KR20190061270A - 형질전환 벼 세포 배양에서 자가소화 억제제 처리를 통한 재조합 인간 산 알파-글루코시다제 생산성 증대용 조성물 - Google Patents
형질전환 벼 세포 배양에서 자가소화 억제제 처리를 통한 재조합 인간 산 알파-글루코시다제 생산성 증대용 조성물 Download PDFInfo
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Abstract
Description
도 2는 본 발명의 실시예에 따른 GAAWT 및 GAA gnt1 의 세포 생존도를 나타낸 도이다.
도 3은 본 발명의 실시예에 따른 GAAWT 및 GAA gnt1 의 자가용해소체 형광 염색 결과를 통한 자가소화 진행 정도를 나타낸 도이다.
도 4는 본 발명의 실시예에 따른 GAAWT 및 GAA gnt1 의 세포외 rrhGAA 발현량을 나타낸 도이다.
도 5는 본 발명의 실시예에 따른 GAAWT 및 GAA gnt1 의 총 단백질량 대비 세포내 rrhGAA 발현량을 나타낸 도이다.
작용 기작 | 조절제 종류 | 처리 농도 (μM) |
자가소화 유도제 (Autophagy enhancers) |
라파마이신 (Rapamycin) |
0.1 |
템시롤리무스 (Temsirolimus, CCI-779) |
1 | |
자가소화 억제제 (Autophagy inhibitors) |
콘카나마이신 A (concanamycin A) |
0.05 |
바필로마이신 A1 (bafilomycin A1) |
0.1 |
Claims (12)
- 자가소화 억제제를 포함하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대용 조성물.
- 제 1항에 있어서,
상기 자가소화 억제제는 콘카나마이신 A 또는 바필로마이신 A1인 것을 특징으로 하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대용 조성물.
- 제 2항에 있어서,
상기 콘카나마이신 A은 0.03 내지 0.1 μM 의 농도로 포함되는 것을 특징으로 하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대용 조성물.
- 제 2항에 있어서,
상기 바필로마이신 A1은 0.05 내지 0.3 μM 의 농도로 포함되는 것을 특징으로 하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대용 조성물.
- 자가소화 억제제를 포함하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대용 배지 조성물.
- 자가소화 억제제를 포함하는 배지에서 형질전환 벼 세포를 배양하는 단계;를 포함하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대 방법.
- 제 6항에 있어서,
상기 자가소화 억제제는 콘카나마이신 A 또는 바필로마이신 A1인 것을 특징으로 하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대 방법.
- 제 7항에 있어서,
상기 콘카나마이신 A은 0.03 내지 0.1 μM 의 농도로 포함되는 것을 특징으로 하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대 방법.
- 제 7항에 있어서,
상기 바필로마이신 A1은 0.05 내지 0.3 μM 의 농도로 포함되는 것을 특징으로 하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대 방법.
- 제 6항에 있어서,
상기 배지는 당 고갈 배지인 것을 특징으로 하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대 방법.
- 제 6항에 있어서,
상기 형질전환 벼 세포는 재조합 인간 산 알파-글루코시다제를 생산하는 벼 세포인 것을 특징으로 하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대 방법.
- 제 6항에 있어서,
상기 형질전환 벼 세포는 RAmy3D 유도성 프로모터가 도입된 것을 특징으로 하는, 재조합 인간 산 알파-글루코시다제의 생산성 증대 방법.
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