KR20200008456A - Composition for seed enhancement comprising cellulose or cellulose derivatives and water and method for treating seed enhancement by using the same - Google Patents

Abstract

The present invention relates to a treatment method for enhancing vitality of seeds and to a composition for treating seed vitality used in the treatment method and, more specifically, to a solid priming matrix for seed vitality enhancement, which comprises a mixture of a cellulose having crystallinity of greater than 60 to maintain adequate moisture potential or a derivative thereof and a cellulose having the crystallinity of 45 or less or a derivative thereof, to a composition comprising the same and to a seed vitality treatment method using the same, wherein the seeds treated with the solid priming matrix, the composition and the method exhibit excellent germination rate.

Description

Composition for seed enhancement comprising cellulose or cellulose derivatives and water and method for treating seed enhancement by using the same}

The present invention relates to a treatment method for enhancing seed vitality and a composition for treating seed vitality used in the treatment method, and more particularly, as a solid priming matrix for seed vitality treatment. It relates to a solid priming matrix comprising a combination of cellulose having a degree of crystallinity or a derivative thereof, a composition for treating seed vitality comprising the same, and a method for treating seed vitality using the same.

Seed enhancement technology refers to a treatment for improving the vitality of a seed after harvesting and before seeding. Seed germination requires moisture, oxygen and adequate temperature. The water absorption process of seeds is divided into rapid absorption stage (1 stage), post-absorption stage (2 stage), and absorption of moisture again with germination of seedlings (3 stage). There is, but in three stages there is no drying resistance. There are two ways to moisturize the seed. One method is to immerse the seed in water, which is non-controlled water uptake which allows moisture to be freely absorbed by the seed. Seeds absorb water indefinitely, so dormant live seeds eventually germinate when the right temperature and oxygen are supplied. To stop the hydration that the seeds can go to the beginning of stage 3, the absorption process must be stopped within a certain period of time. Moisture control absorption treatment is a treatment to control the water potential (see the water potential) of the seed to prevent absorption until the germination stage (absorption stage 3).

Recently, as a method of increasing seed vitality, solid matrix priming (SMP) moisture control absorption is applied. This is to adjust the water absorption of the seeds using the osmotic principle, a method of treating by mixing the seeds with a low water potential by adding a little water to solid powders such as vermiculite, lignite, diatomaceous earth. Priming with the solution requires continuous supply of air to the solution. Seeds can be harmed in inorganic salt solutions. SMP treatment, on the other hand, does not have to be supplied with air and has the advantage of maintaining a moderate moisture potential.

However, in the past, vermiculite was used as a solid priming material, but due to low moisture content, the solid priming effect was not high and Microcel-E (Calcium Silicate, diatomaceous earth) had a high dust generation rate, which made it difficult to apply to seed treatment processes. . Furthermore, there is a drawback that the high concentration of ions in the treatment agent may adversely affect the germination.

Because of the problems with the conventional solid priming techniques as described above, there has been a need for improved seed priming matrices for seed vitality treatment and seed vitality treatment methods for maintaining proper moisture potential and uniform germination of seeds.

[Patent Document 1] Korean Patent Publication No. 10-2006-0017498 [Patent Document 2] Korean Patent Publication No. 10-2016-0108483 [Patent Document 3] Korean Patent Publication No. 10-2016-7033401 [Patent Document 4] Korean Patent Publication No. 10-2017-7013865

The present invention has been made in accordance with the above requirements, by combining a cellulose having a hydrophilic group as a solid priming matrix having a high water content and a specific crystallinity, to improve the vitality of the seed to increase seed vitality treatment composition and It is an object to provide a method for increasing seed vitality.

As one aspect for achieving the above object, the present invention relates to a solid priming matrix comprising a mixture of cellulose having a crystallinity of greater than 60 or a derivative thereof and cellulose having a crystallinity of 45 or less.

The present invention also relates to a composition for seed vitality enhancing treatment comprising the solid priming matrix.

In addition, the present invention,

(a) mixing the composition for seed vitality treatment comprising seed before seeding and water with said solid priming matrix and less than 5% by weight inorganic salts; And

(b) washing the mixture stored in step (a) to obtain seed and drying the seed.

When conventional vermiculite or inorganic powder having a low water content is applied as a solid priming matrix, it is difficult to maintain proper moisture potential of the seed, and thus there is a disadvantage that it is not uniform germination. However, the present invention applies a combination of cellulose or derivatives thereof having a hydrophilic group, a crystallinity of more than 60, and cellulose or derivatives thereof of 45 or less, and a composition for treating seed vitality including water and seeds containing inorganic salts below a specific content. It is possible to provide a uniform and high germination rate of seeds by maintaining a constant moisture potential required for seed germination by providing a.

1 is a diagram showing the chemical structure of cellulose.
2 to 4 are graphs showing the results of X-ray diffraction analysis of cellulose used in Examples and Comparative Examples.

The terminology used herein is for the purpose of describing exemplary embodiments only and is not intended to be limiting of the invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. As used herein, the terms "comprise", "comprise" or "have" are intended to indicate that there is a feature, step, component, or combination thereof that is practiced, and that one or more other features or steps, It should be understood that it does not exclude in advance the possibility of the presence or addition of components, or combinations thereof.

As the invention allows for various changes and numerous embodiments, particular embodiments will be illustrated and described in detail below. However, this is not intended to limit the present invention to the specific disclosed form, it should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present invention.

Hereinafter, a method for seed vitality improving treatment composition and a method for improving seed vitality using the same according to a specific embodiment of the present invention will be described in detail.

In the present invention, "seed vitality" may mean to enhance the germination rate and / or germination rate of the seed to be treated.

The solid priming matrix according to the invention may comprise a combination of two or more of cellulose or cellulose derivatives having different specific ranges of crystallinity. The term "cellulose" is an environmentally friendly material as the main constituent of the plant cell wall, and may have a structure as shown in FIG. 1. According to the source of cellulose, the crystal state, the degree of crystallinity, etc. vary, and the characteristics of the cellulose are different depending on the degree of crystallinity. Cellulose has a variety of crystal forms, cellulose according to the present invention may have a cellulose I form which is a form present in natural cellulose. The crystallinity index (CI) region refers to the degree of crystallization of any substance, and is measured by a method such as X-ray diffraction (XRD diffraction), and in the present invention, XRD measured by X-ray diffraction It means the relative size of the sum of the regions (I_crystal) and the sum of the amorphous regions (I_amorphous) of the crystalline peaks in the pattern, which is represented by Equation 1 below.

[Equation 1]

Crystallinity (%) = 100 * I_crystal / (I_crystal + I_amorphous)

After subtracting the background signal measured without cellulose, the mean value is calculated from the height ratio between the overall peak intensity and the peak intensity of the crystal peak.

Specifically, the present invention includes a mixture of a cellulose having a crystallinity of greater than 60 or a derivative thereof and a cellulose having a crystallinity of 45 or less or a derivative thereof. The cellulose may be included in two or more kinds in the mixture.

The cellulose having a crystallinity of more than 60 or a derivative thereof may be used without limitation as long as it satisfies the crystallinity range, for example, PH-101 having a crystallinity of greater than 65, PH-105 having a crystallinity of 60 to 65 It may be a cellulose having a code name

In addition, the cellulose having a crystallinity of 45 or less or a derivative thereof may be used without limitation as long as it satisfies the crystallinity range, for example, GB-1, GB-1W having a crystallinity of 0 or W- having a crystallinity of 40 to 45. Cellulose having a code name of 100 GK.

In a preferred embodiment, the mixture of cellulose having a degree of crystallinity of 60 or a derivative thereof and cellulose or a derivative thereof having a degree of crystallinity of 45 or less is 95: 5 as a weight ratio of cellulose or a derivative thereof having a crystallinity of 60 or greater: To 5: 95, preferably 80: 20 to 20: 80, more preferably 1: 1 may be mixed, but is not limited thereto.

As described above, in the case of the solid priming matrix according to the present invention including a mixture of cellulose having a degree of crystallinity of more than 60 or a cellulose or derivative thereof having a degree of crystallization of 45 or less, the total crystallinity of cellulose in the matrix is not limited. Preferably less than 65.

In addition, the cellulose derivative may be a known cellulose derivative without any limitation, for example, cellulose triacetate, cellulose diacetate or a mixture thereof may be used.

The size of the particles of the cellulose or the cellulose derivative in the solid priming matrix according to the present invention is not particularly limited, but the average particle size in the dry state without absorbing moisture is preferably 1 μm or more and less than 1 mm, preferably 5 μm or more and less than 200 μm, more preferably. Preferably it can be more than 20μm and less than 150μm.

When using a mixture of cellulose having a degree of crystallinity greater than 60 and a cellulose having a degree of crystallization of 45 or less or a derivative thereof in a solid priming matrix, it has a merit that it adheres well to the seed surface without harming the seed by a large surface area without being dissolved in water. Not only environmentally friendly. By using a cellulose having a high water content by the hydrophilic group, it is possible to maintain a constant moisture potential of the seed to provide a uniform and high germination rate of the seed.

As another aspect, the present invention relates to a composition for seed vitality treatment comprising a solid priming matrix comprising a mixture of cellulose having a degree of crystallinity greater than 60 and cellulose having a degree of crystallization of 45 or less or a derivative thereof and water.

The solid priming matrix preferably contains 10 wt% to 300 wt%, preferably 50 wt% to 200 wt% of the seed.

When the solid priming matrix is included outside the above range, it may not be possible to maintain the moisture potential of the seed, which may indicate a problem that the germination rate is lowered. When the amount of water used for priming is equal to 50% by weight relative to the seed, when the solid priming matrix according to the present invention contains less than 10% by weight, the seeds are supplied with a lot of water to induce the degeneration of the seeds and the different Solid priming effect due to the introduction of cellulose having a crystallinity or a derivative thereof may not be sufficiently exhibited, so that the seed vitality improving effect may be insignificant or the seed vitality may not be improved, and when it contains more than 300% by weight, the seed germination rate is insufficient because the seed is not supplied with sufficient moisture. This has the disadvantage of being degraded.

Furthermore, the composition according to the invention comprises water for the solid priming treatment, preferably comprising at least 0.1% by weight and less than 5% by weight, preferably less than 2% by weight, of inorganic salts relative to the weight of the total water. It is characterized by. The type of water is not particularly limited, whether constant or purified water, characterized in that the content of the inorganic salts as described above. The inorganic salts are preferably one or two or more selected from the group consisting of NaCl, KNO ₃ , K ₃ PO ₄ , KH ₂ PO _4, and MgSO ₄ . The composition according to the invention is characterized in that it exhibits such a low content of inorganic salts, but also shows an excellent seed vitality improving effect in combination with the solid priming matrix. In a preferred embodiment, the water having the above inorganic salt content in the composition according to the present invention may be included in an amount of 10 to 300% by weight, preferably 50 to 150% by weight, based on the total mass of the seeds.

In another aspect, the present invention relates to a seed vitality improving treatment method comprising the following steps and a seed treated thereby:

(a) mixing the seed prior to sowing and the composition for treating seed vitality including the solid priming matrix and water, and storing the mixture; And

(b) washing the mixture stored in step (a) to obtain seeds and drying them.

In a specific embodiment, the seed used in the seed vitality improving method according to the present invention can be used without limitation as long as it is a seed that requires vitality improving as a seed before sowing. Watermelon or pumpkin. In one specific embodiment, a solid priming matrix comprising a combination of cellulose having a specific range of crystallinity and water having a specific inorganic salt content were treated with a mixture of sorghum seeds (trade name: Palmhann Concentration No. 3).

Seed vitality enhancing treatment comprising a solid priming matrix comprising a mixture of cellulose having a degree of crystallinity of greater than 60 or a cellulose or derivative thereof having a degree of crystallization of 45 or less and a mixture of two or more thereof and water having an inorganic salt of less than 5% by weight. Regarding the composition for application, the same matters as described above may be applied.

Seed vitality enhancing treatment comprising a solid priming matrix comprising a mixture of cellulose having a degree of crystallinity of greater than 60 or a cellulose or derivative thereof having a degree of crystallization of 45 or less and a mixture of two or more thereof and water having an inorganic salt of less than 5% by weight. After the treatment of the composition for treatment, the composition is mixed with seeds and treated, preferably sealed and stored for 3 to 7 days to be treated. In addition, such treatment is preferably performed at a temperature of 15 to 30 ° C. under dark conditions, and more preferably at 20 ° C. under dark conditions, but is not limited thereto. If the temperature is less than 15 ° C., the priming effect may be weak, and if the temperature exceeds 30 ° C., the seeds may deteriorate.

Thus, the seed treated and stored in the composition for improving seed vitality may be obtained by washing with water or the like and then dried by an appropriate method. The drying method may be a known drying method such as natural drying without limitation, the drying temperature and time may vary depending on the drying method, in the case of natural drying may be 20-30 hours at room temperature, preferably 24 hours. .

In addition, the ratio of seed, solid priming matrix, water and inorganic salts may vary depending on various conditions in the seed vitality treatment. Preferably, the weight ratio of seed: solid priming: water: inorganic salts is 100: 20: 50: 0.5 to 100: 40: 100: 1, and more preferably 100: 20: 50: 0.5.

In one embodiment, the present invention relates to a solid priming matrix comprising cellulose or a derivative thereof according to the present invention in a scabbard seed which is a plant of the gourd for treating seed vitality through solid priming according to the present invention and the total water weight. After treating the composition comprising water with 2 to 4% by weight of inorganic salts, the mixture was put in a zipper bag and mixed well, and the air was removed from the zipper bag and stored at 20 ° C. in the dark for 3 to 7 days. After storage, the seeds were washed with water and dried at room temperature for 24 hours. When the soil germination test after drying, the composition and the manufacturing method according to the present invention showed excellent seed germination rate.

Example

Experimental Example 1 Measurement of Crystallinity of Cellulose

In order to measure the crystallinity of the cellulose used in the experiment was carried out as follows.

The crystallinity was measured using Bruker AXS D4 Endeavor XRD, and the measurement conditions were measured at 40kV, 40mA, Cu Ka radiation (wavelength: 1.5418 Å), and LynxEye position sensitive detector. The FDS 0.3 was used to measure the region of 2 theta 10 degrees to 50 degrees for 175 seconds every 0.03 degrees. The crystallinity was calculated using Topas (version. 3), a fitting program. Fitting interval 2theta 10.5 ~ 40 degree, Background was used for 2 ^nd order polynomial peak type was used for Pseudo Voigt (PV) Function. 5 peaks around 2theta ~ 15, ~ 16.5, ~ 20.5, ~ 22.5, ~ 34.5 degrees are considered as crystalline peaks, and the total peak fitting considering 2theta ~ 18.2, ~ 21.7 degrees is considered as an amorphous region Was performed. The actual values were obtained by using the 'Calculate Degree of crystallinity' tab on the TOPAS3 program that estimates the degree of crystallinity from the relative magnitudes of the sum of the regions I_crystal and the sum of the amorphous regions I_amorphous. XRD patterns of cellulose are shown in Figures 2-4.

[Equation 1]

Crystallinity (%) = 100 * I_crystal / (I_crystal + I_amorphous)

The crystallinity values were classified as shown in Table 1 below, and the values of the crystallinity measured according to the above test are shown in Table 2 below.

Crystallinity Less than 30 30 to 35 35 to 40 40 to 45 45-50 50-55 55 to 60 60 to 65 Over 65 sign ① ② ③ ④ ⑤ ⑥ ⑦ ⑧ ⑨

product name CAT No. Crystallinity One Huzhou City Linghu Xinwang (China) PH-101 ⑨ 2 Huzhou City Linghu Xinwang (China) PH-105 ⑧ 3 Golden Bridge Trading Company (Korea) GB-1 ① 4 Nippon Paper (Japan) W-400Y ⑥ 5 Nippon Paper (Japan) W-100GK ④

Experimental Example 2: Measurement of germination rate when using the solid priming matrix and the solid priming method according to the present invention

Seed vitality comprising a solid priming matrix having a mixture of two or more kinds of cellulose having a degree of crystallinity of greater than 60 and a cellulose or derivative thereof having a degree of crystallization of 45 or less and water having an inorganic salt of less than 5% by weight. In order to test the seed germination rate in the case of using the composition for the enhanced treatment and the preparation method using the same, an experiment was conducted according to the following method. Examples 1 to 5 were prepared by mixing cellulose having a crystallinity of 60 or more and a derivative thereof and cellulose or a derivative thereof having a crystallinity of 45 or less in various content ratios, and Comparative Examples 1 to 7 were configured as described in Table 3. .

Specifically, for solid priming, as a solid priming matrix and water and inorganic salts containing bran seed varieties of brand name Daeho No. 3 and cellulose having various degrees of crystallinity KNO ₃ was put together in a zipper bag as shown in the table below and mixed well, and then air was removed from the zipper bag and stored at 20 ° C. at room temperature for 3 to 7 days. After storage, the seeds were washed with water and dried at room temperature for 24 hours. After drying, the priming germination rate of the solids was measured through a top germination test. Germination characteristics were divided into normal, small seedlings, abnormal and ungerminated germination characteristics. Crystallinity of the cellulose used is shown in Table 2 above. The results are shown in Table 3 below.

As a result, as shown in Table 3, in the case of using a solid priming matrix including a mixture of cellulose having a crystallinity greater than 60 or a derivative thereof and cellulose having a crystallinity of 45 or less or a derivative thereof, when using a conventional solid priming matrix such as vermiculite Compared with inorganic salts of very low content, the ratio of abnormal and ungerminated seeds is significantly lower, resulting in an excellent germination rate, and when using a treatment agent such as vermiculite, the ion problem of the treatment agent may affect germination. It can be seen that it does not occur.

Claims (17)

A solid priming matrix comprising a mixture of cellulose having a crystallinity greater than 60 or a derivative thereof and a cellulose having a crystallinity of 45 or less or a derivative thereof. The solid priming matrix of claim 1, wherein the derivative of cellulose is cellulose triacetate, cellulose diacetate or a mixture thereof. The solid priming matrix of claim 1, wherein the average particle size of the cellulose or derivatives thereof is less than 1 mm. The method of claim 1, wherein the weight ratio of the cellulose having a degree of crystallinity of 60 or less: cellulose or a derivative thereof: a cellulose having a degree of crystallinity of 45 or less in a mixture of cellulose or a derivative thereof and a cellulose or a derivative thereof having a crystallinity of 45 or less 95: 5 to 5: 95, the solid priming matrix. The solid priming matrix of claim 1, wherein the total cellulose crystallinity of the mixture of cellulose having a crystallinity of greater than 60 or a derivative thereof and cellulose or a derivative thereof having a degree of crystallinity of 45 or less is less than 65. 5. A composition for treating seed vitality, comprising a solid priming matrix according to any one of claims 1 to 5 and water. The composition for treating seed vitality according to claim 6, wherein the water contains at least 0.1% by weight and less than 5% by weight of inorganic salts based on the total weight of water. The composition of claim 6, wherein the solid priming matrix comprises 10 wt% to 300 wt% of the total weight of the seed. According to claim 6, wherein the water is 10 to 300% by weight relative to the total weight of the seed, the composition. The composition of claim 6, wherein the inorganic salt is one or two or more selected from the group consisting of NaCl, KNO ₃ , K ₃ PO ₄ , KH ₂ PO ₄ and MgSO ₄ . (a) mixing the seed before sowing and the composition for treating seed vitality according to claim 6 and storing the mixture; And
(b) washing the mixture stored in step (a) to obtain seeds and drying them;
Seed vitality treatment method. The method of claim 11, wherein the seed is a seed of crunch, watermelon or pumpkin. The method of claim 11, wherein the storage of step (a) is performed for 3 to 7 days. The method of claim 11, wherein the storage of step (a) is carried out at a temperature of 15 ~ 30 ℃ in the dark conditions. The method of claim 11, wherein the drying in step (b) is carried out by natural drying. The method of claim 11, wherein the natural drying is carried out at room temperature for 20 to 30 hours. Seed treated by the method according to claim 11.

Images