alpha17989F TTTAAACAYGCWTTGGGITGTGATTTAAACAYGCWTTGGGITGTGA 6666 Alpha18574F Alpha18574F TTTGAWKKTTATAATTTGTGGCATTTGAWKKTTATAATTTGTGGCA 6767 Alpha18874F Alpha18874F TTRTGGGATTATGAAGCWGATTRTGGGATTATGAAGCWGA 6868 Alpha19333F Alpha19333F TATGGTGATGTKTCWAAAACTACTATGGTGATGTKTCWAAAACTAC 6969 Alpha19735F Alpha19735F TGTAATTTRTATAATTAYGGTGCTGTAATTTRTATAATTAYGGTGC 7070 Alpha20047F Alpha20047F TCWGATATGTAYGATGGYAGTCWGATATGTAYGATGGYAG 7171 Alpha20829F01 Alpha20829F01 TGYTTATGGTCTGTTTCTGGTGYTTATGGTCTGTTTCTGG 7272 Alpha20829F02 Alpha20829F02 TGYTTATGGCCTGTACCTGGTGYTTATGGCCTGTACCTGG 7373 Alpha20829F03 Alpha20829F03 TGYYTCIGGCCTGTWCCTGGTGYYTCIGGCCTGTWCCTGG 7474 Alpha20846F01 Alpha20846F01 GGTTTTGTYTATTTTAAYGGTACTGGGGTTTTGTYTATTTTAAYGGTACTGG 7575 Alpha20846F02 Alpha20846F02 GGTTTTGTYTATTTTAAYGCCACTGGGGTTTTGTYTATTTTAAYGCCACTGG 7676 Alpha22436F Alpha22436F CTYAAGCAGTAYACTTCTGCTTGCTYAAGCAGTAYACTTCTGCTTG 7777 Alpha22457F Alpha22457F TGTAAAACYATWGAAGAYGCCTTATGTAAAACYATWGAAGAYGCCTTA 7878 Alpha22789F Alpha22789F TGATGCWGARCGWATGGCTGATGCWGARCGWATGGC 7979 Alpha23909F Alpha23909F TGGCCITGGTGGGTKTGGTGGCCITGGTGGGTKTGG 8080 Alpha24066F Alpha24066F TCAACTAAACTTCCTTATTAYGATCAACTAAACTTCCTTATTAYGA 8181 Alpha25053F Alpha25053F GTTGGAATITWATYCTAACGTTGGAATITWATYCTAAC 8282 Alpha25287F01 Alpha25287F01 GTTTCAGACTTTTCCGWCGTGGTTTCAGACTTTTCCGWCGTG 8383 Alpha25287F02 Alpha25287F02 GTTTCAGACTTTGGCGCCGTGGTTTCAGACTTTGGCGCCGTG 8484 Alpha25679F Alpha25679F TTGCTTCATTTIWTCTAAACTRAACTTGCTTCATTTIWTCTAAACTRAAC 8585 Alpha25980F Alpha25980F GGTGTYGTYTGGGTTGCTGGTGTYGTYTGGGTTGCT 8686 Alpha27065F Alpha27065F TAGTCTTAYACACAATGGTARGCCTAGTCTTAYACACAATGGTARGCC 8787 Alpha27168F Alpha27168F TGGAGTAMTYAAAGATCGCTGGAGTAMTYAAAGATCGC

<실시예 7> Assembly<Example 7> Assembly

Minion을 통해서 얻어진 염기서열은 bowtie2를 이용하여 알려진 유전체 정보를 참조로 하여 assembly하였다. Assembly된 유전체 염기서열은 참조서열 대비 99.88%의 일치도를 나타내었으며 염기서열은 다음과 같다.The base sequence obtained through Minion was assembled using reference to known genomic information using bowtie2. The assembled genome sequence showed 99.88% identity compared to the reference sequence, and the base sequence is as follows.

>1 229E|NC_002645:1> 1 229E | NC_002645: 1

ACTTAAGTACCTTATCTATCTACAGATAGAAAAGTTGCTTTTTAGACTTTGTGTCTACTTACTTAAGTACCTTATCTATCTACAGATAGAAAAGTTGCTTTTTAGACTTTGTGTCTACTT

TTCTCAACTAAACGAAATTTTTGCTATGGCCGGCATCTTTGATGCTGGAGTCGTAGTGTATTCTCAACTAAACGAAATTTTTGCTATGGCCGGCATCTTTGATGCTGGAGTCGTAGTGTA

ATTGAAATTTCATTTGGGTTGCAACAGTTTGGAAGCAAGTGCTGTGTGTCCTAGTCTAAGATTGAAATTTCATTTGGGTTGCAACAGTTTGGAAGCAAGTGCTGTGTGTCCTAGTCTAAG

GGTTTCGTGTTCCGTCACGAGATTCCATTCTACAAACGCCTTACTCGAGGTTCCGTCTCGGGTTTCGTGTTCCGTCACGAGATTCCATTCTACAAACGCCTTACTCGAGGTTCCGTCTCG

TGTTTGTGTGGAAGCAAAGTTCTGTCTTTGTGGAAACCAGTAACTGTTCCTAATGGCCTGTGTTTGTGTGGAAGCAAAGTTCTGTCTTTGTGGAAACCAGTAACTGTTCCTAATGGCCTG

CAACTGTGTGACACTTGCCGTAGCAAGTGATTCTGAAATTTCTGCAAATGGCTGTTCTACCAACTGTGTGACACTTGCCGTAGCAAGTGATTCTGAAATTTCTGCAAATGGCTGTTCTAC

TATTGCGCAAGCCGTCCGCCGTTATAGCGAGGCCGCTAGCAATGGTTTTAGGGCATGCCGTATTGCGCAAGCCGTCCGCCGTTATAGCGAGGCCGCTAGCAATGGTTTTAGGGCATGCCG

ATTTGTTTCATTAGATTTGCAGGATTGCATCGTTGGCATTGCAGACGATACATATGTTATATTTGTTTCATTAGATTTGCAGGATTGCATCGTTGGCATTGCAGACGATACATATGTTAT

GGGTCTGCATGGCAATCAGACGTTGTTTTGCAACATAATGAAATTTTCTGACCGTCCTTTGGGTCTGCATGGCAATCAGACGTTGTTTTGCAACATAATGAAATTTTCTGACCGTCCTTT

TATGCTTCATGGGTGGTTGGTTTTTTCCAATTCAAATTACCTTTTGGAGGAATTTGATGTTATGCTTCATGGGTGGTTGGTTTTTTCCAATTCAAATTACCTTTTGGAGGAATTTGATGT

TGTCTTCGGTAAGAGAGGTGGTGGTAATGTGACATACACTGACCAGTATCTCTGTGGCGCTGTCTTCGGTAAGAGAGGTGGTGGTAATGTGACATACACTGACCAGTATCTCTGTGGCGC

CGATGGCAAACCTGTTATGAGTGAAGATTTATGGCAGTTTGTTGACCATTTCGGTGAGAACGATGGCAAACCTGTTATGAGTGAAGATTTATGGCAGTTTGTTGACCATTTCGGTGAGAA

CGAAGAAATTATCATCAATGGTCATACTTACGTTTGTGCTTGGCTTACTAAGCGTAAGCCCGAAGAAATTATCATCAATGGTCATACTTACGTTTGTGCTTGGCTTACTAAGCGTAAGCC

CTTAGATTACAAACGTCAGAACAACCTTGCCATTGAAGAGATTGAATATGTGCATGGTGACTTAGATTACAAACGTCAGAACAACCTTGCCATTGAAGAGATTGAATATGTGCATGGTGA

TGCTTTGCATACACTACGCAATGGTTCTGTTCTTGAAATGGCTAAGGAAGTGAAGACATCTGCTTTGCATACACTACGCAATGGTTCTGTTCTTGAAATGGCTAAGGAAGTGAAGACATC

TAGTAAAGTTGTGTTAAGCGATGCTCTTGACAAACTTTACAAAGTCTTTGGTTCTCCTGTTAGTAAAGTTGTGTTAAGCGATGCTCTTGACAAACTTTACAAAGTCTTTGGTTCTCCTGT

TATGACAAATGGTTCCAACATCCTAGAGGCCTTTACTAAACCTGTGTTTATTAGTGCATTTATGACAAATGGTTCCAACATCCTAGAGGCCTTTACTAAACCTGTGTTTATTAGTGCATT

AGTTCAATGTACTTGTGGTACCAAGTCTTGGTCTGTTGGTGATTGGACCGGTTTTAAATCAGTTCAATGTACTTGTGGTACCAAGTCTTGGTCTGTTGGTGATTGGACCGGTTTTAAATC

CTCTTGTTGCAACGTGATCAGTAATAAACTGTGTGTTGTTCCCGGTAATGTTAAACCTGGCTCTTGTTGCAACGTGATCAGTAATAAACTGTGTGTTGTTCCCGGTAATGTTAAACCTGG

TGATGCTGTGATTACCACTCAGCAAGCTGGTGCTGGTATTAAGTATTTTTGTGGCATGACTGATGCTGTGATTACCACTCAGCAAGCTGGTGCTGGTATTAAGTATTTTTGTGGCATGAC

TCTTAAGTTTGTTGCAAATATTGAAGGTGTCTCTGTTTGGAGAGTGATTGCTCTTCAGAGTCTTAAGTTTGTTGCAAATATTGAAGGTGTCTCTGTTTGGAGAGTGATTGCTCTTCAGAG

TGTGGATTGCTTTGTTGCTTCTTCCACTTTTGTAGAAGAGGAACATGTTAATAGAATGGATGTGGATTGCTTTGTTGCTTCTTCCACTTTTGTAGAAGAGGAACATGTTAATAGAATGGA

TACATTCTGCTTCAATGTACGCAATAGTGTTACTGATGAGTGTCGTCTGGCCATGTTGGGTACATTCTGCTTCAATGTACGCAATAGTGTTACTGATGAGTGTCGTCTGGCCATGTTGGG

TGCTGAAATGACTAGTAATGTCAGAAGACAAGTTGCTTCAGGTGTCATAGACATTAGTACTGCTGAAATGACTAGTAATGTCAGAAGACAAGTTGCTTCAGGTGTCATAGACATTAGTAC

CGGTTGGTTTGATGTTTATGATGACATCTTTGCTGAAAGCAAACCATGGTTTGTTCGCAACGGTTGGTTTGATGTTTATGATGACATCTTTGCTGAAAGCAAACCATGGTTTGTTCGCAA

GGCTGAAGACATTTTTGGCCCTTGTTGGTCCGCTCTTGTTTCTGCACTTAAACAACTTAAGGCTGAAGACATTTTTGGCCCTTGTTGGTCCGCTCTTGTTTCTGCACTTAAACAACTTAA

AGTCACTACAGGTGAACTTGTGAGATTTGTTAAGTCTATTTGCAATTCAGCTGTTGCTGTAGTCACTACAGGTGAACTTGTGAGATTTGTTAAGTCTATTTGCAATTCAGCTGTTGCTGT

CGTGGGTGGTACTATACAAATTCTCGCTAGTGTGCCTGAGAAGTTTTTGAATGCGTTTGACGTGGGTGGTACTATACAAATTCTCGCTAGTGTGCCTGAGAAGTTTTTGAATGCGTTTGA

CGTGTTTGTCACAGCTATTCAAACTGTCTTTGACTGTGCTGTTGAAACTTGTACTATTGCCGTGTTTGTCACAGCTATTCAAACTGTCTTTGACTGTGCTGTTGAAACTTGTACTATTGC

CGGTAAAGCATTTGACAAGGTTTTTGACTATGTCTTGCTTGATAATGCGCTTGTAAAACTCGGTAAAGCATTTGACAAGGTTTTTGACTATGTCTTGCTTGATAATGCGCTTGTAAAACT

TGTCACCACAAAGCTTAAGGGTGTTCGTGAACGTGGCCTTAATAAAGTTAAGTATGCAACTGTCACCACAAAGCTTAAGGGTGTTCGTGAACGTGGCCTTAATAAAGTTAAGTATGCAAC

AGTTGTTGTTGGTTCCACTGAAGAAGTTAAATCTTCACGTGTTGAACGTAGCACTGCTGTAGTTGTTGTTGGTTCCACTGAAGAAGTTAAATCTTCACGTGTTGAACGTAGCACTGCTGT

ACTTACAATCGCCAACAATTATTCCAAACTTTTTGATGAAGGGTATACTGTTGTAATTGGACTTACAATCGCCAACAATTATTCCAAACTTTTTGATGAAGGGTATACTGTTGTAATTGG

CGATGTGGCGTACTTTGTTAGTGACGGCTACTTCCGTCTTATGGCCAGTCCAAATAGTGTCGATGTGGCGTACTTTGTTAGTGACGGCTACTTCCGTCTTATGGCCAGTCCAAATAGTGT

GTTGACTACTGCAGTCTATAAACCATTGTTTGCTTTTAATGTGAATGTTATGGGTACTAGGTTGACTACTGCAGTCTATAAACCATTGTTTGCTTTTAATGTGAATGTTATGGGTACTAG

ACCTGAAAAATTTCCAACCACTGTGACTTGTGAAAATTTAGAGTCTGTTGTTTTGTTTGTACCTGAAAAATTTCCAACCACTGTGACTTGTGAAAATTTAGAGTCTGTTGTTTTGTTTGT

TAATGACAAAATTACTGAATTCCAATTGGATTACAGTATTGATGTCATTGATAATGAAATTAATGACAAAATTACTGAATTCCAATTGGATTACAGTATTGATGTCATTGATAATGAAAT

AATTGTCAAACCTAATATCAGCCTATGTGTTCCACTTTATGTGAGAGACTATGTTGACAAAATTGTCAAACCTAATATCAGCCTATGTGTTCCACTTTATGTGAGAGACTATGTTGACAA

ATGGGATGATTTTTGCAGACAATATAGTAACGAGTCTTGGTTTGAGGATGATTACAGGGCATGGGATGATTTTTGCAGACAATATAGTAACGAGTCTTGGTTTGAGGATGATTACAGGGC

TTTTATCAGTGTTTTGGACATCACTGATGCTGCTGTGAAAGCTGCAGAGTCTAAAGCTTTTTTTATCAGTGTTTTGGACATCACTGATGCTGCTGTGAAAGCTGCAGAGTCTAAAGCTTT

CGTTGATACTATTGTTCCACCTTGCCCATCTATTTTGAAAGTTATAGATGGAGGCAAAATCGTTGATACTATTGTTCCACCTTGCCCATCTATTTTGAAAGTTATAGATGGAGGCAAAAT

ATGGAATGGTGTTATTAAAAATGTTAACTCTGTTAGAGACTGGCTTAAGTCTTTGAAGTTATGGAATGGTGTTATTAAAAATGTTAACTCTGTTAGAGACTGGCTTAAGTCTTTGAAGTT

AAATCTCACACAACAGGGTTTGCTTGGAACATGTGCAAAGCGTTTTAAACGTTGGCTTGGAAATCTCACACAACAGGGTTTGCTTGGAACATGTGCAAAGCGTTTTAAACGTTGGCTTGG

CATTTTGCTAGAGGCCTATAATGCGTTTTTAGACACTGTGGTTTCTACTGTTAAAATTGGCATTTTGCTAGAGGCCTATAATGCGTTTTTAGACACTGTGGTTTCTACTGTTAAAATTGG

TGGCTTGACCTTTAAAACATATGCTTTTGATAAACCTTACATTGTGATACGTGATATCGTTGGCTTGACCTTTAAAACATATGCTTTTGATAAACCTTACATTGTGATACGTGATATCGT

GTGTAAGGTTGAAAATAAAACAGAAGCAGAATGGATTGAGCTTTTTCCACATAATGACAGGTGTAAGGTTGAAAATAAAACAGAAGCAGAATGGATTGAGCTTTTTCCACATAATGACAG

GATTAAGTCTTTTAGTACTTTCGAGAGTGCTTACATGCCAATTGCAGACCCTACACATTTGATTAAGTCTTTTAGTACTTTCGAGAGTGCTTACATGCCAATTGCAGACCCTACACATTT

TGACATTGAAGAAGTTGAACTTTTAGATGCAGAGTTTGTAGAACCAGGCTGTGGTGGTATTGACATTGAAGAAGTTGAACTTTTAGATGCAGAGTTTGTAGAACCAGGCTGTGGTGGTAT

TTTGGCAGTAATAGATGAGCACGTCTTTTATAAGAAGGATGGTGTTTATTATCCATCAAATTTGGCAGTAATAGATGAGCACGTCTTTTATAAGAAGGATGGTGTTTATTATCCATCAAA

TGGTACTAACATTCTACCTGTTGCATTTACAAAAGCCGCTGGTGGTAAAGTTTCATTTTCTGGTACTAACATTCTACCTGTTGCATTTACAAAAGCCGCTGGTGGTAAAGTTTCATTTTC

TGATGACGTTGAAGTAAAAGACATTGAACCTGTTTACAGAGTCAAGCTTTGCTTTGAGTTTGATGACGTTGAAGTAAAAGACATTGAACCTGTTTACAGAGTCAAGCTTTGCTTTGAGTT

TGAAGATGAAAAACTTGTAGATGTTTGTGAAAAGGCAATTGGCAAGAAAATTAAACATGATGAAGATGAAAAACTTGTAGATGTTTGTGAAAAGGCAATTGGCAAGAAAATTAAACATGA

AGGTGACTGGGATAGCTTTTGTAAGACTATTCAATCAGCACTTTCTGTTGTTTCTTGCTAAGGTGACTGGGATAGCTTTTGTAAGACTATTCAATCAGCACTTTCTGTTGTTTCTTGCTA

TGTAAATCTACCTACTTATTACATTTATGATGAAGAAGGCGGTAATGACTTGAGTTTGCCTGTAAATCTACCTACTTATTACATTTATGATGAAGAAGGCGGTAATGACTTGAGTTTGCC

CGTTATGATTTCTGAATGGCCTCTTTCTGTTCAACAAGCTCAACAAGAAGCTACTTTACCCGTTATGATTTCTGAATGGCCTCTTTCTGTTCAACAAGCTCAACAAGAAGCTACTTTACC

TGATATTGCTGAGGATGTTGTTGACCAAGTTGAAGAAGTCAATAGCATTTTTGACATTGATGATATTGCTGAGGATGTTGTTGACCAAGTTGAAGAAGTCAATAGCATTTTTGACATTGA

GACAGTGGATGTTAAACATGATGTGAGTCCTTTTGAAATGCCATTTGAAGAGTTAAATGGGACAGTGGATGTTAAACATGATGTGAGTCCTTTTGAAATGCCATTTGAAGAGTTAAATGG

TTTAAAGATACTCAAACAATTGGATAACAACTGCTGGGTTAACTCAGTTATGTTACAAATTTTAAAGATACTCAAACAATTGGATAACAACTGCTGGGTTAACTCAGTTATGTTACAAAT

ACAATTAACTGGTATACTTGATGGTGACTATGCTATGCAGTTTTTTAAAATGGGCCGAGTACAATTAACTGGTATACTTGATGGTGACTATGCTATGCAGTTTTTTAAAATGGGCCGAGT

TGCCAAGATGATTGAACGCTGCTACACTGCTGAGCAATGTATACGTGGTGCTATGGGTGATGCCAAGATGATTGAACGCTGCTACACTGCTGAGCAATGTATACGTGGTGCTATGGGTGA

TGTTGGTTTGTGTATGTATAGACTGCTTAAAGACTTACACACTGGTTTTATGGTTATGGATGTTGGTTTGTGTATGTATAGACTGCTTAAAGACTTACACACTGGTTTTATGGTTATGGA

TTATAAATGTAGTTGTACCAGTGGTAGGCTTGAAGAATCGGGAGCTGTTTTGTTTTGTACTTATAAATGTAGTTGTACCAGTGGTAGGCTTGAAGAATCGGGAGCTGTTTTGTTTTGTAC

GCCCACTAAGAAGGCGTTTCCTTATGGTACTTGTCTAAATTGTAACGCACCTCGCATGTGGCCCACTAAGAAGGCGTTTCCTTATGGTACTTGTCTAAATTGTAACGCACCTCGCATGTG

TACAATTAGGCAGTTACAAGGTACCATAATATTTGTGCAACAAAAACCAGAACCTGTTAATACAATTAGGCAGTTACAAGGTACCATAATATTTGTGCAACAAAAACCAGAACCTGTTAA

TCCTGTTTCTTTTGTTGTTAAACCAGTCTGCTCATCAATTTTTCGTGGTGCTGTGTCTTGTCCTGTTTCTTTTGTTGTTAAACCAGTCTGCTCATCAATTTTTCGTGGTGCTGTGTCTTG

TGGTCATTACCAGACTAACATCTATTCACAAAATTTGTGTGTGGATGGTTTTGGTGTTAATGGTCATTACCAGACTAACATCTATTCACAAAATTTGTGTGTGGATGGTTTTGGTGTTAA

CAAGATTCAGCCCTGGACAAATGATGCACTTAATACTATTTGTATTAAGGATGCAGATTACAAGATTCAGCCCTGGACAAATGATGCACTTAATACTATTTGTATTAAGGATGCAGATTA

TAATGCAAAAGTTGAAATATCTGTTACACCAATTAAAAATACAGTTGATACAACACCTAATAATGCAAAAGTTGAAATATCTGTTACACCAATTAAAAATACAGTTGATACAACACCTAA

GGAAGAATTTGTTGTTAAAGAGAAGTTGAACGCCTTCCTCGTTCATGACAATGTAGCTTTGGAAGAATTTGTTGTTAAAGAGAAGTTGAACGCCTTCCTCGTTCATGACAATGTAGCTTT

CTACCAAGGTGATGTTGATACTGTTGTTAATGGTGTTGACTTTGACTTTATTGTAAATGCCTACCAAGGTGATGTTGATACTGTTGTTAATGGTGTTGACTTTGACTTTATTGTAAATGC

TGCTAATGAGAACCTTGCTCATGGTGGAGGACTTGCCAAAGCTTTAGATGTGTACACTAATGCTAATGAGAACCTTGCTCATGGTGGAGGACTTGCCAAAGCTTTAGATGTGTACACTAA

AGGTAAACTTCAACGTTTATCTAAAGAACACATTGGATTAGCGGGTAAAGTAAAAGTTGGAGGTAAACTTCAACGTTTATCTAAAGAACACATTGGATTAGCGGGTAAAGTAAAAGTTGG

TACAGGAGTTATGGTTGAGTGTGATAGCCTTAGAATTTTTAATGTTGTTGGTCCACGCAATACAGGAGTTATGGTTGAGTGTGATAGCCTTAGAATTTTTAATGTTGTTGGTCCACGCAA

GGGTAAACATGAACGTGATTTACTCATAAAAGCTTACAACACTATTAATAATGAACAAGGGGGTAAACATGAACGTGATTTACTCATAAAAGCTTACAACACTATTAATAATGAACAAGG

CACACCTTTAACACCAATTTTGAGCTGTGGTATTTTTGGTATCAAACTCGAAACTTCATTCACACCTTTAACACCAATTTTGAGCTGTGGTATTTTTGGTATCAAACTCGAAACTTCATT

AGAAGTTTTGCTTGATGTTTGTAATACAAAAGAAGTTAAAGTTTTTGTTTATACAGACACAGAAGTTTTGCTTGATGTTTGTAATACAAAAGAAGTTAAAGTTTTTGTTTATACAGACAC

AGAGGTTTGTAAGGTTAAGGATTTTGTGTCTGGTTTAGTGAATGTTCAAAAAGTTGAGCAAGAGGTTTGTAAGGTTAAGGATTTTGTGTCTGGTTTAGTGAATGTTCAAAAAGTTGAGCA

ACCTAAAATAGAACCAAAACCAGTGTCCGTAATTAAAGTTGCACCCAAGCCTTACAGGGTACCTAAAATAGAACCAAAACCAGTGTCCGTAATTAAAGTTGCACCCAAGCCTTACAGGGT

AGATGGTAAATTTAGTTACTTTACAGAAGACTTGTTGTGTGTCGCTGATGACAAACCCATAGATGGTAAATTTAGTTACTTTACAGAAGACTTGTTGTGTGTCGCTGATGACAAACCCAT

TGTTTTGTTTACTGACTCTATGCTTACTTTGGATGACCGTGGTTTAGCTCTAGACAATGCTGTTTTGTTTACTGACTCTATGCTTACTTTGGATGACCGTGGTTTAGCTCTAGACAATGC

ACTTAGTGGTGTGCTTAGTGCTGCTATTAAGGATTGTGTTGACATAAATAAAGCTATACCACTTAGTGGTGTGCTTAGTGCTGCTATTAAGGATTGTGTTGACATAAATAAAGCTATACC

TTCTGGTAATCTTATTAAGTTTGATATAGGTTCTGTTGTTGTCTACATGTGTGTTGTGCCTTCTGGTAATCTTATTAAGTTTGATATAGGTTCTGTTGTTGTCTACATGTGTGTTGTGCC

ATCCGAAAAGGACAAACATTTAGATAATAATGTTCAACGATGCACACGTAAGTTGAATAGATCCGAAAAGGACAAACATTTAGATAATAATGTTCAACGATGCACACGTAAGTTGAATAG

ACTTATGTGTGATATAGTTTGTACTATACCAGCTGACTACATCTTGCCATTGGTGTTGTCACTTATGTGTGATATAGTTTGTACTATACCAGCTGACTACATCTTGCCATTGGTGTTGTC

TAGTTTGACTTGTAATGTTTCTTTTGTAGGTGAACTTAAAGCTGCTGAAGCTAAAGTTATTAGTTTGACTTGTAATGTTTCTTTTGTAGGTGAACTTAAAGCTGCTGAAGCTAAAGTTAT

AACTATAAAGGTGACAGAGGATGGTGTTAATGTTCATGATGTGACCGTGACAACAGACAAAACTATAAAGGTGACAGAGGATGGTGTTAATGTTCATGATGTGACCGTGACAACAGACAA

GTCATTTGAACAACAAGTTGGTGTTATTGCTGATAAGGACAAAGATCTTTCTGGTGCAGTGTCATTTGAACAACAAGTTGGTGTTATTGCTGATAAGGACAAAGATCTTTCTGGTGCAGT

ACCAAGTGATCTTAACACATCTGAATTGCTTACTAAAGCAATAGATGTTGATTGGGTCGAACCAAGTGATCTTAACACATCTGAATTGCTTACTAAAGCAATAGATGTTGATTGGGTCGA

ATTTTATGGCTTTAAAGATGCTGTTACTTTTGCAACAGTTGATCATAGTGCTTTTGCCTAATTTTATGGCTTTAAAGATGCTGTTACTTTTGCAACAGTTGATCATAGTGCTTTTGCCTA

TGAAAGTGCTGTTGTTAATGGTATTAGAGTGTTAAAAACTAGTGATAATAATTGTTGGGTTGAAAGTGCTGTTGTTAATGGTATTAGAGTGTTAAAAACTAGTGATAATAATTGTTGGGT

GAATGCTGTTTGTATTGCACTACAGTATTCGAAACCCCATTTTATTTCACAAGGTCTTGAGAATGCTGTTTGTATTGCACTACAGTATTCGAAACCCCATTTTATTTCACAAGGTCTTGA

TGCTGCGTGGAATAAATTTGTTTTAGGCGATGTTGAAATTTTTGTTGCATTTGTTTACTATGCTGCGTGGAATAAATTTGTTTTAGGCGATGTTGAAATTTTTGTTGCATTTGTTTACTA

TGTTGCAAGACTAATGAAAGGTGACAAGGGTGATGCTGAAGACACTTTGACTAAGTTGTCTGTTGCAAGACTAATGAAAGGTGACAAGGGTGATGCTGAAGACACTTTGACTAAGTTGTC

TAAGTATCTTGCTAATGAAGCTCAAGTTCAATTAGAACATTATAGTTCTTGTGTTGAATGTAAGTATCTTGCTAATGAAGCTCAAGTTCAATTAGAACATTATAGTTCTTGTGTTGAATG

TGATGCTAAATTTAAAAACTCTGTTGCATCTATCAATTCTGCTATAGTTTGTGCTAGTGTTGATGCTAAATTTAAAAACTCTGTTGCATCTATCAATTCTGCTATAGTTTGTGCTAGTGT

CAAACGTGATGGTGTGCAAGTTGGTTATTGTGTCCATGGTATTAAGTACTATTCACGTGTCAAACGTGATGGTGTGCAAGTTGGTTATTGTGTCCATGGTATTAAGTACTATTCACGTGT

TAGAAGTGTTAGAGGTAGAGCTATTATAGTCAGTGTCGAACAGCTTGAACCGTGTGCTCATAGAAGTGTTAGAGGTAGAGCTATTATAGTCAGTGTCGAACAGCTTGAACCGTGTGCTCA

GTCTAGACTTTTGAGTGGTGTTGCTTATACTGCTTTTTCTGGACCTGTTGACAAAGGTCAGTCTAGACTTTTGAGTGGTGTTGCTTATACTGCTTTTTCTGGACCTGTTGACAAAGGTCA

TTATACTGTTTATGATACTGCAAAGAAATCAATGTATGATGGTGATCGTTTTGTTAAACATTATACTGTTTATGATACTGCAAAGAAATCAATGTATGATGGTGATCGTTTTGTTAAACA

TGATCTTTCTCTGCTGTCTGTCACATCAGTTGTTATGGTTGGTGGTTATGTTGCACCTGTTGATCTTTCTCTGCTGTCTGTCACATCAGTTGTTATGGTTGGTGGTTATGTTGCACCTGT

TAATACAGTGAAACCTAAACCAGTCATTAATCAACTTGATGAAAAGGCACAGAAGTTCTTTAATACAGTGAAACCTAAACCAGTCATTAATCAACTTGATGAAAAGGCACAGAAGTTCTT

TGATTTTGGTGATTTTTTGATTCATAATTTTGTTATTTTTTTCACATGGTTATTGAGTATTGATTTTGGTGATTTTTTGATTCATAATTTTGTTATTTTTTTCACATGGTTATTGAGTAT

GTTTACTTTGTGTAAAACTGCAGTAACTACAGGTGATGTTAAAATAATGGCCAAAGCACCGTTTACTTTGTGTAAAACTGCAGTAACTACAGGTGATGTTAAAATAATGGCCAAAGCACC

ACAAAGGACGGGTGTTGTTTTAAAACGTAGTCTTAAATATAACTTAAAAGCGTCAGCAGCACAAAGGACGGGTGTTGTTTTAAAACGTAGTCTTAAATATAACTTAAAAGCGTCAGCAGC

TGTTCTTAAATCTAAGTGGTGGCTGCTTGCTAAGTTTACGAAACTACTGTTACTCATATATGTTCTTAAATCTAAGTGGTGGCTGCTTGCTAAGTTTACGAAACTACTGTTACTCATATA

TACATTGTACTCAGTAGTTTTGCTTTGTGTACGTTTTGGACCGTTTAATTTTTGTAGTGATACATTGTACTCAGTAGTTTTGCTTTGTGTACGTTTTGGACCGTTTAATTTTTGTAGTGA

GACTGTTAATGGTTATGCTAAGTCAAACTTTGTCAAGGATGATTACTGTGATGGTTCATTGACTGTTAATGGTTATGCTAAGTCAAACTTTGTCAAGGATGATTACTGTGATGGTTCATT

GGGCTGCAAGATGTGTCTTTTTGGTTACCAAGAGTTAAGTCAATTTAGCCATTTGGATGTGGGCTGCAAGATGTGTCTTTTTGGTTACCAAGAGTTAAGTCAATTTAGCCATTTGGATGT

TGTGTGGAAGCATATAACAGACCCTTTGTTTAGTAATATGCAACCTTTCATTGTCATGGTTGTGTGGAAGCATATAACAGACCCTTTGTTTAGTAATATGCAACCTTTCATTGTCATGGT

TTTGCTGCTTATATTTGGTGACAATTATTTGAGATGCTTCTTGCTGTATTTTGTTGCTCATTTGCTGCTTATATTTGGTGACAATTATTTGAGATGCTTCTTGCTGTATTTTGTTGCTCA

GATGATAAGCACAGTTGGTGTTTTTCTAGGTTACAAGGAAACAAATTGGTTCTTGCACTTGATGATAAGCACAGTTGGTGTTTTTCTAGGTTACAAGGAAACAAATTGGTTCTTGCACTT

TATTCCATTTGATGTTATTTGTGATGAACTGCTTGTCACTGTTATTGTTATTAAGGTTATTATTCCATTTGATGTTATTTGTGATGAACTGCTTGTCACTGTTATTGTTATTAAGGTTAT

TTCTTTTGTCAGACATGTGCTTTTTGGTTGTGAAAACCCAGATTGCATTGCGTGTTCTAATTCTTTTGTCAGACATGTGCTTTTTGGTTGTGAAAACCCAGATTGCATTGCGTGTTCTAA

GAGTGCTAGACTTAAGAGATTCCCTGTTAACACAATTGTCAATGGTGTGCAACGTTCATTGAGTGCTAGACTTAAGAGATTCCCTGTTAACACAATTGTCAATGGTGTGCAACGTTCATT

TTATGTTAATGCAAATGGTGGTAGTAAGTTTTGTAAGAAACATAGATTTTTCTGTGTTGATTATGTTAATGCAAATGGTGGTAGTAAGTTTTGTAAGAAACATAGATTTTTCTGTGTTGA

TTGTGACTCTTATGGTTATGGCAGCACGTTTATAACACCCGAAGTTTCTAGAGAACTTGGTTGTGACTCTTATGGTTATGGCAGCACGTTTATAACACCCGAAGTTTCTAGAGAACTTGG

TAACATTACCAAAACAAATGTGCAACCAACAGGCCCAGCGTATGTCATGATTGACAAAGTTAACATTACCAAAACAAATGTGCAACCAACAGGCCCAGCGTATGTCATGATTGACAAAGT

GGAGTTTGAAAATGGTTTTTACAGATTGTATTCCTGTGAAACATTTTGGCGTTACAACTTGGAGTTTGAAAATGGTTTTTACAGATTGTATTCCTGTGAAACATTTTGGCGTTACAACTT

TGATATAACTGAAAGCAAGTATTCTTGCAAAGAGGTTTTTAAAAATTGTAATGTTTTGGATGATATAACTGAAAGCAAGTATTCTTGCAAAGAGGTTTTTAAAAATTGTAATGTTTTGGA

TGATTTCATCGTGTTTAACAATAATGGGACCAATGTAACGCAGGTTAAAAATGCTAGTGTTGATTTCATCGTGTTTAACAATAATGGGACCAATGTAACGCAGGTTAAAAATGCTAGTGT

TTACTTTTCACAGTTGTTGTGTAGGCCCATTAAATTAGTTGACAGTGAACTTTTGTCCACTTACTTTTCACAGTTGTTGTGTAGGCCCATTAAATTAGTTGACAGTGAACTTTTGTCCAC

TTTGTCAGTTGATTTTAATGGTGTCTTACACAAGGCATACATTGATGTACTACGTAATAGTTTGTCAGTTGATTTTAATGGTGTCTTACACAAGGCATACATTGATGTACTACGTAATAG

CTTTGGTAAAGATCTTAATGCTAATATGTCTTTAGCCGAGTGCAAGAGTGCTTTAGGCCTCTTTGGTAAAGATCTTAATGCTAATATGTCTTTAGCCGAGTGCAAGAGTGCTTTAGGCCT

GTCTATTAGTGATCATGAATTTACTAGTGCTATTTCTAATGCACATCGTTGTGACGTGTTGTCTATTAGTGATCATGAATTTACTAGTGCTATTTCTAATGCACATCGTTGTGACGTGTT

GTTATCTGATTTGTCATTTAACAACTTTGTCAGTTCGTATGCTAAACCTGAGGAAAAATTGTTATCTGATTTGTCATTTAACAACTTTGTCAGTTCGTATGCTAAACCTGAGGAAAAATT

ATCAGCTTATGACTTGGCGTGTTGTATGCGTGCAGGTGCTAAGGTTGTTAATGCCAATGTATCAGCTTATGACTTGGCGTGTTGTATGCGTGCAGGTGCTAAGGTTGTTAATGCCAATGT

TCTGACAAAGGACCAAACTCCTATTGTTTGGCATGCAAAGGATTTTAACAGTCTTTCTGCTCTGACAAAGGACCAAACTCCTATTGTTTGGCATGCAAAGGATTTTAACAGTCTTTCTGC

TGAAGGTCGCAAGTATATTGTAAAAACTAGCAAAGCTAAGGGTTTGACTTTCTTGTTGACTGAAGGTCGCAAGTATATTGTAAAAACTAGCAAAGCTAAGGGTTTGACTTTCTTGTTGAC

AATTAATGAAAACCAAGCTGTCACGCAAATACCTGCAACTAGCATTGTTGCTAAGCAAGGAATTAATGAAAACCAAGCTGTCACGCAAATACCTGCAACTAGCATTGTTGCTAAGCAAGG

TGCTGGTGATGCTGGCCATTCATTAACATGGCTGTGGCTACTGTGTGGTCTTGTGTGTTTTGCTGGTGATGCTGGCCATTCATTAACATGGCTGTGGCTACTGTGTGGTCTTGTGTGTTT

GATTCAATTCTACTTGTGCTTTTTCATGCCCTATTTTATGTACGATATCGTGAGTAGTTTGATTCAATTCTACTTGTGCTTTTTCATGCCCTATTTTATGTACGATATCGTGAGTAGTTT

TGAGGGTTATGATTTTAAGTATATAGAAAATGGTCAGTTGAAGAATTTTGAAGCGCCACTTGAGGGTTATGATTTTAAGTATATAGAAAATGGTCAGTTGAAGAATTTTGAAGCGCCACT

TAAATGCGTCAGAAACGTTTTTGAAAACTTTGAGGACTGGCATTATGCTAAGTTTGGCTTTAAATGCGTCAGAAACGTTTTTGAAAACTTTGAGGACTGGCATTATGCTAAGTTTGGCTT

CACACCTTTAAACAAGCAAAGCTGTCCTATTGTAGTTGGAGTTTCTGAAATTGTTAATACCACACCTTTAAACAAGCAAAGCTGTCCTATTGTAGTTGGAGTTTCTGAAATTGTTAATAC

TGTCGCTGGCATTCCATCTAATGTGTATCTTGTTGGTAAAACTTTAATTTTTACACTACATGTCGCTGGCATTCCATCTAATGTGTATCTTGTTGGTAAAACTTTAATTTTTACACTACA

AGCTGCTTTTGGTAATGCTGGTGTTTGTTATGACATTTTTGGAGTCACAACACCTGAAAAAGCTGCTTTTGGTAATGCTGGTGTTTGTTATGACATTTTTGGAGTCACAACACCTGAAAA

GTGCATTTTTACTTCTGCTTGTACTAGATTAGAAGGTTTGGGTGGTGACAATGTTTATTGGTGCATTTTTACTTCTGCTTGTACTAGATTAGAAGGTTTGGGTGGTGACAATGTTTATTG

TTATAACACAGCGCTTATGGAAGGTTCTTTGCCTTACAGTTCAATACAAGCTAATGCATATTATAACACAGCGCTTATGGAAGGTTCTTTGCCTTACAGTTCAATACAAGCTAATGCATA

TTATAAATATGACAATGGCAATTTTATTAAGTTGCCAGAAGTTATTGCACAAGGCTTTGGTTATAAATATGACAATGGCAATTTTATTAAGTTGCCAGAAGTTATTGCACAAGGCTTTGG

TTTTAGAACAGTGCGTACTATTGCCACCAAATACTGCCGCGTAGGTGAATGTGTTGAATCTTTTAGAACAGTGCGTACTATTGCCACCAAATACTGCCGCGTAGGTGAATGTGTTGAATC

CAATGCAGGTGTGTGTTTTGGCTTTGACAAGTGGTTTGTTAACGATGGACGTGTTGCCAACAATGCAGGTGTGTGTTTTGGCTTTGACAAGTGGTTTGTTAACGATGGACGTGTTGCCAA

TGGTTACGTTTGTGGTACTGGTTTGTGGAACCTTGTATTTAACATACTTTCCATGTTTTCTGGTTACGTTTGTGGTACTGGTTTGTGGAACCTTGTATTTAACATACTTTCCATGTTTTC

ATCTTCATTCTCTGTTGCTGCAATGTCAGGTCAAATTTTACTTAATTGTGCATTAGGTGCATCTTCATTCTCTGTTGCTGCAATGTCAGGTCAAATTTTACTTAATTGTGCATTAGGTGC

TTTTGCTATTTTTTGTTGTTTTCTTGTGACAAAGTTTAGACGCATGTTTGGTGACCTTTCTTTTGCTATTTTTTGTTGTTTTCTTGTGACAAAGTTTAGACGCATGTTTGGTGACCTTTC

TGTAGGTGTTTGCACTGTTGTTGTGGCTGTTTTGCTTAACAATGTCTCTTACATTGTAACTGTAGGTGTTTGCACTGTTGTTGTGGCTGTTTTGCTTAACAATGTCTCTTACATTGTAAC

TCAGAATTTAGTAACAATGATTGCTTATGCCATATTGTATTTCTTTGCTACTAGAAGCTTTCAGAATTTAGTAACAATGATTGCTTATGCCATATTGTATTTCTTTGCTACTAGAAGCTT

ACGCTATGCATGGATTTGGTGTGCTGCATATTTAATTGCGTATATTTCTTTTGCTCCATGACGCTATGCATGGATTTGGTGTGCTGCATATTTAATTGCGTATATTTCTTTTGCTCCATG

GTGGTTGTGTGCTTGGTACTTTCTTGCTATGTTGACAGGTTTGTTACCTAGTTTGCTGAAGTGGTTGTGTGCTTGGTACTTTCTTGCTATGTTGACAGGTTTGTTACCTAGTTTGCTGAA

GCTTAAAGTTTCGACAAATCTTTTCGAAGGTGACAAATTTGTAGGTACATTTGAAAGTGCGCTTAAAGTTTCGACAAATCTTTTCGAAGGTGACAAATTTGTAGGTACATTTGAAAGTGC

TGCTGCAGGAACATTTGTCATTGACATGCGTTCTTATGAGAAACTTGCTAATAGCATCTCTGCTGCAGGAACATTTGTCATTGACATGCGTTCTTATGAGAAACTTGCTAATAGCATCTC

TCCAGAAAAGTTGAAAAGTTATGCTGCTAGCTATAATAGATATAAGTACTATAGTGGTAATCCAGAAAAGTTGAAAAGTTATGCTGCTAGCTATAATAGATATAAGTACTATAGTGGTAA

TGCAAATGAAGCTGATTACTGTTGCGCTTGTTATGCCTATTTAGCAAAAGCAATGTTGGATGCAAATGAAGCTGATTACTGTTGCGCTTGTTATGCCTATTTAGCAAAAGCAATGTTGGA

CTTTTCGCGTGATCATAATGACATCTTGTACACACCTCCGACTGTCAGTTATGGTTCTACCTTTTCGCGTGATCATAATGACATCTTGTACACACCTCCGACTGTCAGTTATGGTTCTAC

ATTACAGGCTGGTTTGCGCAAAATGGCACAACCATCTGGCTTTGTGGAGAAATGTGTTGTATTACAGGCTGGTTTGCGCAAAATGGCACAACCATCTGGCTTTGTGGAGAAATGTGTTGT

CCGTGTCTGCTATGGAAACACTGTGTTGAATGGGTTGTGGCTTGGTGATATTGTTTATTGCCGTGTCTGCTATGGAAACACTGTGTTGAATGGGTTGTGGCTTGGTGATATTGTTTATTG

CCCACGTCATGTTATCGCATCTAACACAACTTCTGCTATAGATTATGATCACGAATATAGCCCACGTCATGTTATCGCATCTAACACAACTTCTGCTATAGATTATGATCACGAATATAG

TATTATGCGGTTGCATAATTTTTCTATAATATCTGGTACAGCATTTCTTGGTGTTGTAGGTATTATGCGGTTGCATAATTTTTCTATAATATCTGGTACAGCATTTCTTGGTGTTGTAGG

TGCTACTATGCATGGAGTAACTCTTAAAATTAAGGTTTCACAGACTAACATGCACACACCTGCTACTATGCATGGAGTAACTCTTAAAATTAAGGTTTCACAGACTAACATGCACACACC

TAGACATTCTTTTAGAACACTAAAATCTGGTGAAGGTTTTAACATCTTAGCATGCTATGATAGACATTCTTTTAGAACACTAAAATCTGGTGAAGGTTTTAACATCTTAGCATGCTATGA

TGGTTGTGCTCAAGGTGTTTTTGGTGTGAACATGAGAACTAATTGGACTATCCGTGGTTCTGGTTGTGCTCAAGGTGTTTTTGGTGTGAACATGAGAACTAATTGGACTATCCGTGGTTC

ATTTATTAATGGTGCGTGTGGTTCCCCTGGCTACAATCTTAAAAATGGCGAGGTGGAATTATTTATTAATGGTGCGTGTGGTTCCCCTGGCTACAATCTTAAAAATGGCGAGGTGGAATT

TGTTTATATGCATCAAATTGAACTCGGAAGTGGTAGCCATGTAGGTTCTAGCTTTGATGGTGTTTATATGCATCAAATTGAACTCGGAAGTGGTAGCCATGTAGGTTCTAGCTTTGATGG

TGTTATGTATGGTGGTTTTGAAGACCAACCTAATCTTCAAGTTGAATCTGCAAACCAGATTGTTATGTATGGTGGTTTTGAAGACCAACCTAATCTTCAAGTTGAATCTGCAAACCAGAT

GTTAACAGTTAATGTGGTTGCATTTCTTTATGCTGCTATATTGAATGGTTGCACATGGTGGTTAACAGTTAATGTGGTTGCATTTCTTTATGCTGCTATATTGAATGGTTGCACATGGTG

GCTTAAAGGTGAAAAATTGTTTGTGGAGCATTATAATGAGTGGGCACAGGCTAATGGTTTGCTTAAAGGTGAAAAATTGTTTGTGGAGCATTATAATGAGTGGGCACAGGCTAATGGTTT

CACAGCTATGAATGGTGAAGACGCTTTTTCCATTCTTGCTGCTAAAACTGGTGTCTGTGTCACAGCTATGAATGGTGAAGACGCTTTTTCCATTCTTGCTGCTAAAACTGGTGTCTGTGT

GGAAAGATTACTTCATGCTATTCAAGTTTTGAATAATGGCTTTGGTGGTAAACAAATTTTGGAAAGATTACTTCATGCTATTCAAGTTTTGAATAATGGCTTTGGTGGTAAACAAATTTT

GGGTTATTCTAGTCTCAATGATGAGTTCAGTATTAATGAAGTTGTCAAACAAATGTTTGGGGGTTATTCTAGTCTCAATGATGAGTTCAGTATTAATGAAGTTGTCAAACAAATGTTTGG

TGTTAACCTGCAAAGTGGTAAAACCACTAGTATGTTTAAATCCATAAGCTTATTTGCTGGTGTTAACCTGCAAAGTGGTAAAACCACTAGTATGTTTAAATCCATAAGCTTATTTGCTGG

CTTCTTTGTCATGTTCTGGGCTGAATTATTTGTTTATACCACCACTATTTGGGTTAACCCCTTCTTTGTCATGTTCTGGGCTGAATTATTTGTTTATACCACCACTATTTGGGTTAACCC

TGGTTTTCTTACTCCGTTTATGATTTTGCTTGTTGCTTTGTCACTCTGTCTTACATTTGTTGGTTTTCTTACTCCGTTTATGATTTTGCTTGTTGCTTTGTCACTCTGTCTTACATTTGT

TGTTAAACATAAGGTTTTGTTTTTGCAAGTGTTTTTGTTGCCTTCAATTATTGTGGCTGCTGTTAAACATAAGGTTTTGTTTTTGCAAGTGTTTTTGTTGCCTTCAATTATTGTGGCTGC

TATTCAAAACTGTGCTTGGGACTACCATGTTACAAAGGTGTTGGCAGAGAAGTTTGATTATATTCAAAACTGTGCTTGGGACTACCATGTTACAAAGGTGTTGGCAGAGAAGTTTGATTA

TAATGTTTCTGTTATGCAAATGGACATCCAGGGTTTTGTTAACATTTTTATTTGTCTTTTTAATGTTTCTGTTATGCAAATGGACATCCAGGGTTTTGTTAACATTTTTATTTGTCTTTT

TGTTGCACTGTTGCATACTTGGCGCTTTGCTAAAGAGCGTTGTACACATTGGTGCACTTATGTTGCACTGTTGCATACTTGGCGCTTTGCTAAAGAGCGTTGTACACATTGGTGCACTTA

TTTGTTCTCACTCATTGCTGTTTTATACACTGCATTGTATAGTTATGACTACGTTAGTTTTTTGTTCTCACTCATTGCTGTTTTATACACTGCATTGTATAGTTATGACTACGTTAGTTT

GCTGGTTATGCTACTTTGTGCAATTTCTAATGAATGGTATATTGGTGCTATTATTTTTAGGCTGGTTATGCTACTTTGTGCAATTTCTAATGAATGGTATATTGGTGCTATTATTTTTAG

AATTTGTCGTTTTGGTGTTGCATTTTTACCAGTGGAATACGTGTCTTACTTTGATGGTGTAATTTGTCGTTTTGGTGTTGCATTTTTACCAGTGGAATACGTGTCTTACTTTGATGGTGT

TAAAACTGTGCTGTTGTTTTACATGTTGTTAGGCTTTGTTAGCTGTATGTACTATGGTTTTAAAACTGTGCTGTTGTTTTACATGTTGTTAGGCTTTGTTAGCTGTATGTACTATGGTTT

GTTGTACTGGATTAACAGGTTCTGTAAGTGCACATTAGGTGTTTATGATTTCTGTGTTAGGTTGTACTGGATTAACAGGTTCTGTAAGTGCACATTAGGTGTTTATGATTTCTGTGTTAG

TCCAGCCGAATTTAAGTATATGGTTGCTAATGGTTTGAATGCACCAAATGGCCCTTTTGATCCAGCCGAATTTAAGTATATGGTTGCTAATGGTTTGAATGCACCAAATGGCCCTTTTGA

TGCGCTCTTTCTGTCTTTTAAACTAATGGGTATTGGCGGTCCTAGAACCATTAAAGTTTCTGCGCTCTTTCTGTCTTTTAAACTAATGGGTATTGGCGGTCCTAGAACCATTAAAGTTTC

TACTGTACAGTCTAAATTGACTGATCTTAAGTGCACAAACGTCGTTCTAATGGGCATTTTTACTGTACAGTCTAAATTGACTGATCTTAAGTGCACAAACGTCGTTCTAATGGGCATTTT

GTCTAACATGAACATAGCTTCTAATTCAAAGGAGTGGGCATATTGTGTTGAAATGCACAAGTCTAACATGAACATAGCTTCTAATTCAAAGGAGTGGGCATATTGTGTTGAAATGCACAA

TAAAATAAACTTGTGTGACGACCCTGAAACTGCTCAAGAGTTATTGCTGGCGTTGTTGGCTAAAATAAACTTGTGTGACGACCCTGAAACTGCTCAAGAGTTATTGCTGGCGTTGTTGGC

CTTTTTCTTGTCTAAGCATAGTGATTTTGGTCTTGGTGATCTTGTCGATTCTTATTTTGACTTTTTCTTGTCTAAGCATAGTGATTTTGGTCTTGGTGATCTTGTCGATTCTTATTTTGA

GAACGACTCCATTTTGCAAAGTGTTGCATCTTCTTTTGTTGGTATGCCATCTTTTGTTGCGAACGACTCCATTTTGCAAAGTGTTGCATCTTCTTTTGTTGGTATGCCATCTTTTGTTGC

ATATGAAACAGCAAGACAAGAGTATGAAAATGCTGTTGCAAATGGTTCCTCACCACAAATATATGAAACAGCAAGACAAGAGTATGAAAATGCTGTTGCAAATGGTTCCTCACCACAAAT

AATCAAACAATTGAAGAAGGCTATGAATGTTGCAAAAGCTGAGTTTGACAGGGAATCATCAATCAAACAATTGAAGAAGGCTATGAATGTTGCAAAAGCTGAGTTTGACAGGGAATCATC

TGTTCAAAAGAAAATTAACAGAATGGCTGAACAAGCTGCTGCAGCTATGTACAAAGAAGCTGTTCAAAAGAAAATTAACAGAATGGCTGAACAAGCTGCTGCAGCTATGTACAAAGAAGC

ACGTGCTGTTAATAGAAAATCAAAAGTTGTTAGTGCCATGCATAGTTTACTCTTTGGCATACGTGCTGTTAATAGAAAATCAAAAGTTGTTAGTGCCATGCATAGTTTACTCTTTGGCAT

GCTCCGACGTTTGGACATGTCTAGTGTTGACACTATCCTTAATATGGCACGTAATGGTGTGCTCCGACGTTTGGACATGTCTAGTGTTGACACTATCCTTAATATGGCACGTAATGGTGT

TGTCCCTCTTTCCGTTATCCCTGCTACTTCTGCAGCCAGGCTCGTCGTCGTAGTACCAGATGTCCCTCTTTCCGTTATCCCTGCTACTTCTGCAGCCAGGCTCGTCGTCGTAGTACCAGA

TCATGATTCATTTGTGAAAATGATGGTAGATGGTTTTGTGCACTACGCTGGTGTTGTTTGTCATGATTCATTTGTGAAAATGATGGTAGATGGTTTTGTGCACTACGCTGGTGTTGTTTG

GACATTACAGGAAGTTAAGGATAATGATGGTAAGAATGTGCATCTTAAAGATGTTACAAAGACATTACAGGAAGTTAAGGATAATGATGGTAAGAATGTGCATCTTAAAGATGTTACAAA

GGAAAACCAGGAAATACTTGTTTGGCCTCTGATTTTGACTTGTGAACGTGTCGTTAAATTGGAAAACCAGGAAATACTTGTTTGGCCTCTGATTTTGACTTGTGAACGTGTCGTTAAATT

GCAGAACAATGAAATAATGCCGGGCAAGATGAAGGTCAAGGCCACCAAAGGTGAAGGTGAGCAGAACAATGAAATAATGCCGGGCAAGATGAAGGTCAAGGCCACCAAAGGTGAAGGTGA

TGGAGGCATTACTAGTGAAGGTAATGCTCTATACAACAATGAAGGTGGACGTGCATTCATTGGAGGCATTACTAGTGAAGGTAATGCTCTATACAACAATGAAGGTGGACGTGCATTCAT

GTATGCATATGTGACTACGAAGCCTGGCATGAAGTATGTTAAATGGGAACATGACTCTGGGTATGCATATGTGACTACGAAGCCTGGCATGAAGTATGTTAAATGGGAACATGACTCTGG

TGTGGTTACAGTTGAATTGGAACCACCTTGCAGATTTGTTATAGACACACCTACTGGACCTGTGGTTACAGTTGAATTGGAACCACCTTGCAGATTTGTTATAGACACACCTACTGGACC

CCAAATTAAGTATCTTTATTTTGTTAAGAATCTTAACAATTTAAGGAGAGGTGCTGTTTTCCAAATTAAGTATCTTTATTTTGTTAAGAATCTTAACAATTTAAGGAGAGGTGCTGTTTT

GGGTTACATTGGTGCCACTGTGAGATTGCAAGCTGGCAAACAGACTGAGTTTGTTTCAAAGGGTTACATTGGTGCCACTGTGAGATTGCAAGCTGGCAAACAGACTGAGTTTGTTTCAAA

CTCCCATTTATTAACACATTGTTCTTTTGCTGTTGACCCAGCTGCAGCCTATCTTGATGCCTCCCATTTATTAACACATTGTTCTTTTGCTGTTGACCCAGCTGCAGCCTATCTTGATGC

TGTTAAACAAGGCGCAAAACCTGTTGGCAATTGTGTAAAGATGTTGACTAATGGTTCTGGTGTTAAACAAGGCGCAAAACCTGTTGGCAATTGTGTAAAGATGTTGACTAATGGTTCTGG

TAGCGGTCAGGCTATTACTTGTACCATTGATTCCAACACTACGCAGGACACATATGGTGGTAGCGGTCAGGCTATTACTTGTACCATTGATTCCAACACTACGCAGGACACATATGGTGG

CGCGTCTGTTTGTATTTATTGCAGAGCACATGTTGCACATCCAACCATGGACGGTTTTTGCGCGTCTGTTTGTATTTATTGCAGAGCACATGTTGCACATCCAACCATGGACGGTTTTTG

TCAGTACAAAGGCAAGTGGGTACAAGTGCCTATAGGTACAAATGACCCTATAAGATTTTGTCAGTACAAAGGCAAGTGGGTACAAGTGCCTATAGGTACAAATGACCCTATAAGATTTTG

TCTTGAAAATACTGTTTGTAAAGTTTGTGGTTGTTGGCTTAATCATGGCTGTACATGTGATCTTGAAAATACTGTTTGTAAAGTTTGTGGTTGTTGGCTTAATCATGGCTGTACATGTGA

CCGGACTGCTATCCAAAGTTTTGATAACAGTTATTTAAACGAGTCCGGGGCTCTAGTGCCCCGGACTGCTATCCAAAGTTTTGATAACAGTTATTTAAACGAGTCCGGGGCTCTAGTGCC

GCTCGACTAGAGCCCTGTAATGGTACAGACATAGATTACTGTGTCCGTGCATTTGACGTTGCTCGACTAGAGCCCTGTAATGGTACAGACATAGATTACTGTGTCCGTGCATTTGACGTT

TACAATAAAGATGCGTCTTTTATCGGGAAAAATCTGAAGTCCAATTGTGTGCGCTTCAAGTACAATAAAGATGCGTCTTTTATCGGGAAAAATCTGAAGTCCAATTGTGTGCGCTTCAAG

AATGTAGATAAGGATGACGCGTTCTATATTGTTAAACGTTGCATTAAGTCAGTTATGGACAATGTAGATAAGGATGACGCGTTCTATATTGTTAAACGTTGCATTAAGTCAGTTATGGAC

CACGAGCAGTCCATGTATAACTTACTTAAAGGCTGTAATGCTGTTGCTAAGCATGATTTCCACGAGCAGTCCATGTATAACTTACTTAAAGGCTGTAATGCTGTTGCTAAGCATGATTTC

TTTACTTGGCATGAGGGCAGAACCATTTATGGTAATGTTAGTAGACAGGATCTTACTAAATTTACTTGGCATGAGGGCAGAACCATTTATGGTAATGTTAGTAGACAGGATCTTACTAAA

TACACCATGATGGATTTGTGCTTCGCTCTGCGTAACTTTGATGAAAAAGACTGTGAAGTTTACACCATGATGGATTTGTGCTTCGCTCTGCGTAACTTTGATGAAAAAGACTGTGAAGTT

TTTAAGGAGATATTGGTTCTTACTGGTTGTTGTAGTACTGATTACTTTGAAATGAAGAATTTTAAGGAGATATTGGTTCTTACTGGTTGTTGTAGTACTGATTACTTTGAAATGAAGAAT

TGGTTTGACCCCATAGAAAATGAGGACATACACCGTGTGTATGCTGCTTTAGGTAAGGTATGGTTTGACCCCATAGAAAATGAGGACATACACCGTGTGTATGCTGCTTTAGGTAAGGTA

GTTGCAAATGCAATGCTTAAGTGTGTTGCTTTTTGCGACGAAATGGTGCTCAAAGGAGTTGTTGCAAATGCAATGCTTAAGTGTGTTGCTTTTTGCGACGAAATGGTGCTCAAAGGAGTT

GTTGGTGTTTTGACCTTAGACAACCAAGATCTTAATGGGAATTTCTATGACTTCGGTGACGTTGGTGTTTTGACCTTAGACAACCAAGATCTTAATGGGAATTTCTATGACTTCGGTGAC

TTTGTATTGTGTCCTCCTGGAATGGGAATACCCTACTGCACGTCATACTATTCTTATATGTTTGTATTGTGTCCTCCTGGAATGGGAATACCCTACTGCACGTCATACTATTCTTATATG

ATGCCTGTTATGGGTATGACTAATTGTTTAGCTAGTGAGTGCTTTATGAAAAGTGACATCATGCCTGTTATGGGTATGACTAATTGTTTAGCTAGTGAGTGCTTTATGAAAAGTGACATC

TTTGGTCAAGACTTCAAAACTTTTGATTTGTTGAAATATGATTTCACAGAACATAAGGAGTTTGGTCAAGACTTCAAAACTTTTGATTTGTTGAAATATGATTTCACAGAACATAAGGAG

GTTTTGTTTAACAAGTACTTTAAGTATTGGGGACAGGATTATCATCCTGATTGTGTTGATGTTTTGTTTAACAAGTACTTTAAGTATTGGGGACAGGATTATCATCCTGATTGTGTTGAT

TGCCATGACGAGATGTGTATTTTGCATTGTTCAAATTTTAACACACTCTTCGCAACCACATGCCATGACGAGATGTGTATTTTGCATTGTTCAAATTTTAACACACTCTTCGCAACCACA

ATTCCAAACACGGCTTTTGGACCTCTATGCAGAAAAGTGTTTATTGATGGTGTACCCGTAATTCCAAACACGGCTTTTGGACCTCTATGCAGAAAAGTGTTTATTGATGGTGTACCCGTA

GTTGCTACTGCTGGTTACCACTTTAAACAATTAGGACTTGTGTGGAACAAAGATGTTAACGTTGCTACTGCTGGTTACCACTTTAAACAATTAGGACTTGTGTGGAACAAAGATGTTAAC

ACTCATTCTACCAGACTTACTATTACTGAACTCTTACAGTTTGTGACAGATCCAACGCTTACTCATTCTACCAGACTTACTATTACTGAACTCTTACAGTTTGTGACAGATCCAACGCTT

ATAGTTGCGTCATCGCCTGCCTTGGTGGATAAACGCACTGTTTGTTTTTCTGTCGCTGCTATAGTTGCGTCATCGCCTGCCTTGGTGGATAAACGCACTGTTTGTTTTTCTGTCGCTGCT

TTGAGTACAGGATTAACATCCCAAACAGTAAAACCTGGCCATTTTAATAAGGAGTTTTATTTGAGTACAGGATTAACATCCCAAACAGTAAAACCTGGCCATTTTAATAAGGAGTTTTAT

GACTTCTTACGTTCTCAGGGGTTTTTCGATGAGGGTTCAGAATTAACATTGAAGCATTTCGACTTCTTACGTTCTCAGGGGTTTTTCGATGAGGGTTCAGAATTAACATTGAAGCATTTC

TTTTTTACACAAAAGGGTGATGCTGCAATTAAAGATTTTGATTATTATCGTTACAACAGATTTTTTACACAAAAGGGTGATGCTGCAATTAAAGATTTTGATTATTATCGTTACAACAGA

CCTACTATGCTGGATATTGGACAAGCTCGCGTAGCATATCAAGTGGCAGCTCGCTATTTTCCTACTATGCTGGATATTGGACAAGCTCGCGTAGCATATCAAGTGGCAGCTCGCTATTTT

GACTGTTACGAGGGTGGCTGTATTACATCTAGAGAGGTTGTTGTTACAAACCTTAATAAAGACTGTTACGAGGGTGGCTGTATTACATCTAGAGAGGTTGTTGTTACAAACCTTAATAAA

AGCGCTGGTTGGCCCCTTAATAAGTTTGGTAAAGCTGGTTTATATTATGAGTCTATTAGTAGCGCTGGTTGGCCCCTTAATAAGTTTGGTAAAGCTGGTTTATATTATGAGTCTATTAGT

TATGAGGAACAAGATGCTATTTTTTCATTAACAAAGCGTAATATTCTCCCTACTATGACTTATGAGGAACAAGATGCTATTTTTTCATTAACAAAGCGTAATATTCTCCCTACTATGACT

CAGTTAAATCTTAAATACGCCATATCTGGTAAGGAACGCGCACGTACAGTGGGTGGCGTCCAGTTAAATCTTAAATACGCCATATCTGGTAAGGAACGCGCACGTACAGTGGGTGGCGTC

TCTTTATTAGCTACTATGACTACAAGACAGTTTCATCAGAAATGTCTGAAATCCATAGTATCTTTATTAGCTACTATGACTACAAGACAGTTTCATCAGAAATGTCTGAAATCCATAGTA

GCTACCAGAAATGCCACCGTTGTTATCGGCACTACCAAGTTTTATGGCGGGTGGGATAATGCTACCAGAAATGCCACCGTTGTTATCGGCACTACCAAGTTTTATGGCGGGTGGGATAAT

ATGTTAAAGAACCTGATGGCCGATGTTGATGATCCTAAATTGATGGGATGGGACTATCCTATGTTAAAGAACCTGATGGCCGATGTTGATGATCCTAAATTGATGGGATGGGACTATCCT

AAGTGTGATAGAGCTATGCCCTCAATGATTCGTATGTTGTCGGCTATGATCTTAGGTTCTAAGTGTGATAGAGCTATGCCCTCAATGATTCGTATGTTGTCGGCTATGATCTTAGGTTCT

AAGCATGTCACATGTTGTACGGCTAGTGATAAATTTTATAGACTTAGTAATGAGCTTGCTAAGCATGTCACATGTTGTACGGCTAGTGATAAATTTTATAGACTTAGTAATGAGCTTGCT

CAAGTTTTGACCGAGGTTGTTTATTCAAATGGTGGGTTTTATTTTAAACCTGGTGGTACACAAGTTTTGACCGAGGTTGTTTATTCAAATGGTGGGTTTTATTTTAAACCTGGTGGTACA

ACTTCTGGTGATGCAACTACAGCCTATGCCAATTCTGTCTTTAATATATTTCAGGCTGTAACTTCTGGTGATGCAACTACAGCCTATGCCAATTCTGTCTTTAATATATTTCAGGCTGTA

AGTTCTAACATTAATTGCGTTTTGAGCGTTAACTCGTCAAATTGCAATAATTTTAATGTTAGTTCTAACATTAATTGCGTTTTGAGCGTTAACTCGTCAAATTGCAATAATTTTAATGTT

AAGAAGTTACAGAGACAACTTTATGATAATTGCTATAGAAATAGTAATGTTGATGAATCTAAGAAGTTACAGAGACAACTTTATGATAATTGCTATAGAAATAGTAATGTTGATGAATCT

TTTGTGGATGACTTTTATGGTTATTTGCAAAAGCATTTTTCTATGATGATTCTTTCTGATTTTGTGGATGACTTTTATGGTTATTTGCAAAAGCATTTTTCTATGATGATTCTTTCTGAT

GATAGTGTTGTGTGCTATAATAAAACTTATGCTGGACTTGGTTACATTGCTGATATTAGTGATAGTGTTGTGTGCTATAATAAAACTTATGCTGGACTTGGTTACATTGCTGATATTAGT

GCTTTTAAAGCCACTTTGTATTATCAGAATGGTGTGTTTATGAGTACAGCTAAGTGTTGGGCTTTTAAAGCCACTTTGTATTATCAGAATGGTGTGTTTATGAGTACAGCTAAGTGTTGG

ACTGAGGAAGATCTTTCTATAGGACCTCATGAATTTTGCTCACAGCACACTATGCAGATTACTGAGGAAGATCTTTCTATAGGACCTCATGAATTTTGCTCACAGCACACTATGCAGATT

GTAGATGAAAATGGTAAGTATTATCTACCATATCCAGATCCTAGCCGTATTATTTCTGCTGTAGATGAAAATGGTAAGTATTATCTACCATATCCAGATCCTAGCCGTATTATTTCTGCT

GGTGTTTTTGTGGATGACATCACTAAGACTGATGCTGTCATTCTTTTGGAACGCTATGTTGGTGTTTTTGTGGATGACATCACTAAGACTGATGCTGTCATTCTTTTGGAACGCTATGTT

TCTCTGGCTATAGATGCCTACCCATTGTCTAAGCATCCTAAACCTGAGTACAGGAAGGTGTCTCTGGCTATAGATGCCTACCCATTGTCTAAGCATCCTAAACCTGAGTACAGGAAGGTG

TTTTACGCATTGTTAGACTGGGTCAAACATCTCAACAAGACTCTTAACGAAGGTGTTTTGTTTTACGCATTGTTAGACTGGGTCAAACATCTCAACAAGACTCTTAACGAAGGTGTTTTG

GAGTCTTTTTCTGTTACACTTTTAGATGAACATGAGTCTAAGTTTTGGGATGAAAGCTTTGAGTCTTTTTCTGTTACACTTTTAGATGAACATGAGTCTAAGTTTTGGGATGAAAGCTTT

TATGCTAGTATGTATGAGAAGTCTACAGTATTACAAGCTGCTGGTCTTTGTGTAGTATGTTATGCTAGTATGTATGAGAAGTCTACAGTATTACAAGCTGCTGGTCTTTGTGTAGTATGT

GGTTCTCAAACAGTTCTAAGATGCGGTGATTGTTTACGCAGACCGATGTTGTGCACTAAGGGTTCTCAAACAGTTCTAAGATGCGGTGATTGTTTACGCAGACCGATGTTGTGCACTAAG

TGCGCCTATGATCATGTGTTTGGCACTGATCATAAGTTCATTTTAGCTATTACACCATATTGCGCCTATGATCATGTGTTTGGCACTGATCATAAGTTCATTTTAGCTATTACACCATAT

GTGTGTAACACATCTGGCTGCAATGTAAATGACGTTACAAAACTGTATCTTGGAGGTTTGGTGTGTAACACATCTGGCTGCAATGTAAATGACGTTACAAAACTGTATCTTGGAGGTTTG

AATTATTACTGTGTAGACCACAAACCACATCTTTCATTCCCACTGTGTTCAGTTGGTAATAATTATTACTGTGTAGACCACAAACCACATCTTTCATTCCCACTGTGTTCAGTTGGTAAT

GTCTTTGGTTTGTACAAAAGTTCTGCTTTGGGTTCCATGGACATTGATGTCTTTAACAAAGTCTTTGGTTTGTACAAAAGTTCTGCTTTGGGTTCCATGGACATTGATGTCTTTAACAAA

CTTTCTACCTCTGATTGGTCTGACATTCGCGACTACAAGCTTGCTAATGATGCAAAAGAGCTTTCTACCTCTGATTGGTCTGACATTCGCGACTACAAGCTTGCTAATGATGCAAAAGAG

TCACTAAGGTTGTTTGCAGCTGAAACGGTCAAGGCTAAAGAGGAAAGTGTTAAGTCATCATCACTAAGGTTGTTTGCAGCTGAAACGGTCAAGGCTAAAGAGGAAAGTGTTAAGTCATCA

TACGCTTATGCTACCCTAAAGGAGATTGTAGGTCCTAAGGAACTTTTGCTCTTATGGGAATACGCTTATGCTACCCTAAAGGAGATTGTAGGTCCTAAGGAACTTTTGCTCTTATGGGAA

AGTGGAAAAGCCAAACCACCGTTAAACCGTAATTCTGTTTTTACATGCTTCCAAATTACAAGTGGAAAAGCCAAACCACCGTTAAACCGTAATTCTGTTTTTACATGCTTCCAAATTACA

AAAGACTCCAAGTTTCAAGTTGGTGAGTTTGTGTTTGAGAAAGTAGATTACGGTTCTGATAAAGACTCCAAGTTTCAAGTTGGTGAGTTTGTGTTTGAGAAAGTAGATTACGGTTCTGAT

ACGGTTACTTACAAATCCACTGCTACTACTAAGTTAGTACCAGGTATGTTGTTTATTTTGACGGTTACTTACAAATCCACTGCTACTACTAAGTTAGTACCAGGTATGTTGTTTATTTTG

ACTTCTCATAATGTTGCTCCACTTAGAGCGCCAACAATGGCAAACCAGGAGAAATATTCTACTTCTCATAATGTTGCTCCACTTAGAGCGCCAACAATGGCAAACCAGGAGAAATATTCT

ACCATTTACAAGTTGCACCCATCATTTAATGTTAGTGATGCTTATGCAAATCTTGTACCTACCATTTACAAGTTGCACCCATCATTTAATGTTAGTGATGCTTATGCAAATCTTGTACCT

TATTACCAACTTATTGGCAAACAGCGTATCACCACAATACAGGGTCCTCCTGGTAGTGGATATTACCAACTTATTGGCAAACAGCGTATCACCACAATACAGGGTCCTCCTGGTAGTGGA

AAATCGCATTGTTCTATTGGTATTGGTGTGTATTACCCTGGAGCGAGGATCGTGTTCACCAAATCGCATTGTTCTATTGGTATTGGTGTGTATTACCCTGGAGCGAGGATCGTGTTCACC

GCTTGTTCTCACGCTGCTGTTGATTCGCTCTGTGCAAAAGCTGTCACAGCCTATAGTGTTGCTTGTTCTCACGCTGCTGTTGATTCGCTCTGTGCAAAAGCTGTCACAGCCTATAGTGTT

GATAAGTGTACACGTATTATTCCTGCACGTGCCAGAGTTGAGTGTTATAGTGGTTTTAAAGATAAGTGTACACGTATTATTCCTGCACGTGCCAGAGTTGAGTGTTATAGTGGTTTTAAA

CCTAACAATAATAGTGCACAATACGTGTTTAGTACTGTTAATGCGTTACCTGAAGTTAATCCTAACAATAATAGTGCACAATACGTGTTTAGTACTGTTAATGCGTTACCTGAAGTTAAT

GCAGACATTGTTGTCGTGGATGAGGTGTCTATGTGCACTAACTATGACTTGTCTGTGATTGCAGACATTGTTGTCGTGGATGAGGTGTCTATGTGCACTAACTATGACTTGTCTGTGATT

AACCAGCGTATATCATATAAACACATTGTATATGTTGGTGATCCTCAACAGCTTCCAGCTAACCAGCGTATATCATATAAACACATTGTATATGTTGGTGATCCTCAACAGCTTCCAGCT

CCTAGAGTTCTTATCTCTAAAGGTGTTATGGAACCAATTGACTATAATGTTGTGACACAACCTAGAGTTCTTATCTCTAAAGGTGTTATGGAACCAATTGACTATAATGTTGTGACACAA

CGTATGTGTGCTATAGGACCCGATGTCTTTTTACACAAGTGTTACAGATGTCCTGCTGAACGTATGTGTGCTATAGGACCCGATGTCTTTTTACACAAGTGTTACAGATGTCCTGCTGAA

ATAGTTAACACTGTTTCAGAGCTTGTTTATGAAAACAAGTTTGTACCTGTCAAAGAAGCTATAGTTAACACTGTTTCAGAGCTTGTTTATGAAAACAAGTTTGTACCTGTCAAAGAAGCT

AGTAAGCAGTGCTTCAAAATCTTTGAACGCGGTAGTGTTCAGGTAGACAATGGCTCCAGTAGTAAGCAGTGCTTCAAAATCTTTGAACGCGGTAGTGTTCAGGTAGACAATGGCTCCAGT

ATAAATAGGCGTCAACTTGATGTTGTTAAGCGATTTATACATAAAAACTCCACATGGAGCATAAATAGGCGTCAACTTGATGTTGTTAAGCGATTTATACATAAAAACTCCACATGGAGC

AAGGCTGTGTTTATCTCACCTTACAATAGTCAAAATTATGTAGCTGCCAGGCTTTTAGGCAAGGCTGTGTTTATCTCACCTTACAATAGTCAAAATTATGTAGCTGCCAGGCTTTTAGGC

TTACAAACTCAGACAGTGGATTCTGCTCAAGGTAGTGAATATGACTATGTTATATTCGCATTACAAACTCAGACAGTGGATTCTGCTCAAGGTAGTGAATATGACTATGTTATATTCGCA

CAGACATCAGATACTGCTCATGCCTGTAATGCCAATCGTTTTAACGTTGCCATTACTAGACAGACATCAGATACTGCTCATGCCTGTAATGCCAATCGTTTTAACGTTGCCATTACTAGA

GCAAAGAAAGGTATTTTCTGTATTATGTCTGACAGAACTTTGTTTGATGCACTTAAGTTCGCAAAGAAAGGTATTTTCTGTATTATGTCTGACAGAACTTTGTTTGATGCACTTAAGTTC

TTTGAAATCACTATGACAGATTTACAGTCTGAAAGTAGTTGTGGTTTGTTTAAGGATTGTTTTGAAATCACTATGACAGATTTACAGTCTGAAAGTAGTTGTGGTTTGTTTAAGGATTGT

GCACGTAACCCTATTGATTTACCACCAAGTCATGCCACTACTTATTTGTCATTGTCTGATGCACGTAACCCTATTGATTTACCACCAAGTCATGCCACTACTTATTTGTCATTGTCTGAT

AGATTTAAGACTAGTGGTGACTTGGCTGTTCAAATAGGTAACAACAATGTTTGTACCTATAGATTTAAGACTAGTGGTGACTTGGCTGTTCAAATAGGTAACAACAATGTTTGTACCTAT

GAACATGTGATTTCATATATGGGTTTCAGGTTTGATGTTAGCATGCCTGGTAGTCATAGTGAACATGTGATTTCATATATGGGTTTCAGGTTTGATGTTAGCATGCCTGGTAGTCATAGT

TTGTTCTGTACTAGAGACTTTGCCATGCGTCATGTCAGAGGTTGGTTAGGAATGGATGTGTTGTTCTGTACTAGAGACTTTGCCATGCGTCATGTCAGAGGTTGGTTAGGAATGGATGTG

GAAGGTGCACATGTCACAGGTGACAATGTTGGCACTAATGTACCTCTACAAGTTGGTTTTGAAGGTGCACATGTCACAGGTGACAATGTTGGCACTAATGTACCTCTACAAGTTGGTTTT

TCCAATGGTGTTGATTTTGTAGCTCAACCTGAAGGTTGTGTTCTAACAAACACTGGCAGTTCCAATGGTGTTGATTTTGTAGCTCAACCTGAAGGTTGTGTTCTAACAAACACTGGCAGT

GTTGTAAAACCTGTTCGTGCTCGTGCACCACCTGGAGAACAATTTACTCACATTGTACCTGTTGTAAAACCTGTTCGTGCTCGTGCACCACCTGGAGAACAATTTACTCACATTGTACCT

CTGTTACGCAAGGGACAACCTTGGAGTGTGTTGAGAAAACGTATTGTTCAAATGATAGCACTGTTACGCAAGGGACAACCTTGGAGTGTGTTGAGAAAACGTATTGTTCAAATGATAGCA

GATTTTCTTGCTGGCTCATCTGATGTACTGGTGTTTGTACTTTGGGCTGGCGGTTTAGAGGATTTTCTTGCTGGCTCATCTGATGTACTGGTGTTTGTACTTTGGGCTGGCGGTTTAGAG

TTGACCACTATGCGTTATTTTGTTAAGATTGGAGCTGTTAAACATTGCCAATGTGGTACTTTGACCACTATGCGTTATTTTGTTAAGATTGGAGCTGTTAAACATTGCCAATGTGGTACT

GTTGCAACATGCTACAATTCTGTTAGTAATGACTATTGTTGCTTTAAACATGCATTGGGCGTTGCAACATGCTACAATTCTGTTAGTAATGACTATTGTTGCTTTAAACATGCATTGGGC

TGTGACTATGTTTATAATCCATATGTCATAGATATTCAACAATGGGGTTATGTTGGTTCATGTGACTATGTTTATAATCCATATGTCATAGATATTCAACAATGGGGTTATGTTGGTTCA

CTCTCCACTAATCACCATGCAATTTGTAATGTTCATAGAAATGAGCATGTTGCTTCTGGTCTCTCCACTAATCACCATGCAATTTGTAATGTTCATAGAAATGAGCATGTTGCTTCTGGT

GATGCTATTATGACTAGATGTTTGGCTGTGTATGACTGCTTTGTTAAGAATGTGGATTGGGATGCTATTATGACTAGATGTTTGGCTGTGTATGACTGCTTTGTTAAGAATGTGGATTGG

TCAATTACCTACCCTATGATAGCTAATGAAAATGCCATAAACAAGGGCGGTCGCACTGTGTCAATTACCTACCCTATGATAGCTAATGAAAATGCCATAAACAAGGGCGGTCGCACTGTG

CAGAGTCATATTATGCGTGCTGCTATTAAATTGTACAACCCTAAAGCAATCCATGACATTCAGAGTCATATTATGCGTGCTGCTATTAAATTGTACAACCCTAAAGCAATCCATGACATT

GGTAATCCTAAGGGTATTCGTTGTGCTGTAACTGATGCCAAGTGGTATTGTTATGACAAGGGTAATCCTAAGGGTATTCGTTGTGCTGTAACTGATGCCAAGTGGTATTGTTATGACAAG

AACCCTATTAATTCTAATGTGAAAACATTGGAGTATGATTACATGACACATGGCCAAATGAACCCTATTAATTCTAATGTGAAAACATTGGAGTATGATTACATGACACATGGCCAAATG

GATGGCTTGTGTTTGTTTTGGAATTGTAATGTGGATATGTACCCTGAATTCTCAATTGTTGATGGCTTGTGTTTGTTTTGGAATTGTAATGTGGATATGTACCCTGAATTCTCAATTGTT

TGCAGGTTTGACACACGTACACGATCTACATTGAACCTTGAAGGTGTAAATGGTGGGTCATGCAGGTTTGACACACGTACACGATCTACATTGAACCTTGAAGGTGTAAATGGTGGGTCA

TTGTATGTCAATAATCATGCATTTCACACTCCTGCTTATGATAAACGTGCTATGGCTAAATTGTATGTCAATAATCATGCATTTCACACTCCTGCTTATGATAAACGTGCTATGGCTAAA

TTGAAACCAGCACCGTTTTTCTACTATGACGACGGTTCATGTGAGGTTGTTCACGATCAATTGAAACCAGCACCGTTTTTCTACTATGACGACGGTTCATGTGAGGTTGTTCACGATCAA

GTTAACTATGTTCCTTTGAGAGCCACTAATTGCATTACCAAGTGTAATATTGGTGGTGCTGTTAACTATGTTCCTTTGAGAGCCACTAATTGCATTACCAAGTGTAATATTGGTGGTGCT

GTATGTTCTAAGCACGCTAATCTCTATAGAGCATATGTTGAGTCATATAACATTTTTACTGTATGTTCTAAGCACGCTAATCTCTATAGAGCATATGTTGAGTCATATAACATTTTTACT

CAAGCTGGTTTTAATATTTGGGTTCCTACCACGTTTGATTGTTATAATTTGTGGCAGACACAAGCTGGTTTTAATATTTGGGTTCCTACCACGTTTGATTGTTATAATTTGTGGCAGACA

TTCACAGAGGTCAATTTACAAGGTTTAGAGAACATTGCTTTTAACGTTGTTAATAAAGGTTTCACAGAGGTCAATTTACAAGGTTTAGAGAACATTGCTTTTAACGTTGTTAATAAAGGT

TCATTTGTTGGTGCTGATGGTGAATTACCAGTAGCCATTAGTGGTGATAAAGTGTTCGTATCATTTGTTGGTGCTGATGGTGAATTACCAGTAGCCATTAGTGGTGATAAAGTGTTCGTA

CGTGATGGTAACACTGATAATTTAGTCTTTGTTAACAAAACATCACTGCCTACAAACATACGTGATGGTAACACTGATAATTTAGTCTTTGTTAACAAAACATCACTGCCTACAAACATA

GCATTTGAACTTTTTGCTAAGAGGAAGGTTGGTTTAACACCACCTCTCAGTATTCTCAAAGCATTTGAACTTTTTGCTAAGAGGAAGGTTGGTTTAACACCACCTCTCAGTATTCTCAAA

AACCTTGGTGTTGTCGCCACATATAAGTTTGTCTTGTGGGATTATGAAGCTGAGCGTCCCAACCTTGGTGTTGTCGCCACATATAAGTTTGTCTTGTGGGATTATGAAGCTGAGCGTCCC

TTGACAAGCTTTACTAAGTCTGTTTGTGGTTATACAGACTTTGCAGAGGATGTTTGTACTTTGACAAGCTTTACTAAGTCTGTTTGTGGTTATACAGACTTTGCAGAGGATGTTTGTACT

TGTTACGATAATAGTATACAAGGTTCATACGAACGTTTTACTCTGTCAACTAATGCTGTGTGTTACGATAATAGTATACAAGGTTCATACGAACGTTTTACTCTGTCAACTAATGCTGTG

TTATTCTCTGCTACTGCTGTGAAAACAGGTGGTAAGAGTTTGCCGGCTATTAAATTGAATTTATTCTCTGCTACTGCTGTGAAAACAGGTGGTAAGAGTTTGCCGGCTATTAAATTGAAT

TTTGGAATGCTTAATGGTAATGCAATTGCTACTGTCAAATCAGAAGATGGTAACATAAAATTTGGAATGCTTAATGGTAATGCAATTGCTACTGTCAAATCAGAAGATGGTAACATAAAA

AATATTAACTGGTTTGTTTACGTACGCAAAGATGGCAAACCTGTTGATCATTATGATGGTAATATTAACTGGTTTGTTTACGTACGCAAAGATGGCAAACCTGTTGATCATTATGATGGT

TTTTATACCCAAGGTCGTAATTTACAAGACTTTTTGCCTCGCAGCACAATGGAAGAAGACTTTTATACCCAAGGTCGTAATTTACAAGACTTTTTGCCTCGCAGCACAATGGAAGAAGAC

TTTTTGAACATGGATATAGGCGTGTTTATTCAAAAGTATGGTCTAGAGGATTTCAACTTCTTTTTGAACATGGATATAGGCGTGTTTATTCAAAAGTATGGTCTAGAGGATTTCAACTTC

GAGCACGTTGTGTATGGTGATGTTTCAAAAACTACTCTAGGCGGTTTACACTTGTTGATTGAGCACGTTGTGTATGGTGATGTTTCAAAAACTACTCTAGGCGGTTTACACTTGTTGATT

TCACAAGTACGTCTGAGTAAAATGGGCATCTTAAAGGCAGAGGAGTTTGTGGCAGCATCTTCACAAGTACGTCTGAGTAAAATGGGCATCTTAAAGGCAGAGGAGTTTGTGGCAGCATCT

GACATAACACTCAAATGTTGTACTGTGACTTATCTTAATGATCCTAGTTCTAAGACTGTTGACATAACACTCAAATGTTGTACTGTGACTTATCTTAATGATCCTAGTTCTAAGACTGTT

TGTACTTACATGGATTTGTTGTTGGATGATTTTGTTTCTGTATTGAAGTCTTTGGATTTGTGTACTTACATGGATTTGTTGTTGGATGATTTTGTTTCTGTATTGAAGTCTTTGGATTTG

ACTGTTGTATCCAAGGTTCATGAGGTCATAATTGACAACAAACCATGGAGATGGATGCTAACTGTTGTATCCAAGGTTCATGAGGTCATAATTGACAACAAACCATGGAGATGGATGCTA

TGGTGTAAAGATAATGCCGTTGCTACATTCTATCCTCAGTTGCAGAGTGCAGAATGGAAATGGTGTAAAGATAATGCCGTTGCTACATTCTATCCTCAGTTGCAGAGTGCAGAATGGAAA

TGCGGGTATTCTATGCCTGGTATTTATAAGACACAACGTATGTGCTTAGAACCATGTAATTGCGGGTATTCTATGCCTGGTATTTATAAGACACAACGTATGTGCTTAGAACCATGTAAT

TTGTATAATTATGGTGCAGGTTTGAAGTTGCCCAGTGGCATTATGTTCAATGTTGTTAAATTGTATAATTATGGTGCAGGTTTGAAGTTGCCCAGTGGCATTATGTTCAATGTTGTTAAA

TACACTCAATTGTGTCAATATTTTAACAGTACCACGTTATGTGTTCCTCATAATATGAGATACACTCAATTGTGTCAATATTTTAACAGTACCACGTTATGTGTTCCTCATAATATGAGA

GTGTTACACTTGGGTGCTGGCTCTGATTATGGTGTTGCACCAGGAACTGCTGTTCTTAAAGTGTTACACTTGGGTGCTGGCTCTGATTATGGTGTTGCACCAGGAACTGCTGTTCTTAAA

AGGTGGTTGCCGCACGACGCAATTGTTGTTGACAACGATGTTGTTGACTATGTGAGTGACAGGTGGTTGCCGCACGACGCAATTGTTGTTGACAACGATGTTGTTGACTATGTGAGTGAC

GCTGATTTTAGTGTTACTGGTGATTGTGCAACCGTTTATTTGGAAGACAAGTTTGACTTGGCTGATTTTAGTGTTACTGGTGATTGTGCAACCGTTTATTTGGAAGACAAGTTTGACTTG

TTAATCTCTGATATGTACGATGGCAGGACAAAGGCAATTGATGGTGAAAATGTTTCGAAATTAATCTCTGATATGTACGATGGCAGGACAAAGGCAATTGATGGTGAAAATGTTTCGAAA

GAAGGATTTTTCACTTACATCTATGGTTTCATTTGTGAAAAACTTGCCATCGGAGGTTCGGAAGGATTTTTCACTTACATCTATGGTTTCATTTGTGAAAAACTTGCCATCGGAGGTTCG

ATTGCTATTAAAGTAACAGAGTATAGCTGGAATAAGAAATTGTATGAACTTGTACAAAGAATTGCTATTAAAGTAACAGAGTATAGCTGGAATAAGAAATTGTATGAACTTGTACAAAGA

TTTTCTTTTTGGACTATGTTTTGCACTTCTGTTAATACGTCATCATCAGAAGCCTTTGTTTTTTCTTTTTGGACTATGTTTTGCACTTCTGTTAATACGTCATCATCAGAAGCCTTTGTT

GTCGGAATTAACTATCTTGGTGATTTCGCACAAGGACCTTTTATAGATGGTAACATAATAGTCGGAATTAACTATCTTGGTGATTTCGCACAAGGACCTTTTATAGATGGTAACATAATA

CACGCAAATTATGTATTTTGGCGTAACTCCACTGTTATGAGTTTGTCCTACAACTCTGTTCACGCAAATTATGTATTTTGGCGTAACTCCACTGTTATGAGTTTGTCCTACAACTCTGTT

TTAGACCTGAGTAAATTTAATTGCAAACACAAAGCGACTGTTGTTGTGCAATTAAAGGATTTAGACCTGAGTAAATTTAATTGCAAACACAAAGCGACTGTTGTTGTGCAATTAAAGGAT

AGTGATATTAATGAAATGGTGCTTAGTCTTGTTAGGAGTGGTAAGTTGCTTGTAAGGGGTAGTGATATTAATGAAATGGTGCTTAGTCTTGTTAGGAGTGGTAAGTTGCTTGTAAGGGGT

AATGGCAAGTGTTTGAGTTTTAGTAATCATTTAGTCTCAACTAAATAAAATGTTTGTTTTAATGGCAAGTGTTTGAGTTTTAGTAATCATTTAGTCTCAACTAAATAAAATGTTTGTTTT

GCTTGTTGCATATGCCTTGTTGCATATTGCTGGTTGTCAAACTACAAATGGGCTGAACACGCTTGTTGCATATGCCTTGTTGCATATTGCTGGTTGTCAAACTACAAATGGGCTGAACAC

TAGTTACTCTGTTTGCAACGGCTGTGTTGGTTATTCAGAAAATGTATTTGCTGTTGAGAGTAGTTACTCTGTTTGCAACGGCTGTGTTGGTTATTCAGAAAATGTATTTGCTGTTGAGAG

TGGTGGTTATATACCCTCCGACTTTGCATTCAATAATTGGTTCCTTCTAACTAATACCTCTGGTGGTTATATACCCTCCGACTTTGCATTCAATAATTGGTTCCTTCTAACTAATACCTC

ATCTGTTGTAGATGGTGTTGTGAGGAGTTTTCAGCCTTTGTTGCTTAATTGCTTATGGTCATCTGTTGTAGATGGTGTTGTGAGGAGTTTTCAGCCTTTGTTGCTTAATTGCTTATGGTC

TGTTTCTGGCTTGCGGTTTACTACTGGTTTTGTCTATTTTAATGGTACTGGGAGAGGTGATGTTTCTGGCTTGCGGTTTACTACTGGTTTTGTCTATTTTAATGGTACTGGGAGAGGTGA

TTGTAAAGGTTTTTCCTCAGATGTTTTGTCTGATGTCATACGTTACAACCTCAATTTTGATTGTAAAGGTTTTTCCTCAGATGTTTTGTCTGATGTCATACGTTACAACCTCAATTTTGA

AGAAAACCTTAGACGTGGAACCATTTTGTTTAAAACATCTTATGGTGTTGTTGTGTTTTAAGAAAACCTTAGACGTGGAACCATTTTGTTTAAAACATCTTATGGTGTTGTTGTGTTTTA

TTGTACCAACAACACTTTAGTTTCAGGTGATGCTCACATACCATTTGGTACAGTTTTGGGTTGTACCAACAACACTTTAGTTTCAGGTGATGCTCACATACCATTTGGTACAGTTTTGGG

CAATTTTTATTGCTTTGTAAATACTACTATTGGCAATGAAACTACGTCTGCTTTTGTGGGCAATTTTTATTGCTTTGTAAATACTACTATTGGCAATGAAACTACGTCTGCTTTTGTGGG

TGCACTACCTAAGACAGTTCGTGAGTTTGTTATTTCACGCACAGGACATTTTTATATTAATGCACTACCTAAGACAGTTCGTGAGTTTGTTATTTCACGCACAGGACATTTTTATATTAA

TGGCTATCGCTATTTCACTTTAGGTAATGTAGAAGCCGTTAATTTCAATGTCACTACTGCTGGCTATCGCTATTTCACTTTAGGTAATGTAGAAGCCGTTAATTTCAATGTCACTACTGC

AGAAACCACTGATTTTTGCACTGTTGCGTTAGCTTCTTATGCTGACGTTTTGGTTAATGTAGAAACCACTGATTTTTGCACTGTTGCGTTAGCTTCTTATGCTGACGTTTTGGTTAATGT

GTCACAAACCTCTATTGCTAATATAATTTATTGCAACTCTGTTATTAACAGACTGAGATGGTCACAAACCTCTATTGCTAATATAATTTATTGCAACTCTGTTATTAACAGACTGAGATG

TGACCAGTTGTCCTTTGATGTACCAGATGGTTTTTATTCTACAAGCCCTATTCAATCCGTTGACCAGTTGTCCTTTGATGTACCAGATGGTTTTTATTCTACAAGCCCTATTCAATCCGT

TGAGCTACCTGTGTCTATTGTGTCGCTACCTGTTTATCATAAACATACGTTTATTGTGTTTGAGCTACCTGTGTCTATTGTGTCGCTACCTGTTTATCATAAACATACGTTTATTGTGTT

GTACGTTGACTTCAAACCTCAGAGTGGCGGTGGCAAGTGCTTTAACTGTTATCCTGCTGGGTACGTTGACTTCAAACCTCAGAGTGGCGGTGGCAAGTGCTTTAACTGTTATCCTGCTGG

TGTTAATATTACACTGGCCAATTTTAATGAAACTAAAGGGCCTTTGTGTGTTGACACATCTGTTAATATTACACTGGCCAATTTTAATGAAACTAAAGGGCCTTTGTGTGTTGACACATC

ACACTTCACTACCAAATACGTTGCTGTTTATGCCAATGTTGGTAGGTGGAGTGCTAGTATACACTTCACTACCAAATACGTTGCTGTTTATGCCAATGTTGGTAGGTGGAGTGCTAGTAT

TAACACGGGAAATTGCCCTTTTTCTTTTGGCAAAGTTAATAACTTTGTTAAATTTGGCAGTAACACGGGAAATTGCCCTTTTTCTTTTGGCAAAGTTAATAACTTTGTTAAATTTGGCAG

TGTATGTTTTTCGCTAAAGGATATACCCGGTGGTTGCGCAATGCCTATAGTGGCTAATTGTGTATGTTTTTCGCTAAAGGATATACCCGGTGGTTGCGCAATGCCTATAGTGGCTAATTG

GGCTTATAGTAAGTACTATACTATAGGCTCATTGTATGTTTCTTGGAGTGATGGTGATGGGGCTTATAGTAAGTACTATACTATAGGCTCATTGTATGTTTCTTGGAGTGATGGTGATGG

AATTACTGGCGTCCCACAACCTGTTGAGGGTGTTAGTTCCTTTATGAATGTTACATTGGAAATTACTGGCGTCCCACAACCTGTTGAGGGTGTTAGTTCCTTTATGAATGTTACATTGGA

CAAATGTACTAAATATAATATTTATGATGTATCTGGTGTGGGTGTTATTCGCGTTAGCAACAAATGTACTAAATATAATATTTATGATGTATCTGGTGTGGGTGTTATTCGCGTTAGCAA

TGACACCTTTCTTAATGGAATTACGTACACATCAACTTCAGGTAACCTTCTGGGTTTTAATGACACCTTTCTTAATGGAATTACGTACACATCAACTTCAGGTAACCTTCTGGGTTTTAA

AGATGTTACTAAGGGCACCATCTACTCTATCACTCCTTGTAACCCACCAGATCAGCTTGTAGATGTTACTAAGGGCACCATCTACTCTATCACTCCTTGTAACCCACCAGATCAGCTTGT

TGTTTATCAGCAAGCTGTTGTTGGTGCTATGTTGTCTGAAAATTTTACTAGTTACGGCTTTGTTTATCAGCAAGCTGTTGTTGGTGCTATGTTGTCTGAAAATTTTACTAGTTACGGCTT

TTCTAATGTTGTAGAACTGCCGAAATTTTTCTATGCGTCCAATGGCACTTATAATTGCACTTCTAATGTTGTAGAACTGCCGAAATTTTTCTATGCGTCCAATGGCACTTATAATTGCAC

AGACGCTGTTTTAACTTATTCTAGTTTTGGCGTTTGTGCAGATGGTTCTATAATTGCTGTAGACGCTGTTTTAACTTATTCTAGTTTTGGCGTTTGTGCAGATGGTTCTATAATTGCTGT

TCAACCACGTAATGTTTCATATGATAGTGTTTCAGCTATCGTCACAGCTAATTTGTCTATTCAACCACGTAATGTTTCATATGATAGTGTTTCAGCTATCGTCACAGCTAATTTGTCTAT

ACCTTCCAATTGGACCACTTCGGTCCAGGTTGAGTATTTACAAATTACAAGTACACCTATACCTTCCAATTGGACCACTTCGGTCCAGGTTGAGTATTTACAAATTACAAGTACACCTAT

CGTAGTTGATTGCTCCACTTATGTTTGCAATGGTAATGTGCGCTGTGTTGAATTGCTTAACGTAGTTGATTGCTCCACTTATGTTTGCAATGGTAATGTGCGCTGTGTTGAATTGCTTAA

GCAGTATACTTCTGCTTGTAAAACCATAGAAGACGCCTTAAGAAATAGCGCCAGGCTGGAGCAGTATACTTCTGCTTGTAAAACCATAGAAGACGCCTTAAGAAATAGCGCCAGGCTGGA

GTCTGCAGATGTTAGTGAGATGCTCACTTTTGACAAGAAAGCGTTTACACTTGCTAATGTGTCTGCAGATGTTAGTGAGATGCTCACTTTTGACAAGAAAGCGTTTACACTTGCTAATGT

TAGTAGTTTTGGTGACTACAACCTTAGCAGCGTCATACCTAGCTTGCCCACAAGTGGTAGTAGTAGTTTTGGTGACTACAACCTTAGCAGCGTCATACCTAGCTTGCCCACAAGTGGTAG

TAGAGTGGCTGGTCGCAGTGCCATAGAAGACATACTTTTTAGCAAACTTGTTACTTCTGGTAGAGTGGCTGGTCGCAGTGCCATAGAAGACATACTTTTTAGCAAACTTGTTACTTCTGG

ACTTGGCACTGTGGACGCAGACTACAAAAATTGCACTAAGGGTCTTTCCATTGCTGACTTACTTGGCACTGTGGACGCAGACTACAAAAATTGCACTAAGGGTCTTTCCATTGCTGACTT

GGCTTGTGCTCAATATTATAATGGCATTATGGTTTTGCCTGGCGTCGCTGATGCTGAACGGGCTTGTGCTCAATATTATAATGGCATTATGGTTTTGCCTGGCGTCGCTGATGCTGAACG

AATGGCCATGTATACAGGTTCTTTAATTGGTGGAATTGCTTTAGGAGGTCTAACATCAGCAATGGCCATGTATACAGGTTCTTTAATTGGTGGAATTGCTTTAGGAGGTCTAACATCAGC

CGTTTCAATACCATTTTCATTAGCAATTCAGGCACGTTTAAATTATGTTGCATTGCAGACCGTTTCAATACCATTTTCATTAGCAATTCAGGCACGTTTAAATTATGTTGCATTGCAGAC

TGATGTTTTACAAGAAAATCAGAAAATTCTTGCTGCATCTTTTAACAAAGCAATGACCAATGATGTTTTACAAGAAAATCAGAAAATTCTTGCTGCATCTTTTAACAAAGCAATGACCAA

CATAGTAGATGCCTTTACTGGTGTTAATGATGCTATTACACAAACTTCACAAGCCCTACACATAGTAGATGCCTTTACTGGTGTTAATGATGCTATTACACAAACTTCACAAGCCCTACA

AACAGTTGCTACTGCACTTAACAAGATCCAGGATGTTGTTAATCAACAAGGCAACTTATTAACAGTTGCTACTGCACTTAACAAGATCCAGGATGTTGTTAATCAACAAGGCAACTTATT

GAACCATTTAACTTCTCAGTTGAGGCAGAATTTTCAAGCTATCTCTAGCTCTATTCAGGCGAACCATTTAACTTCTCAGTTGAGGCAGAATTTTCAAGCTATCTCTAGCTCTATTCAGGC

TATCTATGACAGACTTGACACTATTCAGGCTGATCAACAAGTAGATAGGCTGATTACTGGTATCTATGACAGACTTGACACTATTCAGGCTGATCAACAAGTAGATAGGCTGATTACTGG

TAGATTGGCTGCTTTGAATGTATTCGTTTCTCATACATTGACTAAGTACACTGAAGTTCGTAGATTGGCTGCTTTGAATGTATTCGTTTCTCATACATTGACTAAGTACACTGAAGTTCG

TGCTTCCAGACAGCTTGCACAACAAAAAGTGAATGAGTGTGTCAAATCCCAGTCTAAGCGTGCTTCCAGACAGCTTGCACAACAAAAAGTGAATGAGTGTGTCAAATCCCAGTCTAAGCG

TTATGGCTTCTGTGGAAATGGCACTCACATTTTCTCAATTGTTAATGCTGCTCCTGAGGGTTATGGCTTCTGTGGAAATGGCACTCACATTTTCTCAATTGTTAATGCTGCTCCTGAGGG

GCTTGTTTTTCTCCACACTGTCTTGTTGCCGACACAATATAAGGATGTTGAAGCGTGGTCGCTTGTTTTTCTCCACACTGTCTTGTTGCCGACACAATATAAGGATGTTGAAGCGTGGTC

TGGGTTGTGCGTTGATGGTACAAACGGTTATGTGTTGCGACAACCTAATCTTGCTCTTTATGGGTTGTGCGTTGATGGTACAAACGGTTATGTGTTGCGACAACCTAATCTTGCTCTTTA

CAAAGAAGGCAATTATTATAGAATCACATCTCGCATAATGTTTGAACCACGTATTCCTACCAAAGAAGGCAATTATTATAGAATCACATCTCGCATAATGTTTGAACCACGTATTCCTAC

CATGGCAGATTTTGTTCAAATTGAAAATTGCAATGTCACATTTGTTAACATTTCTCGCTCCATGGCAGATTTTGTTCAAATTGAAAATTGCAATGTCACATTTGTTAACATTTCTCGCTC

TGAGTTGCAAACCATTGTGCCAGAGTATATTGATGTTAATAAGACGCTGCAAGAATTAAGTGAGTTGCAAACCATTGTGCCAGAGTATATTGATGTTAATAAGACGCTGCAAGAATTAAG

TTACAAATTGCCAAATTACACTGTTCCAGACCTAGTTGTCGAACAGTACAACCAGACTATTTACAAATTGCCAAATTACACTGTTCCAGACCTAGTTGTCGAACAGTACAACCAGACTAT

TTTGAATTTGACCAGTGAAATTAGCACCCTTGAAAATAAATCTGCGGAGCTTAATTACACTTTGAATTTGACCAGTGAAATTAGCACCCTTGAAAATAAATCTGCGGAGCTTAATTACAC

TGTTCAAAAATTGCAAACTCTGATTGACAACATAAATAGCACATTAGTCGACTTAAAGTGTGTTCAAAAATTGCAAACTCTGATTGACAACATAAATAGCACATTAGTCGACTTAAAGTG

GCTCAACCGGGTTGAGACTTACATCAAGTGGCCGTGGTGGGTGTGGTTGTGCATTTCAGTGCTCAACCGGGTTGAGACTTACATCAAGTGGCCGTGGTGGGTGTGGTTGTGCATTTCAGT

CGTGCTCATCTTTGTGGTGAGTATGTTGCTATTATGTTGTTGTTCTACTGGTTGCTGTGGCGTGCTCATCTTTGTGGTGAGTATGTTGCTATTATGTTGTTGTTCTACTGGTTGCTGTGG

CTTCTTTAGTTGTTTTGCATCTTCTATTAGAGGTTGTTGTGAATCAACTAAACTTCCTTACTTCTTTAGTTGTTTTGCATCTTCTATTAGAGGTTGTTGTGAATCAACTAAACTTCCTTA

TTACGACGTTGAAAAGATCCACATACAGTAATGGCTCTAGGTTTGTTCACATTGCAACTTTTACGACGTTGAAAAGATCCACATACAGTAATGGCTCTAGGTTTGTTCACATTGCAACTT

GTGTCTGCTGTTAATCAATCGCTTAGCAATGCGAAAGTTAGTGCTGAAGTTTCACGACAGGTGTCTGCTGTTAATCAATCGCTTAGCAATGCGAAAGTTAGTGCTGAAGTTTCACGACAG

GTTATCCAAGACGTGAAAGATGGCACTGTTACCTTCAACTTGCTAGCGTATACACTAATGGTTATCCAAGACGTGAAAGATGGCACTGTTACCTTCAACTTGCTAGCGTATACACTAATG

AGCCTCTTTGTTGTGTATTTTGCTTTATTTAAAGCAAGATCACACCGTGGCAGAGCTGCTAGCCTCTTTGTTGTGTATTTTGCTTTATTTAAAGCAAGATCACACCGTGGCAGAGCTGCT

CTTATAGTGTTTAAAATTCTAATCCTTTTCGTTTATGTGCCATTGCTGTATTGGTCTCAACTTATAGTGTTTAAAATTCTAATCCTTTTCGTTTATGTGCCATTGCTGTATTGGTCTCAA

GCATATATTGACGCAACTTTGATTGCTGTAATTTTGCTTGGAAGATTTTTCCATACAGCTGCATATATTGACGCAACTTTGATTGCTGTAATTTTGCTTGGAAGATTTTTCCATACAGCT

TGGCACTGCTGGCTCTACAAGACATGGGATTTCATTGTCTTCAATGTAACCACACTTTGCTGGCACTGCTGGCTCTACAAGACATGGGATTTCATTGTCTTCAATGTAACCACACTTTGC

TATGCAAGGTAAGTGTTGGTTTCTTGAAAATAAGGCTCTGAAACCATTCGTTTGTTTTTATATGCAAGGTAAGTGTTGGTTTCTTGAAAATAAGGCTCTGAAACCATTCGTTTGTTTTTA

CGGAGGGGATCAATTCCTTTACATAGGCGACAGAATTGTTTCTTATCTCTCAACTAACGACGGAGGGGATCAATTCCTTTACATAGGCGACAGAATTGTTTCTTATCTCTCAACTAACGA

CTTGTACGTTGCTCTTAGAGGACGTATTGATAAAGACCTCAGCCTTTCTAGAAAGGTTGACTTGTACGTTGCTCTTAGAGGACGTATTGATAAAGACCTCAGCCTTTCTAGAAAGGTTGA

GTTATATAACAGTGAATGTGTATACTTGTTTTGTGAACACCCAGCTGTTGGAATAGTCAAGTTATATAACAGTGAATGTGTATACTTGTTTTGTGAACACCCAGCTGTTGGAATAGTCAA

CACAGATTTCAAATTAGAAATCCACTAAGATGTTCCTTAAGCTAGTGGATGATCATGCTTCACAGATTTCAAATTAGAAATCCACTAAGATGTTCCTTAAGCTAGTGGATGATCATGCTT

TGGTTGTTAATGTACTACTCTGGTGTGTGGTGCTTATAGTGATACTACTAGTGTGTATTATGGTTGTTAATGTACTACTCTGGTGTGTGGTGCTTATAGTGATACTACTAGTGTGTATTA

CAATAATTAAACTAACTAAGCTTTGTTTCACTTGCCATATGTTTTGTAATAGAACAGTTTCAATAATTAAACTAACTAAGCTTTGTTTCACTTGCCATATGTTTTGTAATAGAACAGTTT

ATGGCCCCATTAAAAATGTGTACCACATTTACCAATCATATATGCACATAGACCCTTTCCATGGCCCCATTAAAAATGTGTACCACATTTACCAATCATATATGCACATAGACCCTTTCC

CTAAACGAGTTATTGATTTCTAAACTAAACGACAATGTCAAATGACAATTGTACGGGTGACTAAACGAGTTATTGATTTCTAAACTAAACGACAATGTCAAATGACAATTGTACGGGTGA

CATTGTCACCCATTTGAAGAAGTGGAATTTTGGTTGGAATGTTATTCTAACCATATTCATCATTGTCACCCATTTGAAGAAGTGGAATTTTGGTTGGAATGTTATTCTAACCATATTCAT

TGTTATTCTTCAGTTTGGACACTATAAATACTCCAGATTGTTTTATGGTTTGAAGATGCTTGTTATTCTTCAGTTTGGACACTATAAATACTCCAGATTGTTTTATGGTTTGAAGATGCT

TGTACTGTGGCTTCTTTGGCCACTCGTACTTGCTTTGTCAATCTTTGACACCTGGGCTAATGTACTGTGGCTTCTTTGGCCACTCGTACTTGCTTTGTCAATCTTTGACACCTGGGCTAA

TTGGGATTCTAATTGGGCCTTTGTTGCATTTAGCTTTCTTATGGCCGTATCAACACTCGTTTGGGATTCTAATTGGGCCTTTGTTGCATTTAGCTTTCTTATGGCCGTATCAACACTCGT

TATGTGGGTGATGTACTTCGCAAACAGTTTCAGACTTTTCCGACGTGCTCGAACTTTTTGTATGTGGGTGATGTACTTCGCAAACAGTTTCAGACTTTTCCGACGTGCTCGAACTTTTTG

GGCATGGAATCCTGAGGTCAATGCAATCACTGTCACAACCGTGTTGGGACAGACATACTAGGCATGGAATCCTGAGGTCAATGCAATCACTGTCACAACCGTGTTGGGACAGACATACTA

TCAACCCATTCAACAAGCTCCAACAGGCATTACTGTGACCTTGCTGAGCGGCGTGCTTTATCAACCCATTCAACAAGCTCCAACAGGCATTACTGTGACCTTGCTGAGCGGCGTGCTTTA

CGTTGACGGACATAGATTGGCTTCAGGTGTTCAGGTTCATAACCTACCTGAATACATGACCGTTGACGGACATAGATTGGCTTCAGGTGTTCAGGTTCATAACCTACCTGAATACATGAC

AGTTGCCGTGCCGAGCACTACTATAATTTATAGTAGAGTCGGAAGGTCCGTAAATTCACAAGTTGCCGTGCCGAGCACTACTATAATTTATAGTAGAGTCGGAAGGTCCGTAAATTCACA

AAATAGCACAGGCTGGGTTTTCTACGTACGAGTAAAACACGGTGATTTTTCTGCAGTGAGAAATAGCACAGGCTGGGTTTTCTACGTACGAGTAAAACACGGTGATTTTTCTGCAGTGAG

CTCTCCCATGAGCAACATGACAGAAAACGAAAGATTGCTTCATTTTTCCTAAACTGAACGCTCTCCCATGAGCAACATGACAGAAAACGAAAGATTGCTTCATTTTTCCTAAACTGAACG

AAAAGATGGCTACAGTCAAATGGGCTGATGCATCTGAACCACAACGTGGTCGTCAGGGTAAAAAGATGGCTACAGTCAAATGGGCTGATGCATCTGAACCACAACGTGGTCGTCAGGGTA

GAATACCTTATTCTCTTTATAGCCCTTTGCTTGTTGATAGTGAACAACCTTGGAAGGTGAGAATACCTTATTCTCTTTATAGCCCTTTGCTTGTTGATAGTGAACAACCTTGGAAGGTGA

TACCTCGTAATTTGGTACCCATCAACAAGAAAGACAAAAATAAGCTTATAGGCTATTGGATACCTCGTAATTTGGTACCCATCAACAAGAAAGACAAAAATAAGCTTATAGGCTATTGGA

ATGTTCAAAAACGTTTCAGAACTAGAAAGGGCAAACGGGTGGATTTGTCACCCAAGCTGCATGTTCAAAAACGTTTCAGAACTAGAAAGGGCAAACGGGTGGATTTGTCACCCAAGCTGC

ATTTTTATTATCTTGGCACAGGACCCCATAAAGATGCAAAATTTAGAGAGCGTGTTGAAGATTTTTATTATCTTGGCACAGGACCCCATAAAGATGCAAAATTTAGAGAGCGTGTTGAAG

GTGTCGTCTGGGTTGCTGTTGATGGTGCTAAAACTGAACCTACAGGTTACGGTGTTAGGCGTGTCGTCTGGGTTGCTGTTGATGGTGCTAAAACTGAACCTACAGGTTACGGTGTTAGGC

GCAAGAATTCAGAACCAGAGATACCACACTTCAATCAAAAGCTCCCAAATGGTGTTACTGGCAAGAATTCAGAACCAGAGATACCACACTTCAATCAAAAGCTCCCAAATGGTGTTACTG

TTGTTGAAGAACCTGACTCCCGTGCTCCTTCCCGGTCTCAGTCGAGGTCGCAGAGTCGCGTTGTTGAAGAACCTGACTCCCGTGCTCCTTCCCGGTCTCAGTCGAGGTCGCAGAGTCGCG

GTCGTGGTGAATCCAAACCTCAATCTCGGAATCCTTCAAGTGACAGAAACCATAACAGTCGTCGTGGTGAATCCAAACCTCAATCTCGGAATCCTTCAAGTGACAGAAACCATAACAGTC

AGGATGACATCATGAAGGCAGTTGCTGCGGCTCTTAAATCTTTAGGTTTTGACAAGCCTCAGGATGACATCATGAAGGCAGTTGCTGCGGCTCTTAAATCTTTAGGTTTTGACAAGCCTC

AGGAAAAAGATAAAAAGTCAGCGAAAACGGGTACTCCTAAGCCTTCTCGTAATCAGAGTCAGGAAAAAGATAAAAAGTCAGCGAAAACGGGTACTCCTAAGCCTTCTCGTAATCAGAGTC

CTGCTTCTTCTCAAACTTCTGCCAAGAGTCTTGCTCGTTCTCAGAGTTCTGAAACAAAAGCTGCTTCTTCTCAAACTTCTGCCAAGAGTCTTGCTCGTTCTCAGAGTTCTGAAACAAAAG

AACAAAAGCATGAAATGCAAAAGCCACGGTGGAAAAGACAGCCTAATGATGATGTGACATAACAAAAGCATGAAATGCAAAAGCCACGGTGGAAAAGACAGCCTAATGATGATGTGACAT

CTAATGTCACACAATGTTTTGGCCCCAGAGACCTTGACCACAACTTTGGAAGTGCAGGTGCTAATGTCACACAATGTTTTGGCCCCAGAGACCTTGACCACAACTTTGGAAGTGCAGGTG

TTGTGGCCAATGGTGTTAAAGCTAAAGGCTATCCACAATTTGCTGAGCTTGTGCCGTCAATTGTGGCCAATGGTGTTAAAGCTAAAGGCTATCCACAATTTGCTGAGCTTGTGCCGTCAA

CAGCTGCTATGCTGTTTGATAGTCACATTGTTTCCAAAGAGTCAGGCAACACTGTGGTCTCAGCTGCTATGCTGTTTGATAGTCACATTGTTTCCAAAGAGTCAGGCAACACTGTGGTCT

TGACTTTCACTACTAGAGTGACTGTGCCCAAAGACCATCCACACTTGGGTAAGTTTCTTGTGACTTTCACTACTAGAGTGACTGTGCCCAAAGACCATCCACACTTGGGTAAGTTTCTTG

AGGAGTTAAATGCATTCACTAGAGAAATGCAACAACATCCTCTTCTTAACCCTAGTGCACAGGAGTTAAATGCATTCACTAGAGAAATGCAACAACATCCTCTTCTTAACCCTAGTGCAC

TAGAATTCAACCCATCTCAAACTTCACCTGCAACTGCTGAACCAGTGCGTGATGAAGTTTTAGAATTCAACCCATCTCAAACTTCACCTGCAACTGCTGAACCAGTGCGTGATGAAGTTT

CTATTGAAACTGACATAATTGATGAAGTAAACTAAACATGCCACTGTGTTGTTTGAAATTCTATTGAAACTGACATAATTGATGAAGTAAACTAAACATGCCACTGTGTTGTTTGAAATT

CAGGCTTTAGTTGGAATTTTGCTTTTGTTCTTGCTTTTATTATCTTTCTTTTGCCTGTTTCAGGCTTTAGTTGGAATTTTGCTTTTGTTCTTGCTTTTATTATCTTTCTTTTGCCTGTTT

TTAGAGAGATTTGGCGCCTTGGTGCCGTAGATGAATACATTGCTTTTCTCTGATCTATGTTTAGAGAGATTTGGCGCCTTGGTGCCGTAGATGAATACATTGCTTTTCTCTGATCTATGT

ATGATGGTACGATCAGAGCTGCTTTTAATTAACATGATCCCTTGCTTTGGCTTGACAAGGATGATGGTACGATCAGAGCTGCTTTTAATTAACATGATCCCTTGCTTTGGCTTGACAAGG

ATCTAGTCTTATACACAATGGTAAGCCAGTGGTAGTAAAGGTATAAGAAATTTGCTACTAATCTAGTCTTATACACAATGGTAAGCCAGTGGTAGTAAAGGTATAAGAAATTTGCTACTA

TGTTACTGAACCTAGGTGAACGCTAGTATAACTCATTACAAATGTGCTGGAGTAATCAAATGTTACTGAACCTAGGTGAACGCTAGTATAACTCATTACAAATGTGCTGGAGTAATCAAA

GATCGCATTGACGAGCCAACAATGGAAGAGCCAGTCATTTGTCTTGAGACCTATCTAGTTGATCGCATTGACGAGCCAACAATGGAAGAGCCAGTCATTTGTCTTGAGACCTATCTAGTT

AGTAACTGCTAATGGAACGGTTTCGATATGGATACACAAAAAAAAAAAAAAAAAAAAAAAAGTAACTGCTAATGGAACGGTTTCGATATGGATACACAAAAAAAAAAAAAAAAAAAAAAA

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

<110> KRIBB <120> Human alphacoronavirus universal primer sets for whole genome amplification method and diagnosis kit <130> M18-5701 <160> 88 <170> KoPatentIn 3.0 <210> 1 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> 43 Forward primer <400> 1 tagactttgt gtctactyyt ctc 23 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 5385 Reverse primer <400> 2 gcattaccca acaattattt 20 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 4358 Forward primer <220> <221> modified_base <222> (18) <223> i <400> 3 tttaatgttg twggkccncg 20 <210> 4 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> 9077 Reverse primer <400> 4 gcataacaag ccacgta 17 <210> 5 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> 8201 Forward primer <400> 5 ggtggtraya atgtttattg yta 23 <210> 6 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> 11113 Reverse primer <400> 6 agccaaaatc acttgtta 18 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 9077 Forward primer <400> 7 taycgytgyg cttgttatgc 20 <210> 8 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> 13477 Reverse primer <400> 8 ggacaccatc ataaaac 17 <210> 9 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> 12255 Forward primer <400> 9 ctggtarygg tcargctat 19 <210> 10 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> 15343 Reverse primer <400> 10 cattagtcac aacatgg 17 <210> 11 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 13477 Forward primer <400> 11 gtktttatwg atggtgtwcc 20 <210> 12 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> 17266 Reverse primer <400> 12 aacaaacttg actccagg 18 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 15343 Forward primer <400> 13 cckatgttgt gyactaartg 20 <210> 14 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> 20047 Reverse primer <400> 14 ctccatctac atatcga 17 <210> 15 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 17266 Forward primer <400> 15 cctggyagtc ayagtttgtt 20 <210> 16 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> 22457 Reverse primer <400> 16 taaggctctt catgttttac a 21 <210> 17 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> 19333 Forward primer <400> 17 tatggtgatg tktcwaaaac tac 23 <210> 18 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> 24066 Reverse primer <400> 18 tctaataagg aagtttagtt ga 22 <210> 19 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> 22457 Forward primer <400> 19 tgtaaaacya twgaagaygc ctta 24 <210> 20 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> polyT Reverse primer <400> 20 tttttttttt tttgtgtatc catatc 26 <210> 21 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Alpha13F primer <400> 21 ttwtctatct ayagatagar aa 22 <210> 22 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Alpha41F primer <400> 22 tttagacttt gtgtctacty ytctc 25 <210> 23 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha43F primer <400> 23 tagactttgt gtctactyyt ctc 23 <210> 24 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha62F primer <400> 24 tctcaactaa acgaaatttt t 21 <210> 25 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Alpha252F primer <400> 25 aagcaamgtt ytgtckttgt gg 22 <210> 26 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Alpha950F primer <220> <221> modified_base <222> (6) <223> i <220> <221> modified_base <222> (9) <223> i <220> <221> modified_base <222> (18) <223> i <400> 26 ggttcnccnk ttatkacnaa tggt 24 <210> 27 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Alpha1421F primer <400> 27 ggtgtyatwg ayattagt 18 <210> 28 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha2981F primer <400> 28 ggtggyaarr twtcwttytc tga 23 <210> 29 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Alpha3940F primer <220> <221> modified_base <222> (4) <223> i <400> 29 trtngatggt ttyggtgt 18 <210> 30 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha4358F primer <220> <221> modified_base <222> (18) <223> i <400> 30 tttaatgttg twggkccncg 20 <210> 31 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha4904F primer <400> 31 tayatgtgyg ttktrccatc 20 <210> 32 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Alpha5385F primer <400> 32 ayaataattg ttgggtkaat gc 22 <210> 33 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha5546F primer <400> 33 aagggtgatg ctgarga 17 <210> 34 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha6842F primer <400> 34 tatgttaatg cwaatggtgg t 21 <210> 35 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha7517F primer <400> 35 gcktgytgta tgcgygc 17 <210> 36 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha8201F primer <400> 36 ggtggtraya atgtttattg yta 23 <210> 37 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha8432F primer <400> 37 tggtwtgtta aygatggamg tgt 23 <210> 38 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha8933F primer <400> 38 garagtgctg cwgcwgg 17 <210> 39 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Alpha8961F primer <400> 39 twgayatkcg ttcttatga 19 <210> 40 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha9077F primer <400> 40 taycgytgyg cttgttatgc 20 <210> 41 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha9203F primer <400> 41 atggcrcarc crtctgg 17 <210> 42 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha10476F primer <400> 42 tggcgytttk ctaraga 17 <210> 43 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha10814F primer <400> 43 aartayatgr twgctaatgg 20 <210> 44 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha11113F primer <400> 44 taarcayagt gattttggwc t 21 <210> 45 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha11189F primer <220> <221> modified_base <222> (3) <223> i <400> 45 tcntcwtttg ttrgtatgcc 20 <210> 46 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha11360F primer <400> 46 agratggctg aacaagctgc 20 <210> 47 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha11507F primer <400> 47 gcacgtratg gtgttgt 17 <210> 48 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha11624F primer <220> <221> modified_base <222> (9) <223> i <400> 48 taygckggng ttgtttggac 20 <210> 49 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha12179F primer <400> 49 gctgttarac awggygcaaa acc 23 <210> 50 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Alpha12255F primer <400> 50 ctggtarygg tcargctat 19 <210> 51 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha12532F primer <400> 51 tctagtgcmg ctcgactaga 20 <210> 52 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha12717F primer <400> 52 ggamcacgag cartccatgt a 21 <210> 53 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha13477F primer <400> 53 gtktttatwg atggtgtwcc 20 <210> 54 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Alpha13816F primer <400> 54 ggtgatgctg cwrttaaaga ttttga 26 <210> 55 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha13868F primer <400> 55 cttaataara gygctggktg gcc 23 <210> 56 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha14036F primer <220> <221> modified_base <222> (6) <223> i <400> 56 caraanggtg atgctgc 17 <210> 57 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha14220F primer <400> 57 cttaataara gygctgkttg gcc 23 <210> 58 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha14742F primer <400> 58 ggtacractt cwggtgaygc 20 <210> 59 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha15343F primer <400> 59 cckatgttgt gyactaartg 20 <210> 60 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha15532F primer <220> <221> modified_base <222> (12) <223> i <400> 60 gctggwaatr tntttggttt 20 <210> 61 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Alpha16114F primer <220> <221> modified_base <222> (15) <223> i <400> 61 acaatacagg gtccncctgg tagtgg 26 <210> 62 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha16561F primer <400> 62 cgwatgtgtg cwataggmcc 20 <210> 63 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha17266F primer <400> 63 cctggyagtc ayagtttgtt 20 <210> 64 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha17320F primer <400> 64 ggttggttrg gwatggatgt 20 <210> 65 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha17989F primer <220> <221> modified_base <222> (18) <223> i <400> 65 tttaaacayg cwttgggntg tga 23 <210> 66 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha18574F primer <400> 66 tttgawkktt ataatttgtg gca 23 <210> 67 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha18874F primer <400> 67 ttrtgggatt atgaagcwga 20 <210> 68 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha19333F primer <400> 68 tatggtgatg tktcwaaaac tac 23 <210> 69 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha19735F primer <400> 69 tgtaatttrt ataattaygg tgc 23 <210> 70 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha20047F primer <400> 70 tcwgatatgt aygatggyag 20 <210> 71 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha20829F01 primer <400> 71 tgyttatggt ctgtttctgg 20 <210> 72 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha20829F02 primer <400> 72 tgyttatggc ctgtacctgg 20 <210> 73 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha20829F03 primer <220> <221> modified_base <222> (7) <223> i <400> 73 tgyytcnggc ctgtwcctgg 20 <210> 74 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Alpha20846F01 primer <400> 74 ggttttgtyt attttaaygg tactgg 26 <210> 75 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Alpha20846F02 primer <400> 75 ggttttgtyt attttaaygc cactgg 26 <210> 76 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha22436F primer <400> 76 ctyaagcagt ayacttctgc ttg 23 <210> 77 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Alpha22457F primer <400> 77 tgtaaaacya twgaagaygc ctta 24 <210> 78 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Alpha22789F primer <400> 78 tgatgcwgar cgwatggc 18 <210> 79 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Alpha23909F primer <220> <221> modified_base <222> (6) <223> i <400> 79 tggccntggt gggtktgg 18 <210> 80 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha24066F primer <400> 80 tcaactaaac ttccttatta yga 23 <210> 81 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Alpha25053F primer <220> <221> modified_base <222> (9) <223> i <400> 81 gttggaatnt watyctaac 19 <210> 82 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha25287F01 primer <400> 82 gtttcagact tttccgwcgt g 21 <210> 83 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha25287F02 primer <400> 83 gtttcagact ttggcgccgt g 21 <210> 84 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Alpha25679F primer <220> <221> modified_base <222> (12) <223> i <400> 84 ttgcttcatt tnwtctaaac traac 25 <210> 85 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Alpha25980F primer <400> 85 ggtgtygtyt gggttgct 18 <210> 86 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Alpha27065F primer <400> 86 tagtcttaya cacaatggta rgcc 24 <210> 87 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Alpha27168F primer <400> 87 tggagtamty aaagatcgc 19 <210> 88 <211> 27317 <212> DNA <213> Human alphacoronavirus 229E|NC_002645:1 <400> 88 acttaagtac cttatctatc tacagataga aaagttgctt tttagacttt gtgtctactt 60 ttctcaacta aacgaaattt ttgctatggc cggcatcttt gatgctggag tcgtagtgta 120 attgaaattt catttgggtt gcaacagttt ggaagcaagt gctgtgtgtc ctagtctaag 180 ggtttcgtgt tccgtcacga gattccattc tacaaacgcc ttactcgagg ttccgtctcg 240 tgtttgtgtg gaagcaaagt tctgtctttg tggaaaccag taactgttcc taatggcctg 300 caactgtgtg acacttgccg tagcaagtga ttctgaaatt tctgcaaatg gctgttctac 360 tattgcgcaa gccgtccgcc gttatagcga ggccgctagc aatggtttta gggcatgccg 420 atttgtttca ttagatttgc aggattgcat cgttggcatt gcagacgata catatgttat 480 gggtctgcat ggcaatcaga cgttgttttg caacataatg aaattttctg accgtccttt 540 tatgcttcat gggtggttgg ttttttccaa ttcaaattac cttttggagg aatttgatgt 600 tgtcttcggt aagagaggtg gtggtaatgt gacatacact gaccagtatc tctgtggcgc 660 cgatggcaaa cctgttatga gtgaagattt atggcagttt gttgaccatt tcggtgagaa 720 cgaagaaatt atcatcaatg gtcatactta cgtttgtgct tggcttacta agcgtaagcc 780 cttagattac aaacgtcaga acaaccttgc cattgaagag attgaatatg tgcatggtga 840 tgctttgcat acactacgca atggttctgt tcttgaaatg gctaaggaag tgaagacatc 900 tagtaaagtt gtgttaagcg atgctcttga caaactttac aaagtctttg gttctcctgt 960 tatgacaaat ggttccaaca tcctagaggc ctttactaaa cctgtgttta ttagtgcatt 1020 agttcaatgt acttgtggta ccaagtcttg gtctgttggt gattggaccg gttttaaatc 1080 ctcttgttgc aacgtgatca gtaataaact gtgtgttgtt cccggtaatg ttaaacctgg 1140 tgatgctgtg attaccactc agcaagctgg tgctggtatt aagtattttt gtggcatgac 1200 tcttaagttt gttgcaaata ttgaaggtgt ctctgtttgg agagtgattg ctcttcagag 1260 tgtggattgc tttgttgctt cttccacttt tgtagaagag gaacatgtta atagaatgga 1320 tacattctgc ttcaatgtac gcaatagtgt tactgatgag tgtcgtctgg ccatgttggg 1380 tgctgaaatg actagtaatg tcagaagaca agttgcttca ggtgtcatag acattagtac 1440 cggttggttt gatgtttatg atgacatctt tgctgaaagc aaaccatggt ttgttcgcaa 1500 ggctgaagac atttttggcc cttgttggtc cgctcttgtt tctgcactta aacaacttaa 1560 agtcactaca ggtgaacttg tgagatttgt taagtctatt tgcaattcag ctgttgctgt 1620 cgtgggtggt actatacaaa ttctcgctag tgtgcctgag aagtttttga atgcgtttga 1680 cgtgtttgtc acagctattc aaactgtctt tgactgtgct gttgaaactt gtactattgc 1740 cggtaaagca tttgacaagg tttttgacta tgtcttgctt gataatgcgc ttgtaaaact 1800 tgtcaccaca aagcttaagg gtgttcgtga acgtggcctt aataaagtta agtatgcaac 1860 agttgttgtt ggttccactg aagaagttaa atcttcacgt gttgaacgta gcactgctgt 1920 acttacaatc gccaacaatt attccaaact ttttgatgaa gggtatactg ttgtaattgg 1980 cgatgtggcg tactttgtta gtgacggcta cttccgtctt atggccagtc caaatagtgt 2040 gttgactact gcagtctata aaccattgtt tgcttttaat gtgaatgtta tgggtactag 2100 acctgaaaaa tttccaacca ctgtgacttg tgaaaattta gagtctgttg ttttgtttgt 2160 taatgacaaa attactgaat tccaattgga ttacagtatt gatgtcattg ataatgaaat 2220 aattgtcaaa cctaatatca gcctatgtgt tccactttat gtgagagact atgttgacaa 2280 atgggatgat ttttgcagac aatatagtaa cgagtcttgg tttgaggatg attacagggc 2340 ttttatcagt gttttggaca tcactgatgc tgctgtgaaa gctgcagagt ctaaagcttt 2400 cgttgatact attgttccac cttgcccatc tattttgaaa gttatagatg gaggcaaaat 2460 atggaatggt gttattaaaa atgttaactc tgttagagac tggcttaagt ctttgaagtt 2520 aaatctcaca caacagggtt tgcttggaac atgtgcaaag cgttttaaac gttggcttgg 2580 cattttgcta gaggcctata atgcgttttt agacactgtg gtttctactg ttaaaattgg 2640 tggcttgacc tttaaaacat atgcttttga taaaccttac attgtgatac gtgatatcgt 2700 gtgtaaggtt gaaaataaaa cagaagcaga atggattgag ctttttccac ataatgacag 2760 gattaagtct tttagtactt tcgagagtgc ttacatgcca attgcagacc ctacacattt 2820 tgacattgaa gaagttgaac ttttagatgc agagtttgta gaaccaggct gtggtggtat 2880 tttggcagta atagatgagc acgtctttta taagaaggat ggtgtttatt atccatcaaa 2940 tggtactaac attctacctg ttgcatttac aaaagccgct ggtggtaaag tttcattttc 3000 tgatgacgtt gaagtaaaag acattgaacc tgtttacaga gtcaagcttt gctttgagtt 3060 tgaagatgaa aaacttgtag atgtttgtga aaaggcaatt ggcaagaaaa ttaaacatga 3120 aggtgactgg gatagctttt gtaagactat tcaatcagca ctttctgttg tttcttgcta 3180 tgtaaatcta cctacttatt acatttatga tgaagaaggc ggtaatgact tgagtttgcc 3240 cgttatgatt tctgaatggc ctctttctgt tcaacaagct caacaagaag ctactttacc 3300 tgatattgct gaggatgttg ttgaccaagt tgaagaagtc aatagcattt ttgacattga 3360 gacagtggat gttaaacatg atgtgagtcc ttttgaaatg ccatttgaag agttaaatgg 3420 tttaaagata ctcaaacaat tggataacaa ctgctgggtt aactcagtta tgttacaaat 3480 acaattaact ggtatacttg atggtgacta tgctatgcag ttttttaaaa tgggccgagt 3540 tgccaagatg attgaacgct gctacactgc tgagcaatgt atacgtggtg ctatgggtga 3600 tgttggtttg tgtatgtata gactgcttaa agacttacac actggtttta tggttatgga 3660 ttataaatgt agttgtacca gtggtaggct tgaagaatcg ggagctgttt tgttttgtac 3720 gcccactaag aaggcgtttc cttatggtac ttgtctaaat tgtaacgcac ctcgcatgtg 3780 tacaattagg cagttacaag gtaccataat atttgtgcaa caaaaaccag aacctgttaa 3840 tcctgtttct tttgttgtta aaccagtctg ctcatcaatt tttcgtggtg ctgtgtcttg 3900 tggtcattac cagactaaca tctattcaca aaatttgtgt gtggatggtt ttggtgttaa 3960 caagattcag ccctggacaa atgatgcact taatactatt tgtattaagg atgcagatta 4020 taatgcaaaa gttgaaatat ctgttacacc aattaaaaat acagttgata caacacctaa 4080 ggaagaattt gttgttaaag agaagttgaa cgccttcctc gttcatgaca atgtagcttt 4140 ctaccaaggt gatgttgata ctgttgttaa tggtgttgac tttgacttta ttgtaaatgc 4200 tgctaatgag aaccttgctc atggtggagg acttgccaaa gctttagatg tgtacactaa 4260 aggtaaactt caacgtttat ctaaagaaca cattggatta gcgggtaaag taaaagttgg 4320 tacaggagtt atggttgagt gtgatagcct tagaattttt aatgttgttg gtccacgcaa 4380 gggtaaacat gaacgtgatt tactcataaa agcttacaac actattaata atgaacaagg 4440 cacaccttta acaccaattt tgagctgtgg tatttttggt atcaaactcg aaacttcatt 4500 agaagttttg cttgatgttt gtaatacaaa agaagttaaa gtttttgttt atacagacac 4560 agaggtttgt aaggttaagg attttgtgtc tggtttagtg aatgttcaaa aagttgagca 4620 acctaaaata gaaccaaaac cagtgtccgt aattaaagtt gcacccaagc cttacagggt 4680 agatggtaaa tttagttact ttacagaaga cttgttgtgt gtcgctgatg acaaacccat 4740 tgttttgttt actgactcta tgcttacttt ggatgaccgt ggtttagctc tagacaatgc 4800 acttagtggt gtgcttagtg ctgctattaa ggattgtgtt gacataaata aagctatacc 4860 ttctggtaat cttattaagt ttgatatagg ttctgttgtt gtctacatgt gtgttgtgcc 4920 atccgaaaag gacaaacatt tagataataa tgttcaacga tgcacacgta agttgaatag 4980 acttatgtgt gatatagttt gtactatacc agctgactac atcttgccat tggtgttgtc 5040 tagtttgact tgtaatgttt cttttgtagg tgaacttaaa gctgctgaag ctaaagttat 5100 aactataaag gtgacagagg atggtgttaa tgttcatgat gtgaccgtga caacagacaa 5160 gtcatttgaa caacaagttg gtgttattgc tgataaggac aaagatcttt ctggtgcagt 5220 accaagtgat cttaacacat ctgaattgct tactaaagca atagatgttg attgggtcga 5280 attttatggc tttaaagatg ctgttacttt tgcaacagtt gatcatagtg cttttgccta 5340 tgaaagtgct gttgttaatg gtattagagt gttaaaaact agtgataata attgttgggt 5400 gaatgctgtt tgtattgcac tacagtattc gaaaccccat tttatttcac aaggtcttga 5460 tgctgcgtgg aataaatttg ttttaggcga tgttgaaatt tttgttgcat ttgtttacta 5520 tgttgcaaga ctaatgaaag gtgacaaggg tgatgctgaa gacactttga ctaagttgtc 5580 taagtatctt gctaatgaag ctcaagttca attagaacat tatagttctt gtgttgaatg 5640 tgatgctaaa tttaaaaact ctgttgcatc tatcaattct gctatagttt gtgctagtgt 5700 caaacgtgat ggtgtgcaag ttggttattg tgtccatggt attaagtact attcacgtgt 5760 tagaagtgtt agaggtagag ctattatagt cagtgtcgaa cagcttgaac cgtgtgctca 5820 gtctagactt ttgagtggtg ttgcttatac tgctttttct ggacctgttg acaaaggtca 5880 ttatactgtt tatgatactg caaagaaatc aatgtatgat ggtgatcgtt ttgttaaaca 5940 tgatctttct ctgctgtctg tcacatcagt tgttatggtt ggtggttatg ttgcacctgt 6000 taatacagtg aaacctaaac cagtcattaa tcaacttgat gaaaaggcac agaagttctt 6060 tgattttggt gattttttga ttcataattt tgttattttt ttcacatggt tattgagtat 6120 gtttactttg tgtaaaactg cagtaactac aggtgatgtt aaaataatgg ccaaagcacc 6180 acaaaggacg ggtgttgttt taaaacgtag tcttaaatat aacttaaaag cgtcagcagc 6240 tgttcttaaa tctaagtggt ggctgcttgc taagtttacg aaactactgt tactcatata 6300 tacattgtac tcagtagttt tgctttgtgt acgttttgga ccgtttaatt tttgtagtga 6360 gactgttaat ggttatgcta agtcaaactt tgtcaaggat gattactgtg atggttcatt 6420 gggctgcaag atgtgtcttt ttggttacca agagttaagt caatttagcc atttggatgt 6480 tgtgtggaag catataacag accctttgtt tagtaatatg caacctttca ttgtcatggt 6540 tttgctgctt atatttggtg acaattattt gagatgcttc ttgctgtatt ttgttgctca 6600 gatgataagc acagttggtg tttttctagg ttacaaggaa acaaattggt tcttgcactt 6660 tattccattt gatgttattt gtgatgaact gcttgtcact gttattgtta ttaaggttat 6720 ttcttttgtc agacatgtgc tttttggttg tgaaaaccca gattgcattg cgtgttctaa 6780 gagtgctaga cttaagagat tccctgttaa cacaattgtc aatggtgtgc aacgttcatt 6840 ttatgttaat gcaaatggtg gtagtaagtt ttgtaagaaa catagatttt tctgtgttga 6900 ttgtgactct tatggttatg gcagcacgtt tataacaccc gaagtttcta gagaacttgg 6960 taacattacc aaaacaaatg tgcaaccaac aggcccagcg tatgtcatga ttgacaaagt 7020 ggagtttgaa aatggttttt acagattgta ttcctgtgaa acattttggc gttacaactt 7080 tgatataact gaaagcaagt attcttgcaa agaggttttt aaaaattgta atgttttgga 7140 tgatttcatc gtgtttaaca ataatgggac caatgtaacg caggttaaaa atgctagtgt 7200 ttacttttca cagttgttgt gtaggcccat taaattagtt gacagtgaac ttttgtccac 7260 tttgtcagtt gattttaatg gtgtcttaca caaggcatac attgatgtac tacgtaatag 7320 ctttggtaaa gatcttaatg ctaatatgtc tttagccgag tgcaagagtg ctttaggcct 7380 gtctattagt gatcatgaat ttactagtgc tatttctaat gcacatcgtt gtgacgtgtt 7440 gttatctgat ttgtcattta acaactttgt cagttcgtat gctaaacctg aggaaaaatt 7500 atcagcttat gacttggcgt gttgtatgcg tgcaggtgct aaggttgtta atgccaatgt 7560 tctgacaaag gaccaaactc ctattgtttg gcatgcaaag gattttaaca gtctttctgc 7620 tgaaggtcgc aagtatattg taaaaactag caaagctaag ggtttgactt tcttgttgac 7680 aattaatgaa aaccaagctg tcacgcaaat acctgcaact agcattgttg ctaagcaagg 7740 tgctggtgat gctggccatt cattaacatg gctgtggcta ctgtgtggtc ttgtgtgttt 7800 gattcaattc tacttgtgct ttttcatgcc ctattttatg tacgatatcg tgagtagttt 7860 tgagggttat gattttaagt atatagaaaa tggtcagttg aagaattttg aagcgccact 7920 taaatgcgtc agaaacgttt ttgaaaactt tgaggactgg cattatgcta agtttggctt 7980 cacaccttta aacaagcaaa gctgtcctat tgtagttgga gtttctgaaa ttgttaatac 8040 tgtcgctggc attccatcta atgtgtatct tgttggtaaa actttaattt ttacactaca 8100 agctgctttt ggtaatgctg gtgtttgtta tgacattttt ggagtcacaa cacctgaaaa 8160 gtgcattttt acttctgctt gtactagatt agaaggtttg ggtggtgaca atgtttattg 8220 ttataacaca gcgcttatgg aaggttcttt gccttacagt tcaatacaag ctaatgcata 8280 ttataaatat gacaatggca attttattaa gttgccagaa gttattgcac aaggctttgg 8340 ttttagaaca gtgcgtacta ttgccaccaa atactgccgc gtaggtgaat gtgttgaatc 8400 caatgcaggt gtgtgttttg gctttgacaa gtggtttgtt aacgatggac gtgttgccaa 8460 tggttacgtt tgtggtactg gtttgtggaa ccttgtattt aacatacttt ccatgttttc 8520 atcttcattc tctgttgctg caatgtcagg tcaaatttta cttaattgtg cattaggtgc 8580 ttttgctatt ttttgttgtt ttcttgtgac aaagtttaga cgcatgtttg gtgacctttc 8640 tgtaggtgtt tgcactgttg ttgtggctgt tttgcttaac aatgtctctt acattgtaac 8700 tcagaattta gtaacaatga ttgcttatgc catattgtat ttctttgcta ctagaagctt 8760 acgctatgca tggatttggt gtgctgcata tttaattgcg tatatttctt ttgctccatg 8820 gtggttgtgt gcttggtact ttcttgctat gttgacaggt ttgttaccta gtttgctgaa 8880 gcttaaagtt tcgacaaatc ttttcgaagg tgacaaattt gtaggtacat ttgaaagtgc 8940 tgctgcagga acatttgtca ttgacatgcg ttcttatgag aaacttgcta atagcatctc 9000 tccagaaaag ttgaaaagtt atgctgctag ctataataga tataagtact atagtggtaa 9060 tgcaaatgaa gctgattact gttgcgcttg ttatgcctat ttagcaaaag caatgttgga 9120 cttttcgcgt gatcataatg acatcttgta cacacctccg actgtcagtt atggttctac 9180 attacaggct ggtttgcgca aaatggcaca accatctggc tttgtggaga aatgtgttgt 9240 ccgtgtctgc tatggaaaca ctgtgttgaa tgggttgtgg cttggtgata ttgtttattg 9300 cccacgtcat gttatcgcat ctaacacaac ttctgctata gattatgatc acgaatatag 9360 tattatgcgg ttgcataatt tttctataat atctggtaca gcatttcttg gtgttgtagg 9420 tgctactatg catggagtaa ctcttaaaat taaggtttca cagactaaca tgcacacacc 9480 tagacattct tttagaacac taaaatctgg tgaaggtttt aacatcttag catgctatga 9540 tggttgtgct caaggtgttt ttggtgtgaa catgagaact aattggacta tccgtggttc 9600 atttattaat ggtgcgtgtg gttcccctgg ctacaatctt aaaaatggcg aggtggaatt 9660 tgtttatatg catcaaattg aactcggaag tggtagccat gtaggttcta gctttgatgg 9720 tgttatgtat ggtggttttg aagaccaacc taatcttcaa gttgaatctg caaaccagat 9780 gttaacagtt aatgtggttg catttcttta tgctgctata ttgaatggtt gcacatggtg 9840 gcttaaaggt gaaaaattgt ttgtggagca ttataatgag tgggcacagg ctaatggttt 9900 cacagctatg aatggtgaag acgctttttc cattcttgct gctaaaactg gtgtctgtgt 9960 ggaaagatta cttcatgcta ttcaagtttt gaataatggc tttggtggta aacaaatttt 10020 gggttattct agtctcaatg atgagttcag tattaatgaa gttgtcaaac aaatgtttgg 10080 tgttaacctg caaagtggta aaaccactag tatgtttaaa tccataagct tatttgctgg 10140 cttctttgtc atgttctggg ctgaattatt tgtttatacc accactattt gggttaaccc 10200 tggttttctt actccgttta tgattttgct tgttgctttg tcactctgtc ttacatttgt 10260 tgttaaacat aaggttttgt ttttgcaagt gtttttgttg ccttcaatta ttgtggctgc 10320 tattcaaaac tgtgcttggg actaccatgt tacaaaggtg ttggcagaga agtttgatta 10380 taatgtttct gttatgcaaa tggacatcca gggttttgtt aacattttta tttgtctttt 10440 tgttgcactg ttgcatactt ggcgctttgc taaagagcgt tgtacacatt ggtgcactta 10500 tttgttctca ctcattgctg ttttatacac tgcattgtat agttatgact acgttagttt 10560 gctggttatg ctactttgtg caatttctaa tgaatggtat attggtgcta ttatttttag 10620 aatttgtcgt tttggtgttg catttttacc agtggaatac gtgtcttact ttgatggtgt 10680 taaaactgtg ctgttgtttt acatgttgtt aggctttgtt agctgtatgt actatggttt 10740 gttgtactgg attaacaggt tctgtaagtg cacattaggt gtttatgatt tctgtgttag 10800 tccagccgaa tttaagtata tggttgctaa tggtttgaat gcaccaaatg gcccttttga 10860 tgcgctcttt ctgtctttta aactaatggg tattggcggt cctagaacca ttaaagtttc 10920 tactgtacag tctaaattga ctgatcttaa gtgcacaaac gtcgttctaa tgggcatttt 10980 gtctaacatg aacatagctt ctaattcaaa ggagtgggca tattgtgttg aaatgcacaa 11040 taaaataaac ttgtgtgacg accctgaaac tgctcaagag ttattgctgg cgttgttggc 11100 ctttttcttg tctaagcata gtgattttgg tcttggtgat cttgtcgatt cttattttga 11160 gaacgactcc attttgcaaa gtgttgcatc ttcttttgtt ggtatgccat cttttgttgc 11220 atatgaaaca gcaagacaag agtatgaaaa tgctgttgca aatggttcct caccacaaat 11280 aatcaaacaa ttgaagaagg ctatgaatgt tgcaaaagct gagtttgaca gggaatcatc 11340 tgttcaaaag aaaattaaca gaatggctga acaagctgct gcagctatgt acaaagaagc 11400 acgtgctgtt aatagaaaat caaaagttgt tagtgccatg catagtttac tctttggcat 11460 gctccgacgt ttggacatgt ctagtgttga cactatcctt aatatggcac gtaatggtgt 11520 tgtccctctt tccgttatcc ctgctacttc tgcagccagg ctcgtcgtcg tagtaccaga 11580 tcatgattca tttgtgaaaa tgatggtaga tggttttgtg cactacgctg gtgttgtttg 11640 gacattacag gaagttaagg ataatgatgg taagaatgtg catcttaaag atgttacaaa 11700 ggaaaaccag gaaatacttg tttggcctct gattttgact tgtgaacgtg tcgttaaatt 11760 gcagaacaat gaaataatgc cgggcaagat gaaggtcaag gccaccaaag gtgaaggtga 11820 tggaggcatt actagtgaag gtaatgctct atacaacaat gaaggtggac gtgcattcat 11880 gtatgcatat gtgactacga agcctggcat gaagtatgtt aaatgggaac atgactctgg 11940 tgtggttaca gttgaattgg aaccaccttg cagatttgtt atagacacac ctactggacc 12000 ccaaattaag tatctttatt ttgttaagaa tcttaacaat ttaaggagag gtgctgtttt 12060 gggttacatt ggtgccactg tgagattgca agctggcaaa cagactgagt ttgtttcaaa 12120 ctcccattta ttaacacatt gttcttttgc tgttgaccca gctgcagcct atcttgatgc 12180 tgttaaacaa ggcgcaaaac ctgttggcaa ttgtgtaaag atgttgacta atggttctgg 12240 tagcggtcag gctattactt gtaccattga ttccaacact acgcaggaca catatggtgg 12300 cgcgtctgtt tgtatttatt gcagagcaca tgttgcacat ccaaccatgg acggtttttg 12360 tcagtacaaa ggcaagtggg tacaagtgcc tataggtaca aatgacccta taagattttg 12420 tcttgaaaat actgtttgta aagtttgtgg ttgttggctt aatcatggct gtacatgtga 12480 ccggactgct atccaaagtt ttgataacag ttatttaaac gagtccgggg ctctagtgcc 12540 gctcgactag agccctgtaa tggtacagac atagattact gtgtccgtgc atttgacgtt 12600 tacaataaag atgcgtcttt tatcgggaaa aatctgaagt ccaattgtgt gcgcttcaag 12660 aatgtagata aggatgacgc gttctatatt gttaaacgtt gcattaagtc agttatggac 12720 cacgagcagt ccatgtataa cttacttaaa ggctgtaatg ctgttgctaa gcatgatttc 12780 tttacttggc atgagggcag aaccatttat ggtaatgtta gtagacagga tcttactaaa 12840 tacaccatga tggatttgtg cttcgctctg cgtaactttg atgaaaaaga ctgtgaagtt 12900 tttaaggaga tattggttct tactggttgt tgtagtactg attactttga aatgaagaat 12960 tggtttgacc ccatagaaaa tgaggacata caccgtgtgt atgctgcttt aggtaaggta 13020 gttgcaaatg caatgcttaa gtgtgttgct ttttgcgacg aaatggtgct caaaggagtt 13080 gttggtgttt tgaccttaga caaccaagat cttaatggga atttctatga cttcggtgac 13140 tttgtattgt gtcctcctgg aatgggaata ccctactgca cgtcatacta ttcttatatg 13200 atgcctgtta tgggtatgac taattgttta gctagtgagt gctttatgaa aagtgacatc 13260 tttggtcaag acttcaaaac ttttgatttg ttgaaatatg atttcacaga acataaggag 13320 gttttgttta acaagtactt taagtattgg ggacaggatt atcatcctga ttgtgttgat 13380 tgccatgacg agatgtgtat tttgcattgt tcaaatttta acacactctt cgcaaccaca 13440 attccaaaca cggcttttgg acctctatgc agaaaagtgt ttattgatgg tgtacccgta 13500 gttgctactg ctggttacca ctttaaacaa ttaggacttg tgtggaacaa agatgttaac 13560 actcattcta ccagacttac tattactgaa ctcttacagt ttgtgacaga tccaacgctt 13620 atagttgcgt catcgcctgc cttggtggat aaacgcactg tttgtttttc tgtcgctgct 13680 ttgagtacag gattaacatc ccaaacagta aaacctggcc attttaataa ggagttttat 13740 gacttcttac gttctcaggg gtttttcgat gagggttcag aattaacatt gaagcatttc 13800 ttttttacac aaaagggtga tgctgcaatt aaagattttg attattatcg ttacaacaga 13860 cctactatgc tggatattgg acaagctcgc gtagcatatc aagtggcagc tcgctatttt 13920 gactgttacg agggtggctg tattacatct agagaggttg ttgttacaaa ccttaataaa 13980 agcgctggtt ggccccttaa taagtttggt aaagctggtt tatattatga gtctattagt 14040 tatgaggaac aagatgctat tttttcatta acaaagcgta atattctccc tactatgact 14100 cagttaaatc ttaaatacgc catatctggt aaggaacgcg cacgtacagt gggtggcgtc 14160 tctttattag ctactatgac tacaagacag tttcatcaga aatgtctgaa atccatagta 14220 gctaccagaa atgccaccgt tgttatcggc actaccaagt tttatggcgg gtgggataat 14280 atgttaaaga acctgatggc cgatgttgat gatcctaaat tgatgggatg ggactatcct 14340 aagtgtgata gagctatgcc ctcaatgatt cgtatgttgt cggctatgat cttaggttct 14400 aagcatgtca catgttgtac ggctagtgat aaattttata gacttagtaa tgagcttgct 14460 caagttttga ccgaggttgt ttattcaaat ggtgggtttt attttaaacc tggtggtaca 14520 acttctggtg atgcaactac agcctatgcc aattctgtct ttaatatatt tcaggctgta 14580 agttctaaca ttaattgcgt tttgagcgtt aactcgtcaa attgcaataa ttttaatgtt 14640 aagaagttac agagacaact ttatgataat tgctatagaa atagtaatgt tgatgaatct 14700 tttgtggatg acttttatgg ttatttgcaa aagcattttt ctatgatgat tctttctgat 14760 gatagtgttg tgtgctataa taaaacttat gctggacttg gttacattgc tgatattagt 14820 gcttttaaag ccactttgta ttatcagaat ggtgtgttta tgagtacagc taagtgttgg 14880 actgaggaag atctttctat aggacctcat gaattttgct cacagcacac tatgcagatt 14940 gtagatgaaa atggtaagta ttatctacca tatccagatc ctagccgtat tatttctgct 15000 ggtgtttttg tggatgacat cactaagact gatgctgtca ttcttttgga acgctatgtt 15060 tctctggcta tagatgccta cccattgtct aagcatccta aacctgagta caggaaggtg 15120 ttttacgcat tgttagactg ggtcaaacat ctcaacaaga ctcttaacga aggtgttttg 15180 gagtcttttt ctgttacact tttagatgaa catgagtcta agttttggga tgaaagcttt 15240 tatgctagta tgtatgagaa gtctacagta ttacaagctg ctggtctttg tgtagtatgt 15300 ggttctcaaa cagttctaag atgcggtgat tgtttacgca gaccgatgtt gtgcactaag 15360 tgcgcctatg atcatgtgtt tggcactgat cataagttca ttttagctat tacaccatat 15420 gtgtgtaaca catctggctg caatgtaaat gacgttacaa aactgtatct tggaggtttg 15480 aattattact gtgtagacca caaaccacat ctttcattcc cactgtgttc agttggtaat 15540 gtctttggtt tgtacaaaag ttctgctttg ggttccatgg acattgatgt ctttaacaaa 15600 ctttctacct ctgattggtc tgacattcgc gactacaagc ttgctaatga tgcaaaagag 15660 tcactaaggt tgtttgcagc tgaaacggtc aaggctaaag aggaaagtgt taagtcatca 15720 tacgcttatg ctaccctaaa ggagattgta ggtcctaagg aacttttgct cttatgggaa 15780 agtggaaaag ccaaaccacc gttaaaccgt aattctgttt ttacatgctt ccaaattaca 15840 aaagactcca agtttcaagt tggtgagttt gtgtttgaga aagtagatta cggttctgat 15900 acggttactt acaaatccac tgctactact aagttagtac caggtatgtt gtttattttg 15960 acttctcata atgttgctcc acttagagcg ccaacaatgg caaaccagga gaaatattct 16020 accatttaca agttgcaccc atcatttaat gttagtgatg cttatgcaaa tcttgtacct 16080 tattaccaac ttattggcaa acagcgtatc accacaatac agggtcctcc tggtagtgga 16140 aaatcgcatt gttctattgg tattggtgtg tattaccctg gagcgaggat cgtgttcacc 16200 gcttgttctc acgctgctgt tgattcgctc tgtgcaaaag ctgtcacagc ctatagtgtt 16260 gataagtgta cacgtattat tcctgcacgt gccagagttg agtgttatag tggttttaaa 16320 cctaacaata atagtgcaca atacgtgttt agtactgtta atgcgttacc tgaagttaat 16380 gcagacattg ttgtcgtgga tgaggtgtct atgtgcacta actatgactt gtctgtgatt 16440 aaccagcgta tatcatataa acacattgta tatgttggtg atcctcaaca gcttccagct 16500 cctagagttc ttatctctaa aggtgttatg gaaccaattg actataatgt tgtgacacaa 16560 cgtatgtgtg ctataggacc cgatgtcttt ttacacaagt gttacagatg tcctgctgaa 16620 atagttaaca ctgtttcaga gcttgtttat gaaaacaagt ttgtacctgt caaagaagct 16680 agtaagcagt gcttcaaaat ctttgaacgc ggtagtgttc aggtagacaa tggctccagt 16740 ataaataggc gtcaacttga tgttgttaag cgatttatac ataaaaactc cacatggagc 16800 aaggctgtgt ttatctcacc ttacaatagt caaaattatg tagctgccag gcttttaggc 16860 ttacaaactc agacagtgga ttctgctcaa ggtagtgaat atgactatgt tatattcgca 16920 cagacatcag atactgctca tgcctgtaat gccaatcgtt ttaacgttgc cattactaga 16980 gcaaagaaag gtattttctg tattatgtct gacagaactt tgtttgatgc acttaagttc 17040 tttgaaatca ctatgacaga tttacagtct gaaagtagtt gtggtttgtt taaggattgt 17100 gcacgtaacc ctattgattt accaccaagt catgccacta cttatttgtc attgtctgat 17160 agatttaaga ctagtggtga cttggctgtt caaataggta acaacaatgt ttgtacctat 17220 gaacatgtga tttcatatat gggtttcagg tttgatgtta gcatgcctgg tagtcatagt 17280 ttgttctgta ctagagactt tgccatgcgt catgtcagag gttggttagg aatggatgtg 17340 gaaggtgcac atgtcacagg tgacaatgtt ggcactaatg tacctctaca agttggtttt 17400 tccaatggtg ttgattttgt agctcaacct gaaggttgtg ttctaacaaa cactggcagt 17460 gttgtaaaac ctgttcgtgc tcgtgcacca cctggagaac aatttactca cattgtacct 17520 ctgttacgca agggacaacc ttggagtgtg ttgagaaaac gtattgttca aatgatagca 17580 gattttcttg ctggctcatc tgatgtactg gtgtttgtac tttgggctgg cggtttagag 17640 ttgaccacta tgcgttattt tgttaagatt ggagctgtta aacattgcca atgtggtact 17700 gttgcaacat gctacaattc tgttagtaat gactattgtt gctttaaaca tgcattgggc 17760 tgtgactatg tttataatcc atatgtcata gatattcaac aatggggtta tgttggttca 17820 ctctccacta atcaccatgc aatttgtaat gttcatagaa atgagcatgt tgcttctggt 17880 gatgctatta tgactagatg tttggctgtg tatgactgct ttgttaagaa tgtggattgg 17940 tcaattacct accctatgat agctaatgaa aatgccataa acaagggcgg tcgcactgtg 18000 cagagtcata ttatgcgtgc tgctattaaa ttgtacaacc ctaaagcaat ccatgacatt 18060 ggtaatccta agggtattcg ttgtgctgta actgatgcca agtggtattg ttatgacaag 18120 aaccctatta attctaatgt gaaaacattg gagtatgatt acatgacaca tggccaaatg 18180 gatggcttgt gtttgttttg gaattgtaat gtggatatgt accctgaatt ctcaattgtt 18240 tgcaggtttg acacacgtac acgatctaca ttgaaccttg aaggtgtaaa tggtgggtca 18300 ttgtatgtca ataatcatgc atttcacact cctgcttatg ataaacgtgc tatggctaaa 18360 ttgaaaccag caccgttttt ctactatgac gacggttcat gtgaggttgt tcacgatcaa 18420 gttaactatg ttcctttgag agccactaat tgcattacca agtgtaatat tggtggtgct 18480 gtatgttcta agcacgctaa tctctataga gcatatgttg agtcatataa catttttact 18540 caagctggtt ttaatatttg ggttcctacc acgtttgatt gttataattt gtggcagaca 18600 ttcacagagg tcaatttaca aggtttagag aacattgctt ttaacgttgt taataaaggt 18660 tcatttgttg gtgctgatgg tgaattacca gtagccatta gtggtgataa agtgttcgta 18720 cgtgatggta acactgataa tttagtcttt gttaacaaaa catcactgcc tacaaacata 18780 gcatttgaac tttttgctaa gaggaaggtt ggtttaacac cacctctcag tattctcaaa 18840 aaccttggtg ttgtcgccac atataagttt gtcttgtggg attatgaagc tgagcgtccc 18900 ttgacaagct ttactaagtc tgtttgtggt tatacagact ttgcagagga tgtttgtact 18960 tgttacgata atagtataca aggttcatac gaacgtttta ctctgtcaac taatgctgtg 19020 ttattctctg ctactgctgt gaaaacaggt ggtaagagtt tgccggctat taaattgaat 19080 tttggaatgc ttaatggtaa tgcaattgct actgtcaaat cagaagatgg taacataaaa 19140 aatattaact ggtttgttta cgtacgcaaa gatggcaaac ctgttgatca ttatgatggt 19200 ttttataccc aaggtcgtaa tttacaagac tttttgcctc gcagcacaat ggaagaagac 19260 tttttgaaca tggatatagg cgtgtttatt caaaagtatg gtctagagga tttcaacttc 19320 gagcacgttg tgtatggtga tgtttcaaaa actactctag gcggtttaca cttgttgatt 19380 tcacaagtac gtctgagtaa aatgggcatc ttaaaggcag aggagtttgt ggcagcatct 19440 gacataacac tcaaatgttg tactgtgact tatcttaatg atcctagttc taagactgtt 19500 tgtacttaca tggatttgtt gttggatgat tttgtttctg tattgaagtc tttggatttg 19560 actgttgtat ccaaggttca tgaggtcata attgacaaca aaccatggag atggatgcta 19620 tggtgtaaag ataatgccgt tgctacattc tatcctcagt tgcagagtgc agaatggaaa 19680 tgcgggtatt ctatgcctgg tatttataag acacaacgta tgtgcttaga accatgtaat 19740 ttgtataatt atggtgcagg tttgaagttg cccagtggca ttatgttcaa tgttgttaaa 19800 tacactcaat tgtgtcaata ttttaacagt accacgttat gtgttcctca taatatgaga 19860 gtgttacact tgggtgctgg ctctgattat ggtgttgcac caggaactgc tgttcttaaa 19920 aggtggttgc cgcacgacgc aattgttgtt gacaacgatg ttgttgacta tgtgagtgac 19980 gctgatttta gtgttactgg tgattgtgca accgtttatt tggaagacaa gtttgacttg 20040 ttaatctctg atatgtacga tggcaggaca aaggcaattg atggtgaaaa tgtttcgaaa 20100 gaaggatttt tcacttacat ctatggtttc atttgtgaaa aacttgccat cggaggttcg 20160 attgctatta aagtaacaga gtatagctgg aataagaaat tgtatgaact tgtacaaaga 20220 ttttcttttt ggactatgtt ttgcacttct gttaatacgt catcatcaga agcctttgtt 20280 gtcggaatta actatcttgg tgatttcgca caaggacctt ttatagatgg taacataata 20340 cacgcaaatt atgtattttg gcgtaactcc actgttatga gtttgtccta caactctgtt 20400 ttagacctga gtaaatttaa ttgcaaacac aaagcgactg ttgttgtgca attaaaggat 20460 agtgatatta atgaaatggt gcttagtctt gttaggagtg gtaagttgct tgtaaggggt 20520 aatggcaagt gtttgagttt tagtaatcat ttagtctcaa ctaaataaaa tgtttgtttt 20580 gcttgttgca tatgccttgt tgcatattgc tggttgtcaa actacaaatg ggctgaacac 20640 tagttactct gtttgcaacg gctgtgttgg ttattcagaa aatgtatttg ctgttgagag 20700 tggtggttat ataccctccg actttgcatt caataattgg ttccttctaa ctaatacctc 20760 atctgttgta gatggtgttg tgaggagttt tcagcctttg ttgcttaatt gcttatggtc 20820 tgtttctggc ttgcggttta ctactggttt tgtctatttt aatggtactg ggagaggtga 20880 ttgtaaaggt ttttcctcag atgttttgtc tgatgtcata cgttacaacc tcaattttga 20940 agaaaacctt agacgtggaa ccattttgtt taaaacatct tatggtgttg ttgtgtttta 21000 ttgtaccaac aacactttag tttcaggtga tgctcacata ccatttggta cagttttggg 21060 caatttttat tgctttgtaa atactactat tggcaatgaa actacgtctg cttttgtggg 21120 tgcactacct aagacagttc gtgagtttgt tatttcacgc acaggacatt tttatattaa 21180 tggctatcgc tatttcactt taggtaatgt agaagccgtt aatttcaatg tcactactgc 21240 agaaaccact gatttttgca ctgttgcgtt agcttcttat gctgacgttt tggttaatgt 21300 gtcacaaacc tctattgcta atataattta ttgcaactct gttattaaca gactgagatg 21360 tgaccagttg tcctttgatg taccagatgg tttttattct acaagcccta ttcaatccgt 21420 tgagctacct gtgtctattg tgtcgctacc tgtttatcat aaacatacgt ttattgtgtt 21480 gtacgttgac ttcaaacctc agagtggcgg tggcaagtgc tttaactgtt atcctgctgg 21540 tgttaatatt acactggcca attttaatga aactaaaggg cctttgtgtg ttgacacatc 21600 acacttcact accaaatacg ttgctgttta tgccaatgtt ggtaggtgga gtgctagtat 21660 taacacggga aattgccctt tttcttttgg caaagttaat aactttgtta aatttggcag 21720 tgtatgtttt tcgctaaagg atatacccgg tggttgcgca atgcctatag tggctaattg 21780 ggcttatagt aagtactata ctataggctc attgtatgtt tcttggagtg atggtgatgg 21840 aattactggc gtcccacaac ctgttgaggg tgttagttcc tttatgaatg ttacattgga 21900 caaatgtact aaatataata tttatgatgt atctggtgtg ggtgttattc gcgttagcaa 21960 tgacaccttt cttaatggaa ttacgtacac atcaacttca ggtaaccttc tgggttttaa 22020 agatgttact aagggcacca tctactctat cactccttgt aacccaccag atcagcttgt 22080 tgtttatcag caagctgttg ttggtgctat gttgtctgaa aattttacta gttacggctt 22140 ttctaatgtt gtagaactgc cgaaattttt ctatgcgtcc aatggcactt ataattgcac 22200 agacgctgtt ttaacttatt ctagttttgg cgtttgtgca gatggttcta taattgctgt 22260 tcaaccacgt aatgtttcat atgatagtgt ttcagctatc gtcacagcta atttgtctat 22320 accttccaat tggaccactt cggtccaggt tgagtattta caaattacaa gtacacctat 22380 cgtagttgat tgctccactt atgtttgcaa tggtaatgtg cgctgtgttg aattgcttaa 22440 gcagtatact tctgcttgta aaaccataga agacgcctta agaaatagcg ccaggctgga 22500 gtctgcagat gttagtgaga tgctcacttt tgacaagaaa gcgtttacac ttgctaatgt 22560 tagtagtttt ggtgactaca accttagcag cgtcatacct agcttgccca caagtggtag 22620 tagagtggct ggtcgcagtg ccatagaaga catacttttt agcaaacttg ttacttctgg 22680 acttggcact gtggacgcag actacaaaaa ttgcactaag ggtctttcca ttgctgactt 22740 ggcttgtgct caatattata atggcattat ggttttgcct ggcgtcgctg atgctgaacg 22800 aatggccatg tatacaggtt ctttaattgg tggaattgct ttaggaggtc taacatcagc 22860 cgtttcaata ccattttcat tagcaattca ggcacgttta aattatgttg cattgcagac 22920 tgatgtttta caagaaaatc agaaaattct tgctgcatct tttaacaaag caatgaccaa 22980 catagtagat gcctttactg gtgttaatga tgctattaca caaacttcac aagccctaca 23040 aacagttgct actgcactta acaagatcca ggatgttgtt aatcaacaag gcaacttatt 23100 gaaccattta acttctcagt tgaggcagaa ttttcaagct atctctagct ctattcaggc 23160 tatctatgac agacttgaca ctattcaggc tgatcaacaa gtagataggc tgattactgg 23220 tagattggct gctttgaatg tattcgtttc tcatacattg actaagtaca ctgaagttcg 23280 tgcttccaga cagcttgcac aacaaaaagt gaatgagtgt gtcaaatccc agtctaagcg 23340 ttatggcttc tgtggaaatg gcactcacat tttctcaatt gttaatgctg ctcctgaggg 23400 gcttgttttt ctccacactg tcttgttgcc gacacaatat aaggatgttg aagcgtggtc 23460 tgggttgtgc gttgatggta caaacggtta tgtgttgcga caacctaatc ttgctcttta 23520 caaagaaggc aattattata gaatcacatc tcgcataatg tttgaaccac gtattcctac 23580 catggcagat tttgttcaaa ttgaaaattg caatgtcaca tttgttaaca tttctcgctc 23640 tgagttgcaa accattgtgc cagagtatat tgatgttaat aagacgctgc aagaattaag 23700 ttacaaattg ccaaattaca ctgttccaga cctagttgtc gaacagtaca accagactat 23760 tttgaatttg accagtgaaa ttagcaccct tgaaaataaa tctgcggagc ttaattacac 23820 tgttcaaaaa ttgcaaactc tgattgacaa cataaatagc acattagtcg acttaaagtg 23880 gctcaaccgg gttgagactt acatcaagtg gccgtggtgg gtgtggttgt gcatttcagt 23940 cgtgctcatc tttgtggtga gtatgttgct attatgttgt tgttctactg gttgctgtgg 24000 cttctttagt tgttttgcat cttctattag aggttgttgt gaatcaacta aacttcctta 24060 ttacgacgtt gaaaagatcc acatacagta atggctctag gtttgttcac attgcaactt 24120 gtgtctgctg ttaatcaatc gcttagcaat gcgaaagtta gtgctgaagt ttcacgacag 24180 gttatccaag acgtgaaaga tggcactgtt accttcaact tgctagcgta tacactaatg 24240 agcctctttg ttgtgtattt tgctttattt aaagcaagat cacaccgtgg cagagctgct 24300 cttatagtgt ttaaaattct aatccttttc gtttatgtgc cattgctgta ttggtctcaa 24360 gcatatattg acgcaacttt gattgctgta attttgcttg gaagattttt ccatacagct 24420 tggcactgct ggctctacaa gacatgggat ttcattgtct tcaatgtaac cacactttgc 24480 tatgcaaggt aagtgttggt ttcttgaaaa taaggctctg aaaccattcg tttgttttta 24540 cggaggggat caattccttt acataggcga cagaattgtt tcttatctct caactaacga 24600 cttgtacgtt gctcttagag gacgtattga taaagacctc agcctttcta gaaaggttga 24660 gttatataac agtgaatgtg tatacttgtt ttgtgaacac ccagctgttg gaatagtcaa 24720 cacagatttc aaattagaaa tccactaaga tgttccttaa gctagtggat gatcatgctt 24780 tggttgttaa tgtactactc tggtgtgtgg tgcttatagt gatactacta gtgtgtatta 24840 caataattaa actaactaag ctttgtttca cttgccatat gttttgtaat agaacagttt 24900 atggccccat taaaaatgtg taccacattt accaatcata tatgcacata gaccctttcc 24960 ctaaacgagt tattgatttc taaactaaac gacaatgtca aatgacaatt gtacgggtga 25020 cattgtcacc catttgaaga agtggaattt tggttggaat gttattctaa ccatattcat 25080 tgttattctt cagtttggac actataaata ctccagattg ttttatggtt tgaagatgct 25140 tgtactgtgg cttctttggc cactcgtact tgctttgtca atctttgaca cctgggctaa 25200 ttgggattct aattgggcct ttgttgcatt tagctttctt atggccgtat caacactcgt 25260 tatgtgggtg atgtacttcg caaacagttt cagacttttc cgacgtgctc gaactttttg 25320 ggcatggaat cctgaggtca atgcaatcac tgtcacaacc gtgttgggac agacatacta 25380 tcaacccatt caacaagctc caacaggcat tactgtgacc ttgctgagcg gcgtgcttta 25440 cgttgacgga catagattgg cttcaggtgt tcaggttcat aacctacctg aatacatgac 25500 agttgccgtg ccgagcacta ctataattta tagtagagtc ggaaggtccg taaattcaca 25560 aaatagcaca ggctgggttt tctacgtacg agtaaaacac ggtgattttt ctgcagtgag 25620 ctctcccatg agcaacatga cagaaaacga aagattgctt catttttcct aaactgaacg 25680 aaaagatggc tacagtcaaa tgggctgatg catctgaacc acaacgtggt cgtcagggta 25740 gaatacctta ttctctttat agccctttgc ttgttgatag tgaacaacct tggaaggtga 25800 tacctcgtaa tttggtaccc atcaacaaga aagacaaaaa taagcttata ggctattgga 25860 atgttcaaaa acgtttcaga actagaaagg gcaaacgggt ggatttgtca cccaagctgc 25920 atttttatta tcttggcaca ggaccccata aagatgcaaa atttagagag cgtgttgaag 25980 gtgtcgtctg ggttgctgtt gatggtgcta aaactgaacc tacaggttac ggtgttaggc 26040 gcaagaattc agaaccagag ataccacact tcaatcaaaa gctcccaaat ggtgttactg 26100 ttgttgaaga acctgactcc cgtgctcctt cccggtctca gtcgaggtcg cagagtcgcg 26160 gtcgtggtga atccaaacct caatctcgga atccttcaag tgacagaaac cataacagtc 26220 aggatgacat catgaaggca gttgctgcgg ctcttaaatc tttaggtttt gacaagcctc 26280 aggaaaaaga taaaaagtca gcgaaaacgg gtactcctaa gccttctcgt aatcagagtc 26340 ctgcttcttc tcaaacttct gccaagagtc ttgctcgttc tcagagttct gaaacaaaag 26400 aacaaaagca tgaaatgcaa aagccacggt ggaaaagaca gcctaatgat gatgtgacat 26460 ctaatgtcac acaatgtttt ggccccagag accttgacca caactttgga agtgcaggtg 26520 ttgtggccaa tggtgttaaa gctaaaggct atccacaatt tgctgagctt gtgccgtcaa 26580 cagctgctat gctgtttgat agtcacattg tttccaaaga gtcaggcaac actgtggtct 26640 tgactttcac tactagagtg actgtgccca aagaccatcc acacttgggt aagtttcttg 26700 aggagttaaa tgcattcact agagaaatgc aacaacatcc tcttcttaac cctagtgcac 26760 tagaattcaa cccatctcaa acttcacctg caactgctga accagtgcgt gatgaagttt 26820 ctattgaaac tgacataatt gatgaagtaa actaaacatg ccactgtgtt gtttgaaatt 26880 caggctttag ttggaatttt gcttttgttc ttgcttttat tatctttctt ttgcctgttt 26940 ttagagagat ttggcgcctt ggtgccgtag atgaatacat tgcttttctc tgatctatgt 27000 atgatggtac gatcagagct gcttttaatt aacatgatcc cttgctttgg cttgacaagg 27060 atctagtctt atacacaatg gtaagccagt ggtagtaaag gtataagaaa tttgctacta 27120 tgttactgaa cctaggtgaa cgctagtata actcattaca aatgtgctgg agtaatcaaa 27180 gatcgcattg acgagccaac aatggaagag ccagtcattt gtcttgagac ctatctagtt 27240 agtaactgct aatggaacgg tttcgatatg gatacacaaa aaaaaaaaaa aaaaaaaaaa 27300 aaaaaaaaaa aaaaaaa 27317 <110> KRIBB <120> Human alphacoronavirus universal primer sets for whole genome          amplification method and diagnosis kit <130> M18-5701 <160> 88 <170> KoPatentIn 3.0 <210> 1 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> 43 Forward primer <400> 1 tagactttgt gtctactyyt ctc 23 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 5385 Reverse primer <400> 2 gcattaccca acaattattt 20 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 4358 Forward primer <220> <221> modified_base <222> (18) <223> i <400> 3 tttaatgttg twggkccncg 20 <210> 4 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> 9077 Reverse primer <400> 4 gcataacaag ccacgta 17 <210> 5 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> 8201 Forward primer <400> 5 ggtggtraya atgtttattg yta 23 <210> 6 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> 11113 Reverse primer <400> 6 agccaaaatc acttgtta 18 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 9077 Forward primer <400> 7 taycgytgyg cttgttatgc 20 <210> 8 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> 13477 Reverse primer <400> 8 ggacaccatc ataaaac 17 <210> 9 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> 12255 Forward primer <400> 9 ctggtarygg tcargctat 19 <210> 10 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> 15343 Reverse primer <400> 10 cattagtcac aacatgg 17 <210> 11 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 13477 Forward primer <400> 11 gtktttatwg atggtgtwcc 20 <210> 12 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> 17266 Reverse primer <400> 12 aacaaacttg actccagg 18 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 15343 Forward primer <400> 13 cckatgttgt gyactaartg 20 <210> 14 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> 20047 Reverse primer <400> 14 ctccatctac atatcga 17 <210> 15 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 17266 Forward primer <400> 15 cctggyagtc ayagtttgtt 20 <210> 16 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> 22457 Reverse primer <400> 16 taaggctctt catgttttac a 21 <210> 17 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> 19333 Forward primer <400> 17 tatggtgatg tktcwaaaac tac 23 <210> 18 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> 24066 Reverse primer <400> 18 tctaataagg aagtttagtt ga 22 <210> 19 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> 22457 Forward primer <400> 19 tgtaaaacya twgaagaygc ctta 24 <210> 20 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> polyT Reverse primer <400> 20 tttttttttt tttgtgtatc catatc 26 <210> 21 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Alpha13F primer <400> 21 ttwtctatct ayagatagar aa 22 <210> 22 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Alpha41F primer <400> 22 tttagacttt gtgtctacty ytctc 25 <210> 23 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha43F primer <400> 23 tagactttgt gtctactyyt ctc 23 <210> 24 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha62F primer <400> 24 tctcaactaa acgaaatttt t 21 <210> 25 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Alpha252F primer <400> 25 aagcaamgtt ytgtckttgt gg 22 <210> 26 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Alpha950F primer <220> <221> modified_base <222> (6) <223> i <220> <221> modified_base <222> (9) <223> i <220> <221> modified_base <222> (18) <223> i <400> 26 ggttcnccnk ttatkacnaa tggt 24 <210> 27 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Alpha1421F primer <400> 27 ggtgtyatwg ayattagt 18 <210> 28 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha2981F primer <400> 28 ggtggyaarr twtcwttytc tga 23 <210> 29 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Alpha3940F primer <220> <221> modified_base <222> (4) <223> i <400> 29 trtngatggt ttyggtgt 18 <210> 30 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha4358F primer <220> <221> modified_base <222> (18) <223> i <400> 30 tttaatgttg twggkccncg 20 <210> 31 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha4904F primer <400> 31 tayatgtgyg ttktrccatc 20 <210> 32 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Alpha5385F primer <400> 32 ayaataattg ttgggtkaat gc 22 <210> 33 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha5546F primer <400> 33 aagggtgatg ctgarga 17 <210> 34 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha6842F primer <400> 34 tatgttaatg cwaatggtgg t 21 <210> 35 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha7517F primer <400> 35 gcktgytgta tgcgygc 17 <210> 36 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha8201F primer <400> 36 ggtggtraya atgtttattg yta 23 <210> 37 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha8432F primer <400> 37 tggtwtgtta aygatggamg tgt 23 <210> 38 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha8933F primer <400> 38 garagtgctg cwgcwgg 17 <210> 39 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Alpha8961F primer <400> 39 twgayatkcg ttcttatga 19 <210> 40 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha9077F primer <400> 40 taycgytgyg cttgttatgc 20 <210> 41 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha9203F primer <400> 41 atggcrcarc crtctgg 17 <210> 42 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha10476F primer <400> 42 tggcgytttk ctaraga 17 <210> 43 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha10814F primer <400> 43 aartayatgr twgctaatgg 20 <210> 44 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha11113F primer <400> 44 taarcayagt gattttggwc t 21 <210> 45 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha11189F primer <220> <221> modified_base <222> (3) <223> i <400> 45 tcntcwtttg ttrgtatgcc 20 <210> 46 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha11360F primer <400> 46 agratggctg aacaagctgc 20 <210> 47 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha11507F primer <400> 47 gcacgtratg gtgttgt 17 <210> 48 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha11624F primer <220> <221> modified_base <222> (9) <223> i <400> 48 taygckggng ttgtttggac 20 <210> 49 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha12179F primer <400> 49 gctgttarac awggygcaaa acc 23 <210> 50 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Alpha12255F primer <400> 50 ctggtarygg tcargctat 19 <210> 51 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha12532F primer <400> 51 tctagtgcmg ctcgactaga 20 <210> 52 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha12717F primer <400> 52 ggamcacgag cartccatgt a 21 <210> 53 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha13477F primer <400> 53 gtktttatwg atggtgtwcc 20 <210> 54 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Alpha13816F primer <400> 54 ggtgatgctg cwrttaaaga ttttga 26 <210> 55 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha13868F primer <400> 55 cttaataara gygctggktg gcc 23 <210> 56 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> Alpha14036F primer <220> <221> modified_base <222> (6) <223> i <400> 56 caraanggtg atgctgc 17 <210> 57 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha14220F primer <400> 57 cttaataara gygctgkttg gcc 23 <210> 58 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha14742F primer <400> 58 ggtacractt cwggtgaygc 20 <210> 59 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha15343F primer <400> 59 cckatgttgt gyactaartg 20 <210> 60 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha15532F primer <220> <221> modified_base <222> (12) <223> i <400> 60 gctggwaatr tntttggttt 20 <210> 61 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Alpha16114F primer <220> <221> modified_base <222> (15) <223> i <400> 61 acaatacagg gtccncctgg tagtgg 26 <210> 62 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha16561F primer <400> 62 cgwatgtgtg cwataggmcc 20 <210> 63 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha17266F primer <400> 63 cctggyagtc ayagtttgtt 20 <210> 64 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha17320F primer <400> 64 ggttggttrg gwatggatgt 20 <210> 65 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha17989F primer <220> <221> modified_base <222> (18) <223> i <400> 65 tttaaacayg cwttgggntg tga 23 <210> 66 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha18574F primer <400> 66 tttgawkktt ataatttgtg gca 23 <210> 67 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha18874F primer <400> 67 ttrtgggatt atgaagcwga 20 <210> 68 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha19333F primer <400> 68 tatggtgatg tktcwaaaac tac 23 <210> 69 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha19735F primer <400> 69 tgtaatttrt ataattaygg tgc 23 <210> 70 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha20047F primer <400> 70 tcwgatatgt aygatggyag 20 <210> 71 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha20829F01 primer <400> 71 tgyttatggt ctgtttctgg 20 <210> 72 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha20829F02 primer <400> 72 tgyttatggc ctgtacctgg 20 <210> 73 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Alpha20829F03 primer <220> <221> modified_base <222> (7) <223> i <400> 73 tgyytcnggc ctgtwcctgg 20 <210> 74 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Alpha20846F01 primer <400> 74 ggttttgtyt attttaaygg tactgg 26 <210> 75 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Alpha20846F02 primer <400> 75 ggttttgtyt attttaaygc cactgg 26 <210> 76 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha22436F primer <400> 76 ctyaagcagt ayacttctgc ttg 23 <210> 77 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Alpha22457F primer <400> 77 tgtaaaacya twgaagaygc ctta 24 <210> 78 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Alpha22789F primer <400> 78 tgatgcwgar cgwatggc 18 <210> 79 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Alpha23909F primer <220> <221> modified_base <222> (6) <223> i <400> 79 tggccntggt gggtktgg 18 <210> 80 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Alpha24066F primer <400> 80 tcaactaaac ttccttatta yga 23 <210> 81 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Alpha25053F primer <220> <221> modified_base <222> (9) <223> i <400> 81 gttggaatnt watyctaac 19 <210> 82 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha25287F01 primer <400> 82 gtttcagact tttccgwcgt g 21 <210> 83 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Alpha25287F02 primer <400> 83 gtttcagact ttggcgccgt g 21 <210> 84 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Alpha25679F primer <220> <221> modified_base <222> (12) <223> i <400> 84 ttgcttcatt tnwtctaaac traac 25 <210> 85 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Alpha25980F primer <400> 85 ggtgtygtyt gggttgct 18 <210> 86 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Alpha27065F primer <400> 86 tagtcttaya cacaatggta rgcc 24 <210> 87 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Alpha27168F primer <400> 87 tggagtamty aaagatcgc 19 <210> 88 <211> 27317 <212> DNA <213> Human alphacoronavirus 229E | NC_002645: 1 <400> 88 acttaagtac cttatctatc tacagataga aaagttgctt tttagacttt gtgtctactt 60 ttctcaacta aacgaaattt ttgctatggc cggcatcttt gatgctggag tcgtagtgta 120 attgaaattt catttgggtt gcaacagttt ggaagcaagt gctgtgtgtc ctagtctaag 180 ggtttcgtgt tccgtcacga gattccattc tacaaacgcc ttactcgagg ttccgtctcg 240 tgtttgtgtg gaagcaaagt tctgtctttg tggaaaccag taactgttcc taatggcctg 300 caactgtgtg acacttgccg tagcaagtga ttctgaaatt tctgcaaatg gctgttctac 360 tattgcgcaa gccgtccgcc gttatagcga ggccgctagc aatggtttta gggcatgccg 420 atttgtttca ttagatttgc aggattgcat cgttggcatt gcagacgata catatgttat 480 gggtctgcat ggcaatcaga cgttgttttg caacataatg aaattttctg accgtccttt 540 tatgcttcat gggtggttgg ttttttccaa ttcaaattac cttttggagg aatttgatgt 600 tgtcttcggt aagagaggtg gtggtaatgt gacatacact gaccagtatc tctgtggcgc 660 cgatggcaaa cctgttatga gtgaagattt atggcagttt gttgaccatt tcggtgagaa 720 cgaagaaatt atcatcaatg gtcatactta cgtttgtgct tggcttacta agcgtaagcc 780 cttagattac aaacgtcaga acaaccttgc cattgaagag attgaatatg tgcatggtga 840 tgctttgcat acactacgca atggttctgt tcttgaaatg gctaaggaag tgaagacatc 900 tagtaaagtt gtgttaagcg atgctcttga caaactttac aaagtctttg gttctcctgt 960 tatgacaaat ggttccaaca tcctagaggc ctttactaaa cctgtgttta ttagtgcatt 1020 agttcaatgt acttgtggta ccaagtcttg gtctgttggt gattggaccg gttttaaatc 1080 ctcttgttgc aacgtgatca gtaataaact gtgtgttgtt cccggtaatg ttaaacctgg 1140 tgatgctgtg attaccactc agcaagctgg tgctggtatt aagtattttt gtggcatgac 1200 tcttaagttt gttgcaaata ttgaaggtgt ctctgtttgg agagtgattg ctcttcagag 1260 tgtggattgc tttgttgctt cttccacttt tgtagaagag gaacatgtta atagaatgga 1320 tacattctgc ttcaatgtac gcaatagtgt tactgatgag tgtcgtctgg ccatgttggg 1380 tgctgaaatg actagtaatg tcagaagaca agttgcttca ggtgtcatag acattagtac 1440 cggttggttt gatgtttatg atgacatctt tgctgaaagc aaaccatggt ttgttcgcaa 1500 ggctgaagac atttttggcc cttgttggtc cgctcttgtt tctgcactta aacaacttaa 1560 agtcactaca ggtgaacttg tgagatttgt taagtctatt tgcaattcag ctgttgctgt 1620 cgtgggtggt actatacaaa ttctcgctag tgtgcctgag aagtttttga atgcgtttga 1680 cgtgtttgtc acagctattc aaactgtctt tgactgtgct gttgaaactt gtactattgc 1740 cggtaaagca tttgacaagg tttttgacta tgtcttgctt gataatgcgc ttgtaaaact 1800 tgtcaccaca aagcttaagg gtgttcgtga acgtggcctt aataaagtta agtatgcaac 1860 agttgttgtt ggttccactg aagaagttaa atcttcacgt gttgaacgta gcactgctgt 1920 acttacaatc gccaacaatt attccaaact ttttgatgaa gggtatactg ttgtaattgg 1980 cgatgtggcg tactttgtta gtgacggcta cttccgtctt atggccagtc caaatagtgt 2040 gttgactact gcagtctata aaccattgtt tgcttttaat gtgaatgtta tgggtactag 2100 acctgaaaaa tttccaacca ctgtgacttg tgaaaattta gagtctgttg ttttgtttgt 2160 taatgacaaa attactgaat tccaattgga ttacagtatt gatgtcattg ataatgaaat 2220 aattgtcaaa cctaatatca gcctatgtgt tccactttat gtgagagact atgttgacaa 2280 atgggatgat ttttgcagac aatatagtaa cgagtcttgg tttgaggatg attacagggc 2340 ttttatcagt gttttggaca tcactgatgc tgctgtgaaa gctgcagagt ctaaagcttt 2400 cgttgatact attgttccac cttgcccatc tattttgaaa gttatagatg gaggcaaaat 2460 atggaatggt gttattaaaa atgttaactc tgttagagac tggcttaagt ctttgaagtt 2520 aaatctcaca caacagggtt tgcttggaac atgtgcaaag cgttttaaac gttggcttgg 2580 cattttgcta gaggcctata atgcgttttt agacactgtg gtttctactg ttaaaattgg 2640 tggcttgacc tttaaaacat atgcttttga taaaccttac attgtgatac gtgatatcgt 2700 gtgtaaggtt gaaaataaaa cagaagcaga atggattgag ctttttccac ataatgacag 2760 gattaagtct tttagtactt tcgagagtgc ttacatgcca attgcagacc ctacacattt 2820 tgacattgaa gaagttgaac ttttagatgc agagtttgta gaaccaggct gtggtggtat 2880 tttggcagta atagatgagc acgtctttta taagaaggat ggtgtttatt atccatcaaa 2940 tggtactaac attctacctg ttgcatttac aaaagccgct ggtggtaaag tttcattttc 3000 tgatgacgtt gaagtaaaag acattgaacc tgtttacaga gtcaagcttt gctttgagtt 3060 tgaagatgaa aaacttgtag atgtttgtga aaaggcaatt ggcaagaaaa ttaaacatga 3120 aggtgactgg gatagctttt gtaagactat tcaatcagca ctttctgttg tttcttgcta 3180 tgtaaatcta cctacttatt acatttatga tgaagaaggc ggtaatgact tgagtttgcc 3240 cgttatgatt tctgaatggc ctctttctgt tcaacaagct caacaagaag ctactttacc 3300 tgatattgct gaggatgttg ttgaccaagt tgaagaagtc aatagcattt ttgacattga 3360 gacagtggat gttaaacatg atgtgagtcc ttttgaaatg ccatttgaag agttaaatgg 3420 tttaaagata ctcaaacaat tggataacaa ctgctgggtt aactcagtta tgttacaaat 3480 acaattaact ggtatacttg atggtgacta tgctatgcag ttttttaaaa tgggccgagt 3540 tgccaagatg attgaacgct gctacactgc tgagcaatgt atacgtggtg ctatgggtga 3600 tgttggtttg tgtatgtata gactgcttaa agacttacac actggtttta tggttatgga 3660 ttataaatgt agttgtacca gtggtaggct tgaagaatcg ggagctgttt tgttttgtac 3720 gcccactaag aaggcgtttc cttatggtac ttgtctaaat tgtaacgcac ctcgcatgtg 3780 tacaattagg cagttacaag gtaccataat atttgtgcaa caaaaaccag aacctgttaa 3840 tcctgtttct tttgttgtta aaccagtctg ctcatcaatt tttcgtggtg ctgtgtcttg 3900 tggtcattac cagactaaca tctattcaca aaatttgtgt gtggatggtt ttggtgttaa 3960 caagattcag ccctggacaa atgatgcact taatactatt tgtattaagg atgcagatta 4020 taatgcaaaa gttgaaatat ctgttacacc aattaaaaat acagttgata caacacctaa 4080 ggaagaattt gttgttaaag agaagttgaa cgccttcctc gttcatgaca atgtagcttt 4140 ctaccaaggt gatgttgata ctgttgttaa tggtgttgac tttgacttta ttgtaaatgc 4200 tgctaatgag aaccttgctc atggtggagg acttgccaaa gctttagatg tgtacactaa 4260 aggtaaactt caacgtttat ctaaagaaca cattggatta gcgggtaaag taaaagttgg 4320 tacaggagtt atggttgagt gtgatagcct tagaattttt aatgttgttg gtccacgcaa 4380 gggtaaacat gaacgtgatt tactcataaa agcttacaac actattaata atgaacaagg 4440 cacaccttta acaccaattt tgagctgtgg tatttttggt atcaaactcg aaacttcatt 4500 agaagttttg cttgatgttt gtaatacaaa agaagttaaa gtttttgttt atacagacac 4560 agaggtttgt aaggttaagg attttgtgtc tggtttagtg aatgttcaaa aagttgagca 4620 acctaaaata gaaccaaaac cagtgtccgt aattaaagtt gcacccaagc cttacagggt 4680 agatggtaaa tttagttact ttacagaaga cttgttgtgt gtcgctgatg acaaacccat 4740 tgttttgttt actgactcta tgcttacttt ggatgaccgt ggtttagctc tagacaatgc 4800 acttagtggt gtgcttagtg ctgctattaa ggattgtgtt gacataaata aagctatacc 4860 ttctggtaat cttattaagt ttgatatagg ttctgttgtt gtctacatgt gtgttgtgcc 4920 atccgaaaag gacaaacatt tagataataa tgttcaacga tgcacacgta agttgaatag 4980 acttatgtgt gatatagttt gtactatacc agctgactac atcttgccat tggtgttgtc 5040 tagtttgact tgtaatgttt cttttgtagg tgaacttaaa gctgctgaag ctaaagttat 5100 aactataaag gtgacagagg atggtgttaa tgttcatgat gtgaccgtga caacagacaa 5160 gtcatttgaa caacaagttg gtgttattgc tgataaggac aaagatcttt ctggtgcagt 5220 accaagtgat cttaacacat ctgaattgct tactaaagca atagatgttg attgggtcga 5280 attttatggc tttaaagatg ctgttacttt tgcaacagtt gatcatagtg cttttgccta 5340 tgaaagtgct gttgttaatg gtattagagt gttaaaaact agtgataata attgttgggt 5400 gaatgctgtt tgtattgcac tacagtattc gaaaccccat tttatttcac aaggtcttga 5460 tgctgcgtgg aataaatttg ttttaggcga tgttgaaatt tttgttgcat ttgtttacta 5520 tgttgcaaga ctaatgaaag gtgacaaggg tgatgctgaa gacactttga ctaagttgtc 5580 taagtatctt gctaatgaag ctcaagttca attagaacat tatagttctt gtgttgaatg 5640 tgatgctaaa tttaaaaact ctgttgcatc tatcaattct gctatagttt gtgctagtgt 5700 caaacgtgat ggtgtgcaag ttggttattg tgtccatggt attaagtact attcacgtgt 5760 tagaagtgtt agaggtagag ctattatagt cagtgtcgaa cagcttgaac cgtgtgctca 5820 gtctagactt ttgagtggtg ttgcttatac tgctttttct ggacctgttg acaaaggtca 5880 ttatactgtt tatgatactg caaagaaatc aatgtatgat ggtgatcgtt ttgttaaaca 5940 tgatctttct ctgctgtctg tcacatcagt tgttatggtt ggtggttatg ttgcacctgt 6000 taatacagtg aaacctaaac cagtcattaa tcaacttgat gaaaaggcac agaagttctt 6060 tgattttggt gattttttga ttcataattt tgttattttt ttcacatggt tattgagtat 6120 gtttactttg tgtaaaactg cagtaactac aggtgatgtt aaaataatgg ccaaagcacc 6180 acaaaggacg ggtgttgttt taaaacgtag tcttaaatat aacttaaaag cgtcagcagc 6240 tgttcttaaa tctaagtggt ggctgcttgc taagtttacg aaactactgt tactcatata 6300 tacattgtac tcagtagttt tgctttgtgt acgttttgga ccgtttaatt tttgtagtga 6360 gactgttaat ggttatgcta agtcaaactt tgtcaaggat gattactgtg atggttcatt 6420 gggctgcaag atgtgtcttt ttggttacca agagttaagt caatttagcc atttggatgt 6480 tgtgtggaag catataacag accctttgtt tagtaatatg caacctttca ttgtcatggt 6540 tttgctgctt atatttggtg acaattattt gagatgcttc ttgctgtatt ttgttgctca 6600 gatgataagc acagttggtg tttttctagg ttacaaggaa acaaattggt tcttgcactt 6660 tattccattt gatgttattt gtgatgaact gcttgtcact gttattgtta ttaaggttat 6720 ttcttttgtc agacatgtgc tttttggttg tgaaaaccca gattgcattg cgtgttctaa 6780 gagtgctaga cttaagagat tccctgttaa cacaattgtc aatggtgtgc aacgttcatt 6840 ttatgttaat gcaaatggtg gtagtaagtt ttgtaagaaa catagatttt tctgtgttga 6900 ttgtgactct tatggttatg gcagcacgtt tataacaccc gaagtttcta gagaacttgg 6960 taacattacc aaaacaaatg tgcaaccaac aggcccagcg tatgtcatga ttgacaaagt 7020 ggagtttgaa aatggttttt acagattgta ttcctgtgaa acattttggc gttacaactt 7080 tgatataact gaaagcaagt attcttgcaa agaggttttt aaaaattgta atgttttgga 7140 tgatttcatc gtgtttaaca ataatgggac caatgtaacg caggttaaaa atgctagtgt 7200 ttacttttca cagttgttgt gtaggcccat taaattagtt gacagtgaac ttttgtccac 7260 tttgtcagtt gattttaatg gtgtcttaca caaggcatac attgatgtac tacgtaatag 7320 ctttggtaaa gatcttaatg ctaatatgtc tttagccgag tgcaagagtg ctttaggcct 7380 gtctattagt gatcatgaat ttactagtgc tatttctaat gcacatcgtt gtgacgtgtt 7440 gttatctgat ttgtcattta acaactttgt cagttcgtat gctaaacctg aggaaaaatt 7500 atcagcttat gacttggcgt gttgtatgcg tgcaggtgct aaggttgtta atgccaatgt 7560 tctgacaaag gaccaaactc ctattgtttg gcatgcaaag gattttaaca gtctttctgc 7620 tgaaggtcgc aagtatattg taaaaactag caaagctaag ggtttgactt tcttgttgac 7680 aattaatgaa aaccaagctg tcacgcaaat acctgcaact agcattgttg ctaagcaagg 7740 tgctggtgat gctggccatt cattaacatg gctgtggcta ctgtgtggtc ttgtgtgttt 7800 gattcaattc tacttgtgct ttttcatgcc ctattttatg tacgatatcg tgagtagttt 7860 tgagggttat gattttaagt atatagaaaa tggtcagttg aagaattttg aagcgccact 7920 taaatgcgtc agaaacgttt ttgaaaactt tgaggactgg cattatgcta agtttggctt 7980 cacaccttta aacaagcaaa gctgtcctat tgtagttgga gtttctgaaa ttgttaatac 8040 tgtcgctggc attccatcta atgtgtatct tgttggtaaa actttaattt ttacactaca 8100 agctgctttt ggtaatgctg gtgtttgtta tgacattttt ggagtcacaa cacctgaaaa 8160 gtgcattttt acttctgctt gtactagatt agaaggtttg ggtggtgaca atgtttattg 8220 ttataacaca gcgcttatgg aaggttcttt gccttacagt tcaatacaag ctaatgcata 8280 ttataaatat gacaatggca attttattaa gttgccagaa gttattgcac aaggctttgg 8340 ttttagaaca gtgcgtacta ttgccaccaa atactgccgc gtaggtgaat gtgttgaatc 8400 caatgcaggt gtgtgttttg gctttgacaa gtggtttgtt aacgatggac gtgttgccaa 8460 tggttacgtt tgtggtactg gtttgtggaa ccttgtattt aacatacttt ccatgttttc 8520 atcttcattc tctgttgctg caatgtcagg tcaaatttta cttaattgtg cattaggtgc 8580 ttttgctatt ttttgttgtt ttcttgtgac aaagtttaga cgcatgtttg gtgacctttc 8640 tgtaggtgtt tgcactgttg ttgtggctgt tttgcttaac aatgtctctt acattgtaac 8700 tcagaattta gtaacaatga ttgcttatgc catattgtat ttctttgcta ctagaagctt 8760 acgctatgca tggatttggt gtgctgcata tttaattgcg tatatttctt ttgctccatg 8820 gtggttgtgt gcttggtact ttcttgctat gttgacaggt ttgttaccta gtttgctgaa 8880 gcttaaagtt tcgacaaatc ttttcgaagg tgacaaattt gtaggtacat ttgaaagtgc 8940 tgctgcagga acatttgtca ttgacatgcg ttcttatgag aaacttgcta atagcatctc 9000 tccagaaaag ttgaaaagtt atgctgctag ctataataga tataagtact atagtggtaa 9060 tgcaaatgaa gctgattact gttgcgcttg ttatgcctat ttagcaaaag caatgttgga 9120 cttttcgcgt gatcataatg acatcttgta cacacctccg actgtcagtt atggttctac 9180 attacaggct ggtttgcgca aaatggcaca accatctggc tttgtggaga aatgtgttgt 9240 ccgtgtctgc tatggaaaca ctgtgttgaa tgggttgtgg cttggtgata ttgtttattg 9300 cccacgtcat gttatcgcat ctaacacaac ttctgctata gattatgatc acgaatatag 9360 tattatgcgg ttgcataatt tttctataat atctggtaca gcatttcttg gtgttgtagg 9420 tgctactatg catggagtaa ctcttaaaat taaggtttca cagactaaca tgcacacacc 9480 tagacattct tttagaacac taaaatctgg tgaaggtttt aacatcttag catgctatga 9540 tggttgtgct caaggtgttt ttggtgtgaa catgagaact aattggacta tccgtggttc 9600 atttattaat ggtgcgtgtg gttcccctgg ctacaatctt aaaaatggcg aggtggaatt 9660 tgtttatatg catcaaattg aactcggaag tggtagccat gtaggttcta gctttgatgg 9720 tgttatgtat ggtggttttg aagaccaacc taatcttcaa gttgaatctg caaaccagat 9780 gttaacagtt aatgtggttg catttcttta tgctgctata ttgaatggtt gcacatggtg 9840 gcttaaaggt gaaaaattgt ttgtggagca ttataatgag tgggcacagg ctaatggttt 9900 cacagctatg aatggtgaag acgctttttc cattcttgct gctaaaactg gtgtctgtgt 9960 ggaaagatta cttcatgcta ttcaagtttt gaataatggc tttggtggta aacaaatttt 10020 gggttattct agtctcaatg atgagttcag tattaatgaa gttgtcaaac aaatgtttgg 10080 tgttaacctg caaagtggta aaaccactag tatgtttaaa tccataagct tatttgctgg 10140 cttctttgtc atgttctggg ctgaattatt tgtttatacc accactattt gggttaaccc 10200 tggttttctt actccgttta tgattttgct tgttgctttg tcactctgtc ttacatttgt 10260 tgttaaacat aaggttttgt ttttgcaagt gtttttgttg ccttcaatta ttgtggctgc 10320 tattcaaaac tgtgcttggg actaccatgt tacaaaggtg ttggcagaga agtttgatta 10380 taatgtttct gttatgcaaa tggacatcca gggttttgtt aacattttta tttgtctttt 10440 tgttgcactg ttgcatactt ggcgctttgc taaagagcgt tgtacacatt ggtgcactta 10500 tttgttctca ctcattgctg ttttatacac tgcattgtat agttatgact acgttagttt 10560 gctggttatg ctactttgtg caatttctaa tgaatggtat attggtgcta ttatttttag 10620 aatttgtcgt tttggtgttg catttttacc agtggaatac gtgtcttact ttgatggtgt 10680 taaaactgtg ctgttgtttt acatgttgtt aggctttgtt agctgtatgt actatggttt 10740 gttgtactgg attaacaggt tctgtaagtg cacattaggt gtttatgatt tctgtgttag 10800 tccagccgaa tttaagtata tggttgctaa tggtttgaat gcaccaaatg gcccttttga 10860 tgcgctcttt ctgtctttta aactaatggg tattggcggt cctagaacca ttaaagtttc 10920 tactgtacag tctaaattga ctgatcttaa gtgcacaaac gtcgttctaa tgggcatttt 10980 gtctaacatg aacatagctt ctaattcaaa ggagtgggca tattgtgttg aaatgcacaa 11040 taaaataaac ttgtgtgacg accctgaaac tgctcaagag ttattgctgg cgttgttggc 11100 ctttttcttg tctaagcata gtgattttgg tcttggtgat cttgtcgatt cttattttga 11160 gaacgactcc attttgcaaa gtgttgcatc ttcttttgtt ggtatgccat cttttgttgc 11220 atatgaaaca gcaagacaag agtatgaaaa tgctgttgca aatggttcct caccacaaat 11280 aatcaaacaa ttgaagaagg ctatgaatgt tgcaaaagct gagtttgaca gggaatcatc 11340 tgttcaaaag aaaattaaca gaatggctga acaagctgct gcagctatgt acaaagaagc 11400 acgtgctgtt aatagaaaat caaaagttgt tagtgccatg catagtttac tctttggcat 11460 gctccgacgt ttggacatgt ctagtgttga cactatcctt aatatggcac gtaatggtgt 11520 tgtccctctt tccgttatcc ctgctacttc tgcagccagg ctcgtcgtcg tagtaccaga 11580 tcatgattca tttgtgaaaa tgatggtaga tggttttgtg cactacgctg gtgttgtttg 11640 gacattacag gaagttaagg ataatgatgg taagaatgtg catcttaaag atgttacaaa 11700 ggaaaaccag gaaatacttg tttggcctct gattttgact tgtgaacgtg tcgttaaatt 11760 gcagaacaat gaaataatgc cgggcaagat gaaggtcaag gccaccaaag gtgaaggtga 11820 tggaggcatt actagtgaag gtaatgctct atacaacaat gaaggtggac gtgcattcat 11880 gtatgcatat gtgactacga agcctggcat gaagtatgtt aaatgggaac atgactctgg 11940 tgtggttaca gttgaattgg aaccaccttg cagatttgtt atagacacac ctactggacc 12000 ccaaattaag tatctttatt ttgttaagaa tcttaacaat ttaaggagag gtgctgtttt 12060 gggttacatt ggtgccactg tgagattgca agctggcaaa cagactgagt ttgtttcaaa 12120 ctcccattta ttaacacatt gttcttttgc tgttgaccca gctgcagcct atcttgatgc 12180 tgttaaacaa ggcgcaaaac ctgttggcaa ttgtgtaaag atgttgacta atggttctgg 12240 tagcggtcag gctattactt gtaccattga ttccaacact acgcaggaca catatggtgg 12300 cgcgtctgtt tgtatttatt gcagagcaca tgttgcacat ccaaccatgg acggtttttg 12360 tcagtacaaa ggcaagtggg tacaagtgcc tataggtaca aatgacccta taagattttg 12420 tcttgaaaat actgtttgta aagtttgtgg ttgttggctt aatcatggct gtacatgtga 12480 ccggactgct atccaaagtt ttgataacag ttatttaaac gagtccgggg ctctagtgcc 12540 gctcgactag agccctgtaa tggtacagac atagattact gtgtccgtgc atttgacgtt 12600 tacaataaag atgcgtcttt tatcgggaaa aatctgaagt ccaattgtgt gcgcttcaag 12660 aatgtagata aggatgacgc gttctatatt gttaaacgtt gcattaagtc agttatggac 12720 cacgagcagt ccatgtataa cttacttaaa ggctgtaatg ctgttgctaa gcatgatttc 12780 tttacttggc atgagggcag aaccatttat ggtaatgtta gtagacagga tcttactaaa 12840 tacaccatga tggatttgtg cttcgctctg cgtaactttg atgaaaaaga ctgtgaagtt 12900 tttaaggaga tattggttct tactggttgt tgtagtactg attactttga aatgaagaat 12960 tggtttgacc ccatagaaaa tgaggacata caccgtgtgt atgctgcttt aggtaaggta 13020 gttgcaaatg caatgcttaa gtgtgttgct ttttgcgacg aaatggtgct caaaggagtt 13080 gttggtgttt tgaccttaga caaccaagat cttaatggga atttctatga cttcggtgac 13140 tttgtattgt gtcctcctgg aatgggaata ccctactgca cgtcatacta ttcttatatg 13200 atgcctgtta tgggtatgac taattgttta gctagtgagt gctttatgaa aagtgacatc 13260 tttggtcaag acttcaaaac ttttgatttg ttgaaatatg atttcacaga acataaggag 13320 gttttgttta acaagtactt taagtattgg ggacaggatt atcatcctga ttgtgttgat 13380 tgccatgacg agatgtgtat tttgcattgt tcaaatttta acacactctt cgcaaccaca 13440 attccaaaca cggcttttgg acctctatgc agaaaagtgt ttattgatgg tgtacccgta 13500 gttgctactg ctggttacca ctttaaacaa ttaggacttg tgtggaacaa agatgttaac 13560 actcattcta ccagacttac tattactgaa ctcttacagt ttgtgacaga tccaacgctt 13620 atagttgcgt catcgcctgc cttggtggat aaacgcactg tttgtttttc tgtcgctgct 13680 ttgagtacag gattaacatc ccaaacagta aaacctggcc attttaataa ggagttttat 13740 gacttcttac gttctcaggg gtttttcgat gagggttcag aattaacatt gaagcatttc 13800 ttttttacac aaaagggtga tgctgcaatt aaagattttg attattatcg ttacaacaga 13860 cctactatgc tggatattgg acaagctcgc gtagcatatc aagtggcagc tcgctatttt 13920 gactgttacg agggtggctg tattacatct agagaggttg ttgttacaaa ccttaataaa 13980 agcgctggtt ggccccttaa taagtttggt aaagctggtt tatattatga gtctattagt 14040 tatgaggaac aagatgctat tttttcatta acaaagcgta atattctccc tactatgact 14100 cagttaaatc ttaaatacgc catatctggt aaggaacgcg cacgtacagt gggtggcgtc 14160 tctttattag ctactatgac tacaagacag tttcatcaga aatgtctgaa atccatagta 14220 gctaccagaa atgccaccgt tgttatcggc actaccaagt tttatggcgg gtgggataat 14280 atgttaaaga acctgatggc cgatgttgat gatcctaaat tgatgggatg ggactatcct 14340 aagtgtgata gagctatgcc ctcaatgatt cgtatgttgt cggctatgat cttaggttct 14400 aagcatgtca catgttgtac ggctagtgat aaattttata gacttagtaa tgagcttgct 14460 caagttttga ccgaggttgt ttattcaaat ggtgggtttt attttaaacc tggtggtaca 14520 acttctggtg atgcaactac agcctatgcc aattctgtct ttaatatatt tcaggctgta 14580 agttctaaca ttaattgcgt tttgagcgtt aactcgtcaa attgcaataa ttttaatgtt 14640 aagaagttac agagacaact ttatgataat tgctatagaa atagtaatgt tgatgaatct 14700 tttgtggatg acttttatgg ttatttgcaa aagcattttt ctatgatgat tctttctgat 14760 gatagtgttg tgtgctataa taaaacttat gctggacttg gttacattgc tgatattagt 14820 gcttttaaag ccactttgta ttatcagaat ggtgtgttta tgagtacagc taagtgttgg 14880 actgaggaag atctttctat aggacctcat gaattttgct cacagcacac tatgcagatt 14940 gtagatgaaa atggtaagta ttatctacca tatccagatc ctagccgtat tatttctgct 15000 ggtgtttttg tggatgacat cactaagact gatgctgtca ttcttttgga acgctatgtt 15060 tctctggcta tagatgccta cccattgtct aagcatccta aacctgagta caggaaggtg 15120 ttttacgcat tgttagactg ggtcaaacat ctcaacaaga ctcttaacga aggtgttttg 15180 gagtcttttt ctgttacact tttagatgaa catgagtcta agttttggga tgaaagcttt 15240 tatgctagta tgtatgagaa gtctacagta ttacaagctg ctggtctttg tgtagtatgt 15300 ggttctcaaa cagttctaag atgcggtgat tgtttacgca gaccgatgtt gtgcactaag 15360 tgcgcctatg atcatgtgtt tggcactgat cataagttca ttttagctat tacaccatat 15420 gtgtgtaaca catctggctg caatgtaaat gacgttacaa aactgtatct tggaggtttg 15480 aattattact gtgtagacca caaaccacat ctttcattcc cactgtgttc agttggtaat 15540 gtctttggtt tgtacaaaag ttctgctttg ggttccatgg acattgatgt ctttaacaaa 15600 ctttctacct ctgattggtc tgacattcgc gactacaagc ttgctaatga tgcaaaagag 15660 tcactaaggt tgtttgcagc tgaaacggtc aaggctaaag aggaaagtgt taagtcatca 15720 tacgcttatg ctaccctaaa ggagattgta ggtcctaagg aacttttgct cttatgggaa 15780 agtggaaaag ccaaaccacc gttaaaccgt aattctgttt ttacatgctt ccaaattaca 15840 aaagactcca agtttcaagt tggtgagttt gtgtttgaga aagtagatta cggttctgat 15900 acggttactt acaaatccac tgctactact aagttagtac caggtatgtt gtttattttg 15960 acttctcata atgttgctcc acttagagcg ccaacaatgg caaaccagga gaaatattct 16020 accatttaca agttgcaccc atcatttaat gttagtgatg cttatgcaaa tcttgtacct 16080 tattaccaac ttattggcaa acagcgtatc accacaatac agggtcctcc tggtagtgga 16140 aaatcgcatt gttctattgg tattggtgtg tattaccctg gagcgaggat cgtgttcacc 16200 gcttgttctc acgctgctgt tgattcgctc tgtgcaaaag ctgtcacagc ctatagtgtt 16260 gataagtgta cacgtattat tcctgcacgt gccagagttg agtgttatag tggttttaaa 16320 cctaacaata atagtgcaca atacgtgttt agtactgtta atgcgttacc tgaagttaat 16380 gcagacattg ttgtcgtgga tgaggtgtct atgtgcacta actatgactt gtctgtgatt 16440 aaccagcgta tatcatataa acacattgta tatgttggtg atcctcaaca gcttccagct 16500 cctagagttc ttatctctaa aggtgttatg gaaccaattg actataatgt tgtgacacaa 16560 cgtatgtgtg ctataggacc cgatgtcttt ttacacaagt gttacagatg tcctgctgaa 16620 atagttaaca ctgtttcaga gcttgtttat gaaaacaagt ttgtacctgt caaagaagct 16680 agtaagcagt gcttcaaaat ctttgaacgc ggtagtgttc aggtagacaa tggctccagt 16740 ataaataggc gtcaacttga tgttgttaag cgatttatac ataaaaactc cacatggagc 16800 aaggctgtgt ttatctcacc ttacaatagt caaaattatg tagctgccag gcttttaggc 16860 ttacaaactc agacagtgga ttctgctcaa ggtagtgaat atgactatgt tatattcgca 16920 cagacatcag atactgctca tgcctgtaat gccaatcgtt ttaacgttgc cattactaga 16980 gcaaagaaag gtattttctg tattatgtct gacagaactt tgtttgatgc acttaagttc 17040 tttgaaatca ctatgacaga tttacagtct gaaagtagtt gtggtttgtt taaggattgt 17100 gcacgtaacc ctattgattt accaccaagt catgccacta cttatttgtc attgtctgat 17160 agatttaaga ctagtggtga cttggctgtt caaataggta acaacaatgt ttgtacctat 17220 gaacatgtga tttcatatat gggtttcagg tttgatgtta gcatgcctgg tagtcatagt 17280 ttgttctgta ctagagactt tgccatgcgt catgtcagag gttggttagg aatggatgtg 17340 gaaggtgcac atgtcacagg tgacaatgtt ggcactaatg tacctctaca agttggtttt 17400 tccaatggtg ttgattttgt agctcaacct gaaggttgtg ttctaacaaa cactggcagt 17460 gttgtaaaac ctgttcgtgc tcgtgcacca cctggagaac aatttactca cattgtacct 17520 ctgttacgca agggacaacc ttggagtgtg ttgagaaaac gtattgttca aatgatagca 17580 gattttcttg ctggctcatc tgatgtactg gtgtttgtac tttgggctgg cggtttagag 17640 ttgaccacta tgcgttattt tgttaagatt ggagctgtta aacattgcca atgtggtact 17700 gttgcaacat gctacaattc tgttagtaat gactattgtt gctttaaaca tgcattgggc 17760 tgtgactatg tttataatcc atatgtcata gatattcaac aatggggtta tgttggttca 17820 ctctccacta atcaccatgc aatttgtaat gttcatagaa atgagcatgt tgcttctggt 17880 gatgctatta tgactagatg tttggctgtg tatgactgct ttgttaagaa tgtggattgg 17940 tcaattacct accctatgat agctaatgaa aatgccataa acaagggcgg tcgcactgtg 18000 cagagtcata ttatgcgtgc tgctattaaa ttgtacaacc ctaaagcaat ccatgacatt 18060 ggtaatccta agggtattcg ttgtgctgta actgatgcca agtggtattg ttatgacaag 18120 aaccctatta attctaatgt gaaaacattg gagtatgatt acatgacaca tggccaaatg 18180 gatggcttgt gtttgttttg gaattgtaat gtggatatgt accctgaatt ctcaattgtt 18240 tgcaggtttg acacacgtac acgatctaca ttgaaccttg aaggtgtaaa tggtgggtca 18300 ttgtatgtca ataatcatgc atttcacact cctgcttatg ataaacgtgc tatggctaaa 18360 ttgaaaccag caccgttttt ctactatgac gacggttcat gtgaggttgt tcacgatcaa 18420 gttaactatg ttcctttgag agccactaat tgcattacca agtgtaatat tggtggtgct 18480 gtatgttcta agcacgctaa tctctataga gcatatgttg agtcatataa catttttact 18540 caagctggtt ttaatatttg ggttcctacc acgtttgatt gttataattt gtggcagaca 18600 ttcacagagg tcaatttaca aggtttagag aacattgctt ttaacgttgt taataaaggt 18660 tcatttgttg gtgctgatgg tgaattacca gtagccatta gtggtgataa agtgttcgta 18720 cgtgatggta acactgataa tttagtcttt gttaacaaaa catcactgcc tacaaacata 18780 gcatttgaac tttttgctaa gaggaaggtt ggtttaacac cacctctcag tattctcaaa 18840 aaccttggtg ttgtcgccac atataagttt gtcttgtggg attatgaagc tgagcgtccc 18900 ttgacaagct ttactaagtc tgtttgtggt tatacagact ttgcagagga tgtttgtact 18960 tgttacgata atagtataca aggttcatac gaacgtttta ctctgtcaac taatgctgtg 19020 ttattctctg ctactgctgt gaaaacaggt ggtaagagtt tgccggctat taaattgaat 19080 tttggaatgc ttaatggtaa tgcaattgct actgtcaaat cagaagatgg taacataaaa 19140 aatattaact ggtttgttta cgtacgcaaa gatggcaaac ctgttgatca ttatgatggt 19200 ttttataccc aaggtcgtaa tttacaagac tttttgcctc gcagcacaat ggaagaagac 19260 tttttgaaca tggatatagg cgtgtttatt caaaagtatg gtctagagga tttcaacttc 19320 gagcacgttg tgtatggtga tgtttcaaaa actactctag gcggtttaca cttgttgatt 19380 tcacaagtac gtctgagtaa aatgggcatc ttaaaggcag aggagtttgt ggcagcatct 19440 gacataacac tcaaatgttg tactgtgact tatcttaatg atcctagttc taagactgtt 19500 tgtacttaca tggatttgtt gttggatgat tttgtttctg tattgaagtc tttggatttg 19560 actgttgtat ccaaggttca tgaggtcata attgacaaca aaccatggag atggatgcta 19620 tggtgtaaag ataatgccgt tgctacattc tatcctcagt tgcagagtgc agaatggaaa 19680 tgcgggtatt ctatgcctgg tatttataag acacaacgta tgtgcttaga accatgtaat 19740 ttgtataatt atggtgcagg tttgaagttg cccagtggca ttatgttcaa tgttgttaaa 19800 tacactcaat tgtgtcaata ttttaacagt accacgttat gtgttcctca taatatgaga 19860 gtgttacact tgggtgctgg ctctgattat ggtgttgcac caggaactgc tgttcttaaa 19920 aggtggttgc cgcacgacgc aattgttgtt gacaacgatg ttgttgacta tgtgagtgac 19980 gctgatttta gtgttactgg tgattgtgca accgtttatt tggaagacaa gtttgacttg 20040 ttaatctctg atatgtacga tggcaggaca aaggcaattg atggtgaaaa tgtttcgaaa 20100 gaaggatttt tcacttacat ctatggtttc atttgtgaaa aacttgccat cggaggttcg 20160 attgctatta aagtaacaga gtatagctgg aataagaaat tgtatgaact tgtacaaaga 20220 ttttcttttt ggactatgtt ttgcacttct gttaatacgt catcatcaga agcctttgtt 20280 gtcggaatta actatcttgg tgatttcgca caaggacctt ttatagatgg taacataata 20340 cacgcaaatt atgtattttg gcgtaactcc actgttatga gtttgtccta caactctgtt 20400 ttagacctga gtaaatttaa ttgcaaacac aaagcgactg ttgttgtgca attaaaggat 20460 agtgatatta atgaaatggt gcttagtctt gttaggagtg gtaagttgct tgtaaggggt 20520 aatggcaagt gtttgagttt tagtaatcat ttagtctcaa ctaaataaaa tgtttgtttt 20580 gcttgttgca tatgccttgt tgcatattgc tggttgtcaa actacaaatg ggctgaacac 20640 tagttactct gtttgcaacg gctgtgttgg ttattcagaa aatgtatttg ctgttgagag 20700 tggtggttat ataccctccg actttgcatt caataattgg ttccttctaa ctaatacctc 20760 atctgttgta gatggtgttg tgaggagttt tcagcctttg ttgcttaatt gcttatggtc 20820 tgtttctggc ttgcggttta ctactggttt tgtctatttt aatggtactg ggagaggtga 20880 ttgtaaaggt ttttcctcag atgttttgtc tgatgtcata cgttacaacc tcaattttga 20940 agaaaacctt agacgtggaa ccattttgtt taaaacatct tatggtgttg ttgtgtttta 21000 ttgtaccaac aacactttag tttcaggtga tgctcacata ccatttggta cagttttggg 21060 caatttttat tgctttgtaa atactactat tggcaatgaa actacgtctg cttttgtggg 21120 tgcactacct aagacagttc gtgagtttgt tatttcacgc acaggacatt tttatattaa 21180 tggctatcgc tatttcactt taggtaatgt agaagccgtt aatttcaatg tcactactgc 21240 agaaaccact gatttttgca ctgttgcgtt agcttcttat gctgacgttt tggttaatgt 21300 gtcacaaacc tctattgcta atataattta ttgcaactct gttattaaca gactgagatg 21360 tgaccagttg tcctttgatg taccagatgg tttttattct acaagcccta ttcaatccgt 21420 tgagctacct gtgtctattg tgtcgctacc tgtttatcat aaacatacgt ttattgtgtt 21480 gtacgttgac ttcaaacctc agagtggcgg tggcaagtgc tttaactgtt atcctgctgg 21540 tgttaatatt acactggcca attttaatga aactaaaggg cctttgtgtg ttgacacatc 21600 acacttcact accaaatacg ttgctgttta tgccaatgtt ggtaggtgga gtgctagtat 21660 taacacggga aattgccctt tttcttttgg caaagttaat aactttgtta aatttggcag 21720 tgtatgtttt tcgctaaagg atatacccgg tggttgcgca atgcctatag tggctaattg 21780 ggcttatagt aagtactata ctataggctc attgtatgtt tcttggagtg atggtgatgg 21840 aattactggc gtcccacaac ctgttgaggg tgttagttcc tttatgaatg ttacattgga 21900 caaatgtact aaatataata tttatgatgt atctggtgtg ggtgttattc gcgttagcaa 21960 tgacaccttt cttaatggaa ttacgtacac atcaacttca ggtaaccttc tgggttttaa 22020 agatgttact aagggcacca tctactctat cactccttgt aacccaccag atcagcttgt 22080 tgtttatcag caagctgttg ttggtgctat gttgtctgaa aattttacta gttacggctt 22140 ttctaatgtt gtagaactgc cgaaattttt ctatgcgtcc aatggcactt ataattgcac 22200 agacgctgtt ttaacttatt ctagttttgg cgtttgtgca gatggttcta taattgctgt 22260 tcaaccacgt aatgtttcat atgatagtgt ttcagctatc gtcacagcta atttgtctat 22320 accttccaat tggaccactt cggtccaggt tgagtattta caaattacaa gtacacctat 22380 cgtagttgat tgctccactt atgtttgcaa tggtaatgtg cgctgtgttg aattgcttaa 22440 gcagtatact tctgcttgta aaaccataga agacgcctta agaaatagcg ccaggctgga 22500 gtctgcagat gttagtgaga tgctcacttt tgacaagaaa gcgtttacac ttgctaatgt 22560 tagtagtttt ggtgactaca accttagcag cgtcatacct agcttgccca caagtggtag 22620 tagagtggct ggtcgcagtg ccatagaaga catacttttt agcaaacttg ttacttctgg 22680 acttggcact gtggacgcag actacaaaaa ttgcactaag ggtctttcca ttgctgactt 22740 ggcttgtgct caatattata atggcattat ggttttgcct ggcgtcgctg atgctgaacg 22800 aatggccatg tatacaggtt ctttaattgg tggaattgct ttaggaggtc taacatcagc 22860 cgtttcaata ccattttcat tagcaattca ggcacgttta aattatgttg cattgcagac 22920 tgatgtttta caagaaaatc agaaaattct tgctgcatct tttaacaaag caatgaccaa 22980 catagtagat gcctttactg gtgttaatga tgctattaca caaacttcac aagccctaca 23040 aacagttgct actgcactta acaagatcca ggatgttgtt aatcaacaag gcaacttatt 23100 gaaccattta acttctcagt tgaggcagaa ttttcaagct atctctagct ctattcaggc 23160 tatctatgac agacttgaca ctattcaggc tgatcaacaa gtagataggc tgattactgg 23220 tagattggct gctttgaatg tattcgtttc tcatacattg actaagtaca ctgaagttcg 23280 tgcttccaga cagcttgcac aacaaaaagt gaatgagtgt gtcaaatccc agtctaagcg 23340 ttatggcttc tgtggaaatg gcactcacat tttctcaatt gttaatgctg ctcctgaggg 23400 gcttgttttt ctccacactg tcttgttgcc gacacaatat aaggatgttg aagcgtggtc 23460 tgggttgtgc gttgatggta caaacggtta tgtgttgcga caacctaatc ttgctcttta 23520 caaagaaggc aattattata gaatcacatc tcgcataatg tttgaaccac gtattcctac 23580 catggcagat tttgttcaaa ttgaaaattg caatgtcaca tttgttaaca tttctcgctc 23640 tgagttgcaa accattgtgc cagagtatat tgatgttaat aagacgctgc aagaattaag 23700 ttacaaattg ccaaattaca ctgttccaga cctagttgtc gaacagtaca accagactat 23760 tttgaatttg accagtgaaa ttagcaccct tgaaaataaa tctgcggagc ttaattacac 23820 tgttcaaaaa ttgcaaactc tgattgacaa cataaatagc acattagtcg acttaaagtg 23880 gctcaaccgg gttgagactt acatcaagtg gccgtggtgg gtgtggttgt gcatttcagt 23940 cgtgctcatc tttgtggtga gtatgttgct attatgttgt tgttctactg gttgctgtgg 24000 cttctttagt tgttttgcat cttctattag aggttgttgt gaatcaacta aacttcctta 24060 ttacgacgtt gaaaagatcc acatacagta atggctctag gtttgttcac attgcaactt 24120 gtgtctgctg ttaatcaatc gcttagcaat gcgaaagtta gtgctgaagt ttcacgacag 24180 gttatccaag acgtgaaaga tggcactgtt accttcaact tgctagcgta tacactaatg 24240 agcctctttg ttgtgtattt tgctttattt aaagcaagat cacaccgtgg cagagctgct 24300 cttatagtgt ttaaaattct aatccttttc gtttatgtgc cattgctgta ttggtctcaa 24360 gcatatattg acgcaacttt gattgctgta attttgcttg gaagattttt ccatacagct 24420 tggcactgct ggctctacaa gacatgggat ttcattgtct tcaatgtaac cacactttgc 24480 tatgcaaggt aagtgttggt ttcttgaaaa taaggctctg aaaccattcg tttgttttta 24540 cggaggggat caattccttt acataggcga cagaattgtt tcttatctct caactaacga 24600 cttgtacgtt gctcttagag gacgtattga taaagacctc agcctttcta gaaaggttga 24660 gttatataac agtgaatgtg tatacttgtt ttgtgaacac ccagctgttg gaatagtcaa 24720 cacagatttc aaattagaaa tccactaaga tgttccttaa gctagtggat gatcatgctt 24780 tggttgttaa tgtactactc tggtgtgtgg tgcttatagt gatactacta gtgtgtatta 24840 caataattaa actaactaag ctttgtttca cttgccatat gttttgtaat agaacagttt 24900 atggccccat taaaaatgtg taccacattt accaatcata tatgcacata gaccctttcc 24960 ctaaacgagt tattgatttc taaactaaac gacaatgtca aatgacaatt gtacgggtga 25020 cattgtcacc catttgaaga agtggaattt tggttggaat gttattctaa ccatattcat 25080 tgttattctt cagtttggac actataaata ctccagattg ttttatggtt tgaagatgct 25140 tgtactgtgg cttctttggc cactcgtact tgctttgtca atctttgaca cctgggctaa 25200 ttgggattct aattgggcct ttgttgcatt tagctttctt atggccgtat caacactcgt 25260 tatgtgggtg atgtacttcg caaacagttt cagacttttc cgacgtgctc gaactttttg 25320 ggcatggaat cctgaggtca atgcaatcac tgtcacaacc gtgttgggac agacatacta 25380 tcaacccatt caacaagctc caacaggcat tactgtgacc ttgctgagcg gcgtgcttta 25440 cgttgacgga catagattgg cttcaggtgt tcaggttcat aacctacctg aatacatgac 25500 agttgccgtg ccgagcacta ctataattta tagtagagtc ggaaggtccg taaattcaca 25560 aaatagcaca ggctgggttt tctacgtacg agtaaaacac ggtgattttt ctgcagtgag 25620 ctctcccatg agcaacatga cagaaaacga aagattgctt catttttcct aaactgaacg 25680 aaaagatggc tacagtcaaa tgggctgatg catctgaacc acaacgtggt cgtcagggta 25740 gaatacctta ttctctttat agccctttgc ttgttgatag tgaacaacct tggaaggtga 25800 tacctcgtaa tttggtaccc atcaacaaga aagacaaaaa taagcttata ggctattgga 25860 atgttcaaaa acgtttcaga actagaaagg gcaaacgggt ggatttgtca cccaagctgc 25920 atttttatta tcttggcaca ggaccccata aagatgcaaa atttagagag cgtgttgaag 25980 gtgtcgtctg ggttgctgtt gatggtgcta aaactgaacc tacaggttac ggtgttaggc 26040 gcaagaattc agaaccagag ataccacact tcaatcaaaa gctcccaaat ggtgttactg 26100 ttgttgaaga acctgactcc cgtgctcctt cccggtctca gtcgaggtcg cagagtcgcg 26160 gtcgtggtga atccaaacct caatctcgga atccttcaag tgacagaaac cataacagtc 26220 aggatgacat catgaaggca gttgctgcgg ctcttaaatc tttaggtttt gacaagcctc 26280 aggaaaaaga taaaaagtca gcgaaaacgg gtactcctaa gccttctcgt aatcagagtc 26340 ctgcttcttc tcaaacttct gccaagagtc ttgctcgttc tcagagttct gaaacaaaag 26400 aacaaaagca tgaaatgcaa aagccacggt ggaaaagaca gcctaatgat gatgtgacat 26460 ctaatgtcac acaatgtttt ggccccagag accttgacca caactttgga agtgcaggtg 26520 ttgtggccaa tggtgttaaa gctaaaggct atccacaatt tgctgagctt gtgccgtcaa 26580 cagctgctat gctgtttgat agtcacattg tttccaaaga gtcaggcaac actgtggtct 26640 tgactttcac tactagagtg actgtgccca aagaccatcc acacttgggt aagtttcttg 26700 aggagttaaa tgcattcact agagaaatgc aacaacatcc tcttcttaac cctagtgcac 26760 tagaattcaa cccatctcaa acttcacctg caactgctga accagtgcgt gatgaagttt 26820 ctattgaaac tgacataatt gatgaagtaa actaaacatg ccactgtgtt gtttgaaatt 26880 caggctttag ttggaatttt gcttttgttc ttgcttttat tatctttctt ttgcctgttt 26940 ttagagagat ttggcgcctt ggtgccgtag atgaatacat tgcttttctc tgatctatgt 27000 atgatggtac gatcagagct gcttttaatt aacatgatcc cttgctttgg cttgacaagg 27060 atctagtctt atacacaatg gtaagccagt ggtagtaaag gtataagaaa tttgctacta 27120 tgttactgaa cctaggtgaa cgctagtata actcattaca aatgtgctgg agtaatcaaa 27180 gatcgcattg acgagccaac aatggaagag ccagtcattt gtcttgagac ctatctagtt 27240 agtaactgct aatggaacgg tttcgatatg gatacacaaa aaaaaaaaaa aaaaaaaaaa 27300 aaaaaaaaaa aaaaaaa 27317

Claims (19)

하기의 단계를 포함하는 분리된 시료 내 알파 코로나 바이러스의 전장 유전체 서열 확인 방법
(a) 알파 코로나 바이러스 전장 유전체에 상보적인 하나 이상의 정방향 및 역방향 프라이머 세트를 제조하는 단계;
(b) 시료에서 RNA를 분리하는 단계;
(c) 분리된 RNA를 사용하여 역전사 반응을 수행하는 단계;
(d) 역전사 반응이 수행된 시료에 하나 이상의 프라이머 세트를 첨가하여 중합효소 연쇄반응을 실시하는 단계; 및
(e) 증폭된 산물의 서열을 분석하는 단계Method for identifying the full-length genomic sequence of alpha coronavirus in an isolated sample comprising the following steps:
(a) preparing one or more forward and reverse primer sets complementary to the alpha corona virus full length genome;
(b) isolating RNA from the sample;
(c) performing a reverse transcription reaction using the isolated RNA;
(d) performing a polymerase chain reaction by adding one or more primer sets to the sample subjected to the reverse transcription reaction; And
(e) analyzing the sequence of the amplified product 제1항에 있어서, 상기 프라이머 세트는 서열번호 1 내지 서열번호 20으로 이루어진 군에서 선택된 프라이머 세트를 포함하는 것인, 방법The method of claim 1, wherein the primer set comprises a primer set selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 20. 제1항에 있어서, 상기 프라이머 세트는 서열번호 1 내지 서열번호 20으로 이루어진 10개의 프라이머 세트를 포함하는 것인, 방법The method of claim 1, wherein the primer set comprises 10 primer sets consisting of SEQ ID NO: 1 to SEQ ID NO: 20. 제1항에 있어서, 상기 시료는 혈액, 혈청, 객담, 소변 또는 생체 조직으로부터 분리된 것인, 방법 The method of claim 1, wherein the sample is isolated from blood, serum, sputum, urine or biological tissue. 제1항에 있어서, 상기 알파 코로나 바이러스는 229E 또는 NL63을 포함하는 것인, 방법The method of claim 1, wherein the alpha corona virus comprises 229E or NL63. 제1항에 있어서, 상기 (e) 단계는 서열번호 31 내지 87의 프라이머를 사용하는 것인, 방법The method according to claim 1, wherein the step (e) uses primers of SEQ ID NOs: 31 to 87. 알파 코로나 바이러스 전장 유전체에 상보적인 하나 이상의 정방향 및 역방향 프라이머 세트를 포함하는 알파 코로나 바이러스 진단 키트Alpha Corona Virus Diagnostic Kit containing one or more sets of forward and reverse primers complementary to the alpha corona virus full-length genome. 제7항에 있어서, 상기 프라이머 세트는 서열번호 1 내지 서열번호 20으로 이루어진 군에서 선택된 프라이머 세트를 포함하는 것인, 키트The kit of claim 7, wherein the primer set comprises a primer set selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 20. 제7항에 있어서, 상기 프라이머 세트는 서열번호 1 내지 서열번호 20으로 이루어진 10개의 프라이머 세트를 포함하는 것인, 키트The kit of claim 7, wherein the primer set comprises 10 primer sets consisting of SEQ ID NO: 1 to SEQ ID NO: 20. 제7항에 있어서, 진단 시료는 혈액, 혈청, 객담, 소변 또는 생체 조직으로부터 분리된 것인, 키트The kit of claim 7, wherein the diagnostic sample is isolated from blood, serum, sputum, urine or biological tissue. 제7항에 있어서, 상기 알파 코로나 바이러스는 229E 또는 NL63을 포함하는 것인, 키트The kit of claim 7, wherein the alpha corona virus comprises 229E or NL63. 제7항에 있어서, 알파 코로나 바이러스의 서열 분석은 서열번호 31 내지 87의 프라이머를 사용하는 것인, 키트The kit of claim 7, wherein the sequence analysis of the alpha corona virus uses primers of SEQ ID NOs: 31 to 87. 알파 코로나 바이러스 전장 유전체에 상보적인 하나 이상의 정방향 및 역방향 프라이머 세트를 포함하는 알파 코로나 바이러스 유발 질환 진단용 조성물Compositions for diagnosing alpha corona virus-induced diseases comprising one or more sets of forward and reverse primers complementary to the alpha corona virus full-length genome 제13항에 있어서, 상기 코로나 바이러스 유발 질환은 호흡기 질환인 것인, 조성물The composition of claim 13, wherein the coronavirus-induced disease is a respiratory disease. 제13항에 있어서, 상기 프라이머 세트는 서열번호 1 내지 서열번호 20으로 이루어진 군에서 선택된 프라이머 세트를 포함하는 것인, 조성물The composition of claim 13, wherein the primer set comprises a primer set selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 20. 제13항에 있어서, 상기 프라이머 세트는 서열번호 1 내지 서열번호 20으로 이루어진 10개의 프라이머 세트를 포함하는 것인, 조성물The composition of claim 13, wherein the primer set comprises 10 primer sets consisting of SEQ ID NO: 1 to SEQ ID NO: 20. 제13항에 있어서, 진단 시료는 혈액, 혈청, 객담, 소변 또는 생체 조직으로부터 분리된 것인, 조성물The composition of claim 13, wherein the diagnostic sample is isolated from blood, serum, sputum, urine or biological tissue. 제13항에 있어서, 상기 알파 코로나 바이러스는 229E 또는 NL63을 포함하는 것인, 조성물The composition of claim 13, wherein the alpha corona virus comprises 229E or NL63. 제13항에 있어서, 알파 코로나 바이러스의 서열 분석은 서열번호 31 내지 87의 프라이머를 사용하는 것인, 조성물

The composition of claim 13, wherein the sequencing of the alpha corona virus uses the primers of SEQ ID NOs: 31-87.

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