KR20190063245A - Breast cancer treatment and breast reconstruction device using nano-fiber - Google Patents

Breast cancer treatment and breast reconstruction device using nano-fiber Download PDF

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KR20190063245A
KR20190063245A KR1020170162190A KR20170162190A KR20190063245A KR 20190063245 A KR20190063245 A KR 20190063245A KR 1020170162190 A KR1020170162190 A KR 1020170162190A KR 20170162190 A KR20170162190 A KR 20170162190A KR 20190063245 A KR20190063245 A KR 20190063245A
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김철생
박찬희
아라띠람
아피쉬
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전북대학교산학협력단
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Abstract

The present invention relates to a breast cancer treatment and breast reconstruction device using nanofibers. Specifically, the present invention relates to a breast cancer treatment and breast reconstruction device using nanofibers, capable of reconstruct incised breasts by undergoing chemotherapy and regenerating fat after breast cancer surgery by incision. In the breast cancer treatment and breast reconstruction device using nanofibers of the present invention, as a breast cancer treatment device that is inserted around a breast cancer lesion for the treatment of breast cancer, a nanofiber body inserted around the breast cancer lesion has chemotherapeutic agents and pre-adipocytes attached thereto while the pre-adipocytes regenerate adipocytes and fill the site where the breast cancer is removed therewith.

Description

나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스 { BREAST CANCER TREATMENT AND BREAST RECONSTRUCTION DEVICE USING NANO-FIBER }TECHNICAL FIELD [0001] The present invention relates to a breast cancer treatment device and a breast restoration device using nanofibers,

본 발명은 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스에 관한 것으로서, 특히 절개에 의한 유방암 수술 후 항암치료를 하고 지방을 재생시켜 절개된 유방을 복원시킬 수 있는 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스에 관한 것이다.The present invention relates to a breast cancer treatment and breast restoration device using nanofibers, and more particularly, to a breast cancer treatment and breast restoration device using nanofibers capable of restoring incised breast by regenerating fat by chemotherapy after incisional breast cancer surgery .

전 세계적으로 암을 정복하기 위해 많은 과학자 및 연구자들이 많은 노력을 기울이고 있다.Many scientists and researchers are working hard to conquer cancer worldwide.

암은 일반 세포와 다르게 비 이상적으로 빠른 속도로 성장하고 정상적인 세포에 영향을 주고 암을 치료한다 해도 재발 되거나 암이 전이된다는 점이다.Cancer, unlike normal cells, grows at a rate that is abnormally high, affects normal cells, and even if cancer is treated, recurrence or cancer is transferred.

또한 암 치료 중에 암뿐만 아니라 일반 세포에도 타격을 입기 때문에 섣불리 치료를 진행하기 힘들다. In addition, since cancer cells as well as cancer cells are damaged during cancer treatment, it is difficult to proceed with the treatment.

이러한 이유 때문에 매년 암으로 인한 사망자 수가 늘어나고 있다. For these reasons, the number of deaths from cancer is increasing every year.

종류 또한 다양한 형태로 사망자를 나게 만들고, 암으로 인한 사망자 수가 매년 증가하고 있는 현실이다.Kinds also make death in various forms, and cancer deaths are increasing every year.

그렇기 때문에 보다 효과적인 암치료 및 진단을 가능케 하는 약물 전달체 내지 진단 소재의 개발이 절실히 필요한 실정이다.Therefore, the development of a drug delivery system or a diagnostic material capable of more effective cancer treatment and diagnosis is urgently needed.

암을 치료하는 방법으로는, 약물치료와 열치료가 대표적인데, 종래에는 약물치료와 열치료를 함께 하지 못하고 각각 별개로 치료를 하여야만 하는 한계가 있었다.As a method of treating cancer, drug therapy and thermotherapy are representative. In the past, there has been a limit in which medicines and therapies can not be combined with each other.

이러한 문제점을 해결하기 위해, 본 발명의 출원인은 공개특허 제10-2017-0070978호를 통해 '약물방출 및 열치료 기능을 갖는 스텐트와 이를 이용한 체외 전자기장 구동형 치료 시스템'를 개발하였다.In order to solve these problems, the applicant of the present invention developed a stent having a drug releasing and heat-treating function and an extracorporeal electromagnetic field-driven therapeutic system using the stent, through a patent document 10-2017-0070978.

한편, 유방암의 경우에는 수술에 의한 암세포의 제거시 유방의 일부도 절개하여 제거하게 되는바, 수술 이후에 인공물질 등을 이용하여 유방의 형상을 인공적으로 형성하고 있는 실정이다. On the other hand, in the case of breast cancer, when the cancer cells are removed by surgery, a part of the breast is also removed, and after the surgery, the shape of the breast is artificially formed using artificial materials and the like.

공개특허 제10-2017-0070978호Published Japanese Patent Application No. 10-2017-0070978

본 발명은 유방암의 수술 후 항암치료제로 암세포를 치료를 하고 지방선구세포를 이용하여 지방세포를 재생시킴으로서 수술에 의해 절개된 유방을 인공물질이 아닌 자연적으로 원래의 형상대로 복원시킬 수 있는 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스를 제공하는데 그 목적이 있다.The present invention relates to a nano-fiber capable of naturally restoring a mammalian breast that has been excised by an operation to an original shape by regenerating adipocytes by treating cancer cells with an anti-cancer therapeutic agent after breast cancer surgery, And to provide a breast cancer treatment and breast restoration device using the same.

상기 목적을 달성하기 위하여 본 발명의 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스는, 유방암의 치료를 위해 유방암 병변 주위에 삽입되는 유방암 치료 디바이스에 있어서, 유방암 병변 주위에 삽입되는 나노섬유체에는 항암치료제와 지방선구세포(Pre-adipocyte)가 부착되어 있되, 상기 지방선구세포는 유방암이 제거된 자리에서 지방세포를 재생시켜 충진시키는 것을 특징으로 한다.In order to achieve the above object, the present invention provides a breast cancer treatment and breast restoration device using nanofibers, which is inserted into a breast cancer surrounding the breast lesion for treatment of breast cancer, wherein the nanofiber body inserted around the breast cancer lesion is provided with an anti- And adipocytes are adhered to the adipocytes. The adipocytes are regenerated and filled with adipocytes at the site where the breast cancer is removed.

상기 항암치료제는 항암약물로 이루어진다.The anticancer therapeutic agent is composed of an anticancer drug.

상기 항암약물은 그래핀 옥사이드 독소루비신(Graphene oxide doxorubicin)이다.The anticancer drug is graphene oxide doxorubicin.

또는, 상기 항암치료제는 외부의 자기장발생기에서 발생되는 자기장에 의해 발열되는 발열나노입자로 이루어진다.Alternatively, the anticancer therapeutic agent is composed of exothermic nanoparticles generated by a magnetic field generated by an external magnetic field generator.

또는 상기 항암치료제는, 그래핀 옥사이드 독소루비신(Graphene oxide doxorubicin)로 이루어진 항암약물과, 외부의 자기장발생기에서 발생되는 자기장에 의해 발열되는 발열나노입자로 이루어진다.Alternatively, the anticancer therapeutic agent is composed of an anticancer drug composed of graphene oxide doxorubicin and a heat generating nanoparticle generated by a magnetic field generated from an external magnetic field generator.

상기 지방선구세포는, 상기 항암약물의 방출 이후에 상기 나노섬유체에 주입되어 결합된다.The adipose precursor cells are injected and bound to the nanofiber body after the release of the anti-cancer drug.

이상에서 설명한 바와 같은 본 발명의 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스에 따르면 다음과 같은 효과가 있다.According to the breast cancer treatment and breast restoration device using the nanofibers of the present invention as described above, the following effects can be obtained.

본원발명은 나노섬유체를 유방암의 병변 주위에 삽입하여 나노섬유체에 결합되어 있는 항암치료제로 항암치료를 하고, 지방선구세포를 이용하여 절개된 유방에 지방세포를 유도/증식시킴으로서 유방의 형상을 인공물질이 아닌 자연적으로 원래의 형상대로 복원시킬 수 있다. The present invention relates to a method for treating breast cancer by introducing a nanofiber body around a lesion of a breast cancer and chemotherapy with an anti-cancer therapeutic agent bound to a nanofiber body and inducing / proliferating an adipocyte in an incisional breast using a fat precursor cell, It is not an artificial material but can be restored to its original shape naturally.

본 발명에서는 지방선구세포를 나노섬유체에 주입시키기 때문에, 지방선구세포에 의한 지방세포의 증식이 보다 신속하고 잘 이루어질 수 있다.In the present invention, since adipose precursor cells are injected into the nanofiber body, proliferation of adipocytes by lipid precursors can be performed more quickly and well.

특히, 항암약물의 방출 이후에 지방선구세포를 나노섬유체에 주입시킴으로써, 지방세포의 재생이 보다 신속하게 이루어져 절개된 유방이 원상태로 신속하게 복원되도록 할 수 있다.In particular, by injecting adipose precursor cells into the nanofiber body after the release of the anti-cancer drug, the regeneration of the adipocyte can be performed more quickly and the incisional breast can be restored to its original state quickly.

도 1은 본 발명의 실시예에 따른 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스를 이용하여 암세포를 제거하는 과정을 도시한 순서도,
도 2는 도 1에 의한 암세포 제거 후 지방선구세포를 주입하여 유방을 복원하는 과정을 도시한 순서도,
도 3은 본 발명의 실시예에 따른 디바이스에 의해 지방세포의 증식여부를 설명하기 위한 그래프,
도 4는 도 3의 각 상태에서 지방세포를 촬영한 사진,
도 5는 도 3에서 본 발명의 디바이스에 의한 지방세포 증식상태를 촬영한 FESEM 사진.1 is a flowchart illustrating a process of removing cancer cells using a nanofiber-based breast cancer treatment device and a breast recovery device according to an embodiment of the present invention;
FIG. 2 is a flowchart showing a process of breast reconstruction by injecting adipose precursor cells after cancer cell removal according to FIG. 1; FIG.
3 is a graph illustrating the proliferation of adipocytes by a device according to an embodiment of the present invention,
FIG. 4 is a photograph of fat cells taken in each state of FIG. 3,
Fig. 5 is a FESEM photograph of the fat cell proliferation state by the device of the present invention in Fig. 3; Fig.

본 발명의 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스는 유방암의 치료를 위해 유방암 병변 주위에 삽입되는 것으로써, 나노섬유체(10)와 항암치료제(20)와 지방선구세포(30, Pre-adipocyte)로 이루어진다.The breast cancer treatment and breast restoration device using the nanofibers of the present invention is inserted around the breast cancer lesion for the treatment of breast cancer, and the nanofiber body 10, the chemotherapeutic agent 20, the pre-adipocyte 30, ).

상기 나노섬유체(10)는 종래의 공지된 전기방사장치 등을 이용하여 제조되는 나노섬유매트 등으로 이루어진다.The nanofiber body 10 is made of a nanofiber mat or the like, which is manufactured using a conventional known electrospinning apparatus.

이러한 상기 나노섬유체(10)는 유방암 병변 주위에 삽입된다.The nanofiber body 10 is inserted around a breast cancer lesion.

상기 항암치료제(20)는 상기 나노섬유체(10)에 부착되어 있다.The anti-cancer therapeutic agent (20) is attached to the nanofiber body (10).

이러한 상기 항암치료제(20)는 종래의 공지된 다양한 것으로 이루어질 수 있다.Such an anticancer therapeutic agent 20 may be a variety of known conventional agents.

본 실시예에서 상기 항암치료제(20)는 항암약물(21) 및/또는 발열나노입자(22)로 이루어진다.In the present embodiment, the anticancer therapeutic agent 20 is composed of the anticancer drug 21 and / or the exothermic nanoparticles 22.

상기 항암약물(21)은 암세포(40)를 약물로 치료하는 것이고, 상기 발열나노입자(22)는 암세포(40)를 열로 치료하는 것이다.The anticancer drug 21 is to treat the cancer cells 40 with a drug, and the exothermic nanoparticles 22 treat the cancer cells 40 with heat.

상기 항암치료제(20)는 상기 나노섬유체(10)를 제조하기 위한 고분자에 혼합되어 전기 방사함으로써 상기 나노섬유체(10)에 부착되어 있을 수도 있고, 상기 나노섬유체(10)를 제조한 다음 후공정을 통해 상기 나노섬유체(10)에 부착시킬 수도 있다.The anticancer therapeutic agent 20 may be adhered to the nanofiber body 10 by mixing with a polymer for producing the nanofiber body 10 and electrospunning the nanofiber body 10, And may be attached to the nanofiber body 10 through a post-process.

상기 항암치료제(20)를 구성하는 상기 항암약물(21)은 종래의 공지된 다양한 약물로 이루어질 수 있고, 본 발명과 같이 그래핀 옥사이드 독소루비신(Graphene oxide doxorubicin)로 이루어질 수도 있다.The anticancer drug 21 constituting the anticancer therapeutic agent 20 may be composed of various drugs known in the art and may be composed of graphene oxide doxorubicin as in the present invention.

상기 나노섬유체(10)에 부착되는 상기 항암약물(21)이 그래핀 옥사이드 독소루비신(Graphene oxide doxorubicin)로 이루어짐으로써, 항암효과를 더욱 향상시킬 수 있다.The anticancer drug 21 attached to the nanofiber body 10 is made of graphene oxide doxorubicin, so that the anticancer effect can be further improved.

상기 항암치료제(20)를 구성하는 상기 발열나노입자(22)는 외부의 자기장발생기(50)에서 발생되는 자기장에 의해 발열되는 특성을 갖고 있는 것으로써, 산화철나노입자로 이루어짐이 바람직하다.The exothermic nanoparticles 22 constituting the anticancer therapeutic agent 20 have a characteristic of generating heat by a magnetic field generated by an external magnetic field generator 50 and are preferably made of iron oxide nanoparticles.

그리고, 상기 산화철나노입자의 표면에는 코팅층이 코팅되어 있다.The surface of the iron oxide nanoparticles is coated with a coating layer.

상기 코팅층은, 2-하이드록시에틸 메타크릴레이트(2-Hydroxyethyl methacrylate) 단량체 및 도파민 메타크릴아미드(Dopamine methacrylamide) 단량체를 중합시킨 공중합체이다.The coating layer is a copolymer obtained by polymerizing a 2-hydroxyethyl methacrylate monomer and a dopamine methacrylamide monomer.

여기에서, 2-하이드록시에틸 메타크릴레이트(2-Hydroxyethyl methacrylate, HEMA)는 빛과 수분에 민감하며 단량체는 친수성의 성질을 가진다.Here, 2-hydroxyethyl methacrylate (HEMA) is sensitive to light and moisture, and the monomer is hydrophilic.

또한, 도파민메타크릴아미드(Dopaminemethacrylamide, DMA)는 3,4-dihydroxyphenylalanine(3,4-디히드옥시페닐알라닌, Dopa)의 특성을 가지고 있다.Dopaminemethacrylamide (DMA) also has the properties of 3,4-dihydroxyphenylalanine (3,4-dihydroxyphenylalanine, Dopa).

상기 3,4-디히드옥시페닐알라닌이 금속산화물과 강한 상호작용을 할 수 있고, 간단한 담금질(immersion)을 통해 폴리도파민(polydopamine)을 가진 다양한 물질들의 표면개질로 표면특성들을 크게 변화시킬 수 있고, 금속산화물(metal oxides)과 상호작용을 가능하게 할 수 있다.The 3,4-dihydroxyphenylalanine can strongly interact with the metal oxide and the surface characteristics of the various materials having polydopamine can be greatly changed by simple immersion, And may enable interaction with metal oxides.

상기 항암치료제(20)는 항암약물(21) 또는 발열나노입자(22) 중 어느 하나만으로 이루어져 상기 나노섬유체(10)에 결합될 수도 있으나, 항암약물(21)과 발열나노입자(22)가 함께 상기 나노섬유체(10)에 결합되어 항암약물(21)에 의한 약물치료와 발열나노입자(22)에 의한 열치료를 병행하여 수행할 수 있도록 함이 바람직하다.The anticancer therapeutic agent 20 may be composed of only one of the anticancer drug 21 and the exothermic nanoparticles 22 and may be bound to the nanofiber body 10, It is preferable that the nanoparticles are combined with the nanofiber body 10 so that the treatment with the anti-cancer drug 21 and the heat treatment with the exothermic nanoparticles 22 can be performed in parallel.

상기 지방선구세포(30, Pre-adipocyte)는 상기 나노섬유체(10)에 부착되어 있다.The pre-adipocyte 30 is attached to the nanofiber body 10.

이러한 상기 지방선구세포(30)는 유방 암세포(40)가 제거된 자리에서 지방세포(35)를 유도/재생시켜 절개된 유방의 일부를 복원시키는 역할을 한다.The adipose precursor cells 30 induce / regenerate the adipocytes 35 at the site where the breast cancer cells 40 are removed, thereby restoring a part of the incised breast.

상기 지방선구세포(30)는 상기 항암치료제(20)와 함께 상기 나노섬유체(10) 부착되어 있을 수도 있고, 상기 항암치료제(20)에 의한 항암치료 이후에 상기 나노섬유체(10)에 별도로 주입되어 결합될 수도 있다.The adipose precursor cells 30 may be attached to the nanofiber body 10 together with the anticancer therapeutic agent 20 or may be separately attached to the nanofiber body 10 after the chemotherapy treatment with the anti- And may be injected and bonded.

바람직하게는, 상기 나노섬유체(10)에 부착되어 있는 상기 항암치료제(20)에 의한 항암치료 이후에, 상기 지방선구세포(30)가 상기 나노섬유체(10)에 별도로 결합되도록 한다.Preferably, the adipose precursor cells 30 are separately bound to the nanofiber body 10 after chemotherapy by the chemotherapeutic agent 20 attached to the nanofiber body 10.

위와 같이 항암치료 후 상기 지방선구세포(30)를 상기 나노섬유체(10)에 결합시키게 되면, 후술하는 바와 같이 암세포(40)가 제거된 부분에서 지방세포(35)의 증식이 보다 신속하게 이루어져, 암세포(40) 절개 이후에 유방의 형상을 거의 원상태로 복원시킬 수 있다.When the adipose precursor cells 30 are bound to the nanofiber body 10 after the chemotherapy as described above, the proliferation of the adipocytes 35 is more rapidly performed at the site where the cancer cells 40 are removed as described later , The shape of the breast can be restored to its original state after the incision of the cancer cells (40).

이하에서는, 본원발명인 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스를 이용하여 치료하는 과정과 그에 따른 실험결과데이터 등을 기초로 설명한다.Hereinafter, the present invention will be described on the basis of a process of treating breast cancer using nanofibers and a breast restoration device, and data of experimental results thereof.

도 1(a)에 도시된 바와 같이 유방에 암세포(40)가 있는 경우, 먼저 암세포(40)를 절개하여 제거한다.When the cancer cells 40 are present in the breast as shown in FIG. 1 (a), the cancer cells 40 are first cut and removed.

그 후 도 1(b)에 도시된 바와 같이 암세포(40)가 제거된 자리 즉 유방암 병변 주위에 본 발명의 디바이스를 삽입 배치한다.Then, as shown in FIG. 1 (b), the device of the present invention is inserted and disposed around the site where the cancer cells 40 have been removed, that is, the breast cancer lesion.

이때 상기 디바이스는 나노섬유체(10)에 항암약물(21) 및 발열나노입자(22)로 이루어진 상기 항암치료제(20)가 부착되어 이루어진다.At this time, the device is formed by attaching the anticancer therapeutic agent 20 made of the anticancer drug 21 and the exothermic nanoparticles 22 to the nanofiber body 10.

상기 항암약물(21)을 특정 약물을 방출시켜 항암치료를 한다.The anticancer drug (21) is released into a specific drug to perform chemotherapy.

그리고, 외부에 배치된 자기장발생기(50)에서 자기장을 인가하게 되면 상기 발열나노입자(22)는 발열되어 열치료를 하게 된다.When a magnetic field is applied to the externally disposed magnetic field generator 50, the exothermic nanoparticles 22 generate heat and perform heat treatment.

즉, 도 1(c)에 도시된 바와 같이, 상기 디바이스에 의해 암세포(40)를 열치료와 약물치료를 병행하게 된다.That is, as shown in Fig. 1 (c), the cancer cell 40 is subjected to heat treatment and drug treatment by the device.

상기 항암치료제(20)에 의해 항암제가 모두 방출되고 암세포(40)가 제거 완료되면, 상기 나노섬유체(10)에 상기 지방선구세포(30)를 추가한다.When all the anticancer drugs are released by the anticancer therapeutic agent 20 and the cancer cells 40 are completely removed, the adipose precursor cells 30 are added to the nanofiber body 10.

상기 지방선구세포(30)를 상기 나노섬유체(10)에 추가하는 방법으로는 도 2(a)에 도시된 바와 같이 주사기 등을 이용하여 약물이 모두 방출된 상기 나노섬유체(10)에 상기 지방선구세포(30)를 주입하여 결합시킨다.As the method of adding the lipid precursor cells 30 to the nanofiber body 10, as shown in FIG. 2 (a), the nanofiber body 10, from which the drug has been completely released, The fat precursor cells 30 are injected and bound.

상기 나노섬유체(10)에 부착된 상기 지방선구세포(30)는 도 2(b)에 도시된 바와 같이, 지방세포(35)를 유도/재생시켜 절개된 유방의 일부를 거의 원상태로 복원시키게 된다.As shown in FIG. 2 (b), the adipose precursor cells 30 attached to the nanofiber body 10 induce / regenerate the adipocyte 35 to restore a part of the incised breast to its original state do.

도 3는 본 발명의 실시예에 따른 디바이스에 의해 지방세포(35)의 증식여부를 설명하기 위한 그래프이고, 도 4는 도 3의 각 상태에서 지방세포(35)를 촬영한 사진이다.FIG. 3 is a graph for explaining the proliferation of the adipocyte 35 by the device according to the embodiment of the present invention, and FIG. 4 is a photograph of the adipocyte 35 taken in each state of FIG.

도 3 및 도 4에서는 지방세포(35)에 형광물질을 주입하여 지방세포(35)의 증식여부를 실험한 것이다.In FIGS. 3 and 4, experiments were conducted to determine whether or not the adipocyte 35 was proliferated by injecting a fluorescent substance into the adipocyte 35.

도 3에서 샘플1은 본 발명의 실시예에 따른 나노섬유체(10)에 지방선구세포(30)가 주입되지 않은 것이고, 샘플2는 나노섬유체(10)에 항암치료제(20)와 지방선구세포(30)가 함께 주입되는 것이며, 샘플3은 항암약물(21)이 모두 방출된 이후에 나노섬유체(10)에 지방선구세포(30)를 주입한 것이다.3, Sample 1 shows that the adipose precursor cells 30 are not injected into the nanofiber body 10 according to the embodiment of the present invention and Sample 2 shows that the nanofiber body 10 has the chemotherapeutic agent 20, The cells 30 are injected together and the sample 3 is injected with the adipose precursor cells 30 into the nanofiber body 10 after all the anticancer drugs 21 have been released.

도 4는 실험 14일째에 지방세포(35)의 증식상태를 촬영한 사진으로써, 도 4(a)는 샘플1의 지방세포(35) 증식상태를 촬영한 것이고, 도 4(b)는 샘플2의 지방세포(35) 증식상태를 촬영한 것이며, 도 4(c)는 샘플3의 지방세포(35) 증식상태를 촬영한 것이다.4 is a photograph of the proliferation state of the adipocyte 35 at the 14th day of the experiment. Fig. 4 (a) is a photograph of the proliferation of the adipocyte 35 of the sample 1, and Fig. 4 FIG. 4 (c) is a photograph of the proliferation state of the adipocytes 35 of the sample 3. FIG.

도 3 및 도 4로부터 본 발명의 나노섬유체(10)에 지방선구세포(30)를 주입하면, 지방선구세포(30)가 없는 경우보다 지방세포(35)의 증식이 원활하게 이루어지는 것을 확인할 수 있고, 특히 항암약물(21)이 모두 방출된 이후에 나노섬유체(10)에 지방선구세포(30)를 주입한 샘플3의 경우에 지방세포(35)의 재생/증식이 가장 원활하게 이루어지는 것을 확인할 수 있다.3 and 4, when the adipose precursor cells 30 were injected into the nanofiber body 10 of the present invention, it was confirmed that the proliferation of the adipocytes 35 was more smooth than that without the adipose precursor cells 30 In particular, in the case of the sample 3 in which the adipose precursor cells 30 are injected into the nanofiber body 10 after all the anti-cancer drug 21 is released, the regeneration / proliferation of the adipocyte 35 is most smoothly performed Can be confirmed.

도 5은 도 3에서 본 발명의 디바이스에 의한 지방세포(35) 증식상태를 촬영한 FESEM 사진으로써, 도 5(a) 및 (b)는 각각 샘플2의 4일째 사진과 8일째 사진이며, 도 5(c) 및 (d)는 각각 샘플3의 4일째 사진과 8일째 사진이다FIG. 5 is a FESEM photograph showing the state of proliferation of the adipocytes 35 by the device of the present invention in FIG. 3. FIG. 5 (a) and FIG. 5 (b) 5 (c) and (d) are photographs of the fourth day and fourth day of sample 3, respectively

도 5을 살펴보면, 시간이 지남에 따라 지방세포(35)의 증식이 잘 이루어지고 있다는 것을 확인할 수 있다.Referring to FIG. 5, it can be confirmed that the proliferation of the adipocytes 35 is progressing with time.

위와 같이, 본원발명은 나노섬유체(10)를 유방암의 병변 주위에 삽입하여 나노섬유체(10)에 결합되어 있는 항암치료제(20)로 항암치료를 하고, 지방선구세포(30)를 이용하여 절개된 유방에 지방세포(35)를 유도/증식시킴으로서 유방의 형상을 원래의 형상으로 복원시킬 수 있다.As described above, the present invention is characterized in that the nanofiber body 10 is inserted around the lesion of the breast cancer to perform chemotherapy with the anticancer therapeutic agent 20 bound to the nanofiber body 10, By inducing / multiplying the fat cells 35 in the incised breast, the shape of the breast can be restored to its original shape.

특히, 본 발명에서는 지방선구세포(30)를 나노섬유체(10)에 주입시키기 때문에, 지방선구세포(30)에 의한 지방세포(35)의 증식이 보다 신속하고 잘 이루어질 수 있다.Particularly, in the present invention, since the fat precursor cells 30 are injected into the nanofiber body 10, the proliferation of the fat cells 35 by the fat precursor cells 30 can be performed more quickly and easily.

본 발명인 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스는 전술한 실시예에 국한하지 않고, 본 발명의 기술 사상이 허용되는 범위 내에서 다양하게 변형하여 실시할 수 있다.The breast cancer treatment and breast restoration device using nanofibers of the present invention is not limited to the above-described embodiments, and various modifications can be made within the scope of the technical idea of the present invention.

10 : 나노섬유체, 20 : 항암치료제, 21 : 항암약물, 22 : 발열나노입자, 30 : 지방선구세포, 35 : 지방세포, 40 : 암세포, 50 : 자기장발생기.10: nanofiber body, 20: chemotherapeutic agent, 21: anticancer drug, 22: pyrogenic nanoparticle, 30: fat precursor cell, 35: fat cell, 40: cancer cell, 50: magnetic field generator.

Claims (6)

유방암의 치료를 위해 유방암 병변 주위에 삽입되는 유방암 치료 디바이스에 있어서,
유방암 병변 주위에 삽입되는 나노섬유체에는 항암치료제와 지방선구세포(Pre-adipocyte)가 부착되어 있되,
상기 지방선구세포는 유방암이 제거된 자리에서 지방세포를 재생시켜 충진시키는 것을 특징으로 하는 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스.A breast cancer treatment device inserted around a breast cancer lesion for the treatment of breast cancer,
The nanofiber body inserted around the breast cancer lesion has chemotherapeutic agent and adipocyte pre-adipocyte,
Wherein the adipose precursor cells regenerate and fill adipocytes at the site where the breast cancer is removed, thereby treating breast cancer and breast restoration using nanofibers. 청구항1에 있어서,
상기 항암치료제는 항암약물로 이루어진 것을 특징으로 하는 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스.The method according to claim 1,
Wherein the anti-cancer therapeutic agent is an anti-cancer drug. 청구항2에 있어서,
상기 항암약물은 그래핀 옥사이드 독소루비신(Graphene oxide doxorubicin)인 것을 특징으로 하는 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스.The method of claim 2,
Wherein the anticancer drug is graphene oxide doxorubicin. ≪ RTI ID = 0.0 > 8. < / RTI > 청구항1에 있어서,
상기 항암치료제는 외부의 자기장발생기에서 발생되는 자기장에 의해 발열되는 발열나노입자로 이루어진 것을 특징으로 하는 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스.The method according to claim 1,
Wherein the anticancer therapeutic agent is composed of exothermic nanoparticles generated by a magnetic field generated by an external magnetic field generator. 청구항1에 있어서,
상기 항암치료제는, 그래핀 옥사이드 독소루비신(Graphene oxide doxorubicin)로 이루어진 항암약물과, 외부의 자기장발생기에서 발생되는 자기장에 의해 발열되는 발열나노입자로 이루어진 것을 특징으로 하는 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스.The method according to claim 1,
Wherein the anticancer therapeutic agent is composed of an anticancer drug composed of graphene oxide doxorubicin and fever nano particles generated by a magnetic field generated from an external magnetic field generator. device. 청구항2에 있어서,
상기 지방선구세포는, 상기 항암약물의 방출 이후에 상기 나노섬유체에 주입되어 결합되는 것을 특징으로 하는 나노섬유를 이용한 유방암 치료 및 유방 복원 디바이스.
The method of claim 2,
Wherein the adipose precursor cells are injected into the nanofiber body after the release of the anticancer drug and bound to the nanofiber body.
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