KR20190057011A - Pest control composition comprising Armeniacae Semen extract as active ingredient - Google Patents

Abstract

The present invention relates to a pest control composition comprising an Armeniacae Semen extract as an active ingredient, to a pest control agent, and to a pest control method using the same. The pest control composition comprising the Armeniacae Semen extract or a fraction thereof as an active ingredient shows a pest control effect minimizing harm to humans and harm to environment by suppressing the activity of receptors of insect-specific small neuropeptides, and thus the pest control agent comprising the pest control composition and the pest control method using the same can be utilized for eco-friendly pest control.

Description

[0001] The present invention relates to a pest control composition comprising Armeniacae Semen extract as active ingredient,

The present invention relates to a pest control composition, an insect pest control composition, and a pest control method using the composition as an active ingredient.

Conventional pest control agents including organic phosphorus have problems such as environmental harm and human harmfulness, and thus use of pesticides including insecticides is limited. As interest in environmentally friendly agricultural products is increased, production of high quality organic agricultural products Farms are increasing. Most pest control technologies that have a significant impact on the production and quality of agricultural products are not eco-friendly or ineffective.

Among the pest control techniques, there is a method of using an insect hormone as a biological control method. Insect hormones are organic compounds that are produced in the body of insects and act effectively in trace amounts. Insect hormones act as chemical messengers that transmit information in and out of the body to specific target cells and regulate the activity of the target cells, allowing insects to maintain their normal life cycle. Insect hormones include neuropeptide hormones synthesized and secreted by neurons in the central nervous system and hormones synthesized and secreted by the endocrine glands.

The common functional groups of neuropeptide hormones in insects are as follows. Neuropeptide hormones bind to receptors in specific target cells with high specificity and affinity to regulate cellular function. In particular, short neuropeptide F, which regulates the various physiological activities of insects, binds to the G protein coupled receptor (GPCR) and activates the adenylate cyclase inside the cell membrane to release cAMP (cyclic AMP) from ATP This cAMP, as a secondary messenger, transmits information to the intracellular enzymes or molecular systems.

Small neuropeptide receptors are known to regulate feeding, reproduction and individual growth by activating various sub-signaling pathways, and are distributed throughout several senses. The present inventors for the first time reported using a Drosophila melanogaster model belonging to the family of Dicloviridae (Drosophila melanogaster ) for the first time that a small neuropeptide, a neuropeptide, modulates the expression of an insulin gene and is involved in metabolic function, individual growth, and lifespan control of Drosophila lee et al., Nature Cell Biol ., 2008), especially PKA-CREB-DYRK path to the latest in sub-signaling pathways in a small neuropeptide reported to induce feeding behavior (Hong et al. PLoS Genetics, 2012).

In particular, insect neuropeptides are very well conserved among insect species, but they differ in amino acid sequence and structure from mammalian neuropeptides with similar functions. Therefore, it is known that compounds or agents that inhibit insect neuropeptide signals do not act on mammalian neuropeptide signals or have very low activity. When insect-specific neuropeptide receptor inhibitors are discovered, But it does not work on mammals, including humans, and can reduce human toxicity.

Armeniacae Semen (杏仁) is an apricot seed which is mainly used as a herb medicine. The apricot tree is a deciduous arboreous tree belonging to the Rosaceae, 5m high. Apricots ripen in yellow or yellowish red in July and taste and sweet. Origin is East Asia. It is not certain when the apricots were cultivated in our country, but it is presumed that they have been naturally grown in mountain areas in the north of the central part of Korea for a long time. There are several varieties of apricot selected from the varieties imported from the US and the domestic lipids in addition to those imported from Japan, such as Peace, Mountain Type 3, Gwangbu Daesil, Digestion and so on. The place of cultivation usually corresponds to the place of apple.

The efficacy of passengers is known as Jinhae, Gadam, Yeomyeom, Pyeongcheon, and Yunjang. Various relief, asthma, bronchitis, acute hepatitis, sore throat, intestinal constipation, · It is used as a remedy for swine fever (carcinoma) and carcinoma (carcinoma). Also, passers-by is known to be good for women's skin beauty.

Regarding passers-by, Korean Patent Registration No. 10-1480700 discloses a cosmetic composition containing a herbal extract comprising a passenger.

As described above, there is no invention of a pest control agent containing an extract of Phellinus linteus, and in particular, there is no known effect of an insect neuron peptide and an extract of Phellinus linteus on the endocrine system.

Under these circumstances, the present inventors have made intensive efforts to develop an environmentally-friendly pest control agent, and as a result, it has been found that the extract of Pseudomonas sp. Effectively modulates the activity of the neuropeptide receptor to reduce insect feeding and increase food avoidance, And confirming that there is almost no toxicity to humans, thereby completing the present invention.

Korean Patent Registration No. 10-1480700

It is an object of the present invention to provide a composition for controlling insects, which comprises an extract or a fraction thereof as an active ingredient.

Another object of the present invention is to provide a pest controlling agent comprising the composition for pest control.

It is still another object of the present invention to provide a pest control method comprising the step of treating the insect pest controlling composition.

It is still another object of the present invention to provide a quasi-drug composition comprising the composition for controlling insects.

Each description and embodiment disclosed in the present application can be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in this application fall within the scope of the present application. Further, the scope of the present application is not limited by the specific description described below.

As one embodiment for achieving the object of the present invention, the present invention provides a composition for pest control comprising an extract or extract thereof as an active ingredient.

In the present invention, it has been confirmed that the extract or its fractions inhibit the activity of small neuropeptide receptors known to regulate feeding, reproduction and individual growth of insects and can be used for pest control.

In the present invention, the term "Armeniacae Semen" refers to apricot trees belonging to the rose family ( Prunus armeniaca Linne var. Ansu Maximowicz ), Prunus mandshurica Koehne var. Glabra Nakai , Prunus sibirica Linne The apricot seed, including the Armenian apricot ( Prunus armeniaca Linne ), is also known as an ancestor, a nucleus or an ascetic.

For the purpose of the present invention, the person is not limited to the kind, and cultivated or commercially available products can be used without limitation in their origin.

In addition, no specific effect on the insect pest control agent is known. Thus, the inventors of the present invention confirmed that the extracts of passerines inhibit the activity of receptors of insect-specific small neuropeptides, reduce insect feeding, and increase the repellency of insects against food containing the insect extract. This is significant in that it is the first time that an environmentally-friendly pest control composition was developed.

The term " extract " in the present invention means an extract obtained by extracting a passenger, a diluted or concentrated liquid of the extracted liquid, a dried material obtained by drying the extracted liquid, a controlled preparation or a purified product of the extracted liquid, And extracts of all formulations that can be formed using an extract.

In the present invention, the passive extract comprises washing, drying and pulverizing passages; And extracting the pulverized starting materials with a solvent selected from the group consisting of water, C ₁ to C ₆ alcohols and mixed solvents thereof. Specifically, the extract can be extracted with methanol, ethanol, propanol, butanol, pentanol or hexanol, and more specifically, extracted with ethanol, but the present invention is not limited thereto.

The extraction method is not particularly limited, and extraction can be carried out at room temperature or with heating under conditions where the active ingredient is not destroyed or minimized. More specifically, the method for obtaining the extract of the present invention is as follows. The dry runner can be used in a polar solvent of a C ₁ to C ₆ alcohol such as water, methanol, ethanol, propanol, butanol, pentanol, hexanol and the like in a volume of about 2 to 20 times, Or a mixed solvent thereof having a mixing ratio of about 1: 0.1 to 1:10 may be used as an elution solvent, but the present invention is not limited thereto. The extraction temperature may be 1 to 100 ° C, specifically 25 ° C, and the extraction period may be about 1 hour to 10 days, specifically, 2 to 50 hours. The extraction method may include shaking extraction, hot water extraction, Reflux cooling extraction, ultrasonic extraction, or a combination thereof. However, the present invention is not limited thereto. In a specific example of the present invention, the pulverized product was immersed in distilled water and extracted at 100 ° C for 150 minutes. The extract was filtered and then lyophilized to obtain an extract from the extract.

The extract can be extracted from natural, hybrid, and variant plants and extracted from plant tissue cultures.

The term " fraction " in the present invention means a product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.

The fractionation method for obtaining the fraction in the present invention is not particularly limited and may be carried out according to a method commonly used in the art. As a non-limiting example of the above-mentioned fractionation method, fractions can be obtained from the extract by treating a predetermined solvent with the extract obtained by extracting passerine.

The kind of the fraction solvent used for obtaining the fraction in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fraction solvent include polar solvents such as water and alcohol; And non-polar solvents such as hexane, ethylacetate, acetone, and chloroform. These may be used alone or in combination of two or more. The ion exchange resin used to fractionate the passenger in the present invention may be a synthetic adsorbent such as aromatic non-substituted resin (diaion HP-20 series, SP825 series, AXT204 series, XAD1600T series, MN200 series).

The term " insect pests " in the present invention refers to all insects which directly or indirectly harm human life. In the present invention, the insect pests may be insects harmful to animals or plants, and may also be human disease-mediated insects, farm insects, horticultural insects and horticultural insects and wooden insects. And inhibiting the activity of the neuropeptide receptor.

Specifically, the pests are selected from the group consisting of Egyptian forest mosquito, white mosquito mosquito, school mosquito, Chinese spotted mosquito, tropical house mosquito, mite, thripworm, Aphids, pea aphids, tobacco may be Louis or potato beetles, but are not limited thereto.

The term " pest control " in the present invention is a series of actions for reducing harm caused by a pest. The pest control method specifically includes physical (mechanical) control, poison control, chemical control, biological control, legal control, But are not limited to, solar heating, hot water impregnation, steam heating, pyrogen heating, infrared cooling, cooling, chopping, ultrasound, embedding, immersion and eco-control.

"Control" is a series of actions to reduce the harm caused by a pest, which can be distinguished from an "insect" that necessarily involves killing the pest. Therefore, using the composition for pest control of the present invention may have little or no effect of disturbing the ecosystem. In addition, since the above-mentioned " control " may include " avoidance ", the composition for controlling insects of the present invention may also be used as a composition for avoiding insects.

In the present invention, for the control of insects, the extract or fraction thereof is present in an amount of 0.5 to 5% by weight, specifically 0.5 to 4% by weight, 0.5 to 3% by weight, 0.5 to 2% by weight, 0.5 to 5% 1 to 5 wt.%, 2 to 5 wt.%, 3 to 5 wt.% Or 4 to 5 wt.%.

(W / w) of the total weight of the composition does not sufficiently inhibit the small neuropeptide receptors of the insect pest, thereby reducing the pest control effect and reducing the pest control effect by more than 5% by weight (w / w) There is a problem in that the production cost of the homogeneous solution preparation and the controlling agent is increased.

The composition for controlling pests may further comprise an extract other than the extract from the passerium. Examples include, but are not limited to, ginseng extract.

The composition for insect control of the present invention can inhibit the activity of an insect-specific neuropeptide receptor, and the neuropeptide may be but is not limited to a short neuropeptide F (sNPF).

The term " short neuropeptide F (sNPF) " in the present invention is a peptide hormone of insects. The receptors of small neuropeptides activate various sub-signaling pathways, regulating insect feeding, reproduction and individual growth It is known and distributed evenly in several senses. The composition for controlling pests may exhibit a controlling effect specifically for insects having activity against small neuropeptides, but the present invention is not limited thereto.

In one embodiment of the present invention, it was confirmed that the extracts of the present invention suppressed the activity of receptors of insect-specific small neuropeptides, resulting in a reduction in the feeding of the fruit flies and an increase in the avoidance of the fruit flies Respectively.

The above results confirm that the extract of Phellinus linteus can be used as a pest control agent.

In another aspect of the present invention, the present invention provides an insect control agent comprising the composition for controlling insects. Pest and pest control are as described above.

In another aspect of the present invention, the present invention provides a pest control method comprising the step of treating the insect pest control composition. Pest and pest control are as described above.

The method may be a method of directly or indirectly treating the insect pest controlling composition of the present invention. However, the insect pest controlling composition may be placed, for example, in the vicinity of humans, animals, Lt; / RTI >

According to another aspect of the present invention, there is provided a quasi-drug composition comprising the above composition for controlling insects.

The quasi-drug is a preparation used for sterilization, insecticide and similar uses for the prevention of the infectious diseases described in the Article 2 (7) of the Pharmaceutical Affairs Act. It is used as an insect repellent for the human or animal health such as flies, mosquitoes, May mean an antiseptic or an attractant insecticide.

The quasi-drug may also include external preparations for skin and personal hygiene products. But is not limited to, a disinfectant cleaner, a shower foam, a guarine, a wet tissue, a detergent soap, a hand wash, or an ointment.

When the composition for controlling insect pests according to the present invention is used as a quasi-drug additive, the composition may be added as it is, or may be used together with other quasi-drugs or quasi-drugs, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be appropriately determined depending on the purpose of use.

The quasi-drug composition of the present invention may be, for example, one prepared in the form of a general emulsified formulation and a solubilized formulation. For example, they may have formulations such as emulsions such as lotions, creams, ointments, sprays, oil gels, gels, oils, aerosols, and softeners, but they can be used without limitation as long as they exhibit the pest control effect of the present invention .

In addition, the quasi-drug composition may be formulated into each formulation as appropriate, if necessary, in an oil, water, surfactant, humectant, lower alcohol having 1 to 4 carbon atoms, a thickener, a chelating agent, a pigment, Can be used.

The composition for insect control comprising the extract of the present invention as an active ingredient or the fraction thereof as an active ingredient exhibits an insect pest control effect with minimized human harmfulness and environmental harmfulness through inhibition of receptor activity of insect-specific small neuropeptides, A pest control agent containing a composition for a pest control agent and a pest control method using the same can be used for environmental pest control.

1 shows a method for measuring the activity of a small neuropeptide and its receptor using a cell line injected with a small neuropeptide receptor expression vector (pAc5.1-sNPFR) and a luciferase expression vector (pGL3-CRE) Fig.
Fig. 2 is a graph showing the inhibitory effect on the small neuropeptide receptor activity of the extract of Panax ginseng.
Fig. 3 is a graph showing the ingestion-inhibiting ability of the goat extract. Fig.
Fig. 4 is a view showing an insect avoidance effect caused by a passerine extract. Fig.
Fig. 5 is a diagram showing the insecticidal efficacy of the goat extract. Fig.
FIG. 6 is a graph showing the non-toxic effect of the extract from the passerine on human neurons. FIG.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. However, these examples and experimental examples are intended to illustrate the present invention, and the scope of the present invention is not limited to these examples and experimental examples.

Example 1: Preparation of Candida extract

The pulverized product was immersed in distilled water and extracted at 100 ° C for 150 minutes. The extract was filtered using an incontinent cotton pad and lyophilized. This construct was dissolved in DMSO at 20 mg / ml and then added to the cell culture medium or insect medium at 1 wt% (w / w) and used in the following examples.

Example 2: Determination of inhibitory activity of small-scale neuropeptide receptor activity of goat extract

Example 2-1: Production of Insect Cell Lines Overexpressing Small Neuropeptide Receptors

In order to analyze the activity of small neuropeptides and their receptors that regulate eating behavior, the efficacy of the goat extract inhibiting small neuropeptide receptor-specific activities using a luminescent assay technique based on the target gene promoter analysis of small neuropeptides .

First, a luciferase expression vector (pGL3-CRE) containing an insect expression vector (pAc5.1-sNPFR) and a CREB response element (CRE) cloned in the fruitfly's small neuropeptide receptor (sNPFR) To produce a cell line capable of measuring the activity of a small neuropeptide receptor.

Treatment of small neuropeptides with the pAc5.1-sNPFR and pGL3-CRE vectors induced PKA-CREB signaling by the over-expressed receptor (sNPFR) and CREB transcription factor binding to pGL3-CRE Luciferase is expressed. When luciferin was treated with luciferin in the cells, luciferin was emitted by luciferase, and the activity of the small neuropeptide and its receptor was measured by measuring the degree of luminescence using a luminometer One).

Example 2-2: Measurement of activity of small neuropeptide receptor

The inhibitory activity against the small neuropeptide receptors of the extracts prepared from the above-mentioned extracts and the extracts of the extracts using the cell lines was confirmed.

Specifically, the degree of luminescence of pAc5.1-sNPFR and pGL3-CRE vector-treated cell lines (control group) treated with small neuropeptide alone and the cell line (experimental group) treated with small neuropeptide and goat extract were compared.

In addition, the effects of passive extracts were evaluated by the combination of the effects of compound insecticides (including insecticides against tobacco and insecticides, including passerine extract, horse extract, peat extract, carrot extract, white pepper extract and malt extract) For comparison, the degree of luminescence of the cell line treated with the compound insecticide or each of its components together with the small neuropeptide was also compared.

As a result, compared with the control group treated with small neuropeptide alone, the experimental group treated with the small neuropeptide and the goat extract decreased the luminescence of luciferin by 38% as compared with the control group, and inhibited the activity of the small neuropeptide receptor in the goat extract (Fig. 2).

In addition, it was confirmed that the inhibitory effect on the small-scale neuropeptide receptor activity of the goat extract was superior to that of the compound insecticide and its constituents (Fig. 2).

Example 3: Measurement of feeding inhibitory ability of goat extract

Example 3-1: Drosophila preparation and breeding

Drosophila was kept at 25 ℃ and used for experiment after rearing in normal medium for 3-5 days after hatching.

Example 3-2: Measurement of feeding amount of fruit fly

The fruit flies were grown for 4 days in a general insect rearing medium or in a rearing medium containing 1% by weight of the extract prepared from Example 1, and after starving for 2 hours, the number of food consumed including food coloring was measured, The intake was quantified by analysis.

In addition, the same experiment as above was carried out using the flies cultured in the growth medium containing the compound insecticide of Example 2-2 or each of the components thereof.

As a result, compared to the control group grown in the general insect rearing medium, the feeding number of the fruit fly grown in the culture medium containing the extract of the passerine was reduced by 77% and the total ingestion amount was decreased by 71% (Fig. 3).

In addition, it was confirmed that the feeding inhibitory effect of the passive extract was superior to that of the compound insecticide and its constituents (Fig. 3).

Example 4: Determination of insect avoidance level for passerine extract

The following experiment was conducted to measure the avoidance degree of the food containing the goat extract.

Specifically, the fruit flies were raised in the same manner as in Example 3, red or blue food coloring matter was added to the rearing medium, and the extract of the passerelle prepared in Example 1 was added only to the blue food coloring medium of the experimental group. The selection tendency was measured by the color change of the fruit fly abdomen, and the degree of avoidance was measured using the following equation.

Avoidance = (Blue-Red) / (Blue + Red + Purple)

As a result, it was confirmed that the avoidance degree of the food supplemented with the goat extract was more than four times higher than that of the control (Fig. 4).

Example 5: Determination of insect lethal effects of goat extract

The following experiment was carried out to confirm the lethal effects of insect extracts on insects.

Specifically, 100 dead fruit flies raised in the same manner as in Example 3 were added to the growth medium supplemented with the extract prepared as in Example 1, and the mortality of the dead extract was determined by confirming the dead number per day.

As a result, it was confirmed that, in the case of Drosophila that was raised in the medium containing the Drosophila extract, 18% of the Drosophila in the whole Drosophila was dead after 5 days of rearing, 5).

Example 6: Determination of toxicity of mammalian extracts

The following experiment was carried out to confirm cytotoxicity of goat extract to mammals.

Specifically, the cell line extract of SK-N-SH, which is a human nerve cell line, was treated for 1 day and the cell survival rate was measured by WST-1 assay, And compared with the control group.

As a result, there was no significant difference in the survival rate of the experimental group treated with the human guinea pig extract and the survival rate of the control group treated with the human guinea pig extract. Therefore, the human guinea pig extract had no toxicity to mammals and was useful as an environmentally friendly pest control agent (Fig. 6).

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all aspects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

Claims (11)

A composition for controlling insects, comprising an extract of Armeniacae Semen or a fraction thereof as an active ingredient.
The composition according to claim 1, wherein the composition inhibits the receptor activity of an insect-specific neuropeptide.
The insect controlling composition according to claim 1, wherein the extract is extracted with a solvent selected from the group consisting of water, an alcohol having 1 to 6 carbon atoms, and a mixed solvent thereof.
4. The composition for controlling insects according to claim 3, wherein the alcohol is ethanol or methanol.
2. The pest controlling composition according to claim 1, wherein the extract or the fraction thereof is contained in an amount of 0.5 to 5 wt% (w / w) based on the total weight of the composition.
The insect controlling composition according to claim 1, wherein the pest is an insect harmful to an animal or a plant.
7. The pest control composition according to claim 6, wherein the pest is any one selected from the group consisting of human disease-mediated insect pests, homestead insect pests, horticultural insect pests and wood insect pests.
The method of claim 7, wherein the pest is selected from the group consisting of Egyptian forest mosquito, white mosquito mosquito, mosquito mite, mite, thrips, brownflies, fruit flies, house flies, fruit flies, grasshoppers, red fire ants and potato beetles By weight of the composition.
9. A pest control agent comprising the composition of any one of claims 1 to 8.
9. A method for controlling insects, comprising the step of treating the composition of any one of claims 1 to 8 to pests.
A quasi-drug composition comprising the pest control composition of any one of claims 1 to 8.

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