KR20200024056A - Specimen sliceing apparatus - Google Patents

Specimen sliceing apparatus Download PDF

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KR20200024056A
KR20200024056A KR1020180100810A KR20180100810A KR20200024056A KR 20200024056 A KR20200024056 A KR 20200024056A KR 1020180100810 A KR1020180100810 A KR 1020180100810A KR 20180100810 A KR20180100810 A KR 20180100810A KR 20200024056 A KR20200024056 A KR 20200024056A
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slide
sample
present
temperature
specimen
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KR1020180100810A
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Korean (ko)
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이미영
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(주)제노젠
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44708—Cooling
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00138—Slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00445—Other cooling arrangements

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Abstract

The present invention relates to a sample slide device including: a fan and a water tank (10) for controlling humidity; an open window (20) for injecting a cassette sample to be mounted on a slide glass; a hot plate (30) for controlling temperature and an intake device (40) for drying a slide; and a temperature and humidity sensor (50).

Description

검체 슬라이드 장치 {SPECIMEN SLICEING APPARATUS}Sample Slide Device {SPECIMEN SLICEING APPARATUS}

본 발명은 검체 슬라이드 장치에 관한 것으로, 더욱 상세하게는 정기영동 장치 사용 시 발생하는 열을 식혀주는 슬라이드 장치에 관한 것이다.The present invention relates to a specimen slide device, and more particularly, to a slide device to cool the heat generated when using the electrophoresis device.

전기영동장치는 DNA, RNA를 Plate에 넣으면 자동적이고 빠르게 분자량을 확인할 수 있는 분석기기이다. Electrophoresis device is an analyzer that can check molecular weight automatically and quickly when DNA and RNA are put on plate.

자석에는 서로 다른 극끼리 끌어당기는 힘 ‘인력’, 그리고 같은 극끼리 밀어내는 척력이 작용 하는데, 전기장도 인력과 척력을 가지고 있다.In magnets, the forces that attract each other's poles, the 'force', and the repulsive forces that push the same poles, have an attractive and repulsive electric field.

전기영동장치에서 물질들은 ‘아가로스 겔(해초 추출물을 용액에 녹여 만든 젤)’이란 매질을 타고 인력과 척력에 따라 음(-)전하를 띠는 분자는 +극 쪽으로, 양(+)전하를 띤 분자는 -극 쪽으로 이동하게 되는데, DNA는 음(-)전하를 띠는 물질이므로 +극 쪽으로 움직이게 되므로 단백질이나 DNA 같은 고분자 물질을 전기장을 띤 매질 안에서 이동시켜 분리하는 데 사용이 된다.In electrophoresis, the materials are taken through a medium called agarose gel (a gel made by dissolving seaweed extract in solution), and the negatively charged molecules are positively charged and positively charged according to attraction and repulsion. Molecular molecules move toward the pole, and because DNA is a negative-charged substance, they move toward the positive pole, which is used to separate high-molecular substances such as proteins and DNA by moving them in an electric medium.

단백질이나 DNA같은 고분자 물질을 전기 영동장치로 이송을 시키고자 할 경우 슬라이드 장치를 사용하는데 이를 이동 시키기 위하여 카세트에 검체를 올려 놓고 전류를 인가하면 검체가 이동을 하게 되는데, 일정한 온도 이상이 될 경우 검체의 속성이 변하여 정확한 분석이 어려운 점이 있다. In order to transfer high molecular substance such as protein or DNA to electrophoresis device, slide device is used.In order to move it, the sample moves when the sample is placed on the cassette and the current is applied. Because of the changed properties, it is difficult to accurately analyze.

또한 전류가 안정적으로 흘러야 하는데 이러한 전류의 불안정은 검체의 속성을 변화시켜 정확한 분석 하지 못하는 경우도 발생을 한다. In addition, the current must flow stably, and the instability of the current can change the properties of the sample and cause inaccurate analysis.

대한민국 공개특허공보 제 10-2016-0006112 (공개일자: 2016년01월18일, 발명의 명칭: 전기영동을 이용한 조직 투명화 장치, 출원인: 로고스바이오시스템스)Republic of Korea Patent Publication No. 10-2016-0006112 (published date: January 18, 2016, the name of the invention: tissue transparent device using electrophoresis, Applicant: Logos Bio Systems)

상기와 같은 문제점을 해결하기 위한 본 발명의 목적은 전기영동장치의 열을 시켜주는 냉각시스템을 적용하여 검체가 온도의 영향을 받지 않도록 하고 잔정적인 전류가 흐를 수 있도록 하는 검체 슬라이드를 제공하는 데 있다.An object of the present invention for solving the above problems is to provide a sample slide to apply a cooling system that heats the electrophoretic device so that the sample is not affected by the temperature and the residual current flows. .

상기 목적을 달성하기 위한 본 발명의 검체 슬라이드 장치는 습도조절을 위한 팬 및 수조(10), 슬라이드 글래스 장착을 위한 카세트 시료를 주입하기 위한 오픈 창(20), 온도 조절을 하기 위한 열판(30), 슬라이드 건조를 위한 흡기 장치(40), 및 온습도 센서(50)를 제공한다.Specimen slide device of the present invention for achieving the above object is a fan and a tank (10) for humidity control, an open window (20) for injecting a cassette sample for mounting slide glass, a hot plate (30) for temperature control , An intake apparatus 40 for slide drying, and a temperature and humidity sensor 50 are provided.

이러한 특징에 따르면, 본 발명은 전기영동기 내로 검체가 안정적으로 공급 될 수 있도록 할 수 있다.According to this feature, the present invention can enable the sample to be stably supplied into the electrophoresis device.

도 1은 본 발명의 일 실시예에 따른 검체 슬라이드장치의 구성도이다.1 is a block diagram of a specimen slide device according to an embodiment of the present invention.

아래에서는 첨부한 도면을 참고로 하여 본 발명의 실시예에 대하여 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 상세히 설명한다. 그러나 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며 여기에서 설명하는 실시예에 한정되지 않는다. 그리고 도면에서 본 발명을 명확하게 설명하기 위해서 설명과 관계없는 부분은 생략하였으며, 명세서 전체를 통하여 유사한 부분에 대해서는 유사한 도면 부호를 붙였다.DETAILED DESCRIPTION Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art may easily implement the present invention. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention. In the drawings, parts irrelevant to the description are omitted in order to clearly describe the present invention, and like reference numerals designate like parts throughout the specification.

그러면 첨부한 도면을 참고로 하여 본 발명의 일 실시예에 따른 검체 슬라이드 장치에 대하여 설명한다.Next, a specimen slide device according to an embodiment of the present invention will be described with reference to the accompanying drawings.

도 1을 참조하면, 본 발명의 일 실시예에 따른 검체 슬라이드 장치는 상기 목적을 달성하기 위한 본 발명의 검체 슬라이드 장치는 습도조절을 위한 팬 및 수조(10), 슬라이드 글래스 장착을 위한 카세트 시료를 주입하기 위한 오픈 창(20), 온도 조절을 하기 위한 열판(30), 슬라이드 건조를 위한 흡기 장치(40), 및 온습도 센서(50)를 포함하여 구비되어 있다.Referring to Figure 1, the specimen slide device according to an embodiment of the present invention, the specimen slide device of the present invention for achieving the above object is a cassette and a sample for mounting the fan and the tank (10), slide glass for humidity control An open window 20 for injection, a hot plate 30 for temperature control, an intake device 40 for slide drying, and a temperature and humidity sensor 50 are provided.

본 발명의 검체 슬라이드 장치의 작동을 DNA를 예로들어 설명하면, 카세트에 슬라이드를 장착시키고 온도 조절을 위한 열판에 전류를 인가하면 DNA는 음(-)전하를 띠는 물질 이므로 양(+)전하 쪽으로 이동을 하며 전기 영동장치에 장착이 된다.Referring to the operation of the sample slide device of the present invention using DNA as an example, when the slide is mounted on a cassette and a current is applied to a hot plate for temperature control, the DNA is a negative charge material, so it is directed toward the positive charge side. It is moved and mounted on the electrophoresis device.

이때, 온습도 센서(50)에 의해 온도와 습도를 측정하여 적절한 상태를 유지하기 위하여 열판에 전류를 인가한다. At this time, the temperature and humidity are measured by the temperature and humidity sensor 50 to apply a current to the hot plate in order to maintain an appropriate state.

만약 온도와 습도가 설정된 값보다 올라갈 경우에는 전류의 인가를 중지하여 온도와 습도를 조절한다.If the temperature and humidity rise above the set value, stop the application of current and adjust the temperature and humidity.

전기 영동장치가 동작을 완료하면 흡기장치(40)가 작동되어 검체의 표명에 있는 습기를 빠르게 제거 한다.When the electrophoresis device completes the operation, the intake device 40 is operated to quickly remove moisture in the sample's manifestation.

이렇게 함으로써 검체 슬라이드가 안정적으로 동작 할 수 있도록 해준다.This ensures that the specimen slides are stable.

이상에서 본 발명의 실시예에 대하여 상세하게 설명하였지만 본 발명의 권리범위는 이에 한정되는 것은 아니고 다음의 청구범위에서 정의하고 있는 본 발명의 기본 개념을 이용한 당업자의 여러 변형 및 개량 형태 또한 본 발명의 권리범위에 속하는 것이다.Although the embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concepts of the present invention defined in the following claims are also provided. It belongs to the scope of rights.

10: 팬 및 수조 20: 오픈창
30: 온도조절 열판 40: 슬라이드 건조를 위한 흡기장치
50: 온습도 센서10: fan and tank 20: open window
30: temperature control hot plate 40: intake device for drying the slide
50: temperature and humidity sensor

Claims (1)

습도조절을 위한 팬 및 수조(10),
슬라이드 글래스 장착을 위한 카세트 시료를 주입하기 위한 오픈 창(20),
온도 조절을 하기 위한 열판(30),
슬라이드 건조를 위한 흡기 장치(40), 및 온습도 센서(50)
를 포함하는 검체 슬라이드장치.Fan and bath (10) for controlling humidity,
Open window 20 for injecting a cassette sample for mounting slide glass,
Hot plate 30 for temperature control,
Intake apparatus 40 for slide drying, and temperature and humidity sensor 50
Specimen slide device comprising a.
KR1020180100810A 2018-08-27 2018-08-27 Specimen sliceing apparatus KR20200024056A (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160006112A (en) 2014-07-07 2016-01-18 (주)로고스바이오시스템스 Apparatus for Electrophoretic Tissue Clearing

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160006112A (en) 2014-07-07 2016-01-18 (주)로고스바이오시스템스 Apparatus for Electrophoretic Tissue Clearing

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