KR20200044202A - Water jacket apparatus of dispenser for keeping biomaterial constant temperature in bio 3 dimentional printer and multi stage temperature control method of the apparatus - Google Patents

Abstract

The present invention provides a water jacket apparatus of a dispenser for keeping constant temperature of a biomaterial in a three-dimensional bioprinter and a multi-stage temperature control method of the apparatus. The three-dimensional bioprinter includes a dispenser configured to receive a biomaterial contemporarily and to eject the biomaterial downwardly through a nozzle, and a water jacket coupled to the dispenser, wherein the dispenser includes: a sheet-like base plate; a pair of syringe fixing portions fixed to the base plate, formed to protrude out from the fixed surface in the vertical direction, and disposed in the form of a cantilever with a predetermined interval; and a syringe fixed elastically through press-fitting into the syringe fixing portions and provided with a biomaterial-receiving space therein. In addition, the water jacket is coupled detachably to the base plate, disposed to three-dimensionally surround the syringe, provided with a water-receiving space therein, and has a water inlet and a water outlet.

Description

Water jacket apparatus of dispenser for keeping biomaterial constant temperature in bio 3 dimentional printer and multi stage temperature control method of the apparatus}

The present invention relates to a bio three-dimensional printer capable of three-dimensional printing of biomaterials, and more specifically, disposed above an injection nozzle for jetting biomaterials to maintain a constant viscosity by maintaining the biomaterial at a constant temperature. It is about the device.

The three-dimensional printer includes a frame constituting the XYZ axis and a nozzle through which a liquid or powdery material capable of stacking three-dimensional shapes in a flat shape is injected. 3D printers include, for example, a Cartesian method, a Mendel method, a Delta method, and a Core XY method. The Cartesian method is a method in which the bed moves in the X and Y axes and the nozzle moves in the Z axis to realize 3D printing. The Mendel method is a method in which the bed moves in the Z axis and the nozzle moves in the X and Y axes to realize 3D printing. The delta method is a method in which the nozzle moves in the X, Y, and Z axes to implement 3D printing. In the core XY method, the nozzle is moved in the X and Y axis directions by a belt connected by two motors, and the bed is moved in the Z direction. The core XY method, which can most accurately control the position of the nozzle, has been widely used.

The bio-three-dimensional printer is a device structurally modified to eject biomaterial through a nozzle of a three-dimensional printer to create three-dimensional biological tissues or organs.

The bio-three-dimensional printer includes a nozzle in the form of a dispenser for discharging biomaterials. The bio 3D printer fills the nozzle with a biomaterial in the form of a viscous fluid such as collagen or gelatin. It connects a pneumatic system to a nozzle filled with a fluid-type biomaterial and pushes the biomaterial while discharging it.

An example of such a bio 3D printer is disclosed in Korean Patent Registration No. 1828345.

Since the bio-material contained in the receiving space that accommodates the bio-material before the bio-material is discharged from the bio-three-dimensional printer, most of the bio-material contains protein, from the sol state, which is fluid by heat, to the gel state, which is not fluid. Changes. Therefore, the biomaterial needs to be maintained at a constant temperature before being injected through the nozzle. Most biomaterials should be maintained at a constant temperature within a range of 4 ° C to 50 ° C depending on the type.

The biomaterial accommodated in the dispenser portion is discharged to the bioware fixed on the bed through the nozzle, and then changed to a gel state by ultraviolet light irradiated from the ultraviolet lamp disposed under the dispenser portion to obtain a three-dimensional structure. To form.

In order to achieve this purpose, a conventional bio 3D printer employs a Peltier device to maintain a constant temperature of a dispenser containing a biomaterial before the biomaterial is ejected through a nozzle. However, when employing a Peltier element, there is a problem that it is difficult to downsize the device. In addition, since the Peltier element needs to be controlled electronically, there is a problem in that the control circuit must be complicated. In addition, when configured to control the temperature of the dispenser using a Peltier element, there is a problem that the volume of the device becomes large and heavy.

The object of the present invention is to solve the problems as described above, by improving the structure of maintaining a constant temperature of the dispenser portion of the bio three-dimensional printer, constant temperature using water without using a bulky Peltier element It is to provide a dispenser unit water jacketing device for biomaterial constant temperature of a bio three-dimensional printer that maintains and a method of controlling the temperature of the water jacket.

In order to achieve the above object, the dispenser unit water jacketing device for constant temperature of biomaterials in a bio three-dimensional printer according to an embodiment of the present invention is a device constituting a bio three-dimensional printer, and temporarily accommodates biomaterials, through a nozzle. And a dispenser unit configured to discharge the biomaterial downward and a water jacket coupled to the dispenser unit,

The dispenser portion includes a plate-shaped base plate;

A syringe fixing part fixed to the base plate and protruding in a direction perpendicular to the fixing surface, disposed in a cantilevered form to form a pair of pillars having a constant interval;

Includes a syringe that is elastically fixed in a form forcibly fitted to the syringe fixing part and is provided with a space for receiving biomaterial therein.

The water jacket is detachably coupled to the base plate, is arranged to surround the syringe in three dimensions, is provided with a water receiving space for receiving water therein, and an inlet for water inlet and an outlet for water outlet. There are features.

A plurality of engaging projections disposed along both sides of the base plate and protruding in a direction parallel to the syringe fixing part; And

It is provided on the water jacket; a plurality of coupling grooves formed concavely to slidably receive the coupling protrusion;

The water jacket is assembled so that the engaging groove portion is coupled to the engaging projection,

It is preferable that the water jacket is slidably coupled to the syringe fixing part while being in pressure contact with a side surface of the syringe fixing part.

The base plate is formed to extend in the longitudinal direction of the syringe from the syringe fixing portion and includes a syringe support portion for three-dimensionally supporting the back surface of the syringe,

It is preferable that the side of the syringe support and the water jacket are combined to have an attractive force by magnetic force.

A chiller installed on the outside of the 3D bio printer, which accommodates the water flowing into the water jacket and is connected to the inlet and the outlet with flexible hoses, and each has a hot water storage tank and a cold water storage tank maintained at a constant temperature. Device; And

It is preferable to include a mixing valve device for proportionally mixing hot and cold water supplied from the chiller device.

The mixing valve device includes a direct current motor;

A reduction gear coupled with the rotating shaft of the DC motor to increase torque and reduce rotation speed;

A sealing part disposed between the DC motor and the reduction gear to prevent the DC motor from being contaminated by moisture;

A fixed body installed to be fixed to the DC motor; And

Includes; movable body rotatably installed on the fixed body,

The fixed body is arranged to be spaced apart from the hot water flow path through which the hot water flows, and the hot water flow path along the rotational direction of the movable body, and the cold water flow path through which cold water flows and the mixed flow path disposed opposite the hot water flow path and the cold water flow path. Equipped,

The hot water flow path and the extension line of the cold water flow path are formed to intersect at one point,

The movable body includes a first movable passage corresponding to the hot water passage, a second movable passage corresponding to the cold water passage, and a third movable passage corresponding to the mixed passage,

The first movable flow passage and the second movable flow passage join to form a third movable flow passage,

When the center line of the first movable flow path and the center line of the hot water flow path coincide with each other, the connection between the second movable flow path and the cold water flow path is completely blocked,

When the center line of the second movable channel and the center line of the cold water channel coincide with each other, the connection between the first movable channel and the hot water channel is completely blocked,

When the first movable passage and the hot water passage are partially communicated, the second movable passage and the cold water passage are also partially communicated, and the sum of the hot water and cold water flowing into the first movable passage and the second movable passage is always Constant,

The third movable flow path always communicates with the mixed flow path in a position where the first movable flow path or the second movable flow path communicates with the hot water flow path or the cold water flow path,

It is preferable that the third movable flow path is configured to be blocked from the mixed flow path in a position where the first movable flow path and the second movable flow path are completely blocked from the hot water flow path and the cold water flow path.

In order to achieve the above object, the multi-temperature control method of the dispenser unit water jacketing device for constant temperature of a biomaterial of a bio three-dimensional printer according to an embodiment of the present invention temporarily accepts biomaterial and downwards through a nozzle. A flexible hose through a mixing valve device with a chiller device for receiving water flowing into the water jacket and an inlet of the water jacket to supply water at a constant temperature to a water jacket coupled to a dispenser unit configured to discharge substances Influent connection step to connect to;

A discharge water connection step of connecting the outlet of the water jacket and the chiller device with a hose; And

It includes; a temperature control step of controlling the temperature of the dispenser portion to a constant temperature by proportionally mixing the hot and cold water supplied from the chiller device in the mixing valve device to the inlet;

The mixing valve device,

A fixed body installed to be fixed to the DC motor; And

Includes; movable body rotatably installed on the fixed body,

The fixed body is arranged to be spaced apart from the hot water flow path through which the hot water flows, and the hot water flow path along the rotational direction of the movable body, and the cold water flow path through which cold water flows and the mixed flow path disposed opposite the hot water flow path and the cold water flow path. Equipped,

The hot water flow path and the extension line of the cold water flow path are formed to intersect at one point,

The movable body includes a first movable passage corresponding to the hot water passage, a second movable passage corresponding to the cold water passage, and a third movable passage corresponding to the mixed passage,

The first movable flow passage and the second movable flow passage join to form a third movable flow passage,

When the center line of the first movable flow path and the center line of the hot water flow path coincide with each other, the connection between the second movable flow path and the cold water flow path is completely blocked,

When the center line of the second movable channel and the center line of the cold water channel coincide with each other, the connection between the first movable channel and the hot water channel is completely blocked,

When the first movable passage and the hot water passage are partially communicated, the second movable passage and the cold water passage are also partially communicated, and the sum of the hot water and cold water flowing into the first movable passage and the second movable passage is always Constant,

The third movable flow path always communicates with the mixed flow path in a position where the first movable flow path or the second movable flow path communicates with the hot water flow path or the cold water flow path,

The third movable flow path is configured to be blocked from the mixed flow path in a position where the first movable flow path and the second movable flow path are completely blocked from the hot water flow path and the cold water flow path.

In order to achieve the above object, the multi-temperature control method of the dispenser unit water jacketing device for constant temperature of biomaterials in a bio three-dimensional printer according to another embodiment of the present invention temporarily accommodates biomaterials and downwards through a nozzle. Inlet water connection step of connecting the inlet of the water jacket and the chiller device for receiving the water flowing into the water jacket with a flexible hose to supply water of a constant temperature to the water jacket coupled to the dispenser unit configured to discharge the material. ;

A discharge water connection step of connecting the outlet of the water jacket and the chiller device with a hose; And

Includes; a temperature control step of controlling the temperature of the dispenser portion to a constant temperature by mixing the hot and cold water supplied from the chiller device in a Y-shaped mixing connector tube and supplying it to the inlet.

The chiller device includes a hot water water pump for forcibly feeding hot water to the mixing connector tube, and a cold water water pump for forcibly forcing cold water to the mixing connector tube,

 When the temperature of the water jacket is lower than a preset temperature, the hot water pump is operated,

When the temperature of the water jacket is higher than a preset temperature, the cold water pump is operated,

When the temperature of the water jacket is the same as the preset temperature, the hot water water pump and the cold water water pump are configured to operate simultaneously.

The dispenser part water jacketing device for constant temperature of biomaterial of the bio three-dimensional printer according to the present invention is provided with a water jacketing device detachably coupled to the dispenser part, so that the water jacketing device can be easily separated from the base plate when the syringe is exchanged. Syringe exchange provides a very convenient effect. In addition, the dispenser unit water jacketing device according to the present invention has the advantage of easily miniaturizing a three-dimensional bio-printer device because it is possible to precisely control the temperature of the dispenser unit by mixing hot and cold water without using a conventional Peltier element. In addition, there is an advantage that does not require a complex electronic circuit for controlling the Peltier element. In addition, the multi-temperature control method of the dispenser unit water jacketing device for constant temperature of biomaterials in a bio three-dimensional printer stores hot and cold water supplied to the water jacket in a chiller device as an external independent device, and is very simple by a DC motor. By adopting a method of supplying water maintained at a constant temperature to the water jacket through a mixing valve device capable of precisely mixing hot and cold water proportionally with a structure or a Y-shaped mixing connector tube, the temperature of the dispenser portion is multi-staged. It provides an effect that can be very precisely controlled.

1 is a view showing the configuration of a dispenser unit water jacketing device for constant temperature of biomaterials in a bio three-dimensional printer according to a preferred embodiment of the present invention.
Figure 2 is an exploded perspective view of the main components of the dispenser portion and the water jacket shown in Figure 1;
3 is an exploded perspective view of the mixing valve device shown in FIG. 1.
4 is a cross-sectional view taken along line IV-IV shown in FIG. 1.
5 is a cross-sectional view taken along line V-V shown in FIG. 1.
6 is a cross-sectional view taken along line VI-VI shown in FIG. 1.
7 is a view showing a state in which only hot water is supplied in FIG. 6.
8 is a view showing a state in which hot water and cold water are proportionally mixed in FIG. 6.
9 is a view showing a state in which supply of hot and cold water is blocked in FIG. 6.
10 is a flow chart showing a process diagram of a multi-control temperature method according to the present invention.
11 is a block diagram of an apparatus for explaining a multi-temperature control method of a water jacket of a dispenser unit according to another embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a view showing the configuration of a dispenser unit water jacketing device for constant temperature of biomaterials in a bio three-dimensional printer according to a preferred embodiment of the present invention. Figure 2 is an exploded perspective view of the main components of the dispenser portion and the water jacket shown in Figure 1; 3 is an exploded perspective view of the mixing valve device shown in FIG. 1. 4 is a cross-sectional view taken along line IV-IV shown in FIG. 1. 5 is a cross-sectional view taken along line V-V shown in FIG. 1. 6 is a cross-sectional view taken along line VI-VI shown in FIG. 1. 7 is a view showing a state in which only hot water is supplied in FIG. 6. 8 is a view showing a state in which hot water and cold water are proportionally mixed in FIG. 6. 9 is a view showing a state in which supply of hot and cold water is blocked in FIG. 6. 10 is a flow chart showing a process diagram of a multi-control temperature method according to the present invention. 11 is a block diagram of an apparatus for explaining a multi-temperature control method of a water jacket of a dispenser unit according to another embodiment of the present invention.

1 to 9, a dispenser unit water jacketing device (hereinafter referred to as “the dispenser water jacketing device”) for biomaterial constant temperature of a bio three-dimensional printer according to a preferred embodiment of the present invention is a bio three-dimensional printer. (Hereinafter referred to as a "bio 3D printer").

The dispenser part water jacketing device 10 includes a dispenser part 20, a water jacket 50, a mixing valve device 60, and a chiller device 70.

The dispenser unit 20 temporarily receives biomaterial. The dispenser unit 20 is configured to discharge the biomaterial downward through the injection nozzle 40. The discharged biomaterial constitutes a three-dimensional shape in a state accommodated in the bio-wear 110 installed in the bed 100 disposed under the dispenser unit 20.

The dispenser portion 20 includes a base plate 22, a syringe fixing portion 24, a syringe 30, an engaging projection 28, and a spray nozzle 40.

The base plate 22 is a plate-like structure. The base plate 22 may be made of stainless steel, carbon steel, or aluminum alloy. For example, the base plate 22 may be a rectangular structure. The base plate 22 constitutes a head portion of the bio 3D printer. Therefore, the base plate 22 is installed to move in the X and Y axis directions. A known core XY method may be applied to the method in which the base plate 22 moves.

The syringe fixing part 24 is fixed to the base plate 22. The syringe fixing portion 24 is provided to detachably fix the syringe 30 to be described later. The syringe fixing part 24 is formed to protrude in a direction perpendicular to the fixing surface of the base plate 22. The syringe fixing part 24 is arranged in a cantilevered form and constitutes a pair of pillars having regular intervals. The syringe fixing part 24 may be fixed to the base plate 22 by bolts or screws. The inner surface of the syringe fixing portion 24 has a circular cross-sectional structure to correspond to the shape of the syringe 30. On the other hand, the outer surface of the syringe fixing portion 24 has a cross-sectional structure in a flat shape so that the water jacket 50, which will be described later, can be pressed and slidingly engaged. The syringe fixing part 24 is coupled to the syringe 30 is elastically forcibly fitted.

The syringe 30 is a syringe-shaped structure. The syringe 30 may be configured to press the biomaterial by a piston, or may be configured to apply a constant pressure to the biomaterial by pneumatic pressure. The syringe 30 is elastically fixed in a form forcibly fitted to the syringe fixing part 24. A space for accommodating biomaterial is provided in the syringe 30. The lower portion of the syringe 30 is provided with a discharge needle protruding downward. The discharge needle may be connected to the injection nozzle 40 and an element such as a Teflon tube.

The base plate 22 may include a syringe support 26. The syringe support 26 is formed to extend from the syringe fixing portion 24 in the longitudinal direction of the syringe 30. The syringe support part 26 supports the back surface of the syringe in three dimensions. The syringe support portion 26 has an arc-shaped cross section to support the syringe 30 in three dimensions.

A plurality of the engaging projections 28 are provided. The engaging projection 28 is disposed spaced apart along the boundary on both sides of the base plate (22). One end of the engaging projection 28 is fixed to the base plate 22. The other end of the engaging projection 28 is formed to protrude in a direction parallel to the syringe fixing portion 24.

The injection nozzle 40 is disposed under the dispenser unit 20. The injection nozzle 40 is a discharge device that controls the minute amount of bio-material to be injected under the dispenser unit 20 by pulse control. The jet nozzle 40 may employ a jet nozzle having a known structure.

The water jacket 50 is detachably coupled to the dispenser portion 20. The water jacket 50 is provided with a water receiving space in which water can be accommodated. The water jacket 50 may be manufactured by a 3D printer. The water jacket 50 is arranged to surround the syringe 30 in three dimensions. More specifically, the water receiving space of the water jacket 50 may be arranged to surround both sides and the front surface of the syringe 30. The water jacket 50 is provided with an inlet 54 for water inflow and an outlet 56 for water discharge for discharging water accommodated in the water jacket 50 to the outside so that water can be introduced from the outside. The inlet 54 and the outlet 56 may be arranged side by side on top of the water jacket 50. The water jacket 50 is detachably coupled to the base plate 22. The water jacket 50 includes a plurality of coupling grooves 52. The coupling groove 52 is formed in the water jacket 50. More specifically, the engaging groove portion 52 is formed concave on the surface facing the water jacket 50 and the base plate 22. The engaging groove portion 52 is formed concave to accommodate the engaging projection 28 slidably. The water jacket 50 is assembled such that the engaging groove portion 52 is coupled to the engaging projection 28. The water jacket 50 is slidingly coupled to the syringe fixing portion 24 while being pressed against the side surface of the syringe fixing portion 24. The water jacket 50 is detachably coupled to the base plate 22 by such a coupling structure.

The side surface of the syringe support 26 and the water jacket 50 may be combined to have an attractive force by magnetic force. For example, a ferromagnetic plate is attached to the side surface of the syringe support part 26, and by attaching a magnetic plate to the ferromagnetic plate to face the ferromagnetic plate on the inner surface of the water jacket 50, the ferromagnetic plate and the magnet plate are attracted to each other, so that the water jacket ( 50) can be fixed to the base plate 22 more firmly.

The mixing valve device 60 is a device for proportionally mixing and discharging hot and cold water to maintain a constant temperature of water supplied to the water jacket 50. The mixing valve device 60 is connected to an inlet 54 provided in the water jacket 50 and a hose 80. The hose 80 is preferably made of a flexible material. The chiller device 70 for supplying hot and cold water to the mixing valve device 60 will be described later.

The mixing valve device 60 includes a DC motor 62, a reduction gear 64, a sealing portion 63, a fixed body 65 and a movable body 67.

The DC motor 62 is a device that generates rotational motion by DC power. The DC motor 62 may be a known motor.

The reduction gear 64 is mechanically coupled to the rotating shaft of the DC motor 62. The reduction gear 64 increases the torque of the rotating shaft of the DC motor 62 and decreases the number of revolutions. The reduction gear 64 may adopt a known structure.

The sealing portion 63 is disposed between the DC motor 62 and the reduction gear 64. The sealing portion 63 is a kind of sealing member that performs a dustproof and waterproof action to prevent the DC motor 62 from being contaminated by moisture. The sealing portion 63 may be made of rubber or silicone resin.

The fixed body 65 is installed to be fixed to the DC motor 62. The fixed body 65 is provided with a plurality of through holes 656. It is fixed to a fixing column 66 protruding toward the fixing body 65 from the DC motor 62 or the sealing portion 63 at a position corresponding to the through hole 656. An inner circumferential surface of the fixing column 66 forms a hollow portion, and a female screw portion is formed. The fixed body 65 is seated at the end of the fixed column 66, penetrates the through-hole 656 and is screwed to the fixed column 66 by the fixing bolt 700, the DC motor 62 ).

The fixed body 65 includes a movable body receiving portion 650, a hot water flow path 651, and a cold water flow path 653.

The movable body accommodating portion 650 may be configured as a cylinder-shaped groove portion in which the upper portion is closed at the central portion of the fixed body 65 and opened downward. The movable body accommodating portion 650, the movable body 67 to be described later is rotatably installed.

The hot water flow path 651 is a flow path penetrated to be connected to the movable body receiving portion 650 from the outer surface of the movable body 67. Hot water flows into the hot water flow path 651.

The cold water flow path 653 is a flow path penetrated to be connected to the movable body receiving portion 650 from the outer surface of the movable body 67. Cold water flows into the cold water flow path 653. The cold water flow path 653 is disposed to be spaced apart from the hot water flow path 651 along the rotational direction of the movable body 67. One mixing channel 655 is disposed opposite the hot water channel and the cold water channel 653. The mixing flow passage 655 is a flow passage formed to be connected to the movable body accommodating part 650 from the outer surface of the fixed body 65.

Extension lines of the hot water flow path 651 and the cold water flow path 653 are formed to intersect at one point. More specifically, a point at which the extension line of the hot water flow path 651 and the cold water flow path 653 intersect is present in the movable body accommodating part 650.

The movable body 67 is rotatably installed on the fixed body 65.

The movable body 67 is a columnar structure. A ring-shaped sealing ring 68 is installed on the outer circumferential surface of the movable body 67 so that hot water, cold water, or mixed water does not leak to a portion facing the fixed body 65. An outer circumferential surface of the movable body 67 is concavely formed at a portion where the sealing ring 68 is installed, and a sealing ring receiving groove extending around the outer circumferential surface is provided. The sealing ring 68 rotates integrally with the movable body 67. The center of the movable body 67 is fixed to the rotation axis of the reduction gear 64 to rotate integrally with the rotation axis of the reduction gear 64.

The movable body 67 includes a first movable flow passage 671, a second movable flow passage 673, and a third movable flow passage 675.

The first movable passage 671 corresponds to the warm water passage 651. The inlet of the first movable flow path 671 is opened through the outer peripheral surface of the movable body 67. The second movable flow path 673 corresponds to the cold water flow path 653. The second movable flow path 673 is opened through the outer circumferential surface of the movable body 67. The third movable flow passage 675 is a flow passage formed by the first movable flow passage 671 and the second movable flow passage 673 joining. The third movable flow path 675 is disposed to correspond to the mixed flow path 655. The first movable flow path 671, the second movable flow path 673, and the third movable flow path 675 cooperate to form a Y-shaped structure. The outlet of the third movable flow passage 675 is opened through the outer peripheral surface of the movable body 67. The cross-sectional area of the third movable flow passage 675 is formed larger than the cross-sectional area of the mixed flow passage 655.

When the center line of the first movable channel 671 and the center line of the warm water channel 651 coincide with each other, the connection between the second movable channel 673 and the cold water channel 653 is completely blocked. On the other hand, when the center line of the second movable flow path 673 and the center line of the cold water flow path 653 coincide with each other, the connection between the first movable flow path 671 and the hot water flow path 651 is completely blocked. Meanwhile, when the first movable passage 671 and the hot water passage 651 are partially communicated, the second movable passage 673 and the cold water passage 653 are also arranged to be partially communicated. When the hot water flow passage 651 and the first movable flow passage 671 communicate, or when the cold water flow passage 653 and the second movable flow passage 673 are communicated, the first movable flow passage 671 and the second The sum of hot water and cold water flowing into the movable flow path 673 is arranged to be kept constant at all times.

The third movable flow passage 675 is always the mixed flow passage (675) at a position where the first movable flow passage 671 or the second movable flow passage 673 communicates with the hot water flow passage 651 or the cold water flow passage 653. 655).

On the other hand, the third movable flow path 675 is located at a position where the first movable flow path 671 and the second movable flow path 673 are completely blocked from the hot water flow path 651 and the cold water flow path 653. It is configured to be blocked with the mixing channel 655. Accordingly, as the movable body 67 is rotated by the DC motor 62, hot water and cold water introduced through the hot water flow path 651 and the cold water flow path 653 are proportionally mixed and the mixing flow path ( 655). For example, when the hot water supplied to the hot water flow path 651 is 50 ° C. and the cold water flowing through the cold water flow path 653 is 4 ° C., the temperature of the mixed water discharged through the mixing valve device 60 is discharged. Can be precisely controlled in the range from 4 ° C to 50 ° C. The mixing valve device 60 is structurally simple and implements an effect of controlling the temperature of the mixed water very precisely in multiple stages.

The chiller device 70 is a device that maintains water supplied to the water jacket 50 at a constant temperature. The chiller device 70 includes a hot water storage tank connected to a hot water flow path 651 of the mixing valve device 60 by a hose 80. In addition, the chiller device 70 is provided with a cold water storage tank connected to the cold water flow path 653 of the mixing valve device 60. The hot and cold water supplied from the chiller device 70 is proportionally mixed in the mixing valve device 60. The hot water storage tank (not shown) and the cold water storage tank (not shown) each independently maintain a constant temperature of water stored by a heat exchanger configured independently of each other. For example, the hot water storage tank may be maintained at 50 ° C, and the cold water storage tank may be maintained at 4 ° C.

The hot water storage tank and the cold water storage tank of the chiller device 70 are connected to the inlet 54 and the outlet 56 of the water jacket 50 by a flexible hose 80 via the mixing valve device 60. Can be connected. The chiller device 70 may be independently installed outside the 3D bio printer.

The chiller device 70 is a hot water water pump (not shown) that forcibly presses the water in the hot water storage tank toward the water jacket 50 and cold water that forces the water in the cold water storage tank toward the water jacket 50. A water pump (not shown) may be provided. The hot water pump and the cold water pump can be precisely controlled by, for example, a PID control method.

The mixing valve device 60 may be replaced with a Y-shaped mixing connector tube 90 as shown in FIG. 11 as necessary. The mixing connector tube 90 includes an inlet portion into which hot water and cold water are respectively separated and an outlet portion through which the hot and cold water is mixed and discharged. The inlet parts of the mixing connector tube 90 may be connected to the hot water storage tank and the cold water storage tank, respectively, with flexible hoses 80. In addition, the outlet portion of the mixing connector tube 90 may be connected to the inlet 54 and the hose 80 of the water jacket 50.

Hereinafter, a multi-temperature control method of the water jacketing device 10 of the dispenser unit will be described in detail using a device including the above-described components.

As illustrated in FIG. 10, the multi-temperature control method includes an influent connection step (S100), an exhaust water connection step (S200), and a temperature control step (S300).

 In the step of connecting the influent water (S100), the inlet 54 of the water jacket and the chiller device 70 for receiving the water flowing into the water jacket are connected by a flexible hose through the mixing valve device 60. . More specifically, the hot water storage tank of the chiller device 70 and the hot water channel 651 of the mixing valve device 60 are connected by a hose 80. In addition, the cold water storage tank of the chiller device 70 and the cold water flow path 653 of the mixing valve device 60 are connected with a hose. The mixing passage 655 of the mixing valve device 60 and the inlet 54 of the water jacket 50 are connected by a hose 80.

That is, in the step of connecting the influent (S100), the biomaterial is temporarily received, and water of a constant temperature is supplied to the water jacket 50 coupled to the dispenser unit 20 configured to discharge the biomaterial downward through a nozzle. So that the inlet 54 of the water jacket 50 and the chiller device 70 for receiving the water flowing into the water jacket 50 through the mixing valve device 60 to the flexible hose 80 Connect.

In the discharge water connection step (S200), the outlet 56 of the water jacket 50 and the chiller device 70 are connected with a hose. More specifically, the outlet portion 56 of the water jacket 50 and the return portion of the chiller device 70 are connected by a hose 80. The water recovered through the return unit 72 may be branched and stored in the hot water storage tank or the cold water storage tank.

In the temperature control step (S300), the hot water and cold water supplied from the chiller device 70 are proportionally mixed in the mixing valve device 60 and supplied to the inlet 54. In the temperature control step (S300), for example, the hot water water pump and the cold water water pump are simultaneously operated so that pressures of hot water and cold water supplied to the mixing valve device 60 are constantly generated.

In the temperature control step (S300), the temperature of the dispenser unit 20 is controlled to a constant temperature. More specifically, the temperature of the hot water accommodated in the chiller device 70 is maintained at 50 ° C. In addition, the temperature of the hot water accommodated in the chiller device 70 is configured to maintain 4 ° C. Hereinafter, how the hot water and the cold water are proportionally mixed in the mixing valve device 60 will be described in detail. Hot water is supplied to the hot water flow path 651 from the hot water storage of the chiller device 70. In addition, cold water is supplied to the cold water flow path 653 from the cold water storage of the chiller device 70. The movable body 67 is rotatably installed with respect to the fixed body 65. When the temperature of the water jacket 50 is to be maintained at 50 ° C., the movable body 67 moves the DC motor 62 so that the center line of the hot water flow path 651 coincides with the center line of the first movable flow path 671. ) To rotate. As described above, when the center line of the first movable channel 671 and the center line of the hot water channel 651 coincide with each other, the connection between the second movable channel 673 and the cold water channel 653 is completely blocked. Therefore, the temperature of the water supplied to the inlet 54 of the water jacket 50 through the third movable flow passage 675 and the mixing flow passage 655 is kept constant at 50 ° C. Accordingly, the temperature of the water jacket 50 can be maintained at 50 ° C. Accordingly, the biomaterial accommodated in the syringe 30 of the dispenser unit 20 in heat exchange with the water jacket 50 may be maintained at 50 ° C.

Meanwhile, when the temperature of the water jacket 50 is to be maintained at 4 ° C., the movable body 67 is connected to the DC motor so that the center line of the cold water flow path 653 coincides with the center line of the second movable flow path 673. Operate (62) to rotate. As described above, when the center line of the second movable channel 673 and the center line of the cold water channel 653 coincide with each other, the connection between the first movable channel 671 and the hot water channel 651 is completely blocked. Therefore, the temperature of the water supplied to the inlet 54 of the water jacket 50 through the third movable flow passage 675 and the mixing flow passage 655 is kept constant at 4 ° C. Accordingly, the temperature of the water jacket 50 can be maintained at 4 ° C. Accordingly, the biomaterial accommodated in the syringe 30 of the dispenser portion 20 in heat exchange with the water jacket 50 may be maintained at 4 ° C.

Meanwhile, when the temperature of the water jacket 50 is to be maintained at 27 ° C., the movable body 67 is connected so that the cross-sectional area of the hot water flow path 651 and the first movable flow path 671 communicate only 50%. The DC motor 62 rotates. In this case, the cold water flow path 653 communicates with the second movable flow path 673 through the remaining 50% cross-sectional area. Accordingly, water supplied to the water jacket 50 through the third movable flow passage 675 and the mixing flow passage 655 is supplied in a state where hot water and cold water are mixed at a ratio of 50:50. Accordingly, the temperature of the water jacket 50 can be maintained at 27 ° C. Accordingly, the biomaterial accommodated in the syringe 30 of the dispenser unit 20 in heat exchange with the water jacket 50 may be maintained at 27 ° C.

As described above, as the movable body 67 is rotated to a certain specific position, the amount of hot water and cold water supplied to the first movable flow passage 671 and the second movable flow passage 673 can be precisely and proportionally adjusted. Therefore, the temperature of the water supplied to the water jacket 50 through the mixing channel 655 can be very precisely controlled in multiple stages.

In addition, when it is not necessary to control the temperature of the dispenser portion 20, the movable body 67 of the mixing valve device 60 is rotated to turn the hot water flow path 651 and the first movable flow path 671 and The cold water flow path 653 and the second movable flow path 673 move to a position that is not in full communication. In this case, since the communication between the mixing channel 655 and the third movable channel 675 is completely blocked, there is an effect of blocking water supplied to the water jacket 50 even without a separate shut-off valve.

Meanwhile, the amount of water supplied from the chiller device 70 to the water jacket 50 through the mixing valve device 60 is recovered through the return portion of the chiller device 70 through the outlet 56.

Meanwhile, a multi-temperature control method of the water jacket device 10 of the dispenser according to another embodiment of the present invention will be described.

As illustrated in FIG. 10, the multi-temperature control method of the water jacketing device 10 of the dispenser unit according to another embodiment includes the influent connection step (S100), the discharge water connection step (S200), and temperature The points including the control step (S300) are the same.

However, as shown in FIG. 11, the mixing connector tube 90 having a Y-shape is employed instead of the mixing valve device 60 in the inflow water connection step (S100).

In the step of connecting the influent water (S100), the inlet 54 of the water jacket and the chiller device 70 for receiving water flowing into the water jacket are flexible through the Y-shaped mixing connector tube 90. Connect with a hose. More specifically, the hot water storage tank of the chiller device 70 and one of the inlets of the mixing connector tube 90 are connected by a hose 80. In addition, the cold water storage tank of the chiller device 70 and the other inlet of the mixing connector tube 90 are connected by a hose 80. The outlet of the mixing connector tube 90 and the inlet 54 of the water jacket 50 are connected by a hose 80.

That is, in the step of connecting the influent (S100), the biomaterial is temporarily received, and water of a constant temperature is supplied to the water jacket 50 coupled to the dispenser unit 20 configured to discharge the biomaterial downward through a nozzle. So that the inlet 54 of the water jacket 50, and the chiller device 70 for receiving the water flowing into the water jacket 50, the flexible hose 80 through the mixing connector tube (90) Connect with.

In the discharge water connection step (S200), the outlet 56 of the water jacket 50 and the chiller device 70 are connected with a hose. More specifically, the outlet portion 56 of the water jacket 50 and the return portion of the chiller device 70 are connected by a hose 80. The water recovered through the return unit 72 may be branched and stored in the hot water storage tank or the cold water storage tank.

In the temperature control step (S300), hot water and cold water supplied from the chiller device 70 are mixed in the mixing connector tube 90 and supplied to the inlet 54. In the temperature control step (S300), the temperature of the dispenser unit 20 is controlled to a constant temperature. More specifically, the temperature of the hot water accommodated in the chiller device 70 is maintained at 50 ° C. In addition, the temperature of the hot water accommodated in the chiller device 70 is configured to maintain 4 ° C. Hereinafter, how the temperature of the water jacket 50 is controlled will be described in detail. When the temperature of the water jacket 50 is lower than a preset temperature, the hot water pump is operated. Accordingly, hot water is supplied to the water jacket 50 through the mixing connector tube 90 in the hot water storage of the chiller device 70. Accordingly, the temperature of the water jacket 50 gradually approaches the preset temperature. Meanwhile, when the temperature of the water jacket 50 is higher than a preset temperature, the cold water water pump is operated. Accordingly, cold water is supplied to the water jacket 50 through the mixing connector tube 90 in the cold water reservoir of the chiller device 70. Accordingly, the temperature of the water jacket 50 gradually approaches the preset temperature. In addition, when the temperature of the water jacket 50 is the same as a preset temperature, the hot water water pump and the cold water water pump are simultaneously operated. Accordingly, hot water and cold water are equally supplied to the mixing connector tube 90 in the hot water storage tank and the cold water storage of the chiller device 70 and then mixed and supplied to the water jacket 50. Accordingly, the temperature of the water jacket 50 may be maintained at a preset temperature. If the temperature of the water jacket 50 is lower or higher than a preset temperature, the hot water water pump or the cold water water pump is selectively operated as described above. As described above, the hot water water pump and the cold water water pump can precisely control the operation using, for example, a PID control method.

As described above, the temperature of the water jacket 50 is precisely controlled to a preset temperature by precisely controlling the amount of hot and cold water supplied to the mixing connector tube 90 by the hot water water pump and the cold water water pump. can do.

In addition, when there is no need to control the temperature of the dispenser unit 20, the operation of the hot water water pump and the cold water water pump may be stopped.

Meanwhile, the amount of water supplied from the chiller device 70 to the water jacket 50 through the mixing connector tube 90 is recovered through the return portion of the chiller device 70 through the outlet 56.

As described above, the dispenser part water jacket device according to the present invention is provided with a water jacket device detachably coupled to the dispenser part, so that the water jacket device can be easily separated from the base plate when the syringe is exchanged, so the syringe exchange operation is very easy. Provides a simple effect. In addition, the dispenser unit water jacketing device according to the present invention has the advantage of easily miniaturizing a three-dimensional bio-printer device because it is possible to precisely control the temperature of the dispenser unit by mixing hot and cold water without using a conventional Peltier element. In addition, there is an advantage that does not require a complex electronic circuit for controlling the Peltier element. In addition, the multi-temperature control method of the dispenser unit water jacketing device for constant temperature of biomaterials in a bio three-dimensional printer stores hot and cold water supplied to the water jacket in a chiller device as an external independent device, and is very simple by a DC motor. By adopting a method of supplying water maintained at a constant temperature to the water jacket through a mixing valve device capable of precisely mixing hot and cold water proportionally with a structure or a Y-shaped mixing connector tube, the temperature of the dispenser portion is multi-staged. It provides an effect that can be very precisely controlled.

As described above, the present invention has been described with reference to preferred embodiments, but the present invention is not limited by such examples, and various types of embodiments may be embodied within the scope without departing from the technical spirit of the present invention.

10: water jacket device
20: dispenser unit
22: base plate
24: syringe fixing part
26: syringe support
28: engaging projection
30: syringe
40: spray nozzle
50: water jacket
52: engaging groove
54: inlet
56: outlet
60: mixing valve device
62: DC motor
63: seal
64: reduction gear
65: fixed body
66: fixed column
67: movable body
68: sealing ring
70: chiller device
72: return unit
80: hose
90: mixed connector tube
100: bed
110: bio wear
650: movable body receiving portion
651: hot water path
653: Cold water flow path
655: mixed euro
656: through hole
671: 1st operation euro
673: 2nd movable flow path
675: 3rd operation euro
700: fixing bolt

Claims (7)

As a device constituting a bio three-dimensional printer, the bio material is temporarily received, and includes a dispenser unit configured to discharge the bio material downward through a nozzle and a water jacket coupled to the dispenser unit,
The dispenser portion includes a plate-shaped base plate;
A syringe fixing part fixed to the base plate and protruding in a direction perpendicular to the fixing surface, disposed in a cantilevered form to form a pair of pillars having a constant interval;
Includes a syringe that is elastically fixed in a form forcibly fitted to the syringe fixing part and is provided with a space for receiving biomaterial therein.
The water jacket is detachably coupled to the base plate, is arranged to surround the syringe in three dimensions, is provided with a water receiving space for receiving water therein, and an inlet for water inlet and an outlet for water outlet Dispenser unit water jacketing device for biomaterial constant temperature of the bio-three-dimensional printer. According to claim 1,
A plurality of engaging projections disposed along both sides of the base plate and protruding in a direction parallel to the syringe fixing part; And
It is provided on the water jacket; a plurality of coupling grooves formed concavely to slidably receive the coupling protrusion;
The water jacket is assembled so that the engaging groove portion is coupled to the engaging projection,
The water jacket is a dispenser portion water jacket device for the biomaterial constant temperature of the bio-three-dimensional printer, characterized in that sliding contact with the syringe fixing portion while in pressure contact with the side of the syringe fixing portion. According to claim 2,
The base plate is formed to extend in the longitudinal direction of the syringe from the syringe fixing portion and includes a syringe support portion for three-dimensionally supporting the back surface of the syringe,
Dispenser portion water jacket device for the biomaterial constant temperature of the bio three-dimensional printer, characterized in that the side of the syringe support and the water jacket is coupled to have an attraction by magnetic force. According to claim 1,
A chiller installed on the outside of the 3D bio printer, which accommodates the water flowing into the water jacket and is connected to the inlet and the outlet with flexible hoses, and each has a hot water storage tank and a cold water storage tank maintained at a constant temperature. Device; And
Dispenser portion water jacket device for the biomaterial constant temperature of the bio three-dimensional printer, characterized in that it comprises a mixing valve device for proportionally mixing the hot and cold water supplied from the chiller device. According to claim 4,
The mixing valve device includes a direct current motor;
A reduction gear coupled with the rotating shaft of the DC motor to increase torque and reduce rotation speed;
A sealing part disposed between the DC motor and the reduction gear to prevent the DC motor from being contaminated by moisture;
A fixed body installed to be fixed to the DC motor; And
Includes; movable body rotatably installed on the fixed body,
The fixed body is arranged to be spaced apart from the hot water flow path through which the hot water flows, and the hot water flow path along the rotational direction of the movable body, and the cold water flow path through which cold water flows and the mixed flow path disposed opposite the hot water flow path and the cold water flow path Equipped,
The hot water flow path and the extension line of the cold water flow path are formed to intersect at one point,
The movable body includes a first movable passage corresponding to the hot water passage, a second movable passage corresponding to the cold water passage, and a third movable passage corresponding to the mixed passage,
The first movable flow passage and the second movable flow passage join to form a third movable flow passage,
When the center line of the first movable flow path and the center line of the hot water flow path coincide with each other, the connection between the second movable flow path and the cold water flow path is completely blocked,
When the center line of the second movable channel and the center line of the cold water channel coincide with each other, the connection between the first movable channel and the hot water channel is completely blocked,
When the first movable passage and the hot water passage are partially communicated, the second movable passage and the cold water passage are also partially communicated, and the sum of the hot water and cold water flowing into the first movable passage and the second movable passage is always Constant,
The third movable flow path always communicates with the mixed flow path in a position where the first movable flow path or the second movable flow path communicates with the hot water flow path or the cold water flow path,
Wherein the first movable flow path and the second movable flow path is completely blocked from the hot water flow path and the cold water flow path, the third movable flow path is configured to be blocked from the mixed flow path Biomaterial constant temperature of the bio-three-dimensional printer Dispenser for water jacket device. The inlet of the water jacket and the water flowing into the water jacket to temporarily supply water to the water jacket coupled to the dispenser unit configured to temporarily discharge the biomaterial and discharge the biomaterial through a nozzle. An influent connection step of connecting the chiller device to be accommodated with a flexible hose through the mixing valve device;
A discharge water connection step of connecting the outlet of the water jacket and the chiller device with a hose; And
It includes; a temperature control step of controlling the temperature of the dispenser portion to a constant temperature by proportionally mixing the hot and cold water supplied from the chiller device in the mixing valve device to the inlet;
The mixing valve device,
A fixed body installed to be fixed to the DC motor; And
Includes; movable body rotatably installed on the fixed body,
The fixed body is arranged to be spaced apart from the hot water flow path through which the hot water flows, and the hot water flow path along the rotational direction of the movable body, and a cold water flow path through which cold water flows and a mixed flow path disposed opposite the hot water flow path and the cold water flow path. Equipped,
The hot water flow path and the extension line of the cold water flow path are formed to intersect at one point,
The movable body includes a first movable passage corresponding to the hot water passage, a second movable passage corresponding to the cold water passage, and a third movable passage corresponding to the mixed passage,
The first movable flow passage and the second movable flow passage join to form a third movable flow passage,
When the center line of the first movable flow path and the center line of the hot water flow path coincide with each other, the connection between the second movable flow path and the cold water flow path is completely blocked,
When the center line of the second movable channel and the center line of the cold water channel coincide with each other, the connection between the first movable channel and the hot water channel is completely blocked,
When the first movable passage and the hot water passage are partially communicated, the second movable passage and the cold water passage are also partially communicated, and the sum of hot water and cold water flowing into the first movable passage and the second movable passage is always Constant,
The third movable flow path always communicates with the mixed flow path in a position where the first movable flow path or the second movable flow path communicates with the hot water flow path or the cold water flow path,
Wherein the first movable flow path and the second movable flow path are completely blocked from the hot water flow path and the cold water flow path, and the third movable flow path is configured to be blocked from the mixed flow path. Multi-temperature control method of the water jacketing device for the dispenser. The inlet of the water jacket and the water flowing into the water jacket to temporarily supply water to the water jacket coupled to the dispenser unit configured to temporarily discharge the biomaterial and discharge the biomaterial through a nozzle. An influent connection step of connecting the chiller device to be accommodated with a flexible hose;
A discharge water connection step of connecting the outlet of the water jacket and the chiller device with a hose; And
Includes; a temperature control step of controlling the temperature of the dispenser portion to a constant temperature by mixing the hot and cold water supplied from the chiller device in a Y-shaped mixing connector tube and supplying it to the inlet.
The chiller device includes a hot water water pump for forcibly feeding hot water to the mixing connector tube, and a cold water water pump for forcibly forcing cold water to the mixing connector tube,
When the temperature of the water jacket is lower than a preset temperature, the hot water pump is operated,
When the temperature of the water jacket is higher than a preset temperature, the cold water pump is operated,
When the temperature of the water jacket is the same as the preset temperature, the hot water water pump and the cold water water pump are configured to operate at the same time. .

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