KR20200046768A - New microorganism Enterobacter asburiae ObRS-5 or microbial agent comprising the same - Google Patents

Abstract

The present invention relates to an Enterobacter asburiae obRS-5 (microbial accession number: KACC 81075BP) strain or a microbial agent containing the same. The microbial agent using the strain has no environmental pollution and human toxicity so it is possible to control environment-friendly plague or promote plant growth for plants such as Ligularia fischeri etc., thereby being able to increase the number of crops.

Description

New microorganism Enterobacter asburiae ObRS-5 or microbial agent comprising the same}

The present invention relates to a microorganism microorganism Enterobacter asburiae ObRS-5 ( Enterobacter asburiae ObRS-5, microorganism accession number: KACC 81075BP) strain or a microbial agent containing the same, more specifically, the plant reverse pathogen Phytoptopra dre A novel microbial enterobacter asburier ObRS-5 strain having a control effect against Phytophthora drechsleri or a microbial agent containing the same as an active ingredient.

Gomchi ( Ligularia fischeri ) is a ripe wild vegetable that grows in a forest with high humidity and shade and has been cultivated in facilities or forests in some areas of Gangwon-do, Gyeongsangnam-do, Gyeongsangbuk-do, and Jeollanam-do since the 1990s. Gomchi is one of the wild vegetables that can be eaten raw and has a high taste and aroma, and has a high consumer preference. Gomchi is known as a plant that rarely causes pests while growing in the highlands, but various pests such as late blight, powdery mildew, and white silk disease are cultivated when wheat is cultivated in a facility house, which has a different environment from the existing habitat, such as humidity, temperature, and illuminance. It has been reported to cause problems.

Among the diseases that occur in bear odor , the disease caused by the Phytophrhoa drechsleri pathogen (Phytopthora root rot) is very difficult to control because it spreads rapidly and dies after one onset, and prevention is important (Kwon et al ., 1999). Since chemical control using synthetic pesticides has problems such as environmental pollution and toxicity to livestock, both production farmers and consumers prefer eco-friendly cultivation due to the nature of Gomchi, a wild vegetable that is ingested raw. However, because there is no biological control means such as microbial agents for eco-friendly control of Gomchi plague, farmers are complaining of the difficulty of eco-cultivation of Gomchi. Because germ seed seeds have a low germination rate, it is difficult to grow them by themselves, and the price of gomch seedlings is higher than other crops. In recent 2 ~ 3 years, some of the farmers who cultivated Gomchi had 24.1 ~ 43.1% of Gomchi blight, which significantly reduced the amount of Gomchi production and replaced it with other crops such as coltsfoot, elm or pepper.

In eco-friendly agriculture, useful microorganisms or antagonistic microorganisms play a large role as an alternative to chemical pesticides. Today, agriculture has been devoted to food production by improving varieties, improving soil fertility, controlling pests and removing weeds, but continued use and abuse of pesticides has caused serious contamination of soils and rivers. In addition, since these pesticides remain in crops, causing problems such as toxicity, environmental pollution, and toxicity to humans and livestock, the use of pesticides is currently being strengthened in the world. As concerns about pesticides and interest in quality of life have grown, biological control using microorganisms or plant extracts as a new alternative has recently become a concern.

Microbial agents refer to products made of bacteria, fungi, viruses, etc. that are effective in controlling plant diseases or pests. In addition to pest control, areas that can be used for agriculture include soil improvement, organic matter decomposition promotion, nutrient / moisture absorption promotion, and growth promotion And weeding (Johansson et al., 2004; Khan et al., 2007; Chandler et al., 2008). Biological fertilizer mechanisms such as phosphate solubilization, nitrogen fixation, and plant growth-promoting hormone production are known as useful microorganisms to help plants grow. In addition, lysis, parasitic action, antagonism through space or nutrient competition, production of antibiotics, Indirectly assists plant growth by methods known as biological control mechanisms, such as induction of disease resistance. Fluorescent Pseudomonas sp. Reported to exhibit relatively high populations in healthy soil and crop roots. , Bacillus sp., Streptomyces sp. The back serves as a medium to promote interaction with plants, and microorganisms inhabiting the crop roots are highly likely to be used to increase crop yield, such as efficient biological control of plant pests in the process of forming a relationship with the host plant. It is expected to increase the agricultural utilization of.

While studying the various physiological activities of these useful or antagonistic microorganisms, the present inventors, the new microorganism Enterobacter asburiae ObRS-5 ( Enterobacter asburiae ObRS-5), causes the plague of plants such as bear odors. The present invention was completed by confirming that the control effect against sally ( Phytophthora drechsleri ) is excellent.

Republic of Korea Registered Patent No. 10-1512653 (Invention name: New microorganism strain and microbial agent for eco-friendly fertilizer, Applicant: Kangwon National University Industry-Academy Cooperation Foundation, Registration Date: April 10, 2015) Republic of Korea Registered Patent No. 10-1820010 (Invention name: Nematode control agent containing HK169 strain and its culture as an active ingredient in a new enterobacter having the nematode effect, Applicant: Korea Research Institute of Chemical Technology, Registration Date: January 2018 12 days)

Chandler, D., G. Davidson, W. Grant, J. Greaves and G. Tatchell. 2008. Microbial biopesticides for integrated crop management: an assessment of environmental and regulatory sustainability. Trends Food Sci. Technol. 19 (5): 275-283. Khan, M. S., A. Zaidi and P. A. Wani. 2007. Role of phosphate-solubilizing microorganisms in sustainable agriculture-a review. Agron. Sustain. Dev. 27 (1): 29-43. Kwon, S. B., H. J. Jee, S. B. Bang, K. K. Lee and C. K. Hong. 1999. Phytophthora root rot of Ligularia fischeri caused by P. drechsleri. Korean Soc. Plant Pathol. 5 (1): 58-60. Johansson, J. F., L. R. Paul and R. D. Finlay. 2004. Microbial interactions in the mycorrhizosphere and their significance for sustainable agriculture. FEMS Microbiol. Ecol. 48 (1): 1-13.

An object of the present invention is to provide a novel microorganism Enterobacter asburiae ObRS-5 ( Enterobacter asburiae ObRS-5, microorganism accession number: KACC 81075BP) strain or a microbial agent containing the same. In more detail, an object of the present invention is a novel microorganism Enterobacter asburier having a control effect against Phytophthora drechsleri , a plant pest, or a microorganism preparation containing the same as an active ingredient To provide.

The present invention relates to a novel microorganism Enterobacter asburiae ObRS-5 having a plant disease control efficacy ( Enterobacter asburiae ObRS-5, microorganism accession number: KACC 81075BP).

The plant disease is characterized by being derived from the late blight Phytophthora drechsleri .

The plant may be selected from the group consisting of gomchi, pepper, tomato, cucumber, lettuce, watermelon, mustard, Angelica, onion, cabbage, green onion, radish, ginseng, strawberry, lettuce and garlic.

The Enterobacter asburier ObRS-5 strain includes a gene of 16s rRNA expressed by the nucleotide sequence of SEQ ID NO: 1.

The present invention provides a microbial agent for controlling plant diseases comprising the Enterobacter asburier ObRS-5 strain or a culture medium thereof.

Accordingly, the present invention relates to a microbial pesticide comprising the microbial agent.

The microbial pesticide may be provided in a formulation selected from the group consisting of seed coating agent, seed soaking agent, soil conditioner, composting agent, foliar spraying agent and irrigation spraying agent.

In addition, the present invention is a step of obtaining a diluted solution by diluting the microbial agent 10 to 1000 times; And, providing a method for controlling plant pathogens comprising; dipping the seed to the control target plant, seed coating, seed coating, foliar spray or irrigation spray.

Hereinafter, the present invention will be described in detail.

The medium applicable to the culture of Enterobacter asburier ObRS-5 strain in the present invention is not particularly limited, preferably, Tryptic Soy Broth (TSB), Tryptic Soy Agar (TSA), Nutrient Broth (NA) and NA ( Nutrient Agar) can be used.

The strain culture solution of the present invention may be cultured at pH 5.0-9.0 and 15-35 ° C. of Enterobacter asburier ObRS-5 under aerobic conditions. More preferably, it is better to culture at pH 6.5 to 7.5 and 25 to 30 ° C under aerobic conditions. At this time, it is preferable that the proper incubation time is 24-48 hours, and the culture method may be selected from either liquid shaking culture or solid culture.

The present invention provides a plant disease control agent comprising the Enterobacter asburier ObRS-5 strain or a culture medium thereof, wherein the strain or culture medium thereof can be appropriately diluted with water or a medium prior to strain culture depending on the intended use. have.

The present invention is also not particularly limited in the form of the microbial agent, but is provided as a microbial pesticide, and more preferably, may be provided in the form of a seed coating agent, a soil conditioner, a compost premature agent, a foliar sprayer, or an irrigation sprayer.

As the microbial pesticide, a seed coating agent, soil conditioner, composting agent, foliar spraying agent or irrigation spraying agent may be used as a carrier or excipient used in conventional plant pesticides. As the carrier or excipient, bentonite, surfactant, talc, zeolite and the like can be preferably used.

In addition, the present invention is a step of obtaining a diluent by diluting the microbial agent 10 to 1000 times (1x10 ^{6 to 8} CFU / ㎖); And, providing a method for controlling plant pathogens comprising; dipping the seed to the control target plant, seed coating, seed coating, foliar spray or irrigation spray. The mixing ratio of the seed immersion and the diluent at the time of seed coating to the seed can be appropriately adjusted as long as the seed is immersed or coated. In addition, the application amount of each diluent can be appropriately adjusted according to soil distribution or plant conditions when spraying the foliar or spraying irrigation.

The plant to be controlled or the plant to promote growth of the microbial agent comprising the strain of the present invention or a culture medium thereof is not particularly limited, but is preferably bear, red pepper, tomato, cucumber, lettuce, watermelon, mustard, Angelica, onion, Chinese cabbage, It can be selected from the group consisting of green onion, radish, ginseng, strawberry, lettuce, and garlic. In addition, various plants vulnerable to late blight can be applied as the plant to be controlled.

The present invention relates to an enterobacter asburiae ObRS-5 (microbial accession number: KACC 81075BP) strain or a microbial agent containing the same, which has a control effect against plant pests, and the environment using the strain Since it has no pollution and no human toxicity, it is possible to control the environment-friendly plague or promote plant growth for plants such as bear odor, thereby increasing the number of crops. As a technology related to plant disease control related to Enterobacter asburier, a new entero isolated from the root zone of a dune plant under water deficiency and salinity stress in Korean Patent Registration No. 10-1512653 and Korean Patent Registration No. 10-1820010, respectively. Bacterium Asburier m-4 and a microbial agent for eco-friendly fertilizers having a plant growth promoting effect containing the same, and a technique for HK169 strain of Enterobacter genus having high killing power against tomato root knot nematodes, is disclosed, It can be said to be a different technology from the present invention due to different functions and effects generated therefrom.

1 is a graph showing the proportion of strains showing the antibacterial activity identified in the antagonistic microbial separation process of the present invention.
Figure 2 is a schematic diagram showing the seed bioassay of Enterobacter asburier ObRS-5 strain of the present invention.
Figure 3 is a graph and photographs showing the results of seed bioassay of the Enterobacter asburier ObRS-5 strain of the present invention.
4 is a diagram showing the DNA sequence of the 16S rRNA of the Enterobacter asburier ObRS-5 strain of the present invention.
Figure 5 is a phylogenetic tree showing the genetic location of the Enterobacter asburier ObRS-5 strain of the present invention.
Figure 6 is a photograph showing the control effect of Gomcheok blight by seed coating treatment of Enterobacter asburier ObRS-5 strain of the present invention.
Figure 7 is a photograph showing the effect of controlling the antibacterial deodorant by irrigation treatment of Enterobacter asburier ObRS-5 strain of the present invention.

Hereinafter, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to sufficiently convey the spirit of the present invention to those skilled in the art so that the contents introduced herein are thorough and complete.

<Example 1. Isolation of new strains>

Bacteria were isolated from soils and roots of crop cultivation, roots and roots in 14 regions of Korea, including Gongju, Iksan, Miryang, and Sunchang, to isolate microorganisms for controlling Gomchi plague. For the collected soil and mycobacterium soil, put 3 g into 27 ml of sterile water, shake for 30 minutes at 28 ° C at 180 rpm, take 1 ml and dilute it stepwise in 9 ml of sterile water to tryptic soybean agar (TSA; Difco), 1/10 TSA, Nutrient Agar (NA; Difco) medium. The collected roots were drained after washing with water, and 3 ml of sterile water and a sample were put into a sterilized mortar and thoroughly ground, and a suspension in which 24 ml of sterile water was further added was applied to each medium in the same manner as above. After incubation at 28 ° C. for 48 hours, a single colony having a different color and shape was purely separated, put in a 20% (v / v) glycerol aqueous solution, stored at -70 ° C., and used in the experiment.

<Example 2. Antibacterial activity test against Gomchi blight>

Against the plague Phytophthora drechsleri of the strains isolated in Example 1 Antibacterial activity was tested. Separated strains were streaked at a distance of 35 mm from the center of the Potato Dextrose Agar (PDA; Difco) medium and cultured at 25 ° C. for 24 hours, and sterile water was used as a control. After culturing Phytophthora drechsleri in the middle of PDA medium for 5 days at 25 ° C and incubating with the isolates, the size of the inhibition zone was measured to select microorganisms with excellent antibacterial activity.

As a result, as a result of assaying the antagonistic power of Phytophthora drechsleri of 385 isolates as shown in FIG. 1, strains having a mycelial growth inhibition rate of more than 25% of late blight are identified as 11 points, and appear as 3% of all isolates. In addition, the strains with mycelial growth inhibition rate of 10% or more and less than 25% were 52 points, and 14% of all isolates were strained. Strains with less than 10% or no inhibitory effect were 322 points, with 84% of all isolates. It appears that most of the isolated bacteria have no antibacterial activity against Phytophthora drechsleri .

Through these results, 63 isolates with a total mycelial growth rate of 17%, that is, mycelial growth inhibition rate of 10% or more, were selected as antagonistic microorganisms.

<Example 3. Microbial selection through seed bioassay>

A bioassay using seeds was performed on 63 isolates having excellent antibacterial activity against Phytophthora drechsleri selected in Example 2. Pepper seeds have a short germination time, high germination rate, evenness , and similar surface area to bear-bearing seeds, and are highly susceptible to Phytophthora drechsleri in seedlings, making them suitable for improving efficiency and reducing time and cost in performing seed bioassays. The bioassay method using seeds was carried out by modifying the method of Chang et al. (2001) (see FIG. 2).

First, the selected isolates were streaked on TSA medium, incubated at 28 ° C. for 24 hours, and then suspended in 0.1% (w / v) peptone water (Becton Dickinson Biosciences, USA) to give a concentration of 1.0x10 ⁸ cfu / ml. It was adjusted (OD ₆₀₀ = 0.5). The Phytophthora drechsleri strain was incubated at 28 ° C. for 24 hours with a 5 mm diameter microflora in the center of 1.5% (w / v) water agar medium to which 0.02% glucose (w / v) was added. Pepper seeds (Manitta, Nongwoo-bio, Korea) were surface sterilized with 1% NaClO and 70% Ethanol solution to remove the microorganisms on the surface, dentified on filter paper moistened with sterile water, germinated at 25 ° C for 3 days, and then rooted The 2 mm germinated seeds were selected. The selected seeds were immersed in the prepared isolate suspension for 3 hours, and then placed on the edge of the water agar medium in which Phytophthora drechselri was cultured (n = 12). In the control group, pepper seeds immersed in sterile water were incubated, and all treatments were incubated with irradiation at 25 ° C. for 14 hours / day. The incidence (%) was examined 7 and 14 days after treatment.

As described above, as a result of performing a seed bioassay to select a strain having a plague suppression effect on 63 primary isolates, the result of the ObRS-5 strain was confirmed to be the best, and this result is shown in FIG. 3. Shown.

The upper 'A' graph in FIG. 3 is a numerical representation of the growth status of pepper seeds identified in 1.5% (w / v) water agar medium of the lower 'I', and in FIG. 3, A of 'A' and 'I' And D were treated with ObRS-5 strain and Phytophthora drechselri , B and E were treated only with Phytophthora drechselri , and C and F were untreated.

In the results of FIG. 3, the incidence of treatment with only Phytophthora drechselri is 80.6% on day 7 and 94.4% on day 14, but the incidence of late blight on treatments (A and D) treated with ObRS-5 strain and Phytophthora drechselri is 7 days To 11.1% and 30.6% on the 14th, it can be confirmed that there is a remarkable plague suppression effect.

<Example 4. Physiological characteristics of selected strains, 16S rDNA sequence analysis and phylogenetic tree preparation>

In order to identify the selected strain ObRS-5, the culture conditions were checked. The strain grows better in aerobic conditions than in anaerobic conditions, and is confirmed to be cultivable at pH 5.0-9.0 and 15-35 ° C. More preferably, the culture is good at pH 6.5 to 7.5 and 25 to 30 ° C under aerobic conditions, and grows best at pH 7.0 and 28 ° C. Suitable culture media were TSB (Tryptic Soy Broth), TSA (Tryptic Soy Agar), NA (Nutrient Broth), and NA (Nutrient Agar), and both liquid shake culture and solid culture showed good growth of the strain. In addition, in order for the state of the strain to be optimally cultured, it was preferable to culture the pre-cultured strain in a new medium for 24 to 48 hours. At this time, the initial inoculation concentration of the strain in the medium was preferably 1x10 ^{4 to 5} CFU / mL.

Next, in order to confirm the genetic characteristics, the ObRS-5 strain was streaked on TSA medium and incubated at 28 ° C. for 24 hours, and then a single colony was inoculated in Tryptic Soybean Broth (TSB; Difco) medium at 28 ° C., 48 Incubated for hours. The strain culture was centrifuged at 8,000 rpm for 15 minutes to harvest the cells, and genomic DNA was extracted according to the manufacturer's manual using the Genomic DNA Prep kit (Nanohelix, Korea). To amplify the 16S rDNA base sequence of the extracted DNA, 27F (5'-GAGTTTGMTCCTGGCTCAG-3 '), 1492R (5'-ACGGYTACCTTGTTACGACTT-3') (M is A or C, Y is C or T) primer is used. (Weisburg et al., 1991) After heating at 95 ° C for 5 minutes, 30 reactions at 95 ° C for 1 minute, 55 ° C for 1 minute, 72 ° C for 1 minute and 30 seconds, and 72 ° C for 10 minutes To react. After confirming the length of the amplified PCR product by electrophoresis analysis, Genotech Co. (Daejeon, Korea) was commissioned to sequence analysis. To create a molecular sequence using the DNA sequence for 16S ribosomal RNA, the strain sequence was sorted using the MEGA 6.06 program, and then a phylogenetic tree was created using a neighbor-joining algorithm (Bootstrapping 1000 repetitions; Felsenstein, 1985) ). The base sequence of the compared standard strain was downloaded and used through the EzTaxon-e server (http://www.eztaxon.org).

The DNA nucleotide sequence (SEQ ID NO: 1) of the 16S rRNA gene of the ObRS-5 strain was identified as shown in FIG. 4, and as a result of creating a molecular system as shown in FIG. 5, belonging to a relationship with several strains of Enterobacter Appeared. Also ObRS-5 strain Enterobacter asburiae JCM 6052 strain and showed a high homology of 99.9% Bootstrap values are shown to be relatively high as 86%, are found to belong to the subcluster of Enterobacter asburiae, finally Enterobacter asburiae to ObRS-5 Pity.

<Example 5. Effect of control of seed coating of ObRS-5 strains to control the effect of controlling the disease of bear deodorant>

Soil treatment (inoculation) of Gomchi plague was conducted as follows. Since the host of the late blight Phytophthora drechselri is known to be the primary source of invasion of host plants, securing large amounts of zoospores in studies dealing with late blight is one of the important factors for conducting experiments (Mansoori and Banihashemi, 1982). First, in order to prepare the soil extract necessary for inducing the sacrum, the soil filtered through a 2 mm sieve was mixed with distilled water in a ratio of 1: 3 (v / v) and 3 g of CaCO ₃ was added to stand for 20 hours at room temperature, and 3 layers The filtrate from which the soil was filtered with gauze was collected, filtered through filter paper, and sterilized. After incubating the Phytophthora drechselri flora with a diameter of 5 mm in V8 juice medium (330 ml of V8 juice (Campbell's Soup Co., Camden, USA), CaCO ₃ 1.5 g, DW 670 ml) for 3 days, wash the medium with sterile water and wash the soil. After the extract was added, the mixture was allowed to stand still for 6 days while irradiating light at 20 ° C. for 24 hours / day. After confirming the presence or absence of a juvenile follicle, the microscope was checked with a stereomicroscope (M205A, LEICA, Germany), and treated at -20 ° C for 25 minutes to exit the jujuder, and then left at room temperature for 3 hours. After the mycelium was filtered with sterile gauze, the soil was irrigated with ^5.0 ml per week of soil to adjust the concentration of the owner's suspension to be 5.0x10 ⁵ zoospores per g of soil.

Next, the selected Enterobacter Asburier The anti-blight control effect of the ObRS-5 strain by coating the odorous seed was tested. Enterobacter asburier ObRS-5 strain was streaked on TSA medium, incubated at 28 ° C. for 24 hours, and then suspended in 0.1% peptone water to adjust the concentration to 1.0 × ^{10 8} cfu / ml. Sterile gauze was placed on sterilized topsoil (Baroker, Seoul Bio, Korea), and the surface sterilized bear seeds were sown, and then covered again with sterile soil. After 5 days of sowing, after 5 hours of sowing, the germ-bearing seeds with germination of 1.5-2 mm germinated were immersed in the Enterobacter asburier ObRS-5 strain suspension prepared for 3 hours, and then re-sown into a 32-hole tray (n = 12). Controls were sown after immersion in 0.1% peptone water. After 4 weeks of sowing, the incidence rate (%) was investigated when the phagocytosis Phytophthora drechselri was inoculated through the soil irrigation treatment presented above, and over 50% of the withered lesions appeared in the control.

The results of the incidence are shown in Table 1 and FIG. 6, and FIG. 6 shows Experiment 1 results of Table 1, wherein A is a seed-coated group of Enterobacter asburier ObRS-5 before treatment with the pathogen Phytophthora drechselri . , B represents the treatment group of Phytophthora drechselri , the pathogen, and C represents the untreated group. In addition, the control was also calculated using the results in Table 1.

Control = ((no treatment-treatment) / no treatment) x 100 (%)

As shown in Table 1 and FIG. 6, the incidence of late blight in the seed-coated group with Enterobacter asburier ObRS-5 strain is 25.0%, which is confirmed to have an effect of controlling deodorant plague of 60% (control). Even in the result of repeated executions, the group treated with Enterobacter asburier ObRS-5 strain exhibits a control effect of 60% of anemone plague.

Treatment Disease incidence (%) Experiment 1 Experiment 2 5 days after 15 days after 5 days after 15 days after ObRS-5 25.0 25.0 18.8 25.0 pathogen-treated control 56.3 62.5 62.5 62.5 nontreated healthy control 0 0 0 0

<Example 6. Control of the effect of controlling the disease of bear odor by irrigation treatment of ObRS-5 strain>

Enterobacter Asburier ObRS-5 In order to test the effect of controlling the deodorant plague by irrigation treatment, the ObRS-5 strain was inoculated in TSB medium and shaken and cultured for 180 rpm, 48 hours, and then the concentration was 1x10 ⁸ cfu / ml. Was adjusted to. The prepared Enterobacter Asburier ObRS-5 culture was irrigated once every 50 ml per week (Experiment 1 and 2 in Table 2) or twice at 7 day intervals (Experiment 3 in Table 2) at 12 weeks of sowing at 4 weeks after sowing. In the control group, TSB medium was irrigated in the same manner. Enterobacter Asburier ObRS-5 was inoculated with Phytophthora drechselri , as shown in Example 5, after 7 days of culture, and the incidence (%) was investigated when the withered withdrawal symptom was greater than 50% in the control. It is shown in FIG. 7.

7 is a result of Experiment 1 in Table 2, A and C are groups inoculated with the bacterium Phytophthora drechselri after the irrigation treatment of Enterobacter asburier ObRS-5 strain, and groups B and D were inoculated only with the bacterium Phytophthora drechselri to be.

Treatment Disease incidence (%) Experiment 1 Experiment 2 Experiment 3 7 days after 14 days after 7 days after 14 days after 7 days after 14 days after ObRS-5 0 7.1 0 6.3 11.1 11.1 Control 68.8 75.0 50.0 50.0 86.7 93.3

Referring to Table 2 and FIG. 7, as a result of assaying the effect of controlling the deodorant blight by soil irrigation treatment of Enterobacter Asburier ObRS-5 strain culture, Enterobacter Asburier ObRS-5 strain treatment group on the 14th day of treatment In the incidence rate is 6.3 ~ 7.1%, compared to the group treated with Enterobacter asburier ObRS-5 and not Phytophthora drechselri alone, it was confirmed that it has an antibacterial effect of 87.4 to 90.5% (control).

In addition, as a result of two soil irrigation treatments, the incidence rate of Enterobacter asburier ObRS-5 strain treatment was 11.1% on the 7th, which showed 87.2% (control) of the antibacterial effect of bear odor, 14 days after the inoculation It can be seen that 88.1% (control) showed the effect of controlling the plague plague.

As a result of the above, it was confirmed that Enterobacter asburier ObRS-5 strain control effect of controlling the antibacterial effect of bear odor was excellent.

National Academy of Agricultural Science KACC81075BP 20180703

<110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> New microorganism Enterobacter asburiae ObRS-5 or microbial agent          comprising the same <130> P-2018-0225 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1356 <212> DNA <213> Unknown <220> <223> Enterobacter asburiae <400> 1 ggttaagcta cctacttctt ttgcaaccca ctcccatggt gtgacgggcg gtgtgtacaa 60 ggcccgggaa cgtattcacc gtagcattct gatctacgat tactagcgat tccgacttca 120 tggagtcgag ttgcagactc caatccggac tacgacgcac tttatgaggt ccgcttgctc 180 tcgcgaggtc gcttctcttt gtatgcgcca ttgtagcacg tgtgtagccc tactcgtaag 240 ggccatgatg acttgacgtc atccccacct tcctccagtt tatcactggc agtctccttt 300 gagttcccgg ccggaccgct ggcaacaaag gataagggtt gcgctcgttg cgggacttaa 360 cccaacattt cacaacacga gctgacgaca gccatgcagc acctgtctca gagttcccga 420 aggcaccaat ccatctctgg aaagttctct ggatgtcaag agtaggtaag gttcttcgcg 480 ttgcatcgaa ttaaaccaca tgctccaccg cttgtgcggg cccccgtcaa ttcatttgag 540 ttttaacctt gcggccgtac tccccaggcg gtcgacttaa cgcgttagct ccggaagcca 600 cgcctcaagg gcacaacctc caagtcgaca tcgtttacgg cgtggactac cagggtatct 660 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tctttgtcca gggggccgcc 720 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc 780 cccctctaca agactctagc ctgccagttt cgaatgcagt tcccaggttg agcccgggga 840 tttcacatcc gacttgacag accgcctgcg tgcgctttac gcccagtaat tccgattaac 900 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg 960 ggtaacgtca atcgacaagg ttattaacct tatcgccttc ctccccgctg aaagtacttt 1020 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg 1080 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc 1140 tggtcatcct ctcagaccag ctagggatcg tcgcctaggt gagccgttac cccacctact 1200 agctaatccc atctgggcac atctgatggc aagaggcccg aaggtccccc tctttggtct 1260 tgcgacgtta tgcggtatta gctaccgttt ccagtagtta tccccctcca tcaggcagtt 1320 tcccagacat tactcacccg tccgccgctc gtcacc 1356

Claims (8)

Enterobacter asburiae ObRS-5, a new microorganism having a plant disease control efficacy, Enterobacter asburiae ObRS-5, microorganism accession number: KACC 81075BP. According to claim 1,
The plant disease is a strain characterized by being derived from Phytophthora drechsleri ( Pytopthora drechsleri ). According to claim 1,
The plant is a strain characterized in that it is selected from the group consisting of gomchi, pepper, tomato, cucumber, lettuce, watermelon, mustard, Angelica, onion, cabbage, green onion, radish, ginseng, strawberry, lettuce and garlic. According to claim 1,
The strain is characterized in that it comprises a gene of 16s rRNA represented by the nucleotide sequence of SEQ ID NO: 1. Claim 1 Enterobacter asburier ObRS-5 strain or a microbial agent for controlling plant diseases characterized in that it comprises a culture medium. A microbial pesticide comprising the microbial agent of claim 4. The method of claim 6,
The microbial pesticide is a microbial pesticide, characterized in that the formulation selected from the group consisting of seed coating agent, seed soaking agent, soil improver, composting agent, foliar sprayer and irrigation sprayer. Diluting the microbial agent of claim 4 by 10 to 1000 times to obtain a diluent; And, dipping the diluent into a plant to be controlled, seed immersion, seed coating, foliar spraying or irrigation spraying.

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