KR20200051997A - Cosmetic Composition For Improving Wrinkle Containing Retinol, Glutathione Or Tocopherol Stabilized By Nano-Structured Lipid Carrier - Google Patents

Abstract

The present invention relates to a cosmetic composition for ameliorating skin wrinkles, containing a nanostructured lipid carrier composed of retinol, glutathione and tocopherol as an active ingredient. The nanostructured lipid carrier ensures enhanced stability due to solid lipid and liquid lipid in which retinol, glutathione and tocopherol contained as the active ingredient from a matrix of the carrier. Thus, the cosmetic composition for ameliorating skin wrinkles containing the same in an amount of 0.001 to 1 wt% has remarkably high wrinkle amelioration effects.

Description

Cosmetic Composition For Improving Wrinkle Containing Retinol, Glutathione Or Tocopherol Stabilized By Nano-Structured Lipid Carrier} Containing Retinol, Glutathione or Tocopherol Stabilized with Nanostructured Lipid Transporter

The present invention is a cosmetic composition for improving skin wrinkles, which stabilizes an active ingredient with a nanostructured lipid transporter, more specifically, enhances stabilization of an active ingredient with a nanostructured lipid transporter, and improves wrinkles by mixing retinol, glutathione and tocopherol. It is characterized by excellent.

In skin care, the demand for skin aging relief is steadily high. Wrinkles and pigmentation are some of the signs of skin aging, but the biggest is wrinkles. Efficacy ingredients for relieving skin aging include retinol, glutathione, and tocopherol.

However, since these efficacy substances have low storage stability, it is very difficult to apply them to cosmetic compositions. Due to this relationship, many studies have been conducted on stabilization of retinol. So far, looking at the stabilization of retinol and its derivatives, it can be seen that stabilization is achieved by changing the type of water-soluble antioxidant and chelate depending on the form. However, since they cannot completely protect unstable retinol from oxygen, moisture, and heat, effective stabilization of retinol for a long time is difficult.

In addition, U.S. Patent No. 5,855,826 describes primary stabilization by enclosing retinol in a capsule made of glycosaminoglycan such as collagen or sodium chondroitin sulfate, and matrixing the first stabilized capsule with gellan gum. Although secondary stabilization technology is disclosed, secondary stabilization by gellan gum is an open matrix system, making it difficult to effectively and effectively prevent retinol, which is very sensitive to oxygen, moisture, and heat from contact with oxygen, moisture, and heat. There is a limit. Republic of Korea Patent No. 10-1777616 discloses a method of applying NLC to increase the storage stability of tocopherol, Republic of Korea Patent No. 10-1828838 discloses the storage stability of palm oil phytonutrient (phytonutrient) showing an antioxidant effect Disclosed is a method of applying NLC to enhance.

The present invention has never been discussed with respect to a cosmetic composition having both storage stability and effect enhancement of retinol, glutathione and tocopherol, and the present invention seeks to solve this.

Accordingly, an object of the present invention is to increase the storage stability of retinol, glutathione and tocopherol, which are active ingredients, and to invent a cosmetic composition having an excellent skin wrinkle improvement effect.

In order to solve the above problems, the present inventor provides a cosmetic composition for improving skin wrinkles containing a nanostructured lipid transporter comprising at least one active ingredient selected from the group consisting of retinol, glutathione and tocopherol.

Preferably, the nanostructured lipid transporter is characterized in that the cosmetic composition for improving wrinkles having a range of 0.001 to 1% by weight relative to the total weight of the cosmetic composition.

Preferably, the nanostructured lipid carrier is a cosmetic composition for improving skin wrinkles containing 60 to 95% by weight of a water component, 1 to 10% by weight of a lipid component, and 0.001 to 30% by weight of an active ingredient relative to the total weight of the nanostructured lipid carrier. It is characterized by being.

Preferably, the nanostructured lipid transporter comprises 0.001 to 10% by weight of retinol, 0.001 to 10% by weight of glutathione, and 0.001 to 10% by weight of tocopherol as active ingredients relative to the total weight of the nanostructured lipid transporter. It is characterized in that it is a cosmetic composition for wrinkle improvement.

Preferably, the active ingredient is characterized in that it comprises a retinol, glutathione and tocopherol in a weight ratio of 1 to 3: 1 to 3: 1 to 3, cosmetic composition for improving wrinkles.

Preferably, the cosmetic composition for improving wrinkles is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray It is characterized in that it is a cosmetic composition for improving skin wrinkles having any one formulation selected from the group consisting of.

The cosmetic composition for improving wrinkles prepared according to the manufacturing method of the present invention exhibits an improved wrinkle improvement effect.

Figure 1 shows the average change in the wrinkle roughness of the nanostructured lipid transporter according to the type of active ingredient.
Figure 2 shows the average change in the wrinkle roughness of the nanostructured lipid transporter according to the content of the active ingredient.
Figure 3 shows the average change in wrinkle roughness of the nanostructured lipid transporter according to the active ingredient composition ratio.
Figure 4 shows the average change in the wrinkle roughness of the cosmetic composition for improving wrinkles according to the content of the nanostructured lipid transporter.

All technical terms used in the present invention, unless defined otherwise, have the following definitions and conform to the meaning as commonly understood by those skilled in the art in the relevant field of the present invention. Also, a preferred method or sample is described herein, but similar or equivalent ones are included in the scope of the present invention.

The term "about" refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, for reference amount, level, value, number, frequency, percent, dimension, size, amount, weight or length It means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length that varies by 4, 3, 2 or 1%.

Throughout this specification, the terms “comprises” and “comprising”, unless the context requires otherwise, include the steps or components presented, or groups of steps or components, but any other steps or components, or It should be understood that it implies that a group of steps or components is not excluded.

In the present invention, a carrier applied to stabilize retinol is nano-structured lipid carriers (NLC). In the case of NLC, both solid and liquid lipids are present in the matrix, leading to an increase in the encapsulating ability of oil-soluble active ingredients [Muller, R. H. et al. Adv. Drug Deliv. Rev. 54 (Suppl. 1): S131-S155 (2002)].

The present invention relates to a cosmetic composition for improving skin wrinkles containing retinol, glutathione and tocopherol stabilized with a nanostructured lipid transporter.

The nanostructured lipid delivery system of the present invention is contained in an amount of 0.001 to 10% by weight in a cosmetic composition for improving skin wrinkles. Preferably, it is contained in 0.001 to 1% by weight.

The nanostructured lipid delivery system of the present invention is characterized in that it contains 60 to 95% by weight of an aqueous component containing water, 1 to 10% by weight of an oily component containing lipid, and 0.001 to 30% by weight of an active ingredient.

The water phase components are purified water, polysorbate 80, glycerin, 1,3-butylene glycol, propanediol, methylpropanediol, dipropylene glycol, propylene glycol, 1,2-hexanediol, EDTA-2Na, Sodium chloride, betaine, trehalose, PEG-32, PEG-75, polyquaternium-51, sodium hyaluronate, hyaluronic acid, xylitol, sodium PCA, erythritol, fucose, It may include one or more selected from the group consisting of glucose, raffinose, maltose, mannitol, allantoin, and carbomer.

The lipid component is caprylic / capric triglyceride, cetyl palmitate, behenyl alcohol, sphingomyelin, lecithin, eleic acid, glycerol distearate, isostearyl alcohol, C _12-13 alkyl lactate, C _12-15 alkyl benzoate, glycol palmitate, neopentyl glycol diethylhexanoate, neopentyl glycol dicaprylate / dicaprate, di-C _12-13 alkyl maleate, diiso Selected from the group consisting of stearyl malate, diethoxyethyl succinate, dicaprylyl ether, dicaprylyl carbonate, dipentaerythrylhexaC _5-9 acid ester, neoheptanoic acid and tridecyl acid It may contain one or more. More preferably, it includes two types of caprylic / capric triglyceride and cetyl palmitate.

The active ingredient may include at least one substance selected from the group consisting of retinol, glutathione and tocopherol. Retinol may be included in the range of 0.001 to 10% by weight, glutathione in the range of 0.001 to 10% by weight, and tocopherol in the range of 0.001 to 10% by weight relative to the total weight of the nanostructured lipid transporter.

The total content of retinol, glutathione and tocopherol as active ingredients in the nanostructured lipid transporter may be included in an amount of 0.001 to 30% by weight. Preferably, the active ingredient may be included in 0.01 to 5% by weight. The mixing ratio of the active ingredient is that retinol, glutathione and tocopherol are 1 to 3: 1 to 3: 1 to 3, and more preferably 1: 1: 1.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, etc. may be used as a carrier component. Can be.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspensions such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used.

According to one embodiment of the present invention, the cosmetic composition of the present invention may further include an active ingredient exhibiting effects such as natural product extraction components, whitening, wrinkle improvement, etc., in order to provide the functionality of the cosmetic, water phase or oil phase.

Hereinafter, the present invention will be described in more detail through examples. These examples are only intended to illustrate the present invention more specifically, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

[Example 1]: Preparation of nanostructured lipid transporter for stabilization of active ingredient

Nanostructured lipid transporter for liquid lipid and solid lipid composition ratios Prize ingredient Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Preparation Example 5 Preparation Example 6 Water phase ingredients Purified water 87.50 87.50 87.50 87.50 87.50 87.50 Polysorbate 80 2.50 2.50 2.50 2.50 2.50 2.50 Lipid composition Caprylic / Capric Triglyceride 5.00 4.00 3.00 2.00 1.00 0.00 Cetyl palmitate 0.00 1.00 2.00 3.00 4.00 5.00 Active ingredient Retinol 5.00 5.00 5.00 5.00 5.00 5.00 total 100.00 100.00 100.00 100.00 100.00 100.00

The production methods of Preparation Examples 1 to 6 are all the same, and the liquid lipid and solid lipid composition ratios are different. First, the nanostructured lipid transporter dissolved the oil phase at a temperature higher than the melting point of the oil phase to prevent recrystallization of lipid components. In this example, dissolution was performed at 75 ° C. The aqueous phase component was heated to 75 ° C., which is the same temperature as the lipid component, for easy stirring when the lipid component was added. The dissolved lipid component is stirred with retinol as an active ingredient. Subsequently, (lipid component + active component) is added to the water phase component and emulsified for 5 minutes at 3000 rpm with a homogenizer. After the emulsification is finished, it is circulated 3 times at a pressure of 800 bar at 75 ° C. through a microflow die and cooled to below 30 ° C.

[Example 2]: Preparation of nanostructured lipid transporter: selection of active ingredient type

Nanostructured lipid transporter for active ingredient types Prize ingredient Preparation Example 2 Preparation Example 7 Preparation Example 8 Preparation Example 9 Preparation Example 10 Water phase ingredients Purified water 89.50 89.50 89.50 89.50 89.50 Polysorbate 80 2.50 2.50 2.50 2.50 2.50 Lipid composition Caprylic / Capric Triglyceride 4.00 4.00 4.00 4.00 4.00 Cetyl palmitate 1.00 1.00 1.00 1.00 1.00 Active ingredient Retinol 3.00 1.00 Glutathione 3.00 1.00 Tocopherol 3.00 1.00 Palm You Phyto Nutrition 3.00 total 100.00 100.00 100.00 100.00 100.00

Example 2 was made in the same process as in Example 1, the type and composition of the active ingredient was different. The palm oil phytonutrient of Preparation Example 9 was used to compare efficacy and effectiveness. The sample is a concentrated liquid obtained by reacting natural palm oil with alcohol according to a patent related to the NLC manufacturing method using the palm oil phytonutrient, which is an antioxidant (Patent Registration No. 10-1828838-0000), to form an alkyl ester and glycerol, and vacuum distilling the alkyl ester. Was applied.

[Example 3]: Preparation of nanostructured lipid transporter: Determination of active ingredient content range

Nanostructured lipid transporter according to the content of active ingredients Prize ingredient Preparation Example 11 Preparation Example 12 Preparation Example 13 Preparation Example 14 Preparation 15 Water phase ingredients Purified water 92.50 92.497 77.50 62.50 47.50 Polysorbate 80 2.50 2.50 2.50 2.50 2.50 Lipid composition Caprylic / Capric Triglyceride 4.00 4.00 4.00 4.00 4.00 Cetyl palmitate 1.00 1.00 1.00 1.00 1.00 Active ingredient Retinol 0.001 5.00 10.00 15.00 Glutathione 0.001 5.00 10.00 15.00 Tocopherol 0.001 5.00 10.00 15.00 total 100.00 100.00 100.00 100.00 100.00

Example 3 was made in the same process as in Example 1, the content of the active ingredient was different.

[Example 4]: Preparation of nanostructured lipid transporter: confirming the effect of the active ingredient composition ratio

Nanostructured lipid carrier according to active ingredient composition ratio Prize ingredient Preparation 16 Preparation Example 17 Preparation Example 18 Water phase ingredients Purified water 89.50 89.50 89.50 Polysorbate 80 2.50 2.50 2.50 Lipid composition Caprylic / Capric Triglyceride 4.00 4.00 4.00 Cetyl palmitate 1.00 1.00 1.00 Active ingredient Retinol 0.50 1.25 1.25 Glutathione 1.25 0.50 1.25 Tocopherol 1.25 1.25 0.50 total 100.00 100.00 100.00

Example 4 was made in the same process as in Example 1, the composition ratio of the active ingredient was different.

[Example 5]: Preparation of cosmetic composition for improving skin wrinkles on the content of nanostructured lipid transporter

Prize ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Phase A Purified water 79.898 79.897 79.888 79.798 79.898 Disodium ID 0.02 0.02 0.02 0.02 0.02 glycerin 2.8 2.8 2.8 2.8 2.8 1,2-hexanediol 2 2 2 2 2 Phase B Polyglyceryl-3 methyl glucose distearate 1.6 1.6 1.6 1.6 1.6 PAGE-4 Olive 0.4 0.4 0.4 0.4 0.4 Cetearyl alcohol 0.2 0.2 0.2 0.2 0.2 Squalane 4.5 4.5 4.5 4.5 4.5 Caprylic / Capric Triglyceride 4.2 4.2 4.2 4.2 4.2 Dimethicone 1.3 1.3 1.3 1.3 1.3 Phase C Preparation Example 12 0.001 0.01 0.1 One D phase Purified water 2.94 2.94 2.94 2.94 2.94 Acrylate / C10-30 alkyl acrylate cross polymer 0.06 0.06 0.06 0.06 0.06 E phase Purified water 0.0738 0.0738 0.0738 0.0738 0.0738 Potassium hydroxide 0.0082 0.0082 0.0082 0.0082 0.0082 total 100 100 100 100 100

The method of implementing the cosmetic composition for improving skin wrinkles with respect to the content of the nanostructured lipid transporter was the same in all of the formulation examples 1 to 5, and the content of the nanostructured lipid transporter (Production Example 12) was different. First, the A-phase and B-phase were heated to 75 ° C, then stirred at 3000 rpm in a homogenizer for 5 minutes, cooled to 45 ° C, and the C, D, and E phases were added for 3 minutes each, followed by mixing at 2800 rpm. , Cool to 30 ℃.

[Test Example 1] Nanostructured lipid transporter stability evaluation: stability of separation and discoloration

Preparation Examples 1 to 6 were stored in an opaque vial container in a constant temperature bath maintained at a constant temperature of 45 ° C. and stored for 12 weeks to compare and measure the degree of separation and discoloration, and the results are shown in Table 6 below. At this time, the degree of product separation and discoloration was evaluated by classifying the following six grades.

Product discoloration evaluation criteria:

0: No change, 1: Very little separation (discoloration), 2: Little separation (discoloration), 3: Little separation (discoloration), 4: Bad separation (discoloration) 5: Extremely severe separation (discoloration)

Separation and discoloration stability for nanostructured lipid transporters by lipid composition Elapsed time Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Preparation Example 5 Preparation Example 6 2 0.0 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 0.0 8 0.5 0.0 0.0 0.0 0.5 0.5 10 1.0 0.0 0.5 0.5 0.5 1.0 12 1.5 0.5 0.5 0.5 1.0 1.5

As a result of the test, in Preparation Example 2, even after 12 weeks, there was only a slight discoloration that was difficult to distinguish with the naked eye, whereas Preparation Example 1 prescribed with only liquid lipids began to show slight discoloration after 8 weeks, and after 12 weeks The visible discoloration was visible, and in the case of Preparation Examples 3 and 4, some discoloration occurred after 10 weeks, and Preparation Examples 5 and 6 began to show some discoloration after 8 weeks, and 12 weeks elapsed. Poems showed discoloration phenomena that were discernible with the naked eye.

Looking at this, when the composition of the liquid lipid caprylic / capric triglyceride and the solid lipid cetyl palmitate is 4: 1, it can be seen that the nanostructured lipid transporter composition is stable in separation and discoloration.

These results result in discoloration due to loss of stability due to the release of retinol captured during the storage period when the liquid lipid content is excessive, and when the solid lipid content is excessive, the retinol is not captured by the nanostructured lipid transporter. It is believed that this is due to discoloration due to the lack of stability.

[Test Example 2] Nanostructured lipid transporter stability evaluation: active ingredient storage stability

After the composition of Preparation Examples 1 to 6 of Example 1 was stored at 45 ° C. for 12 weeks, the retinol content in each content was analyzed by high-speed liquid chromatography. Each content was diluted to a concentration of 20 mg / mL in a mixed solution of methanol and ethyl acetate (50 weight each), then eluted with tetrahydrofuran / acetonitrile (5:95) and 0.05N ammonium acetate solution. A reversed phase column (C-8) was used, and the content of retinol was analyzed at 340 nm. Based on the content of retinol immediately after preparation of each content as 100, the retinol content after storage at 45 ° C. for 12 weeks was calculated. The test results are shown in [Table 7] below.

Active component titer stability for nanostructured lipid transporters by lipid composition Elapsed time Preparation Example 1 Preparation Example 2 Preparation Example 3 Preparation Example 4 Preparation Example 5 Preparation Example 6 2 99.0 99.0 99.0 99.0 99.0 99.0 4 99.0 99.0 99.0 99.0 99.0 99.0 6 98.5 99.0 99.0 99.0 98.5 98.5 8 98.0 99.0 98.5 98.5 98.5 98.0 10 97.5 99.0 98.0 98.0 98.0 97.5 12 97.0 98.5 97.5 97.5 97.5 97.0

As a result of the test, the retinol content of Preparation Example 2 was almost unchanged for 10 weeks and slightly decreased after 12 weeks, whereas Preparation Examples 1 and 3 to 6 began to drop slightly after 6 to 8 weeks, and after 12 weeks, a considerable amount of content You can see that it is very unstable. From this, it can be seen that when the composition of the liquid lipid and solid lipid is 4: 1, the titer of retinol is very effectively preserved.

[Test Example 3] Skin wrinkle improvement effect: nano-structured lipid delivery system for active ingredient types

Females over 30 years old (average age 37.5 years old) are paired in groups of 10, and each formulation is applied to the area of 2X2 cm 2 of the upper arm of the arm (2 times / day, 0.2 g / time) for 12 weeks, and then made of a transparent silicone material. Using a solution, the replica of the skin wrinkles was floated, and the skin wrinkle change was measured with a skin wrinkle meter (SKIN VISIOMETER SV400, C + K Electronics GmbH. Germany). Three-dimensional analysis of the image of the replica with a CCD camera, and the effect of wrinkle improvement is analyzed by the average wrinkle roughness (Rz) of the following formula, which is the sum of the roughness of each wrinkle (Rm: m is an integer greater than or equal to 1) divided by the number of wrinkles. Did. The test results are shown in the following [Fig. 1], the average of the wrinkle roughness change value of each group 10.

As a result of the test for the nanostructured lipid transporter for the active ingredient type, it was confirmed that the preparation example 10 was lowered by 25.0 µm (p <0.01), and the wrinkles were significantly improved compared to the preparation examples 2, 7, 8, and 9.

Therefore, it can be seen that the mixture of retinol, glutathione, and tocopherol exhibits a synergistic effect on the improvement of skin wrinkles compared to the use of each. In addition, it can be seen that it is very effective to apply a mixture of retinol, glutathione, and tocopherol compared to palm oil phytonutrient.

[Test Example 4] Skin wrinkle improvement effect: nano-structured lipid carrier according to the content of active ingredients

The test method was the same as in Test Example 3, and the effect of improving skin wrinkles on the nanostructured lipid carrier according to the content of the active ingredient was evaluated. The test results are shown in the following [Fig. 2], the average for the change in wrinkle roughness of each group of 10 people.

When the content of the active ingredient increased, the skin wrinkle roughness change value tended to increase, and the total content of the active ingredient increased significantly up to 30%, but when the skin wrinkle roughness change value of 30% to 45% was compared. It can be seen that the width is relatively small.

Therefore, when the content of retinol, glutathione, and tocopherol in the nanostructured lipid transporter is contained in an amount of 0.001% or more, skin wrinkle improvement is effective, and when each content exceeds 10%, the effect is increased, but the efficiency compared to the content is significantly lowered. Confirmed.

[Test Example 5] Skin safety test: nanostructured lipid delivery system according to the content of active ingredients

The subjects (average age 25.9 years, age distribution 18-33 years) were paired into groups of 10 people, and the formulation that passed about 2 months at room temperature was subjected to a patch test on the upper arm using Haye's Test Chamber. Did. However, those with psoriasis, eczema, and other skin lesions or those who were pregnant, lactating or contraceptives, or antihistamines were excluded from the study.

After the test site is wiped with 70% ethanol and dried, 15 μg of each formulation is dropped into the chamber, and then fixed by placing it on the upper part of the test site. The patch was applied for 24 hours and after removing the patch, the test site was marked with a marking pen to observe the test site after 24, 48 and 72 hours, respectively.

The determination was performed 24 hours, 48 hours, and 72 hours after removal of the patch, and the skin reaction was determined according to the rules of the International Contact Dermatitis Research Group (ICDRG) in Table 8 below. The test results are shown in Table 9 below for the average value of the 10 groups for the formulation.

Criteria for International Contact Dermatitis Research Council sign Criteria evaluation Mean Score - Doubtful of slight reaction and erythema
Erythema + Induration Erythema + Induration + VesicleErythema + Induration + BullaeNegative Irritation 0.0 ± Smile stimulation 0.0-0.9 + Light stimulation 1.0-2.9 ++ Stimulation 3.0-4.9 +++ Strong stimulation 5.0 or higher

Skin irritation: Nanostructured lipid transporter according to active ingredient content Elapsed time (hours) Preparation Example 10 Preparation Example 11 Preparation Example 12 Preparation Example 13 Preparation Example 14 Preparation 15 24 0.0 0.0 0.0 0.0 0.1 1.0 48 0.0 0.0 0.0 0.0 0.0 0.5 72 0.0 0.0 0.0 0.0 0.0 0.2

As a result of the skin safety test, Preparation Examples 11 to 14 showed no irritation on the basis of 72 hours, whereas Preparation Example 15 showed micro-stimulation.

In addition, after 24 hours, Preparation Examples 11 to 13 showed no stimulation, and Production Example 14 showed micro-stimulation, whereas Production Example 15 showed light stimulation.

Therefore, it was confirmed that when the content of each of the retinol, glutathione, and tocopherol in the nanostructured lipid transporter exceeds 10%, skin irritation may be exhibited.

[Test Example 6] Skin wrinkle improvement effect: nanostructured lipid delivery system according to the active ingredient composition ratio

The test method was the same as in Test Example 3, and the effect of improving skin wrinkles on the nanostructured lipid carrier according to the composition ratio of the active ingredient was evaluated. The test results are shown in the following [Fig. 3], the average of the wrinkle roughness change value of each group 10.

Retinol, glutathione, tocopherol (1) 1: 1: 1, (2) 1: 2.5: 2.5, (3) 2.5: 1: 2.5, (4) 2.5: 2.5: Test ratio by ratio It was confirmed that the skin wrinkle improvement rate was similar.

[Test Example 7] Skin wrinkle improvement effect: cosmetic composition for improving skin wrinkles on the content of nanostructured lipid carriers

The test method is the same as in Test Example 3, and the effect of improving skin wrinkles on the cosmetic composition for improving wrinkles on the content of the nanostructured lipid transporter (Production Example 12) was evaluated. The test results are shown in [Fig. 4] below, the average of the wrinkle roughness change value of each group of 10 people.

As a result of the test, it was confirmed that in the case of the cosmetic composition for improving skin wrinkles containing 0.001% or more, the value of the change in the roughness of the skin wrinkles appeared to be effective compared to the addition of the nanostructured lipid carrier.

Claims (6)

A cosmetic composition for improving skin wrinkles containing a nanostructured lipid delivery system comprising at least one active ingredient selected from the group consisting of retinol, glutathione and tocopherol. According to claim 1, The nano-structured lipid carrier is a cosmetic composition for improving skin wrinkles having a range of 0.001 to 1% by weight relative to the total weight of the cosmetic composition. The method of claim 1, wherein the nanostructured lipid transporter is for improving skin wrinkles containing 60 to 95% by weight of the aqueous component, 1 to 10% by weight of the lipid component, and 0.001 to 30% by weight of the active ingredient relative to the total weight of the nanostructured lipid carrier. Cosmetic composition. The method according to claim 1, wherein the nanostructured lipid transporter comprises 0.001 to 10% by weight of retinol, 0.001 to 10% by weight of glutathione, and 0.001 to 10% by weight of tocopherol as an active ingredient relative to the total weight of the nanostructured lipid transporter. A cosmetic composition for improving skin wrinkles. The cosmetic composition for improving skin wrinkles according to claim 1, wherein the active ingredient comprises retinol, glutathione, and tocopherol in a weight ratio of 1-3: 1-3: 1-3. The method of claim 1, wherein the cosmetic composition for improving skin wrinkles is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and A cosmetic composition for improving skin wrinkles having any one formulation selected from the group consisting of sprays.

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