KR20200009385A - Acaricidal composition against Dermanyssus gallinae comprising caprylic acid - Google Patents

Abstract

The present invention relates to a Dermanyssus gallinae insecticidal composition comprising caprylic acid or a salt thereof and an insecticidal method of Dermanyssus gallinae using same. The insecticidal composition of the present invention has a high fertilizing effect on Dermanyssus gallinae, and is environmentally friendly and safe for the human body, thereby being usefully used as a poultry Dermanyssus gallinae insecticide.

Description

Acaricidal composition against Dermanyssus gallinae comprising caprylic acid}

The present invention relates to a composition for killing chicken ticks containing caprylic acid or salts thereof and a method for killing chicken ticks using the same.

Chicken ticks are a globally distributed external parasite that cause significant economic losses in poultry farms. This species is a bloodsucking parasite that lives on chickens and wild birds and is a bloodsucking parasite that should thrive in poultry farms, which can thrive in poultry and lead to anemia, reduced productivity and even death in laying hens.

It is also a mediator of poultry avian spirochetes, chickenpox virus, Newcastle virus, Chubaekri, poultrypus and cholera. In their dense houses, they can disgust people. The stages of vampires during their life cycles are usually worms and females. In very rare cases, males also do vampires but do not vampires.

As such, they have relied primarily on chemical control to control the most threatening people in poultry farms. In particular, insecticides such as fipronil and bifenthrin, which may adversely affect the human body, have been used. Recently, as insecticide egg waves and the like occur, the risk of the insecticides has been raised. Accordingly, since the insecticide can not be continuously sprayed directly to the poultry, this affects the productivity of farmers, etc., the residue of the pesticides, such as eggs, chicken, etc. are likely to cause great harm to the human body.

Therefore, in order to catch chicken mites inhabiting poultry, there is a need for the development of a safe and environmentally friendly material that is not harmful to the human body without residual pesticides.

Under these backgrounds, the present inventors have made an effort to develop an environment-friendly and safe human body material while having a high astringent effect on chicken ticks, and thus, by confirming that caprylic acid has a high astringent effect on chicken ticks, the present invention has been completed. It was.

(Patent Document 1) KR10-2018-0021417

One object of the present invention is to provide a composition for killing chicken ticks.

Still another object of the present invention is to provide a chicken tick insecticide method.

This will be described in detail below. In addition, each description and embodiment disclosed in this application may apply to each other description and embodiment. That is, all combinations of the various elements disclosed in this application are within the scope of the present application. In addition, the scope of the present application is not to be limited by the specific description described below.

One aspect of the present invention for achieving the above object comprises a caprylic acid (caprylic acid) or a salt thereof as an active ingredient, chicken tick ( Dermanyssus gallinae ) relates to a pesticidal composition.

Caprylic acid is a compound having the structure of Formula 1 below and is a saturated fatty acid composed of eight carbons.

In the present invention, it is based on the first finding that caprylic acid has a mite effect on chicken mites, in particular, belongs to the medium chain fatty acid group, such as caprylic acid, capric acid having 10 carbons in the medium chain fatty acid, Unlike lauric acid having 12 carbons, caprylic acid having 8 carbons is characterized by a markedly high mite effect on chicken mites.

The salt of caprylic acid in the present invention means a salt that can be used veterinarily among salts in which cations and anions are bound by electrostatic attraction, and have a relatively nontoxic and harmless effect on an individual. By concentration, the side effects attributable to this salt mean any organic or inorganic addition salt that does not degrade the beneficial efficacy of the caprylic acid. Such salts include acid addition salts formed by pharmaceutically or veterinary acceptable free acids or metal salts formed by bases.

Organic acids and inorganic acids may be used as the free acid, hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, tartaric acid, etc. may be used as the inorganic acid, and methanesulfonic acid, p-toluenesulfonic acid, acetic acid, trifluoroacetic acid, maleic acid ( maleic acid), succinic acid, oxalic acid, benzoic acid, tartaric acid, fumaric acid, manderic acid, propionic acid, citric acid, lactic acid, glycolic acid, gluconic acid ( gluconic acid, galacturonic acid, glutamic acid, glutaric acid, glucuronic acid, glucuronic acid, aspartic acid, ascorbic acid, carbonic acid, vanic acid, hydroiodic acid, etc. This is not restrictive.

Bases can also be used to make pharmaceutically or veterinary acceptable metal salts. Alkali metal salts or alkaline earth metal salts are obtained, for example, by dissolving a compound in an excess of alkali metal hydroxide or alkaline earth metal hydroxide solution, filtering the insoluble compound salt, and then evaporating and drying the filtrate. In this case, as the metal salt, it is particularly suitable to prepare sodium, potassium, or calcium salt in a pharmaceutical or food form, but is not limited thereto. Corresponding silver salts can also be obtained by reacting an alkali or alkaline earth metal salt with a suitable silver salt (eg, silver nitrate).

Pharmaceutical or veterinary acceptable salts of caprylic acid of the invention include salts of acidic or basic groups which may be present in the caprylic acid, unless otherwise specified. For example, pharmaceutically or veterinary acceptable salts may include sodium, calcium and potassium salts of the hydroxy group, and other pharmaceutically or veterinary acceptable salts of the amino group include hydrobromide, sulfate, hydrogen Sulfate, phosphate, hydrogen phosphate, dihydrogen phosphate, acetate, succinate, citrate, tartrate, lactate, mandelate, methanesulfonate (mesylate) and p-toluenesulfonate (tosylate) salts; It may be prepared through a method for preparing a salt known in the art.

The insecticidal composition of the present invention has acaricide and repellent effect on chicken ticks. Chicken mite ( Dermanyssus gallinae ) is an external parasite belonging to the mite family of mite and mid-middle mollusks. In the present invention, the chicken mite may also be called wagumo. Chicken ticks, unlike other ticks, tend to be vampire-blooded because they are parasitic in chickens at night and are hidden in shadows and crevices during the day, making them difficult to detect. many. In addition, there are differences in many aspects of life in terms of long-term survival without a host. In addition, young mites after hatching are about 0.5 mm long and have 3 pairs of legs. Adult ticks are 4 pairs of legs 1 mm long. Adult ticks may be more than 1 mm in the blood-sucking stage immediately after vampires, resulting in a black-red appearance by blood. Vampires per animal are very small, but in large farms, chickens die of anemia. Chicken mites become larva mites after hatching from eggs and become the first young mites without vampire processes. The first young mite becomes a second young mite within 24 hours after vampire, and then further vampires become a mature mite. About nine days in the egg becomes a mature mite, repeating vampires and spawning.

In the present invention, it is characterized by providing a composition having insecticidal, acaricide, and acaricide efficacy against chicken ticks, which are completely different from other ticks, in terms of life, and survival patterns. Even if they belong to the same tick family, the insecticidal and acaricide efficacy may be completely different depending on the way of life, lifestyle, and parasitic species. In addition, as a result of confirming the astringent effect on chicken mites using a conventional plant extract known to have an insecticidal effect on ticks, only a mild acaricide effect was observed for chicken mites only in menthol and mifudo, and in other plant extracts There was no acaricide effect for (Example 4). In addition, using the acetic acid, formic acid, fatty acids, known to be a pesticidal effect, capric acid, lauric acid belonging to the same heavy chain fatty acid group, and confirmed that the fertilizing effect on chicken ticks, the fertilization effect was not seen or very insignificant Only, it was confirmed that only caprylic acid has the effect of killing chicken mite 100% within 10 minutes (Example 3).

From these results, it can be seen that chicken mites have different insecticidal mechanisms and insecticidal effects than other mites and pests, and even that substances known to be effective against other mites cannot be said to have a killing effect on chicken mites. Could.

"Insecticide" in the present invention means the use to kill chicken ticks or to avoid chicken ticks. Insecticidal subjects in the present invention include chicken ticks, larvae, adults. Insecticidal in the present invention is used in the same manner as acaricide mite, acaricide efficacy.

The composition containing caprylic acid of the present invention can be utilized as an active ingredient of a veterinary composition having a tick effect on chicken ticks.

The insecticidal composition of the present invention may further include an additive such as a veterinary acceptable carrier or excipient. Insecticidal compositions of the present invention may be mixed with conventional solid carriers, liquid carriers, gas carriers or baits, in addition to caprylic acid or salts thereof, or absorbed into basic materials such as porous ceramic plates or nonwoven fabrics, followed by surfactants and If necessary, other auxiliaries may be added to prepare them in various forms such as oil sprays, mats, coils, emulsifiable concentrates, powders, wetting powders, flows, microcapsules, oily preparations, granules, dusts, aerosols, smokers ( (Eg, fogging), vaporizable formulations, smoking formulations, toxic attractants, anti-mite sheets, and resin formulations.

Solid carriers that can be used in the pesticides of the present invention include fine powders or granules of clay materials such as kaolin clay, diatomaceous earth, synthetic hydrated silicon oxide, bentonite, fuvasami clay and acid clay; Various talc, ceramic and other inorganic materials such as biotite, quartz, sulfur, activated carbon, calcium carbonate and hydrated silica; And chemical fertilizers such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea and ammonium chloride.

Liquid carriers include water; Alcohols such as methanol and ethanol; Ketones such as acetone and methyl ethyl ketone; Aromatic hydrocarbons such as benzene, toluene, xylene, ethylbenzene and methylnaphthalene; Aliphatic hydrocarbons such as hexane, cyclohexane, kerosine and light oils; Esters such as ethyl acetate and butyl acetate; Nitriles such as acetonitrile and isobutyronitrile; Ethers such as diisopropylether and dioxane; Acid amides such as N, N-dimethylformamide and N, N-dimethylacetamide; Halogenated hydrocarbons such as dichloromethane, trichloroethane and carbon tetrachloride; Dimethyl sulfoxide; And vegetable oils such as soybean oil and cottonseed oil.

Gas carriers or propellants may include freon gas, butane gas, LPG (liquefied petroleum gas), dimethyl ether, and carbon dioxide.

Base materials for use in toxic handouts include, but are not limited to, handout materials such as grain powder, vegetable oils, sugars and crystalline cellulose; Antioxidants such as dibutylhydroxytoluene and nordihydroguaiaric acid; Preservatives such as dehydroacetic acid; Materials for preventing non-eating and drinking, such as pepper powder; And attractant flavors such as cheese and onion flavors.

Adjuvants such as adhesives or dispersants include casein, gelatin; Polysaccharides such as starch, gum arabic, cellulose derivatives and alginic acid; Lignin derivatives, bentonite, sugars and synthetic water soluble polymers such as polyvinyl alcohol, polyvinyl pyrrolidone and polyacrylic acid.

Stabilizers include PAT (isopropyl acid phosphate), BHT (2,6-di-tert-butyl-4-methylphenol), BHA (2-tert-butyl-4-methoxyphenol and 3-tert-butyl- Mixtures of 4-methoxyphenol), vegetable oils, mineral oils, surfactants, fatty acids and esters thereof.

The composition of the present invention may further include a surfactant for mixing the aqueous layer and the oil layer. The surfactant may be one or more selected from the group consisting of ethyl alcohol, sodium lauroyl sarcosinate, elpan 84 / SCI / sodium cocoyl isethionate, and lauryl glucoside.

The insecticidal composition of the present invention may contain 0.001 to 99.9% (v / v) of caprylic acid or a salt thereof, specifically, 0.001 to 80%, more specifically 0.001 to 50%, even more specifically 0.01 to It may include 30% by weight, but is not limited thereto.

In addition, the pesticidal composition of the present invention further includes capric acid or a salt thereof, lauric acid or a salt thereof, or a combination thereof as an additive.

The veterinary composition of the present invention can be applied to the poultry subject to the permissible amount, can be applied by spraying, such as application, spraying, the dosage and administration according to specific issues, such as the degree of infection of chicken ticks, the degree of relief The number of times can be adjusted and applied. Specifically, the concentration of the active ingredient in the poultry breeding area may be sprayed at a concentration of 5000 ppm or less, sprayed at a concentration of 2000 ppm or less, sprayed at a concentration of 1000 ppm, concentration of 500 ppm or less Can be sprayed to

However, the spread amount may vary depending on the amount of the active ingredient included in the veterinary composition, the condition of the individual (weight, etc.), the severity of the chicken tick infection state. If necessary, the total daily dose may be divided into several times and divided into several times during the day.

Another aspect of the present invention relates to a method for killing insects, the tick comprising the step of spraying the insecticidal composition on poultry, feed, drinking water or poultry barn.

Detailed description of the insecticidal composition is the same as described above.

The insecticidal method of the present invention includes the application of the insecticidal composition of the present invention in consideration of various factors such as the formulation of the pesticide, the object of the insecticide, the place and the method of insecticide, and in one preferred embodiment the chicken tick in an effective amount Or processing in the habitat.

The use method is preferably a method such as spray treatment, sleep treatment, contact treatment, etc., the application dose can be used as a dose to apply a conventional insecticide.

The insecticidal composition of the present invention is an environmentally friendly and safe human body material while having a high killing effect on chicken mites, and can be usefully used as a chicken mite insecticide of poultry.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited only to these examples.

Example  1: caprylic acid Wagumo A mite  And evasion experiments

Caprylic acid (origin: UK, purchased from Chemkose Co. Ltd) has been tested for the effectiveness of mites and repellents against chicken mites ( Dermanyssus gallinae , Wagumo ) [Journal of Biomedical Research 10 (1): 23-27, It was measured by referring to the experimental method described in 2009]. Specifically, first, a line was drawn at the center diameter on the circular filter paper, and a straight line was drawn at a point 5 mm apart from both sides of the center line. This line divides the filter paper into four zones, designated Zone 1, Zone 2, Zone 3, and Zone 4 from the left zone.

40 [mu] l of the above sample was evenly sprinkled with a micropipette in zones 3 and 4, which are semicircles centered on the center line. Then, house dust mites and chicken mites were dispensed three times along the center line on each filter paper at regular intervals, wherein the number of aliquots was about 20 to 40 per aliquot.

The killing rate and avoidance rate of chicken ticks were checked at 1 hour, 2 hours and 3 hours after dispensing, and the two outward zones, Zone 1 and Zone 4, divided by the lines drawn on the filter paper. Count the number of chicken ticks and record the number of each. In addition, the caprylic acid content was 1 to 8% (vol%), and the hourly mite rate and the avoidance rate of each content were calculated by the following equations (1) and (2).

The result of confirming the fat mite rate (%) for chicken mite of caprylic acid was as Table 1 below.

content 0% One% 2% 4% 8% 3 hours 14.3 50.0 73.1 91.3 100.0 24 hours 19.0 68.2 84.6 100.0 100.0

In addition, the repellent activity (%) of the caprylic acid chicken tick is shown in Table 2 below.

content 0% One% 2% 4% 8% 1 hours 14.3% 45.5% 80.7% 100% 100% 2 hours 19.0% 50.0% 84.6% 100% 100% 3 hours 19.0% 45.5% 84.6% 100% 100%

As confirmed in Table 2, caprylic acid has a mite effect within 3 hours for chicken mites even if it contains only 1%, showed more than 91% of the mite activity within 3 hours at 4% content, 24 hours The rate of fat mites was within 100%. In addition, in relation to the repellent activity, caprylic acid contained 80% or more of repellent activity within 1 hour with respect to chicken ticks, even when included in only 2% content, and exhibited 100% repellent activity when included in 4% content.

Through the above results, it was confirmed that caprylic acid significantly increased the activity of the mite and the repellent specifically for chicken ticks.

Example  2: concentration of caprylic acid A mite  Experiment

Samples diluted in water by caprylic acid concentration were sprayed onto an incu tissue for insect experiments, waited for the water to dry, and then the time taken for killing the chicken tick was 100%. The results were as shown in Table 3 below.

Dilution ratio with water 1:10 1:20 1:40 1:60 1:80 Until 100%
Time taken Immediately Immediately 2 minutes 18 minutes 100% die after 24 hours

Based on the above results, when caprylic acid was diluted from water 1:10 (caprylic acid: water volume ratio) to 1:20, it was confirmed that 100% killing of chicken ticks was immediate and 1:40 to 1: When diluted to 60, it was confirmed that the chicken tick can be killed 100% within 18 minutes.

Example  3: Chicken tick  About Fatty acid  Efficacy test

The effectiveness of the mite on chicken mites was confirmed by the type of fatty acid. Specifically, after dissolving each fatty acid in ethanol, diluted 1:50 in water, and then sprayed several fatty acids on the incu tissue for insect experiment (waiting for the water to dry), then put chicken mite 100 The time taken to kill% was measured and survival rate was confirmed if 100% was not killed by 24 hours. Experimental results were as shown in Table 4 below.

Fatty acid types Time to 100% Death Survival rate after 24 hours (*) Acetic acid - over 90(**) Formic acid - 100% Butyric acid - over 90 Caproic acid - over 90 Caprylic acid Within 10 minutes 0% Capric acid - 50-55% Lauric acid - over 90 Palmitic acid - 80% or more Oleic acid - 20-30%

* About 100 ~ 300 chicken mites entered randomly by incu tissue, and the higher the survival rate, the more active the movement, so that the correct counting was not possible. Calculated and calculated.

** More than 90% is alive in certain compartments, but only a few individuals are found dead in other places.

Even fatty acids with similar chemical structure showed a significant difference in the rate of mites for chicken ticks. In addition, even if it is included in the same heavy chain fatty acid group, it was confirmed that caprylic acid, a specific fatty acid, showed an overwhelming ability to kill ticks against chicken mites, compared to capric acid and lauric acid belonging to other heavy chain fatty acids. These results are due to the difference between chicken mites and other ticks, only caprylic acid is interpreted to be significantly higher than the mites for chicken mites.

Example  4: Salbi  Comparison test for each plant extract having an effect

Plant extracts known to have an astringent effect on ticks were prepared, and the acaricide effects on caprylic acid and chicken ticks of the present invention were compared. The results are shown in Table 5 below.

Substance (1:10) 100% of application time Caprylic acid 1 minute Thistle no effect Mifudo 10% after 24 hours Donkey no effect Bay leaf no effect Neck skin no effect Gosam no effect Eoseongcho no effect Droopy no effect Cat leaves no effect Lemongrass no effect Peppermint no effect Menthol 20% after 24 hours lavender no effect cinnamon no effect Ylang-ylang no effect Lime no effect Rosemary no effect Eucalyptus no effect Lemon balm no effect geranium no effect Spearmint no effect Allicin no effect

As a result of applying the natural extracts having the astringent effect of Table 5 to chicken mite agar, it was confirmed that the extract from the distilled water at a 1:10 ratio has a totally different astringent effect on the chicken mite.

The menthol extract and mifudo extract were confirmed to show a 10-20% acaricide effect on chicken mites after 24 hours of application, and the remaining extracts were found to have no acaricide effect on chicken mites. On the other hand, caprylic acid of the present invention was confirmed to have a superior chicken tick killing effect compared to other natural extracts known to have a conventional acaricide effect against ticks.

The natural extract known to have an effect on the mite is a result of the fertilization effect is lowered on the chicken mite, even if there is a fertilization effect on other types of ticks can not necessarily be said to have a fertilizing effect on the chicken mite, depending on the type of tick It could be confirmed that the killing effect is unpredictable.

Example  5: of caprylic acid according to the type of surfactant Chicken tick  About Efficiency  Comparative experiment

In order to prepare a tick for chicken mite using caprylic acid, various kinds of surfactants were mixed with caprylic acid and diluted 1:20, and thus the degree of diffusion of caprylic acid and A comparison of the effect of acaricide was carried out. The results were as shown in Table 6 below.

Type of surfactant Diffusion Degree of Caprylic Acid Astringent effect Apple wash (sodium cocoyl apple amino acids) / country of origin: France / manufacturer: SEPPIC good no effect Cocobetaine (LPB) Lauramidopropylbetaine good no effect ELPAN 84 / SCI / Sodium Cocoil Isethionate good 95% PCG (potassium cocoyl glycinate) good no effect Decyl glucoside koncopa good no effect Lauryl glucoside / alkyl polyglucoside good 100% Disodium laureth sulfosuccinate good no effect Potassium Cocoate Potassium Cocoate good no effect Babasu Betaine (Babasuamidopropylbetaine) Natural Surfactants / Amphoteric Surfactants good no effect Olive surfactant good no effect Coco betaine les good no effect Sodium Lauroyl Sarcosinate good 90% Ethyl Alcohol 83% good 100% Ethyl alcohol 99.9% good 100%

After mixing the same ratio of different surfactants, it was confirmed that the astringent effect on chicken mites was significantly reduced in some substances, ethyl alcohol, sodium lauroyl sarcosinate, elpan 84 / SCI among surfactants. It was confirmed that the diffusion of caprylic acid in sodium cocoyl isethionate and lauryl glucoside was stable and exhibited the best acaricide effect.

Through the above results, depending on the type of the surfactant may affect the acaricidal effect of caprylic acid on chicken ticks, while maintaining the acaricide effect on chicken ticks and at the same time with a specific surfactant to help the diffusion of caprylic acid The combination was important.

From the above description, those skilled in the art will understand that the present application can be implemented in other specific forms without changing the technical spirit or essential features. In this regard, it should be understood that the embodiments described above are exemplary in all respects and not limiting. The scope of the present application should be construed that all changes or modifications derived from the meaning and scope of the following claims and equivalent concepts rather than the detailed description are included in the scope of the present application.

Claims (7)

Caprylic acid (caprylic acid) or a salt thereof as an active ingredient, chicken tick ( Dermanyssus gallinae ) insecticidal composition.
The insecticidal composition according to claim 1, wherein the caprylic acid or a salt thereof is included in an amount of 0.001 to 50% (v / v).
The insecticidal composition according to claim 1, further comprising a surfactant.
4. The insecticidal composition according to claim 3, wherein the surfactant is at least one selected from the group consisting of ethyl alcohol, sodium lauroyl sarcosinate, elpan 84 / SCI / sodium cocoyl isethionate, and lauryl glucoside.
The insecticidal composition according to claim 1, further comprising capric acid or a salt thereof, lauric acid or a salt thereof, or a combination thereof.
The formulation of claim 1 wherein the oil spray, mat, coil, emulsifiable concentrate, powder, wetting powder, fluid, microcapsule, oily formulation, granule, dust, aerosol, smoker, vaporizable formulation, smoking formulation Insecticide composition comprising any one selected from the group consisting of toxic handouts, mite-proof sheets and resin formulations.
The insecticidal method of claim 1, comprising spraying the insecticide composition in poultry, feed, drinking water or poultry barn.